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  • Immunocytochemistry  (79)
  • Yeast  (58)
  • Springer  (137)
  • American Meteorological Society
  • Blackwell Publishing Ltd
  • Public Library of Science
  • Springer Nature
  • 2015-2019
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  • Springer  (137)
  • American Meteorological Society
  • Blackwell Publishing Ltd
  • Public Library of Science
  • Springer Nature
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  • 2015-2019
  • 2005-2009
  • 1985-1989  (137)
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  • 1
    Digitale Medien
    Digitale Medien
    Thermotolerant single cell protein production byKluyveromyces marxianus var.marxianus (1988)
    Springer
    Journal of industrial microbiology and biotechnology 3 (1988), S. 9-14 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Single cell protein ; Sucrose ; Yeast ; Thermotolerance ; Fermentation ; Kluyveromyces marxianus var.marxianus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Amino acid analyses were undertaken on single cell protein (SCP) produced by thermotolerant strains ofKluyveromyces marxianus var.marxianus grown on sugar cane molasses at 40°C. The maximum conversion of available sugars to biomass at 45°C was only 10.8% (g dry wt.·g−1 total sugars). The amino acid composition of the SCP did not differ markedly from that reported for other yeast species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01569436
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  • 2
    Digitale Medien
    Digitale Medien
    Immunological homologies between yeast ribosomal protein L2 and rat liver ribosomal proteins L4 and L24 (1988)
    Springer
    Current genetics 13 (1988), S. 235-239 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Ribosomal protein ; Immunological homology ; Yeast ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Polyclonal antibodies raised against ribosomal protein (r-protein) L2 of Schizosaccharomyces pombe were used to check for cross-reaktions with total r-proteins of rat liver. Using this procedure, the rat liver r-proteins, L4 and L24, were identified as being immunologically related to yeast L2. In addtional, homologies between rat liver L4 and L24 were detected. The possible implications for the regulation of r-protein synthesis are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00387769
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  • 3
    Digitale Medien
    Digitale Medien
    Plasmid associations with residual nuclear structures in Saccharomyces cerevisiae (1988)
    Springer
    Current genetics 13 (1988), S. 291-297 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast ; Nuclear matrix ; Plasmid stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Acentric yeast plasmids are mitotically unstable, apparently because they cannot freely diffuse after replicating and therefore are not included in the daughter nucleus. This behavior could result if plasmids remain attached to structural elements of the nucleus after replicating. Since DNA replication is believed to take place on the nuclear matrix, we tested whether there was a correlation between the mitotic stability of a given plasmid and the extent to which it was found associated with residual nuclear structures. Residual nuclei were prepared from yeast nuclei by extraction with either high salt, 2 M NaCl, or low salt, 10 mM lithium diiodosalicylate (LIS). Hybridization analysis was used to estimate the fraction of plasmid molecules remaining after nuclei were extracted. We examined the extent of matrix association of three ARSI plasmids, Trpl-RI circle (1.45 kb), YRp7 (5.7 kb) and pXBAT (45.1 kb) with mitotic loss rates ranging from 3–25%. In addition we examined the matrix binding of the endogenous 2 μm plasmid and the 2 μm-derived YEp 13 which is relatively stable in the presence of 2 μm and less stable in cir° strains. Among the ARS1 plasmids we observed a negative correlation between stability and matrix association, consistent with models in which binding to the nuclear matrix prevents passive segregation of ARS1 plasmid molecules. No such correlation was observed among the 2 μn plasmids. Among all plasmids examined there is a positive correlation between size and matrix association.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00424422
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  • 4
    Digitale Medien
    Digitale Medien
    Cloning of the orotidine 5′-phosphate decarboxylase (ODC) gene of Schwanniomyces occidentalis by complementation of the ura3 mutation in S. cerevisiae (1988)
    Springer
    Current genetics 13 (1988), S. 29-35 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast ; Cloning ; ODC ; Complementation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A DNA fragment containing the gene encoding orotidine 5′-phosphate decarboxylase (ODC) from the yeast, Schwanniomyces occidentalis (formerly castellii) has been isolated from a genomic library constructed in the S. cerevisiae expression vector, pYcDE8. A recombinant plasmid, p2-lA, containing a 2.47 kb insert was shown to complement the ura3-52 mutation of several strains of S. cerevisiae. This DNA insert was shown to be from Schwanniomyces occidentalis by Southern hybridization analysis. A restriction enzyme cleavage map of the insert has been derived and the ODC gene localized to a 1.1 kb region by deletion analysis. In addition, we have demonstrated that expression of ODC is not dependent on the ADHI promoter carried on pYcDE8. This is the first report of the cloning of a gene from a member of the genus Schwanniomyces.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00365753
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  • 5
    Digitale Medien
    Digitale Medien
    The glucose- and ethanol-dependent regulation of PDC1 from Saccharomyces cerevisiae are controlled by two distinct promoter regions (1988)
    Springer
    Current genetics 14 (1988), S. 337-344 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast ; Gene regulation ; Saccharomyces cerevisiae ; PDCI promoter
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A 870 by promoter fragment of the PDC1 gene that includes the carbon source dependent regulatory regions was investigated using 5′ and 3′ promoter deletions. The results indicate that glucose and ethanol regulation of PDC1 transcription are independently controlled by distinct cis-acting regions. The consensus sequence AAATCGATA may play a role in this regulation, while the sequence (ATCA)AACCT may be important in transcription initiation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00419991
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  • 6
    Digitale Medien
    Digitale Medien
    Multiple elements regulate expression of the cell cycle-regulated thymidylate synthase gene of Saccharomyces cerevisiae (1988)
    Springer
    Current genetics 14 (1988), S. 363-373 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Gene regulation ; Cell cycle ; Yeast
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Expression of the thymidylate synthase gene (TMPI) of Saccharomyces cerevisiae increases during the late G1 phase of the cell cycle. Using a series of gene fusions, which have placed the Escherichia coli lacZ gene under transcriptional and translational control of different portions of the TMPI gene, we have demonstrated the existence of three different regions which are important for expression. One of these regions, which was localized to within 270 base pairs of the translation start codon, is involved in the periodic expression of TMPI transcript. A second region, the deletion of which resulted in reduced levels of TMPI expression, is at least partially encoded by DNA sequences between 270 and 377 base pairs upstream of the translation start codon. A third region, located within the N-terminal 112 codons of the TMPI gene, apparently encodes information involved in a post-translational control mechanism.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00419994
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  • 7
    Digitale Medien
    Digitale Medien
    The cellular level of yeast ribosomal protein L25 is controlled principally by rapid degradation of excess protein (1986)
    Springer
    Current genetics 10 (1986), S. 733-739 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast ; Ribosome synthesis ; Regulation ; Ribosomal protein turnover
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary When the gene dosage for the primary rRNA-binding ribosomal protein L25 in yeast cells was raised about 50-fold, the level of mature L25 transcripts was found to increase almost proportionally. The plasmid-derived L25 transcripts were structurally indistinguishable from their genomic counterparts, freely entered polysomes in vivo and were fully translatable in a heterologous in vitro system. Nevertheless, pulse-labelling for periods varying from 3–20 min did not reveal a significant elevation of the intracellular level of L25 protein. When pulse-times were decreased to 10–45 s, however, we did detect a substantial over production of L25. We conclude that, despite the strong RNA-binding capacity of the protein, accumulation of L25 is not controlled by an autogenous (pre-)mRNA-targeted mechanism similar to that operating in bacteria, but rather by extremely rapid degradation of excess protein produced.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00405095
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Induced recombination and reversion of theCDC8 gene in relation to the cell cycle of yeast (1988)
    Springer
    Current genetics 13 (1988), S. 455-460 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast ; Gene conversion and mutation ; CDC8 locus ; Cell cycle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The induction of mitotic recombination in theCDC8 locus was studied in a diploid strain heteroallelic forcdc8 mutations (cdc8-1/cdc8-3); mitotic reversion was studied in strainscdc8-1/cdc8-1 andcdc8-3/cdc8-3. Conversion and reversion did not occur in those cells blocked at the S stage of the cell cycle by exposure to a nonpermissive temperature. In stationary phase cells irradiated just prior to exposure to temperature stress, the induction of recombinants was rather low and the induction of revertants was minimal. Conversely, a significant induction ofcdc + occurred in logarithmic phase cells subjected to the same treatment. Irradiation of synchronously dividing cultures revealed that intragenic recombination occurs at all three stages of the cell cycle- G1, S and G2. It was also found that UV-induced gene reversion can occur during the S and G2 stages, but not during the G1 stage of the cell cycle.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02427750
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  • 9
    Digitale Medien
    Digitale Medien
    Genetic mapping of eleven spo genes essential for ascospore formation in the fission yeast Schizosaccharomyces pombe (1986)
    Springer
    Current genetics 10 (1986), S. 443-447 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Mapping ; Sporulation ; Yeast ; Schizosaccharomyces pombe
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Sporulation-deficient mutants of the fission yeast Schizosaccharomyces pombe were isolated from a homothallic strain mutagenized with ethyl methanesulfonate. Complementation tests defined two new genetic loci (spo19 and spo20) essential for ascospore formation, in addition to the 18 known spo loci (Bresch et al. 1968). A novel mapping procedure using random spore analysis prior to tetrad analysis allowed us to map 11 spo genes. Four genes (spo3, spo15, spo19 and spo20) were mapped on chromosome I, 6 genes (spo2, spo4, spoS, spo6, spo14 and spo18) on chromosome II and 1 gene (spo13) on chromosome III. Although there was no noticeable clustering of spo genes on the chromosomes, three pairs of linked genes (spo15-spo20, spo3-spo19 and spo2-spo18) were found.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00419871
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  • 10
    Digitale Medien
    Digitale Medien
    Cloning and characterisation of the ribosomal RNA genes of the dimorphic yeast, Yarrowia lipolytica (1986)
    Springer
    Current genetics 10 (1986), S. 449-452 
    Details
    ISSN: 1432-0983
    Schlagwort(e): rRNA genes ; Yeast ; Yarrowia lipolytica
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The ribosomal RNA genes of Yarrowia lipolytica have been identified, both in restriction digests of total genomic DNA and in a pBR322 gene bank, by hybridisation with cloned Saccharomyces cerevisiae rDNA. The Y. lipolytica rDNA repeat unit is 8.9 kb in size and contains the genes for the 25S and 18S, but not the 5S, rRNA species. The number of copies of these repeat units is approx. 50 per haploid genome. Several clones were found which did not conform to the standard restriction map due to differences outside the coding region. It appears that there is either heterogeneity of the spacer sequence within a strain or that the Y. lipolytica rDNA genes may be present as a number of separate clusters within this yeast's genome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00419872
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