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  • Spektralphotometrie  (30)
  • Rat  (24)
  • Springer  (54)
  • 1975-1979  (54)
  • 1976  (54)
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  • Springer  (54)
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  • 1975-1979  (54)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 171 (1976), S. 285-296 
    ISSN: 1432-0878
    Keywords: Prostate ; Rat ; Castration ; Nuclear alterations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the nuclei of epithelial cells of the dorsal lobe of the rat prostate were studied 2, 3, 5, 7 and 21 days after castration. The nucleolus appears to undergo a progressive disorganisation with partial fragmentation and dispersion of its normal components. Changes in the nucleoplasm were primarily reflected by a condensation of chromatin, particularly along the nuclear membrane and adjacent to the nucleolus. Later, different types of intranuclear inclusions were observed. After 21 days, the nuclei were characterized by an irregular outline with large indentation. Within the nucleoplasm aggregates of coarse granular chromatin were found. No cell necrosis was observed, indicating that androgen deprivation results in a remodeling of the cell to a less active state with marked cellular alterations and cessation of secretion, but apparently with some of their basic functions still intact. Injections of testosterone completely reverse the castrated-induced alterations. The changes observed are assumed to be due to the withdrawal of the androgenic stimulus, with a direct influence on the secretory function of the cell. The findings support the view that the stimulating secretory effect of androgen is mediated via an intranuclear androgen receptor, probably located in the nucleolus-associated-chromatin. It is also proposed that the secretory function of the epithelial cells of the prostatic complex, initiated by androgens, may be regulated by an intranuclear secretory center.
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  • 2
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    Cell & tissue research 173 (1976), S. 529-542 
    ISSN: 1432-0878
    Keywords: Capillaries ; Cerebral cortex ; Endothelial cells ; Intraendothelial lumen ; Interendothelial lumen ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Developing blood vessels in rat cerebral cortex were studied at a number of stages between 3 and 28 days postnatal, in an attempt to obtain data on the mechanisms by which the lumen is established within cords of mesodermal cells. A combination of techniques was utilized in an attempt to elucidate these mechanisms. These were: (a) aldehyde fixation and block staining with phosphotungstic acid; (b) aldehyde perfusion followed by perfusion of a lead solution and post-fixation in osmium tetroxide; (c) conventional preparation of tissue with aldehyde and osmium fixation. Support for interendothelial lumen formation was readily forthcoming, including vessels with junctions between two or more endothelial cells cut transversely. There was some support for intraendothelial lumen formation, in the form of “seamless” endothelial cells. Other features noted included the presence of free ribosomes and vacuoles in the endothelial cells, endothelial flaps, sprouts and tendrils, intraluminal debris, endothelial degeneration and a junction with a nonendothelial cell. Large numbers of endothelial vacuoles were noted, many of them occurring at the abluminal edge of the cells. These vacuoles may be involved in the formation of intraendothelial lumina and also in the enlargement of both types of lumina. This study provides evidence that besides the well-established inter-endothelial lumen formation, intraendothelial mechanisms may also be operative in rat cerebral cortex. The techniques employed in this study offer the potential for clarifying these and related issues.
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  • 3
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    Cell & tissue research 168 (1976), S. 261-270 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Exercise ; Rat ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Four groups of male rats were exercised for periods of 2, 4, 6, and 8 weeks with controls in each group. As a result of chronic exercise there was an increase in the width of the zona fasciculata of the adrenal cortex. Also, there was an increase in the number and size of the mitochondria, and an increase in the quantity of smooth endoplasmic reticulum, and during the first 4 weeks of exercise an increase in the number of lipid droplets in the zona fasciculata. The close relationship between the smooth endoplasmic reticulum and the mitochondria, and the relationship between the smooth endoplasmic reticulum and the lipid droplets suggests a possible means for a transport mechanism for movement of precursors between these organelles.
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  • 4
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    Cell & tissue research 168 (1976), S. 303-314 
    ISSN: 1432-0878
    Keywords: Supraependymal cells ; Third ventricle ; Hypothalamus ; Hypophyseotropic area ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The supraependymal cells (SEC) are a normal component of the wall of the cerebral ventricles. In the hypothalamic area of the third ventricle they are restricted, in healthy animals, to the ependymal projection of the hypophyseotropic area. Here the SEC show great polymorphism. In addition to bipolar, multipolar and stellate or spider-like cells, transitional forms between these types can be seen. Their perikarya and processes can either remain at some distance from the ependyma or be in close contact with it. The processes may protrude between the ependymal cells or show surface differentiations that resemble the thin cytoplasmic folds of the mesenchymal wandering cells. Considering this and the variations in the number of cells, for example during the ovarian cycle, the SEC can be interpreted as mesenchymal cells, probably related to microglial cells of the subependymal layer. It is suggested that the SEC have a phagocytotic function and may be involved in the normal renewal of the ependyma. A definitive explanation for the restriction of the SEC to the hypophyseotropic area as well as the elucidation of their function remain to be found.
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  • 5
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    Cell & tissue research 168 (1976), S. 117-131 
    ISSN: 1432-0878
    Keywords: Retino-hypothalamic projection ; Suprachiasmatic nucleus ; Rat ; Axonal iontophoresis/cobalt precipitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The technique of cobalt sulfide precipitation combined with Timm's sulfide-silver method for intensification of heavy metals was used to delineate the retino-hypothalamic projection of the rat. Freshly isolated rat brains were dissected and a solution of cobaltous chloride was applied to one of the cut optic nerves. Sixteen hours later, after cobalt ions had passed into the brain along the entire length of the optic fibers, the preparation was treated with ammonium sulfide to precipitate the cobalt as cobalt sulfide. In thick light microscopic sections, cobalt-filled axons were visualized as black fibers against a light gold background. Such fibers were observed to leave the posterior medial portion of the optic chiasm and, after arching dorsally, to project into the posterior fifth of the suprachiasmatic nucleus (SCN), as well as into the rostral part of the arcuate nucleus. Neither bifurcation of these axons nor looping of the axons back to the chiasm was seen. Most fibers projected to the SCN contralateral to the filled nerve, but the projection represented less than 0.1 % of the total number of fibers in one optic nerve. These observations are considered to be graphic evidence of a retino-hypothalamic projection. The interpretation of the cobalt method is discussed, as are the functions of the connections that have been observed.
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  • 6
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    Cell & tissue research 170 (1976), S. 43-60 
    ISSN: 1432-0878
    Keywords: Interdigitating cells ; Lymph node ; Rat ; Ultrastructure ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is considered to be an argument in favour of the common origin of IDC's and macrophages. Ultrahistochemical studies with horseradish peroxidase (HRP) reveal no phagocytotic capacity of IDC's. After perfusion fixation containing ruthenium red (RR) the surface coat stains heavily: RR is also found deep in the membrane invaginations of the IDC, indicating the presence of polyanionic sialoglyco-proteins. The post-capillary-venules (PVC) are very permeable to both HRP and RR. The phosphotungstic acid-chromic acid stain (PTA-CrA) also reveals glycoproteins in the surface coat; these glycoproteins are susceptible to α-neuraminidase, whereas glycoproteins in the Golgi complexes, lysosomes and in the vesicular complexes of IDC are not. The glycoproteins of the latter are susceptible to 0.1 N NaOH. These findings indicate that IDC produce different kinds of glycoprotein, one of which may be secreted and act as a factor for stimulating peripheral T-lymphocytes. Intimate contact between IDC's and PCV's could be observed. It is therefore conceivable that IDC's play an important role in the homing of T-lymphocytes.
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  • 7
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Monoamine ; ZIO reaction ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The reaction of nerve endings in the median eminence of the rat to zinc iodide-osmium tetroxide (ZIO) staining was examined electron microscopically under normal and experimental conditions. The experimental condition of catecholamine exhaustion in the nerve endings was induced by the administration of H44/68 and reserpine. Vesicles in the terminals of catecholaminergic nerves reacted similarly to ZIO staining in both normal and experimental material. The majority of synaptic vesicles in various terminals gave a positive ZIO reaction. The neurosecretory elementary granules, however, failed to react with ZIO. On the other hand, some nerve terminals in the external layer of the median eminence showed a strong positive reaction in the cytoplasmic matrix, in mitochondria as well as in synaptic vesicles. These findings strongly suggest that the ZIO-positive substance in nerve terminals is not the transmitter itself, i.e. the monoamine, but rather represents a range of substances commonly found in various kinds of synaptic vesicles and is probably proteinaceous in nature. A brief discussion is also given on the difference in ZIO reactivity between neurosecretory elementary granules and small vesicles in the hypothalamo-hypophyseal tract.
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  • 8
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    Cell & tissue research 166 (1976), S. 407-412 
    ISSN: 1432-0878
    Keywords: Pituitary primordium ; Cytodifferentiation ; Rat ; Organ culture ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rathke's pouches isolated from rat fetuses on day 12 were maintained in organ culture for 9 days and investigated immunohistochemically to test whether or not the hypothalamus is involved in the cytodifferentiation of the adenohypophysis. The unlabeled antibody enzyme method demonstrated that the cultured tissue contains different types of glandular cells, i.e., adrenocorticotropin (ACTH)-, growth hormone (GH)-, luteinizing hormone (LH)-, thyrotropin (TSH)-, and prolactin-producing cells. Indirect evidence was also obtained to indicate the presence of melanocyte stimulating hormone (MSH)-cells. These findings suggest that adenohypophysial primordial cells of rats start to synthesize their respective hormones without stimuli from neurosecretory substances of the brain which are known to be essential for the maintenance of the secretory activity of the adult gland.
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  • 9
    ISSN: 1432-0878
    Keywords: Microtubules ; Tractus hypophyseus ; Diabetes insipidus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Evidence has already been adduced suggesting that an increase in microtubular number occurs in the tractus hypophyseus of rats stressed by the administration of hypertonic saline, and of rats with congenital diabetes insipidus (CDI). Since the tractus hypophyseus in these animals shows high secretory activity, it seems likely that the microtubular increase reflects the participation of microtubules in axoplasmic transport. To exclude, however, a congenital microtubular abnormality in CDI, affected newborn rats were examined. In these, the microtubular number was normal, thus suggesting that the increase in microtubular number seen in adult animals was not a congenital morphological abnormality. However, by 4 days of age there was a slight but statistically significant increase in microtubular number in affected rats, a change probably attributable to increased secretory activity.
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  • 10
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    Cell & tissue research 171 (1976), S. 375-379 
    ISSN: 1432-0878
    Keywords: Corpus luteum ; Autografts ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rat ovary has been transplanted successfully to subcutaneous tissue areas by several investigators. Light microscopy has revealed that corpora lutea in ovarian autografts are formed by luteinization of intact follicles and contain entrapped ova. In the present study, corpora lutea from autografted ovaries in castrate rats were obtained at metestrus and examined electron microscopically to determine whether their cellular morphology correlated with the normal progesterone levels in these animals. Cellular features usually accepted as regressive were apparent. The findings suggest either structural luteolysis is occurring before functional luteolysis or that the adrenal has increased steroidogenic activity in the castrate with ovarian autografts to account for the normal progesterone levels.
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  • 11
    ISSN: 1432-0878
    Keywords: Portal vein ; Rat ; Adrenergic innervation ; Postnatal ; Ontogenesis ; Histofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The postnatal development of the adrenergic innervation pattern in the rat portal vein has been studied with the histochemical fluorescence method of Hillarp and Falck. Stretch preparations and transverse freeze-dried sections of intact portal veins were studied from rats during the first 5 weeks of life and from adult rats. Orientation of undifferentiated smooth muscle cells into two layers was observed at 4 days of age. Dominance of the thick outer longitudinal muscle layer was apparent at two weeks of age. A terminal adrenergic nerve plexus with some varicosities was restricted outside the media at the end of the first week. Ingrowth of penetrating non-terminal adrenergic nerve fibers through the longitudinal muscle layer occurred during the second week of age when the main terminal nerve plexus was developing between the two muscle layers. After 3 weeks of age the adult pattern of a two-dimensional adrenergic plexus between the muscle was established. In the adult rat pharmacological treatment with nialamide and noradrenaline revealed the thin, penetrating non-terminal adrenergic nerve fibers in the longitudinal muscle layer which were poorly visible otherwise. The present observations strongly indicate that the main adrenergic plexus between the two muscle layers emanates directly from the outer axonal plexus. These findings are discussed regarding possible trophic interactions between ingrowing sympathetic adrenergic vasomotor nerves and maturing vascular smooth muscle.
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  • 12
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    Cell & tissue research 172 (1976), S. 195-203 
    ISSN: 1432-0878
    Keywords: Spleen ; Rat ; Marginal sinus ; Marginal zone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The marginal sinus in the spleen of the Wistar rat surrounds the follicle and has more numerous PAS positive fibers on the inner wall than on the outer wall. India ink- and lead oxide-gelatin were injected into the abdominal aorta. It was found that much of the india ink-gelatin accumulated in the marginal sinus, the marginal zone, and part of the red pulp, while most of the lead oxide-gelatin collected in the marginal sinus. Ultrastructurally, the capillaries of the follicle were found to open into the marginal sinus. Regions not perforated by the marginal sinus lie between the follicle and the marginal zone. The wall of the marginal sinus is discontinuous and the discontinuities are wider on the marginal zone side than on the follicle side. The relationship of these findings is discussed.
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  • 13
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    Cell & tissue research 173 (1976), S. 1-15 
    ISSN: 1432-0878
    Keywords: Carotid body ; Rat ; Innervation ; Three-dimensional reconstruction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the carotid body of adult rats was studied by means of serial ultrathin sections. A single branching nerve fiber innervates 12 chief cells through several kinds of terminals (vesicle-containing, mitochondrial sack, and calyx-type) in en-passant and bouton forms. Two types of synaptic contacts between nerve terminals and chief cells are found; type 1 in which chief cells are postsynaptic, and type 2 in which chief cells are presynaptic. Since a single nerve fiber (possibly from the glossopharyngeal nerve) forms both types of synapses with type 2 predominating, the nerve fiber is considered basically sensory or centripetal. In addition to their synaptic connections with sensory nerve fibers, chief cells located in the periphery of this organ are in synaptic relation with dendrites of a few ganglion cells adjacent to these cells. Here the chief cells are presynaptic. A few synaptic contacts between two adjacent chief cells are seen, and so are direct contacts between chief cells and preganglionic efferent nerve fibers terminating on ganglion cells.
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  • 14
    ISSN: 1432-0878
    Keywords: Exocytosis, endocytosis ; Freeze-etching ; Electron microscopy ; Membrane particles, rosettes ; Neurohypophysis, neurosecretion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Images have been obtained from freeze-etch replicas of neurohypophyses which are consistent with the view that orderly arranged aggregates of membrane particles occur in regions where fragments of membrane are being added to and taken away from the plasma membrane during secretion. Aggregates of particles included rosette-like and necklace-like patterns similar to those described by other authors at sites of exocytosis and endocytosis.
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  • 15
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    Cell & tissue research 165 (1976), S. 239-248 
    ISSN: 1432-0878
    Keywords: Superior cervical ganglion ; Rat ; Catecholamines ; Paraganglionic cells ; Monoamine synthesis inhibition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monoamine storage sites in paraganglionic (PG-)cells of the rat superior cervical ganglion were investigated by electron and fluorescence microscopy following treatment with p-chlorophenylalanine (pCPA), disulfiram or guanethidine respectively. Dense core vesicles in PG-cells are significantly decreased (p〈 0.001) in number following pCPA, and in the majority of these cells following disulfiram and guanethidine. However in a minor portion of PG-cells the latter agents cause an increase in number and in size of dense core vesicles, in parallel with structural alterations. In agreement with these electron microscopic findings fluorescence microscopic and cytophotometric evaluations reveal a general decrease in catecholamine content with few cells showing an increase. The findings provide a morphological basis for the assumption, that monoamine storage sites in PG-cells can be decreased by inhibition of monoamine synthesis, following administration of pCPA, disulfiram and guanethidine. However the two types of responses of PG-cells which occur after disulfiram and guanethidine demonstrate a functional heterogeneity of this cell system in the rat superior cervical ganglion which is discussed.
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  • 16
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    Cell & tissue research 166 (1976), S. 135-143 
    ISSN: 1432-0878
    Keywords: Synaptic ribbons ; Pineal organ ; Rat ; Ultracytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The synaptic complexes of the rat pinealocytes are neither cholinergic nor adrenergic. In the synaptic vesicles, a neurotransmitter carrier substance of lipid nature reacting with OsO4-Zn I2 mixture (similar to that present in both cholinergic and adrenergic vesicles) was not found. In addition, there were no indications of glucose-6-phosphatase or thiamine-pyrophosphatase activity in the synaptic vesicles. Thus, it appears that the synaptic vesicles do not originate from the rough or smooth endoplasmic reticulum. The synaptic ribbons do not contain carbohydrates, are of protein nature and possess some chemical resemblance to microtubules and microtubular bouquets. Appropriate ultracytochemical reactions have not shown detectable quantities of sodium and calcium ions in pinealocyte synaptic complexes.
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  • 17
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    Cell & tissue research 168 (1976), S. 411-418 
    ISSN: 1432-0878
    Keywords: Luteinizing hormone-releasing hormone ; Hypothalamus ; Rat ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of radioactivity in the rat hypothalamus after intraventricular injection of tritiated luteinizing hormone-releasing hormone (LH-RH) was studied by autoradiography and compared with that expected from Fick's second law which defines the character of ordinary diffusion. The results suggest that LH-RH penetrates through the ependyma and thin subependymal layer in the median eminence and into the suprachiasmatic area by ordinary diffusion. By contrast, concentration of radioactivity in outer layers of the median eminence is increased and, therefore, can not be explained by the diffusion law. In this region incorporation of radioactivity into cell bodies was demonstrated. In the subependymal layer of the median eminence a few rows of silver grains seemingly located in cellular processes were also observed. The diffusion coefficient in the periventricular zone was lower than that in the deeper tissue. It is suggested that the ependymal-subependymal complex of the third and lateral ventricles acts as a passive membrane towards LH-RH contained in the cerebrospinal fluid. The significance of this mechanism for the intrahypothalamic transport of RHs is discussed.
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  • 18
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    Cell & tissue research 168 (1976), S. 549-559 
    ISSN: 1432-0878
    Keywords: Hypothalamic-hypophysial-adrenal axis ; Rat ; Development ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenal glands of decapitated and encephalectomized fetal rats were investigated electron microscopically and compared to those of normal intact fetal rats. Although the adrenal cortices did not show three zones (zona glomerulosa, fasciculata, and reticularis) on the 16.5th day of gestation when the decapitation or encephalectomy was carried out in utero, the zonation was recognized in fetuses operated on the 21.5th day of gestation. The same was true for normal control fetuses. However, cytoplasmic characteristics suggesting steroidogenesis in the cortical cells were reduced to various degrees in the encephalectomized or decapitated fetuses, especially in the latter ones. The change in cytoplasmic appearance was more conspicuous in the inner portion of the cortex. This result suggests that for the maintenance of normal adrenocortical function the hypothalamus may be indispensable even during the prenatal life of rats.
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  • 19
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    Fresenius' Zeitschrift für analytische Chemie 279 (1976), S. 208-208 
    ISSN: 1618-2650
    Keywords: Best. von Palladium mit 2-Chlorphenothiazin ; Spektralphotometrie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 20
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    Fresenius' Zeitschrift für analytische Chemie 279 (1976), S. 364-364 
    ISSN: 1618-2650
    Keywords: Best. von Titan(IV) ; Spektralphotometrie ; Extraktion als Thiocyanat mit Mesityloxid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 21
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    Fresenius' Zeitschrift für analytische Chemie 278 (1976), S. 127-127 
    ISSN: 1618-2650
    Keywords: Best. von Kobalt mit 6-Nitrochinoxalin-2,3-dithiol ; Spektralphotometrie ; Spuren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 22
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    Fresenius' Zeitschrift für analytische Chemie 278 (1976), S. 285-286 
    ISSN: 1618-2650
    Keywords: Best. von Kupfer mit 4-(2-Pyridylazo)-resorcin ; Spektralphotometrie ; Untersuchung der Komplexe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 23
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    Fresenius' Zeitschrift für analytische Chemie 278 (1976), S. 364-364 
    ISSN: 1618-2650
    Keywords: Best. von Nickel mit Äthylxanthat ; Spektralphotometrie
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    Topics: Chemistry and Pharmacology
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  • 24
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    Cell & tissue research 173 (1976), S. 179-191 
    ISSN: 1432-0878
    Keywords: Epiglottis ; Rat ; Elastic cartilage ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Chondrocytes of the rat epiglottis contain large amounts of glycogen and lipids, which often make the cells resemble fat cells. The content of lipids is interpreted as being related to the function of the cells. The membranes of some of the large vacuoles are stained with ruthenium red. The cells give rise to long cytoplasmic processes. As in hyaline cartilage the intercellular substance consists of a fine network containing proteoglycan granules together with thicker cross striated fibers. Furthermore elastic fibers are found, consisting of amorphous and microfibrillar parts. In the matrix, both lysosome-like granules and more or less empty vesicles are observed. Accumulations of a finely particulate electron dense material and of a translucent amorphous material containing membrane bound granules are found in some lacunae situated in the outer part of the cartilage. These accumulations are possibly related to the development of collagenous and elastic fibers.
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  • 25
    ISSN: 1432-0878
    Keywords: Liver ; Rat ; Sinusoid ; Pit cell ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pit cells — a new type of cell first described here and so named because they contain highly characteristic granules — are situated in the wall of rat liver sinusoids, and have hyaloplasmic pseudopodia intermingling with the microvilli of the parenchymal cells. The characteristic granules are mainly situated at one side of the nucleus, the other side showing organelle-free hyaloplasm. Pit cells are also found in portal tracts and in granuloma-like cellular aggregates. They also occur in rat peripheral blood, although there are morphological differences between cells in these two sites. Pit cells can be regarded as regular inhabitants of the sinusoidal wall, and therefore belong to the series of sinusoidal cells, i.e., the endothelial (Wisse, 1972), Kupffer (Widmann et al., 1972; Wisse and Daems, 1970; Wisse, 1974a,b), and fat-storing cells (Ito, 1973). Pit cells do not phagocytose and do not react to a great number of experimental conditions, to which endothelial and Kupffer cells do react (Wisse, 1972, 1974b). Mitosis has been observed in a pit cell. The function of pit cells remains obscure, but an endocrine function is suggested by the morphology of their highly characteristic granules.
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  • 26
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    Cell & tissue research 173 (1976), S. 543-554 
    ISSN: 1432-0878
    Keywords: Prostate ; Rat ; Dog ; Basal cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the basal cells of rat lateral and ventral prostate and of dog prostate has been studied. Basal cells from both species appear as undifferentiated cells, characterised by a lack of cytoplasmic organelles and a poorly developed Golgi complex and endoplasmic reticulum. The presence of cytoplasmic filaments and micropinocytosis is not considered to be sufficient evidence to assume any similarity to myoepithelium, as has been previously suggested. Basal cells are instead considered to be precursors of secretory epithelial cells.
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  • 27
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    Cell & tissue research 174 (1976), S. 83-97 
    ISSN: 1432-0878
    Keywords: 6-hydroxydopamine ; Extraneuronal effects ; Rat ; Adrenal ; Tissue culture ; Electron microscopy ; Microspectrofluorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of various concentrations of 6-hydroxydopamine (6OHDA) on rat adrenocortical cells in tissue culture were studied with phase contrast and electron microscopy. With 40 mg/l of 6-OHDA the first signs of alteration as revealed by microcinematography appeared in isolated cortical cells as early as 15 min after addition of the drug. There was a cessation of movement of cell organelles and an immobilisation of membrane undulations followed by the development of dark inclusion bodies. The cells underwent increasing shrinkage and collapsed by 11/2 h. Chromaffin cells were not affected until 45 min after exposure to the drug and neurons were the most resistant population. However 61/2 h after application of the drug most cells in the culture were dead. 6-OHDA applied in different doses and to adrenal expiants did not alter the sequence of events. Ultrastructurally cortex cells underwent damage along two lines: they either showed lytic changes or developed various types of dense bodies before reaching the lytic stage. Treatment of cortical cells with 40 mg/l 5-or 6-OHDA followed by exposure to buffered 2% glyoxylic acid and heat did not produce a fluorescence within the cells. Microspectrofluorimetry on amine models with noradrenaline, 5- and 6-OHDA revealed that neither 5-nor 6-OHDA are capable to form a fluorophore with glyoxylic acid.
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    ISSN: 1432-0878
    Keywords: Ovarian autografts ; Autonomic innervation ; Ovarian steroids ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ovaries were removed from female rats and immediately autografted into a subcutaneous pouch in the flank in order to quantitate the relationship of graft re-innervation, steroid secretion and vaginal smear pattern. Animals were killed at three time periods: three days after grafting, on the first day a cornified vaginal smear appeared and at the first metestrus. In addition, control animals were killed at metestrus. Plasma samples were obtained from all rats and analyzed for estradiol-17β and progesterone concentration by radioimmunoassay. At the first day of vaginal cornification after grafting, plasma estradiol-17β (45.8±4.0 pg/ml) was elevated in comparison to controls at metestrus (24.0±2.6 pg/ml), but plasma progesterone (21.5±4.0 ng/ml) was not different (30.6±1.7 ng/ml). Subsequently, at the first metestrus following grafting, plasma estradiol-17β (23.0±3.5 pg/ml) was comparable to control values. In contrast, progesterone was decreased (17.5±1.9 ng/ml). A definite correlation was detected between the vaginal smear and plasma levels of steroid hormones in the castrated female rat with subcutaneous ovarian autografts. Histochemical techniques were used to study the adrenergic and cholinergic innervations of grafts three days after grafting, at the first day of vaginal cornification, and at the first metestrus. No correlation was shown between density of adrenergic or cholinergic innervation and plasma levels of estradiol-17β and progesterone or onset of a cycling vaginal smear.
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    Cell & tissue research 172 (1976), S. 133-144 
    ISSN: 1432-0878
    Keywords: Cornea ; Nerves ; Rat ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the corneal nerves of the rat was studied in tissue fixed by immersion in and by perfusion with glutaraldehyde-containing fixatives. Of the four types of axonal terminal identified in the nerves, those with the features of adrenergic and cholinergic terminals were confined to the nerves at the limbus and were concentrated in the perivascular plexuses. The remaining two types of terminal were found on axons located in all parts of the cornea and on both intraepithelial axons and axons in the stromal nerves. Of these, one contained the numerous mitochondria which occur in the terminals of axons associated with known mechanoreceptors and the second contained variable and often small numbers of both clear and large dense-cored vesicles. While most of the mitochondria-containing terminals were seen in nerves located near the periphery, vesicle-containing terminals were numerous in all of the nerves, and especially in those in the avascular cornea. In material fixed by immersion in glutaraldehyde-paraformaldehyde, the vesicle-containing terminals appeared to be dilated, but in material fixed by perfusion there was little evidence of any increase in the diameter of the axons in the terminal regions. The structure of the terminals was compared with that of the terminals of axons identified in the nerves of the skin and the urinary tract and the differences in the vesicle content of the terminals to those reported in other studies of the corneal nerves was related to the use of different fixation procedures. The possibility that axons possessing such terminals are identical with the beaded axons and both the cholinesterase-positive and fluorescent axons demonstrated in light microscopical studies of the corneal nerves is discussed, and the widespread distribution of the axons in the cornea is equated with the hypothesis that they are afferent in nature and represent the peripheral receptors for pain impulses.
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    Fresenius' Zeitschrift für analytische Chemie 281 (1976), S. 143-143 
    ISSN: 1618-2650
    Keywords: Best. von Platin(IV) mit Thiobenzhydrazid ; Spektralphotometrie ; Extraktion
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    Topics: Chemistry and Pharmacology
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    Fresenius' Zeitschrift für analytische Chemie 281 (1976), S. 384-384 
    ISSN: 1618-2650
    Keywords: Best. von Desoxyribonucleinsäure, Ribonucleinsäure, Protein ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 31-35 
    ISSN: 1618-2650
    Keywords: Spektralphotometrie ; Transformation Gaußscher Absorptionskurven
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The convolution of Gaussian absorption curves using orthogonal polynomials has been studied. An absorption curve can usually be considered as a sum of Gaussian curves. The p2-convoluted log A versus λ curve is independent of concentration and path-length and the optimum coefficient can be used as an index of purity for absorbing compounds.
    Notes: Zusammenfassung Die Transformation von Gaußschen Absorptionskurven unter Verwendung von Orthogonalpolynomen wurde untersucht, denn in der Regel kann eine Absorptionskurve als Summe von Gauß-Kurven betrachtet werden. Der quadratische Polynomkoeffizient aus log A gegen λ ist unabhängig von Konzentration und Schichtdicke und kann als Reinheitsindex benutzt werden.
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 47-47 
    ISSN: 1618-2650
    Keywords: Best. von Calcium, Magnesium mit SPADNS ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 141-141 
    ISSN: 1618-2650
    Keywords: Best. von Mangan(II) mit Kakothelin ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 220-220 
    ISSN: 1618-2650
    Keywords: Best. von Eisen mit Thiocyanat und Monooctyl-α-anilinobenzylphosphonat ; Spektralphotometrie ; Mischkomplex
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 222-222 
    ISSN: 1618-2650
    Keywords: Best. von Eisen(II), Kobalt(II) mit 7-Nitroso-8-hydroxychinolin-5-sulfonsäure ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 278 (1976), S. 367-367 
    ISSN: 1618-2650
    Keywords: Best. von Polynitroverbindungen mit Borhydrid ; Spektralphotometrie ; aromatische m-Polynitroverbindungen
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    Fresenius' Zeitschrift für analytische Chemie 279 (1976), S. 167-168 
    ISSN: 1618-2650
    Keywords: Best. von Sauerstoff, Partialdruck ; Spektralphotometrie ; org. Kobaltchelate
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    Fresenius' Zeitschrift für analytische Chemie 279 (1976), S. 208-208 
    ISSN: 1618-2650
    Keywords: Best. von Methylenblau ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 279 (1976), S. 207-207 
    ISSN: 1618-2650
    Keywords: Best. von Palladium neben Platin mit α-Benzoinoxim ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 280 (1976), S. 29-29 
    ISSN: 1618-2650
    Keywords: Best. von Platin(IV) mit Dithiooxamid ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 280 (1976), S. 21-23 
    ISSN: 1618-2650
    Keywords: Best. von Phosphat über Molybdänblau ; Spektralphotometrie ; Optimierung des Verfahrens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary This article discusses the optimization of a phosphate analysis via molybdenum blue, using both factor analysis and the simplex technique. As a result of this study, a final recommended procedure for the photometric determination of phosphate has been given.
    Notes: Zusammenfassung Zur Optimierung einer photometrischen Methode zur Phosphatbestimmung werden die genannten Methoden diskutiert und als Ergebnis der Untersuchung eine endgültige Arbeitsweise vorgeschlagen.
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    Fresenius' Zeitschrift für analytische Chemie 280 (1976), S. 30-30 
    ISSN: 1618-2650
    Keywords: Best. von Titan(IV) mit Benzhydroxamsäure ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 281 (1976), S. 141-141 
    ISSN: 1618-2650
    Keywords: Best. von Jod in Pflanzenmaterial ; Spektralphotometrie ; Sandell-Kolthoff-Reaktion, Langzeitversuche
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    Fresenius' Zeitschrift für analytische Chemie 281 (1976), S. 361-364 
    ISSN: 1618-2650
    Keywords: Best. von Eisen(III) mit Chinaldinsäure-N-oxid ; Spektralphotometrie ; Extraktion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A simple and sensitive method for the determination of iron(III) is described. It is based on the extraction of the yellow-coloured complex of iron with quinaldic acid-N-oxide in chloroform. The absorption maximum is at 380 nm with molar absorptivity ε 380=1.03 · 104. Beer's Law is obeyed over the range 0.6–6 ppm iron with relative standard deviation of +-0.3% (Sandell sensitivity 0.005 μg/ml/cm2). The absorbance of the reagent blank is however high with consequent occasional disturbances, which are avoided when measured at 395 nm; molar absorptivity ε 395=7.4 · 103; effective concentration range 3–8 ppm iron with relative standard deviation +-0.5% (Sandell sensitivity 0.008μg/ml/cm2). A fairly large number of common ions do not interfere. Job's method of continuous variations and Asmus method indicate a iron-reagent ratio of 1∶3. Heterogeneous formation constant is K D =2 · 108.
    Notes: Zusammenfassung Die einfache und empfindliche Bestimmung beruht auf der Extraktion des gelben Komplexes von Eisen(III) mit Chinaldinsäure-N-oxid in Chloroform. Das Absorptionsmaximum liegt bei 380 nm mit dem molaren Extinktionskoeffizient ε 380=1.03 · 104. Das Beersche Gesetz gilt im Bereich 0,6–6 ppm Eisen. Die relative Standardabweichung beträgt+-0,3% (Sandell-Empfindlichkeit 0,005μg/ml/cm2). Die Absorption der Blindprobe ist jedoch hoch und verursacht manchmal Störungen, die beim Messen bei 395 nm vermieden werden; molarer Extinktionskoeffizient ε 395= 7,4 · 103; im Konzentrationsbereich 3–8 ppm Eisen beträgt die relative Standardabweichung+-0,5% (Sandell-Empfindlichkeit 0,008μg/ml/cm2). Eine ziemlich große Zahl von Fremdionen bewirkt keine Störung. Methoden nach Job und Asmus liefern ein Verhältnis Eisen/Reagens wie 1∶3. Die heterogene Bildungskonstante beträgt K D =2 · 108.
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    Fresenius' Zeitschrift für analytische Chemie 281 (1976), S. 371-377 
    ISSN: 1618-2650
    Keywords: Best. von Östrogenen, Androgenen, Sexualhormonen ; Spektralphotometrie ; Reaktion mit Schwefelsäure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A detailed spectrophotometric study of the reaction of estrogens and androgens with 98% sulphuric acid at 20° C and of the effect of various dilutions with water on the nature of the spectra is presented. Dilution of the androgens-acid and estrogens-acid solutions with water to 40% and 80% v/v acid, respectively, increases the absorption intensity 3 to 11 times. The optimum conditions for determination of down to 2μg/ml of estrone, estradiol, ethinylestradiol, testosterone, and rostanalone and progesterone are reported. The results show a relative standard deviation of +-1.5 %.
    Notes: Zusammenfassung Die Reaktion der Östrogene und Androgene mit 98%iger Schwefelsäure bei 20° C und der Einfluß von Verdünnungen. mit Wasser auf die Art der Spektren wurden eingehend untersucht. Verdünnungen auf einen Säuregehalt von 40% und 80% bewirken eine 3–11fache Zunahme der Absorption. Die optimalen Bedingungen zur Bestimmung von 〉2μg/ml Östron, Östradiol, Äthinylöstradiol, Testosteron, Androstanalon und Progesteron wurden ausgearbeitet. Die relative Standardabweichung beträgt+-1,5%.
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 48-48 
    ISSN: 1618-2650
    Keywords: Best. von Thallium(I) ; Spektralphotometrie ; indirekt mit Lucigenin
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 140-140 
    ISSN: 1618-2650
    Keywords: Best. von Vanadat, Chromat, Wolframat mit Triphenylmethanfarbstoffen ; Spektralphotometrie ; Kristallviolett, Malachitgrün, Fuchsin
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 141-141 
    ISSN: 1618-2650
    Keywords: Best. von Osmium(VIII) mit Trifluoperazin ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 143-143 
    ISSN: 1618-2650
    Keywords: Best. von Äthylenglykol in Propylenglykol ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 218-218 
    ISSN: 1618-2650
    Keywords: Best. von Kupfer mit Tetraäthylenpentamin oder Triäthylentetramin ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 221-221 
    ISSN: 1618-2650
    Keywords: Best, von Eisen(II), Kobalt(II) mit 2,4-Dihydroxy-3-nitrosopyridin ; Spektralphotometrie
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 462-462 
    ISSN: 1618-2650
    Keywords: Bestimmung von Vanadium ; Extraktion ; Spektralphotometrie ; Elektrolysegifte
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    Fresenius' Zeitschrift für analytische Chemie 282 (1976), S. 460-461 
    ISSN: 1618-2650
    Keywords: Best. von Phosphor in Kupferlegierungen ; Spektralphotometrie ; Sn-haltige Cu-Legierungen
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