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  • Immunohistochemistry  (40)
  • tissue culture  (32)
  • Springer  (72)
  • American Chemical Society
  • American Meteorological Society
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • Springer Nature
  • 1985-1989  (72)
  • 1975-1979
  • 1970-1974
  • 1960-1964
  • 1950-1954
  • 1988  (72)
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  • 1985-1989  (72)
  • 1975-1979
  • 1970-1974
  • 1960-1964
  • 1950-1954
Year
  • 1
    Electronic Resource
    Electronic Resource
    A new device to open tissue culture flasks (1988)
    Springer
    Methods in cell science 11 (1988), S. 31-33 
    Details
    ISSN: 1573-0603
    Keywords: tissue culture ; flask opening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A device has been designed to open tissue culture flasks, which is quick and permits easy access for cell cloning, the removal of complete cell sheets, and staining for microscopy. The brass blade used melts the plastic rather than burning, thus reducing the level of potentially toxic fumes produced by some other methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404412
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  • 2
    Electronic Resource
    Electronic Resource
    Ferulate esters of higher fatty alcohols and allelopathy inKalanchöe daigremontiana (1988)
    Springer
    Journal of chemical ecology 14 (1988), S. 589-603 
    Details
    ISSN: 1573-1561
    Keywords: Kalanchöe daigremontiana ; triacontanol ; ferulate esters ; ferulic acid ; tissue culture ; allelopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ferulate esters of normal C22–C30 alcohols were found in the root extract ofKalanchöe daigremontiana and the freen-C30 alcohol, triacontanol, was found on the leaves. Ferulic acid was isolated from the vermiculite in which plants were grown. Whole plant and tissue culture experiments were done to investigate the role of ferulic acid as an allelochemical and of triacontanol as a plant growth regulator inK. daigremontiana and other bioassay systems. No positive growth responses to triacontanol were observed, but inhibitation of growth response of plantlets by ferulic acid was seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01013909
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric tomato somatic hybrid plants (1988)
    Springer
    Plant molecular biology 11 (1988), S. 139-145 
    Details
    ISSN: 1573-5028
    Keywords: Lycopersicon esculentum Mill. ; ribosomal DNA ; Solanum lycopersicoides Dun. ; somatic hybrid ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment polymorphisms were used to identify and quantify the nuclear contributions from each parent to somatic hybrid plants between tomato (Lycopersicon esculentum Mill.) cv. Sub-Arctic Maxi and Solanum lycopersicoides Dun. Three single-copy clones, 2–13, 2–17, and 3–288, and a clone for the 45s ribosomal RNA, pHA2, all mapped to chromosome 2 of tomato, were used in analysis of 47 somatic hybrids. The amount of hybridizing probe for each parental band was quantified by densitometry of the autoradiograph film. Analyses with the three single-copy clones indicated that there were more than two S. lycopersicoides copies in most somatic hybrid plants. For at least one somatic hybrid there was a loss of one tomato copy. No evidence was found for more than two copies donated from tomato or loss of a copy from S. lycopersicoides. Most of the observed variation in copy number of the single-copy clones was consistent with chromosomal changes occurring in the suspension cells from which S. lycopersicoides parental protoplasts were derived. The number of copies of rDNA derived from each parent varied independently of the number of copies of single-copy clones from each parent. Changes in the copy number of rDNA occurred in both tomato and S. lycopersicoides genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015666
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  • 4
    Electronic Resource
    Electronic Resource
    Distributional pattern of oxytocin- and vasopressin-immunoreactivity in the neurohypophysis of the Djungarian hamster (Phodopus sungorus) (1988)
    Springer
    Cell & tissue research 253 (1988), S. 677-681 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Hypothalamo-neurohypophyseal system ; Vasopressin ; Oxytocin ; Immunohistochemistry ; Phodopus sungorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The topography of oxytocin (OT)- and vasopressin (VP)-containing axons of the hypothalamo-neurohypophyseal system was studied in the neurohypophysis of the Djungarian hamster (Phodopus sungorus) by means of immunohistochemistry. Compared with other mammalian species, the neurohypophysis of Phodopus shows some peculiarities. Accumulations of OT-immunoreactivity around the distal vessels of the primary portal plexus can be observed in the distal median eminence and neural stem. This staining pattern indicates that OT is secreted into portal blood. In the neural lobe, OT- and VP-immunopositive fibers terminate in different areas. The vast majority of the OT-containing axons is distributed in the dorsal part of the neural lobe. In contrast, VP-containing axons are mainly found in the centre of the neural lobe up to the pars intermedia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219760
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  • 5
    Electronic Resource
    Electronic Resource
    Immunoreactivity to vasoactive intestinal polypeptide (VIP) and thyreotropin-releasing hormone (TRH) in hypothalamic neurons of the domesticated pigeon (Columba livia). Alterations following lactation and exposure to cold (1988)
    Springer
    Cell & tissue research 251 (1988), S. 485-494 
    Details
    ISSN: 1432-0878
    Keywords: Immunohistochemistry ; Light microscopy ; Neuroendocrine regulation ; Neuropeptides ; Columba livia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of VIP- and TRH-immunoreactivity in neurons and processes within the hypothalamus of the pigeon was investigated with light-microscopic immunocytochemical techniques. Most of the VIP-containing neurons are concentrated in the middle and caudal parts of the hypothalamus, with the greatest concentration of perikarya occurring in the medial and lateral part of the ventromedial hypothalamic nucleus and the infundibular nucleus. These cells give rise to axons that seem to extend into the median eminence. An extensive network of VIP-immunoreactive fibers and varicosities occupy the external layer of the median eminence. The majority of TRH-containing neurons is found in the anterior hypothalamus with the greatest concentration of cells in the magnocellular preoptic, medial preoptic, suprachiasmatic and paraventricular nuclei. TRH-immunoreactive fibers and varicosities form a dense arborization in the external layer of the median eminence. Lactation seems to induce substantial changes in VIP as well as in TRH-immunostaining in the median eminence and other hypothalamic regions as compared to control, sexually active animals. Furthermore, TRH-immunoreactivity decreased in the median eminence following 60-min exposure to cold. These results suggest that VIP- and TRH-containing pathways in the pigeon hypothalamus are involved in the mediation of neuroendocrine responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215858
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of GABAergic perikarya and terminals in the centers of the higher auditory pathway of the chicken (1988)
    Springer
    Cell & tissue research 252 (1988), S. 99-106 
    Details
    ISSN: 1432-0878
    Keywords: γ-Aminobutyric acid (GABA) ; Glutamic acid decarboxylase ; Immunohistochemistry ; Auditory system ; Chicken (White Leghorn)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of presumed GABAergic neurons and axon terminals in nuclei of the higher auditory pathway of the chicken was investigated by immunocytochemical methods employing antisera to the rate-limiting enzyme of GABA synthesis, glutamic acid decarboxylase, and to GABA. In the mesencephalic auditory center (MLD) about 20% of the cells reveal immunoreactivity. In contrast, the thalamic relay station nucleus ovoidalis is devoid of immunostained somata. This nucleus contains a high density of punctate immunoreactive structures presumed to be GABAergic axon terminals. In the auditory forebrain center field L and the auditory portions of the hyperstriatum ventrale, up to 8% of the cells were immunopositive. These neurons were significantly smaller than estimated from measurements of the overall cell population in these nuclei. From the two-dimensional arrangement of immunopositive neurons it is suggested that the GABAergic system in the avian auditory telencephalon consists of two separate groups of neurons: one subgroup mediating local inhibitory interactions, the other responsible for lateral inhibition between different frequency representations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213830
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  • 7
    Electronic Resource
    Electronic Resource
    Immunohistochemical and ultrastructural localisation of peptide-containing nerves and myocardial cells in the human atrial appendage (1988)
    Springer
    Cell & tissue research 254 (1988), S. 155-166 
    Details
    ISSN: 1432-0878
    Keywords: Neuronal markers ; Neuropeptides ; Immunohistochemistry ; Heart innervation ; Atrial natriuretic peptide (ANP) ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation and myocardial cells of the human atrial appendage were investigated by means of immunocytochemical and ultrastructural techniques using both tissue sections and whole mount preparations. A dense innervation of the myocardium, blood vessels and endocardium was revealed with antisera to general neuronal (protein gene product 9.5 and synaptophysin) and Schwann cell markers (S-100). The majority of nerve fibres possessed neuropeptide Y immunoreactivity and were found associated with myocardial cells, around small arteries and arterioles at the adventitial-medial border and forming a plexus in the endocardium. Subpopulations of nerve fibres displayed immunoreactivity for vasoactive intestinal polypeptide, somatostatin, substance P and calcitonin gene-related peptide. In whole-mount preparations of endocardium, substance P and calcitonin gene-related peptide immunoreactivities were found to coexist in the same varicose nerve terminals. Ultrastructural studies revealed the presence of numerous varicose terminals associated with myocardial, vascular smooth muscle and endothelial cells. Neuropeptide Y immunoreactivity was localised to large electron-dense secretory vesicles in nerve terminals which also contained numerous small vesicles. Atrial natriuretic peptide immunoreactivity occurred exclusively in myocardial cells where it was localised to large secretory vesicles. The human atrial appendage comprises a neuroendocrine complex of peptidecontaining nerves and myocardial cells producing ANP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220029
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  • 8
    Electronic Resource
    Electronic Resource
    GABA-like immunoreactivity of identified interneurons in the flight system of the locust, Locusta migratoria (1988)
    Springer
    Cell & tissue research 254 (1988), S. 331-340 
    Details
    ISSN: 1432-0878
    Keywords: GABA ; Interneurons ; Flight ; Immunohistochemistry ; Locusta migratoria (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The transmitter content of identified inhibitory interneurons in the flight system of the locust, Locusta migratoria, has been characterized using antibodies raised against protein-conjugated gamma aminobutyric acid. Identified flight neurons were filled with the fluorescent dye, Lucifer Yellow. Serial sections of dye-filled neurons were incubated with an antibody to gamma aminobutyric acid which was subsequently tagged with a fluorescent marker. Excitatory motoneurons to wing muscles and 13 flight interneurons (3 excitatory, 7 inhibitory, and 3 with unknown synaptic effect) were examined. Neither the moto-neurons nor any of the 3 excitatory interneurons contained immunoreactive material. Six of the 7 inhibitory interneurons did contain immunoreactive material. All the neurons which contained immunoreactive material and whose synaptic effect is known were inhibitory. We conclude that most of the inhibitory flight interneurons which have been described use gamma aminobutyric acid as their transmitter. Interestingly, at least 1 set of interneurons known to be inhibitory does not use gamma aminobutyric acid. We predict that the 2 interneurons which do contain immunoreactive material and whose synaptic effect is not yet known will be found to have inhibitory roles in the operation of the flight circuitry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225805
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  • 9
    Electronic Resource
    Electronic Resource
    Somatostatin-like immunoreactivity in non-pyramidal neurons of the human entorhinal region (1988)
    Springer
    Cell & tissue research 254 (1988), S. 361-367 
    Details
    ISSN: 1432-0878
    Keywords: Entorhinal cortex ; Non-pyramidal neurons ; Interstitial neurons ; Somatostatin ; Immunohistochemistry ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of somatostatin-immunoreactive cells and processes throughout the human entorhinal region and subjacent white matter was examined either by the unlabelled antibody-enzyme method or by the avidin-biotin method. The brain slices were obtained at autopsy with a short post-mortem delay. The majority of somatostatin immunoreactive nerve cells was found in the inner principal layer and subjacent white matter. In addition, individually scattered immunoreactive neurons were observed in both the outer principal layer and lamina dissecans. The immunoreactive perikarya varied in shape and ranged in size from 10 to 30 μm. Without exception the neurons could be classified as belonging to the group of non-pyramidal neurons. Each neuron gave rise to a few thick dendrites and a thin axon with a beaded appearance. In the adult human brain, the pattern formed by lipofuscin granules deposited in the nerve cells can be considered characteristic for the type of the neuron. Therefore, immunoreactive perikarya were documented, destained of chromogen and restained to demonstrate lipofuscin pigment and basophilic substance. It became evident from these studies that the previously immunoreactive cells were characterized by a large rounded and eccentrically located nucleus, sparse basophilic substance and, in most cases, a lack of lipofuscin granules. A few of the immunoreactive cells were laden with coarse pigment granules. The findings permit classification of entorhinal somatostatin-immunoreactive neurons as either non-pigmented or pigment-laden non-pyramidal neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225808
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  • 10
    Electronic Resource
    Electronic Resource
    Localization of 28 kDa calbindin in human odontoblasts (1988)
    Springer
    Cell & tissue research 254 (1988), S. 341-346 
    Details
    ISSN: 1432-0878
    Keywords: Odontoblast ; Calbindin ; Immunohistochemistry ; Electron microscopy ; Teeth ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The presence of 28 kDa calbindin in human odontoblasts was studied by use of specific antibodies raised against chick duodenal 28 kDa calbindin, in immunofluorescence, immuno-peroxidase, and electron-microscopic labelling experiments. The calbindin-like protein was detected mainly in the cytoplasm of odontoblast cell bodies, in their processes and occasionally in their nuclei. Correspondingly, at the ultrastructural level, immunoreactive material was associated with the cytosol, microfilaments and cilia. These findings suggest that human odontoblasts express a 28 kDa vitamin D-dependent calcium-binding protein, unlike those of rats and mice in which ameloblasts are the only cells immunoreactive for the protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225806
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