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1

Digitale Medien

Interspecific variation in sustained swimming ability of late pelagic stage reef fish from two families (Pomacentridae and Chaetodontidae) (1998)

Stobutzki, I. C.

Springer

Coral reefs 17 (1998), S. 111-119

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ISSN: 1432-0975

Schlagwort(e): Key words Dispersal ; coral reefs ; fish ; swimming ; Larvae ; Juveniles

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Geologie und Paläontologie

Notizen: Abstract Characteristics of the pelagic stages of reef fishes have generally been investigated at the family level, which may mask important differences among species. Here the variation in sustained swimming ability of the late pelagic stages is examined among species, within two families (Pomacentridae and Chaetodontidae). The pomacentrids displayed a 7.5-fold difference in sustained swimming ability across 24 species, while the chaetodontids displayed a 2-fold difference across 10 species. The variation within the Pomacentridae was not related to pelagic larval duration, post-settlement habitat or taxonomy. There was, however, a significant correlation between sustained swimming ability and total length (TL) of individuals (r=0.435, P〈0.0001). Differences in the mean distance swum by pomacentrid species, however, was most strongly related to differences in mean wet weight (r=0.814, P〈0.0001). When the mean distance swum by species was scaled with respect to mean TL there was still a strong correlation with mean wet weight (r=0.644, P〈0.005). Among chaetodontid individuals TL and sustained swimming ability were not correlated (r=−0.004, P=0.978). Furthermore, sustained swimming ability was not significantly related to the trans-oceanic distribution of species in either family. The variation in sustained swimming ability, however, may contribute to explanations of the observed levels of gene flow within populations.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s003380050104

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2

Digitale Medien

Estuarine reconnection of an impounded mangrove salt marsh in the Indian River Lagoon, Florida: short-term changes in fish fauna (1998)

Taylor, D. Scott ; Poulakis, Gregg R. ; Kupschus, Sven R. ; [weitere]

Springer

Mangroves and salt marshes 2 (1998), S. 29-36

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ISSN: 1572-977X

Schlagwort(e): culverts ; culvert trap ; mosquito impoundment ; seine ; fish

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Fish population dynamics in a 24.3 ha mangrove-dominated mosquito impoundment in east-central Florida were examined by seining and culvert traps before and after installation of culverts that established estuarine connection for the first time in 39 years. In a 27-day period following the culvert opening, fish species increased from 9 to 21, while total number of fish in the impoundment decreased. Movement of fishes through culverts in both directions commenced immediately following culvert opening. Recruitment of transient species into the impoundment appeared to key on a single wind-driven high tide event. Such short-term events may be important cues for fish movement into and out of impounded salt marshes.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1009969708038

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3

Digitale Medien

On the cellular localization of phosphoenolpyruvate carboxylase in Sorghum leaves (1981)

Perrot, Catherine ; Vidal, Jean ; Burlet, Arlette ; [weitere]

Springer

Planta 151 (1981), S. 226-231

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ISSN: 1432-2048

Schlagwort(e): Immunocytochemistry ; (PEP carboxylase) ; PEP carboxylase ; Sorghum

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00395173

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4

Digitale Medien

Subcellular localization of β-1,3-glucanase in Puccinia recondita f.sp. tritici-infected wheat leaves (1998)

Hu, Guanggan ; Rijkenberg, Frits H. J.

Springer

Planta 204 (1998), S. 324-334

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ISSN: 1432-2048

Schlagwort(e): Key words:β-1 ; 3-Glucanase ; Immunocytochemistry ; Leaf rust pathogen ; Resistance ; Triticum (pathogen resistance)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract. An antiserum raised against the purified 33-kDa β-1,3-glucanase of wheat (Triticum aestivum L.) was employed to investigate the ultrastructural localization of the enzyme in wheat leaves infected with Puccinia recondita Rob. ex Desm. f.sp. tritici Eriks. and Henn. using a post-embedding immunogold labelling technique. In both compatible and incompatible interactions, β-1,3-glucanase was detected in the host plasmalemma and in the domain of the host cell wall near the plasmalemma of the mesophyll cells, but higher concentrations of the enzyme were detected in infected resistant wheat leaves than in infected susceptible ones. β-1,3-Glucanase was also found in the secondary thickening of xylem vessels and in the walls of guard cells, epidermal cells and phloem elements, while no labelling was observed in host organelles, viz. vacuoles, mitochondria, endoplasmic reticulum, Golgi bodies, nuclei and chloroplasts. A low concentration of the enzyme was detected on the intercellular hyphal wall and in the hyphal cytoplasm. In the compatible interaction, β-1,3-glucanase was demonstrated to accumulate predominantly in the haustorial wall and extrahaustorial matrix. In the incompatible interaction, strong labelling for β-1,3-glucanase was found in host cell wall appositions, in the extracellular matrix in the intercellular space, and in electron-dense structures of host origin which occurred in the incompatible interaction only.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004250050263

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5

Digitale Medien

Cytological localization of α-MSH, ACTH and β-endorphin in the pars intermedia of the cichlid teleost Sarotherodon mossambicus (1981)

Eys, G. J. J. M. ; Oetelaar, P.

Springer

Cell & tissue research 215 (1981), S. 625-633

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ISSN: 1432-0878

Schlagwort(e): Teleost ; Pars intermedia ; Immunocytochemistry ; PAP-technique ; MSH ; ACTH ; Endorphin

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The pars intermedia of S. mossambicus contains two different endocrine-cell types. The predominant cell type is lead-haematoxyline-positive and assumed to synthesize MSH and related peptides. The second cell type is PAS positive and its function and product(s) are unknown. Staining of light-microscopic and ultrathin sections with antisera against α-MSH, ACTH 1–24 and human β-endorphin revealed that only the lead-haematoxyline-positive cells of the pars intermedia react with these antisera, and that the secretory granules of these cells contain compounds that were immunoreactive to all three antisera. These findings are in line with the hypothesis that α-MSH, ACTH and endorphins are derived from the same precursor molecule. No specific reaction with one of the antisera could be detected in the PAS positive cells.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00233536

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6

Digitale Medien

Evidence for somatostatin, gastrin and pancreatic polypeptide-like substances in the mucosa cells of the gut in fishes with and without stomach (1981)

Noaillac-Depeyre, J. ; Hollande, E.

Springer

Cell & tissue research 216 (1981), S. 193-203

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ISSN: 1432-0878

Schlagwort(e): Digestive tract ; Fish ; Gastrin-immunoreactive cells ; Pancreatic polypeptide-immunoreactive cells ; Somatostatin-immunoreactive cells ; Immunocytochemistry

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Gastrin, pancreatic polypeptide and somatostatin immunoreactive cells in the gut of two fish with stomachs (perch and catfish) and a stomachless fish (carp) were studied by immunocytochemistry. In the gastric mucosa of perch and catfish, cells showing gastrin and somatostatin-like immunoreactivity are found, scattered among the surface mucous cells and mucous neck cells. No pancreatic polypeptide (P.P.) immunoreactive cells are detected in the gastric mucosa. Cells showing gastrin and P.P.-like immunoreactivity are observed in the intestinal mucosa of perch, catfish and carp. In this location no somatostatin immunoreactive cells are found.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00234554

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7

Digitale Medien

Immunohistochemical localisation of substances reactive to antisera against α-and β-endorphin and met-enkephalin in the brain of Rana temporaria L. (1981)

Doerr-Schott, Jeannine ; Dubois, Maurice P ; Lichte, Christine

Springer

Cell & tissue research 217 (1981), S. 79-92

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ISSN: 1432-0878

Schlagwort(e): α- and β-Endorphins ; Met-enkephalin ; Brain ; Immunocytochemistry ; Rana temporaria (Amphibia, Anura)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary In the brain of Rana temporaria, two distinct systems reactive with α- and β-endorphin antisera, respectively, and with a met-enkephalin antiserum, have been detected immunohistochemically. Neurons reacting with α- and β-endorphin antisera are located (1) in the preoptic nucleus, and (2) in the pars ventralis of the tuber cinereum. Immunoreactive nerve fibres of both groups of perikarya end in the infundibular floor near the capillaries and the preoptico-hypophysial tract. Control reactions have shown that the immunoreactivity is suppressed by the corresponding antigens, but also by β-LPH. In view of these results the immunoreactive systems examined correspond to an α/β-endorphin system or a lipotropinergic system. Neurons reacting with the met-enkephalin antiserum are located in the paraventricular organ. Intense immunofluorescence was observed in the infundibular floor. Controls show that the labelling by met-enkephalin antiserum is exclusively suppressed by met-enkephalin. In the pituitary gland, on the other hand, α- and β-endorphin antisera reveal: 1) the MSH/ACTH-like cells of the pars intermedia and 2) the ACTH-like cells of the pars distalis.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00233828

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8

Digitale Medien

Monoclonal antibodies SMI 311 and SMI 312 as tools to investigate the maturation of nerve cells and axonal patterns in human fetal brain (1998)

Ulfig, N. ; Nickel, J. ; Bohl, J.

Springer

Cell & tissue research 291 (1998), S. 433-443

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ISSN: 1432-0878

Schlagwort(e): Key words: Neurofilaments ; Phosphorylation ; Differentiation ; Immunocytochemistry ; Brain storage ; Fixation ; Microwave ; Human

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract Neurofilaments, which are exclusively found in nerve cells, are one of the earliest recognizable features of the maturing nervous system. The differential distribution of neurofilament proteins in varying degrees of phosphorylation within a neuron provides the possibility of selectively demonstrating either somata and dendrites or axons. Non-phosphorylated neurofilaments typical of somata and dendrites can be visualized with the aid of monoclonal antibody SMI 311, whereas antibody SMI 312 is directed against highly phosphorylated axonal epitopes of neurofilaments. The maturation of neuronal types, the development of area-specific axonal networks, and the gradients of maturation can thus be demonstrated. Optimal immunostaining with SMI 311 and SMI 312 is achieved when specimens are fixed in a mixture of paraformaldehyde and picric acid for up to 3 days and sections are incubated free-floating. Neurons, with their dendritic domains immunostained by SMI 311 in a Golgi-like manner, can be completely visualized in relatively thick sections. The limitations of Golgi-preparations, such as glia-labeling, artifacts, and the staining of only a small non-representative percentage of existing neurons, are not apparent in SMI preparations, which additionally provide the possibility of selectively staining axonal networks. The results achieved in normal fetal brain provide the basis for studies of developmental disturbances.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004410051013

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9

Digitale Medien

Immunocytochemical localization of annexin 5, a calcium-dependent phospholipid-binding protein, in rat endocrine organs (1998)

Kawaminami, M. ; Kawamoto, Takayuki ; Tanabe, Teijiro ; [weitere]

Springer

Cell & tissue research 292 (1998), S. 85-89

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ISSN: 1432-0878

Schlagwort(e): Key words Annexin 5 ; Immunocytochemistry ; Pituitary ; Ovary ; Testis ; Adrenal gland ; Thyroid gland ; Rat (wistar)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract Annexin 5, a unique calcium- and phospholipid-binding protein, has been investigated for its specific distribution in rat endocrine organs by immunocytochemistry with a specific antiserum to recombinant rat annexin 5. Follicular epithelial cells and parafollicular cells of the thyroid gland, adrenocortical cells of the zona fasciculata and zona reticularis, luteal cells, testicular interstitial cells, and Sertoli cells were shown to contain annexin 5. To examine whether the synthesis of annexin 5 would be affected by a change in humoral signal, the distribution of annexin 5 in the anterior pituitary was examined three weeks after ovariectomy. The withdrawal of ovarian hormones induced huge castration cells in the anterior pituitary gland, which contained abundant annexin 5. Annexin 5 was not detected in the pineal gland, the parathyroid gland, the islet of Langerhans, the adrenal medulla, zona glomerulosa cells, and granulosa cells. Since annexin 5 was shown to exist in many of the endocrine tissues examined, to be localized in specific cell types, and to be abundant in castration cells, it is suggested that annexin 5 contributes to secretory cell functions, which may be common to endocrine cells secreting chemically different hormones.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004410051037

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10

Digitale Medien

Two differing salmon GnRH precursor mRNAs are co-expressed in the brain of sockeye salmon (Oncorhynchus nerka) (1998)

Amano, M. ; Ashihara, Moto-oki ; Yoshiura, Yasutoshi ; [weitere]

Springer

Cell & tissue research 292 (1998), S. 267-273

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ISSN: 1432-0878

Schlagwort(e): Key words Co-expression of mRNAs ; Gonadotropin-releasing hormone ; Immunocytochemistry ; In situ hybridization ; Oncorhynchus nerka (Teleostei)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract The localization of two salmon-type gonadotropin-releasing hormone (sGnRH) precursors, pro-sGnRH-I (short type) and pro-sGnRH-II (long type), was investigated by using in situ hybridization techniques in the brain of the landlocked sockeye salmon, Oncorhynchus nerka. We used 30-mer oligonucleotide probes complementary to pro-sGnRH-I and pro-sGnRH-II cDNA. No significant differences were observed in the localization of sGnRH neurons expressing pro-sGnRH-I and pro-sGnRH-II mRNAs; both were expressed in the olfactory nerve, the olfactory bulbs, the regions between the olfactory bulb and telencephalon, the ventral telencephalon, the preoptic area, and the hypothalamus. Almost all sGnRH neurons examined co-expressed both precursors. The expression of two sGnRH precursors in the same neuron and the wide distribution of such neurons in the brain suggest that there are no functional differences between the two precursors.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004410051057

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