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  • Volumetrie  (15)
  • Nitrogen fixation
  • Springer  (19)
  • American Chemical Society (ACS)
  • National Academy of Sciences
  • 1980-1984
  • 1975-1979  (19)
  • 1977  (19)
Collection
Publisher
  • Springer  (19)
  • American Chemical Society (ACS)
  • National Academy of Sciences
Years
  • 1980-1984
  • 1975-1979  (19)
Year
  • 1
    ISSN: 1432-2048
    Keywords: Leguminosae ; Nitrate-reductase mutants ; Nitrogen fixation ; Nodulation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of nitrate on the symbiotic properties of nitrate-reductase-deficient mutants of a strain of cowpea rhizobia (32H1), and of a strain of Rhizobium trifolii (TA1), were examined; the host species were Macroptilium atropurpureum (DC.) Urb. and Trifolium subterraneum L. Nitrate retarded initial nodulation by the mutant strains to an extent similar to that found with the parent strains. It is therefore unlikely that nitrite produced from nitrate by the rhizobia, plays a significant role in the inhibition of nodulation by nitrate. Nitrite is an inhibitor of nitrogenase, and its possible production in the nodule tissue by the action of nitrate reductase could be responsible for the observed inhibition of nitrogen fixation when nodulated plants are exposed to nitrate. However, the results of this investigation show that nitrogen fixation by the plants nodulated by parent or mutant strains was depressed by similar amounts in the presence of nitrate. No nitrite was detected in the nodules. Nodule growth, and to a lesser extent, the nitrogenase specific activity of the nodules (μmol C2H4g−1 nodule fr. wt. h−1), were both affected by the added nitrate.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 114 (1977), S. 197-201 
    ISSN: 1432-072X
    Keywords: Anabaena sp. ; Nitrogen fixation ; Heterocysts ; Cyanobacteria ; Marine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A marine filamentous cyanobacterium capable of rapid growth under N2-fixing conditions has been isolated from the Texas Gulf Coast. This organism appears to be an Anabaena sp. and has been given the strain designation CA. Cultures grown on mineral salts medium bubbled with 1% CO2-enriched air at 42°C show a growth rate of 5.6±0.1 generations per day with molecular nitrogen as the sole nitrogen source. This growth rate is higher than any other reported in the literature to date for heterocystous cyanobacteria growing on N2. Under similar growth conditions, 7.5 mM NH4Cl yields a growth rate of 6.6±0.1 generations per day while 7.5 mM KNO3 allows for a growth rate of 5.8±0.4 generations-day. Nitrogen-fixation rates, as measured by acetylene reduction, show maximum activity values in the range of 50–100 nmoles ethylene produced/minxmg protein. These values compare favorably with those obtained from heterotrophic bacteria and are much higher than values reported for other cyanobacteria. Growth experiments indicate that the organism requires relatively high levels of sodium and grows maximally at 42°C. Because of its high growth rate on N2, this newly isolated organism appears ideal for studying nitrogen metabolism and heterocyst development among the cyanobacteria.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 114 (1977), S. 43-49 
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Anabaena ; Hydrogenase ; Hydrogen-uptake ; Nitrogenase ; Nitrogen fixation ; Protection mechanism ; Inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The blue-green alga Anabaena cylindrica is found to consume molecular hydrogen in a hydrogenase dependent reaction. This hydrogen uptake proceeds in the dark and is strictly dependent on oxygen, thus representing a Knallgas reactions. Its rate is almost as high as that of the endogenous respiration in Anabaena. Studies with inhibitors reveal that hydrogen is utilized via the complete respiratory chain providing additional energy for the alga. CO plus C2H2 completely block the Knallgas reaction which explains the previously reported considerable increase in the total H2 formation representing the difference between the nitrogenase-dependent H2-evolution and the reutilization of the gas catalysed by the hydrogenase in intact Anabaena. H2 is able to support the C2H2-reduction in the dark in a reaction again strictly dependent on oxygen. Moreover, H2 is also consumed in experiments carried out under far red light and in the presence of dichlorophenyl-dimenthyl-urea (DCMU) where the energy for nitrogen fixation is no longer provided by respiration but by cyclic photophosphorylation. Under these conditions, H2 is found to supply electrons for the formation of C2H4 from C2H2 in a reaction no longer dependent on the presence of oxygen. Moreover, in these experiments, the presence of H2 stabilizes the C2H2-reduction activity against the deleterious effect of oxygen. Thus, this communication provides evidence for a triplicate function of the H2-uptake catalysed by hydrogenase in intact Anabaena which is (a) to provide energy by the Knallgas reaction, (b) to supply reducing equivalents for nitrogenase, (c) to protect nitrogenase from damage by oxygen.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 56 (1977), S. 273-279 
    ISSN: 1573-5117
    Keywords: Nitrogen fixation ; nutrient enrichment ; limnocorral
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrogen fixation was measured in situ by the 15N tracer technique in the Bay of Quinte, Lake Ontario, and three lake enclosures with different nutrient enrichment. The fixation rates in the Bay were low but detectable during the summer season. The fixation activities were found to be correlated with the presence of nitrogen-fixing blue-green algae and the distribution of the algal species in the water was affected by nitrate enrichment. The study showed that, with the addition of nitrate, species not able to fix atmospheric nitrogen became predominant. However, in the absence of external nitrogen (i.e., nitrate), species able to fix nitrogen became dominant. Phosphorus enrichment alone did result in higher N-fixation rates in the water and, without the addition of phosphorus, the fixation rates are lower and fluctuate throughout the season, presumably dependent on the availability of phosphorus in the water. A comparison between the 15N-isotopic method and the acetylene reduction method is reported and the factors involved in the variations between these two methods are discussed.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 283 (1977), S. 301-301 
    ISSN: 1618-2650
    Keywords: Verw. von Oxazinfarbstoffen als Indicatoren ; Volumetrie ; Cerat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 284 (1977), S. 43-43 
    ISSN: 1618-2650
    Keywords: Best. von Kobalt(II), Nickel(II), Mangan(II), Silber(I) mit ÄDTA ; Volumetrie ; Glyoxal-bis-(2-hydroxyanil) als Indicator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 284 (1977), S. 43-43 
    ISSN: 1618-2650
    Keywords: Best. von Natriumthiosulfat mit Bromsuccinimid ; Volumetrie ; Bordeauxrot als Indicator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 285 (1977), S. 125-125 
    ISSN: 1618-2650
    Keywords: Best. von Arsen(III) mit Azinfarbstoffen als Indicatoren ; Volumetrie ; Bromat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 285 (1977), S. 126-126 
    ISSN: 1618-2650
    Keywords: Best. von Gallium, Gadolinium in Granaten ; Volumetrie ; ÄDTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 283 (1977), S. 269-273 
    ISSN: 1618-2650
    Keywords: Best. von Metallionen ; Volumetrie ; ÄDTA, alkalimetrische Methode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Mit der weiterentwickelten alkalimetrisch-komplexometrischen Methode können auch selektive Bestimmungen und Trennungen von Metallionen durchgeführt werden. Zur Anwendung kommen Fällungs- und Maskierungsmittel (K2CrO4, KJ, NaF, NH4CNS u.a.) und Titrationen bei verschiedenen pH-Werten. Nach einer Vorneutralisation wird die Bestimmung potentiometrisch mit einem Titrigraphen oder pH-Meter gegen die Glaselektrode durchgeführt. Die relative Standardabweichung liegt meist zwischen ±0,3 und ±0.5%.
    Notes: Abstract With the further development of the alkalimetric-complexometric method the accomplishment of selective determinations and separations of metal ions has become possible. Masking agents (K2CrO4, KI, NaF, NH4CNS and others) and titrations under different pH values are being applied. After a preliminary neutralization the determination is carried out potentiometrically with the aid of a titrigraph or pH-meter and the glass electrode. The relative standard deviation is mostly between ±0.3% and ±0.5%.
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