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  • Immunocytochemistry  (42)
  • Springer  (42)
  • American Meteorological Society
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • Public Library of Science
  • Springer Nature
  • 2015-2019
  • 1985-1989  (42)
  • 1975-1979
  • 1970-1974
  • 1960-1964
  • 1950-1954
  • 1987  (42)
Sammlung
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Verlag/Herausgeber
  • Springer  (42)
  • American Meteorological Society
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • Public Library of Science
    • +
Erscheinungszeitraum
  • 2015-2019
  • 1985-1989  (42)
  • 1975-1979
  • 1970-1974
  • 1960-1964
    • +
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum (1987)
    Springer
    Archives of microbiology 147 (1987), S. 190-194 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Methanobacterium thermoautotrophicum ; Methyl-CoM reductase ; Immunocytochemistry ; Colloidal gold ; Energy conservation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cells of Methanobacterium thermoautotrophicum were fixed with glutaraldehyde, sectioned and labeled with antibodies against the β subunit of component C (=methyl-CoM reductase) of methyl-CoM reductase system and with colloidal gold-labeled protein A. It was found that the gold particles were located predominantly in the vicinity of the cytoplasmic membrane, when the cells were grown under conditions where methyl-CoM reductase was not overproduced. This finding confirms the recent data obtained with Methanococcus voltae showing via the same immunocytochemical localization technique that in this organism methyl-CoM reductase is membrane associated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00415283
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Localization of nitrogenase in vesicles of Frankia sp. Cc1.17 by immunogoldlabelling on ultrathin cryosections (1987)
    Springer
    Archives of microbiology 146 (1987), S. 327-331 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Actinomycetes ; Nitrogen fixation ; Symbiosis ; Immunocytochemistry ; Ultracryotomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Immunogoldlabelling on ultrathin cryosections of Frankia sp. Cc1.17 showed specific labelling of nitrogenase in the spherical cells called vesicles. No label was found in the hyphae in any cells grown on a medium with combined nitrogen, nor in those to which no specific antiserum was added. Similar results were obtained with cultures grown under high (20%) and low (2%) oxygen tension in the gas phase.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00410930
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  • 3
    Digitale Medien
    Digitale Medien
    Localization of ornithine decarboxylase in the chick embryo during organogenesis (1987)
    Springer
    Cell & tissue research 247 (1987), S. 75-84 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Ornithine decarboxylase ; Chick embryo ; Organogenesis A ; Autoradiography ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The localization of ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis and thus in cell growth, was determined in the 4.5-day-old chick embryo, using two independent methods of analysis. ODC protein was identified by indirect immunofluorescence with a monospecific ODC antibody, and catalytically active ODC was identified by autoradiography with α-(5-3H) difluoromethylornithine. Both methods revealed a basically similar distribution of ODC within the embryo. Among the organs, the brain exhibited the highest ODC levels. ODC levels were also high in spinal cord, mesonephric tubules and heart. Similar levels, but confined to limited areas, were found in liver tissue, head mesenchyme, and the oral and pharyngeal regions. Organs that exhibited high ODC levels are all engaged in rapid growth, as well as in extensive tissue remodeling and differentiation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216549
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  • 4
    Digitale Medien
    Digitale Medien
    Immunocytochemical and ultrastructural evidence for a neurophysinergic innervation of the subcommissural organ of the snake Natrix maura (1987)
    Springer
    Cell & tissue research 248 (1987), S. 473-478 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Innervation ; Neu rophysins ; Mesotocin ; Immunocytochemistry ; Ultrastructure ; Snake, Natrix maura
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The subcommissural organ (SCO) of the snake Natrix maura was studied by use of the immunoperoxidase procedure. Primary antisera against bovine neurophysins (Nps I + II, OXY-Np), oxytocin (OXY), mesotocin (MST), arginine-vasotocin (AVT), somatostatin (SOM), β-endorphin (END) and bovine Reissner's fiber were used. A conventional ultrastructural study, with special emphasis on the nerve fibers present in the SCO, was also performed. Nerve fibers containing immunoreactive OXY-Np and MST were seen to reach the SCO. The staining of adjacent sections with the anti-Reissner's fiber serum showed that the OXY-Np- and MST-immunoreactive fibers were distributed among the cell bodies and processes of the ependymal secretory cells. No fibers containing immunoreactive OXY, AVT, SOM or END were found in the SCO. The ultrastructural analysis revealed in the SCO the presence of nerve fibers filled with electron-dense granules, 170–210 nm in diameter. Although a direct apposition between these fibers and the SCO cells was frequently seen, no synaptic differentiations were identified. Structures identical to the Herring bodies (found in the neurohypophysis) were seen in the SCO.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218215
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  • 5
    Digitale Medien
    Digitale Medien
    Immunocytochemical markers revealing retinal and pineal but not hypothalamic photoreceptor systems in the Japanese quail (1987)
    Springer
    Cell & tissue research 248 (1987), S. 161-167 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Retina ; Pineal organ ; Hypothalamus ; Opsin ; α-Transducin ; Interstitial retinol-binding protein (IRBP) ; Immunocytochemistry ; Japanese quail
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The retinal proteins opsin,α-transducin, S-antigen and interstitial retinol-binding protein (IRBP) are essential for the processes of vision. By use of immunocyto-chemistry we have employed antibodies directed against these “photoreceptor proteins” in an attempt to identify the photoreceptor systems (retina, pineal and deep brain) of the Japanese quail. Opsin immunostaining was identified within many outer (basal portion) and inner segments of retinal photoreceptor cells and limited numbers of photoreceptor perikarya. Opsin immunostaining was also demonstrated in limited numbers of pinealocytes with all parts of these cells being immunoreactive. These results differ from previous observations. In contrast to the results obtained with the antibody against opsin, S-antigen andα-transducin immunostaining was seen throughout the entire outer segments and many photoreceptor perikarya of the retina. In the pineal organ immunostaining was seen in numerous pinealocytes in all follicles. These results conform to previous findings in birds. In addition, IRBP has been demonstrated for the first time in the avian retina and pineal organ. These findings underline the structural and functional similarities between the retina and pineal organ and provide additional support for a photoreceptive role of the avian pineal. No specific staining was detected in any other region of the brain in the Japanese quail; the hypothalamic photoreceptors of birds remain unidentified.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01239977
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Insulin-, glucagonand somatostatin-like immunoreactivity in the endocrine pancreas of the lungfish,Neoceratodus forsteri (1987)
    Springer
    Cell & tissue research 248 (1987), S. 181-185 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Endocrine pancreas ; Insulin ; Glucagon ; Somatostatin ; Immunocytochemistry ; Neoceratodus forsteri (Australian lungfish)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The endocrine pancreas of the Australian lungfish,Neoceratodus forsteri, was investigated immunocytochemically for the presence of polypeptide hormone-producing cells. Three cell types were identified, namely insulin-, glucagon-, and somatostatin-immunoreactive elements. The insulin cells are confined solely to the center of the islets. Glucagon and somatostatin cells are distributed peripherally around the central mass of the insulin cells. Isolated cells or clusters of glucagon and somatostatin cells are also dispersed within the exocrine parenchyma. The immunoreactive cell types are compared with those staining with standard histological procedures. The spatial relationships of the different cell populations are examined.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01239979
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  • 7
    Digitale Medien
    Digitale Medien
    Nerve growth factor-like immunoreactivities in rodent salivary glands and testis (1987)
    Springer
    Cell & tissue research 248 (1987), S. 275-286 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Nerve growth factor ; Salivary glands ; Antibodies ; Immunocytochemistry ; Affinity purification ; Specificity tests ; Testis ; Mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A series of polyclonal affinity-purified antibodies against mouse submandibular-gland nerve growth factor (NGF) are described. Using the submandibular gland of the male mouse and indirect immunofluorescence, the specificity and sensitivity of affinity-purified immunoglobulins and various other fractions from the immunized animals have been tested. It will be shown that affinity-purification schemes, including pre-purification of protein A-fractionated immunoglobulins to remove antibodies that bind to unrelated hydrophilic and hydrophobic proteins, significantly enhance the signal-to-noise ratio and specificity of the antibodies. The antibodies effectively detect NGF-like immunoreactivity in both fresh and fixed glandular tissue. Optimal fixation procedures are described. Fluorescence intensities are linearly correlated to log antibody concentration. By use of the best antibody fractions and optimal fixation protocols, the distribution of NGF-like immunoreactivity is described in eight different salivary glands (rat and mouse, male and female, submandibular and sublingual glands). In addition to the well-known large numbers of immunoreactive cells in the submandibular gland of the male mouse, immunoreactive cells were found in the sublingual gland of male mice and in the submandibular and sublingual glands of female mice. One antibody revealed a weak specific fluorescence also in the submandibular gland of the male mouse. In a survey of genital organs of male mice, one antibody revealed fluorescence in the germ cell line. We conclude that several polyclonal affinity-purified antibodies have been characterized that show a strong NGF-dependent binding to the secretory granules of tubular cells in the submandibular gland of male mice. These antibodies should make it possible to locate endogenous and perturbed NGF levels immunocytochemically, e.g., in the peripheral and central nervous system, where NGF concentrations may be several orders of magnitude lower than in the salivary glands.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218194
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  • 8
    Digitale Medien
    Digitale Medien
    An FMRFamide antiserum differentiates between populations of antigens in the brain and retrocerebral complex of the locust, Schistocerca gregaria (1987)
    Springer
    Cell & tissue research 250 (1987), S. 93-99 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Nervous system ; Immunocytochemistry ; FMRFamide ; Bovine pancreatic polypeptide ; Insects ; Schistocerca gregaria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distribution of FMRFamide-irmunoreactive cell bodies in the brain and retrocerebral complex of the locust, Schistocerca gregaria, is described. Most of the immunoreactive cell bodies are found in the pars intercerebralis and in the optic lobes. Many, but not all, of the cell bodies also react with an antiserum raised against bovine pancreatic polypeptide, but this antiserum also reveals another population of cells that stain selectively with this antiserum. In addition to the cell bodies, numerous immunoreactive processes are revealed by both antisera in neuropilar regions of the brain. The results of blocking experiments suggest that a differential distribution of three locust antigens can be determined from the examination of alternate serial sections stained with the two antisera used.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214659
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  • 9
    Digitale Medien
    Digitale Medien
    Immunocytochemical study of the GABAergic innervation of the mouse pituitary by use of antibodies against gamma-aminobutyric acid (GABA) (1987)
    Springer
    Cell & tissue research 247 (1987), S. 33-40 
    Details
    ISSN: 1432-0878
    Schlagwort(e): GABA (gamma-aminobutyric acid) ; Pituitary ; Neuroendocrine regulation ; Immunocytochemistry ; Mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The GABAergic innervation of the mouse pituitary, including the median eminence, was studied at light microscopic and ultrastructural levels by use of a pre-embedding immunocytochemical technique with antibodies directed against GABA. In the median eminence, a high density of GABA-immunoreactive fibers was found in the external layer where the GABAergic varicosities were frequently observed surrounding the blood vessels of the primary capillary plexus. In the internal and subependymal layers, only few fibers were immunoreactive. The intense labeling of the external layer was observed in the entire rostro-caudal extent of the median eminence. In the pituitary proper, a dense network of GABA-immunoreactive fibers was revealed throughout the neural and intermediate lobes, entering via the hypophyseal stalk. The anterior and tuberal lobes were devoid of any immunoreactivity. The GABA-immunoreactive terminals were characterized in the median eminence, and in the intermediate and posterior lobes at the electron-microscopic level. They contained small clear vesicles, occasionally associated with dense-core vesicles or neurosecretory granules. In the intermediate lobe they were seen to be in contact with the glandular cells. In the posterior lobe and in the median eminence, GABA-immunoreactive terminals were frequently located in the vicinity of blood vessels. These results further support the concept of a role of GABA in the regulation of hypophyseal functions, via the portal blood for the anterior lobe, directly on the cells in the intermediate lobe, and via axo-axonic mechanisms in the median eminence and posterior lobe.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216544
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  • 10
    Digitale Medien
    Digitale Medien
    Fetal expression of muscle-specific isoactins in multiple organs of the Wistar-Kyoto rat (1987)
    Springer
    Cell & tissue research 250 (1987), S. 7-12 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; Isoactin ; Muscle ; Fetus ; Ontogeny ; Rat (Wistar-Kyoto)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Actin, a cytoskeletal and contractile protein, is expressed in six different isoforms that exhibit striking specificity. No studies have considered the muscle-specific actin expression in multiple organ systems in the intact fetus. Using a monoclonal antibody (B4) which reacts specifically with the isoactins of the smooth and skeletal muscle our immunohistochemical study examined whole fetal body sections to follow the development of actin expression throughout the last third of gestation in the Wistar-Kyoto rat. B4 staining was exclusively localized to muscle, confirming its high specificity and its usefulness for studying the ontogeny of muscle-specific isoactins. At 15 days of gestation, B4 staining was detected in the heart, the thoracic aorta and the skeletal muscle of the chest wall. The distribution and intensity of staining in the heart were initially higher than in the aorta or skeletal muscle and remained unchanged throughout the remainder of gestation, suggesting that the maturation of cardiac actin expression is well developed, although not fully completed before birth. Expression of muscle-specific actins in skeletal muscle was age-dependent and correlated with the maturational changes of muscle cell precursors. B4 staining in the fetal kidney was not apparent until day 20 of gestation and was localized to the inner cortical vessels. in association with the most mature nephrons, suggesting a centrifugal maturation of the intrarenal vasculature. The intensity of B4 staining in most tissues including bronchi, bowel, diaphragm, chest wall muscle and peripheral and pulmonary arteries increased by the end of gestation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214647
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