Abstract
By pulse and chase labeling experiments, two independent mannoprotein pools have been found associated with the Saccharomyces cerevisiae envelope. One of them probably corresponds to mannoproteins localized in the periplasmic space. These molecules showed a high turnover rate at 28° C. The second pool is formed by intrinsic wall mannoproteins which are apparently stable for long periods of time, after a small initial turnover. These results suggest that at least part of the mannoproteins initially found in the periplasmic space may move into the wall.
The time lag between the addition of the radioactive precursors and their incorporation in the cell envelope (20–30 min for amino acids and about 10 min for carbohydrate) indicates that protein formation and carbohydrate incorporation take place in succession. Moreover, bulk glycosylation of mannoproteins seems to occur close in time to the moment of secretion into the periplasmic space.
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References
Arnold WN (1972) The structure of the yeast cell wall. Solubilization of a marker enzyme, β-fructofuranosidase, by the autolytic enzyme system. J Biol Chem 247:1161–1169
Ballou CE (1976) Structure and biosynthesis of the mannan component of the yeast cell envelope. Adv Microb Physiol 14:93–158
Cabib E, Bowers B (1971) Chitin and yeast budding. Location of chitin in yeast bud scars. J Biol Chem 246:152–159
Cabib E, Bowers B (1975) Timing and function of chitin synthesis in yeast. J Bacteriol 124:1586–1593
Cortat M (1971) Localisation intracellulaire et function des β(1→3) glucanases lors du bourgeonnement chez Saccharomyces cerevisiae. Thesis, Swiss Federal Institute of Technology, Zurich
Cortat M, Matile P, Wiemken A (1972) Isolation of glucanase-containing vesicles from budding yeast. Arch Microbiol 82:189–205
Del Rey F, Santos T, García-Acha J, Nombela C (1979) Synthesis of 1,3-β-glucanases in Saccharomyces cerevisiae during the mitotic cycle, mating and sporulation. J Bacteriol 139:924–931
Dunlop PC, Meyer GM, Ban D, Ron RJ (1978) Characterization of two forms of asparaginase in Saccharomyces cerevisiae. J Biol Chem 253:1297–1304
Elorza MV, Sentandreu R (1969) Effect of cycloheximide on yeast cell wall synthesis. Biochem Biophys Res Commun 36:741–747
Farkaš V (1979) Biosynthesis of cell walls of fungi. Microbiol Rev 43:117–144
Farkaš V, Kovařik J, Košinova A, Bauer Š (1974) Autoradiographic study of mannan incorporation into the growing cell walls of Saccharomyces cerevisiae. J Bacteriol 117:265–269
Gascón S, Neumann NP, Lampen JO (1968) Comparative study of the properties of the purified internal and external invertases from yeast. J Biol Chem 243:1573–1577
Gunja-Smith Z, Patil NB, Smith EE (1977) Two pools of glycogen in Saccharomyces. J Bacteriol 130:818–825
Kitamura K, Yamamoto Y (1972) Purification and properties of an enzyme, Zymolyase, which lyses viable yeast cells. Arch Biochem Biophys 153:403–406
Krátký Z, Biely P, Bauer Š (1975) Wall mannan of Saccharomyces cerevisiae. Metabolic stability and release into growth medium. Biochim Biophys Acta 404:1–6
Lazo PG, Ochoa AG, Gascón S (1977) α-Galactosidase from Saccharomyces carlsbergensis. Cellular location and purification of the external enzyme. Eur J Biochem 77:375–382
Linnemans WAM, Boer P, Elbers PF (1977) Localization of acid phosphatase in Saccharomyces cerevisiae: a clue to cell wall formation. J Bacteriol 131:638–644
Novick P, Ferro S, Schekman R (1981) Order of events in the yeast secretory pathway. Cell 25:461–469
Rodríguez L, Laborda F, Sentandreu R (1979) Patterns of wall synthesis in Saccharomyces cerevisiae. Current Microbiol 2:293–297
Rodríguez L, Ruiz T, Elorza MV, Villanueva JR (1980) Metabolic relationship between invertase and acid phosphatase isoenzymes in Saccharomyces cerevisiae. Biochem Biophys Acta 629:445–454
Ruiz-Herrera J, Sentandreu R (1975) Site of initial glycosylation of mannoproteins from Saccharomyces cerevisiae. J Bacteriol 124:127–133
Santos E, Sentandreu R (1981) Mannosyl transferases in Saccharomyces cerevisiae: evidence for the occurrence of ectomannosyltransferase activity. Current Microbiol 6:361–366
Sentandreu R, Elorza MV, Villanueva JR (1975) Synthesis of yeast wall glucan. J Gen Microbiol 90:13–20
Tronchin G, Poulain D, Herbaut J, Biguet J (1981) Cytochemical and ultrastructural studies of Candida albicans. II. Evidence for a cell wall coat using Concanavalin A. J Ultrastruct Res 75:50–59
Villanueva JR, Notario V, Santos T, Villa TG (1976) β-Glucanases in nature. In: Peberdy JF, Rose AH, Rogers HJ, Cocking EC (eds) Microbial and plant protoplasts. Academic Press, London New York, pp 323–358
Vrsanska M, Krátký Z, Biely P (1977) Lysis of intact yeast cells and isolated cell walls by an inducible enzyme system of Arthrobacter GJM-1. Z Allg Mikrobiol 17:391–402
Welten-Vestergen GW, Boer P, Steyn-Paevé EP (1980) Lipid-mediated glycosylation of endogenous proteins in isolated plasma membrane of Saccharomyces cerevisiae. J Bacteriol 141:342–349
Wessels JGH, Sietsma JH (1981) Fungal cell walls: a survey. In: Tanner W, Loewus FA (eds) Encyclopedia of plant physiology, New Series, Vol 13B. Springer, Berlin Heidelberg New York, pp 352–394
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Javier Pastor, F.I., Herrero, E. & Sentandreu, R. Metabolism of Saccharomyces cerevisiae envelope mannoproteins. Arch. Microbiol. 132, 144–148 (1982). https://doi.org/10.1007/BF00508720
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DOI: https://doi.org/10.1007/BF00508720