Summary
The ribosomal RNA genes of Yarrowia lipolytica have been identified, both in restriction digests of total genomic DNA and in a pBR322 gene bank, by hybridisation with cloned Saccharomyces cerevisiae rDNA. The Y. lipolytica rDNA repeat unit is 8.9 kb in size and contains the genes for the 25S and 18S, but not the 5S, rRNA species. The number of copies of these repeat units is approx. 50 per haploid genome. Several clones were found which did not conform to the standard restriction map due to differences outside the coding region. It appears that there is either heterogeneity of the spacer sequence within a strain or that the Y. lipolytica rDNA genes may be present as a number of separate clusters within this yeast's genome.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnitz J, Cramer JH, Rownd RH, Cooley L, Soll D (1982) FEBS Lett 143:129–132
Bayev AA, Georgiev OI, Hadjiolov AA, Kermechiev MB, Nicholaev N, Skryabin KG, Zakharyev VM (1981) Nucleic Acids Res 8:4919–4962
Bell GI, DeGennaro LJ, Gelfand DH, Bishop RJ, Valenzuela P, Rutter WJ (1977) J Biol Chem 252:8118–8125
Bollen AP (1982) Organization of fungal ribosomal RNA genes. In: Busch H (ed) The cell nucleus, vol 10. Academic Press, New York London, pp 67–175
Borsuk PA, Nagiec MM, Stepien PP, Bartnik E (1982) Gene 17:147–152
Cihlar RL, Sypherd PS (1980) Nucleic Acids Res 8:793–804
Denhardt DT (1966) Biochem Biophys Res Commun 23:641–646
Free SJ, Rice PW, Metzenberger RL (1979) J Bacteriol 137:1219–1226
Gaillardin CM, Charoey V, Heslot H (1973) Arch Microbiol 92:69–83
Grunstein M, Hogness DS (1975) Proc Natl Acad Sci USA 72:3961–3965
Lockington RA, Taylor GD, Winther M, Scazzocchio C, Davies RW (1982) Gene 20:135–137
Maizels N (1976) Cell 9:431–438
Orgryzdiak DM, Mortimer RK (1977) Genetics 87:621–632
Petes TD, Hereford LM, Skryabin KG (1978) J Bacteriol 134:295–305
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251
Rozek CE, Timberlake WE (1979) Nucleic Acids Res 8:1567–1578
Southern EM (1975) J Mol Biol 98:503–517
Specht CA, DiRusso CC, Novotny CP, Ullrich RC (1982) Anal Biochem 119:158–163
Tabata S (1980) Eur J Biochem 110:107–114
Van Heerikhuizen H, Ykema A, Klootwijk J, Gaillardin C, Ballas C, Fournier P (1986) Gene (in press)
Verbeet PPL, Klootwijk J, Van Heerikhuizen H, Fontijn R, Vreugdenhil E, Planta R (1983) Gene 23:53–63
Williamson DH, Fennell DJ (1975) Methods Cell Biol 12:335–351
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Clare, J.J., Davidow, L.S., Gardner, D.C.J. et al. Cloning and characterisation of the ribosomal RNA genes of the dimorphic yeast, Yarrowia lipolytica . Curr Genet 10, 449–452 (1986). https://doi.org/10.1007/BF00419872
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00419872