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  • Articles  (16)
  • Phytochrome  (16)
  • Springer  (16)
  • Blackwell Publishing Ltd
  • Oxford University Press
  • 1975-1979  (16)
  • 1979  (16)
  • Biology  (16)
Collection
  • Articles  (16)
Publisher
  • Springer  (16)
  • Blackwell Publishing Ltd
  • Oxford University Press
Years
  • 1975-1979  (16)
Year
Topic
  • Biology  (16)
  • 1
    ISSN: 1432-2048
    Keywords: Citrate synthase ; Fat degradation ; Isocitrate lyase ; Malate synthase ; Phytochrome ; Sinapis ; Starch (transitory)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The degradation of storage fat in the cotyledons of mustard seedlings is unaffected by phytochrome and photosynthesis (irradiation with continuous red or far-red light from sowing of the seeds) although light imposes a strong constraint on the translocation of organic matter from the cotyledons into the seedling axis. Likewise, the development and disappearance of glyoxysomal enzyme activities (isocitrate lyase, malate synthase, citrate synthase) takes place independently of light. It is concluded that the mobilization of storage fat (fat→carbohydrate transformation) is independent of photomorphogenesis. The surplus of carbohydrate produced from fat in the light seems to be converted to starch grains in the plastids, which function as a secondary storage pool in the cotyledons.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 146 (1979), S. 31-39 
    ISSN: 1432-2048
    Keywords: Autoradiography ; Calcium ; Mougeotia ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ca2+ is proposed to function as a messenger in such phytochrome-mediated responses as localized cell growth, intracellular movements, and control of plasma membrane properties. To test this hypothesis, the uptake of Ca2+ in irradiated and non-irradiated regions of individual threads of the green alga Mougeotia was studied with the aid of 45Ca2+ and low temperature autoradiography: 10–20 cells within 40–60 cell-long threads were irradiated for up to 1 min, transferred to darkness for 3 to 10 min, submersed in a radioactive medium for 1 min, washed in an unlabelled medium for 30 min, and then autoradiographed at-80° C for several days. The autoradiographs show that those cells which had been pre-irradiated with red light did take up 2–10 times more Ca2+ than the adjacent non-irradiated cells of the same thread. Cells pre-irradiated with farred light or red light followed by far-red light showed no enhanced uptake of Ca2+. These results might be interpreted to indicate, firstly, that phytochrome-Pfr is involved in the enhanced uptake of Ca2+ and secondly, that the accumulation of radioactive Ca2+ in red light irradiated cells is an expression of an increased intracellular concentration of Ca2+. This interpretation is based on the data that (i) the dark interval between irradiation and labelling precluded the involvement of photosynthesis, (ii) the effect of red light was reversible with far-red light, and (iii) the accumulation of Ca2+ persisted during the long wash-out period. We speculate, that the red light-enhanced accumulation of Ca2+ in Mougeotia cells is caused by a Pfr-mediated increase of the Ca-permeability of the plasma membrane, and perhaps by a Pfr-impeding of an active Ca2+-extrusion.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Planta 147 (1979), S. 229-235 
    ISSN: 1432-2048
    Keywords: Avena ; Greening ; Mitochondria ; Oxidative Phosphorylation ; Phytochrome ; Respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondria isolated from 7-day old darkgrown Avena sativa L. (var. Arnold) laminae given 5 min illumination of red light, followed by varying lengths of darkness up to 3 h, showed at least a twofold increase in the rates of both NADH-dependent oxygen consumption and respiratory chain phosphorylation over those of mitochondria isolated from unilluminated tissue. Similar organelles, isolated from tissue given either far-red or red followed by far-red pretreatment, exhibited rates of both functions of between 25% and 75% below those of the mitochondria from unilluminated tissue. The induction-reversion criteria for phytochrome control of respiration and oxidative phosphorylation were satisfied under all experimental conditions during the greening process. Treatment with continuous far-red light, acting presumably through the ‘high irradiance’ reaction of phytochrome, served to disengage phytochrome activity from photosynthesis. The stimulation of oxidative phosphorylation still occurred under these conditions, slightly slower but much more prolonged in the absence of ATP from photophosphorylation.
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  • 4
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    Springer
    Planta 145 (1979), S. 405-409 
    ISSN: 1432-2048
    Keywords: Chenopodium ; Internode elongation ; Photoreception sites ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The elongation of the fourth internode of ‘fully green’ Chenopodium polyspermum L. is modulated by far-red light (FR) given in addition to the main light period. Two different types of organs are responsible for the photoreception of FR producing the end-of-day effect; the stem and the leaves situated just above and below the reacting internode. Photoreversibility can be obtained within certain limits in the two organs. Evidence is presented which shows that in the ‘fully green’ plant there is an interorgan reaction whose primary reaction is the photoconversion of phytochrome.
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  • 5
    ISSN: 1432-2048
    Keywords: Carotenoids ; Chlorophylls ; Herbicides ; Phytochrome ; Plastid development ; Ribulosebisphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Treatment of the mustard (Sinapis alba L.) seedling with the herbicide SAN 9789 inhibits synthesis of colored carotenoids and interferes with the formation of plastid membrane lipids without affecting growth and morphogenesis significantly. In farred light, which is hardly absorbed by chlorophyll, development of plastid ultrastructure, synthesis of ribulosebisphosphate carboxylase and synthesis of chlorophyll are not affected by SAN 9789. It is concluded that normal phytochrome actions on plastid structural development, protein and chlorophyll syntheses are not affected by the absence of carotenoids provided that there is no significant light absorption in chlorophyll. The findings show that the inhibition of synthesis of one set of plastid membrane components (the carotenoids) does not stop synthesis of other components such as chlorophyll and does not halt membrane assembly. Supplementary experiments with the closely related compound SAN 9785, which affects the amount and composition of plastid lipids but not carotenoid and chlorophyll syntheses, suggest that the effect of the herbicide SAN 9789 is due exclusively to its inhibition of synthesis of colored carotenoids. In the presence of SAN 9789 white or red light at high fluence rate causes photodestruction of chlorophyll and ribulosebisphosphate carboxylase and photodecomposition of thylakoids. These effects are interpreted as resulting exclusively from the self-photooxidation and photosensitizing action of chlorophyll once the protection by carotenoids of chlorophyll against self- and sensitized photooxidation is lost.
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  • 6
    ISSN: 1432-2048
    Keywords: Phytochrome ; Development habitat ; Natural radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey of the responsiveness of plant species, typical of open and shade habitats, to simulated natural shade-light quality (i.e. white light plus supplementary far-red) has demonstrated a systematic relationship between habitat and certain developmental responses. Supplementary far-red light has a much greater effect on stem extension rate, petiole length, and leaf dry weight: stem dry weight ratio of the open habitat, shade-intolerant species. Far-red effects on leaf chlorophyll content show no such systematic grading. These results are discussed in relation to habitat adaptation. In most cases, the relationship between developmental response and the estimated phytochrome photoequilibrium, which is established by the radiation treatment, is linear. This is taken as an indication of phytochrome involvement in shade perception.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Planta 145 (1979), S. 269-272 
    ISSN: 1432-2048
    Keywords: Flowering ; Gibberellin ; Photoperiodism ; Phytochrome ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Combinations of far-red light (FR) (4 min) and gibberellic acid (GA3), given at the beginning of a daily 12-h dark period in a growth room, were used to study floral induction in four maturity genotypes of the milo group of sorghum (Sorghum bicolor (L.) Moench). The 12-h dark period without GA3 application or FR induced flowering in only the early genotype; FR hastened initiation in the early genotype, while GA3 hastened floral initiation in the two intermidiate-flowering genotypes. GA3 and FR together had a strong synergistic effect, hastening floral initiation by 30 to more than 80 d in the early and intermediate genotypes. Red light (R) did not hasten flowering; FR preceded by R gave the same effect as FR alone. GA3 promoted stem elongation equally whether floral initiation occurred or not; thus, its effect on stem elongation was independent of floral initiation. The capacity of GA3 to induce flowering in sorghum, a short-day plant, seems to be enhanced by phytochrome being in the PR form at the beginning of the night when GA3 was applied.
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  • 8
    ISSN: 1432-2048
    Keywords: Action spectrum ; Dormancy (seeds) ; Germination (seeds) ; Lactuca ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light of wavelengths in the range 400–500 nm induces germination of seeds of Lactuca sativa L. (cv. Noran and May Queen) when made light requiring by high temperature (thermodormancy) or prolonged exposure to far red light (far red dormancy). TD seeds proved more sensitive to blue light than FRD seeds by several orders of magnitude. Blue induced germination could be reversed by far red. Fluence response curves for this reversion are less steep for TD than for FRD seeds. The degree of germination of TD seeds after moderate doses of blue light is decreased by dark storage at 22° C or by exposure to far red and this is accompanied by a marked change in the shape of the fluence response curves. In these respects, germination induction by blue resembles that by red light. Continuous exposure to blue light inhibits germination of TD seeds but hardly that of FRD seeds. An action spectrum is given for blue induced germination of TD seeds.
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  • 9
    ISSN: 1432-2048
    Keywords: Action spectra ; Dormancy (seeds) ; Germination (seeds) ; Lactuca ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluence response curves for red light-induced germination of thermodormant (TD) seeds of Lactuca sativa L. show two regions that differ in their light sensitivity. In the region of high sensitivity, the germination responses differ between seed batches and can be altered by dark storage or far red irradiation. Induction of germination in far red dormant (FRD) seeds requires far higher fluences. Action spectra for induction to 60% germination were determined for these various response types. Spectra for the regions of low sensitivity response are similar for TD and FRD seeds. In comparison, the action spectrum for the highly sensitive response in TD seeds is significantly shifted to longer wavelengths. Analogous differences exist in the action spectra for far red reversal of the red induced germination responses. Germination induction in the low sensitivity region shows repeated red-far red reversibility. Far red reversal of red induction in the high sensitivity region does not saturate even at the highest far red fluences available and requires increased red fluences for subsequent reinduction. A model quantitatively accounting for these observations is presented. It is pointed out that action spectra of processes involving photoreversible pigments with partly overlapping absorption spectra in general are not identical with the absorption spectra of the partners. They should depend upon the degree of phototransformation required to elicit a given physiological response. In the case of induction of lettuce seed germination the observed action spectra can be interpreted as reflecting different requirements for P fr of the various response types. Our results do not necessitate the assumption of spectroscopically different forms of phytochrome in these seeds.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Planta 146 (1979), S. 75-81 
    ISSN: 1432-2048
    Keywords: Adenosine-5′-triphosphate ; Membrane ; Phaseolus ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a membrane fraction isolated from hypocotyls of Phaseolus aureus Roxb. the activity of a number of enzymes was regulated by red and far-red irradiation in vitro, provided that the tissue received a brief red light treatment before extraction. Other enzymes showed no photoregulation. There were two types of photocontrol, neither of which could be detected in the solute fraction, nor in extracts from completely etiolated material. One (Type I) was a red/far-red reversible regulation of the rate of enzyme activity, depending on the light given (in vivo or in vitro) before the assay was begun. The second (Type II) was a promotion of enzyme activity by red or far-red light given during the assay. The action spectra for type II responses do not coincide with either the phytochrome absorption or difference spectra. However, the effectiveness of red and far-red was correlated with the Pfr/P ratio present at the beginning of the assay, such that far-red was more efficient at high Pfr/P and red at low Pfr/P ratios. All enzymes that were regulated involved ATP. In samples that showed enzyme regulation, small changes in fluorescence yield of tryptophan and the covalent probe “Fluram” (Roche) accompanied the photoconversion of phytochrome, but no fluorescence changes could be measured after briefly incubating the membrane fraction with ATP. The results indicate that light may affect the interaction of ATP with the membrane fraction.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Planta 147 (1979), S. 1-5 
    ISSN: 1432-2048
    Keywords: Polyribosomes ; Phytochrome ; Raphanus ; Ribonucleases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The total ribosome content of radish cotyledons increases during the first 2–3 days of germination both in darkness and under far-red light irradiation; ribonuclease activity is not under phytochrome control during this period. Changes in ribonuclease activity interfere with the analysis of the polyribosomal population. A maximal ratio of polysomes to monosomes is observed 12 h after the onset of far-red light and then it decreases. A 12 h far-red irradiation stimulates the in vivo incorporation of amino acids into proteins. This stimulation persists when seedlings are transferred for 4 h to the dark.
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  • 12
    ISSN: 1432-2048
    Keywords: Cell wall ; Cytoplasm ; β-Fructosidase (transfer) ; Phytochrome ; Raphanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The far-red absorbing form of phytochrome, Pfr, rapidly increases the rate of transfer of β-fructosidase (E.C.3.2.1.26) from the cytoplasm to the cell wall in radish hypocotyls. Far-red light increases the level of enzyme in a particulate fraction: after two hours of light treatment, the particulate enzyme is associated almost exclusively with the endoplasmic reticulum. Transfer from the endoplasmic reticulum to the cell wall involves an incorporation into Golgi bodies and the plasmalemma: these membrane fractions were separated by centrifugation on a discontinuous sucrose density gradient and their degree of purity was determined by the use of known biochemical markers. With respect to β-fructosidase, light controls, via Pfr: (1) the total amount, (2) the incorporation into the endoplasmic reticulum and (3) the transfer to the cell-wall. These three processes have different sensitivities to cycloheximide.
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  • 13
    ISSN: 1432-2048
    Keywords: Anthocyanin ; Mode of action of phytochrome ; Phenylalanine ammonia-lyase ; Phytochrome ; Sinapis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction by light of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and of anthocyanin in cotyledons of the mustard (Sinapis alba L.) seedling is strongly affected by a light pretreatment which operates through phytochrome. If PAL or anthocyanin is induced by a light pulse, the effectiveness of phytochrome (Pfr) is strongly increased by a light pretreatment; however, if the increase of the PAL level or synthesis of anthocyanin is elicited by continuous far-red light (operating via phytochrome in the ‘High Irradiance Response’), effectiveness of light is strongly reduced by the same light pretreatment. This reduction of effectiveness is correlated with a decrease of total phytochrome (Ptot) caused by the light pretreatment. It is argued that the observations are compatible only with the ‘open phytochrome-receptor model’ as suggested by Schäfer (J. Mathem. Biol. 2, 41–56, 1975). The peaks of the time courses of the PAL levels under continous far-red light are located at 48 h after sowing and do not depend on the original level of phytochrome. The decrease of the PAL levels beyond 48 h after sowing takes place independently of phytochrome and of the actual level of PAL.
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  • 14
    Electronic Resource
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    Planta 146 (1979), S. 415-417 
    ISSN: 1432-2048
    Keywords: Germination (seeds) ; Osmotic stress ; Phytochrome ; Seed germination ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize caryopses sown in water germinate equally well either in darkness or under any light regime. However, when they are imbibed in mannitol solutions, continuous far-red light proves to be strongly inhibitory on the final germination as compared to darkness. Similar but less pronounced inhibition is also exhibited by continuous red or blue light. Intermittent far-red light can partially substitute for continuous far-red light in inhibiting maize caryopsis germination, and its effect is reversed to the intermittent red light level when red light is given immediately after each far-red illumination. These results are interpreted as a proof of existence and involvement of phytochrome in the germination control of maize caryopses, though its manifestation is realized only under osmotic stress.
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  • 15
    Electronic Resource
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    Springer
    Planta 146 (1979), S. 545-550 
    ISSN: 1432-2048
    Keywords: Blue light ; Cucumis ; Hypocotyl growth ; Lactuca ; Lycopersicon ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of blue light on hypocotyl extension in de-etiolated seedlings of lettuce, cucumber and tomato was investigated under conditions which precluded the involvement of phytochrome. Small but highly inhibitory amounts of blue light were added to a high intensity background illumination from low pressure sodium lamps. A log-linear response for inhibition of hypocotyl extension against the blue light fluence rate was obtained for lettuce and cucumber, and inhibition in tomato was also related to the blue light fluence rate. The added blue light did not alter phytochrome photostationary state and its effect was independent of the total fluence rate. Growth inhibition by Pfr could be demonstrated in tomato and cucumber but not in lettuce. The results indicate that two photoreceptors may normally be involved in the control of seedling growth but their relative importance varies greatly between species.
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  • 16
    ISSN: 1432-2048
    Keywords: Chenopodium ; Day light fluence rate ; End-of-day effect ; Internode elongation ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The elongation of the fourth internode of fully green Chenopodium polyspermum L. is strongly stimulated by far-red light (FR) given at the end of the day. The end-of-day effect is more important when the plants had been cultivated for several days with a main light period of 140 Wm-2 than with a main light period of 85 Wm-2. There exists a quantitative relationship between the FR end-of-day effect mediated by phytochrome and the value of the light fluence during the day.
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