Publication Date:
1991-05-03
Description:
A human immunoglobulin G1 (IgG1) antibody oligomer was isolated from a transfected myeloma cell line that produced a monoclonal antibody to group B streptococci. Compared to the IgG1 monomer, the oligomer was significantly more effective at protecting neonatal rats from infection in vivo. The oligomer was also shown to cross the placenta and to be stable in neonatal rats. Immunochemical analysis and complementary DNA sequencing showed that the transfected cell line produced two distinct kappa light chains: a normal light chain (Ln) with a molecular mass of 25 kilodaltons and a 37-kilodalton species (L37), the domain composition of which was variable-variable-constant (V-V-C). Cotransfection of vectors encoding the heavy chain and L37 resulted in production of oligomeric IgG.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shuford, W -- Raff, H V -- Finley, J W -- Esselstyn, J -- Harris, L J -- New York, N.Y. -- Science. 1991 May 3;252(5006):724-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immune Sciences, Bristol-Myers Squibb Pharmaceutical Research Institute-Seattle, WA 98121.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1902593" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Animals, Newborn
;
Antibodies, Bacterial/biosynthesis/immunology/pharmacokinetics
;
Antibodies, Monoclonal/biosynthesis/immunology/pharmacokinetics
;
Cell Line
;
Female
;
Humans
;
Immunization, Passive
;
Immunoglobulin G/*biosynthesis/genetics/immunology
;
Immunoglobulin M/genetics
;
Immunoglobulin Variable Region/*biosynthesis/genetics/immunology
;
Immunoglobulin kappa-Chains/*biosynthesis/genetics/immunology
;
Macromolecular Substances
;
Maternal-Fetal Exchange
;
Multiple Myeloma
;
Pregnancy
;
Rats
;
Streptococcal Infections/prevention & control
;
Streptococcus agalactiae/immunology
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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