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  • Nitrogen fixation
  • Wheat
  • Springer  (40)
  • Periodicals Archive Online (PAO)
  • 1985-1989  (40)
  • 1980-1984
  • 1975-1979
  • 1989  (40)
Collection
Keywords
Publisher
  • Springer  (40)
  • Periodicals Archive Online (PAO)
Years
  • 1985-1989  (40)
  • 1980-1984
  • 1975-1979
Year
  • 1
    Electronic Resource
    Electronic Resource
    Preparation of nitrogen and phosphorus-enriched paddy straw compost and its effect on yield and nutrient uptake by wheat (Triticum aestivum L.) (1989)
    Springer
    Biology and fertility of soils 8 (1989), S. 339-342 
    Details
    ISSN: 1432-0789
    Keywords: Wheat ; Paddy straw compost ; N and P enrichment ; Rock phosphate ; Pyrite ; Triticum aestivum ; Nutrient uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A nutrient-rich compost from paddy straw was prepared using urea and Mussoorie rock phosphate for N and P enrichment respectively. Inorganic N was partly conserved in the compost by the addition of pyrite. Citric-acid-soluble P also increased with the addition of pyrite. Compost containing about 1.6% total N and 3.3% total P was found to be a good source of P for a wheat crop and also supplied a significant amount of N to the plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263166
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  • 2
    Electronic Resource
    Electronic Resource
    Azospirillum — plant root associations: A review (1989)
    Springer
    Biology and fertility of soils 8 (1989), S. 356-368 
    Details
    ISSN: 1432-0789
    Keywords: Plant-root associations ; Azospirillum spp ; Rhizosphere ; Nitrogen fixation ; Acetylene reduction assay (ARA) ; Phytohormones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Bacteria of the genus Azospirillum are extensively studied for their plant-growth promoting effect following inoculation. Physiological and biochemical studies of these diazotrophic bacteria are now benefiting from recent breakthroughs in the development of genetic tools for Azospirilum. Moreover, the identification and cloning of Azospirillum genes involved in N2 fixation, plant interaction, and phytohormone production have given new life to many research projects on Azospirillum. The finding that Azospirillum genes can complement specific mutations in other intensively studied rhizosphere bacteria like Rhizobia will certainly trigger the exploration of new areas in rhizosphere biology. Therefore a review of the Azospirillum-plant interactions is particularly timely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263169
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  • 3
    Electronic Resource
    Electronic Resource
    Impact of nodule damage by Rivellia angulata on N2-fixation, growth, and yield of pigeonpea (Cajanus cajan L. Millsp.) grown in a Vertisol (1989)
    Springer
    Biology and fertility of soils 7 (1989), S. 95-100 
    Details
    ISSN: 1432-0789
    Keywords: Nodule damage ; Rivellia angulata ; Nitrogen fixation ; Cajanus cajan ; Pigeonpea ; Vertisol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Damage caused by Rivellia angulata larvae to pigeonpea root nodules at the ICRISAT center in India was greater in the crop grown on Vertisols (up to 86%) compared to that on Alfisols (20%). Attempts to quantify the field effects of nodule damage on growth and yield of pigeonpea in a Vertisol, involving many heavy applications of soil insecticides (aldrin and hexachlorocyclohexane) failed because the insecticides did not control the pest and adversely affected the growth of the pigeonpea and the subsequent crop of sorghum (Sorgorum bicolor L. Moench). The impact of nodule damage on pigeonpea growth, yield and nutrient uptake was successfully studied in greenhouse-grown plants at three N levels. In this pot study, artificial inoculation with Rivellia sp. led to substantial nodule damage (70%). The results of this damage were a significant overall reduction in nodule dry weight (46%), acetylene reduction activity (31%), total leaf area (36%), chlorophyll content of leaves (39%) and shoot dry weight (23%) 68 days after sowing. At maturity, Rivellia sp. infestation caused significant reductions in top dry weight (22%), root and nodule dry weight (27%), seed dry weight (14%), and total N (29%) and P uptake (19%). The problems and prospects of manipulating nodule damage so as to reduce N losses in pigeonpea are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292565
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of herbicides on three vesicular-arbuscular fungi associated with winter wheat (Riticum aestivum L.) (1989)
    Springer
    Biology and fertility of soils 7 (1989), S. 113-119 
    Details
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhiza ; Herbicides ; Wheat ; Glomus spp. ; Spore germination ; Root infection ; Triticum aestivum L. ; Bifenox ; Mecoprop ; Difenzoquat methyl sulphate ; Chlortoluron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We studied the effects of field application rates of four herbicides (Avenge, Ceridor, Dicurane, and Harrier) on spore germination and infection of wheat roots by three species of Glomus grown under conditions of low P availability. Low concentrations of Ceridor (bifenox, mecoprop) and Harrier (mecoprop, ioxynil, clopyralid) inhibited spore germination while higher concentrations were stimulatory. Avenge (difenzoquat methyl sulphate) prevented spore germination completely, while Dicurane (chlortoluron) had no effect. The herbicide applications had no significant effect on the infection rates of any of the three fungi except Harrier on G. geosporum. The herbicide treatments did, however, affect plant growth and ear yields. Ceridor and Harrier increased ear yields, while Dicurane showed marked phytotoxic effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292568
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  • 5
    Electronic Resource
    Electronic Resource
    Crop growth and nitrogen transformations in wheat (Triticum aestivum L.) planted after wetland rice (Oryza sativa L.) (1989)
    Springer
    Biology and fertility of soils 7 (1989), S. 263-268 
    Details
    ISSN: 1432-0789
    Keywords: Dentrification ; Immobilization ; Tillage ; N leaching ; Wheat ; Wetland rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A field study was undertaken to examine the effects of various management strategies on wheat (Triticum aestivum L.) performance and N cycling in an intensively cropped soil. Microplots receiving 100 kg N ha−1 as15NH4 + 15NO3 − at sowing, tillering or stem elongation were compared with unfertilized microplots. Stubble from the previous rice crop was either incorporated, burnt without tillage, burnt then tilled or retained on the surface of untilled soil. Wheat grain yield ranged from 1.5 to 5.1 t ha− and was closely related to N uptake. Plant accumulation of soil N averaged 36 kg N ha−1 (LSD 5% = 10) on stubble-incorporation plots and 54 kg N ha−1 on stubble-retention plots. Fertilizer N accumulation averaged 18 kg N ha−1 (LSD 51% = 6) on stubble-incorporation plots and 50 kg N ha−1 on stubble-retention plots. Tillage had little effect on burnt plots. Delaying N application from sowing until stem elongation increased average fertilizer N uptake from 26 to 39 kg N ha−1 (LSD 5% = 6), but reduced soil N uptake from 50 to 37 kg N ha− (LSD 5% = 10). Immobilization and leaching did not vary greatly between treatments and approximately one-third of the fertilizer was immobilized. Less than 1% of the fertilizer was found below a depth of 300 mm. Incorporating 9 t ha−1 of rice stubble 13 days before wheat sowing reduced net apparent mineralization of native soil N from 37 to 3 kg ha−1 between tillering and maturity. It also increased apparent denitrification of fertilizer N from an average 34 to 53 kg N ha−1 (LSD 5% = 6). N loss occurred over several months, suggesting that denitrification was maintained by continued release of metabolizable carbohydrate from the decaying rice stubble. The results demonstrate that no-till systems increase crop yield and use of both fertilizer and soil N in intensive rice-based rotations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00709659
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  • 6
    Electronic Resource
    Electronic Resource
    Variation in nitrogen fixation among populations ofFrankia sp. andCeanothus sp. in actinorhizal association (1989)
    Springer
    Biology and fertility of soils 7 (1989), S. 269-274 
    Details
    ISSN: 1432-0789
    Keywords: Nitrogen fixation ; Frankia-Ceanothus spp. association ; Acetylene reduction assay (ARA) ; Microsymbiont population ; Nodules ; Actinomycetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wildland shrub improvement is needed for sound range and disturbed land revegetation practice. The possibility of selecting superior N2-fixingFrankia-Ceanothus spp. actinorhizal associations was examined. Greenhouse tests were used to expose various soil-borne microsymbiont andCeanothus sp. population accessions in reciprocal combination. The acetylene reduction rate was used as a measure of N2-fixation capacity. There was no significant interaction between host and microsymbiont regardless of source for all variables measured. The acetylene reduction rate, nodule number and mass, plant biomass, and root: shoot ratio were significantly different among soil sources. The acetylene reduction rate was not significantly different amongCeanothus sp. accessions. Neither was it strongly correlated with other variables. It was concluded that the N2-fixation rate is more a function ofFrankia sp. than the hostCeanothus sp. in actinorhizal associations. It appears possible to select soil sources with superior N2-fixing microsymbiont populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00709660
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  • 7
    Electronic Resource
    Electronic Resource
    Nitrogen fixation in the lichen Lobaria pulmonaria in elevated atmospheric carbon dioxide (1989)
    Springer
    Oecologia 79 (1989), S. 566-568 
    Details
    ISSN: 1432-1939
    Keywords: Carbon dioxide ; Lichen ; Lobaria ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thalli of Lobaria pulmonaria (L.) Hoffm., a nitrogen-fixing epiphyte common in mesic temperate forests, were collected in a Douglas-fir (Pseudotsuga menziesii Franco) forest near Corvallis, Oregon, and maintained for 20 to 40 days in controlled-environment chambers with atmospheric CO2 concentrations of 374 and 700 μll-1. Nitrogenase activity, which was assayed by the acetylene reduction method, was approximately doubled in the lichen maintained in elevated CO2. Increases in nitrogen fixation by lichens may be an important part of the integrated ecosystem response to rising CO2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378677
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  • 8
    Electronic Resource
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    Energy transduction efficiencies in nitrogenous oxide respirations of Azospirillum brasilense Sp7 (1989)
    Springer
    Archives of microbiology 151 (1989), S. 445-453 
    Details
    ISSN: 1432-072X
    Keywords: Denitrification ; Growth yield measurements ; Nitrate respiration ; Nitrogen fixation ; Proton translocations in respirations ; Azospirillum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For Azospirillum brasilense Sp7, the energy transformation efficiencies were measured in anaerobic respirations with either nitrate, nitrite or nitrous oxide as respiratory electron acceptors by determining the maximal molar growth yields and the H+-translocations using the oxidant pulse method. In continuous cultures grown with malate limiting, the maximal molar growth yields (Y s max -values) were essentially the same with O2 or N2O but were 1/3 and 2/3 lower with NO 2 - or NO 3 - , respectively, as respiratory electron acceptors. Both the maximal molar growth yields and the maintenance energy coefficients were surprisingly high when Azospirillum was grown with nitrite as the sole electron acceptor and source for N-assimilation. Growth under N2-fixing conditions drastically reduced the Y s max -values in the N2O and O2-respiring cells. In the H+-translocation measurements, the $$\vec H^ + $$ /oxidant ratios were 5.6 for O2→H2O, 2.5–2.8 for NO 3 - →NO 2 - , 2.2 for NO 2 - →N2O and 3.1 for N2O→N2 respirations when the cells were preincubated with valinomycin and K+. All the values were enhanced when the experiments were performed with valinomycin plus methyltriphenylphosphonium (=TPMP+) cation. The uncoupler carbonyl cyanide-m-chlorophenyl-hydrazone diminished the H+-excretion indicating that this translocation was due to vectorial flow across the membrane. In the absence of any ionophore, nitrate and nitrite respirations were accompanied by a H+-uptake $$(NO_3^ - \to N_2 = - 2.9 \vec H^ + /NO_3^ - and NO_2^ - \to N_2 = - 2.5 \vec H^ + /NO_2^ - )$$ . Any significant H+-translocation could not be detected in N2O- and O2-respirations under these conditions. It is concluded that nitrate reduction proceeds inside the cytoplasmic membrane, whereas nitrite is reduced extramembraneously. The data are not conclusive for the location of nitrous oxide reductase. The maximal molar growth yield determinations and the absence of any H+-uptake in untreated cells indicate a cytoplasmic orientation of the enzyme similar to the terminal cytochrome oxidase of respiration. The low H+-extrusion values for N2O-respiration compared to O2-respiration in cells treated with valinomycin plus TPMP+ are, however, not in accord with such an interpretation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00416605
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  • 9
    Electronic Resource
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    Cloning of a DNA region from Bradyrhizobium japonicum encoding pleiotropic functions in heme metabolism and respiration (1989)
    Springer
    Archives of microbiology 151 (1989), S. 203-212 
    Details
    ISSN: 1432-072X
    Keywords: Bradyrhizobium ; Gene cloning ; Heme ; Marker exchange mutagenesis ; Nitrogen fixation ; Respiration ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Random and site-directed Tn5-induced mutagenesis of Bradyrhizobium japonicum yielded two mutations, one in strain 2960 and the other in strain 2606::Tn5-20, which mapped close to each other but in separate genes. The corresponding wild-type genes were cloned, and their approximate location on the cloned DNA was determined. Mutant 2960 was Fix- and formed green nodules on soybean, whereas strain 2606::Tn5-20 had ca. 4% of wild-type Fix activity and formed white nodules. Cytochrome oxidase assays (Nadi tests) showed a negative reaction with both mutants, indicating a functional deficiency of cytochrome c or its terminal oxidase or both. However, the mutants grew well under aerobic conditions on minimal media with different carbon sources. Furthermore, mutant 2960 had a reduced activity in hydrogen uptake, was unable to grow anaerobically with nitrate as the terminal electron acceptor and 2960-infected soybean nodules contained little, if any, functional leghemoglobin. Southern blot analysis showed that a B. japonicum heme biosynthesis mutant [strain LO505: O'Brian MR, Kirshbom PM, Maier RJ (1987) Proc Natl Acad Sci USA 84: 8390–8393] had its mutation close to the Tn5 insertion site of our mutant 2606::Tn5-20. This finding, combined with the observed phenotypes, suggested that the genes affected in mutants 2960 and 2606::Tn5-20 were involved in some steps of heme biosynthesis thus explaining the pleiotropic respiratory deficiencies of the mutants. Similar to strain LO505, the mutant 2606::Tn5-20 (but not 2960) was defective in the activity of protoporphyrinogen IX oxidase which catalyzes the penultimate step in the heme biosynthesis pathway. This suggests that one of the two cloned genes may code for this enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413131
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  • 10
    Electronic Resource
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    Deletion analysis of the nitrogen fixation regulatory gene, nifL of Klebsiella pneumoniae (1989)
    Springer
    Archives of microbiology 151 (1989), S. 180-182 
    Details
    ISSN: 1432-072X
    Keywords: Klebsiella pneumoniae ; Nitrogen fixation ; nifL ; Regulation ; Oxygen control ; Nitrogen control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of in-frame deletions have been constructed in the Klebsiella pneumoniae regulatory gene nifL. The effects of each nifL mutation on NifA-mediated expression from the nifH promoter of K. pneumoniae have then been assessed with respect to both nitrogen and oxygen control. These experiments indicate that, in contrast to the situation with the homologous regulatory proteins NtrB and NtrC, NifA activity is not impaired in the absence of NifL. We conclude that the only function of NifL is to inactivate NifA in response to an increase in the nitrogen or oxygen status of the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414436
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  • 11
    Electronic Resource
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    Location of two isoenzymes of NADH-dependent glutamate synthase in root nodules of Phaseolus vulgaris L. (1989)
    Springer
    Planta 179 (1989), S. 441-447 
    Details
    ISSN: 1432-2048
    Keywords: Glutamate synthase ; Glutamine synthetase ; Nitrogen fixation ; Phaseolus (glutamate synthase) ; Plastid (glutamate synthase) ; Root nodule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The two isoenzymes of NADH-dependent glutamate synthase (NADH-GOGAT; EC 1.4.1.14), previously identified in root nodules of Phaseolus vulgaris L., have both been shown to be located in root-nodule plastids. The nodule specific NADH-GOGAT II accounts for the majority of the activity in root nodules, and is present almost exclusively in the central tissue of the nodule. However about 20% of NADH-GOGAT I activity is present in the nodule cortex, at about the same specific activity as this isoenzyme is found in the central tissue. Glutamine synthetase (GS; EC 6.3.1.2) occurs predominantly as the γ polypeptide in the central tissue, whereas in the cortex, the enzyme is represented mainly by the β polypeptide. Over 90% of both GS and NADH-GOGAT activities are located in the central tissue of the nodule and GS activity exceeds NADH-GOGAT activity by about twofold in this region. Using the above information, a model for the subcellular location and stoichiometry of nitrogen metabolism in the central tissue of P. vulgaris root nodules is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397583
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  • 12
    Electronic Resource
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    Efficient production of haploid wheat (Triticum aestivum) through crosses between Japanese wheat and maize (Zea mays) (1989)
    Springer
    Plant cell reports 8 (1989), S. 263-266 
    Details
    ISSN: 1432-203X
    Keywords: Wheat ; Maize ; Intergeneric cross-Embryo culture ; Haploid production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four Japanese wheat varieties, three crossable and one non-crossable with Hordeum bulbosum, were pollinated with maize pollen of 5 genotypes. By the application of 2,4-dichlorophenoxyacetic acid after pollination, embryos kept developing on wheat plants until 14 days after pollination. The frequency of embryo formation was significantly different among the maize genotypes, varying from 18.0% to 31.9%, but not among the wheat varieties. By bagging spikes with flag leaves the frequency of embryo formation was increased by about 7%. Ten- to twelve-day-old embryos gave higher frequencies of plant formation (83.6%) than 14-day-old embryos(50.0%). All 6 regenerated plants investigated cytologically were found to be haploid. Twelve of the 14 colchicine-treated plants produced florets setting seeds. The overall efficiency of our procedure is considered to be higher than that reported by Laurie and Bennett (1988).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274125
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  • 13
    Electronic Resource
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    Identification of new mitochondrial genome organizations in wheat plants regenerated from somatic tissue cultures (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 169-175 
    Details
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA ; Chondriome variability ; In vitro culture ; Plant regeneration ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants have been regenerated from short-and long-term in vitro somatic tissue cultures made from immature embryos of the hexaploid wheat cultivar “Chinese Spring”. The mitochondrial genome organization of each regenerated plantlet was studied, after one selfing, by probing Sal I-restricted total DNA with cloned Sal I fragments of wheat mitochondrial DNA derived from a segment of the genome, which displays marked structural changes in response to in vitro culture. Short-term in vitro cultures give rise to regenerated plants whose mitochondrial genome organization is either close to that of the parental cultivar or to that of embryogenic callus cultures, except for a single plant which has an organization resembling that of short-term non-embryogenic cultures. In contrast, all but one of the plants regenerated from long-term cultures exhibited a mitochondrial genome organization similar to that of long-term nonembryogenic cultures. In addition, extra labelled bands were detected in some of the regenerated plants with two of the probes used. These results emphasize the importance of the duration of the in vitro step preceding the regeneration process: the longer it is, the higher the probability is of obtaining mitochondrial DNA variability in regenerated plants. Furthermore, since increasing the duration of the in vitro stetp results in the production of regenerated plants with a mitochondrial genome organization resembling that of non-embryogenic tissue cultures, the question is thus raised as to whether regeneration from long-term cultures is suitable for use in plant breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266182
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  • 14
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    Biochemical and cytological characterization of wheat/Aegilops ventricosa addition and transfer lines carrying chromosome 4MV (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 184-188 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Powdery mildew resistance ; Biochemical markers ; Addition and transfer lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene encoding a variant of alcohol dehydrogenase, Adh-μ, has been found to be associated with the chromosome of the Mv genome which is present in type 9 wheat/Aegilops ventricosa addition line, to which the genes for protein CM-4 and for a phosphatase variant, Aph-v, had been previously assigned. Transfer line H-93-33, which has 42 chromosomes and has been derived from the cross (Triticum turgidum x Ae. ventricosa) x T. aestivum, carries genes encoding all three biochemical markers. Linkage between these genes has been demonstrated by analysis of individual kernels of the F2 (H-93-33 x T. aestivum cv. “Almatense” H-10-15). A study of the hybrids of line H-93-33 with T. aestivum H-10-15 and with the 4DS ditelosomic line has confirmed that, as suspected, the linkage group corresponds to chromosome 4Mv from Ae. ventricosa. Additionally, it has been found that the previously reported resistance of line H-93-33 to powdery mildew (Erysiphe graminis) is also linked to the biochemical markers; this indicates that either the gene responsible for it is different from that in lines H-93-8 and H-93-35, or that a translocation between two different Mv chromosomes has occurred in line H-93-33.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266184
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  • 15
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    Genetic variability and advance under four selection procedures in wheat pedigree breeding programme (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 516-520 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Single seed descent (SSD) ; Single plant selection (SPS) ; Bulk population (BP) ; Mechanical mass selection (MMS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four methods of generation advance (SPS, SSD, BP and MMS) were compared in F3 and F4 generations. In the F3 generation, the SPS and SSD methods of generation advance proved superior to the BP and MMS methods for grain yield per plant and for at least one of the yield component traits. The F3 SSD population did not differ significantly from the F3 SPS for any of the traits. However, the F3 SSD population retained more range and cv for different traits than with other methods of generation advance. F4 progenies derived from F3 SSD population were significantly superior for grain yield than lines derived from the other three F3 populations. The MMS method of generation advance proved useful for increasing the 1,000-grain weight for which initial selection was made.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274272
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  • 16
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    Comparative performance of bread wheat and hexaploid triticale cytoplasms (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 685-688 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Triticale ; Cytoplasmic effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirteen wheat-like advanced-generation triticale x wheat derivatives, having tetraploid wheat cytoplasm from triticale, were reciprocally crossed with three improved bread wheats, and the resulting F1s were evaluated for determining the comparative performance of the bread wheat and triticale cytoplasms for different traits. Significant reciprocal differences in the mean performance were observed for days to heading, days to maturity, spikes/plant, flag-leaf area, peduncle length, plant height, spike length, grains/spike, 1,000-grain weight, grain yield and grain protein content, and most of them were in favour of hexaploid wheat cytoplasm. However, this superiority of the hexaploid cytoplasm was not universal for a particular trait, implying that the differences in the performance of the evaluated reciprocal crosses depended not solely on the cytoplasmic background, but also on the interplay of the specific genotype with the cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261245
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  • 17
    Electronic Resource
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    Is Nor region variability in wheat invariably caused by tissue culture? (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 809-814 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Ribosomal DNA ; Tissue culture ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a previous study we observed extensive Nor region variability in tissue-culture derived plants of only one out of three tested wheat cultivars. This finding prompted us to further question whether or not this variability was invariably caused by in vitro culture. In the present study, the upper halves of spikes from four source plants of the inbred cultivar ‘ND7532’ were removed 12 days after anthesis. The immature embryos from these halves were cultured and regenerated into plants. The lower halves of the same spikes were retained on the plants to obtain mature caryopses. DNA was extracted from seedlings, cut with TaqI endonuclease, run on agarose gels, and the respective Southern blots were probed with the plasmid pTA71 to reveal the Nor region patterns. The sexual progeny of regenerants from three out of four source plants derived from the immature embryos provided Nor region patterns which were exactly identical to the patterns obtained from seedlings which germinated from the caryopses matured on the respective source spikes. The regenerants from the fourth source plant provided variable Nor region patterns. Analyses of the Nor region patterns of 21 individual seedlings germinated from caryopses of this source plant showed that 18 had a three-fragment pattern (consisting of 3.0, 2.7 and 1.9 kb fragments) while three seedlings lacked one (2.7 of 1.9 kb) fragment. Furthermore, the next sexual progeny of the regenerants which had a three-fragment pattern further segregated into three- and two-fragment patterns.These results, in conjunction with previous reports on Nor region variability among tissue-culture derived plants, suggest that this variability is not invariably related to in vitro culture.
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    URL: http://dx.doi.org/10.1007/BF00268331
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  • 18
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    Use of tissue culture to bypass wheat hybrid necrosis (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 57-60 
    Details
    ISSN: 1432-2242
    Keywords: Hybrid necrosis ; Wheat ; Immature embryoculture ; Young ear culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hybrid necrosis in wheat is a barrier to gene transfer in wheat breeding practice. It is based on two complementary genes, Ne1 and Ne2. Recovery mutants (Re1, Re2 and Re3) which can grow to maturity were recovered from immature embryo cultures of necrotic hybrids between T. aestivum and T. durum. Cytological observation demonstrated that Re1 had 34 chromosomes instead of 35. This indicated that one of the chromosomes carrying the Ne genes was lost. Genetic study suggested that for Re1, the lost chromosome was chromosome 5B of the durum parental line. Re mutants are male sterile but can be maintained through a young ear culture method. Re mutants could be successfully pollinated by either parental line and the BC1 progeny is partially fertile. Re mutants were repeatedly induced in about 1% of the regenerated plants from immature embryo culture. This technique provides a practical way to bypass hybrid necrosis.
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    URL: http://dx.doi.org/10.1007/BF00299753
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  • 19
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    Inheritance of somatic embryogenesis and organ regeneration from immature embryo cultures of winter wheat (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 137-142 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Diallel analysis ; Somatic embryogenesis ; Plant regeneration ; In vitro culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Diallel analyses of F1 and reciprocal crosses among five winter wheat lines show that additive, non-additive, and cytoplasmic genetic effects were significant in the genetic control of somatic embryogenesis, shoot, and root induction frequencies as well as in numbers of somatic embryos, shoots, and roots. However, additive genetic effect appears to be most important since, in most cases a larger portion of the cross variation was accounted for by general combining ability. The results suggest that somatic embryogenesis and organ regeneration in winter wheat can be improved through genetic manipulation. Due to the presence of maternal effects, it may be critical to use a suitable genotype as a female parent in a selection program.
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    URL: http://dx.doi.org/10.1007/BF00299767
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  • 20
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    Control of tissue culture response in wheat (Triticum aestivum L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 783-787 
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    ISSN: 1432-2242
    Keywords: Tissue culture ; Callus ; Wheat ; Ditelosomics ; Nullisomic-tetrasomics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ability of immature embryos of wheat (Triticum aestivum L.) to respond to tissue culture has been shown to involve the group 2 chromosomes. The available group 2 ditelosomic and nullisomic-tetrasomic lines of ‘Chinese Spring’ wheat were used to determine the chromosome arm location and chromosome dosage effect associated with the expression of tissue culture response (TCR). Significant differences were found between the aneuploid lines and the euploid control for the expression of both regenerable callus formation and callus growth rate. A model is proposed suggesting that a major TCR gene is located on 2DL and that 2AL and 2BS possess minor TCR genes. Furthermore, a major regulatory gene controlling the expression of TCR genes may be located on chromosome 2BL.
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    URL: http://dx.doi.org/10.1007/BF00266658
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  • 21
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    Evidence for cytoplasmic control of in vitro microspore embryogenesis in the anther culture of wheat (Triticum aestivum L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 867-872 
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    ISSN: 1432-2242
    Keywords: Wheat ; Cytoplasm ; Anther culture ; Microspore embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anthers were cultured from two sets of seven lines of hexaploid wheat (Triticum aestivum L.) with different cytoplasms, the euplasmic nucleus donors, ‘Siete Cerros 66’ and ‘Penjamo 62’, as well as their six alloplasmic lines derived from wild relative species of the genera Triticum and Aegilops. Significant cytoplasmic and nuclear effects but no cytoplasmic-nuclear interaction were found for embryogenic anther response, with the best performance of ‘Penjamo 62’ in Ae. kotschyi cytoplasm. Plant regeneration was not affected significantly by the cytoplasmic background of the lines cultured. The possible genetic implications of the observed cytoplasmic and nuclear influences on the in vitro haploid induction of wheat are discussed.
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    URL: http://dx.doi.org/10.1007/BF00266673
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  • 22
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    Genetic analysis of anther culture response in wheat using aneuploid, chromosome substitution and translocation lines (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 7-11 
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    ISSN: 1432-2242
    Keywords: Wheat ; Anther culture ; Regeneration ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Marked effects of genotype on wheat anther culture response have been observed. Genetic factors have been recognised to be one of the major contributors to in vitro responses of cultured wheat tissues. In wheat anther culture, embryo induction, plant regeneration and albina/green ratio have been determined to be heritable traits. Using Chinese Spring (CS) monosomic 1D, single chromosome substitution lines of chromosome 5B or chromosome arm 5BL from Chinese Spring into six varieties, and F1 hybrids heterozygous for the 1B chromosome structure (1BL-1BS/1BL-1RS), the anther culture response was studied: genes on CS1D chromosome and 5BL chromosome arm increases the embryo frequency; gene(s) involved in regeneration ability are located on the 1RS chromosome arm; a gene increasing albina frequency is located on Chinese Spring 5B chromosome. Our results support the fact that without gametic selection, a differential development occurred from the particular classes of microspores carrying genes for higher regeneration ability. Moreover, in some crosses, a few genes with major effects were involved in determination of anther culture response.
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    URL: http://dx.doi.org/10.1007/BF00292308
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    A modified pedigree method of selection (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 173-176 
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    ISSN: 1432-2242
    Keywords: Wheat ; Tiller index ; Competitive ability ; Interplant competition ; Modified pedigree method of selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A dismal 81% failure of newly bulked lines against checks was observed in Indian wheat material evolved by the pedigree method. This is considered to be the consequence of selection in space planting in early generations followed by yield tests in a competitive environment which did not allow any scope of selection for competitive ability. Thus, most of the homozygous lines, which were until now raised and carried forward under space planting, failed to compete with the checks for yield in close planting. It is, therefore, necessary to modify the method to combine both the competitive ability and identity of selected plants. The proposed modified procedure involves raising the population under spaced planting as well as under drilling from F2 onwards. Evaluation for tiller index marks an important step. On the basis of tiller index, single plants are isolated from spaced populations. Steps of the modified procedure are outlined in detail. The modified method, which economises on breeders' workload and other physical inputs, besides having other advantages, can also be used for certain other grain crops. Preliminary results based on the modified method during the past two seasons have indicated its utility.
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    URL: http://dx.doi.org/10.1007/BF00288795
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    The chromosomal location of a third set of malate dehydrogenase loci, Mdh-3, in wheat, barley and related species (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 349-352 
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    ISSN: 1432-2242
    Keywords: Wheat ; Malate dehydrogenase ; Isozymes ; Triticeae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A third set of malate dehydrogenase loci have been identified and located on the short arms of homoeologous group 5 chromosomes in wheat. Allelic differences have been found at each of the three Mdh-3 loci. However, Mdh-D3 appears to be least variable, with a second allele found only in Sears' ‘Synthetic’ among a survey of 42 varieties. Homoeoloci were identified on chromosome 7 (5H) of Hordeum vulgare, the short arm of 5E in Agropyron elongatum and 5U in Aegilops umbellulata.
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    URL: http://dx.doi.org/10.1007/BF00265295
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    Induced genetic variability in Rhizobium leguminosarum for nitrogen fixation parameters in Vicia faba L. (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 433-435 
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    ISSN: 1432-2242
    Keywords: Rhizobium ; Irradiation ; Nitrogen fixation ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Sumary The objective of this work was to know the behaviour and variability of Rhizobium leguminosarum after irradiation. The induced variation was tested under greenhouse conditions on the variety JV 3 of broad beans (Vicia faba) in six replications. Induced genetic variabilty was observed for strain, parent and mutant versus parent. Out of 24 irradiated strains, strain 93-32 performed better with a greater number of nodules and higher dry weight of nodules per plant and biological yield. Environment played an important role in the expression of characters observed. High heritability and genetic advance of these traits indicated that the nitrogen fixation ability of Rhizobium can easily be improved by selection.
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    URL: http://dx.doi.org/10.1007/BF00265308
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    Production and cytogenetics of hybrids of Triticum aestivum x Leymus innovatus (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 436-444 
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    ISSN: 1432-2242
    Keywords: Intergeneric hybrids ; Wheat ; Leymus ; Chromosome pairing ; Meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hybrid plants were obtained between Triticum aestivum (2n=6x=42, AABBDD) and Leymus innovatus (2n=4x=28, JJNN) at a frequency varying from 0.4% to 1.2% of the pollinated florets. Improvement of the embryo culture medium resulted in a higher frequency of embryo rescue. Eight of ten hybrids had the expected chromosome number of 35 (ABDJN). Meiotic analysis indicated that there was no homology between the genomes of the two species. Two hybrids had only 28 chromosomes. Comparison of chromosome pairing between the two types of hybrids suggested that Leymus innovatus carries genes that affect chromosome pairing and behavior. The relatively high occurrence of spontaneous doubling in the meiocytes of these hybrids may indicate that backcrossing of the hybrids to wheat should be possible, although frequent chromosome irregularities observed in the meiocytes of the hybrids may decrease the probability of success of this step, which is essential to the process of gene transfer from L. innovatus to wheat.
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    RFLP-based genetic maps of wheat homoeologous group 7 chromosomes (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 495-504 
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    ISSN: 1432-2242
    Keywords: Wheat ; Genetic mapping ; RFLPs ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism (RFLP) mapping was attempted using 18 cDNA clones, 14 anonymous and 4 of known function, which had been shown to have homologous DNA sequences on the group 7 chromosomes of wheat. The loci identified by these probes have been mapped on one or more chromosomes in this homoeologous group using linkage data derived from various F2, random inbred, doubled haploid and single chromosome recombinant populations. The maps also include three isozyme loci, five disease resistance loci, two anthocyanin pigment loci and a vernalisation response locus. The mapping data have been used to determine the extent of map co-linearity over the A, B and D genomes, the degree of RFLP variability in the three genomes and the relative efficiency of various restriction enzymes in detecting RFLPs in wheat. The strategy for future mapping in wheat, particularly the use of “alien” genomes or segments, such as that from Aegilops ventricosa used here, is discussed.
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    URL: http://dx.doi.org/10.1007/BF00290833
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    Chromosomal control of the aminopeptidases of wheat and its close relatives (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 657-664 
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    ISSN: 1432-2242
    Keywords: Aminopeptidase ; Wheat ; Isoelectric focussing ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isoelectric focussing was used to separate the isozymes of aminopeptidase of wheat and its relatives. Three distinct homoeoallelic sets of genes have been shown to be present. AMP-1, controlled by genes on the long arms of group 6, has previously been described, but two new systems, AMP-2 (group 4) and AMP-3 (group 7) are described here. The three systems are distinguished by their electrophoretic characteristics, by their genetic control and by their substrate specificity. Intervarietal, interspecific and intergeneric polymorphism has been observed at most of the loci. A further set of isozymes, AMP-4, was detected but the chromosomal control of these could not be determined.
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    URL: http://dx.doi.org/10.1007/BF00262561
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    Nuclear genes affecting albinism in wheat (Triticum aestivum L.) anther culture (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 879-883 
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    ISSN: 1432-2242
    Keywords: Wheat ; Anther culture ; Androgenetic responsiveness ; Albinism ; Genetic control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inheritance of the ability to respond in wheat anther culture was studied from 6×2 reciprocal crosses between six varieties with high and two varieties with low capacity for green plant formation and their parents, replicated in two environments. Effects of genotypes dominated embryo formation and percentages of green plants, accounting for 78.4% and 85.4% of total variation, respectively, while smaller genetic effects were indicated for regeneration. Nuclear genes could explain almost all the genotype effects in this material. Embryo formation showed heterosis over high parent for 5 of the 12 hybrids, while percentages of green plants from the hybrids were intermediate to the parents. General Combining Ability (GCA) could explain 78.8% of the variation for embryo formation among the hybrids, whereas differences in percentage of green plants were dominated by Specific Combining Ability (SCA), accounting for 67.9% of hybrid variation. A positive correlation (r=0.81**) was observed between the genetic capacity for regeneration and green plant formation. Analysis of covariance indicated that effects causing GCA for green plant formation were mainly responsible for this correlation. A regression model with two parallel lines divided the six parent lines with high green plant formation into three groups with respect to their reactions with the two testers. The results are discussed with regard to possible involvement of two sets of nuclear genes affecting the percentage of green plants obtained in wheat anther culture: one set consisting of mainly additive effects affecting green plant percentage through an initial effect on regeneration ability, and another set of two or a few more major genes with dominance or epistatic effects uncorrelated with regeneration.
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    Role of metal ions in negative regulation of nitrogen fixation by the nifL gene product from Klebsiella pneumoniae (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 484-491 
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    ISSN: 1617-4623
    Keywords: Nitrogen fixation ; nifL ; Repression ; Metal ions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ability of the Klebsiella pneumoniae nifL gene product to antagonise NIFA mediated transcriptional activation from the nifH promoter in vivo was inhibited either by metal deprivation, or by the presence of the iron chelators EDDA or Desferal in the growth medium. This inhibition of the repressive activity of NIFL was reversed by the addition of ferrous or manganous ions to the medium but was unaffected by other transition metals. The dependence on metal ions for NIFL activity was observed when NIFL was overexpressed and when cultures were exposed to oxygen or high levels of fixed nitrogen. Immunochemical evidence suggests that NIFL and NIFA associate to form a functional protein complex. Metal ions are apparently not required for the formation of this complex.
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    URL: http://dx.doi.org/10.1007/BF00334394
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    TheKlebsiella pneumoniae PII protein (glnB gene product) is not absolutely required for nitrogen regulation and is not involved in NifL-mediatednif gene regulation (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 474-480 
    Details
    ISSN: 1617-4623
    Keywords: glnB ; Klebsiella pneumoniae ; Nitrogen control ; Glutamine synthetase ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The role of theKlebsiella pneumoniae PII protein (encoded byglnB) in nitrogen regulation has been studied using two classes ofglnB mutants. In Class I mutants PII appears not to be uridylylated in nitrogen-limiting conditions and in Class II mutants PII is not synthesised. The effects of these mutations on expression from nitrogen-regulated promoters indicate that PII is not absolutely required for nitrogen control. Furthermore the uridylylated form of PII(PII-UMP) plays a significant role in the response to changes in nitrogen status by counteracting the effect of PII on NtrB-mediated dephosphorylation of NtrC. PII is not involved in thenif-specific response to changes in nitrogen status mediated by NifL.
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    URL: http://dx.doi.org/10.1007/BF02464920
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    Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 81-90 
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    ISSN: 1617-4623
    Keywords: LMW glutenin ; Seed storage protein ; Molecular evolution ; Tissue-specific expression ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The isolation and characterisation by DNA sequencing of a low molecular weight (LMW) glutenin gene from wheat is described. The deduced protein contains a signal peptide, a central repetitive region rich in proline and glutamine and N and C terminal non-repetitive domains, similar to other prolamins. A detailed comparison of the C terminal domain of 20 prolamin genes enabled us to divide them into 4 families. The LMW glutenin family is distinct from the α, β-and γ-gliadin families of wheat and is closest to the B hordein genes of barley. This and other comparisons were also used to assess the pattern of genetic variation among prolamin sequences and to provide a molecular basis for the interpretation of prolamin size polymorphism. The 5′ flanking fragment of the isolated gene was previously shown to direct endosperm-specific expression of a reporter gene in transgenic tobacco. Evidence is provided that the isolated gene is also active in wheat and its transcription initiation site was determined. Features of the gene which may be relevant to its activity are discussed.
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    URL: http://dx.doi.org/10.1007/BF00332234
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    DNA sequence and genetic analysis of the Rhodobacter capsulatus nifENX gene region: Homology between NifX and NifB suggests involvement of NifX in processing of the iron-molybdenum cofactor (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 353-363 
    Details
    ISSN: 1617-4623
    Keywords: Rhodobacter capsulatus ; Nitrogen fixation ; DNA sequence analysis ; nifE, nifN, nifX genes ; Protein comparisons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rhodobacter capsulatus genes homologous to Klebsiella pneumoniae nifE, nifN and nifX were identified by DNA sequence analysis of a 4282 bp fragment of nif region A. Four open reading frames coding for a 51188 (NifE), a 49459 (NifN), a 17459 (NifX) and a 17472 (ORF4) dalton protein were detected. A typical NifA activated consensus promoter and two imperfect putative NifA binding sites were located in the 377 bp sequence in front of the nifE coding region. Comparison of the deduced amino acid sequences of R. capsulatus NifE and NifN revealed homologies not only to analogous gene products of other organisms but also to the α and β subunits of the nitrogenase iron-molybdenum protein. In addition, the R. capsulatus nifE and nifN proteins shared considerable homology with each other. The map position of nifX downstream of nifEN corresponded in R. capsulatus and K. pneumoniae and the deduced molecular weights of both proteins were nearly identical. Nevertheless, R. capsulatus NifX was more related to the C-terminal end of NifY from K. pneumoniae than to NifX. A small domain of approximately 33 amino acid residues showing the highest degree of homology between NifY and NifX was also present in all nifB proteins analyzed so far. This homology indicated an evolutionary relationship of nifX, nifY and nifB and also suggested that NifX and NifY might play a role in maturation and/or stability of the iron-molybdenum cofactor. The open reading rame (ORF4) downstream of nifX in R. capsulatus is also present in Azotobacter vinelandii but not in K. pneumoniae. Interposon-induced insertion and deletion mutants proved that nifE and nifN were necessary for nitrogen fixation in R. capsulatus. In contrast, no essential role could be demonstrated for nifX and ORF4 whereas at least one gene downstream of ORF4 appeared to be important for nitrogen fixation.
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    Ultrastructural characteristics of the host-symbiont interface in nitrogen-fixing peanut nodules (1989)
    Springer
    Protoplasma 150 (1989), S. 19-26 
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    ISSN: 1615-6102
    Keywords: Nitrogen fixation ; Peanut ; Root nodules ; Dense body ; Microbody ; Oleosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nitrogen-fixing peanut root nodules are characterized by their unique structural organization, distinct from other legume nodules. The focus of this study has been in and around the hostsymbiont interface, where the bacterioid and the host cell surface (peribacteroid membrane envelope) interact during symbiosis. The infected nodule cells have revealed the presence of lipid bodies (oleosomes) in intimate association with the peribacteroid membrane, which encloses the large spherical bacteroids with a relatively narrow peribacteroid space. Electron dense structures, referred to as dense bodies have been found attached to the bacteroid outer membranes at the host-symbiont interface. The dense bodies are osmiophilic, amorphous and 3,3′-diaminobenzidine positive. The isolated intact bacteroids with dense bodies attached to their cell wall showed significant catalase activity. Many microbodies showing DAB-positive reaction have been found in the host cytoplasm, associated closely with the peribacteroid membrane. These ultrastructural and cytochemical characteristics of peanut root nodules suggest that lipids are utilized during symbiosis and the dense bodies and microbodies may be involved in the catabolic process.
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    Ultrastructure of the nitrogen-fixing, filamentous, nonheterocystous cyanobacterium,Plectonema boryanum (1989)
    Springer
    Protoplasma 152 (1989), S. 130-135 
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    ISSN: 1615-6102
    Keywords: Plectonema boryanum ; Cyanobacteria ; Ultrastructure ; Nitrogen fixation ; Nitrogen starvation ; Immunogold localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructure of fructose-supplemented and unsupplemented nitrogen-fixing (fix +) and nonfixing (fix −)Plectonema boryanum UTEX 581 cells was examined by transmission electron microscopy. The most prominent structural differences included the arrangement and morphology of the thylakoids and alterations in the appearance of the interthylakoidal spaces. These ultrastructural differences, together with other observations such as glycogen content and presence of nitrogenase (using acetylene reduction assay and immunogold localization), readily distinguished nonfixingP. boryanum from nitrogen-fixing cells.
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    TheRhizobium-legume symbiosis Two methods to discriminate between nodules and other root-derived structures (1989)
    Springer
    Protoplasma 149 (1989), S. 82-88 
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    ISSN: 1615-6102
    Keywords: Legumes ; Nitrogen fixation ; Nodulation ; Rhizobium ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two methods have been developed in order to discriminate between lateral roots, nodules and root-derived structures which exhibit both root and nodule histological features and which can develop on legumes inoculated with certainRhizobium mutants. The first method, known as the “clearing method”, allows the observation by light microscopy of cleared undissected root-structures. The second, known as the “slicing method”, is a complementary technique which provides a greater degree of structural information concerning such structures. The two methods have proved invaluable in defining unequivocally the nature of the interaction between a rhizobial strain and a legume host.
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    Characterization and nucleotide sequence of a novel gene fixW upstream of the fixABC operon in Rhizobium leguminosarum (1989)
    Springer
    Molecular genetics and genomics 218 (1989), S. 536-544 
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    ISSN: 1617-4623
    Keywords: Rhizobium leguminosarum ; Nitrogen fixation ; nif/fix genes ; Escherichia coli minicells ; Transcription regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary On the Rhizobium leguminosarum PRE sym plasmid, fixABC and a novel gene fixW were identified upstream of the regulatory gene nifA. The molecular masses of FixABC, 29, 44 and 50 kDa respectively, were estimated by polyacrylamide gel electrophoresis (PAGE) and of FixW, 25 kDa, by PAGE and nucleotide sequencing. Hybridization studies using bacteroid mRNA as a probe showed that fixABC is one operon which can be transcribed independently of fixW. Nucleotide sequencing revealed that both fixW and fixA are preceded by a nif consensus promoter. The fixA promoter partly overlaps the 3′-terminal coding region of fixW, indicating that readthrough from fixW into fixA is possible. Two open reading frames, ORF71 and ORF79, precede fixW and form one operon with fixW. ORF71 contains sequences homologous to the fixA promoter and 5′-terminal coding region. One more duplication of fixA sequences was detected, also located within the sym plasmid nif/fix clusters. One duplication of fixW sequences was found. No fixW homologue could be found in other nitrogen fixing organisms except in a number of R. leguminosarum strains.
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    URL: http://dx.doi.org/10.1007/BF00332421
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    Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum (1989)
    Springer
    Molecular genetics and genomics 218 (1989), S. 340-347 
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    ISSN: 1617-4623
    Keywords: Nitrogen fixation ; draT ; draG ; TTG initiation codon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nitrogen fixation activity in the photosynthetic bacterium Rhodospirillum rubrum is controlled by the reversible ADP-ribosylation of the dinitrogenase reductase component of the nitrogenase enzyme complex. This report describes the cloning and characterization of the genes encoding the ADP-ribosyltransferase (draT) and the ADP-ribosylglycohydrolase (draG) involved in this regulation. These genes are shown to be contiguous on the R. rubrum chromosome and highly linked to the nifHDK genes. Sequence analysis revealed the use of TTG as the initiation codon of the draT gene as well as a potential open reading frame immediately downstream of draG. The mono-ADP-ribosylation system in R. rubrum is the first in which both the target protein and modifying enzymes as well as their structural genes have been isolated, making it the model system of choice for analysis of this post-translational regulatory mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331287
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  • 39
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    The DNA sequence of the Rhodobacter capsulatus ntrA, ntrB and ntrC gene analogues required for nitrogen fixation (1989)
    Springer
    Molecular genetics and genomics 215 (1989), S. 507-516 
    Details
    ISSN: 1617-4623
    Keywords: Nitrogen fixation ; Regulation ; Rhodobacter capsulatus ; Gene sequences ; Transcription factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have determined the DNA sequence for the genes nifR1, nifR2 and nifR4 in the photosynthetic bacterium Rhodobacter capsulatus. These genes regulate transcription of the nifHDK operon and so limit the expression of nitrogen fixation activity to periods of low environmental concentrations of both oxygen and fixed nitrogen. The sequences of these three genes are similar to components of the ntr regulation system in Escherichia coli and Klebsiella pneumoniae. The two-component regulatory system of ntrB and ntrC in E. coli is represented by nifR2 and nifR1 in R. capsulatus and nifR4 in R. capsulatus is the equivalent of the E. coli ntr-related sigma factor ntrA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427050
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  • 40
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    Rice yields as influenced by Azolla N2 fixation and urea N-fertilization (1989)
    Springer
    Plant and soil 114 (1989), S. 63-68 
    Details
    ISSN: 1573-5036
    Keywords: Azolla pinnata ; Nitrogen fixation ; N yield ; Oryza sativa ; Urea-N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Application of 0, 30, 60, 90 and 120 kg N ha−1 of urea (U) in split doses with (and without)Azolla pinnata, R. Brown was studied for three consecutive seasons under planted field condition. Fresh weight (FW), acetylene reduction activity (ARA) and N yield of Azolla were found to be maximum 14 days after inoculation (DAI). Among the different treatments, maximum Azolla growth was recorded in no N control. The FW, ARA and N yield of Azolla were inhibited increasingly with the increase in N levels. Irrespective of season, FW and N yield of Azolla were inhibited only a small extent with 90 kg N ha−1 U, beyond which the inhibition was pronounced. ARA was inhibited only slightly up to 60 kg N ha−1 of U. Grain yield and crop N uptake of rice increased significantly up to 90 kg N ha−1 of U (alone or in combination with Azolla) in the dry seasons (variety IR 36) and up to 60 kg N ha−1 U in the wet season (variety CR 1018).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02203082
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