ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Yeast  (58)
  • temperature  (50)
  • Springer  (108)
  • Annual Reviews
  • Blackwell Publishing Ltd
  • 2005-2009
  • 1990-1994  (74)
  • 1980-1984  (34)
  • 1991  (74)
  • 1982  (34)
Collection
Publisher
Years
  • 2005-2009
  • 1990-1994  (74)
  • 1980-1984  (34)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 7 (1991), S. 191-195 
    ISSN: 1476-5535
    Keywords: Yeast ; Trehalose ; Osmotolerance ; Viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A total of 12 yeast strains from various genera were examined for their ability to produce ethanol in the presence of high concentrations of glucose. From these studies, the yeastsTorulaspora delbrueckii andZygosaccharomyces rouxii were observed to the most osmotolerant. These osmotolerant yeast strains were also observed to possess high concentrations of intracellular trehalose. Futhermore, these strains were found to be tolerant to long-term storage at −20°C and to storage at 4°C in beer containing 5% (v/v) ethanol. Cells containing high trehalose levels at the time of freezing or cold storage exhibited the highest cell viabilities. Trehalose concentration was observed to increase during growth on glucose, reaching a maximum after 24–48 h. Increasing the incubation temperature from 21 to 40°C also resulted in an increase in intracellular trehalose content. These results suggest that trehalose plays a role in enhancing yeast survival under environmentally stressful conditions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 7 (1991), S. 263-268 
    ISSN: 1476-5535
    Keywords: Yeast ; β-Glucanase ; β-Glucosidase catabolite repression ; Sporulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The activities of three glycosidases, β-glucosidase and β(1,3)- and β(1,6)-glucanases have been monitored during growth and blastospore formation inSaccharomycopsis fibuligera. The assays were carried out on the cell-free culture and in a cell-free extract and a wall autolysate preparation from the growing cells. In complex medium containing 1% glucose an increase in the level of all three enzymes was associated with the transition from mycelium to blastospores. When the level of glucose was increased to 5% blastospore formation was repressed and the level of β-glucanases only increased at the end of the fermentation. The β-glucosidase activity increased during the growth phase. In a defined medium in which slow growth in a wholly yeast-like form was observed, growth was not associated with a high level of β-glucanase activity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 58 (1991), S. 289-293 
    ISSN: 1570-7458
    Keywords: Spider mites ; Tetranchus urticae ; damage ; feeding ; temperature ; photoperiod
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Wir untersuchten den Einfluss von Temperatur und Licht auf die Saugtätigkeit von Tetranychus urticae auf Bohnenpflanzen und fanden eine nicht-lineare Beziehung zwischen Temperatur und Saugaktivität. Die Saugintensität stieg bei Temperaturen über 10 °C an bis zum Saugmaximum bei 35 °C und sank dann relativ rasch ab. Permanentes Licht- oder Dunkelregime übte keinen Einfluss auf die Saugleistung aus. Aufgrund der beobachteten Zusammenhänge zwischen Temperatur, Saugintensität und Intensität der Ausbildung der Schadsymptome entwickelten wir ein verbessertes Mass (‘mite-load’) für die Erfassung des Spinnmilbenstresses auf die Wirtspflanze. Die präsentierten Daten zeigen, dass mit der ‘mite-load’ Funktion die Saugschäden von T. urticae präziser erfasst werden können als mit den bisher gebräuchlichen Milbendichten pro Blatt oder Milbentagen.
    Notes: Abstract The influence of temperature and light regime on the feeding intensity of Tetranychus urticae (Koch) (Acari: Tetranychidae) was studied on bean plants. A nonlinear relationship was found between temperature and feeding activity of T. urticae. The feeding intensity increased from 10 °C to 35 °C. At 10 °C there was practically no feeding, whereas at 35 °C maximum feeding occurred. above 35 °C the activity of the mites decreased. No difference could be found in the feeding intensity of mites kept at permanent darkness or permanent light. Based on the observed relationship between temperature and feeding activity and intensity of damage symptoms, respectively, we propose the use of a mite-load function to define the mite stress imposed on the plant.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1570-7458
    Keywords: Beauveria bassiana ; Leptinotarsa decemlineata ; larval infection ; adult survivor ; fecundity ; egg hatchability ; inoculum dosage ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les effets secondaires de l'hyphomycète entomopathogène, Beauveria bassiana (Bals.) Vuill. sur la fécondité et la fertilité des insectes survivant à l'infection fongique ont été étudiés chez les adultes du doryphore Leptinotarsa decemlineata Say (Coleoptera:Chrysomelidae) issus de larves contaminées en début de 4ème stade. Les insectes maintenus à 22°C ont présenté une réduction de leur potentiel reproductif. Ainsi, suivant la dose d'inoculum fongique, le nombre total d'oeufs pondus par femelle et le nombre moyen d'oeufs par opplaque ont baissé de 20% à 56% et de 18% à 46%, respectivement. En revanche, à 25°C la fécondité des adultes survivants n'a pas été affectée par la maladie. Cette variabilité en fonction des conditions thermiques peut être liée à la diminution de l'effet-dose du champignon sur les larves à 25°C par rapport à 22°C. Par ailleurs, quelles que soient les conditions thermiques (22°C ou 25°C) on ne constate aucun changement significatif de la fertilité des oeufs pondus par les femelles survivantes. Les auteurs concluent que les effets secondaires de B. bassiana sur la fécondité du doryphore dépendent des conditions thermiques et qu'ils pourraient devenir négligeables à des températures élevées en plein champ.
    Notes: Abstract Secondary effects of the entomopathogenic hyphomycete Beauveria bassiana (Bals.) Vuill. were investigated on adults of Leptinotarsa decemlineata Say (Coleoptera:Chrysomelidae) treated as newly moulted 4th-instar larvae. Fecundity and egg hatching were monitored in surviving females mated with surviving males. When reared at 22°C, adult survivors showed a lessening of their reproductive potential during their whole life. According to the fungal inoculum dosage, the reductions of the total number of eggs laid per female and the mean number of eggs per egg mass ranged from 20% to 56% and from 18% to 46%, respectively. In contrast, at 25°C, the fecundity of survivors was not affected by the fungal infection. This variability of the secondary effects of B. bassiana according to temperature conditions might be related to the lower infection level of treated larvae at 25°C. Moreover, at both temperatures, eggs laid by females surviving fungal infection as larvae were as fertile as eggs laid by control insects. It is concluded that secondary effects of B. bassiana on the fecundity of the Colorado Potato Beetle are temperature-dependent and that they could become negligible at high temperatures under field conditions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of solution chemistry 20 (1991), S. 805-816 
    ISSN: 1572-8927
    Keywords: Alkanes ; 1-chlorobutane ; compressibilities ; excess volume ; expansibilities ; heat capacities ; heat of mixing ; liquids ; pressure ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Isothermal compressibilities KΥ and isobaric thermal expansion coefficients αp have been measured at 25 and 45°C for pure components and the following binary mixtures: 1-chlorobutane+normal alkanes (n-Cn) where n=6, 8, 10, 12, 14 and 16. With these results and other thermodynamic data from literature the next mixing quantities have also been reported: (∂V E/∂T)P, − (∂V)E/∂P)T, K S v , ∂H E/∂P)T, Δ(αpγVT and ΔCv. The obtained results have been compared at 25°C with the calculated values by using the Prigogine-Flory-Patterson theory of liquid mixtures. The theory predicts the excess volume VE and ∂V E/∂P)T values rather well, the C P E quite poorly, while for ∂V E/∂T)P and ∂V E/∂P)T it is only predicted the trend with the chain length of the n-alkane. The last two quantities show deviations between theoretical and experimental, slightly higher in systems with longer n-alkanes than for shorter ones. Our conclusion is that a nonrigid linear molecule, like 1-chlorobutane, has a low ability as a breaker of the pure n-Cn orientation correlations, in between that which we found for toluene and p-xylene and much smaller than for cyclohexane or benzene.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1572-879X
    Keywords: EXAFS ; Pt/Al2O3 catalyst ; temperature ; pressure ; carbon deposition ; carbon removal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract EXAFS has been used to follow in situ the structural evolution of a Pt/Al2O3 catalyst during removal of carbon by hydrogenation, after reduction and carbon deposition. After reduction at 350 °C, the total hydrogen pressure was raised to 3 atm. and n-heptane was injected over the sample. EXAFS measurements at the Pt edge were carried out with simultaneous on-line EXAFS analysis of the spectra. After observing the rapid formation of a carbon-platinum bond which is unmodified with time, we stopped the heptane flow, raised the temperature to 450 °C, and maintained hydrogen flow at 3 atm. The disappearance of the platinum-carbon bond during heat treatment in hydrogen was monitored via on-line analysis. No sintering of the metal particles was observed. EXAFS is thus proven to be an efficient tool to study not just structural changes of a catalyst during a hydrocarbon reaction [1], but also to use it as a technique to study other catalytic phenomena as well.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 60 (1991), S. 143-155 
    ISSN: 1570-7458
    Keywords: Thrips obscuratus ; Thysanoptera ; Thripidae ; New Zealand flower thrips ; rearing ; oviposition rate ; development time ; temperature ; diet ; pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The life history of New Zealand flower thrips (Thrips obscuratus (Crawford), Thysanoptera: Thripidae) was studied using a simple laboratory rearing method. The effects of temperature and diet on oviposition rate and development time were examined. Oviposition rate increased with increasing temperature between 10°C and 25°C. Development time for individual instars and for total development decreased with increasing temperature between 10°C and 27°C. Total development time ranged from 50 days at 10°C (female) to 10 days at 27°C (male). The relationship between temperature and development rate was expressed as a straight line such that lower thresholds of development of between 4.2°C and 6.3°C were established for life stages. Adult lifespan increased with decreasing temperature between 10°C and 25°C and females lived longer than males. At 10°C and 25°C females lived for an average of 34 and 3 weeks respectively. Thrips supplied with pollen exhibited highest and sustained levels of egg production in comparison to other diets. Larval mortality was lowest and development time fastest on diets of pollen and sucrose or fruit juice in comparison to other plant tissues. Larval development time was similar on four species of pollen.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1572-879X
    Keywords: EXAFS ; Pt/Al2O3 catalysts ; chlorine ; temperature ; pressure ; reduction ; deactivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract EXAFS has been used to follow in situ the structural evolution of a chlorinated and non-chlorinated Pt/Al2O3 catalyst during reduction in the temperature range of 300–500 °C. Smaller metal clusters are formed from the hydrogen reduction of the chlorinated catalyst, in contrast to the larger cluster formed from the non-chlorinated one. At 460 °C, the total hydrogen pressure was raised to 5 atm. and n-heptane was injected over the samples. EXAFS measurements at the Pt edge were carried out while hydrocarbon conversion was monitored with a gas Chromatograph. We observe the rapid formation of a carbon-platinum bond. This is unmodified while turnover rates and selectivities indicate evidence for deactivation. From this structural information supplied by EXAFS, correlated with the data obtained from gas chromatography, we find that our results are consistent with a model proposed by others where deactivation is due to the build-up of a multilayer of carbon.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 59 (1991), S. 59-66 
    ISSN: 1570-7458
    Keywords: Bruchidae ; adult polymorphism ; temperature ; hygrometric factors ; reproductive quiescence ; population dynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Callosobruchus maculatus (F.) est un Coléoptère Bruchidae tropical qui se développe aux dépens des graines de Vigna unguiculata. Les adultes présentent un polymorphisme; il y a en effet deux formes, la forme voilière et la forme non voilière, distinctes par des critères morphologiques, physiologiques et comportementaux. L'évolution des populations de C. maculatus a été suivie pendant toute la saison sèche et le début de la saison des pluies dans la région de Niamey au Niger (13° LN) à l'intérieur d'un grenier traditionnel. Pendant toute la saison sèche, 6 à 7 générations de C. maculatus de la forme non voilière se succèdent à l'intérieur du grenier. Les adultes de la forme voilière, très actifs, apparaissent surtout au cours de la saison des pluies lorsque la teneur en eau de l'atmosphère et la teneur en eau des graines augmentent. Les études expérimentales réalisées au laboratoire montrent que le polymorphisme imaginal est induit au cours du développement post-embryonnaire et dépend des conditions climatiques dans lesquelles celui-ci a lieu. Le taux d'adultes de la forme voilière est élevé lorsque les larves se développent dans des graines à forte teneur en eau (14 ou 15%). Lorsque la teneur en eau des graines est faible (6 à 7%), il n'y a émergence que d'adultes de la forme non voilière. Lorsque la teneur en eau des graines est comprise entre 12 et 13%, le taux d'adultes de la forme voilière s'accroit et varie avec la durée de la thermophase. A basse température il y a toujours émergence d'adultes de la forme non voilière, quelle que soit la teneur en eau des graines. L'influence de ces deux facteurs abiotiques sur l'induction du polymorphisme imaginal joue probablement un rôle important dans les mécanismes adaptatifs permettant le maintien de cette espèce en zone tropicale.
    Notes: Abstract Callosobruchus maculatus (F.) is a tropical insect (Coleoptera: Bruchidae) that develops in Vigna unguiculata (Walp) seeds. In this beetle, there are two distinct adult forms that differ in their morphology, physiology and behaviour, the flight form and the flightless form. The development of C. maculatus is analyzed in a traditional store in Niger. During the dry season, 6 to 7 generations of the flightless form develop in the store. When the atmospheric water content increases during the rainy season, adults of the flight form appear and escape from the store. Experimental studies have shown that the adult polymorphism is induced during post-embryonic development and depends on prevailing abiotic factors during this period. The proportion of flight from adults is high when larvae develop in moist seeds (14–15% of water content). Under dry conditions (6–7% of water content) only flightless adult forms emerge from the seeds. Temperatures during development also influence adult polymorphism. In thermoperiodic conditions with 12–13% seed water content, the proportion of flight from adults increases with increasing duration of the thermophase. However, at low temperatures, a high proportion of flightless adult forms emerge, regardless of seed water content. The adaptive importance of these two abiotic factors on polymorphism induction is examined in this study.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 7 (1991), S. 35-39 
    ISSN: 1476-5535
    Keywords: 2-Deoxy-d-glucose ; Yeast ; Catabolite repression ; Derepression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The non-metabolizable and toxic glucose analogue 2-deoxy-d-glucose (2-DOG) has been widely employed to screen for regulatory mutants which lack catabolite repression. A number of yeast mutants resistant to 2-DOG have recently been isolated in this laboratory. One such mutant, derived from aSaccharomyces cerevisiae haploid strain, was demonstrated to be derepressed for maltose, galactose and sucrose uptake. Furthermore, kinetic analysis of glucose transport suggested that the high affinity glucose transport system was also derepressed in the mutant strain. In addition, the mutant had an increased intracellular concentration of trehalose relative to the parental strain. These results indicate that the 2-DOG resistant mutant is defective in general glucose repression.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 19 (1991), S. 329-332 
    ISSN: 1432-0983
    Keywords: Mismatch repair ; Plasmid integration ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A single base pair mismatch (G:T or A:C) in the CYC1 gene of the integrative plasmid pAB218 stimulates up to a five-fold integration into the yeast chromosome. Analysis of chromosomal sites of plasmid integration suggests that the mismatch-stimulated integration is not targeted as would be expected if crossovers, localised in the region of the mismatch, were a necessary step in mismatch repair. Instead, the observed mismatch-stimulated plasmid integration could be due to potentially recombinogenic structures formed during mismatch repair, such as single-stranded gaps or denatured DNA regions extending around the plasmid molecule.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 12
    ISSN: 1432-0983
    Keywords: PET genes ; Yeast ; Mitochondria ; ATP synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This study details the characteristics of two temperature-conditional pet mutants of yeast, strains ts1860 and ts379, which at the non-permissive temperature show deficiencies in the formation of three mitochondrially encoded subunits of the ATP synthase complex. By analysis of mitochondrial translation products, and of mitochondrial transcription in temperature shift experiments from the permissive (22°C) to the non-permissive (36°C) temperature, it was concluded that the nuclear mutations in both mutants primarily inhibit synthesis of ATP synthase subunit 9, and that reductions in subunit 8 and 6 synthesis are secondary pleiotropic effects. Following transfer to 36°C, cells of mutant ts379 display a near complete inhibition of subunit 9 synthesis within 1 h, coincident with a marked reduction in the level of the cognate oli1 mRNA. On the other hand, near complete inhibition of subunit 9 synthesis in strain ts1860 occurs after 3 h at 36°C, at which time there is little change in the level of subunit 9 mRNA. In both mutants the mRNA levels for subunits 6 and 8 are not significantly affected at the time of inhibition of subunit 9 synthesis. Provision of an alternative source of subunit 8, translated extra-mitochondrially for import into the organelle, does not overcome the mutant phenotype of either mutant at 36°C, confirming that subunit 8 is not the sole or primary deficiency in each mutant. The mutants indicate that the products of a least two nuclear genes (designated AEP1 and AEP2) are required for the expression of the mitochondrial oli1 gene and the synthesis of subunit 9. The product of the AEP1 gene (defective in mutant ts1860) is required for translation of oli1 mRNA while the AEP2 product (defective in mutant ts379) is essential either for the stability of oli1 mRNA or for the correct processing of precursor transcripts to the mature message.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 19 (1991), S. 389-393 
    ISSN: 1432-0983
    Keywords: Yeast ; Pichia inositovora ; Linear plasmids ; Killer toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pichia inositovora, strain NRRL Y-18709, which contains three linear double-stranded DNA plasmids, pPinl-1, pPinl-2 and pPinl-3, was cured of these plasmids both by growing the strain in the presence of 50 μg/ml bisbenzimide, and by exposure to ultraviolet light. Both cured and uncured strains were tested for growth on a variety of carbon sources. No differences in growth response were detected, indicating no discernible involvement of the linear plasmids in the catabolism of these compounds. Culture supernatants of Pichia inositovora were shown to contain a substance larger than 100 kDa that is toxic to Saccharomyces cerevisiae, strain GS 1688. Toxin activity was optimal in YEPD assay plates containing 50 mM citrate buffer with a pH between 3.4 and 4.2. Culture supernatants from P. inositovora were also weakly active against Cephaloascus albidus, strain NRRL Y-18710, and Citeromyces matritensis, strain NRRL Y-18711. Concentrated supernatants from cured P. inositovora strains did not exhibit these activities, consistent with the hypothesis that this toxic activity is linear plasmid-encoded. Unlike the wellknown Kluyveromyces lactis system or the newly identified P. acaciae system, P. inositovora strains cured of their linear plasmids do not become detectably sensitive to toxin produced by the wild-type strain, suggesting a nonplasmid-encoded immunity function.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 14
    ISSN: 1432-0983
    Keywords: Yeast ; Molecular cloning ; Nitrogen mustard hyper-resistance ; Choline transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The recessive hnm1 mutant allele is responsible for hyper-resistance to nitrogen mustard in Saccharomyces cerevisiae. Transformation with a single-copy HNM1 wild-type allele of such hyper-resistant mutants will restore wild-type sensitivity to nitrogen mustard. By contrast the presence of multi-copy vectors containing HNM1, in either a hyper-resistant hnm1 mutant or an HNM1 wild-type, will lead to a novel, mustard-sensitive phenotype unrelated to defects in DNA repair genes. Gene disruption of HNM1 revealed that this gene is nonessential for cells prototrophic for choline (CHO1) but lethal for cells with a cho1 genotype. Sensitivity to nitrogen mustard of wild-type HNM1, but not of hnm1 mutants, depends on the choline content of the growth medium, with cells grown in choline-free medium exhibiting the highest sensitivity. Sequencing of a 300 bp DNA fragment of HNM1 revealed the identity of this gene with the CTR locus, which is responsible for choline transport in Saccharomyces cerevisiae.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 15
    ISSN: 1432-0983
    Keywords: Mutagen hyper-resistance ; Yeast ; Base sequence ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A multi-copy plasmid containing the SNQ3 gene confers hyper-resistance to 4-nitroquinoline-N-oxide (4NQO), Trenimon, MNNG, cycloheximide, and to sulfometuron methyl in yeast transformants. Restriction analysis, subcloning, and DNA sequencing revealed an open reading frame of 1950 bp on the SNQ3-containing insert DNA. Gene disruption and transplacement into chromosomal DNA yielded 4NQO-sensitive null mutants which were also more sensitive than the wild-type to Trenimon, cycloheximide, sulfometuron methyl, and MNNG. Hydropathic analysis showed that the SNQ3-encoded protein is most likely not membrane-bound, while the codon bias index points to low expression of the gene.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 19 (1991), S. 89-94 
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondria ; Intron ; Mobile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial and nuclear genomes of 21 yeast species belonging to 12 genera have been tested for the presence of sequences similar to seven S. cerevisiae mitochondrial introns (Sc cox1.1,2,3,4,5c, Sc cob.4 and Sc LSU.1) and one K. lactis mitochondrial intron (Kl cox1.2). Some introns, (Sc cox1.4, Sc cob.4, Sc LSU.1 and Kl cox1.2-all group I type), are widely distributed and are found in species with either basidiomycete or ascomycete affinities. This distribution is suggestive of recent sequence transfer between species. The remaining S. cerevisiae introns cross react with an additional species but with no set pattern. Pulsed field gel electrophoretic studies confirm that none of the tested mitochondrial introns cross react with nuclear DNA. These introns are, therefore, mitochondria-specific. Seven strains of K. lactis exhibit striking variability in intron content. In contrast to all mitochondrial introns tested, two introns of nuclear genes (the K. lactis actin gene and the S. cerevisiae RP29B gene) are not detected beyond their source species.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 17
    ISSN: 1432-0983
    Keywords: Yeast ; Pyruvate decarboxylase ; Gene expression ; Codon usage ; Gene fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three structural genes encode the pyruvate decarboxylase isoenzymes in the yeast Saccharomyces cerevisiae. PDC1 and PDC5 are active during glucose fermentation where PDC1 is expressed about six times more strongly than PDC5. Expression of PDC6 is weak and seems to be induced in ethanol medium. Consequently, pdc1Δ pdc5Δ double mutants do not ferment glucose and do not grow on glucose medium. Spontaneous mutants, derived from such a pdc1 pdc5 strain, were isolated which could again ferment glucose. They showed pyruvate decarboxylase activity due to a duplication of PDC6. The second copy of PDC6 was expressed under the control of the PDC1 promoter, which was still present in the pdc1 strain. However, the resulting PDC1-PDC6 fusion gene could only partially substitute for PDC1: to achieve normal growth and high pyruvate decarboxylase activity strains carrying PDC1-PDC6 required a functional PDC5 gene which is dispensable in a PDC1 wild-type background. Thus, expression of PDC5 depends on the state of the PDC1 locus: low in the PDC1 wild-type background and high in PDC1-PDC6 fusion strains and, as shown previously, in pdc1 mutants. The activation of PDC5 expression in PDC1-PDC6 strains may be due to particular properties of the PDC1-PDC6 fusion protein or simply to the weaker expression of PDC1-PDC6 in comparison to the wild-type PDC1 gene.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 18
    ISSN: 1432-0983
    Keywords: AEP2 ; Yeast ; Mitochondria ; ATP synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The temperature-conditional pet mutant, ts379, of Saccharomyces cerevisiae fails to synthesize mitochondrial ATP synthase subunit 9 at the restrictive temperature due to mutation of a single nuclear locus, AEP2. The inability to synthesize subunit 9 correlates with a lowered accumulation of the cognate oli1 mRNA indicating that the AEP2 product is involved in oli1 transcript maturation or stabilization. The AEP2 gene has been isolated in this study from a wild-type yeast genomic library by genetic complementation of ts379 at the restrictive temperature. A 1740 nucleotide open-reading frame was observed that encodes a basic, hydrophilic protein of 67534 Da which possesses a putative mitochondrial address signal. Disruption of chromosomal DNA within this reading frame produced a non-conditional respiratory mutant unable to synthesize subunit 9, identifying the AEP2 gene. Hybridization analyses indicate that AEP2 is located on chromosome XIII and produces a 2.1 kb poly(A)+ transcript. Two additional open-reading frames were found in close proximity to that of AEP2. The three open-reading frames shared no significant homology with entries in several data bases.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 19
    ISSN: 1432-0983
    Keywords: Pulsed field gel-electrophoresis ; S1 nuclease sensitive sites ; Repair ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Repair under non-growth conditions of DNA double-strand breaks (DSB) and chromatin sites sensitive to S1 endonuclease (SSS) induced by 60Cobalt-gamma rays were monitored in repair-competent and deficient strains of Saccharomyces cerevisiae by pulsed field gelelectrophoresis. In stationary-phase cells of a repair-competent RAD diploid, and an excision-deficient rad3-2 diploid, SSS are repaired as efficiently as DSB, whereas in a repair-competent RAD haploid, and a rad 50-1 diploid, neither SSS nor DSB are repaired. The rad18-2 diploid repairs DSB well but is defective in SSS repair. Obviously, SSS repair in yeast chromatin, like DSB repair, depends on recombination, but unlike DSB repair depends additionally on RAD18 function.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 20
    ISSN: 1432-0983
    Keywords: Yeast ; Mistranslation ; ψ-factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chromosomal omnipotent suppressor mutations recovered in ψ+ strains of Saccharomyces cerevisiae were brought into ψ− cytoplasm. SUP46, SUP138 and SUP139 acted as dominant omnipotent suppressors in the ψ− cytoplasm though their suppressor activity was substantially reduced. SUP46 and SUP138 conferred recessive thermosensitivity and antibiotic sensitivity in ψ− cytoplasm as in ψ+ cytoplasm. On the other hand, sup111 through sup115, which acted as recessive omnipotent suppressors in the ψ+ cytoplasm, manifested no, or very low, suppressor activity in the ψ− cytoplasm. They, however, still enhanced the efficiency of the SUP29 tRNA suppressor in ψ− cytoplasm. A multicopy plasmid carrying the wild-type SUP35 gene enhanced the efficiency of sup111 in ψ− cytoplasm.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 21
    ISSN: 1432-0983
    Keywords: Yeast ; Mutant ; p-Fluoro-dl-phenylalanine ; β-Phenethyl-alcohol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary p-Fluoro-dl-phenylalanine (PFP)-resistant mutants which produce a large amount of β-phenethyl-alcohol, a rose-like flavor component, were isolated from the isogenic strains X2180-1A and X2180-1B of Saccharomyces cerevisiae. Cells of these mutants accumulated phenylalanine and tryptophan more than 3-fold times that of wild-type cells, while they accumulated less than half the tyrosine. The activity of prephenate dehydrogenase (PDG) (EC 1.3.1.12) was markedly decreased while that of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15) was increased. Genetic analysis revealed that the mutation occurred at the TYR1 locus, encoding PDG, and that the mutated TYR1 gene, tyr1-pfp, caused both PFP resistance and β-phenethyl-alcohol overproduction. This was supported by molecular genetic studies with cloned tyr1-pfp DNA.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 22
    ISSN: 1432-0983
    Keywords: Peptides ; Transport ; Regulation ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The transport of small peptides into the yeast Saccharomyces cerevisiae is subject to complex regulatory control. In an effort to determine the number, and to address the function, of the components involved in peptide transport and its regulation, spontaneous mutants resistant to toxic di- and tripeptides were isolated under inducing conditions. Twenty-four mutant strains were characterized in detail and fell into two phenotypic groups; one group deficient in amino acid-inducible peptide uptake, the other with a pleiotropic phenotype including a loss of peptide transport. Complementation analysis of recessive mutations in 12 of these strains defĩned three groups; ptr1 (nine strains), ptr2 (two strains), and ptr3 (one strain). Isolation and screening of 31 additional N-methyl-N-nitro-N-Nitrosoguanidine (MNNG)-induced, peptide transport-deficient mutants produced one ptr3 and 30 ptr2 strains: no additional complementation groups were detected. Uptake of radiolabeled dileucine was negligible in ptr1 and ptr2 strains and was reduced by 65% and 90% in the two ptr3 mutants, indicating that all strains were defective at the transport step. We conclude that the S. cerevisiae amino acid-inducible peptide transport system recognizes a broad spectrum of peptide substrates and involves at least three components. One gene, PTR3, may play an indirect or regulatory role since mutations in this gene cause a pleiotropic phenotype.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 23
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 32 (1991), S. 396-404 
    ISSN: 1432-1432
    Keywords: Yeast ; Mitochondrial DNA ; Polymirphism ; Repeated sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A spontaneously arising mitochondrial DNA (mtDNA) variant ofSaccharomyces cerevisiae has been formed by two exta copies of a 14-bp sequence (TTAATTAAATTATC) being added to a tandem repeat of this unit. Similar polymorphisms in tandemly repeated sequences have been found in a comparison between mtDNAs from our strain and others. In 5850 bp of intergenic mtDNA squence, polymorphisms in tandemly repeated sequences of three or more base pairs occur approximately every 400–500 bp whereas differences in 1–2 bp occur approximately every 60 bp. Some polymorphisms are associated wit optional G+C-rich sequences (GC clusters). Two such optional GC clusters and one A+T repeat polymorphism have been discovered in the tRNA synthesis locus. In addition, the variable presence of large open reading frames are documented and mechanisms for generating intergenic sequence diversity inS. cerevisiae mtDNA are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 24
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 32 (1991), S. 439-442 
    ISSN: 1432-1432
    Keywords: Yeast ; Mitochondrial DNA ; ori ; rep ; Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Threeori elements (ori 2,ori 5, andori 7) have been sequenced inSaccharomyces cerevisiae strain Dip 2 and compared to the equivalentori elements of a second strain (B). Bothori 2 andori 5 exhibit 98% base matching between strains Dip 2 and B. In contrast, the thirdori element (ori 7) exhibits extensive sequence rearrangements whereby a segment located downstream in the consensus strain occurs within theori structure in Dip 2. This represents a novel polymorphic form of the yeast mitochondrial genome.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 25
    ISSN: 1573-0832
    Keywords: Fusarium crookwellense ; temperature ; zearalenone ; a-zearalenol ; nivalenol ; mycotoxins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract After 6 weeks incubation on rice 2 strains of Fusarium crookwellense produced more zearalenone (6060–5010 mg/kg dry wt of culture) at ambient temperature (16–29°C) in daylight than at ambient temperature (18–23 °C) in darkness or at controlled temperatures of 11 °C, 20 °C or 25 °C in darkness. Yields at 25 °C were low. Incubation at 11 °C during the second 3 weeks incubation increased yields only when preliminary incubation had been at 25 °C. After 6 weeks incubation at controlled temperatures in darkness, 4 strains produced most zearalenone at 20 °C (2460-21 360 mg/kg), 1 strain at 11 °C (6570 mg/kg). Yields at a temperature oscillating daily from 10–20 °C were less than at 15 °C. One of the 5 strains produced appreciable amounts of a-zearalenol (1645 mg/kg at 20°C) and 2 of nivalenol (340 and 499 mg/kg at 20 °C).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 26
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 3 (1982), S. 191-292 
    ISSN: 1573-0867
    Keywords: spinach ; NO3-content ; ionic balance ; plant age ; season of growth ; soil moisture ; soil pH ; Mo-spraying ; light intensity ; temperature ; K-dressing ; P-dressing ; varietal differences ; N-dressing ; plant-available N ; N-form ; NO3: NH4-ratio ; nitrification ; inhibitors ; type of N-carrier ; time of application of N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Environmental factors and cultural measures affecting the NO3-content in spinach were studied indoors, in water-, sand- and soil-culture experiments. In the field, the influences of variations in N-fertilizing practices and in spinach varieties were also tested. High NO3-contents in spinach were found with low light intensities, with low soil-moisture contents, and with high temperatures. NO3-contents increased with increasing K-dressing (less so with KCl than with K2SO4), but decreased with increasing soil pH. In pot experiments, positive results were obtained with sulphur-coated urea, with farmyard manure and with pigmanure slurry. Application of Mo as a spray onto spinach leaves, and variations in P-dressings and in soil P-status were found not to affect the NO3-content in spinach. In pot experiments, NO3-contents decreased with progressing plant age (in autumn less so than in spring). Within spinach plants, NO3-contents were highest in petioles and older leaves. Varietal differences in NO3-contents were observed in a pot- and a field experiment. In pot- and field experiments, partial or complete replacement of NO3-N by NH4-N in general caused the NO3-content in spinach to decrease. However, such a replacement was shown not always to result in lower NO3-contents. Additional factors involved are e.g. the use and effectiveness of nitrification inhibitors, the soil type and the amount of available N. The amount of N added and, in the field, the amount of N available in the soil before sowing, strongly affected the NO3-content in spinach. Under field conditions, nitrogen appeared to be taken up from the top 60 cm of the soil profile. The effects of variations in timing of nitrogen applications were absent in a pot experiment and not consistent in field experiments.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 27
    Electronic Resource
    Electronic Resource
    Springer
    Landscape ecology 6 (1991), S. 15-27 
    ISSN: 1572-9761
    Keywords: Climate ; microclimate ; energy balance ; dunes ; dune formation ; wind ; temperature ; precipitation ; evaporation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extremely important to the climate in any region are the radiation balance and the exchange processes of heat, water vapour and momentum. Most climatological parameters (e.g. temperature, humidity, wind speed, cloudiness and precipitation) are the direct or indirect result of the radiation balance and these exchange processes. The weather of the West European coast from Tarifa (Spain) to Skagen (Denmark) is especially suitable for the formation of dunes. Often a wind is blowing, varying widely in force and direction. The conditions are optimal for the formation of high and wide dune complexes, given a large supply of sand by the sea. The annual precipitation surplus is considerable for most of this coast. This favours the establishment of vegetation, and thereby it enhances dune formation. The short distance to the land-sea border causes strong gradients in several climatological parameters. These gradients lead to mesoscale effects, such as land-sea breezes and coastal fronts. The varying vegetation cover and the presence of slopes in all directions induce a strongly varying microclimate. However, this microclimate is not unique to the coastal dunes. Unique is the interaction with the wide range of ambient weather, which is inherent to the coast. It is not possible to be conclusive about the effects of climatic change on coastal dunes because climate models are not yet able to predict the changes adequately and because these models supply information on the expected mean climate, but not on the actual weather.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 28
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 20 (1991), S. 471-474 
    ISSN: 1432-0983
    Keywords: Yeast ; DNA replication ; Chemical mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Incubation of cdc8 mutants of the yeast Saccharomyces cerevisiae in YPD under permissive conditions, when DNA replication is taking place, prior to transfer to restrictive conditions, strongly stimulates induction of cdc + colonies of ethyl methane sulphonate (EMS)- and methyl methane sulphonate (MMS)-treated yeast strains HB23 (cdc8-1/cdc8-3), HB26 (cdc8-3/cdc8-3) and HB7 (cdc8-1/cdc8-1). After diepoxybutane (DEB) treatment, both the induction of cdc + colonies and their stimulation after incubation in YPD under permissive conditions is low. The results obtained show that stimulation of induction of cdc + colonies under permissive conditions occurs not only after UV-treatment, but also after treatment with such mutagens as EMS and MMS.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 29
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial DNA ; Antibiotic resistance mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A large proportion of the spontaneous erythromycin resistant mutants isolated from a strain carrying a previously-induced chloramphenicol resistance mutation at cap3 do not map at ery1, the locus most often associated with mitochondrial erythromycin resistance. Most of the new mutations are also nonallelic at spil, spi2, and other known antibiotic resistance loci within the 21S rRNA gene; they are allelic with each other and define the new locus, ery2. Induced second-site erythromycin resistant mutants from the cap r3 strain, as well as spontaneous or induced mutants from strains carrying a cap r 1 mutation, all tend to map at eryl. The cap r3 mutation is apparently necessary for the expression of erythromycin resistance resulting from a second mutation at ery2.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 30
    ISSN: 1432-0983
    Keywords: Yeast ; Thermoconditional DNA repair ; Mutagenesis ; Allelism test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Of two mutant genes (snm1-2 ts and snm2-1 ts) conferring thermoconditional mutagen sensitivity in Saccharomyces cerevisiae one (snm2-1 ts) is shown to be centromere-linked. At the restrictive temperature this allele reduces UV-induced back mutation frequency of the ochre allele hiss-2 but has no influence on forward mutation at the CAN1 locus. Complementation tests and recombination analysis revealed snm2 ts to be allelic with rad5 (rev2).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 31
    ISSN: 1432-0983
    Keywords: Electro-fusion ; Yeast ; Plasmogamy ; Proliferation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Electric field-induced fusion was induced between Saccharomyces cerevisiae protoplasts from the ρ − heterozygous diploid strain 2114 and the respiratory-competent diploid strain 3441, carrying chromosomal markers. Close membrane contact between the cells of the two different strains (ratio 1:1) was achieved by dielectrophoresis in a weak inhomogeneous alternating field (about 1 kV/cm, 2 MHz). Due to dielectrophoresis pearl chains of two or more cells of the two strains are formed between the electrodes. Cell fusion was induced by application of two single square field pulses sufficiently high to induce reversible electrical breakdown in the membrane contact zone between cells within a pearl chain (about 7 to 8 kV/cm field strength and 40 Ms duration). The two subsequent pulses were applied at an interval of about 10 s. Hybrids could be isolated on selection medium in a high yield (compared with conventional fusion techniques). The hybrids were diploid, respiratory-competent and produced prototrophic spores. Thus, the fused hybrids contained only the chromosomal markers of strain 2114 and the cytoplasmic marker for respiratory competence from strain 3441; electro-fusion thus resulted mainly in plasmogamy.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 32
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 6 (1982), S. 93-98 
    ISSN: 1432-0983
    Keywords: Yeast ; Genetic mapping ; Trisomic analysis ; Arginine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary By use of a set of 8 aneuploid strains of the yeast Saccharomyces cerevisiae, carrying from 1 to 5 identified disomic chromosomes, in crosses to a set of haploid strains collectively bearing 11 unmapped genes, the following chromosome assignments were obtained for these unmapped genes: arg80 on XIII;arg3 on X;car2 on XII; cpa1 and tsm8740 on XV; tsm7269 (=rna6) on II; cpa2 on X or XV; arg82 and tsm4572 on III, IV or XVI; car1 and arg81 on II, IV, VI, VII or XVI. Linkage tests between the unmapped genes and markers located on the chromosomes that had been designated as possible carriers by the previous analysis allowed 8 genes to be localized. The remaining three genes, cpa2, car1 and arg81 (located on fragment F8), could not be positioned on any of the chromosomes indicated by the trisomic analysis, in spite of testing for linkage to markers covering most of the known regions of these chromosomes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 33
    ISSN: 1432-0983
    Keywords: Petite mutation ; NUC2 nuclease ; Yeast ; RAD52 ; Ethidium bromide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Defects in the RAD52 gene of the yeast Saccharomyces cerevisiae reduce the levels of the NUC2 endo-exonuclease by approximately 90% compared to the levels in wild-type strains. To examine the potential role of this nuclease in the induction of mitochondrial ‘petite’ mutations, congenic RAD52 and rad52-1 haploids were subjected to treatment with ethidium bromide, a well-known inducer of these mutations. The rad52 strain showed a much higher resistance to ethidium bromide-induced petite formation than the corresponding wild-type strain. Two approaches were taken to confirm that this finding reflected the nuclease deficiency, and not some other effect attributable to the rad52-1 mutation. First, a multicopy plasmid (YEp213-10) carrying NUC2 was transformed into a RAD52 strain. This resulted in an increased fraction of spontaneous petite mutations relative to that seen for the same strain without the plasmid and sensitized the strain carrying the plasmid to peptite induction by ethidium bromide treatment. Second, a strain having a nuc2 allele that encodes a temperaturesensitive nuclease was treated with ethidium bromide at the restrictive and permissive temperatures. Petite induction was reduced under restrictive conditions. Enzyme assays revealed that the RAD52 (YEp213-10) strain had the highest level of antibody-precipitable NUC2 endo-exonuclease whereas the nuc2 and rad52 mutants had the lowest levels. Furthermore, addition of ethidium bromide to the reaction mixture stimulated the activity of the nuclease on double-stranded DNA. Peptite induction by antifolate-mediated thymine nucleotide depletion was also inhibited by inactivation of RAD52 indicating that the effect of reduced NUC2 endo-exonuclease was not restricted to ethidium bromide treatment. Taken collectively, these results indicate that the NUC2 gene product functions in the production of mitochondrial petite mutations.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 34
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 5 (1982), S. 153-155 
    ISSN: 1432-0983
    Keywords: Yeast ; Mutant cell-wall ; Permeability exponentialy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When Saccharomyces cerevisiae SY15 rho° mutant cells grown in media stabilized with 10% sorbitol were suspended in 2% sorbitol solutions, 60–70% of the population did not lyse and became permeable to native high molecular weight DNA. Maximal incorporation of DNA to DNase resistant state was measured after 60 min of incubation in presence of 5 μg/ml DNA and 10 mM CaCl2. These results suggest that the fragile mutants might be tested as hosts for transformation of whole yeast cells.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 35
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 20 (1991), S. 1-3 
    ISSN: 1432-0983
    Keywords: Yeast ; Transformation ; Ethanol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A technique is described in which ethanol is used to improve the genetic transformation of intact yeast (Saccharomyces cerevisiae) cells pretreated with LiAc and PEG. Transformation efficiency was increased with increasing concentrations of ethanol with a peak at 10% concentration. The effect varies with different yeast strains and plasmids and up to a maximum of a 15-fold increase was observed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 36
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 20 (1991), S. 25-31 
    ISSN: 1432-0983
    Keywords: Yeast ; TSM1 sequence ; Essential gene ; MAT distal cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have cloned the region from MAT to THR4 on chromosome III of Saccharomyces cerevisiae. Although the region is only 15 kb, the two loci are genetically separated by 22 cM. This is in sharp contrast to the very low level of recombination (2 cM in 22 kb) that is observed in the adjacent CRY1-MAT interval, and suggests that there may be a “hot spot” for recombination in the MAT-THR4 region. The DNA sequence of the first 4.4 kb distal to MAT reveals an open reading frame that we have identified as the essential gene, TSM1. Surprisingly, the TSM1 open reading frame of 1 410 amino acids extends into the MAT locus, such that the 3′-end of the MATα1 transcript ends 15 bp from the 3′-end of the TSM1 open reading frame.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 37
    ISSN: 1432-0983
    Keywords: Yeast ; Enhancer ; Transcriptional elements ; Transcriptional factors ; Regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Though highly complex enhancers found in animal cells have not been reported to occur in yeasts they are able to activate the transcription of adjacent genes in yeast cells. Saccharomyces cerevisiae expresses a large number of nuclear proteins that are able to recognize, and specifically bind to, the enhancer sequences of the SV40 animal tumor virus. The complexity of proteins that interact with different elements of the animal enhancers is similar in yeast and animal cell nuclear extracts. Most enhancer motifs, recognized by known trans-acting factors, are protected in footprinting experiments by yeast nuclear proteins.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 38
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Ergosterol ; Squalene synthetase ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ERG9 gene of Saccharomyces cerevisiae has been cloned by complementation of the erg9-1 mutation which affects squalene synthetase. From the 5kkb insert isolated, the functional gene has been localized on a DNA fragment of 2.5 kb. The presence of squalene synthetase activity in E. coli bearing the yeast DNA fragment isolated, indicates that the structural gene encoding squalene synthetase has been cloned. The sequence of the 2.5 kb fragment contains an open reading frame which could encode a protein of 444 amino acids with a deduced relative molecular mass of 51 600. The amino acid sequence reveals one to four potential transmembrane domains with a hydrophobic segment in the C-terminal region. The N-terminus of the deduced protein strongly resembles the signal sequence of yeast invertase suggesting a specific mechanism of integration into the membranes of the endoplasmic reticulum.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 39
    ISSN: 1432-0983
    Keywords: Mitochondria ; Intron ; Telomere ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The junctions between X and Y′ subtelomeric repeats in Saccharomyces cerevisiae usually contain a stretch of telomere sequences, (G1–3T)n. Two of three cloned X-Y′ junctions from strain YP1 have a replacement of about 200 bp of X, the internal telomere sequence, and 49 bp of Y′ by a 292 bp sequence. The first 227 bp of this insertion sequence are 100% identical to the fourth intron of cytochrome b. The rest of the insertion has homology to an unknown dispersed nuclear sequence. Recombination among subtelomeric regions can explain the nuclear distribution of this sequence and why telomeres can trap and maintain sequences that would otherwise be lost.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 40
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 6 (1982), S. 99-103 
    ISSN: 1432-0983
    Keywords: Ultraviolet light mutagenesis ; Mitochondrial genome ; Meiosis ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Clones derived from ascospores from ultraviolet irradiated diploid cells were examined for the genetic determinants or respiratory properties. Approximately 10% of the cells produced petites of mitochondrial origin at the dose applied. Among 13 asci which produced mitochondrial petites with high frequencies, 6 asci of uniparental type, 0 grandes : 4 petites, were observed. Furthermore, most of the petite spore clones from each individual uniparental ascus showed similar levels of suppressiveness and of mitochondrial gene retention. From these results it is suggested that a single mitochondrial genome participates meiosis in yeast.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 41
    ISSN: 1432-0983
    Keywords: In vitro mutagenesis ; PET-genes ; RNA-leader ; Ribosomal scanning ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report that the major transcription start sites of the yeast PET gene SCO1 are located at positions-149 and -125 relative to the AUG initiation codon of the SCO1 reading frame. The leader sequences of the resulting mRNAs possess a single AUG codon at position-49, which initiates a short open reading frame of three amino acids. The recent finding of a similar situation in the case of the PET gene CBS1 prompted us to address the question as to whether these AUG codons might play some role in the expression of these PET genes. After removal of the upstream AUG codons by site-directed mutagenesis, expression was monitored by use of lacZ fusions and compared to the respective wildtype constructs. Our data show that under all growth conditions tested the leader-contained AUG initiation codons have no significant influence on the expression of both PET genes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 42
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 6 (1982), S. 179-188 
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondria ; var1 gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Several mit mutants mapping within or near the var1 determinant region have been characterized genetically and biochemically. These mutants were isolated using a new enrichment protocol which simplifies the isolation and identification of rare respiration-deficient mutants of yeast. Two of the mutants, PZ200L and PZ206, map in genome segments which flank the known varl gene reading frame; nevertheless, both belong to the same complementation group, apparently that of the varl gene. A third mutant, PZ200R is closely linked to one of the varl allelic determinants now known to be a short insertion within the gene. All three var1 mutants exhibit decreased levels of mitochondrial protein synthesis and negligible activity of the respiratory enzyme complexes. Another cluster of mutants belonging to a separate complementation group from that defined by PZ200L and PZ206 was also mapped and it contains mutants in the nearby serine tRNA gene. The isolation of these mutants in the varl region shows that the varl locus contains information essential for the maintenance of respiration-competent mitochondria. Because these mutants affect mitochondrial protein synthesis, their existence supports the previous hypothesis that the varl protein is an integral component of mitochondrial ribosomes. Furthermore, the mutant sites are present in a DNA sequence that is highly, rich in A+T residues that also contains a gene. Since approximately 50% of the yeast mitochondrial genome is similarly rich in A+T and since most of those regions have not yet been sequenced it is quite possible that other A+T-rich genes may exist.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 43
    ISSN: 1432-0983
    Keywords: Nitrosoguanidine ; Comutation ; Yeast ; Chromosome replication pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Contrary to what happens in bacteria, mutations induced by nitrosoguanidine in yeast are not accompanied by an excess of mutations in nearby genes. We have investigated nitrosoguanidine mutagenesis in three regions of the yeast genome: the contiguous DNA segments HIS4A, HIS4B and HIS4C, located on chromosome III; ADE1 and CDC15 separated by about 3 map units on chromosome I; and CAN1, some 50 map units away from the centromere on chromosome V. Revertants at HIS4C never suffered mutations at HIS4A or HIS4B. Reversion at CDC15 did not affect the frequency of mutation at ADE1. No tsm mutations, leading to thermonsensitivity, were found in the immediate vicinity of the locus CAN1 after selecting for canavanine resistant mutants. However, as expected from nitrosoguanidine mutagenesis of replication points and the fixed pattern of chromosome replication, the induced tsm mutations seem not to map randomly over the yeast genome; in fact, two out of the three groups of such tsm mutations studied are located in the same chromosome arm as CAN1, indicating that these two regions are replicated at the same time as CAN1. Replication synchrony is less than perfect, since the tsm mutations of each group affect many different genes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 44
    ISSN: 1432-0983
    Keywords: Yeast ; Cycloheximide ; Ribosomal mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary At least four different mutations at the cyh2 locus (rp1X; gene product: YL24) of Saccharomyces cerevisiae confer cycloheximide resistance. The mutant YL24 proteins are either more basic (high-level resistant phenotype), more acidic (low-level resistant phenotype), or unchanged in their electrophoretic mobility (both low-and high-level resistant phenotypes). None of the mutations at other loci seem to induce high-level resistance to cycloheximide.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 45
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 5 (1982), S. 171-180 
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial genes ; Vegetative segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A three-factor cross of Saccharomyces cerevisiae involving the cap1, ery1, and oli1 loci was done, with partial pedigree analyses of 117 zygotes. First, second, and third buds were removed and the genotypes of their diploid progeny determined, along with those of the residual zygote mother cell. Results were analyzed in terms of frequencies of individual alleles and of recombinant genotypes in the dividing cells. There is a gradual increase in the frequency of homoplasmic cells and in gene frequency variance during these three generations, as would result from stochastic partitioning of mtDNA molecules between mother and bud, probably coupled with random drift of gene frequencies in interphase cells. These phenomena are more pronounced for buds than for mothers, suggesting that buds receive a smaller sample of molecules. End buds are more likely to be homoplasmic and have a lower frequency of recombinant genotypes than do central buds; an end bud is particularly enriched in alleles contributed by the parent that formed that end of the zygote. Zygotes with first central buds produce clones with a higher recombination frequency than do those with first end buds. These results confirm previous studies and suggest that mixing of parental genotypes occurs first in the center of the zygote. If segregation were strictly random, the number of segregating units would have to be much smaller than the number of mtDNA molecules in the zygote. On the other hand, there is no evidence for a region of the molecule (“attachment point”) which segregates deterministically.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 46
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 6 (1982), S. 29-30 
    ISSN: 1432-0983
    Keywords: Yeast ; Plasmid ; Repair ; Ligase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We show that the DNA ligase encoded or controlled by the cdc9 gene in Saccharomyces cerevisiae is required for replication of plasmid DNA but that excision repair of pyrimidine dimers in plasmid DNA can be completed in its absence.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 47
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 6 (1982), S. 195-201 
    ISSN: 1432-0983
    Keywords: Benomyl resistance ; Yeast ; Mitosis ; Cell cycle mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated 150 benomyl resistant mutants of the fission yeast Schizosaccharomyces pombe. Seven of these mutants were found to be cold sensitive for mitosis. These mutants were the subject of physiological, cytological and genetical characterisation. Growth and division of the seven mutants were similar to the wild type strain at 35 °C. After shift from the permissive (35 °C) to the restrictive temperature (20 °C) the mutants became blocked in mitosis whilst cellular growth continued. Consequently, elongate cells were formed. Six of the seven benomyl resistant mutants became blocked in mitosis at 20 °C with a single aberrant nucleus. In every case the benomyl resistant and cold sensitive phenotype was due to a mutation in a single nuclear gene. These mutants were found to comprise a single genetic linkage group (ben4) and were unlinked to existing TBZ/MBC resistant mutants of S. pombe. Whilst no cross resistance was found in our mutants to TBZ, six of the seven mutants were super sensitive to the spindle poison CIPC. We believe that the phenotype exhibited by these mutants is consistent with a defective tubulin subunit.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 48
    ISSN: 1432-072X
    Keywords: Ions ; Concentration ; Regulation ; Cytoplasm ; Vacuole ; Yeast ; Saccharomyces carlsbergensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Intracellular distributions of K+, Mg2+ and orthophosphate under various conditions of cultivation or incubation of the yeast Saccharomyces carlsbergensis were studied by differential extraction of ion pools. The decisive role of vacuolar compartmentation of ions in regulation of K+, Mg2+ and orthophosphate levels in the yeast cytoplasm was shown. The content of intracellular K+ and Mg2+ in yeast increased or decreased primarily depending on the increase or decrease in the vacuolar ion pool. The levels of K+ and Mg2+ in the cytoplasm were practically unchanged. Vacuoles were involved in regulation of Mn2+ concentration in the cytoplasm of the yeast S. carlsbergensis accumulating this ion in the presence of glucose. Alongside the vacuolar compartmentation, the chemical compartmentation, i. e. formation of bound Mg2+, Mn2+ and K+ was, evidently, also involved in the control of ion levels in the cytoplasm. The orthophosphate level in the yeast cytoplasm was regulated by its accumulation in vacuoles and biosynthesis of inorganic polyphosphates in these organelles. The biosynthesis of low-molecular weight polyphosphates occurred parallel to the accumulation of Mg2+ or Mn2+ in vacuoles, thus confirming the availability of the other mechanism for the transport of these ions through the tonoplast differing from the transport mechanism through the plasmalemma.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 49
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 131 (1982), S. 298-301 
    ISSN: 1432-072X
    Keywords: Yeast ; Protoplast ; Compartmentation ; Vacuole ; Trehalose ; Trehalase ; Carbohydrate metabolism ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts of Saccharomyces cerevisiae synthesized and degraded trehalose when they were incubated in a medium containing traces of glucose and acetate. Such protoplasts were gently lyzed by the polybase method and a particulate and soluble fraction was prepared. Trehalose was found in the soluble fraction and the trehalase activity mostly in the particulate fraction which also contained the vacuoles besides other cell organelles. Upon purification of the vacuoles, by density gradient centrifugation, the specific activity of trehalase increased parallel to the specific content of vacuolar markers. This indicates that trehalose is located in the cytosol and trehalase in the vacuole. It is suggested that trehalose, in addition to its role as a reserve may also function as a protective agent to maintain the cytosolic structure under conditions of stress.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 50
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 132 (1982), S. 144-148 
    ISSN: 1432-072X
    Keywords: Yeast ; Cell wall ; Mannoproteins ; Envelope turnover ; Concanavalin A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By pulse and chase labeling experiments, two independent mannoprotein pools have been found associated with the Saccharomyces cerevisiae envelope. One of them probably corresponds to mannoproteins localized in the periplasmic space. These molecules showed a high turnover rate at 28° C. The second pool is formed by intrinsic wall mannoproteins which are apparently stable for long periods of time, after a small initial turnover. These results suggest that at least part of the mannoproteins initially found in the periplasmic space may move into the wall. The time lag between the addition of the radioactive precursors and their incorporation in the cell envelope (20–30 min for amino acids and about 10 min for carbohydrate) indicates that protein formation and carbohydrate incorporation take place in succession. Moreover, bulk glycosylation of mannoproteins seems to occur close in time to the moment of secretion into the periplasmic space.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 51
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 133 (1982), S. 155-161 
    ISSN: 1432-072X
    Keywords: Microtubule ; Nocodazole ; Yeast ; Cell cycle ; Dimorphism ; Fungus ; Wangiella dermatitidis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The microtubule inhibitor nocodazole {methyl-5-[2-(thienylcarbonyl)-1H-benzimidazol-2-yl]-carbamate} prevented nuclear migration and nuclear division in yeasts and developing multicellular forms of the polymorphic fungus Wangiella dermatitidis. It did not prevent yeast bud formation during at least two or three budding cycles, and caused yeasts to accumulate as premitotic forms with one to three buds. The effects of the drug suggested that at least three control pathways were involved in the yeast cell cycle; that the nocodazole block point was separate from the execution points of two temperature-sensitive mutations which lead to multicellularity; and that microtubules were controlling neither the yeast budding process nor the development of multicellular forms.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 52
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 133 (1982), S. 131-136 
    ISSN: 1432-072X
    Keywords: Fructose-bisphosphatase deficient mutants ; Yeast ; Schizosaccharomyces pombe ; Gluconeogenesis ; Glucose repression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We showed that in the yeast Schizosaccharomyces pombe, fructose-bisphosphatase is not subject to catabolite inactivation as it was observed in Saccharomyces cerevisiae. However, this enzyme activity is sensitive to catabolite repression in both yeasts. Two mutants lacking completely fructose-bisphosphatase activity were found. They were unable to grow on glycerol medium. They were still respiratory competent and exhibited the ability to derepress partially malate dehydrogenase activity. In glucose exponential phase culture, the parental strain lacks completely the fructosebisphosphatase activity due to catabolite repression. In these conditions, the growth is slowed down only in the mutants eventhough both mutants and their parental strain lack this enzyme activity. Normal sporulation and poor spore germination were observed for one mutant whereas, only in the presence of glucose, normal sporulation and normal spore germination were observed for the second mutant. Mendelian segregation of glycerol growth was found for the well germinating mutant. It is of nuclear heredity. The two mutations appeared to be closely linked.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 53
    ISSN: 1573-4927
    Keywords: alcohol dehydrogenase ; Drosophila ; selection ; ethanol ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Drosophila melanogaster larvae were subjected to 10 generations of selection on 6% ethanol at 17, 25, and 30°C. For each temperature there was a significant (P〈0.01) increase in the frequency of the Adh isoallele. Controls with no ethanol showed no change in the frequency of the Adh F isoallele. Larvae subjected to stronger selection on 8% ethanol confirmed the results. When adults of various ages were subjected to 16 and 32°C, the ADHF isoenzyme retained its twofold advantage in activity over ADHS regardless of the temperature. The same result was obtained with larvae at 16 and 35°C. Although some effect of temperature was demonstrated, it was concluded that the effect was not strong enough for temperature to be a selective factor under the conditions studied. However, ethanol is a strong selective factor for laboratory populations.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 54
    ISSN: 1432-1424
    Keywords: skeletal muscle ; Cl− efflux ; Cl− channel ; pH ; muscle membrane ; temperature ; diethylpyrocarbonate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Efflux of36Cl− from frog sartorius muscles equilibrated in two depolarizing solutions was measured. Cl− efflux consists of a component present at low pH and a pH-dependent component which increases as external pH increases. For temperatures between 0 and 20°C, the measured activation energy is 7.5 kcal/mol for Cl− efflux at pH 5 and 12.6 kcal/mol for the pH-dependent Cl− efflux. The pH-dependent Cl− efflux can be described by the relationu=1/(1+10n(pK a -pH)), whereu is the Cl− efflux increment obtained on stepping from pH 5 to the test pH, normalized with respect to the increment obtained on stepping from pH 5 to 8.5 or 9.0. For muscles equilibrated in solutions containing 150mm KCl plus 120mm NaCl (internal potential about −15 mV), the apparent pK a is 6.5 at both 0 and 20°C, andn=2.5 for 0°C and 1.5 for 20°C. For muscles equilibrated in solutions containing 7.5mm KCl plus 120mm NaCl (internal potential about −65 mV), the apparent pK a at 0°C is 6.9 andn is 1.5. The voltage dependence of the apparent pK a suggests that the critical pH-sensitive moiety producing the pH-dependent Cl− efflux is sensitive to the membrane electric field, while the insensitivity to temperature suggests that the apparent heat of ionization of this moiety is zero. The fact thatn is greater than 1 suggests that cooperativity between pH-sensitive moieties is involved in determining the Cl− efflux increment on raising external pH. The histidine-modifying reagent diethylpyrocarbonate (DEPC) applied at pH 6 reduces the pH-dependent Cl− efflux according to the relation, efflux=exp(−k·[DEPC]·t), wheret is the exposure time (min) to DEPC at a prepared initial concentration of [DEPC] (mm). At 17°C,k −1=188mm·min. For temperatures between 10 and 23°C,k has an apparent Q10 of 2.5. The Cl− efflux inhibitor SCN− at a concentration of 20mm substantially retards the reduction of the pH-dependent Cl− efflux by DEPC. The findings that the apparent pK a is 6.5 in depolarized muscles, that DEPC eliminates the pH-dependent Cl− efflux, and that this action is retarded by SCN− supports the notion that protonation of histidine groups associated with Cl− channels is the controlling reaction for the pH-dependent Cl− efflux.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 55
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 69 (1982), S. 23-34 
    ISSN: 1432-1424
    Keywords: axon ; hydrostatic pressure ; Na currents ; kinetics ; temperature ; activation volume
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The effects of hydrostatic pressures up to 62 MPa upon the voltage-clamp currents of intact squid giant axons were measured using mineral oil as the pressure transmitting medium. The membrane resistance and capacitance were not appreciably affected over the whole range of pressures explored. The predominant effect of pressure is to slow the overall kinetics of the voltage-clamp currents. Both the early (Na) currents and the delayed (K) ones were slowed down by approximately the same time scale factor, which was in the range of 2 to 3 when pressure was increased from atmospheric to 62 MPa. Finer details of the effects, most evident at moderate depolarizations, are: the apparent initial delay in the turn-on of Na currents is increased by pressureless than is the phase of steepest time variation, and the later decay is slowedmore than is the rising phase. The initial time course of the currents at high pressures can be made to overlap with that at normal pressure by a constant time compression factor, Θm, together with a small, voltage-dependent delay. In a given axon, Θm was fairly independent of voltage, and it increased exponentially with pressure according to an apparent activation volume, ΔV∓, ranging between 32 and 40 cm3/mole. ΔV∓ tended to decrease with increasing temperature. Contrary to what is observed for moderate or large depolarizations, the kinetics of Na inactivation produced by conditioning prepulses of −50 or −60 mV was little affected over the whole range of pressures explored. Inferences about the pressure dependence of the steady-state Na activation were made from the comparison of the plots of early peak currents,I p, versus membrane potential,E. The Na reversal potential,E Na, and the slope of the plots nearE Na did not change significantly with pressure, but the peak Na conductancevs. E relationship was shifted by about +9 mV upon increasing pressure to 62 MPa. Steady-state Na inactivation,h ∞, was slightly affected by pressure. At 62 MPa the midpoint potential of theh ∞ (E) curve,E h, was shifted negatively by about 4 mV, while the slope atE h decreased by about 38%. Under the tentative assumption that pressure directly affects the gating of Na channels, the Na activation data follows a simple Hodgkin-Huxley scheme if the opening of anm gate involves an activation volume of about 58 Å3 and a net volume increase of about 26 Å3. However, a self-consistent description of the totality of the effects of pressure on Na inactivation cannot be obtained within a similar simple context.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 56
    ISSN: 1871-4528
    Keywords: gangrene ; climatic factors ; irrigation ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The incidence ofP. foveata in soil and tuber samples from field experiments often increased significantly as the harvest season progressed and temperatures fell. Mean levels of tuber and soil infestation were significantly higher in irrigated rows compared with those artificially sheltered from precipitation, both at normal and late harvest dates. Soil infestation was most frequently detected within 10 cm of the parent tuber. No pycnidia were visible on the haulm, and very few latently infected stem segments and leaves were detected using various methods. In three out of four years, the incidence ofP. foveata in tubers after wounding was reduced by washing them gently in tap water immediately after harvest.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 57
    ISSN: 1573-5176
    Keywords: Spirulina ; photobioreactor ; outdoor mass culture ; temperature ; night biomass loss ; carbohydrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Outdoor experiments carried out in Florence, Italy (latitude 43.8° N, longitude 11.3° E), using tubular photobioreactors have shown that in summer the average net productivity of a Spirulina platensis culture grown at the optimal temperature of 35 °C was superior by 23% to that observed in a culture grown at 25 °C. The rates of night biomass loss were higher in the culture grown at 25 °C (average 7.6% of total dry weight) than in the one grown at 35 °C (average 5%). Night biomass loss depended on the temperature and light irradiance at which the cultures were grown, since these factors influenced the biomass composition. A net increase in carbohydrate synthesis occurred when the culture was grown at a low biomass concentration under high light irradiance or at the suboptimal temperature of 25 °C. Excess carbohydrate synthesized during the day was only partially utilized for night protein synthesis.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 58
    ISSN: 1573-5176
    Keywords: Haematococcaceae ; palmella ; aplanospore ; acetate ; temperature ; nitrogen ; phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The freshwater green algaHaematococcus pluvialis (Strain Vischer 1923/2) grows best at high nitrate concentrations (about 0.5 to 1.0 g 1−1 KNO3), intermediate phosphate concentration (about 0.1 g 1−1 K2HPO4) and over a wide range of Fe concentrations. Low nitrate or high phosphate induce the formation of reddish palmella cells and aplanospores. Mixotrophic growth with acetate improves growth rate and final cell yield, and also stimulates the formation of the astaxanthin-containing palmella cells and aplanospores.H. pluvialis cannot grow above about 28 °C, or above a salinity of approximately 1% w/v NaCl. An increase in temperature or the addition of NaCl also stimulates the formation of palmella cells and aplanospores.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 59
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied phycology 3 (1991), S. 319-327 
    ISSN: 1573-5176
    Keywords: Dunaliella viridis ; growth ; salinity ; temperature ; nitrogen concentration ; batch culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of a strain ofD. viridis has been studied in batch culture under different combinations of temperature, salinity and nitrogen concentrations. Changes in these variables have a significant effect on cell division, biomass production, cell volume and pigment yield. This strain grows optimally at 1 M NaCl and 30 °C. Increasing salinity up to 4 M NaCl leads to a significant decrease of cell division rate and maximal population; growth at lower temperature decreases the rate of division of the cells but increases maximal cell density. Pigment yield decreases with increasing salinity and increases with increasing temperature. Nitrogen concentration has a large effect on total cell biomass and pigment production, but not on cell division rate. Saturation of growth occurs at 5 mM NO 3 − ; higher concentration (e.g. 10 mM) leads to a decrease of maximal cell density and photosynthetic pigment content.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 60
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied phycology 3 (1991), S. 319-327 
    ISSN: 1573-5176
    Keywords: Dunaliella viridis ; growth ; salinity ; temperature ; nitrogen concentration ; batch culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of a strain ofD. viridis has been studied in batch culture under different combinations of temperature, salinity and nitrogen concentrations. Changes in these variables have a significant effect on cell division, biomass production, cell volume and pigment yield. This strain grows optimally at 1 M NaCl and 30 °C. Increasing salinity up to 4 M NaCl leads to a significant decrease of cell division rate and maximal population; growth at lower temperature decreases the rate of division of the cells but increases maximal cell density. Pigment yield decreases with increasing salinity and increases with increasing temperature. Nitrogen concentration has a large effect on total cell biomass and pigment production, but not on cell division rate. Saturation of growth occurs at 5 mM NO 3 − ; higher concentration (e.g. 10 mM) leads to a decrease of maximal cell density and photosynthetic pigment content.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 61
    ISSN: 1573-5176
    Keywords: Haematococcaceae ; palmella ; aplanospore ; acetate ; temperature ; nitrogen ; phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The freshwater green algaHaematococcus pluvialis (Strain Vischer 1923/2) grows best at high nitrate concentrations (about 0.5 to 1.0 g 1−1 KNO3), intermediate phosphate concentration (about 0.1 g 1−1 K2HPO4) and over a wide range of Fe concentrations. Low nitrate or high phosphate induce the formation of reddish palmella cells and aplanospores. Mixotrophic growth with acetate improves growth rate and final cell yield, and also stimulates the formation of the astaxanthin-containing palmella cells and aplanospores.H. pluvialis cannot grow above about 28 °C, or above a salinity of approximately 1% w/v NaCl. An increase in temperature or the addition of NaCl also stimulates the formation of palmella cells and aplanospores.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 62
    ISSN: 1573-5125
    Keywords: Ferrissia wautieri ; fresh water limpet ; distribution in the Netherlands ; population dynamics ; population structure ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ferrissia wautieri, a freshwater limpet, is a widely distributed species in The Netherlands. In a pond near Nijmegen samples were taken twice a month over the year to study the population size and structure of this species in relation to the water temperature. Only ancyloids were found. Production of juveniles is temperature-dependent; peak numbers occurred in July and August. Just-hatched juveniles (shell length 0.6–1.0 mm) occurred over a lengthy period in the year, but were absent in March and April. During these months the collected numbers of specimens were very low. The largest specimens were collected during March, April and May.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 63
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 93 (1982), S. 191-193 
    ISSN: 1573-8221
    Keywords: human lymphocytes ; mitogenic lymphokines ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 64
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 94 (1982), S. 853-856 
    ISSN: 1573-8221
    Keywords: isolated myocardium ; contractility ; temperature ; stretching ; frequency of contractions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 65
    Electronic Resource
    Electronic Resource
    Springer
    Fish physiology and biochemistry 9 (1991), S. 179-187 
    ISSN: 1573-5168
    Keywords: temperature ; benzo(a)pyrene ; metabolism ; BAP-DNA adducts ; toadfish ; Opsanus beta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to examine the effects of temperature on benzo[a]pyrene (BaP) metabolism and adduct formation in the absence of the effects of temperature on uptake, gulf toadfish,Opsanus beta, were given a dose of 0.05 mg/kg3H-BaPvia caudal vein cannulae at their acclimation temperatures. (18 or 28°C) or following an acute temperature change (18 to 28°C or 28 to 18°C). After 72h, BaP-derived radioactivity was detected in all tissues examined and, as in otherin vivo studies of fish, the highest levels were found in the bile, the liver and the kidney. Temperature did not affect the total amount of BaP metabolized and excreted to the bile, but there were significant quantitative differences between temperature treatments in the classes of Phase I metabolites accumulated. Fish acclimated to high temperature accumulated more BaP triols and tetrols (breakdown products of highly carcinogenic BaP diol epoxides) than fish acclimated at low temperature regardless of exposure temperature: the proportion of biliary metabolites as tetrols and triols in each of the four temperature treatments (acclimation: exposure temperature), 28:18, 28:28, 18:18 and 18:28°C were 21.3±3.6, 58.1±6.1, 14.2±1.8 and 20.9±3.2% (mean±SEM, n=4), respectively. Significant quantities of BaP-DNA and BaP-hemoglobin adducts were detected; however, only the amounts of BaP-DNA adducts showed sensitivity to temperature. As predicted from our metabolite data, high acclimation or exposure temperature led to a significant increase in the amount of BaP-DNA adducts formed: adduct formation in the temperature treatments, 28:18, 28:28, 18:18 and 18:28°C were 342±52, 526±51, 155±42 and 252±55 fg BaP/ µg DNA (mean±SEM, n=4), respectively. These results are discussed in the context of mechanisms of high temperature-enhancement of carcinogenesis in fish.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 66
    Electronic Resource
    Electronic Resource
    Springer
    Fish physiology and biochemistry 9 (1991), S. 261-269 
    ISSN: 1573-5168
    Keywords: Cortisol ; RU 486 ; temperature ; rainbow trout ; cell culture ; [3H]-Thymidine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rainbow trout gonadal cell line, RTG-2, which survives temperatures from 0 to 28°C and proliferates at 5 to 26°C, responded to cortisol from 28°C to 0°C by influencing [3H]-thymidine incorporation into DNA. Over the normal temperature range of rainbow trout, 10–22°C, cortisol inhibited [3H]-thymidine incorporation. The antiglucocorticoid RU 486 had no effect on [3H]-thymidine incorporation at these temperatures and blocked the response to cortisol. Another antiglucocorticoid RU 362 also had no effect but was less effective in blocking the cortisol response. During incubation at 28°C this inhibitory response to cortisol was detected inconsistently during the first 24 h but was observed consistently during the second 24 h. At 0°C, cortisol and RU 486 had no effect during short treatments, but a 60 h exposure to either steroid stimulated [3H]-thymidine incorporation over a 48 h labelling period. These results suggest that temperature shifts between 10–22°C, do not change the direction of a response to cortisol and support the use of the upper portion (20–22°C) of the temperature range for studies on salmonid cells in culture.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 67
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plant pathology 88 (1982), S. 191-202 
    ISSN: 1573-8469
    Keywords: coffee leaf rust ; complete resistance ; major gene resistance ; temperature ; heterogeneous reaction type ; components of resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Resistentie van koffie tegen fysio II vanHemileia vastatrix werd getoetst in milieus bij lichtintensiteiten (LI), die varieerden van 17 tot 100% van de totale instraling. Negen behandelingen, bestaande uit de combinaties van drie niveaus van LI vóór inoculatie en drie ná inoculatie, werden toegepast op zaailingen van het vatbareCoffea arabica ras Mundo Novo. Toenemende LI vóór inoculatie veroorzaakte een significante toename in lesiedichtheid, terwijl het tegenovergestelde werd waargenomen bij de behandeling na inoculatie. Maximale verschillen in lesiedichtheid waren drievoudig. De interactie tussen behandelingen vóór en ná inoculatie was ook significant. Bij extreem hoge LI ná inocultie trad necrose van de lesies op. Genotypen van de Icatu populatie en van hetC. canephora ras Kouillou, met verschillende ziektescores in het veld, werden beproefd in verschillende milieus, waarbij een constante LI voor en na inoculatie werd toegepast. De resistentie van de meeste genotypen kwam beter tot uiting bij lage LI dan bij hoge LI, wat ook waargenomen werd voor het controle ras Mundo Novo. Bij het ras Kouillou werden de dichtheid van sporulerende lesies, de latentieperiode en het reactietype significant beïnvloed door LI en genotype. De interactie tussen LI en genotype was ook significant voor dichtheid van sporulerende lesies en voor reactietype, voornamelijk doordat het meest resistente genotype niet, of in de omgekeerde richting, beïnvloed werd door LI. De expressive van het resistentiegen Sh4 bleek ook afhankelijk van het milieu. Waarnemingen aan een uitsplitsende F2-populatie duidden op een dominante genwerking in de kas (lage LI) en een incompleet dominante, of bijna recessieve, genewerking in de kwekerij (hoge LI). Deze incomplete dominantie uitte zich d.m.v. heterogene tot vatbare reactietypes van heterozygote planten (SH4sH4) onder hoge LI. Enkele ecologische en veredelingstechnische aspecten van de waargenomen invloed van LI worden besproken.
    Notes: Abstract Resistance of coffee to race II ofHemileia vastatrix was tested in different environments at light intensities (LI) from 17 to 100% of total outdoor radiation. Nine treatments, in which three levels of LI before inoculation were combined with three levels of LI after inoculation, were applied to seedlings of the susceptible cv. Mundo Novo. Higher LI before inoculation induced a significant increase in lesion density, whereas the opposite was observed for treatments after inoculation. Maximum differences in lesion density were threefold. The interaction between pre-and post-inoculation treatments was also significant. Necrosis of lesions occurred under extremely high LI after inoculation. Genotypes of the Icatu population and ofCoffea canephora cv. Kouillou, which varied in disease level in the field, were tested in different environments, constant LI being applied before and after inoculation. Most genotypes were more resistant at low LI than at high LI, paralleling the results obtained for the control cv. Mundo Novo. With cv. Kouillou, sporulating lesion density, latency period and reaction type were significantly affected by LI and genotype. The interaction between LI and genotypes was significant for sporulating lesion density and reaction type, mainly because the most resistent genotype was not affected, or affected in opposite direction, by LI. Environment affected the expression of the resistance gene SH4. Observations on a segregating F2 population indicated dominant gene action in the greenhouse (low LI) and incomplete dominant to nearly recessive gene action in the nursery (high LI). Incomplete dominance was expressed by heterogeneous to susceptible reaction types of heterozygote plants (SH4sH4), under high LI. Some ecological and breeding aspects of the observed effect of LI on resistance to coffee leaf rust are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 68
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 230 (1991), S. 241-250 
    ISSN: 1617-4623
    Keywords: Yeast ; Saccharomyces cerevisiae ; Adenylyl cyclase ; CDC25 ; RAS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The TFS1 gene of Saccharomyces cerevisiae is a dosage-dependent suppressor of cdc25 mutations. Overexpression of TFS1 does not alleviate defects of temperature-sensitive adenylyl cyclase (cdc35) or ras2 disruption mutations. The ability of TFS1 to suppress cdc25 is allele specific: the temperature-sensitive cdc25-1 mutation is suppressed efficiently but the cdc25-5 mutation and two disruption mutations are only partially suppressed. TFS1 maps to a previously undefined locus on chromosome XII between RDN1 and CDC42. The DNA sequence of TFS1 contains a single long open reading frame encoding a 219 amino acid polypeptide that is similar in sequence to two mammalian brain proteins. Insertion and deletion mutations in TFS1 are haploviable, indicating that TFS1 is not essential for growth.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 69
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 226 (1991), S. 97-106 
    ISSN: 1617-4623
    Keywords: Yeast ; Linear plasmid ; Saccharomyces kluyveri ; Kluyveromyces lactis ; Killer plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have determined the complete nucleotide sequence of the linear DNA plasmid, pSKL, isolated from Saccharomyces kluyveri. Sequence analysis showed that pSKL has a high (A+T) content of 71.7%, and that there are 10 open reading frames (ORFs) larger than 250 nucleotides. All 10 ORFs were shown to be transcribed in S. kluyveri cells by S1 nuclease mapping analysis. The localization of ORFs, direction of transcription, and the predicted amino acid sequences of each ORF were quite similar to that of pGKL2, one of the killer plasmids found in Kluyveromyces lactis. The amino acid sequences of the largest two ORFs (ORF2 and ORF6) have homology with several DNA polymerases and RNA polymerases, respectively.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 70
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 226 (1991), S. 145-153 
    ISSN: 1617-4623
    Keywords: Retrotransposons ; Reverse transcription ; Ty elements ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Transposition of the yeast transposable element, Ty, has been shown to require a reverse transcription process. By analysing the extrachromosomal Tyspecific nucleic acid molecules associated with overproduced Ty virus-like particles (Ty-VLPs), we identified several reverse transcribed cDNA strands. Most of them resemble the characteristic intermediates of the reverse transcription process described for authentic retroviruses: a (−) strong-stop DNA strand covalently bound to an RNA primer, two elongated (−) strands with one or two long terminal repeat (LTR) sequences and a (+) strong-stop DNA. Surprisingly, complete (+) strands and full-length linear duplex Ty DNA could not be detected. The structural features of two additional (÷) strands may indicate some differences between the mechanisms of (+) strand synthesis in Ty and other retrotransposons or retroviruses.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 71
    ISSN: 1617-4623
    Keywords: Yeast ; Arginine ; Sequence ; Regulation ; Control region
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Saccharomyces cerevisiae, the ARG5,6 gene encodes acetylglutamyl-P reductase and acetylglutamate kinase, two arginine anabolic enzymes which are localized in the mitochondria. The synthesis of both enzymes is co-ordinately controlled by arginine and by three regulatory proteins (ARGRI, ARGRII, and ARGRIII). The ARG5,6 gene was cloned by complementation of an arg5 mutant strain. A subclone containing an EcoRI fragment of about 3.2 kb which complements the arginine requirement was sequenced. This 3163 by sequence contains only one long open reading frame of 2589 nucleotides encoding a protein of 863 amino acids. The size of this protein is in agreement with the length of the unique transcript determined by Northern hybridization. The measurements of ARG5,6 mRNA under various regulatory conditions show no correlation with the enzyme levels. As in other arginine biosynthetic and catabolic genes, the regulation by arginine through the three ARGR proteins thus involves a post-transcriptional control mechanism. By in vitro mutagenesis we created point mutations and deletions in the 5′ non-coding region of the ARG5,6 gene which allowed us to define the primary target of ARGR control. Specific regulation involves two regions: one located between the putative TATA element and the transcriptional initiation site and the second between this site and the first ATG.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 72
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 229 (1991), S. 413-420 
    ISSN: 1617-4623
    Keywords: SCO1 ; Cytochrome oxidase ; Membrane protein ; Mitochondria ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The SCO1 gene of Saccharomyces cerevisiae encodes a 30 kDa protein which is specifically required for a post-translational step in the accumulation of subunits 1 and 2 of cytochrome c oxidase (COXI and COXII). Antibodies directed against a β-Gal::SCO1 fusion protein detect SCO1 in the mitochondrial fraction of yeast cells. The SCO1 protein is an integral membrane protein as shown by its resistance to alkaline extraction and by its solubilization properties upon treatment with detergents. Based on the results obtained by isopycnic sucrose gradient centrifugation and by digitonin treatment of mitochondria, SCO1 is a component of the inner mitochondrial membrane. Membrane localization is mediated by a stretch of 17 hydrophobic amino acids in the amino-terminal region of the protein. A truncated SCO1 derivative lacking this segment, is no longer bound to the membrane and simultaneously loses its biological function. The observation that membrane localization of SCO1 is affected in mitochondria of a rho 0 strain, hints at the possible involvement of mitochondrially coded components in ensuring proper membrane insertion.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 73
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 225 (1991), S. 363-368 
    ISSN: 1617-4623
    Keywords: Yeast ; Metallothionein gene ; Cadmium resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 3.3 kb fragment of yeast genomic DNA was isolated by screening a genomic library constructed in the high copy number 2 micron plasmid YEp351 vector for clones capable of enhancing the degree of resistance of Saccharomyces cerevisiae strain MW3070-8B to cadmium. The insert contained two complete copies of the CUP1 gene open reading frame (183 bp), including the upstream promoter sequences (450 bp) with two conserved metal responsive cis-acting elements. Northern analysis showed that addition of cadmium (0.02 μM) or copper (50 μM) to overnight liquid cultures of yeast induced expression of CUP1 transcripts from both chromosomal and plasmid-borne gene copies. The cloned 3.3 kb DNA in a high copy number plasmid restored copper resistance to the sensitive strain LS70-313Δ, deleted for the CUP1 gene (cup1Δ), but failed to restore cadmium resistance. Thus, CUP1 gene expression in yeast appears to be influenced differently by cadmium and copper ions. Resistance to heavy metal poisoning resulted from enhanced gene product levels attributable to amplification of the CUP1 gene as well as to increased transcriptions. Two distinct gene product levels mediate cadmium and copper resistance; a higher gene product level was required to confer cadmium resistance.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 74
    ISSN: 1617-4623
    Keywords: Meiosis ; Sporulation ; Northern hybridization ; Regulatory circuit ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The SGA1 gene encoding glucoamylase is specifically expressed late in meiotic development of the yeast Saccharomyces cerevisiae. We found that accumulation of both enzyme activity and transcripts was regulated negatively by both nutritional signals and a haploid-specific negative regulator gene of meiosis, RME1, and positively by the inducer genes for meiosis, IME1 and IME2. To study the role of sequences upstream of the SGA1 gene in its expression and regulation, we generated internal deletions in the 5′ non-coding region of the gene and chimeric genes with portions of the upstream sequence inserted into a reporter gene. By analyzing the expression of these genes, we have identified both a 19 by upstream activation sequence (UAS) and a 49 by negatively regulating element (NRE). The UAS activated transcription with no requirement for heterozygosity at the mating-type locus, but this activation was still under negative control by nutrients. The NRE showed no UAS-like activity but conferred IME2-dependent (or meiosis-specific) expression on a heterologous promoter. These results suggest that meiosis-specific expression of the SGA1 gene is established by a regulatory hierarchy including positive and negative factors, the actions of which are mediated through the two separate upstream regulatory elements, UAS and NRE, respectively. Also, that two independently acting cascades exist for the regulation of SGA1 expression: one transduces both the mating-type and nutritional signals and includes the IME2 product, which acts to relieve the repression through NRE ; and another transduces only the nutritional signal independently of the above pathway and inhibits positive factors acting on UAS.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 75
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 227 (1991), S. 127-136 
    ISSN: 1617-4623
    Keywords: Yeast ; Killer toxin ; Immunity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cDNA copy of the M2 dsRNA encoding the K2 killer toxin ofSaccharomyces cerevisiae was expressed in yeast using the yeastADH1 promoter. This construct produced K2-specific killing and immunity functions. Efficient K2-specific killing was dependent on the action of the KEX2 endopeptidase and the KEX1 carboxypeptidase, while K2-specific immunity was independent of these proteases. Comparison of the K2 toxin sequence with that of the K1 toxin sequence shows that although they share a common processing pathway and are both encoded by cytoplasmic dsRNAs of similar basic structure, the two toxins are very different at the primary sequence level. Site-specific mutagenesis of the cDNA gene establishes that one of the two potential KEX2 cleavage sites is critical for toxin action but not for immunity. Immunity was reduced by an insertion of two amino acids in the hydrophobic amino-terminal region which left toxin activity intact, indicating an independence of toxin action and immunity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 76
    ISSN: 1617-4623
    Keywords: Yeast ; Mitochondria ; Frameshift ; Suppression ; Restriction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The +1 frameshift mutation, M5631, which is located in the gene (oxi1) for cytochrome c oxidase II (COXII) of the yeast mitochondrial genome, is suppressed spontaneously to a remarkably high extent (20%–30%). The full-length wild-type COXII produced as a result of suppression allows the mutant strain to grow with a “leaky” phenotype on non-fermentable medium. In order to elucidate the factors and interactions involved in this translational suppression, the strain with the frameshift mutation was mutated by MnCl2 treatment and a large number of mutants showing restriction of the suppression were isolated. Of 20 mutants exhibiting a strong, restricted, respiration-deficient (RD) phenotype, 6 were identified as having mutations in the mitochondrial genome. Furthermore, genetic analyses mapped one mutation to the vicinity of the gene for tRNAPro and two others to a region of the tRNA cluster where two-thirds of all mitochondrial tRNA genes are encoded. The degree of restriction of the spontaneous frameshift suppression was characterized at the translational level by in vivo 35S-labeling of the mitochondrial translational products and immunoblotting. These results showed that in some of these mutant strains the frameshift suppression product is synthesized to the same extent as in the leaky parent strain. It is suggested that more than one +1 frame-shifted product is made as a result of suppression in these strains: one is as functional as the wild-type COXII, the other(s) is (are) non-functional and prevent leaky growth on non-fermentable medium. A possible mechanism for this heterogenous frameshift suppression is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 77
    ISSN: 1617-4623
    Keywords: SNF2 sequence ; Transcriptional regulator ; Gene expression ; Glucoamylase ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have cloned and sequenced the GAM1 gene which is required for transcription of the STA1 gene encoding an extracellular glucoamylase in Saccharomyces cerevisiae var. diastaticus. Complementation tests indicated that GAM1 is the same gene as SNF2 which is required for derepression of the SUC2 gene encoding invertase. Accumulation of SNF2 RNA was not regulated by the GAM2 and GAM3 genes which are also required for STA1 expression. The SNF2 gene was predicted to encode a 194 kDa highly charged protein with a glutamine-rich tract. A bifunctional SNF2-lacZ fusion protein was shown by immunofluorescence microscopy to be localized to the nucleus, suggesting that the SNF2 protein is located in the nucleus.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 78
    ISSN: 1617-4623
    Keywords: Protein kinase ; Yeast ; CDC28 ; Cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A novel protein kinase homologue (KNS1) has been identified in Saccharomyces cerevisiae. KNS1 contains an open reading frame of 720 codons. The carboxy-terminal portion of the predicted protein sequence is similar to that of many other protein kinases, exhibiting 36% identity to the cdc2 gene product of Schizosaccharomyces pombe and 34% identity to the CDC28 gene product of S. cerevisiae. Deletion mutations were constructed in the KNS1 gene. kns1 mutants grow at the same rate as wild-type cells using several different carbon sources. They mate at normal efficiencies, and they sporulate successfully. No defects were found in entry into or exit from stationary phase. Thus, the KNS1 gene is not essential for cell growth and a variety of other cellular processes in yeast.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 79
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 229 (1991), S. 353-356 
    ISSN: 1617-4623
    Keywords: DNA polymerase ; Gene conversion ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Saccharomyces cerevisiae, three different DNA polymerase complexes, POLI, POLII and POLIII, are known to be involved in DNA replication. The catalytic subunit of POLIII is encoded by the essential CDC2 gene. The existence of different thermosensitive non-complementing mutants of CDC2 offers the possibility of using a genetic approach to investigate the involvement of POLIII in induced gene conversion. When cdc2 heteroallelic cells were irradiated and incubated under restrictive conditions, almost no induction of thermoresistant cells could be detected, suggesting an essential role for POLIII in mitotic gene conversion events.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 80
    ISSN: 1617-4623
    Keywords: Translational activation ; Cytochrome b ; Mitochondria ; Yeast ; CRS1 ; CBS2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The products of the nuclear genes CBS1 and CBS2 are both required for translational activation of mitochondrial apocytochrome b in yeast. We report the intramitochondrial localization of both proteins by use of specific antisera. Based on its solubilization properties the CBS1 protein is presumed to be a component of the mitochondrial membrane; the detergent concentrations needed to release CBS1 from mitochondria are almost the same as for cytochrome c 1. In contrast, CBS2 behaves like a soluble protein, with some characteristics of a membrane-associated protein. A model is presented for translational activation of cytochrome b, which might also be applicable to translational regulation of other mitochondrial genes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 81
    ISSN: 1617-4623
    Keywords: Yeast ; Transcription ; a- and α-specific genes ; MCM1 ; STE12
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have examined the relative contributions of MCM1 and STE12 to the transcription of the a-specific STE2 gene by using a 367 by fragment from the STE2 5′-noncoding region to drive expression of a reporter lacZ gene. Mutation of the MCM1 binding site destroyed MCM1 · α2-mediated repression in α cells and dramatically reduced expression in a cells. The residual expression was highly stimulated by exposure of cells to pheromone. Likewise, the loss of STE12 function reduced lacZ expression driven by the wild-type STE2 fragment. In the absence of both MCM1 and STE12 functions, no residual expression was observed. Thus, the STE2 fragment appears to contain two distinct upstream activation sequences (UASs), one that is responsible for the majority of expression in cells not stimulated by pheromone, and one that is responsible for increased expression upon pheromone stimulation. In further support of this idea, a chemically synthesized version of the STE2MCM1 binding site had UAS activity, but the activity was neither stimulated by pheromone nor reduced in ste12 mutants. Although transcription of aspecific genes also requires both MCM1 and STE12, these genes differ from a-specific genes in that they have a single, MCM1-dependent UAS system. The activity of the minimal 26 by UAS from the α-specific STE3 gene was both stimulated by pheromone and reduced in ste12 mutants. These data suggest that at α-specific genes STE12 and MCM1 exert their effects through a single UAS.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 82
    ISSN: 1617-4623
    Keywords: Mitochondria ; Yeast ; Protein targeting ; PET2858 ; Inner membrane protease 1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nuclear yeast mutant pet ts2858 is defective in the removal of pre-sequences from the mitochondrially encoded cytochrome oxidase subunit II (COXII) and the processing intermediate of cytochrome b 2 (Cytb 2), a nuclear gene product. In order to identify the genetic lesion in this mutant we have cloned and characterized a DNA region which complements the pet ts2858 mutation. The DNA sequence revealed three open reading frames, one of which is responsible for the complementation. A 570 by reading frame represents the structural gene PET2858, as demonstrated by in vitro mutagenesis, gene expression from a foreign promoter, and allelism tests. PET2858 encodes a 21.4 kDa protein, which is essential for growth on non-fermentable carbon sources and for the proteolytic processing of COXII and the Cytb 2 intermediate. When the N-terminus of the PET2858 protein is fused to a reporter protein, the resulting hybrid molecule is imported into mitochondria. Interestingly, the N-terminal half of the deduced PET2858 protein exhibits 30.7% amino acid identity to the leader peptidase of Escherichia coli. These results suggest that PET2858 codes for a mitochondrial inner membrane protease (IMP1) or at least a subunit of it. This protease is involved in protein processing and export from the mitochondrial matrix.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 83
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 133 (1991), S. 47-56 
    ISSN: 1573-5036
    Keywords: clover ; competition ; grass ; Lolium perenne ; 15N ; nitrogen fixation ; Phleum pratense ; temperature ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract It was the aim of this study to determine the way in which low temperature modifies the effect of a competing grass on nitrogen fixation of a forage legume. White clover (Trifolium repens L.) was grown in monoculture or in different planting ratios with timothy (Phleum pratense L.) or perennial ryegress (Lolium perenne L.) in growth chambers at either 7.5/5°C (LoT) or 15/10°C (HiT) average day/night temperatures, and with 2.5 or 7.5 mM 15N-labelled nitrate in the nutrient solution. Competition with grass led to a marked increase in the proportion of clover nitrogen derived from symbiosis (% Nsym). This increase was slower at LoT where % Nsym was reduced considerably; it was closely related to the reduction in the amount of available nitrate as a result of its being utilized by the grass. Nitrogen concentration in white clover herbage and dry matter yield per clover plant were reduced, for the most part, when a competing grass was present. The amount of nitrogen fixed per plant of white clover decreased markedly with temperature. Low temperature consequently accentuated competition for nitrate. The capacity of white clover to compete successfully was limited by its slower growth and nitrogen accumulation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 84
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 31 (1982), S. 485-492 
    ISSN: 1573-5060
    Keywords: Chrysanthemum morifolium ; light ; temperature ; selection ; genotype environment interaction ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Chysanthemum cultivars were grown in 4 experiments in the greenhouse and in the phytotron. The growing conditions in the greenhouse varied from 14 C, 60 J cm2 day-1 to 17 C. 431 J cm2 day-1, those in the phytotron from 12 to 455 J cm-2 day-1 at a temperature of either 14 or 20 C. Most cultivars responded differentially to light and/or temperature, but this was only measurable when large differences in light and/or temperature were imposed. Reducing the light level in a greenhouse by 50% does not bring out cultivar×light interactions. Such interactions may be pronounced however when the performance in summer is compared with that in winter. This is also reflected in the correlation coefficients. The correlations between summer and winter performance for number of flowers per stem and the number of days to flowering are non-significant, those for the 7 other traits measured were all significant. The occurrence of interactions does not preclude the possibility of breeding cultivars adapted to a wide range of light and temperature conditions. Appropriate selection procedures are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 85
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 136 (1991), S. 31-39 
    ISSN: 1573-5036
    Keywords: biocontrol ; carrots ; flooding ; fungicides ; Phytophthora megasperma ; Pythium acanthicum ; Rhizoctonia solani ; seedling diseases ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In naturally infested soil containingPythium ultimum, P. acanthicum andPhytophthora megasperma, onlyP. ultimum was associated with root rot and damped-off seedlings. Damping-off was promoted by low soil temperatures and by flooding. Seedling stands were markedly reduced when seed was pre-incubated in soil at 12°C but not at 25°C or 35°C. Dusting carrot seed with metalaxyl significantly increased seedling stands in the field at rates from 1.5–6 g kg−1 seed and in both flooded and unflooded, naturally infested soil at 3.15 g kg−1. In greenhouse experiments using artifically infested soil,P. ultimum andP. paroecandrum caused damping-off of carrot seedlings andRhizoctonia solani reduced root and shoot weights.R. solani caused damping-off in nutrient-enriched soil.P. acanthicum andP. megasperma were not pathogenic to seedlings, although both fungi colonized roots. Soil populations of allPythium spp., particularlyP. ultimum, increased during growth of seedlings and population growth ofP. megasperma was promoted by periodic flooding. Infestation of soil withP. acanthicum did not reduce damping-off of carrot seedlings byP. ultimum orP. paroecandrum, but significantly increased root and shoot weights and decreased root colonization byR. solani P. acanthicum has potential as a biocontrol agent againstR. solani.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 86
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 137 (1991), S. 191-199 
    ISSN: 1573-5036
    Keywords: durixerollic calciorthid soil ; evapotranspiration ; plant water relations ; silicon transport soil ; temperature ; Triticum aestivum ; uptake ; water balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Silicon (Si) content in cereal plants and soil-Si solubility may be used to estimate transpiration, assuming passive Si uptake. The hypothesis for passive-Si uptake by the transpiration stream was tested in wheat (Triticum aestivum cv. Stephens) grown on the irrigated Portneuf silt loam soil (Durixerollic calciorthid) near Twin Falls, Idaho. Treatments consisted of 5 levels of plant-available soil water ranging from 244 to 776 mm provided primarily by a line-source sprinkler irrigation system. Evapotranspiration was determined by the water-balance method and water uptake was calculated from evapotranspiration, shading, and duration of wet-surface soil. Water extraction occurred from the 0 to 150-cm zone in which equilibrium Si solubility (20°C) was 15 mg Si L−1 in the Ap and Bk (0–58 cm depth) and 23 mg Si L−1 in the Bkq (58–165 cm depth). At plant maturity, total Si uptake ranged from 10 to 32 g m−2, above-ground dry matter from 1200 to 2100 g m−2 and transpiration from 227 to 546 kg m−2. Silicon uptake was correlated with transpiration (Siup=−07+06T, r2=0.85) and dry matter yield with evapotranspiration (Y=119+303ET, r2=0.96). Actual Si uptake was 2.4 to 4.7 times that accounted for by passive uptake, supporting designation of wheat as a Si accumulator. The ratio of Si uptake to water uptake increased with soil moisture. The confirmation of active Si uptake precludes using Si uptake to estimate water use by wheat.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 87
    ISSN: 1573-5117
    Keywords: Cladocera ; clones ; Daphniidae ; gamogenesis ; parthenogenesis ; Pennsylvania ; photoperiod ; temperature ; Scapholeberis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The induction of gamogenesis in a dicyclic population of Scapholeberis armata from a small pond at 40° N in Pennsylvania, USA, was studied. Animals were collected at the time of gamogenesis in mid-October (fall) 1988 and early June (spring) 1989). 10 clones were established from isolated females for each season and maintained at low density under standard conditions of 20 °C and 13.5L/10.5D, with Chlamydomonas reinhardtii diluted with pond water to 3 · 105 cells ml−1 as a food source. For each clone, mothers of pairs of test animals were acclimated to 4 sets of conditions: 20 °C/15.5L (spring), 15 °C/11.5L (fall), 20 °C/11.5L (control), and 15 °C/15.5L. Test animals were observed bidaily or daily for up to 37 days. Occurrence of molts and of parthenogenetic broods or ephippia was recorded. At short photoperiod, ephippial females developed, usually after one or more parthenogenetic broods, among both spring and fall animals. The response was stronger (higher % sexual females) among fall clones, and was enhanced in both groups by 15 °C water. No animals responded to long photoperiod in warm or cool water. It is likely that additional factors, such as prior photoinduction, rapid depletion of food supply, and increased inter-animal contact due to high population density are required for the induction of spring gamogenesis. Differences in the responsiveness of spring and fall clones to inducing conditions suggest some differences in genotypes of spring and fall subpopulations in this pond. It is proposed that variable responses among genotypes to factors inducing gamogenesis may be the basis for the differences and clines in the timing of gamogenesis found among ecologically and latitudinally separated populations.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 88
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 218 (1991), S. 151-156 
    ISSN: 1573-5117
    Keywords: aquatic hyphomycetes ; sulphur spring ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aquatic hyphomycetes of a sulphur spring in the Western Ghat region of Karnataka were investigated by the following methods: leaf litter observations, water filtration, analysis of natural and induced foam. The samples were collected and studied from three sites, the spring proper (site I), the connecting region of the spring and the rivulet (site II) and the spot where the spring joins the rivulet (site III). The number of species of hyphomycetes encountered at the three sites were 1, 14 and 20, respectively. The sulphide content of the spring ranged between 0.1–3.1 mg l−1 and the temperature between 26.5–36.5 °C. In another experiment submerged coffee leaves from a fresh water stream were collected and incubated in spring water, stream water and well water at different temperatures (16–40 °C). A maximum of 18 species of hyphomycetes sporulated at 22 °C followed by 17 species at 28 °C, irrespective of the source of water in which the leaves were incubated. Based on these studies, we conclude that the presence of only one species in the spring proper (site I) was not due to the higher sulphide content, but to the high temperature.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 89
    ISSN: 1573-5117
    Keywords: Biomphalaria ; Bulinus ; Helisoma ; schistosomiasis ; biological control ; temperature ; darkness ; starvation ; food
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Helisoma duryi has been proposed as a biological control agent in schistosomiasis due to its superiority in laboratory competition experiments with various species of the intermediate host snails. Therefore it was considered important to evaluate the response of this snail species and the intermediate host species, Biomphalaria alexandrina and Bulinus truncatus, to various physical, chemical and biological factors under laboratory conditions in order to obtain information on the similarities in the ecological niches of these species. The factors considered in the present paper are: temperature, darkness, starvation and food. All three species had optimal growth and egg laying at 26–28 °C. Only H. duryi survived for a longer period at 33°C and it was capable of starting egg laying at this temperature although the onset was delayed. However, low temperature (18°C) caused a relatively larger decrease in egg laying of H. duryi than in the other two species. Growth and egg laying was reduced for H. duryi and B. truncatus kept under darkness and B. alexandrina could not tolerate maintenance under darkness. A few days of starvation of juvenile snails had no effect on later growth and egg laying capacity of the survivors, although mortality in B. truncatus was increased. B. alexandrina had a lower tolerance to starvation than the other two species. Egg laying of snails fed only one of the three laboratory food types decreased for all three species in the order: Vov-vov (dog food in dry pellets), Tetramin (fish food) and lettuce. Combinations of lettuce and one or more proteinaceous food types gave optimal growth and egg laying for all three species.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 90
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 89 (1982), S. 177-188 
    ISSN: 1573-5117
    Keywords: Chironomidae ; littoral ; reservoir ; salinity ; chlorides ; particulate organic matter ; temperature ; wind
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lake Texoma in southcentral Oklahoma was formed by the impoundment of the Washita and Red Rivers. The Red River is more highly saline than the Washita and creates a complex salinity gradient across the reservoir. Populations of chironomids were monitored with multiple-plate samplers in areas of high (34–113 mg l−1 Cl−), intermediate (35–60 mg l−1 Cl−) and low (4–27 mg l−1 Cl−) salinity during the spring and summer of 1978. Food availability, temperature, salinity and wind direction influenced the distribution of the 14 genera and at least 22 species of chironomids which colonized the multiple-plate samplers. Filter-feeders attained their highest densities in the river-arm stations where levels of particulate organic matter (POM) were high. Algal grazers attained their highest densities in the clear intermediate area where the plates of the samplers were covered with algal mats. Most of the genera believed to be feeding primarily on POM decreased in density as the temperature and density of Glyptotendipes sp. rose. Certain species were restricted to either the Red River arm or the Washita River arm and this is probably a reflection of different salinity tolerances.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 91
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 91-92 (1982), S. 651-658 
    ISSN: 1573-5117
    Keywords: stream ; sediment ; sand ; hyporheal ; oxygen ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dissolved oxygen, pH, conductivity and alkalinity of surface and subsurface interstitial waters were investigated at Mill Creek (a small, rural, predominantly sandy stream in east Texas). Dissolved oxygen concentration tended to decrease with sediment depth, while conductivity and alkalinity did not significantly change with substrate depth. Surface water pH was significantly higher than interstitial water (p = 0.05). Chemical analyses of subsurface water from a pre- to post-storm event showed a depression in dissolved oxygen concentration in moderate and deep interstitial waters, immediately following the return of stream base flow, with the deeper strata returning to pre-storm oxygen levels four days later. A thermal convective current mechanism is proposed which would serve to transport surface water downward into these deeper interstices.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 92
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 93 (1982), S. 23-30 
    ISSN: 1573-5117
    Keywords: Mysidacea ; salinity tolerance ; temperature ; Mesopodopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Separate and combined effects of changes in salinity and water temperature on the survival of laboratory hatched juvenileMesopodsis orientalis were investifated. Full strength seawater (35‰) was not favorable to juvenile survival. Salinities down to 10% seawater were tolerated when subjected to sudden exposure, but salinity acclimation increased juvenile ability to tolerate even fresh water. Water temperatures tolerated by the animals ranged from 12°C to 33°C. Salinities of 30% to 60% seawater and water temperatures of 22°C to 28°C were most favorable to the juveniles. Experimental results were compared with field observations and a relationship between the salinity and temperature of seawater and abundance of juveniles in May to June, and November to January in Bombay coastal waters (west coast of India) was established. Seawater of reduced salinity was found to be a major factor for occurrence of juvenileM. orientalis in abundance.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 93
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 26 (1991), S. 167-171 
    ISSN: 1573-5044
    Keywords: adventitious buds ; Aloe barbadensis ; in vitro propagation ; sucrose ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Axillary bud development and adventitious bud formation was obtained with decapitated shoot explants of Aloe barbadensis Mill. Maximal bud growth and rooting of shoots was obtained on a modified medium of Murashige and Skoog supplemented with 5 μM IBA. More adventitious and axillary buds developed on nutrient media supplemented with IBA than with NAA. Axillary buds but not adventitious buds developed with IAA in the medium. Morphogenesis was inhibited by 2,4-D. Kinetin, benzyladenine and thidiazuron were toxic to the explants and did not stimulate the development of axillary of adventitious buds. The optimal temperature for bud growth and development was 25°C. Axillary bud growth and the formation of adventitious buds was slowed down at 10°C and totally inhibited by 30°C. The optimal sucrose concentration was 3% with the inhibition of bud growth and development by higher sucrose levels.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 94
    Electronic Resource
    Electronic Resource
    Springer
    Antonie van Leeuwenhoek 59 (1991), S. 125-127 
    ISSN: 1572-9699
    Keywords: Taxonomy ; Williopsis Salicorniae ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four strains of an undescribed species of the genus Williopsis were isolated from brackish water. A description of the new species, Williopsis salicorniae (type strain, CBS 8071, NRRL Y-12834), is given.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 95
    Electronic Resource
    Electronic Resource
    Springer
    Journal of atmospheric chemistry 12 (1991), S. 381-390 
    ISSN: 1573-0662
    Keywords: Ozone ; total ozone ; temperature ; stratosphere ; stratospheric warming ; Antarctic ozone hole ; polar stratospheric clouds ; ozone depletion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract On 1 February 1989, -83.5°C was recorded in 27.8 hPa over Hohenpeißenberg, the lowest temperature in the 22-year series. This was measured together with a very low total ozone amount of 266 DU. This may be compared with nearly twice this amount on 27 February 1989. The situation was very unusual: following an extremely cold winter in the Arctic stratosphere, the stratospheric cold pole was located over southern Scandinavia on 1 February in a very southerly position. The analyzed temperatures of -92 °C in 30 hPa were also unusual. Even though the low ozone amounts over Hohenpeißenberg were probably dynamically caused, an additional very small ozone decrease due to heterogeneous reactions in altitudes from 23–28 km, where the temperatures lie below -80 °C, cannot be ruled out. Extinction measurements by the orbitting SAGE II instrument indeed show polar stratospheric clouds over Europe near 50° N during the period 31 January–2 February. Also, polar stratospheric clouds were previously observed over Kiruna at similarly low temperatures and signs of a corresponding small ozone decrease were noted there.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 96
    ISSN: 1573-5117
    Keywords: freshwater snail ; Indoplanorbis ; reproduction ; schistosomiasis ; temperature ; vector
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The direct and delayed effect of short-term exposure to low temperature (17.5 °C) on growth, reproduction and longevity of adolescent and mature freshwater Indoplanorbis exustus Deshyaes (Mollusca: Gastropoda) was studied in the laboratory. We found that growth and reproduction were retarded, but were enhanced when later they were returned to ambient temperature.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 97
    ISSN: 1573-5036
    Keywords: internode ; phloem ; rubidium ; solute transfer ; strontium ; temperature ; Triticum aestivum L. ; wheat ; xylem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat (Triticum aestivum L. cv. ‘Arina’) shoots grown in the field were excised post-anthesis and incubated in the laboratory for 72 h standing in 2 mM RbCl+2 mM SrCl2. Strontium is a phloemimmobile, xylem-mobile element and indicates the distribution of the xylem sap in the plant. Rubidium is easily transported in the phloem and behaves similarly to the highly mobile K as far as the redistribution within the plant is concerned, although Rb cannot substitute physiologically or biochemically for K. The Sr contents in the ear were hardly affected by stem length or by steam-girdling (phloem-interruption). Rubidium on the other hand accumulated in the stem. A peduncle length of 5 cm was sufficient to decrease the Rb concentration in the xylem by more than 50% at 25°C. Only a minor quantity of Rb reached the ear after passing through 20 cm of stem without nodes and this transport was prevented by steam-girdling. A remarkable flux of Rb into the ear was observed in shoots with a vascular connection between the flag leaf lamina and the ear. Our results suggest that Sr was transported with the transpiration stream, while Rb was rapidly eliminated from the xylem and reached the ear via the phloem. The temperature optimum for the removal of Rb from the xylem was around 35°C. The nodes may further contribute, but are not prerequisites for this redistribution. The observed transfer processes could allow a solute specific transport via the xylem and phloem of maturing cereals and may be an important factor influencing the nutrient economy in the field.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 98
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 25 (1991), S. 153-159 
    ISSN: 1573-5044
    Keywords: axillary bud culture ; germplasm maintenance ; growth inhibition ; mannitol ; minimal growth ; photoperiod ; sorbitol ; sucrose ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The need for conservation of biotic diversity is well recognized. However, improved techniques for the efficient, cost effective-preservation of plant germplasm are needed. The conservation and distribution of plant germplasm in vitro is gaining acceptance. However, increased usage is dependent upon the ability of curators to minimize culture maintenance requirements. This report examines the effect of various levels of sucrose, photoperiod, temperature, sorbitol and mannitol on minimal growth storage of Ipomoea batatas (L.) Lam. Growth was reduced 50% with a temperature reduction of from 21.1 to 15.6°C. Sucrose concentrations of 15 and 20 g l-1 resulted in reduced plant stature with few adverse effects on plantlet viability or morphology. Reduction of photoperiod from 16 to 4 h produced smaller, slightly chlorotic, but otherwise normal plants. The addition of sorbitol or mannitol to culture media generally produced undesirable effects on gross plant morphology and loss of apical dominance. Genotype x growth retarding treatment interactions were observed for all variables examined.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 99
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 91-92 (1982), S. 651-658 
    ISSN: 1573-5117
    Keywords: stream ; sediment ; sand ; hyporheal ; oxygen ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dissolved oxygen, pH, conductivity and alkalinity of surface and subsurface interstitial waters were investigated at Mill Creek (a small, rural, predominantly sandy stream in east Texas). Dissolved oxygen concentration tended to decrease with sediment depth, while conductivity and alkalinity did not significantly change with substrate depth. Surface water pH was significantly higher than interstitial water (p = 0.05). Chemical analyses of subsurface water from a pre- to post-storm event showed a depression in dissolved oxygen concentration in moderate and deep interstitial waters, immediately following the return of stream base flow, with the deeper strata returning to pre-storm oxygen levels four days later. A thermal convective current mechanism is proposed which would serve to transport surface water downward into these deeper interstices.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 100
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 86 (1982), S. 105-108 
    ISSN: 1573-5117
    Keywords: convection ; sediment ; temperature ; convectional diffusion ; material exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In two Finnish lakes, in winter and summer, a constant temperature in the sediment was not reached until a depth of 1.5–2 m. The thermal stratification pattern in the sediment was similar to that in water. However, the lack of turbulent mixing in the sediment resulted in a thin ‘episediment’. This stratification was caused by convection. Convection currents in the sediment were most prevalent during spring and autumn overturn when the density of overlying water was greater than that of the interstitial water. Convection was also possible in winter. The duration and magnitude of convection was dependent on warming and cooling rates. Such convection currents may be important in material exchange between sediment and water since material concentrations in interstitial water are 5–100 times greater than in overlying water.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...