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  • Electron microscopy  (27)
  • reservoir  (23)
  • Springer  (50)
  • American Chemical Society
  • Annual Reviews
  • Blackwell Publishing Ltd
  • 2005-2009
  • 1990-1994
  • 1980-1984  (50)
  • 1982  (50)
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  • Springer  (50)
  • American Chemical Society
  • Annual Reviews
  • Blackwell Publishing Ltd
Years
  • 2005-2009
  • 1990-1994
  • 1980-1984  (50)
Year
  • 1
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    Colloid & polymer science 260 (1982), S. 564-569 
    ISSN: 1435-1536
    Keywords: lin. Polyethylene ; Single crystals ; Heat of Fusion ; DSC ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Recently published results for solution crystallized PE single crystals have shown, that the experimental heat of fusionΔH * is higher, if the solvent is exchanged to silicon oil (oil suspension samples) as compared with dried mats. This has been interpreted by the collapse of the original hollow pyramids during drying, inducing lateral defects within the lamellae. The present investigation does not confirm this unexpected result.ΔH * of dried mats (T c 66 to 91 °C) and of the corresponding oil suspension samples agree within the rather small limits of experimental error. The crystallinities as derived fromΔH *, density or WAXS are in excellent agreement. SEM micrographs of cold fractured dried mats show their spongy macromorphology, but TEM micrographs of stained ultra-thin sections reveal the lamellar morphology of the walls, consisting of curved lamellae and stacked hollow pyramides. If a dried mat is sintered at room temperature, a dense transparent film is obtained with a rather regular stacked morphology of large flat lamellae.ΔH * of these films agrees with that of the original mat.
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  • 2
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    Development genes and evolution 191 (1982), S. 205-207 
    ISSN: 1432-041X
    Keywords: Chitin inhibition ; Nikkomycin ; Cuticle ; Electron microscopy ; Epilachna varivestis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleoside antibiotic nikkomycin has proved to be an effective inhibitor of chitin synthesis in the Mexican bean beetleEpilachna varivestis. Ultrastructural investigations show defects in the procuticular area after nikkomycin application which suggest the complete absence of chitin. A cuticle like this is inflexible and too brittle to satisfy its normal function as an exoskeleton. The individuals are not able to free themselves from the exuvia and finally die. Therefore nikkomycin seems to be a potential insecticide with high specifity.
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  • 3
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    Archives of microbiology 132 (1982), S. 10-13 
    ISSN: 1432-072X
    Keywords: Calcofluor White ; Cell wall structure ; Chlorella ; Electron microscopy ; Protoplast ; Ruthenium Red
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Among 12 strains ofChlorella ellipsoidea, C. vulgaris, andC. saccharophila tested, 4 strains (1,C. ellpsoidea; 2,C. vulgaris; 1,C. saccharophila) formed osmotically labile protoplasts after treatment with mixtures of polysaccharide degrading enzymes. The relationship between enzymatical digestibility and structure or composition ofChlorella cell walls were studied by electron microscopy and staining techniques with some specific dyes. The cell wall structures of the 12Chlorella strains were grouped into three types: (1) with a trilaminar outer layer, (2) with a thin outer monolayer, and (3) without an outer layer. Protoplasts were formed only from the strains with a cell wall of Type 2. In the strains with a cell wall of Type 1, the outer layer protected the inner major microfibrillar layer against enzymatic digestion. The cell wall of Type 3 was totally resistant to the enzymes; the chemical composition of the cell wall would be somewhat different from that of other types.
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  • 4
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    Archives of microbiology 133 (1982), S. 97-99 
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Thylakoid centers ; Photosynthetic membranes/thylakoids ; Membranes ; Membrane biogenesis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An ultrastructural study of four cyanobacteria (Anabaena cylindrica, Dermocarpa violaceae, Gleocapsa alpicola, Pleurocapsa minor) indicates the presence of previously undescribed thylakoid centers from which photosynthetic membranes (thylakoids) radiate. These peripherally located thylakoid centers are cylinders 30 nm wide by 320 nm long, consisting of globular subunits oriented in nonparallel stacked arrays. Thylakoids are attached to the outer surface of the cylinder along its longitudinal axis. Thylakoid centers appear to be functionally significant due to their structure, location and thylakoid association.
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  • 5
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    Archives of microbiology 131 (1982), S. 116-123 
    ISSN: 1432-072X
    Keywords: Cell wall ; Wall degradation ; Lysozyme ; Autolysines ; Electron microscopy ; Staphylococcus aureus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In contrast to former findings lysozyme was able to attack the cell walls ofStaphylococcus aureus under acid conditions. However, experiments with14C-labelled cell walls and ribonuclease indicated that, under these conditions, lysozyme acted less as an muralytic enzyme but more as an activator of pre-existing autolytic wall enzymes. Electron microscopic studies showed that under these acid conditions the cell walls were degraded by a new mechanism (i.e. “attack from the inside”). This attack on the cell wall started asymmetrically within the region of the cross wall and induced the formation of periodically arranged lytic sites between the cytoplasmic membrane and the cell wall proper. Subsequently, a gap between the cell wall and the cytoplasmic membrane resulted and large cell wall segments became detached and suspended in the medium. The sequence of lytic events corresponded to processes known to take place during wall regeneration and wall formation. In the final stage of lysozyme action at pH 5 no cell debris but “stabilized protoplasts” were to be seen without detectable alterations of the primary shape of the cells. At the same time long extended ribbon-like structures appeared outside the bacteria. The origin as well as the chemical nature of this material is discussed. Furthermore, immunological implications are considered.
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  • 6
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    Protoplasma 111 (1982), S. 206-214 
    ISSN: 1615-6102
    Keywords: Bark beetle ; Sensilla ; Chemoreceptors ; Mechanoreceptors ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The antennal sensilla inI. typographus are almost exclusively confined to the flattened terminal flagellar segment. The sensillar types have distinct distribution patterns in the three areas where they are found. Judging from the ultrastructural characteristics the following functions can be assigned to the sensillar types: chemoreception, single-walled and double-walled sensilla; chemoreception/mechanoreception, terminal-pore sensillum. Moreover there are two types of mechanoreceptors, one of which is connected to a bristle, whereas the other terminates within the cuticle of the flagellar segment.
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  • 7
    ISSN: 1573-5117
    Keywords: zooplancton ; phénologie ; mise en eau ; reservoir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper presents the results of an investigation which lasted three years, on the effects of impounding a river on its zooplankton community. Forty zooplankton samples were collected from the Desaulniers River and from a string bog (53°35′N, 77°35′W) in 1976 before impoundment. Sampling was also conducted in 1977 (331 samples) and 1978 (153 samples) during and after impoundment. The population dynamics of zooplankton show different patterns in 1977 and 1978. The main impacts of impoundment on the river and the bog communities are the increase of the population density, particulary of Rotifera and Copepoda, the increase of the number of species of Rotifera and the increase of the diversity indices based on numbers and biomass. These changes are observed at each sampling station. The total biomass increases in lotic stations but decreases in the string bog because the density and the biomass of Cladocera increases in the river but drops in the bog. Results are compared to those obtained in natural lakes and bog of the same area and to those published in other studies.
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  • 8
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    Hydrobiologia 88 (1982), S. 55-55 
    ISSN: 1573-5117
    Keywords: reservoir ; education
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 9
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    Hydrobiologia 88 (1982), S. 117-133 
    ISSN: 1573-5117
    Keywords: phytoplankton ; reservoir ; succession ; abundance ; control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of a seasonal succession of phytoplankton species in Rutland Water is described. The factors controlling their succession and abundance are discussed in relation to changes in physical and chemical variables. At present, input of water from the catchment and by pumping from the Rivers Welland and Nene, has the greatest influence on phytoplankton abundance.
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  • 10
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    Hydrobiologia 88 (1982), S. 159-169 
    ISSN: 1573-5117
    Keywords: Chironomidae ; bathymetric ; distribution ; Rutland Water ; reservoir ; SPSS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bathymetric distribution of the eight most abundant taxa of chironomid larvae is described from benthic grab samples collected in Rutland Water from May 1977 to April 1979. Cricotopus, Psectrocladius and Endochironomus larvae were generally restricted to water less than 9 m deep. Procladius, Chironomus, Microtendipes, Polypedilum and Tanytarsini larvae were all recorded at depths down to 25 m. However, the highest population densities of the latter five taxa were recorded in shallow water. The results are discussed in relation to the diet of trout and certain management practices at the reservoir. Also described is the application of SPSS (Statistical Package for the Social Sciences) to the analysis of these data.
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  • 11
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    Hydrobiologia 88 (1982), S. 211-224 
    ISSN: 1573-5117
    Keywords: nature reserve ; reservoir ; planning ; design ; management ; wildfowl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper illustrates how practical conservation management has helped to create a nature reserve of international importance for wildfowl. The development of existing and introduced habitats has produced a wide diversity of fauna and flora without causing an unnatural ecological balance and close liaison with other reservoir users has establoshed zoning areas free from disturbance resulting in both man and birds benefiting from such agreements.
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  • 12
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    Hydrobiologia 89 (1982), S. 177-188 
    ISSN: 1573-5117
    Keywords: Chironomidae ; littoral ; reservoir ; salinity ; chlorides ; particulate organic matter ; temperature ; wind
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lake Texoma in southcentral Oklahoma was formed by the impoundment of the Washita and Red Rivers. The Red River is more highly saline than the Washita and creates a complex salinity gradient across the reservoir. Populations of chironomids were monitored with multiple-plate samplers in areas of high (34–113 mg l−1 Cl−), intermediate (35–60 mg l−1 Cl−) and low (4–27 mg l−1 Cl−) salinity during the spring and summer of 1978. Food availability, temperature, salinity and wind direction influenced the distribution of the 14 genera and at least 22 species of chironomids which colonized the multiple-plate samplers. Filter-feeders attained their highest densities in the river-arm stations where levels of particulate organic matter (POM) were high. Algal grazers attained their highest densities in the clear intermediate area where the plates of the samplers were covered with algal mats. Most of the genera believed to be feeding primarily on POM decreased in density as the temperature and density of Glyptotendipes sp. rose. Certain species were restricted to either the Red River arm or the Washita River arm and this is probably a reflection of different salinity tolerances.
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  • 13
    ISSN: 1573-5117
    Keywords: benthos ; depth distribution ; Diptera ; dragonfly ; Ephemeroptera ; Odonata ; reservoir ; South Carolina ; thermal effluent ; Trichoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution and abundance of macroinvertebrates along a water depth gradient were studied in thermal and ambient temperature areas of a reservoir in the southeastern United States. Benthic samples were taken at 10 depths (0.16–5 m) during January–April, 1978. Factors affecting the depth distribution of Ceratopogonidae, Chaoboridae, Chironomidae, Ephemeroptera, Odonata, and Trichoptera are discussed. The relationship between abundance of organisms and water depth was more complex than an inverse relationship generally reported in the literature.
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  • 14
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    Hydrobiologia 88 (1982), S. 43-46 
    ISSN: 1573-5117
    Keywords: Rutland Water ; landscape ; amenity planning ; tree planting ; reservoir ; draw-down
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The landscape problems encountered at Rutland Water were: 1) the reconciliation of the reservoir and its works with the rural surroundings; 2) siting and treatment of all ancillary works; 3) mitigation of exposure of shore by draw-down; 4) the provision of opportunities for recreation without damage to the environment; and 5) conservation of nature. The means adopted were: 1) forward planning; 2) consultation and co-operation with all interests concerned; 3) extensive planting of native species and of ground shaping; and 4) arrangements for appropriate maintenance.
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  • 15
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    Hydrobiologia 88 (1982), S. 93-101 
    ISSN: 1573-5117
    Keywords: reservoir ; raw water ; management ; chemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The options available for the management of Rutland Water are identified and their use discussed. The changes in the chemistry of the stored water with regard to sulphate, silica (molybdate-reactive), dissolved reactive phosphate and total oxidised nitrogen are shown.
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  • 16
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    Hydrobiologia 91-92 (1982), S. 623-639 
    ISSN: 1573-5117
    Keywords: reservoir ; sedimentation ; River Nile ; High Aswan Dam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The River Nile receives most of its sediment load from the Atbara and Blue Nile rivers, which carry eroded sediments north from the Ethiopian mountains during the seasonal flood period between August and October. Prior to the construction and operation of the High Aswan Dam, in 1964, 9–10 × 106 metric tons of suspended sediment were deposited annually in the flood plain of the Nile, while about 93% of the total average annual suspended load of 124 × 106 metric tons was carried out into the Mediterranean Sea. Since the full operation of the High Aswan Dam in 1968, the flood discharge of the Nile, below the dam, has been greatly modified and more than 98% of the total suspended load has been retained within the reservoir. Based on long-term records; estimated relationships between discharge and suspended load, and field measurements, the life span of the dead storage capacity has been estimated at a minimum of about 360 years. Although this preliminary calculation is less than the estimated design capacity of 450 years, it is expected that progressively more suspended solids will be released in the outflow of the reservoir and that together with the use of flood diversion schemes the High Aswan Dam is likely to approach its design life span.
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  • 17
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    Hydrobiologia 91-92 (1982), S. 623-639 
    ISSN: 1573-5117
    Keywords: reservoir ; sedimentation ; River Nile ; High Aswan Dam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The River Nile receives most of its sediment load from the Atbara and Blue Nile rivers, which carry eroded sediments north from the Ethiopian mountains during the seasonal flood period between August and October. Prior to the construction and operation of the High Aswan Dam, in 1964, 9–10 × 106 metric tons of suspended sediment were deposited annually in the flood plain of the Nile, while about 93% of the total average annual suspended load of 124 × 106 metric tons was carried out into the Mediterranean Sea. Since the full operation of the High Aswan Dam in 1968, the flood discharge of the Nile, below the dam, has been greatly modified and more than 98% of the total suspended load has been retained within the reservoir. Based on long-term records; estimated relationships between discharge and suspended load, and field measurements, the life span of the dead storage capacity has been estimated at a minimum of about 360 years. Although this preliminary calculation is less than the estimated design capacity of 450 years, it is expected that progressively more suspended solids will be released in the outflow of the reservoir and that together with the use of flood diversion schemes the High Aswan Dam is likely to approach its design life span.
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  • 18
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    Hydrobiologia 96 (1982), S. 177-184 
    ISSN: 1573-5117
    Keywords: sediment ; reservoir ; stream ; macroinvertebrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effects of sediment release from Guernsey Reservoir on macroinvertebrates of the North Platte River, Wyoming, were investigated during summer 1981. Suspended solids concentrations during sediment release increased from 〈20 mg l−1 to 〉300 mg l−1. Because fine particulates remained in suspension, mean particle size of substrates was unaltered. Densities of chironomids decreased 90% + during sediment release but recovered to initial levels in 3 weeks after the release ended. Densities of mayflies and oligochaetes increased. Changes in benthic populations were highly correlated with increases in suspended solids.
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  • 19
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    Hydrobiologia 88 (1982), S. 52-52 
    ISSN: 1573-5117
    Keywords: reservoir ; planning policies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The poster summarised Leicestershire County and Rutland District Councils Planning policies operative in the area surrounding Rutland Water. The policies are contained in the Structure Plan for Rutland, approved in December 1979, Rutland District Council's Settlement Policy and the Empingham District Plan preliminary discussion document. The policies of more specific application to the area surrounding Rutland Water are those of the Empingham District Plan which relate to land use, recreation and traffic management in the Parishes of Burley, Edith Weston, Egleton, Empingham, Exton, Gunthorpe, Hambleton, Horn, Lyndon, Manton, Normanton and Whitwell. The theme reflected in all policies covering this area is one of conservation. It is expressed in one of the Structure Plan's basic policies as follows: ‘Rutland's Planning Role should be one of positive conservation particularly of natural resources with an emphasis on minimising the loss of, and where practicable improving the natural assets of farmland, woodland, minerals and water.’
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  • 20
    ISSN: 1573-5117
    Keywords: reservoir ; Langmuir circulation ; algae ; sedimentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The quantitative extent to which the large-scale organised water motion in the surface waters of lakes and reservoirs, known as Langmuir circulation, affects the distribution and settling of algae and other suspended particles is not known and has thus been ignored in conventionally used water quality models. Since the distribution and settling of these particles is important in determining water quality, this study set out to investigate these effects using a mathematical model based on the two-dimensional advection-diffusion mass transport equation describing the temporal and spatial distribution of suspended particles in a typical Langmuir cell. The Langmuir circulation flow field and turbulent diffusion coefficients are empirically modelled by relating them to environmental parameters (Buranathanitt et al., in press). The results show that Langmuir circulation does affect particle distribution and settling. For particles with small sinking speeds, such as the lighter algae, the circulation causes intense mixing, resulting in essentially uniform distribution of particles over the cell (as assumed in the ‘well-mixed compartment model’). For particles with high sinking velocities, however, aggregation can occur, giving rise to significant reduction in sinking loss. For diatoms, effective sinking speeds are as much as 6% less than when cells are not operating while for coarse silt particles in a cell of large width to depth ratio a reduction of more than 60% is possible. Two preliminary laboratory experiments have also been performed in order to obtain further information on both the physical characteristics of Langmuir cells and their effects on suspended particles. The results of a wind-wave tank experiment suggest that the Langmuir circulation scale of motion is related to the height of the surface waves, thus providing an empirical means of determining the size of Langmuir cells from environmental variables. A particle settling tank experiment confirmed, in a qualitative way, the results obtained in the analytical study, with both the uniformly mixed and aggregation phenomena being reproduced (Buranathanitti et al., submitted). Full details of the work described in this abstract will be published elsewhere (Buranathanitt et al., in press; Buranathanitt et al., submitted).)
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  • 21
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    Hydrobiologia 88 (1982), S. 179-189 
    ISSN: 1573-5117
    Keywords: reservoir ; trout ; angling ; impoundment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rutland Water was stocked with trout in 1975 after removing the existing fish populations. Growth and survival of rainbow and brown trout are compared with the reservoir's filling regime, and re-colonisation by other fish species is described. The use of angler success as an indicator of fish stock density and its management is discussed.
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  • 22
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    Hydrobiologia 88 (1982), S. 147-156 
    ISSN: 1573-5117
    Keywords: reservoir ; benthos ; invertebrates ; colonisation ; abundance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The colonisation of Rutland Water by benthic invertebrates has been monitored from 1976 when the reservoir was first filled. A modified Ekman grab was used to sample populations quantitatively while false substrates and dredge samples were used to obtain supplementary data. Hand net and hand-sorting were used to study the littoral zone. The dominant taxa in the reservoir are Asellus aquaticus, A. meridianus, Gammarus pulex, Crangonyx pseudogracilis, Helobdella stagnalis, Erpobdella octoculata, Polycelis tenuis, larval Chironomidae and Oligochaeta. Other species which appeared abundantly, such as Limnaea pereger and Planorbis albus, have now disappeared and it is suggested that the generally low levels of gastropods might account for the poor performance of species such as Dugesia lugubris and Glossiphonia complanata. Other aspects of abundance are discussed, as are the effects of maturation of the system.
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  • 23
    ISSN: 1573-5117
    Keywords: trout ; Salmo trutta ; Salmo gairdneri ; diet ; Rutland Water ; reservoir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The diet of brown trout (Salmo trutta L.) and rainbow trout (S. gairdneri Richardson) in Rutland Water were compared during the first two fishing seasons (April–October 1977 and 1978). Fortnightly samples of approximately forty stomachs were obtained from boat and bank, rod-and-line caught trout giving a total of 1046 stomachs over the two seasons. During 1977 seasonal changes in the diet were divided into two phases; the first being a period of abundant drowned terrestrial food until June. This was followed by a period of more stable water level from July onwards when chironomid larvae and pupae were consistently the most important food items and the diversity of food also increased. In 1978 the proportion of chironomid pupae and larvae declined and they were replaced in the diet by Gammarus and Asellus.
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  • 24
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    Hydrobiologia 88 (1982), S. 7-17 
    ISSN: 1573-5117
    Keywords: Rutland Water ; reservoir ; water supply ; demand ; design ; recreation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rutland Water was planned in the 1960s to meet the water needs of the expanding East Midland region of England. Chosen from 64 possible sites, it was approved in 1970 and construction started in 1971. The dam was closed and the reservoir started to fill from its natural catchment in 1975; pumping started from the rivers Welland and Nene in 1976 and the reservoir was full by March 1979. Details of the stages in its development are given together with an indication of the ways in which the recreational uses of the reservoir have been integrated with its water supply function.
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  • 25
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    Hydrobiologia 88 (1982), S. 191-198 
    ISSN: 1573-5117
    Keywords: trout ; littoral fauna ; impoundment ; flooding ; reservoir ; Rutland Water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fingerling Salmo trutta L. and S. gairdneri R. were stocked in Rutland Water in early 1975. Initial rapid growth was associated with feeding on littoral and planktonic Crustacea during this year. Rapid water level changes occurred over the winter months, interspersed with longer periods of stability during the summer. These changes caused changes in trout diet. Terrestrial food (e.g. earthworms), released from flooded land was important during winter months; aquatic items which increased in abundance during periods of stable water levels (e.g. Gammarus pulex, Limnaea pereger, Chironomidae larvae and pupae) were important during spring, summer and autumn. It is suggested that the rapid growth rates recorded in the first two years of the reservoir are the result of the ready availability of different food sources.
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  • 26
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    Hydrobiologia 91-92 (1982), S. 623-639 
    ISSN: 1573-5117
    Keywords: reservoir ; sedimentation ; River Nile ; High Aswan Dam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The River Nile receives most of its sediment load from the Atbara and Blue Nile rivers, which carry eroded sediments north from the Ethiopian mountains during the seasonal flood period between August and October. Prior to the construction and operation of the High Aswan Dam, in 1964, 9–10 × 106 metric tons of suspended sediment were deposited annually in the flood plain of the Nile, while about 93% of the total average annual suspended load of 124 × 106 metric tons was carried out into the Mediterranean Sea. Since the full operation of the High Aswan Dam in 1968, the flood discharge of the Nile, below the dam, has been greatly modified and more than 98% of the total suspended load has been retained within the reservoir. Based on long-term records; estimated relationships between discharge and suspended load, and field measurements, the life span of the dead storage capacity has been estimated at a minimum of about 360 years. Although this preliminary calculation is less than the estimated design capacity of 450 years, it is expected that progressively more suspended solids will be released in the outflow of the reservoir and that together with the use of flood diversion schemes the High Aswan Dam is likely to approach its design life span.
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  • 27
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    Hydrobiologia 88 (1982), S. 51-51 
    ISSN: 1573-5117
    Keywords: tree-planting ; reservoir ; drawdown
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aesthetic problems posed by reservoir margins have been recognised for many years. They stem from a complex pattern of action and interaction of several environmental variables — water manipulation patterns, wave action, substrate characteristics, grazing and other factors. These factors usually result in reservoir margins being not only visually unattractive and physically unstable, but also of low ecological diversity. The Natural Environment Research Council and Water Research Centre have, since 1968, together funded a research programme to investigate the use of plant species to combat these problems. In isolated instances in the UK, woody species in particular have succeeded in colonising margins naturally, to very good visual effect (e.g. at Lake Vyrnwy). ‘Biotechnical’ treatments — using artificially planted shrubs and trees — have been successfully used in large Central European reservoirs to combat soil erosion by waves. Following trials of various woody species on the margins of established reservoirs, 10 species/cultivars were ‘pre-planted’ above and below the future top water level (TWL) of Rutland Water in 1973, 5 years before reservoir filling was completed. Pre-planting theoretically allows better survival by trees of subsequent partial inundation. The precise siting of pre-planted trees in the vertical dimension should normally be done using modelled predictions of reservoir summer median water level (a variable important to tree survival). The complex factors affecting the operation of Rutland Water made precise prediction impossible; the experimental plot eventually extended from 500 mm above TWL, to 2 000 mm below TWL, in 500 mm steps. 252 trees were involved in the plot and their growth has been monitored for the past 7 years. The species being predominantly hydrophytic, drought was a problem in the years prior to reservoir filling. Mulching with grass-cuttings proved to be helpful in this respect. Subsequently, prolonged periods of high water level have been deleterious to tree survival in the lower areas of the plot. Once abstraction/recharging patterns become more cyclic, it is anticipated that tree growth will stabilise.
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    Hydrobiologia 88 (1982), S. 53-54 
    ISSN: 1573-5117
    Keywords: reservoir ; visitors ; traffic ; amenity
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    Hydrobiologia 88 (1982), S. 103-116 
    ISSN: 1573-5117
    Keywords: reservoir ; treatment ; pulsator ; quality ; haloforms
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    Topics: Biology
    Notes: Abstract The paper describes the treatment facilities and processes at the Wing Works where Rutland water is treated to potable standards. It summarises the experience gained in the operation of the works since it opened in 1977 and includes a brief account of an investigation into the production of haloforms.
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    Cell & tissue research 226 (1982), S. 589-608 
    ISSN: 1432-0878
    Keywords: Kidney (frog) ; Glomerulus ; Nephron ; Tight junctions ; Freeze-fracturing ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary By the use of thin sections and freeze-fracture replicas the glomerular and tubular structures of the kidney of the frog (Rana esculenta) were studied with special reference to intercellular junctions. In the glomerulus the filtration barrier is of very variable thickness, and frequent tight and gap junctional contacts occur between podocyte processes. Although structurally less elaborate, the proximal tubule resembles its mammalian counterpart. In the initial part the tight junctions are relatively shallow but become very broad in the mid and distal portions of the proximal tubule. The proximal tubular cells are extensively linked by gap junctions. In some animals the shapes of the cells in the proximal and distal portions of the proximal tubule were markedly different. The distal tubule consists of two segments which differ mainly in the pattern of interdigitations and the structure of the zonulae occludentes. Similarities with the tight junctional morphology of the mammalian distal tubule are striking. In the first part of the distal tubule (diluting segment) a narrow band of parallel tight junctions is found closely resembling that found in the mammalian straight distal tubule; in the more distal part of the distal tubule, however, a broad band of anastomosing tight junctional strands exists, like the zonula occludens of the mammalian convoluted distal tubule. The connecting tubule displays cellular dimorphism: its wall contains a mixture of light and dark (flask) cells. The luminal and basolateral membranes of the flask cells are covered with numerous rod-shaped particles. The tight junctions of the connecting tubule are broad and increase in depth and number of strands along its length; they are typical of a very tight epithelium. In spite of several dissimilarities with phylogenetically younger kidneys our findings suggest that many structural principles of the mammalian kidney are also represented in the kidneys of amphibians. The structural-functional relationships are discussed.
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  • 31
    ISSN: 1432-0878
    Keywords: Stomach (Teleost) ; Endocrine cells ; Electron microscopy
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    Notes: Summary In the gastric mucosa of two teleost species, the perch (Perca fluviatilis) and the catfish (Ameiurus nebulosus) three endocrine cell types were found, located predominantly between the mucoid cells of the gastric mucosa. A fourth cell type is present in the gastric glands of catfish. Each cell type was defined by its characteristic secretory granules. Type-I cells were predominant in both fish. These cells contained round or oval granules with a pleomorphic core. The average diameter of granules was 400 nm for the perch and 270 nm for the catfish. Type-II cells of both species displayed small, highly osmiophilic granules about 100 nm in diameter. The secretory granules of type-III cells (260 nm in the perch and 190 nm in the catfish) were round or slightly oval in shape and were filled with a finely particulate electron-dense material. Type-IV cells of the catfish were found in the gastric glands only. Their cytoplasm was filled with homogeneous, moderately electron-dense granules averaging 340 nm in diameter. The physiological significance of these different morphological types of gastric endocrine cells requires further investigation.
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    Cell & tissue research 222 (1982), S. 409-415 
    ISSN: 1432-0878
    Keywords: Crustacean compound eye ; Eighth retinular cell ; Crystalline tract ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary The base of the crystalline tract, the distal part of the eighth retinular cell and its rhabdomer constitute a structural unit in the apical region of the retinula of Astacus fluviatilis and A. leptodactylus, shielded from the blood by a special covering cell.
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    Cell & tissue research 224 (1982), S. 637-645 
    ISSN: 1432-0878
    Keywords: Cell surface antigen ; Neurons ; Glia ; Electron microscopy ; Immunoperoxidase ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immuno-electron microscopy was performed on live, cultured, early postnatal cerebellar and retinal cells of the mouse to identify A2B5 antigenbearing elements. In cerebellar cultures, granule cells, some immature oligodendroglia, and astroblasts express A2B5 antigen on their cell surfaces. The typical features of astroblasts include large cisternae of the endoplasmic reticulum and a mixed population of intermediate-sized filaments and microtubules. Immature oligodendroglia cells express the antigen on their cell bodies and on procecesses filled with cytoplasm. Cytoplasm-free membranous whorls, however, are devoid of A2B5 antigen, but not of 0 or NS-1 antigens. In retinal cultures, A2B5 antigen is observed on differentiating neurons with the exception of photoreceptor cells as identified by ribbon synapses.
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    Cell & tissue research 224 (1982), S. 673-683 
    ISSN: 1432-0878
    Keywords: Pacinian corpuscles, rat ; Denervation ; Sensory terminals ; Nerve degeneration ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of Pacinian corpuscles located on the crural interosseous membrane was studied in adult rats 6 h to 10 months after transection of the right sciatic nerve. Axon terminals degenerated one day after transection and were engulfed and resorbed by cells of the inner core within one week. The axial space left after removal of the axonal debris was closed by the lamellae of the inner core. The main structural features of the inner core and capsule remained preserved after denervation throughout the period of study. The denervated inner cores, however, became atrophic 10 months after neurotomy, their mean diameter being reduced by 17.5% compared with that of contralateral control corpuscles. The number of capsular lamellae was unaltered, and perineurial pathways of the peripheral nerve stump remained preserved. Schwann cells proliferated and formed Büngner bands during the first month after denervation, but retracted their processes and became atrophic at later stages after neurotomy. Survival of Pacinian corpuscles after long-term denervation in adult rats is in contrast to their rapid degeneration within several days after nerve section in neonates.
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  • 35
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    Keywords: Electron microscopy ; Junctions ; Smooth muscle ; Echinodermata ; Holothuria, Aspidochirotida
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    Notes: Summary Electron microscopy reveals that sarcolemmata of adjacent muscle cells form pentalaminar junctions by fusion of apposed trilaminar double leaflet membranes. These junctions appear to be candidates for low resistance pathways between muscle fibers. The muscles depolarize slowly when bathed in solutions containing elevated concentrations of KCl, and the sucrose gap method can then be used to measure the potential difference between polarized and depolarized regions. Thus the junctions which we have observed may provide the structural basis for electrical transmission through the sucrose gap.
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  • 36
    ISSN: 1432-0878
    Keywords: Peritrophic membrane ; Tick ; Babesia ; Electron microscopy
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    Notes: Summary A peritrophic membrane (PM) has been demonstrated in the gut of feeding larvae, nymphs, and adults of the tick Ixodes dammini. This is the first report of a PM in ticks. This temporary structure divides the lumen of the gut into two compartments, an endoperitrophic space, the lumen proper, and an ectoperitrophic space located between the PM and the epithelial cells of the gut wall. The PM is a mechanical barrier and even such small particles as ribosomes derived from ingested reticulocytes are retained in the lumen proper; they are never found in the ectoperitrophic compartment. In Ixodes dammini fed on hamsters infected with Babesia microti some of the parasites are found in the ectoperitrophic space. This passage is accomplished by a highly specialized organelle, the arrowhead, which develops in some Babesia during their metamorphosis in the gut of the vector. The arrowhead, while passing through the PM, changes its fine structure and loses its internal organization as if releasing some of its contents. Its disintegration continues and it disappears shortly after the Babesia have entered the epithelial cells. Only Babesia equipped with the arrowhead structure are able to cross the PM. This is the first documented case of a parasite traversing a solidified PM.
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    Cell & tissue research 222 (1982), S. 25-40 
    ISSN: 1432-0878
    Keywords: Paddle cilia ; Discocilia ; Pleurobranchaea ; Chemoreceptors ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Scanning electron microscopy of various regions of the body of the marine gastropod Pleurobranchaea californica (McFarland) has revealed a characteristic cell type that bears cilia with dilated discoid-shaped tips. The tips of the cilia consist of an expansion of the ciliary membrane around a looped distal extension of the axoneme. These kinocilia have been observed in numerous other marine invertebrates and are generally referred to as paddle cilia (Tamarin et al. 1974) or discocilia (Heimler 1978). Although many functions have been proposed for paddle cilia, little empirical evidence supports any of the proposals. In Pleurobranchaea we have found that the distribution of this ciliated cell type corresponds exactly to areas of the body known from behavioral studies (Lee et al. 1974; Davis and Matera 1981) to mediate chemoreception. Transmission electron microscopy of the epithelium lining the rhinophores and tentacles of Pleurobranchaea revealed details of the ultrastructure of these ciliated cells and showed that they are primary receptors. These ciliated receptors lie in a yellow-brown pseudostratified columnar epithelium that superficially resembles the olfactory mucosa of vertebrates.
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    Cell & tissue research 222 (1982), S. 167-175 
    ISSN: 1432-0878
    Keywords: Pigment granules ; Chromatophores ; Granulogenesis ; Palaemonid shrimp ; Macrobrachium ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary The possible biogenesis of two pigment granule types present in the monochromatic, brown chromatosomes enveloping the ventral nerve chord of the freshwater palaemonid shrimps Macrobrachium acanthurus, M. heterochirus and M. olfersii is examined by transmission electron microscopy in thin section and freeze fracture replicas. Prominent, membrane limited granules are suggested to have their origin in a complex, juxtanuclear, smooth endoplasmic reticulum labyrinth, continuous with the nuclear envelope. Amembranous, lipocarotenoid granules possibly derive from the external surface of the smooth endoplasmic reticulum. Nuclear envelope and SER membranes contain numerous 11 nm diameter intramembranous particles while pigment granule membranes exhibit fewer particles. A dictyosomal origin for the lipocarotenoid granules is discounted. Granulogenesis is suggested to be a continuous process in crustacean chromatophores.
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    Cell & tissue research 222 (1982), S. 261-267 
    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Fiber types ; Electron microscopy ; Stereology ; Chicken
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    Topics: Biology , Medicine
    Notes: Summary Ultrastructural and stereological assessment of the mature avian anterior latissimus dorsi (ALD) muscle showed that it contains two kinds of extrafusal fibers. This fine structural dichotomy of fiber types in the ALD correlated well with their previously reported histochemical duality. Distinct differences occur in sarcomere banding, myofibrillar area, sarcotubular and mitochondrial density, and in morphology of motor-nerve terminals. Both myofiber types in this muscle were interpreted as representing varieties of “slow” or tonic muscle fibers. Both fibers contain myofibrils that, despite differences in cross-sectional area, were large, irregular, and ribbon-shaped, typical of the “Felderstruktur” appearance of true “slow” fibers. Whereas the majority of fibers (type-1) are devoid of well-defined M-bands, the minor fiber population (type-2) exhibit prominent M-bands in the center of each sarcomere. In addition, type-1 tonic fibers contain a significantly lower mitochondrial and sarcotubular volume than the tonic fibers of type-2. While both fiber types exhibit motor-nerve terminals that are small, smooth and punctate in appearance, those on the type2 fibers often had a number of shallow postjunctional folds. Whether or not these two classes of extrafusal fiber in this muscle represent two separate and distinct types of motor units remains to be determined functionally.
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    Cell & tissue research 223 (1982), S. 87-99 
    ISSN: 1432-0878
    Keywords: Smooth muscle cells ; In vitro-growth ; Whole blood serum ; Plasma-derived serum ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Normal diploid cells require serum to proliferate in culture. Platelet-derived growth factor has been identified as the main serum component responsible for this effect. Here, smooth muscle cells were isolated enzymatically from the aorta of 5-day-old rats and cultured in the presence of 10% whole blood serum (WBS) or plasma-derived serum (PDS), i.e. with or without platelet factor, and studied by transmission electron microscopy. The cells proliferated actively in WBS-medium but remained quiescent in PDS-medium. Fine structurally, cells from WBS-cultures demonstrated numerous mitochondria, an extensive rough endoplasmic reticulum (RER), a large Golgi complex, a few lysosomes, and microfilaments arranged in parallel bundles. After transfer to PDS-medium, the RER- and Golgi cisternae were markedly dilated and the number of membrane-associated ribosomes decreased. Segregation of fragments of cytoplasm within autophagosomes was frequently observed and the number of lysosomes increased. Lipid droplets were more abundant and often gathered in the Golgi area. Moreover, the cells had become more irregular in shape and showed many bleb-like processes at their surface. Microfilament bundles had also become more prominent and crossed each other in different directions. These observations show that the removal of platelet factor from the medium clearly modifies the fine structure of cultured smooth muscle cells. The findings are in good agreement with the concept that platelet factor not only supports the proliferation of cultured cells but also stimulates their secretory activity.
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    Cell & tissue research 223 (1982), S. 267-280 
    ISSN: 1432-0878
    Keywords: Retina (Astacus) ; Rhabdom ; Osmolarity ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Alterations of the retinula cells in the retina of the light-adapted crayfish in response to hyper- and hypoosmotic van Harreveld solutions was examined by transmission electron microscopy. Increased osmolarity of the bathing medium to twice that of the physiological value leads to shrinkage of the retinula-cell somata. Microvilli, on the other hand, do not undergo shrinkage. Some other characteristic irreversible changes do, however, take place, including detachment of microvilli from the soma, showing a two- or threefold increase in diameter, and a concomitant decrease in number, probably due to fusion of microvilli. Prolonged incubation or higher osmolarities (5 isosmol) cause alteration of the microvillar membranes to whorls. Structural changes are often restricted to microvillar stacks evaginating from certain individual retinular cells. The number of affected stacks increases depending on the duration of incubation or the osmotic pressure. Hypoosmotic solution (0.5 isosmol) also induces an increase of microvillar diameters and a concomitant reduction in number of microvilli per stack. Exposure to a 20% solution of glycerol causes destruction of the rhabdom structure and the formation of whorls from microvillar membranes. The present findings suggest that the structure of the microvilli is stabilized by an axial cytoskeleton.
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    Cell & tissue research 224 (1982), S. 291-301 
    ISSN: 1432-0878
    Keywords: Thymus (rat) irradiation ; Electron microscopy ; Interdigitating cells ; Macrophages ; Phagocytosis
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    Topics: Biology , Medicine
    Notes: Summary Phagocytic activity and population development of medullary interdigitating cells (IDC) and cortical macrophages have been studied in the rat thymus after irradiation-induced thymocyte necrosis. IDC clearly demonstrate phagocytic activity, particularly in the 16h stage after irradiation. At this stage the number of necrotic thymocytes is maximal and the total number of phagocytic cells is insufficient to phagocytize all necrotic material. IDC increase in number slightly and are predominantly phagocytizing in the corticomedullary region (CMR), where they appear to develop from macrophage-like cells. The results indicate that medullary IDC can be phagocytic but have a different developmental pathway than cortical macrophages. Cortical macrophages greatly increase in number and acquire the appearance of tingible body macrophages by phagocytizing many necrotic thymocytes. They seem to develop from monocytes that normally enter the thymus at the CMR. During the acute involution macrophages probably also enter the cortex via the connective tissue capsule. It is suggested that thymus medullary IDC probably belong to the mononuclear phagocytes, as do the cortical macrophages. In the specific medullary environment IDC gradually develop their characteristic ultrastructure for an apparently other than phagocytic function. The similarities between IDC and epidermal Langerhans cells are discussed.
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    Cell & tissue research 224 (1982), S. 349-359 
    ISSN: 1432-0878
    Keywords: Ovarian follicle ; Ovulation ; Ultrastructural change ; Fowl ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary No structural changes could be observed in the stigma until 2 h before ovulation. Within a few minutes of ovulation, the stratum granulosum and theca interna disappear completely in the middle part of the stigma. By 30 min before ovulation, the rough endoplasmic reticulum in the fibroblasts of the theca externa develops conspicuously. Then a few minutes before ovulation, the theca externa becomes much thinner with a conspicuous disintegration of the collagenous fibers into individual fibrils. These structural changes may contribute to the fragility of the stigma tissue and induce rupture of the follicle.
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    Cell & tissue research 224 (1982), S. 383-395 
    ISSN: 1432-0878
    Keywords: Mouse ovary ; Junctions ; Freeze-fracture ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Intercellular junctions in the young mouse ovary were examined by electron microscopy utilizing freeze-fracture and thin-sectioning techniques. Projections from the granulosa cells adjacent to the oocyte (GI) traverse the zona pellucida and form small gap junctions on the oocyte surface. On the P-face of these cells, the junctional aggregations are occasionally associated with linear strands of particles. In contrast, large gap junctional areas are frequently observed between the more peripherally located granulosa cells (GE) and are also present in the theca interna (TI) cell layer surrounding the follicles. Three types of tight junctional strands are discernible on the P-face of theca externa cells (TE): angularly zigzag strands consisting of intermittently distributed intramembranous particles on wide ridges, intermediate zigzag strands consisting of more continuously distributed particles, and wavy strands consisting of rather fused particles. Tight junctional strands are also present in the middle of grooves on the E-face of endothelial cells of blood vessels. In the germinal epithelial cell layer, tight junctional strands appear to be discrete and form a less anastomosing network.
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    Cell & tissue research 224 (1982), S. 369-381 
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Pars intermedia ; Electron microscopy ; Fetal sheep ; Lamb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using light and electron microscopy, the morphogenesis of the pars intermedia of the sheep pituitary gland was examined in developing lambs between 26 days of gestation and the newborn stage. Following the establishment of contact between the anterior and posterior lobe primordia seen at 26 days, the connection with the pharyngeal roof disappeared by 31 days. The lumen of Rathke's pouch, which was a prominent cavity at the earlier stages, became inconspicuous by 40 days but progressively increased in size during gestation and, in some newborn animals, contained colloid material. At 40 days, the pars intermedia consisted of a uniform population of undifferentiated cells. Cells with cytoplasmic granules were first identified at 50 days. The cytological appearance of granular cells at 70 days indicated increased synthetic activity and by 80 days they closely resembled adult glandular cells. At 100 days, membrane activity suggestive of exocytosis was first observed in granular cells; fenestrated capillaries were present, and early follicle formation between adjacent non-granular cells was seen. This apparent exocytotic release of granules was observed much more frequently between 100 days of gestation and the newborn stage than in adult pars intermedia cells. These findings indicate that glandular cells of the developing pars intermedia are actively engaged in synthesis, storage and secretion from an early stage.
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    Cell & tissue research 225 (1982), S. 355-364 
    ISSN: 1432-0878
    Keywords: Implantation ; Blastocyst ; Pontamine ; blue reaction ; Decidualization ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The early uterine response to transplanted, delayed and estrogenactivated blastocysts was studied ultrastructurally and compared with that induced by intrauterine instillations of deciduogenic agents (arachis oil, air). The uterine responses to delayed and activated blastocysts showed no ultrastructural or temporal differences. Already within 4 h after transfer to a sensitized uterus, the delayed blastocysts exhibited signs of activation, and both types of blastocysts had started to attach onto an undamaged epithelial lining. Signs of stromal cell differentiation into decidual cells were also seen as early as 4 h after transfer, while the Pontamine-blue reaction did not appear until after 8 h. The results therefore indicate that the transplanted blastocysts induced decidualization atraumatically and that the delayed blastocysts were either deciduogenic already before transfer or rapidly acquired deciduogenic properties after transfer. Artificial decidual induction with oil and air led to damage or death of a large number of cells in the uterine luminal epithelium. Within only 15 min after instillation pronounced signs of cell damage were seen, and later numerous cells were extruded from the epithelial lining. In the stroma ultrastructural signs of decidual cell differentiation and a Pontamine-blue reaction were observed as early as 4 h after induction. It is therefore suggested that oil and air induce decidualization via the epithelium by means of trauma.
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    Cell & tissue research 225 (1982), S. 443-448 
    ISSN: 1432-0878
    Keywords: Frog cerebellum ; Ependymal surface ; Cilia ; Supraependymal cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural studies of the ventricular surface of the frog cerebellum showed regional differences. In the midline region of the adult cerebellum was found a band of profusely ciliated squamous ependymal cells. In the rest of the cerebellum the ependymal cells were columnar and each had a single cilium. In the cerebellum of the premetamorphic tadpole, the squamous ependymal cells of the midline region also were monociliated. During metamorphosis they gradually became multiciliated. Additionally, supraependymal cells and synaptic elements were present on the ventricular surface of the cerebellum of adult frogs as well as in late metamorphic tadpoles. In contrast, supraependymal cells were rarely observed in premetamorphic tadpoles, and it was concluded that the supraependymal system develops during metamorphosis. It is postulated that the band of cilia may be associated with the circulation of cerebrospinal fluid, and supraependymal synaptic elements function in neuroendocrine regulation.
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    Cell & tissue research 225 (1982), S. 663-671 
    ISSN: 1432-0878
    Keywords: Chaetognatha ; Intestine ; Pleated septate junction ; Electron microscopy ; Freeze-fracture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The intramembrane structures of the pleated septate junction which occur in the junctional complex of the intestine of the chaetognath Sagitta setosa have been investigated. The pleated septate junction is made up of linear rows of irregularly shaped and sized particles, often fused into short rods, and pits which can be fused into furrows. The distribution of these structures on E and P faces depends upon the preparative methods used. Many of the morphological characteristics are the same as those of the “lower invertebrate pleated septate junction type” defined by Green (1981a). The physiological significance of this junction is obscure. On the basis of the presence of septate junctions (both of the paired septate junction and pleated septate junction types) which have mainly morphological characteristics of the “lower invertebrate pleated septate junction” we can add to the hypothesis that chaetognaths are not related to the molluscs and arthropods.
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  • 49
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    Keywords: Enteroendocrine cells ; Pancreatic endocrine cells ; Gastroenteropancreatic hormones ; Immunocytochemistry ; Electron microscopy
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    Notes: Summary Four immunoreactive endocrine cell types can be distinguished in the pancreatic islets of B. conchonius: insulin-producing B cells, somatostatin-producing A1 (= D) cells, glucagon-producing A2 cells and pancreatic poly-peptide-producing PP cells. The principal islet of this species contains only a few PP cells, while many PP cells are present in the smaller islets. Except for the B cell all pancreatic endocrine cell types are also present in the pancreatic duct. At least six enteroendocrine cell types are present in the gut of B. conchonius: 1. a cell type (I) with small secretory granules, present throughout the intestine, and possibly involved in the regulation of gut motility; 2. a C-terminal gastrin immunoreactive cell, probably producing a caerulein-like peptide; these cells are located at the upper parts of the folds, especially in the proximal part of the intestinal bulb; 3. a met-enkephalin-immunoreactive cell, present throughout the first segment; 4. a glucagon-immunoreactive cell, which is rare in the first segment; 5. a PP-immunoreactive cell, mainly present in the first half of the first segment; 6. an immunoreactive cell, which cannot at present be specified, located in the intestinal bulb. The latter four cell types are mostly located in the basal parts of the folds, although some PP-immunoreactive cells can also be found in the upper parts. Most if not all enteroendocrine cells are of the open type. The possible functions of all enteroendocrine cell types are discussed.
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    Cell & tissue research 226 (1982), S. 327-335 
    ISSN: 1432-0878
    Keywords: Xenopus ; Spleen ; B-lymphocyte ; Immunofluorescence ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An indirect immunofluorescence study of the frozen sections of the spleen of an anuran amphibian, Xenopus laevis, showed that lymphocytes bearing a small amount of immunoglobulin (Ig) were localized mostly in the white pulp of non-immunized toads. There were fewer fluorescent cells in the red pulp. In the toads hyperimmunized with human gamma globulin (HGG), cells with strong cytoplasmic fluorescence increased significantly in the outer part of the white pulp. Electron microscopy of spleens from these toads showed that plasma cells at different stages of maturation were abundant in the white pulp, whereas in the red pulp, a smaller number of maturer plasma cells were observed. These results indicate that, in contrast with its mammalian counterpart, the splenic white pulp of this anuran is the site where thymusin-dependent lymphocytes commence blast formation and transformation into plasma cells.
    Type of Medium: Electronic Resource
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