ALBERT

Notes: A thin, compressible, lateral suture and ventral plate overlap permit limited movement of the thick and rigid dorsal and ventral plates of Fuscouropoda agitans. Seven pairs of large dermal glands debouch onto the surface. Trochanteral rotation permits defensive leg adpresion and an insectan type of ambulation. The complex hypopharynx-pedipalpal-coxae has a buccal and cheliceral cavity separated by an atriculated epipharynx. The pharynx is Y-shaped in cross section. Extensive paired salivary glands lie above the very long and dexterous 3-segmented chelicerae, and a large pair of coxal glands debouch on coxae 1. From four blunt-ended tracheae, bundles of unbranching tracheoles extend in specific tracts to all organs. The ventriculus is small with three pairs of large caeca; a tightly packed single layer of digestive cells individually enlarged to absorb-phagocytize and digest the food. A typical mesostigmatid excretory tube is present. A typical acarine synganglion is present; mixed nerves have a basal swelling. A postulated neurosecretory organ arises from the pedipalpal nerve. The oocytes enlarge within funicular stalks from the walls of the small median ovary. A large spermatophore is stored in the seminal vesicle; fertilization occurs during oviposition. A tension hinge partially opens both male and female genital plates; closure effected by muscles acting on very long genital plate apodemes. Within sequentially produced spermatogonial cysts of the testes, meiosis is completely synchronous. A large, multilobed male accessory gland produces a large volume of seminal fluid; a mixture of at least four secretions. The origins and msertions of the body wall, genital organ, digestive tract, mouthpart and leg muscles are listed and illustrated. A comparison of anactinotrichid and actinotrichid mites indicates fundamental and consistent morphological differences in aspects of the cuticle, leg articulations, digestive system, excretory system, reproductive system and coxal glands.

Notes: An investigation of the structure of the iridescent scales of the green hairstreak, Callophrys rubi, reveals an internal lattice which is probably cubic close-packed in form. We present a model which explains the formation of the lattice in terms of packing of spheres and surface tension forces and generalize these results to internal structures in other Lepidopteran scales.

Notes: There are 36 to 42 taste bristles on each half of the labellum of Drosophila melanogaster; most of them are two-pronged with a pouch between them. Some end bluntly with a pore at the tip.Each taste-bristle has two lumina: one is circular, the other crescent-like in cross section. In most bristles four dendrites of chemoreceptor neurons run along the circular lumen. In five to seven taste-bristles only two chemoreceptor neurons are found. A mechanoreceptor neuron sends a dendrite to the base of each taste-bristle.The dendrites are surrounded by four concentrically-arranged sheath cells. The inner cell secretes the cuticular sheath; cells II and III are presumably two trichogens, one secreting the bristle material around the circular lumen, the other around the crescent-like lumen. Cell IV, especially rich in bundles of microtubules, secretes the cuticle of the socket, and corresponds to the tormogen. The neurons have the typical structure found in insect sensilla. In many sensilla one neuron is less electron-dense than the others and may be the water-sensor.On the medial side of the labellum between the pseudotracheae are rows of taste pegs covered by folds. In each peg one chemoreceptor and one mechanoreceptor are found.The number of axons in each labial nerve agrees with the total number of dendrites in all taste organs of each lobe.

Notes: Stichopus moebii, a sea cucumber, has a closed circulatory system which is unique in its degree of development for the phylum Echinodermata. The gross anatomy, histology and fine structure of the system were studied. Blood vessels consist of a coelomic surface of ciliated epithelium, a layer of muscle and nerve cells, followed by connective tissue and luminal lining of endothelium. Basically the blood vascular system consists of two major vessels running parallel to the gut: the dorsal vessel pumps colorless blood via the vessels within the walls of the intestine into the ventral vessel. There are two specialized areas of the circulation: (1) At the upper small intestine 120 to 150 muscular single-chambered hearts pump blood from the dorsal vessel into a series of intestinal plates. (2) At the lower region of the small intestine the vasculature is associated with the left respiratory tree. Blood passing from the dorsal pulmonary vessel can take two routes to the gut, it either passes through myriads of minute respiratory shunt vessels entangled with the respiratory tree or it passes through a unique follicle network consisting of tiny channels periodically dilated into chambers filled with iron deposits, necrotic cells and developing coelomocytes.

Notes: Anatomical components of afferent innervation in the rim of the octopus sucker are described. In the sensory epithelium under the smooth cuticle two associated ciliated receptor cell-types (presumably chemosensitive) occur in clusters. A third ciliated receptor cell-type under the toothed cuticle may be a mechanoreceptor. A non-ciliated receptor cell-type of unknown function, under the toothed cuticle, is characterized by a microvillus-lined apical canal containing dense granular material. The axons of the latter two receptors go directly into large nerve tracts which nm through the infundibular muscle and on to the ganglion of the sucker. The axons of the first cell-types terminate on interneurons either in the base of the epithelium or below the epithelium. All the interneurons of the basal region of the epithelium migrate centripetally and develop into encapsulated interneurons. Within the epithelium, fine fibers provide collateral contact among cluster receptors. Collateral interaction among basal and encapsulated interneurons occur in the infundibular plexus. The microanatomy of the rim of the sucker suggests that chemosensory cues are funneled into the interneurons where they are concentrated into integrated signals, while other sensory input is probably sent directly to the ganglia of the sucker and/or arm.

Notes: The pyloric region of Eosentomon and Acerentomon (Insecta, Protura) is described. In both species the posterior cells of the midgut carry short microvilli. Beneath the epithelial cells there is a muscular pyloric sphincter for closing the intestinal lumen. Behind the sphincter is a wide pyloric chamber lined by cells with very long microvilli which point anteriorly toward the midgut. These cells regulate the passage of the intestinal contents into the hindgut. Secretions from the Malpighian papillae are emitted into the gut at this level. In Eosentomon three regions (R1, R2 and R3) are visible in the Malpighian papillae, whereas in Acerentomon region R1 is lacking. The R1 region contains secretory cells with elaborate glycoprotein-containing granules. The R2 region is composed of cells somewhat resembling the secretory cells of Malpighian tubules of insects. Presumably R1 and R2 cells emit secretions into the central cavity of each papilla. Cells of R3 form a duct for the secretion. It is suggested that the R2 region represents a basic excretory region, common to Protura, whereas the R1 region, in Eosentomon, may be a specialized area performing supplementary excretory functions.

Notes: This manuscript describes in precise detail the ultrastructural alterations produced as a result of laser microirradiation of nucleoli and nucleoplasm of tissue culture cells. Because of the general difficulty of single cell recovery, flat embedding, and serial sectioning, very few studies have ever been conducted on microbeam irradiated cells; yet the use of the microbeam technique has become widespread in functional studies of the nucleus. The results presented here demonstrate two classes of lesion material: small spherical electron dense bodies 0.05-0.02 μm in diameter and a larger, more irregular electron dense material up to 1 μm in length. The occurrence of these different types of lesion materials is described in control irradiated nucleoli and nucleoplasm, irradiated nucleoli and nucleoplasm in quinacrine treated cells, irradiated nucleoli and nucleoplasm in actinomycin D treated cells, and irradiated nucleoli and nucleoplasm in combined actinomycin D and quinacrine treated cells. In all the cells in which actinomycin D was employed, nucleoli were selectively irradiated in either their granular or fibrillar zones. The results of the ultrastructural studies are discussed in light of earlier functional studies.

Notes: The general form and adaptation of the digastric muscle in carnivores are reviewed and discussed. The digastric muscle differs from the general plan in certain aquatic carnivores and felids. In the pertinent aquatic species the muscle is enlarged. The observations suggest that the enlargement is an adaptation for rapidly opening the jaws against the resistance of water. In felids, the insertion of the muscle is much farther forward than in most other carnivores. The observations suggest that the development of short jaws in felids necessitated a compensatory anterior relocation of the digastric insertion in order to preserve the ability to achieve a large gape.

Notes: Homolid crabs (Hypsophrys) from water deeper than 700 m in the Straits of Florida and Arabian Sea have smooth darkened oval spots contrasting with the surrounding roughened integument on inner and outer surfaces of each pincer at the base of the fixed finger. Cuticle is thinner over these spots than over surrounaing tissues. Beneath each spot is an organ composed of two markedly contrasting layers of tissue: (1) an outer, densely staining layer of tightly packed tubules, relatively straight and perpendicular to the overlying surface proximally but progressively convoluted and narrowed distally, finally ending blindly in association with the overlying thinned cuticle; (2) an inner layer of relatively large, eosinophilic, irregular cells with dark nuclei also trending at a right angle to the integument and bulging into the hemal sinus of the hand but separated from it by an epidermal lining. Droplets secreted from the inner layer apparently move into and along the tubules. Similar organs are known in no other crabs. The function is unknown but the structure suggests that they may be photophores.

Notes: The ultrastructure of a well studied insect chemosensory unit is presented in this report. Two separate lumina are present in this chemosensory unit, the trichogen and sensillar lumina. The fluid within the trichogen lumen exclusively bathes the dendritic terminals, and may be involved with the reception and/or modulation of environmental stimuli. Cytoplasmic extensions of the trichogen cell which line the trichogen lumen may be involved in the production of the cuticular sheath. The sensillar lumen is bordered by the tormogen and a sleeve cell, and is continuous with the unoccupied channel of the setal shaft. Functions for the various cellular components of the blowfly chemoreceptor sensillum are offered.

Notes: This study indicates that eggs containing calcium carbonate crystals occur in at least 36 of the 65 known families of the land snails (class Gastropoda: order Stylommatophora). Eggs from 22 of these families were available for examination. The x-ray diffraction data, available for the first time for 21 of these families, shows that these egg shells are all made of calcite only, or of a combination of calcite with smaller amounts of aragonite. All of the snail (body) shells examined were made of aragonite only. This is the first ultrastructural investigation of these egg shells, and it indicates that the eggs exhibit enough structural diversity to allow identification of parental animals to genus, and often to species level solely on the basis of egg shell ultrastructure.All of the calcified eggs may be divided into two groups: (1) partly calcified, with discrete crystals of CaCo3 dispersed in the jelly layer, and (2) heavily calcified, with a hard, brittle egg shell made of fused crystals of CaCO3 much like an avian egg. Both types of calcified eggs occur in oviparous as well as in ovoviviparous snails. Because of the wide distribution of calcified eggs in the Stylommatophora, and because of the occurrence of heavily calcified eggs in ancient families such as Partulidae, Endodontidae, and Zonitidae, the calcified egg is viewed as a primitive land snail trait associated with terrestrial adaptation. The function of the calcified egg shell, in addition to mechanical support of egg contents, is to supply the developing embryo with enough calcium to form the embryonic shell by the time of hatching.

Notes: Projection microradiography was used to determine the density and orientation of the force transmitting structures, i.e., trabeculae and bone lying between approximately parallel vascular canals, within the bones of cat skulls. The organisation in the skulls was confirmed statistically for a total of ten cats. The results of the observations showed that within specific areas of the skull a high degree of structural orientation and an increased density of osseous structures was present. The distribution of these characters corresponded in contiguous bones such that a continuum of structural organisation was established between the alveolar region and the site of attachment of the temporalis and masseter muscles and the glenoid region.The patterns of force transmission during jaw closure were determined when a resistance was placed initially between the canines and then the carnassials. An analysis was first carried out on dry skulls using colophonium resin to determine the direction of the force distribution. The nature and the approximate magnitude of the forces were ascertained by replacing the resin with strain gauges. The basic similarities in the strain patterns recorded from the dry skulls and those from the ten anaesthetised cats in which strain gauges had been intra-vitally implanted, substantiated the recordings made on the dry skulls. Combination of the results from the three sets of experiments defined the patterns of force distribution in the cat skull during the closure of the mandible against a resistance. The results showed that: (1) the combined action of the temporalis and masseter muscles tended to reduce the overall strain in the skull bones, and that the deformations produced by the action of the masseter were greater than that exerted by the temporalis muscles; (2) during biting, whether the resistance was placed between the canines or carnassials, compressive forces predominated in the facial bones; (3) small movements observed between facial bones indicated the presence of a flexible component within the skull, thus allowing large forces to be exerted during biting without overstressing the facial bones; (4) the glenoid fossa is part of a force bearing joint; (5) forces generated during biting were resisted within the skull by forces of an opposite nature generated within the system, the incompressible nature of bone and by the effect of the soft tissues; (6) the nature and the magnitude of the strain altered when a resistance was placed at the canines and then at the carnassials; however, the pattern of force distribution within the skull remained the same; (7) there was a direct correspondence between the detailed structural organisation of the bones and the patterns of force distribution. This conclusion would appear to apply in general to mammalian skulls. The study also emphasises the importance, neglected hitherto, of carrying out a variety of experiments to determine the patterns of force distribution in bones.The Trajectorial Theory of bone organisation is discussed and, on the basis of the results obtained, a modified theory is proposed. This states that: the structural continuum is common to the compact and cancellous bone and comprises bony bars which are aligned in the optimum direction for the transmission of force to a region in the bone or bones where it is effectively resisted.

Notes: The tarsi of all three pairs of legs of both sexes of Aedes aegypti (L.) bear spine sensilla, five types of hair sensilla, which are designated A, B, C1, C2 and C3, and campaniform sensilla. Type A and B hairs, spines, and cam-paniform sensilla are innervated by one neuron with a tubular body, a characteristic of cuticular mechanoreceptors. In particular the hairs and spines are tactile receptors and the campaniform sensilla are proprioceptors. The C1, C2, and C3 hair sensilla have the morphological features of contact chemoreceptors. Type C1 and C3 hairs are innervated by five and four neurons, respectively, which extend to the tip of the hair. Type C2 is innervated by five neurons, one of which terminates at the base of the hair in a tubular body while the remaining four extend to the tip of the hair. The role of the type C hairs in oviposition behavior, nectar feeding, and recognition of conspecific females is discussed. Presumed efferent neurosecretory fibers occur near the spine and hair sensilla.

Notes: The micro-anatomy of the corpuscles of Stannius of the toadfish, Opsanus tau, an aglomerular marine teleost, has been studied by light and electron microscopy. The corpuscles are composed of extensively anastomosed cords of epithelial cells which maintain intimate contact with blood capillaries. Most of the epithelial cells contain acidophilic granules which also show a positive reaction with the periodic acid-Schiff technique and aldehyde fuchsin. On the basis of fine structural criteria, three cell types can be recognized. The granular cells contain abundant quantities of granular endoplasmic reticulum, ribosomes, Golgi apparatus with prosecretory granules, coated vesicles, polymorphic mitochondria with lamellar cristae, filaments, microtubules, a cilium, a variety of lysosome-like dense bodies, glycogen particles, lipid droplets, secretory granules and intranuclear lipid-like inclusions. One variety of agranular cell (type I) is characterized by the total absence of secretory granules, but it contains large amounts of granular endoplasmic reticulum and ribosomes, conspicuous profiles of Golgi apparatus, coated vesicles and sometimes an abundance of glycogen. Another variety of agranular cell (type II) has poorly developed cytoplasmic organelles. The perivascular space between the capillary and parenchyma contains connective tissue cells and abundant nerve fibers. The different types of epithelial cells observed in the corpuscles of Stannius of this fish may represent functional stages of the secretory cycle in a single cell type.

Notes: The nervous system of the maldanid polychaetes Clymenella torquata (Leidy) and Euclymene oerstedi (Claparede) (= Caesicirrus neglectus [Arwidsson, '11-'12]) retains its primitive association with the epidermis. It shows only slight metamerism in the presence of larger collections of neurones opposite the parapodia and of larger nerves at the segmental boundaries. Multicellular giant fibers are present in the ventral nerve cord; giant neurones which show a characteristic pattern of distribution in each species are also present. The cerebral ganglia supply nerves to the prostomial wall, nuchal grooves and the wall of the buccal cavity, and a pair of large nerves from the circumpharyngeal connectives also appear to join the buccal system. The organs of special sense are the elongated prostomial nuchal grooves, and prostomial ocelli in Euclymene but not in Clymenella. Statocysts are absent.Four pairs of nephromixia are present. They lie in the aseptate anterior trunk, in chaetigers 5-9 of Clymenella, and 6-10 of Euclymene. The nephridiopores lie at the ventral ends of the neuropodia of chaetigers 6-9 and 7-10, respectively. Each nephromixium consists of coelomostome, tubule and contractile bladder. The wall of the tubule and bladder consists of both excretory and ciliated cells. Most of the cytoplasm of the latter forms a bounding layer at the outer surface. The cytoplasm of the excretory cells contains lipid material and appears to synthesize lipofuscin. The tips of the excretory cells swell, fill with granules, and break off in the form of vesicles which are periodically expelled in clouds from the nephridiopores. Glycogen is present, especially in the ciliated cells of the tubule and coelomostome.Granules of a lipoid nature accumulate in (or between) cells of the nephridia, epidermis, and some regions of the gut, and may be excretory. Lipid granules also appear to be synthesized by coelomocytes which eventually end up in masses in the ventrolateral coelomic cavities of the tail.The nephridia act as gonoducts, but show no seasonal variation in either size or histological structure.

Notes: The ascophoran Pentapora foliacea was studied from epoxy sections of skeletal and soft (hard-soft) tissues. The basal wall is double, indicating the colony grew as two independent layers, back to back. The structure of the vertical walls and interzooidal communication organs indicates that zooids were budded in the usual way as in most encrusting cheilostomes. Secondary layers of the frontal wall are of acicular aragonite. The ovicell develops as a flattened cuticular bladder in early ontogeny; the aragonitic layer of the frontal wall later engulfs it. A median vesicle, an evagination of the vestibular wall, is present but the eggs may be supplied with sufficient yolk to nurture the embryo. The overall ovicell structure is similar to that of hyperstomial ovicells in other cheilostomes.

Notes: This investigation describes the pre-natal morphogenesis of the type I pneumocyte subsequent to its differentiation from pulmonary epithelium. Cells lining subpleural alveolar septa were photographed from serial sections with the electron microscope, and a three-dimensional representation of each cell was obtained by transferring the contours of the cell membranes from montages to transparent plastic sheets which were then spaced to scale and stacked. The results of this study indicate that: The nascent blood-air barrier of a 50-day reconstructed cell was twice as thick as the average definitive barrier; definitive barrier thickness was observed in some areas in a 63-day reconstructed cell; the amorphous component of elastic tissue which appears peripherally in septal connective tissue during pre-natal morphogenesis may be directly juxtaposed to the basal lamina of the alveolar epithelium; the orientation of the cell junction between a pneumocyte and its neighboring cells, as observed in sections of alveolar septa, changes as the contour of the pneumocyte changes from simple abutment to overlapping patterns.

Notes: The fine structure of the mid-gut musculature of the desert locust, Schistocerca gregaria is described and compared with that of the visceral muscles of other species. The gross morphology and fine structure of the nervous system which supplies the mid-gut muscle fibres is described.

Notes: The integument of Paranthessius anemoniae has been studied with light and electron microscopy. A cuticle with clearly defined epicuticular, exocuticular and endocuticular regions overlies a cellular hypodermal layer. The distribution of carbohydrate, lipid and protein components of the cuticle were demonstrated histochemically. Parabolic striations in oblique sections of cuticle suggest that its molecular architecture fits a “twisted sheet” theory proposed for other species.Arthrodial membranes at body and limb joints have a homogeneous structure, lacking exocuticle and endocuticle. Subcuticular glands appear to secrete substances thought to be responsible for the immunity which Paranthessius seems to have to the nematocysts of its host. Small hairs, situated in cuticular cups which occur over the dorsal body surface are considered to function as rheoreceptors.

Notes: The larval epithelium of the sea urchin, Lytechinus pictus, consists of squamous cells and bands of columnar epithelial cells bearing cilia. During metamorphosis this tissue undergoes a series of rapid, complex changes. Through the scanning and transmission electron microscope, we describe and analyse these changes. The changes can be divided into three steps. (1) The larval arms bend away from the left side of the larva, exposing the urchin rudiment. Cells which are identical to smooth muscle cells are in a position to bring about this bending. (2) The squamous epithelial cells assume a cuboidal shape. This change in shape results in the collapse of the larval epithelium onto the presumptive aboral surface. These cells possess a subapical band of microfilaments. The cellular shape change but not the bending of the arms is reversibly inhibited by Cytochalasin B. These observations suggest a mechanism for this change. (3) The former lining of the vestibule of the urchin rudiment comes to lie over the collapsed larval tissue and forms the adult epithelium. At this point, after only one hour, the larva has assumed the external shape of an adult sea urchin.

Notes: The mucous gland of the red-spotted newt, Notophthalamus viridescens viridescens, Rafinesque was examined by histochemical and ultrastructural techniques and its cytological responses to various hormonal conditions were studied. Its secretory epithelial cells produce and release in merocrine fashion a neutral, unsulphated mucosubstance. The secretory epithelium is bounded peripherally by a thin, but apparent non-functional, myo-epithelium. The duct of this mucous gland consists of a single keratinized tubular cell that extends from the neck region of the gland to the surface of the epidermis.Mucous secretion is absent or greatly reduced on the skins of newts maintained under laboratory conditions for a few weeks but reappears after injection of ovine prolactin. Mucous glands in laboratory conditioned animals show a 4-fold increase in volume brought about by the engorgement of their epithelial cells with secretory granules. Ovine prolactin reduces the volume of the glands to unconditioned levels with a corresponding reduction in granular content, suggesting that prolactin functions in the release of the granules. This view is reinforced by the findings that autotransplantation of the pituitary gland prevents the conditioning effect and that glandular volume increases in auto-transplanted animals given ergocornine. Granular accumulation begins also in hypophysectomized newts but ceases after a week, indicating the need for some hypophyseal factor in the synthesis as well as the release of the granules. Ovine prolactin restores mucous glands of hypophysectomized newts to the unconditioned state. Contrary to earlier findings, ovine prolactin induces a reduction in the volume of the mucous gland in thyroidectomized newts.

Notes: The process of coelomic pouch formation in Pisaster ochraceus was studied with light microscopy, transmission and scanning electron microscopy and time-lapse cinemicrography as well as with the drug cytochalasin B. As in most asteroids, the paired coelomic pouches of Pisaster ochraceus are formed from outpocketing of the archenteron.Arrays of 50 Å microfilaments are found in the presumptive coelomic pouch cells at the apex of the archenteron as well as in the filopodia of the mesenchyme cells. Both cell types undergo active movements throughout the entire process. Treatment of embryos with cytochalasin B (CCB) during coelomic pouch formation results in the loss of cell movements and the regression of the coelomic pouches; this is accompanied by the loss of microfilament arrays in both cell types. Cell movements and microfilament arrays reappear on removal of CCB and coelomic pouch formation resumes.Our evidence suggests that the microfilaments in the presumptive coelomic pouch cells provide the main force for the outpocketing movement. The major role of the microfilament arrays in the filopodia of the mesenchyme cells associated with the coelomic pouches is to determine the definitive shape and location of the pouches.

Notes: Morphological changes of the ovary of the Chinese cobra, Naja naja, throughout the annual reproductive cycle are described. A single clutch of between 6 and 22 eggs is produced in late June. From July to the following April the ovary remains quiescent and contains small previtellogenic, hydration stage follicles. The growth of an ovarian follicle from a primary oocyte to maturation and ovulation is estimated to take three years. The histology of the germinal epithelium and the follicular granulosa shows seasonal changes correlated with the growth of the oocyte. During the quiescent period, the germinal epithelium lacks mitotic activity, but during April, when yolk deposition and rapid growth of the preovulatory follicles take place, the germinal epithelium shows intense mitotic activity. The growth of the smallest hydration stage follicles, and the occurrence of cytoplasmic bridges between the pyriform cells of the granulosa and the developing oocyte, also appear to increase during this period. The possible function of the pyriform cell is discussed and the literature on the origin and fate of these cells in the squamate ovary is reviewed. Postovulatory follicles (corpora lutea) and two types of atresia are described and compared with what is known of these structures in other reptiles.

Notes: An experiment was undertaken to determine which sensory structures of the mouse embryo inner ear developed from what portion of the mouse otocyst. Otocysts of gestation days 10, 11, 12 and 13 were divided by surgical dissection into six anatomical groups: dorsal, ventral, anterior, posterior, medial and lateral halves. They were organ cultured separately. After a period of ten days, the explanted tissues were harvested and processed histologically for microscopic analysis. The surgical control specimens fixed at the time of explanation were composed of undifferentiated ectodermal cells for tissues of gestation days 10, 11, and 12. Otocysts of gestation day ten showed no gross morphological differentiation. Otocysts of gestation days 11 and 12 showed, during the course of their subsequent growth, that the three semicircular ducts and their associated cristae developed from the dorsal and lateral halves. Only the anterior and posterior canals and cristae originated from the medial portion. The posterior half gave rise to the posterior crista and the anterior half provided for the development of the anterior and lateral cristae. The cochlear duct and its sensory epithelium developed in all the anatomical groups except the dorsal half. The utricle developed in the dorsal section of the middle third of the otocyst, while the utricular macula developed in the anterior half of the same section of the otocyst. The saccule and its macula differentiated from the ventral section of the middle third of the anterior half.

Notes: The pineal-paraphyseal complex of sea turtles is an impressively large structure which projects dorsally and anteriorly above the prosencephalon. The complex was examined by light microscopy in several age classes of green sea turtles (Chelonia mydas) and from juvenile loggerhead turtles (Caretta caretta). The paraphysis is extensively fused to the distal portion of the pineal body, suggesting an interrelated function for these two tissues. No duct or canal was observed connecting the pineal lumen to the third ventricle. Two pineal cell types are described which appear to correspond to the neuroglial supportive cells and the secretory rudimentary photoreceptor cells of other amniotic vertebrates. A possible luminal secretion in the form of apical protrusions is produced by the latter cell type. No typical photoreceptive outer segments were observed.

Notes: The fine structure of the wax gland of Anomoneura nymph and its metamorphic change were investigated. In the nymph, this organ encircles the anus, and consists of two kinds of cells, derived from epidermal cells: (1) very tall, slim wax cells, which produce and secrete the wax, and (2) flat interstitial cells found among the wax cells. The whole gland is covered by a wax-secreting cuticle with a delicate surface sculpture. Each wax cell has a long, wide duct which opens at the cuticle and penetrates the entire cell. Its cytoplasm is rich in mitochondria and smooth endoplasmic reticulum while that of interstitial cells contains rough endoplasmic reticulum. During each nymphal molt, the cluster of primordial wax gland cells  -  derived from the epidermis  -  proliferates rapidly and forms the gland of the next instar. The gland of the preceding instar meanwhile degenerates. Interstitial cells play an important role in cuticle formation and shedding at each molt. These cells alone produce and deposit the new cuticle of the next instar; the wax cells, specialized for wax production, cannot produce cuticle. The apical portion of the wax cell is cut off from the main cell body by growth of the surrounding interstitial cells. Thereafter, the wax cells degenerate, resulting in the rapid disappearance of the previous instar's wax gland. Adults lack this gland entirely.

Notes: The dorsal ventricular ridge (DVR) is a subcortical, telencephalic structure in reptiles and birds that protrudes into the lateral ventricle. The structure of DVR has been studied in the red-eared turtle (Pseudemys scripta elegans) in Nissl and Golgi preparations. The DVR in Pseudemys is divided into the anterior dorsal ventricular ridge (ADVR) and the basal dorsal ventricular ridge (BDVR) by the dorsal branch of the middle ventricular sulcus. The structure of ADVR has been examined in detail.The ADVR is divided into four regions with distinct boundaries termed dorsal area, medial area, ventral area and central area. Dorsal area, medial area and ventral area border on the lateral ventricle; central area lies deep to the other areas. Three classes of neurons are found in Golgi preparations of ADVR. Juxtaependymal cells have somata near the perikarya of ependymal cells; their dendrites are found primarily in a periventricular fiber zone. Aspiny neurons were observed only in the dorsal half of ADVR and appear to be restricted to deep regions of the ridge. These multipolar neurons are rarely encountered in Golgi preparations, and the observed distribution may not represent their actual distribution in ADVR. The majority of the cells observed in ADVR are spiny neurons with dendritic fields that range from stellate to double-pyramidal. Cells in this class may be subdivided on the basis of axonal morphology into at least two groups, but further studies are needed to determine the range of axonal morphology exhibited by these neurons.An analysis of the distribution of these cell types in Golgi material shows that dorsal area, medial area and ventral area are organized in four zones concentric with the ventricular surface. Central area apparently lacks a concentric pattern of organization. Zone 1 is a periventricular fiber band that contains juxtaependymal neurons and ascending dendrites of zone 2 spiny neurons, and it may serve as a structural substrate for segregated input onto these cell populations. Zone 2 contains clusters of spiny neurons with apposed somata, which vary in size and distribution between areas. Dendrites of zone 4 neurons are also found in the deep half of zone 2. Zone 3 is a cell-poor region which lies at the center of a region of overlapping dendritic fields of zone 2 and zone 4 neurons. Zone 4 contains predominantly spiny neurons (aspiny neurons are found only in the dorsal half of ADVR) which are either isolated or in small clusters with apposed somata. Dendrites of zone 2 cells extend superficially into zone 4, so that the deep portions of zone 4 may be a substrate for segregated input to zone 4 neurons. These zones are differentially elaborated in each area. Central area, by contrast, consists of scattered spiny and aspiny neurons among fibers connecting ADVR and the lateral forebrain bundle.A comparison of these findings with the ADVR of snakes (Ulinski, '78a,b) shows both similarities and differences in DVR organization in the two taxa. Although snakes lack areal divisions, ADVR is organized in four concentric zones (zones A-D). Zones A and B resemble zones 1 and 2 in turtles, consisting of a superficial fiber zone and a subjacent cell cluster zone. The clusters are smaller in snakes than in turtles. However, snakes lack a cell-poor band deep to zone B, and dendrites of cells in zone C enter zone A. Thus, there are differences in both areal and zonal dimensions of ADVR organization in turtles and snakes.

Notes: Ultrathin sections of the nasal barbel of the channel catfish, Ictalurus punctatus, were studied in the electron microscope and the fine structure was compared to that of barbels of other teleosts and to the mandibular (dentary) barbels of I. punctatus. While the overall histology of the nasal barbel is similar to that of barbels described previously, this study revealed far greater cellular complexity and variability than was previously reported. A layer of stratified epidermal cells rests upon a connective tissue dermis containing a cartilage rod, a large number of nerve fibers and numerous blood vessels, fibroblasts and pigment cells. Taste buds are present in the epidermal layer. This layer was found to contain probably 16 kinds of cell types, several of which may represent transitional stages, in addition to taste bud cells. Observations were made pertaining to innervation and cell types in the taste buds. A new terminology for designating the barbels of I. punctatus is suggested.

Notes: When cervical segments 14 to 15 of the chicken spinal cord are cut transversely and studied by routine histological and histochemical methods, an onion-shaped region, filled with thread-like fibers, if seen to surround the ependymal cells of the central canal and to be bounded laterally by the neural elements of the spinal gray matter. This area is negative for succinic dehydrogenase, beta-hydroxybutyrate dehydrogenase and cholinesterase activity, but very strongly periodic acid-Schiff positive. Diastase controls show the positive material to be glycogen. Parasagittal sections through this cervical region and into the upper thoracic cord, show the glycogen-rich region to extend longitudinally throughout the region. Because of its location and histochemical characterization, which, He similar to that of the ventral portion of the glycogen body, the term brachial glycogen budy is proposed for this structure.

Notes: Anterior dorsal ventricular ridge (ADVR) is a major subcortical; telencephalic nucleus in snakes, Its structure was studied in Nissl, Golgi, and electron microscopic lrerarations in several species of snakes. Neurons in ADVR form a homogeneous population. They have large nuclei, scattered cisternae of rough endoplasmic reticulum in their cytoplasm, and bear dendrites from all portions of their somata. The dendrites have a moderate covering of pedunculated spines. Clusters of two to five cells with touching somata can be seen in Nissl, Golgi, and electron microscopic preparations. The area of apposition may contain a series of specialized junctions which resemble gap junctions. Three populations of axons can be identified in rapid Golgi preparations of snake ADVR. Type 1 axons course from the lateral forebrain bundle and bear small varicosities about 1 μ long. Type 2 axons arise from ADVR neurons and bear large varicosities about 5 μ. long. The origin of the very thin type 3 axons is not known; they bear small varicosities about 1 μ. long. The majority of axon terminals in ADVR are small (1 μ. to 2 μ long), contain round synaptic vesicles, and form asymmetric active zones. This type of axon terminates on dendritic spines and shafts and on somata. A small percentage of terminals are large, 5 μ in length, contain round synaptic vesicles, and form asymmetric active zones. This type of axon terminates only on dendritic spines. A small percentage of terminals are small, contain pleomorphic synaptic vesicles, and form symmetric active zones. This type of axon terminates on dendritic shafts and on somata.

Notes: Tooth development and replacement in fetal and adult viviparous caecilians (Amphibia: Gymnophiona) are described and analyzed according to current theories of tooth succession. The fetal dentition differs from that of the adult in morphology, position, and function. Teeth are used by fetuses to scrape the oviducal epithelium, thus stimulating the secretion of a nutrient substance. Fetal dentitions vary in morphology and position in different species. The ontogeny of teeth of several species is described and the patterns of addition of loci and of replacement are analyzed, Loci are added both posteriorly along the jaw and between existing loci as the jaw grows prior to ossification; subsequently addition is restricted to the posterior part of the jaw. Tooth replacement is alternate. The several rows and patches of teeth are the result of retention of replacement series on the dentigerous elements. Tooth development and replacement in a series of juveniles and adults of different sizes in a single species are also considered. Post-fetal patterns of development and replacement are similar to those seen in larvae and adults of oviparous species. Variation in numbers of teeth and proportions of teeth at particular stages occurs ontogenetically and among individuals of the same size, though proportions occur in a similar pattern throughout the series. The general pattern of tooth replacement in fetuses and adults can be explained by either Edmund's Zahnreihen theory or by Osborn's Tooth Family theory, but replacement in fetal tooth patches and the fetal-adult dentitional transition are explained by neither.

Notes: In Brallchiobdella pentodonta Whitman meiosis begins in follicles containing 16 spermatogonia. In each follicle the spermatogonia are connected by cytoplasmic bridges to a central anuclear cytoplasmic mass or cytophorus. They develop synchronously. Synaptonemal complexes are present in the primary spermatocytes. Spermatids contain a large globoid paranuclear body consisting of an acrosomal granule and coiled tubules which evidently receive the contents of the acrosomal granule and are considered the acrosome carrier. The spermatids separate from the cytophorus only when differentiation is completed.The ripe spermatozoon is relatively long. It has anteriorly the coiled tubules, followed by the nucleus, the mitochondrial sphere and the distal centriole from which the flagellum originates, A coiled ribbon-like structure encloses the flagellum along its entire length while a manchette of microtubules surrounds all the other structures of the sperm.

Notes: The muscles and bones of the pectoral fin of Serrasalmus nattereri, the piranha, resemble those of generalized, lower teleosts with specializations related to a body shape adapted for high-speed carnivory; the pectoral fins being highly mobile with strong ligaments to the rays. The presence of two occipital nerves appears primitive, while the emergence of the subclavian artery within the branchial cavity, as in Gasteropelecus sternicla, appears specialized. The muscles and bones of the latter fish, a fresh-water flying fish, are specialized for self-propelled, aerial flight in the fusion of the right and left girdles greatly expanded for insertions of complex appendicular (flight) muscles, and in the consolidation of the rays and radials into one functional unit moving vertically in flight though contraction of vertical, massive ventral flight muscles. The bony pectoral anatomy of Electrophorus electricus, the electric eel, is specialized in having a mobile joint between the primary girdle and the cleithrum, the former being suspended vertically from the cleithrum by ligaments. The proximal radials and rays are very numerous and vertically aligned. The cleithrum is shaped to accommodate the extensive sternohyoid and pharyngocleithral muscles. The sheet-like appendicular muscles extend beyond the special joint and control its movement. The deeper muscles do not cross this joint. The arterial system is specialized in lacking a deep brachial artery.

Notes: The cibarial food pumps of aquatic Heteroptera contain specialized epipharyngeal triturating devices. In the Naucoridae, striated bands and transverse plates triturate particles against the underlying hypopharynx. Anterior to them lie a pair of oblique folds which play an accessory role. The gross morphology of these devices is very similar in representatives of five genera of typical Naucoridae (Ambrysus, Pelocoris, Limnocoris, Cataractocoris, Cryphocricos) and differs from that of the atypical genus Aphelocheirus.The scanning electron microscope reveals additional differences between Aphelocheirus and the typical genera as well as variations, among the latter, which are not visible with the stereoscopic microscope. The oblique folds of the typical Naucoridae are well developed and contain processes for trapping particles; in three genera the region posterior to the folds is also modified. In Aphelocheirus only the latter region appears to trap particles, and the oblique folds are smooth and weakly developed. The striated bands of all genera bear ventral ridges arranged into transverse zones with precise patterns. The fourzoned bands of Aphelocheirus have a very different pattern than the two-zoned bands of the other genera. Among the latter, Cryphocricos has a simpler pattern of ridges than the other typical Naucoridae. The ventral surfaces of the transverse plates are highly modified in Aphelocheirus and less so in the other genera; those of Cryphocricos differ from those of the other Naucoridae.The fine structure of the cibarial epipharynx supports the views of some systematists that (1) Aphelocheirus should be placed in the monogeneric Family Aphelocheiridae rather than in the Naucoridae, (2) Cryphocricos represents a different subfamily than the other four typical Naucoridae, and (3) Cataractocoris belongs in the same subfamily as Ambrysus rather than with Cfyphocricos.

Notes: Appearance of collagen fibrils in the cuticle was seen by electron microscopy to be preceded by fonnation of a finely filamentous matrix material. At first, the fine filaments of the matrix are unorganized. However, signs of orthogonal ordering soon appear in the most superficial portion of the cuticle, and subsequently appear more basally and closer to the underlying epidermis. Meanwhile, fibrils of different staining properties and identifiable as collagen begin to be deposited in the superficial portion of the cuticle, the same region which first showed organized fine filaments. Then, like the fine filaments before them, the collagen fibrils polymerize more basally. Collagen appears to polymerize on the preformed skeleton of fine filaments as though the fine filaments caused the collagen to assemble. Neither the polymerization nor ordering of collagen fibrils seems to require direct cellular intervention but occur first in that portion of the cuticle which is furthest away from the underlying epidermis. The fine filaments may be self ordering, extracellular macromolecules which in turn determine the polymerization of collagen fibrils.

Notes: Oogenesis of the fresh-water triclad Dugesia dorotocephala has been studied by electron microscopical methods, with particular regard to the genesis and composition of the so-called “Balbiani body.” Its origin is clearly recognizable in young oocytes where the few mitochondria present seem to gather at the level of the perinuclear ooplasm. Here they surround dense masses of finely granular, fibrillar material probably coming from the nucleus. During the previtelloge ic period, mitochondria rapidly increase in number while the dense masses progressively dissolve.In the vitellogenic oocytes the Balbiani body shows its final configuration: it appears as a large area (up to 15-20 pm in diameter) consisting of innumerable densely packed mitochondria, some smooth vesicles and free ribosomes. This aggregate of cytoplasmic organelles remains unmodified in the mature oocytes.The function of the “Balbiani body” of D. dorotocephala is as yet unclear; it can only be asserted that it is not correlated with yolk production in which the endoplasmic reticulum and the Golgi complex are involved.

Notes: Whole ovaries from 16-day fetal mice were cultured for 6 to 20 days and then transplanted to the kidneys of ovariectomized adult mice where they remained for one to four weeks. After three weeks in the host's kidneys, many follicles developed within the transplants and became vesiculated. Many of the oocytes within these follicles had formed the first metaphase spindle of meiosis and several had completed the first polar body. Host mice bearing transplants that contained vesiculated follicles showed uterine stimulation and keratinization of their vaginae. However, ovaries that had been in culture for more than ten days before transplantation showed a limited response to the gonadotropins and never matured sufficiently to stimulate the host's reproductive tract. No ovulations occurred in any of the transplants.

Notes: New data on the brain of Latimeria indicate that previous estimates of the brain weight were too high by a factor of two. Our data suggest a brain weight of 1.1-1.5 grams for a specimen with a body weight of 30 kilograms. Quantitative data on major divisions of the brain are presented for the first time, and the relative size of the major brain divisions is similar to that of sturgeons and generalized sharks (such as hexanchids and squalids). Examination of brain component weight (s): body weight plots in a sample of non-teleost actinopterygian fishes indicates that all major divisions of the brain, except the telencephalon, are larger than in Latimeria. Brain component sizes in Latimeria are more similar to those extrapolated for amphibian brains than to those for actinopterygians. However, the cerebellum of Latimeria is considerably larger than that of amphibians.

Notes: The Champy-Maillet osmium tetroxide-zinc iodide technique and a new method using azur B-sodium thioglycolate were used to study the general nervous tissue structure in planarians. A subepidermal and a submuscular nerve plexus, partially reported by earlier authors, are described, and a gastrodermal plexus is reported for the first time in triclads. The possible functions for each one of these plexuses are discussed. By the Champy-Maillet method, the innervation within the parenchyma appears as an array of numerous single nerve fibers that course between the parenchyma cells making apparent synaptic contacts. The pharynx has outer and inner nerve nets similar in structure to the submuscular nerve plexus. Both nerve nets are connected to each other by radial nerves.The central nervous system has a sponge-like structure with many lacunae filled with cell bodies, dorso-ventral muscle fibers, parenchymal cell processes and excretory ducts. The existence of this sponge-like nervous tissue structure is discussed in relation to the still incomplete centralization of the nervous tissue in these organisms, to the lack of a true vascular system and to the acoelomate level of organization. A comparison with the nervous tissue structure of more advanced groups like polyclads and nemertines is suggested.

Notes: Secretion in the salivary glands of Gromphadorhina portentosa involves three cell types: parietal cells, secretory cells, and duct cells. The organization and role of the parietal and secretory cells are here considered. Parietal cells have numerous mitochondria, indicating an active metabolic role and the subsequent production of ATP. Plasma membrane invaginations and intracellular ductules containing microvilli appear to function in the absorption of solutes from the hemolymph and finely-tapered ductules. Secretory cells contain abundant rough endoplasmic reticulum, the three forms (stacked, vesicular, and diffuse) of which appear to develop sequentially during maturation. Secretory vesicle formation is asynchronous between adjacent secretory cells, and apparently the large vesicles often coalesce. The secretory vesicles also show differing degrees of electron density, indicating distinct biochemical composition.

Notes: Studies were undertaken of the microcirculation and histology of the gill of Protopterus aethiopicus as a prerequisite for elucidating the function of the gills in a bimodal respiratory system. The lamellae of the gill-bearing arches (I, IV, V, VI) resembles the arborescent external gill of the larval amphibian rather than the gill of the teleost or selachian.The arterio-arterial system (a-a) of the gill consists of an afferent artery, a series of large capillaries, and an efferent artery on each of the primary, secondary and tertiary lamellae. There are no pillar cells and the loose capillaries are covered with a multilayered epithelium. While living in water, the minimum distance for gas exchange is of the order of 5 μ. An afferent-efferent arterial shunt at the base of each primary lamella may be involved in control of lamellar blood flow and the resistance of the gill vasculature.The arterio-venous system originates primarily from the efferent side of the arterio-arterial system and drains into large branchial veins. Numerous contractile cisternae, interposed between intercellular channels and veins, presumably function as micropumps that collect fluid from intercellular epithelial spaces and inject it into the venous circulation.During aestivation, the epithelial layer of the gill lamellae becomes thinner. The entire gill vasculature, including the capillaries and afferent-efferent shunts on arches IV-VI, are very dilated which presumably promotes blood flow through these gill arches to the lungs.

Notes: The movements of the shoulder girdle of eight adult cats during overground stepping were studied, using standard slow motion cinematographic techniques. The patterns of activity of shoulder muscles were examined, using simultaneous intramuscular electromyography. Walking, trotting and galloping steps were analyzed from digitized single motion picture frame images. Angular movements of the shoulder girdle consist of biphasic flexion and extension of the shoulder joint and a monophasic flexion-extension alternation of the scapula on the thorax during each step cycle. In addition, the center of the scapula moves craniad during the swing phase and caudad during the stance phase with respect to a fixed reference point on the animal. Similar vertical movements of the center of the scapula also occur in each step cycle. Results of EMG studies of the 17 muscles capable of acting on the shoulder girdle indicate that three overall patterns of activity are found: (1) a pattern typical of extensor muscles, active during all the extension epochs; (2) a pattern typical of flexor muscles, active during the flexion epoch; and (3) a biphasic pattern of activity, active twice in each step. These data are used, along with a re-examination of previous models of the mechanics of the shoulder girdle of carnivores to examine the function and mechanics of shoulder motion. It is concluded that the rotary and translatory movements of the shoulder girdle during stepping combine to enhance step length.

Notes: The most striking morphological feature of the ovarian epithelium of Cymatogaster is the presence of intercellular dilations during much of the year. These dilations increase markedly in volume during the several months prior to ovulation and fertilization, and decrease in volume during the months of embryogenesis and gestation. The epithelium then returns to its initial, relatively undifferentiated state. The extracellular material within the dilations likely is synthesized in the cells adjacent to or within the dilations. Apparently most of this material is released into the ovarian lumen when the apicolateral margins of adjacent epithelial cells pull apart; possibly it serves as nutrient for developing embryos.In addition to supporting embryogenesis, the ovarian epithelium also apparently is involved in sperm storage. Sperm are maintained within pockets in the ovarian epithelium for the several months between insemination and fertilization. The cells lining the sperm pocket do not develop the intercellular dilations characteristic of most of the ovarian epithelium, and sperm remain associated only with the sperm pocket cells.

Notes: The antennae of Folsomia candida and Hypogastrura copiosa are provided with tactile hairs on all four segments and, in addition, with chemoreceptors on segments three and four. The cuticle of the thin-walled chemo-receptors of Folsomia, as in most other insects, is penetrated by many small pores uniformly distributed over the surface. In contrast, the cuticle of the thin-walled chemoreceptors of Hypogastrura is unusual in that a band of cuticle without perforations spirals around, or partly around, the hair. The cuticle between the spirals is very thin, and has extremely small openings in it. The tip of the antenna of Folsomia is thin, permeable to dyes in aqueous solution and apparently glandular. The antennal tip of Hypogastrura is definitely glandular.

Notes: After hemisection of the spinal cord and medulla oblongata, a projection has been traced to the inner half of the tectal white of the tiger salamander, using Fink-Heimer degeneration staining. By microelectrode recording it was found that the tectal projection forms a topographic somatosensory map of the contralateral half of the body. This map is in register with the overlying retino-tectal visual projection. Using the Falck-Hillarp technique, it was found that the somatosensory tectal input is associated with yellow-fluorescing 5-hydroxytryptamine fibers.

Notes: A comparative morphological study concerning typology and topography of chemoreceptors on the prothoracic legs of Calliphora vicina, Phormia terranovae and Musca domestica has been carried out. The typological criteria of Grabowski and Dethier ('54) and Hansen and Heumann ('71) were used. A single criterion, the shape of the tip, was used to define the different types of chemoreceptors.A-hairs have a rhombic pore at the side of the tip; B-hairs have an oval pore at the tip apex and D-hairs have a rectangular pore under an undulated, cap-like structure at the hair tip. A-, B-and D-hairs were found in the tarsomeres of Phormia; in Musca and Calliphora only B- and D-hairs were found. An opening and closing mechanism may operate on the pores of the tips of the chemoreceptors. Chemoreceptors were counted and a topographical map was completed, using SEM-techniques. Topographical maps are of value in electrophysiological and behavioural research, where only a limited optical magnification is possible.

Notes: The structures of the lantern tracheoles of three genera of flashing fireflies are compared. All three genera have stiff, reinforced tracheoles which resist folding or collapsing under conditions which flatten more typical tracheoles. This common specialization supports the hypothesis that the tracheoles play a major role in flash control in these fireflies, especially as the morphological basis of the stiffening is different in the three genera. Study of the tracheoles of other tissues reveals that there is great variety in structure and flexibility of these vessels from tissue to tissue and organism to organism, suggesting that tracheolar specialization may be a general phenomenon, with the fine structure of these air tubes being tailored to the particular demands and conditions of the tissues in which they are found.

Notes: Tibiotarsal segments of 12-day chick embryos homozygous for the crooked neck dwarf gene (cn/cn) were examined histologically following routine methods of preparation. The myogenic mass fails to divide into separate muscle bundles during the early stages of differentiation. Myoblasts and myotubes are observed, although the proportion favors the mononucleate cell population. Multinucleate myotubes are often wavy in appearance and many contain eosinophilic cytoplasmic inclusions. The entire tissue mass of mutants appears more compacted than in control limbs. Poor organization of muscle appears related to the lack of a suitable connective tissue system. Epimysia, perimysia, and subcutaneous connective tissue fail to develop properly. Tendons are poorly developed or absent. Comparisons between mutant and control embryos show no differences in peripheral innervation. Nerve fascicles penetrate deeply into the developing muscle of both species. The distribution of vascular elements is seemingly normal also. Skeletal muscle of cn/cn embryos is capable of differentiating to the myotube stage, after which it undergoes cellular degeneration without achieving a functional state. Comparisons of this mutant with alleged chemical phenocopies show important differences.

Notes: The histology and carbohydrate histochemistry of eight teleostean stomachs are compared. Three gross anatomical types of stomachs are described and their shapes appear to correlate somewhat with feeding habits. Each type can be divided histologically into a corpus and pylorus. Gastric glands, containing only one cell type, occur in the copora of all species, but are present in the pylori of esocids only. As a single cell can produce both enzymes and hydrochloric acid such cells may be comparable to those of amphibians but not mammals. Lamina propria and submucosa are indistinctly separated in corpora but better defined in pylori by an intervening muscularis mucosa. The arrangement of the muscularis into inner circular and outer longitudinal layers is the opposite of that seen in the esophagus. Gastric mucous cells show species variations in localization of epithelial mucosubstances, which in broad terms are recognized as sulfomucins, sialomucins and neutral mucosubstances. A piscivorous diet does not appear to demand any particular type of carbohydrate. Within the Centrarchidae, gastric pit cells vary in carbohydrate content from only neutral mucosubstance to only weakly acidic sulfomucin; two species contain both types. A positive PAS reaction on the surface of gastric epithelial cells is suggestive of a striated border and thus possibly absorptive function. The absence of stomachs in some teleosts and the evolutionary and dietary significances are discussed.

Notes: The American cockroach has a total of 368 muscles inserting on the post-coxal segments of its legs. By using a narrow morphological definition for delimiting individual muscles, it is shown (i) that the protrochanteral musculatures (23 muscles/leg) differ from the essentially identical meso- and metatrochanteral musculatures (24 and 26 muscles/leg) in number and disposition of extensors and in having a completely different flexor composition, and (ii) that the musculatures of the more distal segments of the legs are completely serially homologous, there being 2 muscles for moving each femur, 23 for each tibia, 7 for each first tarsomere, and 5 for each of the paired pretarsal claws. In all six legs, the trochanteral and tibial musculatures each contain single slender muscles that may be acting proprioceptively to measure the angular displacements between, respectively, the coxas and trochanters, and the femurs and tibias. Neurological and phylogenetic considerations are used to demonstrate why a narrow morphological definition should be employed, and why the widely used functional definition of Snodgrass ('35) is not only fallacious on evolutionary grounds, but also leads to making erroneous conclusions regarding the manner in which insect musculature is controlled by the insect central nervous system. Finally, it is hypothesized that the physiological limitations imposed by having an open circulatory system and the problems inherent in the neural control of large muscles may have been major evolutionary factors in forcing insects to use many slender muscles to control their body movements.

Notes: Among piscivorous cichlids consistent differences have been recorded between ambush and pursuit hunters with respect to electromyographic, kinematic, pressure and behavioral profiles during prey capture by high speed inertial suction. Piscivorous cichlids possess a repertoire of at least two patterns of prey capture, each of which is characterized by an extreme regularity of the kinematic, pressure, electromyographic and behavioral profiles. The nature and locomotory behavior of the prey, visually analyzed by the predator during the prestrike stalk, determine which of the two preprogrammed patterns is recruited. Agile and elusive prey invariably will elicit a preprogrammed motor output (stereotyped motor pattern) that produces the greatest suction velocities in both ambush and pursuit hunters. The greater the kinematic and suction velocities, the greater the overlap of the firing sequences of antagonistic muscle complexes. The opercular and branchiostegal apparati function as an exceedingly effective anti-backwash device, damping potential fluid oscillations within the oropharynx. Mastication occurs by triphasic movements and actions of muscles of the upper and lower pharyngeal jaws in both ambush and pursuit hunters. The lower pharyngeal jaw is acted upon by a force couple of which the fourth levator externus on one hand and the pharyngocleithralis externus and pharyngohyoideus on the other hand are the antagonistic components. Furthermore, the lower pharyngeal jaw is suspended by a muscular sling, the tension of which can be modified continuously. It is postulated that the switch from insectivorous to piscivorous feeding regimes (and perhaps vice versa) is accomplished by very minor structural and functional modifications, because the modulatory multiplicity and total range of repertories of the feeding machinery of the two trophic groups overlap significantly. Piscivorous cichlids may not have arisen by orthoselection in gradually-changing lineages, but represent the differential success of subsets from a random pool of speciation events. Adaptive features identified as characteristic for piscivory could have evolved in multiple and independent lineages at a punctuational mode and tempo.

Notes: The infracerebral gland of Nereis is made up of three types of cells. C1 cells are hypertrophied pericapsular elements, whereas C2 and C3 cells have the morphological and cytological features of neurosecretory neurons. C2 and C3 cells give rise to centripetal “proximal processes” which extend into the brain through the midventral pocket formed by delamination of the brain capsule. Their “distal processes” terminate within the gland or its immediate vicinity. “Centrifugal fibers” arise from nerve cells located within the brain and appear to synapse upon the proximal processes of C2 cells in the region of the midventral pocket and in the ventral region of the brain. The cytology of C2 and C3 cells suggests that they are the source of distinct peptide hormones.

Notes: Exercise affects the growth of the dorsal longitudinal flight muscles in the tsetse fly. Examination of electron micrographs of flight muscles taken from flies subjected to enforced exercise, “ormal” exercise and no exercise reveals that both mitochondrial and myofibrillar fractions of the muscles are stimulated to grow at a faster rate by enforced exercise but that the mitochondria respond more rapidly.

Notes: Ultrastructure and shell formation in the testaceous ameba, Lesquereusia spiralis, were investigated with both scanning and transmission electron microscopy and X-ray microanalysis. The nucleus, surrounded by a fibrous lamina, contains multiple nucleoli. The cytoplasm, containing a well developed granular endoplasmic reticulum, also contains remnants of starch granules in stages of digestion. Spherical aggregates of ribosome-like particles may be seen. Golgi complexes seem to produce both a nonordered fibrous material and an electron dense vesicle. Only the latter appears to bleb off from the Golgi complex. X-ray microanalysis demonstration of silicon in Golgi vesicles and in some dense vesicles suggests that the fibrous component of the cisternae may take up and concentrate silica to form the electron-dense component of the vesicles. Membrane-bound siliceous crystals are often seen adjacent to the Golgi, suggesting either a Golgi origin or platelet formation in vesicles after release from the Golgi complex. Both electron-dense bodies and siliceous platelets are released from the cell by a process similar to apocrine secretion and may be seen outside the cell in route to the shell during shell morphogenesis. Shell development involves fusion of electron-dense bodies to form a matrix, positioning of siliceous platelets in this matrix parallel to the shell surface, and development of a system of matrix chambers. A particulate glycoconjugate is released to the shell surface upon rupture of the matrix chamber.

Notes: Stereological analysis of the ultrastructural composition of the pulmonary alveolo-capillary region of mice living at sea level compared with that of the same species (Phyllotis darwini) genetically adapted to life at 4,660 m reveals a trend at high altitude towards a greater volume percentage of tissue components. On a weight-specific basis, non-circulating tissue occupies a significantly greater volume in high-altitude mice, but air space and capillary contents are not correspondingly greater. Since the arithmetic mean thickness of the tissue layers and of the air-blood barrier are the same in the two altitudinal groups, the average alveolus must have a smaller volume in the high-altitude mice.Epithelial, endothelial, and erythrocyte surface areas per gram body weight are significantly greater in the high-altitude mice.Nuclear counts indicate that the larger lungs of mice adapted to high altitude are due to larger Pneumocyte I and II and endothelial cells rather than to an increase in the number of these cells. Hematocrits measured within the pulmonary capillaries in the two altitudinal groups were equal.An heretofore unrecognized feature of possible adaptive value is the surface/volume ratio of erythrocytes, which is similar for erythrocytes in alveolar space of mice at low and high altitudes but within lung capillaries is 14.7% greater at high altitude.

Notes: The neck region of the mature spermatozoon of Discus rotundatus is described. No evidence for a centriole or centriolar derivative is obtained. Nine striated coarse fibres and the two central fibres of the axoneme extend into the base of the implantation fossa. The axonemal doublet system is disrupted in the neck region. There are two fibrous accessory structures located between the central doublet and the striated coarse fibres.

Notes: Explants of 4.5-day-old chick embryonic neuroretinas with mesenchyme were exposed to Methotrexate (MTX) in medium 199 with embryo extract. Proliferative responses of the cultured neuroretinas were followed radioautographically by administration of 3H-thymidine to the cultures. The DNA synthetic, mitotic and pyknotic responses of the ventricular cells of the neuroretina were followed over a 16-hour period. The responses observed suggested that MTX caused a synchronization of the ventricular cells in the pre-mitotic phases with no direct inhibition of mitosis. Furthermore, prolonged exposure to MTX resulted in the accumulation of labeled pyknotic cells, indicating a decline in the regenerative capacity of the proliferative ventricular cells.

Notes: Successive tracheal cuticles of the dorsal longitudinal trunks are studied with the electron microscope. Minor differences seen at the light microscope level are seen as major qualitative and quantitative ones at the ultrastructural level. The larval and pupal cuticles are secreted by similar epithelial cells; these possess large polytene chromosomes. Cell division and possibly cell replacement occur prior to adult cuticle secretion. The findings are discussed in terms of cell specificity, intra- and inter-cellular pattern formation. This simple epithelium, the individual cells of which are capable of producing different cuticles, is interesting since the system is also shown to be responsive to hormone application.

Notes: Eggs of the common snapping turtle, Chelydra serpentina, were incubated at constant temperatures ranging from 20°C to 30°C, At hatching, the oviducts were absent or incomplete in males; the testes were differentiated. In females at hatching, the oviduct was intact hut in some cases the gonad retained bisexual characteristics. Three months after hatching, the ovary was differentiated and contained follicles. Eggs incubated at 20°C and at 30°C developed into females in 100% of the cases. At 26°C, 99% of the individuals were males; at 24°C, 100% were males. More males than females developed at incubation temperatures of 22°C and 28°C.

Notes: New blastozooids of Polyzoa vesiculiphora, the polysytelid ascidian are produced by pallial budding of three types depending on the method of “isolated bud” formation; stolonic, planktonic and intermediate types. Differences among each type of bud are attributed to behavior of test-vessels composing a part of the bud. Isolated buds produced by each type are essentially equal in terms of their internal structures and their subsequent fate, and develop independently of their parent zooids. New test-vessels originate directly from the epidermis of a “prefunctional zooid,” while the test-vessels derived from the parent zooid finally disintegrate. The new test-vessels extended with branching under the ventral side of a “functional zooid,” ascend to the lateral side of it and participate in bud formation. Budding regions exist in three dimensions on the lateral wall of the mantle of the functional zooid, especially the right posterior part. During the life cycle of one functional zooid, the stolonic type buds appear at early and/or aged stages. Appearances of the stolonic type buds in early stages tend to repress those of the planktonic types. The number of planktonic type buds formed on a functional zooid at the same time is many more than that of the stolonic type. Such budding features are discussed from the viewpoint of behavior of the test-vessel system.

Notes: There are two discrete lobes comprising the armadillo subman-dibular gland. These two lobes can be defined grossly, histochemically and morphologically with the light and electron microscope. The minor lobe stains more intensely with PAS and AB. When viewed in the electron microscope, the secretory granules of the acinar cells within this lobe appear mucous-like. The granules of the demilune cells are slightly different in appearance. The secretory granules of the acinar cells in the major lobe contain many dense foci embedded in a fibrillar matrix, a substructure not described previously. The demilune cells of this lobe contain secretory granules with a mucous-like structure which is consistent throughout the entire lobe. As in the minor lobe, these demilune cells stain very intensely with PAS and AB.

Notes: End-plate distributions have been determined for three frog muscles of different morphology in order to relate end-plate topography to spatial muscle structure and nerve branching. Koelle's cholinesterase technique was applied, both on whole muscles and frozen sections. The end-plates of the short parallel-fibered cutaneus pectoris muscle appeared to be located in short bands along the nerve branches. The nerve tree is restricted to a zonal area across the middle part of the muscle. Depending on the way the nerve branches, the end-plate bands form innervation patterns, varying from one single continuous band to multiple distributed bands. In the latter case one frequently observes that different end-plate bands do not run across the same longitudinal muscle fiber area, although the respective nerve branches run parallel across this area. The long parallel-fibered sartorius muscle has a wider nerve tree and exhibits the same phenomenon for close parallel nerve branches, but end-plate bands along parallel nerve branches far apart cover the same muscle fiber area. The end-plate distribution in the bipennate, short-fibered gastrocnemius is zonal throughout the muscle except in certain compartments containing tonic fibers. The end-plate zone centers around the inner aponeurosis about half-way between the muscle tendon junctions of the fibers and is visible only at the muscle surface where muscle fibers run over their entire length at that surface. The results are of general use in the electrophysiology of neuromuscular transmission because they illustrate how in certain twitch muscles neuromuscular morphology may help to localize end-plates.

Notes: When a larva of Haplothrips verbasci is ready to feed, it grasps the surface of the leaf with its pretarsi, sinks down between its front legs, lifts its head, and places the tip of its mouthcone against the surface. It then shortens its mouthcone and punches a hole in the epidermis by rapidly and repeatedly protracting and retracting its left mandibular stylet. The thrips then inserts its two maxillary stylets as a unit into the wound with a series of rapid thrusts and withdrawals, salivating continuously while doing so. When a food source in the epidermis or mesophyll is found, probing and salivation stop and cibarial pumping begins. Cytoplasm is sucked into the opening at the tip of the protracted stylets, up the food canal between them and into the cibarium.Probing and feeding can occur without mandibular intervention but uptake of liquid seems to require use of the mutually coadapted maxillary stylets, even when these are fully retracted.Prior to molting, the larva protracts its maxillary stylets maximally and, in the pharate state, seems incapable of feeding or drinking.Structures used in feeding are fully described and are shown to resemble those of Hemiptera except for the presence of maxillary and labial palpi and the absence of the loral lobes, right mandible and of a salivary canal between the protracted maxillary stylets. Seven single and 18 paired muscles function in the feeding act, nine less than in adults of the same species.Differences in the feeding mechanism of terebrantian and tubuliferous thrips are discussed and evidence is presented to suggest that the simplified and more highly specialized mouthparts of the latter insects are adaptations for feeding in confining spaces.

Notes: In contemporary entomology the morphological characters of insects are not always treated according to their phylogenetic rank. Fossil evidence often gives clues for different interpretations. All primitive Paleozoic pterygote nymphs are now known to have had articulated, freely movable wings reinforced by tubular veins. This suggests that the wings of early Pterygota were engaged in flapping movements, that the immobilized, fixed, veinless wing pads of Recent nymphs have resulted from a later adaptation affecting only juveniles, and that the paranotal theory of wing origin is not valid. The wings of Paleozoic nymphs were curved backwards in Paleoptera and were flexed backwards at will in Neoptera, in both to reduce resistance during forward movement. Therefore, the fixed oblique-backwards position of wing pads in all modern nymphs is secondary and is not homologous in Paleoptera and Neoptera. Primitive Paleozoic nymphs had articulated and movable prothoracic wings which became in some modern insects transformed into prothoracic lobes and shields. The nine pairs of abdominal gillplates of Paleozoic mayfly nymphs have a venation pattern, position, and development comparable to that in thoracic wings, to which they are serially homologous. Vestigial equivalents of wings and legs were present in the abdomen of all primitive Paleoptera and primitive Neoptera. The ontogenetic development of Paleozoic nymphs was confluent, with many nymphal and subimaginal instars, and the metamorphic instar was missing. The metamorphic instar originated by the merging together of several instars of old nymphs; it occurred in most orders only after the Paleozoic, separately and in parallel in all modern major lineages (at least twice in Paleoptera, in Ephemeroptera and Odonata; separately in hemipteroid, blattoid, orthopteroid, and plecopteroid lineages of exopterygote Neoptera; and once only in Endopterygota). Endopterygota evolved from ametabolous, not from hemimetabolous, exopterygote Neoptera.The full primitive wing venation consists of six symmetrical pairs of veins; in each pair, the first branch is always convex and the second always concave; therefore costa, subcosta, radius, media, cubitus, and anal are all primitively composed of two separate branches. Each pair arises from a single veinal base formed from a sclerotized blood sinus. In the most primitive wings the circulatory system was as follows: the costa did not encircle the wing, the axillary cord was missing, and the blood pulsed in and out of each of the six primary, convex-concave vein pair systems through the six basal blood sinuses. This type of circulation is found as an archaic feature in modern mayflies. Wing corrugation first appeared in preflight wings, and hence is considered primitive for early (paleopterous) Pterygota. Somewhat leveled corrugation of the central wing veins is primitive for Neoptera. Leveled corrugation in some modern Ephemeroptera, as well as accentuated corrugation in higher Neoptera, are both derived characters. The wing tracheation of Recent Ephemeroptera is not fully homologous to that of other insects and represents a more primitive, segmental stage of tracheal system.Morphology of an ancient articular region in Palaeodictyoptera shows that the primitive pterygote wing hinge in its simplest form was straight and composed of two separate but adjoining morphological units: the tergal, formed by the tegula and axillaries; and the alar, formed by six sclerotized blood sinuses, the basivenales. The tergal sclerites were derived from the tergum as follows: the lateral part of the tergum became incised into five lobes; the prealare, suralare, median lobe, postmedian lobe and posterior notal wing process. From the tips of these lobes, five slanted tergal sclerites separated along the deep paranotal sulcus: the tegula, first axillary, second axillary, median sclerite, and third axillary. Primitively, all pteralia were arranged in two parallel series on both sides of the hinge. In Paleoptera, the series stayed more or less straight; in Neoptera, the series became V-shaped. Pteralia in Paleoptera and Neoptera have been homologized on the basis of the fossil record.A differential diagnosis between Paleoptera and Neoptera is given. Fossil evidence indicates that the major steps in evolution, which led to the origin first of Pterygota, then of Neoptera and Endopterygota, were triggered by the origin and the diversification of flight apparatus. It is believed here that all above mentioned major events in pterygote evolution occurred first in the immature stages.

Notes: Studies of the response of adult mammalian and amphibian ventricle to injury have indicated the formation of a connective tissue scar in the place of the wounded or amputated muscle. It has been demonstrated that amphibian myocytes adjacent to a wound surface, unlike mammalian myocytes, have a proliferative capacity. In the present study, a minced cardiac muscle graft was placed into the adult newt ventricle in order to increase the number of myocytes near a wound surface. With such an increased number of reactive myocytes, it was thought a new wall consisting primarily of muscle might be formed. One-sixteenth to one-eighth of the ventricular apex was removed, minced and returned to the amputation surface of the ventricle. General histological and autoradiographic studies were conducted on two sham-operated animals and on five experimental animals which were killed at 5, 10, 20, 30, 50 and 70 days after surgery. Major events of the repair and reorganization of minced cardiac muscle included blood clot formation followed by necrosis of the blood clot and much of the muscle graft. By ten days, an apparent coalescence of muscle fragments and continuity of ventricular and graft lumina were observed, although the graft area never formed an integrated unit with the wounded ventricular wall. The peak of mitotic activity (3.19%) and thymidine labeling (28.1%) of graft cells, including many cells which resembled cardiac myocytes, was observed at 20 days. At 30 days, the graft was observed as a continuous wall composed primarily of muscle fibers. Several 30-, 50- and 70-day grafts had rhythmic contractions. These results suggest that amphibian cardiac muscle has histogenetic and proliferative capacities not attributable to mammalian cardiac muscle.

Notes: An ultrastructural comparison of mouse oocytes isolated at various stages of growth and meiotic competence has been carried out. Progressive changes in the nucleoli, ribosomes, mitochondria, endoplasmic reticulum, Golgi complex, and other organelles and inclusions of the oocyte have been examined as a function of oocyte size by transmission electron microscopy. The observations presented support the idea that growth of the mammalian oocyte involves not just tremendous enlargement of the cell, but extensive alterations in its overall metabolism as reflected in the ultrastructure of the oocyte at various stages of growth.

Notes: The mammalian ovary has been studied by optical microscopy and by scanning and transmission electron microscopy with the purpose of presenting an integrated view of the differentiating mammalian follicle. During follicular development, changes in the granulosa cells are particularly noteworthy and include dramatic modifications in cell shape coincident with antrum formation. The cytoplasmic processes of those granulosa cells immediately surrounding the oocyte, as well as the more peripheral granulosa cells comprising a second and third layer, traverse the zona pellucida, infrequently interdigitate with the microvilli of the egg, and make both desmosomal and gap junction contacts with the oocyte. The zona pellucida is thus distinguished by numerous fenestrations of varying diameters. The membrana limitans (basal lamina) is a bipartite structure composed of (a) a homogeneous stratum upon which the peripheral layer of granulosa cells rests, and (b) an outer region of collagen-like fibers. The specific advantages and limitations of the different methodologies utilized to study folliculo-genesis are discussed.

Notes: The gross external morphology of the salivary glands of Gromphadorhina portentosa is described from light, scanning, and transmission electron microscopic observations. Various techniques, such as cryofracturing and epoxy-fracturing followed by plastic removal, were employed. Internally, the transportation system is characterized by a cuticle-lined lumen bordered by duct cells. The duct collects secretory products, some of which are reabsorbed by duct cells. Products are transported to intercalary ducts and eventually to the hypopharynx and/or salivary reservoirs. Transmission electron micrographs demonstrate distinctive morphological differences between duct cells bordering ductules and those which line expanded regions of the duct. Duct cells which surround ductules have a microvillous-lined apical border in which the cuticular coat of the lumen may be only partially developed. Duct cells in other regions may retain microvilli, or the apical plasma membrane may invaginate and vesiculate. In some cells the apical region has neither microvilli nor invaginations, but possesses two morphologically different forms of microtubules. Some duct cells are characterized by the presence of lamellar bodies in the nuclear region and/or collagenous material above the basal lamina in the area where the acinar duct becomes confluent with the intercalary duct. The plasma membranes between adjacent duct cells within acini become convoluted, forming loops filled with cytoplasm. These loops, along with contact and septate desmosomes formed between membranes, may serve dual functions: adherent mechanisms between cells and/or transportation of materials between cells.

Notes: Fertilization in Notophthalmus viridescens is internal and involves passage of the sperm through five layers of egg jelly (J5-J1, from outermost to innermost), each of which is secreted by a discrete region of the oviduct. Polyspermy is normal. Passage of the sperm through the jelly and into the egg was studied by a technique of artificial insemination similar to natural insemination, in that undiluted fluid from the vas deferens was applied directly to eggs with various layers of jelly present, followed by flooding with water three to five minutes later. In general, successful fertilization increased as the number of jelly layers increased; jellyless coelomic eggs were not fertilizable. Sperm passage through the jelly and into the egg usually occurs within one to three minutes. Upon hydration of the jelly, barriers to sperm penetration develop in layers J5 and J3. Changes in the egg jelly thus seem to be involved in the restriction of polyspermy to a low level.

Notes: The gross and microscopic anatomy of the venom producing parotoid glands of Bufo alvarius has been studied by light and electron microscopy. Histochemical reactions for the presence of venom constituents and of components in biochemical pathways in the synthesis and release of venom were performed. The gland is composed of numerous lobules. Each lobule is an individual unit with a lumen surrounded by a double cell layer. Microvilli of the outer layer interdigitate with microvilli of the inner layer. Cells of the outer layer resemble smooth muscle cells, are rich in adenosine triphosphatase and glucose6-phosphatase, and contain numerous pinocytotic vesicles, glycogen granules and various organelles, These organelles include “crystalloids” of what seem to be highly organized agranular reticulum. These outer layer cells probably function in some aspects of venom synthesis, active cellular transport and contraction in the discharge of the secretory product. The inner cell layer demonstrates a positive chromaffin reaction, contains steroid material, various organelles, some pinocytotic vesicles and glycogen granules, and appears devoid of a plasmalemma on its inner surface. This layer is probably involved in venom formation and release via an apocrine type of secretion.Bufo alvarius parotoid gland shows significant morphological and histochemical differences from that of B. marinus and more nearly resembles a typical steroid producing organ.

Notes: Small local wounds on the surface of the mouse lung, produced by cauterization, healed by a typical reparative process involving c1 migration and increased cell division in alveolar and bronchial tissues. The local cell division response closely resembled the compensatory cell division response in the same organ which follows unilateral pnemnonectomy or unilateral collapse of the lung: initially there was an increase in the rate of DNA synthesis followed by an increased rate of entry into mitosis, both of these functions returning to normal levels within a few days. It is therefore suggested that both types of response are governed by a single regulatory mechanism. The results do not support the view that the rate of cell division is regulated by systemically-circulating mitotic control factors and it is proposed that changes in the cell division rate, both in the reparative and in the compensatory types of response, are determined by local alterations ill the concentration of regulatory metabolites.The magnitude of the cell division response was much greater in bronchial than in alveolar tissue. a result which is consistent with the view that new alveolar tissue may be produced by the proliferation and differentiation of bronchial cells.

Notes: Larvae of the stolidobranch ascidian Metandrocarpa taylori molt a thin sheath upon settling, then metamorphose and radiate a larval complement of vascular ampullae upon the substrate. These ampullae thereafter regress, “rest” in a reduced condition for several weeks, and then regrow into the oozooids definitive array of vascular ampullae in accompaniment to the development of the oozooidal vascular nest of test-vessels. Pallial buds emerge some four months after the larva settles; the oozooid has by then grown to a length of at least 2 mm and its vascular nest is surrounded by at least 16 vascular ampullae. Oozooids bud one to five buds (mean, 2.6) in a rather short period of blastogenic vigor, then persist in the colony. Late buds are frequently aborted. Buds appear anywhere around the basal margin of the oozooid, but more often on the left than the right and more often posteriorly than anteriorly. As other studies have observed with blastozooids, this study notes an integration of budding and the disposition of the elements of the test-vessel system of oozooids. Buds emerge oriented tangentially to the parental basal margin at the bud-site, then often rotate to point their anterior ends away from the parent. No larvae metamorphosed into oozooids with situs inuersus uiscerurn, but in this study two oozooids extruded blastozooids showing this anomaly; these blastozooids budded reversed zooids in turn, so that entire clonal lines showed the anomaly.

Notes: Guinea pig soleus, medial gastrocnemius and vastus lateralis muscles were compared for spindle density and distribution, number of intrafusal fibers per spindle and histochemical appearance of the axial bundle. A total of 326 spindles was used in the comparisons. Spindle density was over four times greater in the soleus than in either the medial gastrocnemius or vastus lateralis. In the soleus the spindles were distributed at random, but in the other two muscles no spindles were found in those fascicles in which fast-twitch glycolytic extrafusal fibers predominated. The average number of intrafusal fibers per spindle varied by less than 5% between the three kinds of muscles. About 80% of all spindles located had four intrafusal fibers, two of the nuclear bag type and two of the nuclear chain type. The histochemical appearance of the axial bundle was the same in each kind of muscle. Based on intensities of the myofibrillar adenosine triphosphatase reaction product at polar regions nuclear bag fibers were separable into two histochemical groups; nuclear chain fibers were of only one histochemical type.

Notes: To establish a morphological baseline for experimental studies of differentiation using the cement gland as a model, the following observations are added to those on record. The elongated cells of Xenopus laevis cement glands have an internal organization displaying five distinct zones differing in structure and specialized function. The apical zone contains packed secretion vesicles apparently belonging to two different types. The transit zone appears to be devoid of major biosynthetic activity and contains secretion vesicles migrating toward the surface. The zone of biosynthesis is typically organized in concentric regions. The very elongated nucleus lies in the next zone. Finally, the storage zone is characterized by lipid droplets and yolk platelets.Only quantitative differences are observed between cells of young and mature cement glands. Though all cells have the same general organization they may probably be divided into two subtypes according to the structure of their cytoplasm. The epithelial cells surrounding the gland differ according to their position along lateral or basal borders.

Notes: The fine structure of the mature sperm of the holothurian, Cucumaria miniata, and the ophiuroid, Ophiopholis aculeata, is described with particular reference to their acrosomal and centriolar satellite complexes, and compared to the sperm of other echinoderms.In Cucumaria, the acrosome is in the form of a diffuse acrosomal vesicle. It is unusual in that it apparently lacks an acrosomal membrane. A membrane separating the acrosomal vesicle from the periacrosomal material may not be equivalent to a typical inner acrosomal membrane. In Ophiopholis, the acrosome is dense, with some internal substructure, and is enclosed by a complete acrosomal membrane. In both species, the acrosome is partially surrounded by an amorphous periacrosomal mass. There is a notable absence of a subacrosomal depression and associated structures as found in other echinoderm sperm.The centriolar satellite complex (CSC) is essentially identical in both species. A reconstruction of the CSC is presented. The CSC consists of nine satellites radiating angularly from the distal centriole, each bifurcating at a dense node before inserting on a marginal ring containing circumferential microtubules. The ring is probably a cytoskeletal element. Immediately below the satellites are nine Y-shaped connectives. connecting each of the axonemal alpha doublets to the flagellar membrane.

Notes: The fine structure of the dorsal bristle complex and pellicle of non-developing Euplotes eurystomus is described in detail by scanning and transmission electron microscopy. The bristle-pit unit is a highly differentiated complex of organelles. The bristle complex is composed of a pair of kinetosomes (basal bodies) joined by a connective. The anterior kinetosome bears the bristle cilium, which contains a polarized network of particles (“lasiosomes”). The posterior kinetosome bears a very short, knob-like “condylocilium,” and has an associated striated fiber. Accessory ribbons of microtubules are also associated with the kinetosome couplets. Parasomal sacs, a septum connecting the bristle cilium to the anterior wall of the pit, core granules of the kinetosomes, and large membranous ampules are described. The organization of the bristle complex bears many similarities to the somatic ciliature of other ciliates. The pellicle of Euplotes is composed of a continucus outer cell membrane subtended by membranous alveoli, which contain a “fibrous mat.” Two sheets of subpellicular microtubules (longitudinal and transverse) are located just beneath the alveoli. The “epiplasm” seen in some other ciliates is apparently absent in Euplotes. The texture of the cell surface is a pattern of folds or rugae composed of the outer cell membrane and the upper membrane of the alveolus. The pattern of rugae probably defines the “silverline-system” of light microscopy.

Notes: Hypotrichs are among the most complex ciliates in terms of morphology and development. To study the fine structure of cortical morphogenesis associated with cell division in Euplotes eurystomus, three different methods of observation were employed: light microscopy of protargol-stained specimens, scanning electron microscopy of cells prepared by critical point drying, and transmission electron microscopy of sectioned material. Observations on the stages of morphogenesis give much new information about cortical development, particularly about proliferation and aggregation of kinetosomes (basal bodies), ciliary outgrowth, the topography of morphogenesis, cirrus resorption, and growth of the pellicle. During the formation of new cirrus the process of kinetosome proliferation is atypical, i.e., groups of prokinetosomes are seen oriented at random and, in some cases, prokinetosomes apparently are formed at a distance from nearby young kinetosomes. That the new cirri develop in surface grooves, the grooves elongate into “tracks,” and (in some cases) grooves are partitioned into separate tracks suggests that the grooves play a role in the orderly migration of the new cirri on the cell surface. Conspicuous morphogcnctic changes in the cell surface involve local growth of the pellicle. The process of pellicle growth apparently involves two basic steps: (a) growth of the outer cell membrane to form “bare regions,” and (b) formation of alveoli in the bare regions. Alveolar sheets are formed by fusion of alveolus precursor particles. Cirrus resorption is sequential over several stages of development, and old cirri are resorbed as the new cirri impinge on them. As the old cirri regress, both in situ resorption and retraction of axonemes into the cytoplasm occur.

Notes: The mature annelid cuticle contains orthogonally oriented collagen in a matrix capped superficially by a dense epicuticle with external corpuscles. The underlying epidermis is a simple columnar epithelium with two major cell types, mucous-secreting cells which secrete through channels in the cuticle to the exterior of the worm, and “supportive” cells which presumably produce and increase the cuticle by secreting into it.The structures of supportive cells, previously interpreted as specialized for establishing interfibrillar collagen order, are revealed by glutaraldehyde fixation as common cellular components without the qualities deemed useful to align collagen. Cell processes which penetrate and sometimes pass completely through the cuticle are not stable, not in geometric order, and lack cilia-like structure. Cilia, unlike the ubiquitous cellular processes, are highly restricted to regions of the epidermis with specialized functions. Cellular control, or other control, of collagen fibrillogenesis remains unestablished.

Notes: Early embryogenesis is described for the southern corn rootworm, Diabrotica undecimpunctata Howardi Barber, at 24 ± 1°C. During the first four hours following oviposition, the maturation divisions and syngamy are completed. Morphological changes in the second polar body accompany syngamy. Cleavage divisions and energid migration occur during the fourth to the tenth hour. The vitellophags, which appear during cleavage divisions, are distinguished from the blastema-bound nuclei by having smaller, more densely staining nuclei. After completion of a uniform blastoderm (11-14 hour), cell division ceases until the completion of the germ band and the formation of the embryonic membranes (22 hour). This species has a pattern of amnion formation that is different from most Coleoptera but is shared with a few other chrysomelids, some Isoptera, and some Odonata.

Notes: Each ocellar nerve in the house cricket Acheta domesticus contains giant nerve fibers of 10-15 μ diameter, characterized in Golgi Cox preparations by a single row of short collaterals which runs along nearly the entire length of a fiber. Numerous long collaterals are given off by thin fibers in the ocellar nerve; medium-size fibers give off relatively few collaterals.The lateral ocellar tracts extend posteriorly through the dorsal protocerebrum, crossing the protocerebral bridge dorsally. The smaller median ocellar tract runs more ventrally through the pars intercerebralis; posterior to the bridge its fibers turn out toward the lateral nerves. Golgi and cobalt preparations reveal branching of giant and mediu_-size ocellar fibers posterior to the bridge at two levels, forming bilateral regions of ocellar neuropile. No ocellar processes appear to be given off to the corpora pedunculata, centra! body, nervi corporis cardiaci, antenna! lobes, or circumesophageal connectives; it is uncertain whether ocellar collaterals extend into the protocerebral bridge or optic lobes. Cell bodies of giant and medium-sized fibers are located in the pars intercerebralis.

Notes: The general morphology of the gills is similar in larval (ammocoetes) and parasitic adult sea lampreys, Petromyzon marinus, despite different methods of ventilation necessitated by their feeding habits.The gill lamellae are supported by randomly-distributed pillar cells which enclose blood spaces and collagen columns. The distribution of these cells in lampreys is different from that of higher fishes and it may be inefficient for respiratory exchange. The presence of cytoplasmic microfilaments suggests that these cells have the ability to reduce the lamellar blood spaces through contraction. Marginal channels at the tips of the lamellae are lined only by endothelial cells.The thickness of the water-blood pathway in lampreys falls within the range described for higher fishes, with the most efficient gas exchange likely occurring at the lamellar tips where only a single layer of epithelial cells is present. The abrupt increase in height of the epithelium near the lamellar bases in adults, compared to the gradual transition in height along the lamellae in ammocoetes, is perhaps reflective of higher oxygen requirements during the parasitic stage. The consistent appearance of wide, lateral intercellular spaces within the respiratory epithelium of lampreys indicates possible involvement of these spaces in transport.Mucous secretion appears to be an important function of the superficial platelet cells in ammocoetes. “Mitochondria-rich” and “mitochondria-poor” superficial cells are observed in both ammocoetes and adults, with the mitochondria-rich cells more prevalent toward the lamellar bases. The possibility that at least some of these cells may be involved in absorption is discussed. Mitochondria-rich cells in the interlamellar region are morphologically different in ammocoetes and adults but all possess an abundance of smooth endoplasmic reticulum and hence resemble “chloride cells” of higher fishes. The similarity of these cells in the parasitic adult lamprey to chloride cells of marine fishes may reflect the potential of the adult lamprey to osmoregulate in salt water. A scarcity of these cells in ammocoetes and their resemblance to chloride cells in freshwater fishes may reflect the restriction of larval lampreys to a freshwater habitat.

Notes: Sexual dimorphism in the distribution of antennal sense organs is common among adults of the genus Periplaneta. In three out of the four strains of Periplaneta americana examined, adult males had more contact chemoreceptors than females. In the fourth strain of P. americana and in P. australasiae, P. brunnea, P. fuliginosa, and P. japonica, no statistically supportable sexual dimorphism of contact chemoreceptors was found. However, in all strains and species of Periplaneta examined, sexual dimorphism was found in the total number and/or density of olfactory sensilla. Male adults had nearly twice as many olfactory sensilla as female adults. These observations are consistent with the behavioral observation that males within the genus Periplaneta rely on the reception of an airborne pheromone for the initiation of courtship behavior. In P. americana, where sexual dimorphism was found in the contact chemoreceptors, contact stimuli release the full wing raising display and presentation in males during courtship.

Notes: The main excretory ducts (MED's) from the submandibular gland of adult cats were examined by electron microscopy. The ducts consisted of a pseudostratified epithelial lining surrounded by abundant connective tissue and numerous, small, longitudinally-oriented blood vessels. The taller epithelial cells were closely coherent, without the luminal clefts between adjacent cells that are characteristic of rat MED's. In the cat, these cells lacked basal membrane specialization, but showed considerable lateral interdigitation. Some microvilli were present on the apical surface. In a'few rare cells, the luminal surface bore cilia of typical appearance. The smaller, pyramidal basal cells had irregular basal surfaces that gave rise to one or more long cytoplasmic processes. The basal surface of the pyramidal cells was studded with hemidesmosomes. The cytoplasm contained abundant tonofilaments, which sometimes aggregated in prominent perinuclear bundles. Occasional goblet cells were present in the duct wall.MED's perfused either in situ or in a perfusion chamber with Locke's solution also were studied. Even after perfusion of 160 minutes duration, the ultrastructure of the ductal epithelium showed remarkably few alterations. The MED model system thus remains stable long enough to carry out physiological experiments which may produce ultrastructural alterations.

Notes: The byssus attachment plaque and the tissues responsible for its formation were studied in M. califomianus by light microscopy and by transmission and scanning electron microscopy. It was shown that the plaque consists of at least three phases which ultrastructurally resemble three secretions considered to be collagen, mucoid material and polyphenol. The mucoid and polyphenol appear to mix as a colloidal suspension in which the latter is the continuous phase and forms the definitive bonding surface. Plaque collagen represents an extension of thread material into the cementing substance.Stimulated secretion within the ducts and distal depression of the mussel's foot shows a continuum of increasing heterogeneity from the inner toward the outer regions. This reflects the distribution of exocrine cell apices wherein exocytosis of polyphenol granules predominate deeply, mucous granules superficially and collagen granules in between.It is proposed that the morphology of the plaque conforms to theoretical physical-chemical requirements for adhesion under water.

Notes: In this paper the cranial arteries, cranial arterial foramina, and bony canals of the Cheloniidae, Chelydridae, Pelomedusidae, and Chelidae are described in detail. From skull studies and published material, the general cranial arterial patterns of all the turtle families can be inferred. Sea turtles, the Cheloniidae and Dermochelyidae, possess both a large stapedial artery and a large artery supplying the orbit, which is possibly similar to the primitive cranial arterial pattern for turtles. From a primitive pattern in which stapedial and palatine arteries supply the orbit, the Chelydridae and Testudinidae retained a large stapedial artery and reduced the palatine artery, while the Kinosternidae and Dermatemydidae developed a large palatine artery and reduced the stapedial artery. The Trionychidae and probably the Carettochelyidae evolved a complex arterial pattern in which the stapedial artery was reduced somewhat and the pseudopalatine artery was substituted for the palatine artery. Pleurodires in general retained a large stapedial artery and reduced or eliminated the palatine artery. The Podocneminae, including the Madagascar species, developed a highly modified carotid canal, which is found in no other turtle group. The facts which have been presented should aid in fossil skull studies and in understanding the evolutionary background of recent turtles.

Notes: Ethidium bromide (l0 μg/ml) and bromodeoxyuridine (25 μg/ml) were used to sensitize selective cell organelles to visible wavelengths of an argon ion Her (488 and 514 nanometers). Ethidium bromide was shown to be salabtlve In sensitizing nucleoli, chromosomes, and the centriolar region of PTK2 cells to the laser microbeam. Similarly, BrDU sensitized chromosomes to the microbeam irradiation. The lesions produced on the chromosomes when either agent was used appeared as a phase paling of the irradiated segment. Nucleolar lesions also appeared as a phase paling, and the centriolar region alteration appeared either as a phase paling or a phase darkening.