ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Column liquid chromatography  (54)
  • Springer  (54)
  • American Chemical Society (ACS)
  • National Academy of Sciences
  • 1985-1989  (54)
  • 1980-1984
  • 1987  (54)
Collection
Keywords
Publisher
  • Springer  (54)
  • American Chemical Society (ACS)
  • National Academy of Sciences
  • Wiley-Blackwell  (1)
Years
  • 1985-1989  (54)
  • 1980-1984
Year
  • 1
    Electronic Resource
    Electronic Resource
    An efficient procedure for extraction and determination of steroids in the tissue of laboratory animals (1987)
    Springer
    Chromatographia 23 (1987), S. 796-802 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Steroids ; Rat tissue ; Purification by extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A high-performance liquid chromatographic method for analysis of steroids in the tissue of laboratory animals is described. A two-stage solid extraction procedure, utilizing Sep-pak C18 and graphitized carbon black, gives a purified product. Steroids in this extract were separated by multistep gradient elution with a reversedphase liquid chromatography system, using acetonitrilewater as solvent. Several columns with different geometry and phase loading were investigated. Optimum resolution was obtained with a medium covered and spherical particle. The applicability of this method was successfully demonstrated in the analysis of the testis and adrenal gland of rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02311402
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    HPLC of aminoacids on silica coated with ammonium tungstophosphate (1987)
    Springer
    Chromatographia 23 (1987), S. 814-817 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Thin layer chromatography ; Ammonium tungstophosphate ; Aminoacids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The chromatographic behaviour of aminoacids on columns of silica coated with different percentages of ammonium tungstophosphate at several buffer phosphate concentrations is reported. The stationary phase shows a high selectivity towards aminobutyric acids and methyl derivatives of tryptophan due to the distance between the carboxylic group and the −NH 3 + group involved in the cation-exchange process and/or to the steric hindrance by a methyl substituent. A relationship between HPLC and HPTLC data has been verified and applied to the prediction of the separation of six aminoacids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02311405
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Limits of determination and calibration in HPLC (1987)
    Springer
    Chromatographia 23 (1987), S. 934-938 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Calibration ; Confidence interval ; Limit of determination ; Limit of calibration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary In HPLC calibration the expressions lowest calibration limit and determination limit are defined in statistical terms. The lowest calibration limit is the minimum mass in the measured series of calibration points. It is calculated from the confidence interval of the inverse of the calibration function as the lowest mass limit that may be differentiated from zero mass with a preset probability of error. If the calculated lowest calibration limit is lower than the actual data, points at lower concentration may be measured. The determination limit is the smallest concentration of an analysis that is differentiated from the concentration zero or an apparent blind value in the calibration curve with a given probability of error. Using two different UV-detectors (variable wavelength and photodiode-array) the lowest calibration limit is experimentally evaluated and compared with specific data for the detectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261474
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Sub-nanogram detection of dihydroartemisinin after chemical derivatization with diacetyldihydrofluorescein followed by high-performance liquid chromatography and UV absorption (1987)
    Springer
    Chromatographia 23 (1987), S. 112-114 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pre-column derivatization ; Ultraviolet detector sensitivity ; Dihydroartemisinin ; Diacetyldihydrofluorescein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The use of diacetyldihydrofluorescein (DADF) for derivatization of dihydroartemisinin (dihydroqinghaosu, DHQHS) is proposed. The reaction between DHQHS and this reagent in the presence of 4-dimethylaminopyridine (DMAP) and N,N′-dicyclohexylcarbodiimide (DCC) was complete in 8 hours at room temperature giving about 80 per cent theoretical yield. The derivative showed intense UV absorption, thus providing a sensitivity of 0.1 nanogram by UV detection after column separation. The influences of the ratio of the reagents, reaction temperature, chromatographic conditions and the extent of detection linearity were investigated. The reaction gave consistent results and chromatographic separation was not affected by an excess of the reagent or side products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02312884
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    HPLC in photokinetics: Determination of reaction mechanism and photochemical quantum yields of (E)-1-phenylpropene (1987)
    Springer
    Chromatographia 23 (1987), S. 395-400 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Photokinetic data ; Reaction mechanism ; (E)-1-phenylpropene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A combined method of high-performance liquid chromatography and UV-absorption spectroscopy is described for use in dynamic systems. It provides a way of obtaining detailed mechanistic information as well as quantitative data (rate constants, quantum yields), even though neither mechanism nor photoproducts are known. The kinetic treatment of the photoreaction of (E)-1-phenylpropene was successful, showing a photo-isomerization and a parallel step. Information on this additional step is obtained by using HPLC to observe the changes in the concentrations of the reactants' selectivity. The necessity of process control is demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02311813
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Determination of particulate fatty acids as P-bromophenacyl- or phenylphenacylesters using HPLC (1987)
    Springer
    Chromatographia 23 (1987), S. 419-422 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fatty acid esters ; Particulate material in seawater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A method for the determination of fatty acids of the molecular weight range of propionic to erucic acid is reported. The acids are converted to either p-bromophenacyl-or p-phenylphenacylesters and the esters are separated by HPLC and detected by UV-absorption. Twentytwo esters, including saturated, monoolefinic and polyunsaturated ones were separated within one HPLC-run. The detection limit is about 5 pmol per fatty acid. The application of the method to particulate material is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02311817
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    New polymer coated anion-exchange HPLC-phases: Immobilization of poly (2-hydroxy, 3N-ethylenediamino) butadiene on silica and alumina (1987)
    Springer
    Chromatographia 23 (1987), S. 435-441 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Anion-exchange stationary phases ; Polymer coating ; Cross-linking
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary PHEB=POLY(2-hydroxy, 3N-ethylenediamino)butadiene has been synthesized. This unsaturated pre-polymer can be immobilized on different, preferably inorganic supports of suitable porosity such as silica or alumina by cross-linking. Weak anion-exchange phase are obtained. Such phases do not have the disadvantages of organic polymeric phases, regarding pressure stability and swelling by solvent influence. The ion-exchange capacity and the retentivity of IE-separations can deliberately be varied via the thickness of the cross-linked polymer layer. Alumina can also be coated with PHEB applying the same cross-linking procedure. Such phases exhibit an exceptional chemical stability even when operated with mobile phases at very high pH-values. Separations of excellent selectivity are achieved for inorganic anions, as well as with mixtures of aliphatic and aromatic free acids also including hydroxy- (mono- or poly)carboxylic acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02311821
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Application of the electrostatic interaction concept for the study of the retention behaviour of peropyrene-type polycyclic aromatic hydrocarbons in reversed-phase liquid chromatography (1987)
    Springer
    Chromatographia 23 (1987), S. 473-476 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed-phase systems ; Stationary phase-solute interactions ; Polycyclic aromatics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Capacity factors of peropyrene-type polycyclic aromatic hydrocarbons were measured using 15 different liquid chromatographic reversed phase systems. On the basis of the retention data the electric interaction indices have been calculated. Application of these indices for structure-retention studies on peropyrene-type polycyclic aromatic hydrocarbons is demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02309413
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Separation of gonadal steroid epimers using high performance liquid chromatography (1987)
    Springer
    Chromatographia 23 (1987), S. 31-32 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Gonadal steroids ; Epimers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Chromatographic separation of biologically active epimeric steroids was carried out using a combination of normal and reversed phases. Testosterone (17β-OH) was separated from its 17α-OH epimer epitestosterone using a normal phase silica column whereas their reduced 5α-metabolites were separated on a reversed phase system. The separation of other gonadal steroids including the epimers 20α- and 20β-hydroxypregn-4-en-3-one is also discussed. The technique is particularly useful for separating mixtures of naturally occurring steroid epimers prior to radioimmunoassay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02310414
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Separation of chlorophylls and their degradation products in marine phytoplankton by reversed-phase high-performance liquid chromatography (1987)
    Springer
    Chromatographia 23 (1987), S. 26-30 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ammonium acetate buffer eluent ; Chlorophylls and degradation products ; Marine phytoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An RP-HPLC procedure which separates chlorophylls and their degradation products is described. By employing an amonium acetate buffered mobile phase and a linear gradient elution, complex mixtures of chloroand phaeopigments are separated in less than 30 minutes. The method has been applied to the control of chlorophyll enzymatic degradation in cultures of two algal species, and has also been successfully used in the analysis of algal xanthophylls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02310413
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...