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  • Histochemistry  (23)
  • Chromatographie, Gas  (18)
  • Springer  (41)
  • American Geophysical Union
  • Irkutsk : Ross. Akad. Nauk, Sibirskoe Otd., Inst. Zemnoj Kory
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • Wiley
  • 2015-2019
  • 2005-2009
  • 1975-1979  (14)
  • 1970-1974  (27)
  • 1975  (14)
  • 1973  (27)
Collection
Keywords
Publisher
  • Springer  (41)
  • American Geophysical Union
  • Irkutsk : Ross. Akad. Nauk, Sibirskoe Otd., Inst. Zemnoj Kory
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • Wiley
Years
  • 2015-2019
  • 2005-2009
  • 1975-1979  (14)
  • 1970-1974  (27)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Comparative histochemical and electron microscopic studies of the sinus and venous walls of the human spleen with special reference to the sinus-venous connections (1975)
    Springer
    Cell & tissue research 163 (1975), S. 519-533 
    Details
    ISSN: 1432-0878
    Keywords: Human spleen ; Sinus lining cells ; Pulp veins ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sinus and venous walls of normal human spleens were studied with enzyme histochemical and electron microscopic methods. Particular attention was paid to the connections between sinuses and veins. Histochemically the sinus lining cells revealed a distinct naphthol-AS-acetate-esterase activity but no reaction for alkaline phosphatase. Venous endothelial cells were positive for the latter but negative for the former enzyme. In the sinusvenous junctional area there were no endothelial cells with reactivity for both enzymes. Electron microscopically both the sinus lining cells and the venous endothelial cells could be clearly characterized and therefore easily distinguished from one another on morphological grounds. There were no clear ultrastructural indications of transitional forms between sinus lining cells and venous endothelial cells in the sinus-venous area. According to these findings, sinus lining cells represent a specialized endothelium, but one with practically no morpholgical similarities to the venous endothelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218497
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructure and fluorescence histochemistry of endocrine (APUD-type) cells in tracheal mucosa of human and various animal species (1975)
    Springer
    Cell & tissue research 158 (1975), S. 425-437 
    Details
    ISSN: 1432-0878
    Keywords: Tracheal epithelium (human, animal) ; APUD-Endocrine system ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study describes distinctive cells with ultrastructural and histochemical features of APUD-type endocrine cells within the tracheal epithelium of human fetuses, newborns and children as well as different animal species. These cells referred to as Kultschitzky cells (K cells) were found to be argyrophilic, but not argentaffin, and are considered analogous to the same type of cells in lung and gastro-intestinal tract. Fluorescence histochemistry demonstrated the presence of intracellular amine within tracheal K cells, but only after in-vitro or in-vivo administration of amine precursor (L-DOPA). Ultrastructurally, these cells are characterized by the presence of numerous cytoplasmic granules (dense core vesicles) which show species related morphologic variations. Two different types of K cells were found in trachea of lamb and armadillo, each type possessing morphologically different dense core vesicles. In human and rabbit tracheas, only one type of K cell was identified. K cells in the trachea are distributed as single cells between other epithelial cells; neuroepithelial bodies such as those found in bronchial mucosa were not identified. Well differentiated K cells were found in tracheas of early human fetuses and throughout gestation, infancy, and childhood. Preservation of K cells in human autopsy material and widespread occurrence of these cells in various laboratory animals will permit further studies into the nature and function of tracheobronchial endocrine cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220210
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  • 3
    Electronic Resource
    Electronic Resource
    Histochemical studies of the adrenocortical homologue in the kidney of the Australian lungfish, Neoceratodus forsteri (1975)
    Springer
    Cell & tissue research 156 (1975), S. 533-538 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Neoceratodus (Dipnoi) ; 3βol-steroid dehydrogenase ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A histochemical study has been made of groups of cells lying adjacent to the blood vessels of the kidney of Neoceratodus forsteri. The cells contain lipid droplets of varying sizes, stain positively for cholesterol and its esters and contain 3βol-steroid dehydrogenase activity. Similar cells have previously been described in another dipnoan fish, Protopterus, and in a urodele, Pleurodeles waltlii and are considered to be the adrenocortical tissue of these species. Because of the histochemical characteristics of the cells together with their location, we conclude that they represent the adrenocortical homologue of N. forsteri. As is the case in Protopterus, no chromaffin tissue was detected in the kidney of N. forsteri.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225111
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  • 4
    Electronic Resource
    Electronic Resource
    Observations on the fine structure, enzyme histochemistry, and innervation of parathyroid gland and ultimobranchial body of Chthonerpeton indistinctum (Gymnophiona, Amphibia) (1975)
    Springer
    Cell & tissue research 164 (1975), S. 105-119 
    Details
    ISSN: 1432-0878
    Keywords: Ultimobranchial body ; Parathyroid gland ; Amphibia ; Gymnophiona ; Innervation ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fine structural and enzyme histochemical observations on ultimobranchial body and parathyroid gland of the caecilian Chthonerpeton are presented. The cell clusters and follicles of the ultimobranchial body consist mainly of granulated cells which are termed C-cells and obviously belong to the APUD cell series. In the larger follicles additional possibly exhausted degranulated cells and replacement cells occur. A rich supply of nerve fibres has been found in this gland. Frequently nerve terminals were observed to come into synaptic contact with the C-cells. Two categories of nerve fibres occur: a) fibres containing large polymorphic electron dense granules (probably purinergic fibres), b) fibres containing small electron transparent vesicles and a few electron dense granules (probably cholinergic fibres). The parathyroid gland consists of elongated cells (one cell type) poor in organelles and often containing fields of glycogen and lipid droplets. The cells are further characterized by fair amounts of lysosomal enzymes; they are interconnected by maculae adhaerentes and occludentes. No nerves and blood vessels have been found in the parathyroid gland of Chthonerpeton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221698
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  • 5
    Electronic Resource
    Electronic Resource
    A monoamine in the gustatory cell of the frog's taste organ (1975)
    Springer
    Cell & tissue research 159 (1975), S. 101-108 
    Details
    ISSN: 1432-0878
    Keywords: Taste organ ; Frog ; Monoamine ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fluorescence histochemistry reveals that in the frog's taste organ a yellow fluorescence is regularly observed at the most basal region of the sensory epithelium. The fluorescence has a strong intensity, but it fades rapidly upon the UV-irradiation. The peak of the emission spectrum is at 520 mμ. Following reserpine treatment the yellow fluorescence is markedly reduced, but not depleted completely. From these characteristics the monoamine fluorescence is regarded as representing 5-HT (serotonin). The ultrastructural study on sensory epithelia shows that the terminal portions of gustatory cell processes are localized at the basal region. These portions are filled with dense cored vesicles (700–1000 Å in diameter) and frequently opposed with nerve fibers penetrating into the epithelium. The gustatory cell processes are also interposed between the terminal portions or nerve fibers. The cytoplasm of the gustatory cell process is characterized by many mitochondria, fine filaments and glycogen particles, but contains few cored vesicles. The distribution of terminal portions of gustatory cell processes seems to correspond fairly well to that of the monoamine fluorescence observed discontinuously along the basal lamina. Accordingly it is concluded that the fluorigenic monoamine is localized in the cored vesicles of the gustatory cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231999
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  • 6
    Electronic Resource
    Electronic Resource
    Pantolacton aus Coenzym A. Bestimmung von Pantothensäure (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 277 (1975), S. 124-124 
    Details
    ISSN: 1618-2650
    Keywords: Pantothensäure, Pantolacton ; Chromatographie, Gas ; Hydrolyse von Coenzym A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00602082
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  • 7
    Electronic Resource
    Electronic Resource
    Ermittlung des Vernetzungsgrades und Beladungszustandes von Ionenaustauschern auf Kunstharzbasis durch Pyrolyse-Gas-Chromatographie (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 277 (1975), S. 9-17 
    Details
    ISSN: 1618-2650
    Keywords: Ermittlung des Vernetzungsgrades, Beladungszustandes von Ionenaustauschern ; Chromatographie, Gas ; Pyrolyse, Kunstharzbasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Zur besseren Reproduzierbarkeit der Zusammensetzung des Pyrolysates bei der Pyrolyse-Gas-Chromatographie wird eine spezielle Eingabenanordnung mit Trägergasumschaltung beschrieben und deren Einfluß auf die Bildung der Pyrolyseprodukte und auf die Retentionszeiten mit einer Modellsubstanz untersucht. Mit Hilfe der Trägergasumschaltung kann man die Bandenflächenverhältnisse zur Bestimmung des Vernetzungsgrades von Ionenaustauschern auf Styrol-DVB-Basis wesentlich genauer ermitteln. Da bei der Pyrolyse von Anionenaustauschern wenigerα-MSt auftritt als bei den Kationenaustauschern, sind die Bandenflächenverhältnisse bei ersteren wesentlich größer. Bei den Anionenaustauschern werden sie mit steigendem Vernetzungsgrad kleiner, wohingegen bei den Kationenaustauschern die Werte steigen. Bei 400° C erfolgt der thermische Angriff im wesentlichen an der aktiven Gruppe der Anionenaustauscher mit Styrol-DVB-Matrix. Die aus dem Gegenion entstehenden Methylester bzw. deren Zersetzungsprodukte sind charakteristische Pyrolyseprodukte. Kennzeichnend für Dowex 1 ist das Auftreten von Trimethylamin, für Dowex 2 das von Acetaldehyd. Somit ist es mit der Pyrolyse-Gas-Chromatographie möglich, zusätzlich zur Matrix, Art und Struktur der funktionellen Gruppe sowie Substitutionsstelle der Ankergruppen an der Matrix auch Vernetzungsgrad und Beladungszustand von Ionenaustauschern zu ermitteln.
    Notes: Abstract A special introduction device with switching of carrier gas for improving the reproducibility of pyrolysate composition in pyrolysis gas-chromatography has been described. Its influence on the formation of pyrolysis products and on retention times has been studied on a model substance. The peak area ratios for determining the degree of cross-linking in ion exchangers on styrene-DVB basis can be estimated considerably more accurately with the help of carrier gas switching. Since anion exchangers yield lessα-methylstyrene on pyrolysis than do cation exchangers, their peak area ratios are appreciably larger. Increasing the degree of cross-linking lowers the ratios with the anion exchangers and raises them with the cation exchangers. At 400° C the thermal attack on anion exchangers with styrene-DVB matrix occurs mainly at the active group. The methyl esters or their decomposition products formed from the counter ions are characteristic pyrolysis products. A feature of Dowex 1 is the formation of trimethylamine; of Dowex 2, acetaldehyde. In this way, pyrolysis gas-chromatography enables information about the degree of cross linking and the nature of the anion of ion exchangers, as well as about the matrix, type and structure of the functional group and position of substitution of the anchor group in the matrix to be obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00591870
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrapolare Mischphasen zur GLC-Bestimmung von Benzol (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 277 (1975), S. 31-32 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Benzol ; Chromatographie, Gas ; ultrapolare Mischphasen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00591874
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  • 9
    Electronic Resource
    Electronic Resource
    Zur Analytik von Organozinnstabilisatoren (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 273 (1975), S. 19-23 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von Organozinnverbindungen, Alkylzinnverbindungen, Organozinnstabilisatoren ; Chromatographie, Gas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die quantitative Bestimmung von Mono-, Di-, Tri- und Tetraalkylzinnverbindungen in Mischungen durch Gas-Chromatographie ist möglich nach Alkylierung zu gemischten Tetraalkylzinnderivaten. Die Alkylierung mit Methyl-, Butyl- und Octylmagnesylchlorid in Diäthyläther ist quantitativ und verläuft ohne Veränderung der ursprünglichen Zinnverteilung. Sie läßt sich nicht nur auf Alkylzinnoxide und -chloride, sondern auch direkt auf Alkylzinn-thioglykolate anwenden. Die aus der Umsetzung der Thioglykolsäureester resultierenden Nebenprodukte werden von der gas-chromatographischen Analyse an Kieselgel absorbiert und die Tetraalkylzinnverbindungen mit n-Pentan eluiert. Die gas-chromatographische Trennung wird wahlweise an Carbowax oder Polyglycol 20000 durchgeführt bei Temperaturen zwischen 100 und 230° C. Die Peak-Flächen entsprechen den Gew.-% Tetraalkylzinn.
    Notes: Abstract The quantitative determination of mono-, di-, tri- and tetraalkyl tin compounds in mixture by GLC is possible after alkylation to mixed tetraalkyl tins. The alkylation with methyl-, butyl- and octyl magnesium chloride runs quantitatively without any change in the original alkyl groups attached to the tin. The method can be applied not only for alkyl tin oxides and chlorides but also directly for alkyl tin thioglycolates. The by-products resulting from the thioglycolic acid ester cleavage are absorbed on silica gel prior to GLC and the tetraalkyl tins are eluted with n-pentane. Gas-chromatographic analysis is carried out alternatively with carbowax and polyglycol 20000 at temperatures between 100 and 230° C. The peak areas correspond to the weight percentage of tetraalkyl tin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00459399
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  • 10
    Electronic Resource
    Electronic Resource
    Gas-chromatographische Überwachung des Extraktionsmittels bei der Wiederaufarbeitung von HTR-Brennelementen (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 273 (1975), S. 113-116 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Monobutylphosphat, Dibutylphosphat in Tributylphosphat, n-Alkanen ; Chromatographie, Gas ; Überwachung der Wiederaufarbeitung von HTR-Brennelementen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Bei der Wiederaufarbeitung von HTR-Brennelementen durch Solventextraktion nach dem THOREX-Prozeß wird das als Extraktionsmittel verwendete Tributylphosphat/n-Alkan-Gemisch einer intensiven hydrolytischen und radiolytischen Beanspruchung ausgesetzt. Hauptprodukte sind Di- und Monobutylphosphat, die schon in geringen Konzentrationen durch Bildung nicht rückextrahierbarer Urankomplexe und Überführung gewisser Spaltprodukte in die wäßrige Produktphase zu Störungen bei der Extraktion führen. Beide Ester werden durch Sodawäsche aus dem bestrahlten Extraktionsmittel entfernt, welches danach wiederverwendet werden kann. Zur Kontrolle der Wirksamkeit dieser Solventwäsche, der Zusammensetzung des frischen und der Gesamtqualität des recyclierten Extraktionsmittels soll die Gas-Chromatographie innerhalb des JUPITER-Prozesses eingesetzt werden. Sie erlaubt eine quantitative Bestimmung von DBP und MBP bis zu Gehalten von etwa 25 ppm. Die Analysendauer beträgt ca. 100 min.
    Notes: Abstract During reprocessing of HTR fuel elements by the THOREX solvent extraction process the tributylphosphate/n-alcane mixture used as extractant is subject to an intensive radiolytic and hydrolytic burden. Main degradation products are di- and monobutylphosphate which in quite low concentrations disturb the solvent extraction by retention of uranium and transfer of some fission products into the aqueous product solution. Both esters are removed from the irradiated solvent by washing with a sodium carbonate solution, after which the solvent may be recycled. For a control of the efficiency of this solvent recovery procedure as well as of the composition of the fresh and recycled solvent in the JUPITER reprocessing facility, gas chromatography will be used. This method allows the determination of di- and monobutylphosphate down to concentration levels of about 25 parts per million. An analysis requires about 100 min.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00426269
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  • 11
    Electronic Resource
    Electronic Resource
    Gas chromatography of chlorides and bromides of III-, IV- and V-group elements in the periodic table (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 274 (1975), S. 353-357 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von Chloriden, Bromiden ; Chromatographie, Gas ; Elemente der III. -V. Gruppe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The theory for the relation between retention volume and boiling point in gas chromatography was applied to the retention volumes for bromides of IV- and chlorides and bromides of III- and V-group elements in the periodic table, as an extension of the previous paper on chlorides of IV-group elements. The apparent solubility parameter of solventβδ S was separated into the non-regularity parameterβ and the solubility parameter of solventδ S, and the meaning ofβ and the value ofδ S were estimated empirically. Followingδ S values were obtained: Silicon oil DC 550 (8.1), Apiezon grease L (9.1), PEG 1500 (11.6), FeCl3 · 6 H2O (12.5), Low melting point alloy (12.5) and Ga metal (13.4).
    Notes: Zusammenfassung Die Theorie über die Beziehung zwischen Retentionsvolumen und Siedepunkt in der Gas-Chromatographie wurde auf die Retentionsvolumina der Bromide der IV. sowie der Chloride und Bromide der III. und V. Gruppe angewendet (in Fortführung der früheren Arbeit über die Chloride der IV. Gruppe). Der scheinbare Löslichkeitsparameter des Lösungsmittels (βδ S) wurde aufgetrennt in den Unregelmäßigkeitsparameter (β) und den Löslichkeitsparameter des Lösungsmittels (δ S). Die Bedeutung vonβ und der Wert vonδ S wurden empirisch bestimmt. Die folgendenδ S-Werte wurden erhalten: Siliconöl DC 550 (8,1), Apiezonfett L (9,1), PEG 1500 (11,6), FeCl3 · 6 H2O (12,5), niedrigschmelzende Legierung (12,5), Ga-Metall (13,4).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00626033
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  • 12
    Electronic Resource
    Electronic Resource
    Gas-chromatographische Bestimmung von monosubstituierten Acetylenen in der C4-Fraktion (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 275 (1975), S. 27-27 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Acetylen, Methylacetylen, Äthylacetylen, Vinylacetylen ; Chromatographie, Gas ; Isolierung als Acetylide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00432994
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  • 13
    Electronic Resource
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    Notiz über eine gas-chromatographische Bestimmung von Essigsäure und Propionsäure in Polyamid 6 (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 276 (1975), S. 127-128 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Essigsäure und Propionsäure in Polyamid 6 ; Chromatographie, Gas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01165816
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  • 14
    Electronic Resource
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    3-Hydroxyglutarsäuredinitril als Trennflüssigkeit für die GLC (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 277 (1975), S. 373-373 
    Details
    ISSN: 1618-2650
    Keywords: Verw. von 3-Hydroxyglutarsäuredinitril als Trennflüssigkeit ; Chromatographie, Gas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423783
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  • 15
    Electronic Resource
    Electronic Resource
    A comparison of histological methods for demonstrating calcification (1973)
    Springer
    Calcified tissue international 12 (1973), S. 169-173 
    Details
    ISSN: 1432-0827
    Keywords: Calcification ; Histochemistry ; Autoradiography ; Fetal ; Mandible
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des rats femelles reçoivent du45Ca ou du32P au 13ème et 18ème jour de la grossesse. Les coupes sériées des têtes de foetus sont étudiées par autoradiographie et par diverses méthodes histologiques pour déterminer la calcification. La détection la plus précoce de45Ca s'observe simultanément comme une réaction positive pour le calcium avec une des méthodes histologiques utilisées.32P est en évidence par les méthodes autoradiographiques un peu plus tard que le45Ca et sa présence coincide avec la réaction positive la plus précoce observée avec les autres méthodes histologiques utilisées. Les os de ces têtes foetales commencent à se calcifier selon un mode particulier commençant par la mandibule, puis l'os frontal et le maxillaire supérieur, suivis par les os nasaux, pariétaux et interpariétaux.
    Abstract: Zusammenfassung Weibliche Ratten erhielten45Ca und32P zwischen dem 13.–18. Tag der Schwangerschaft. Zur Ermittlung der Verkalkung wurden Serienschnitte der Köpfe ihrer Feten mittels Autoradiographie sowie durch verschiedene histologische Methoden untersucht. Das erste Auftreten von45Ca war ebenfalls von einer positiven Calciumreaktion mittels einer der verwendeten histologischen Methoden begleitet.32P wurde in der Autoradiographie erst etwas später als45Ca festgestellt und dessen Nachweis deckte sich mit den ersten positiven Reaktonen aller anderen verwendeten histologischen Methoden. Die Knochen in diesen Fetusköpfen begannen in einer bestimmten Sequenz zu verkalken: zuerst der Unterkiefer, dann das Stirnbein und der Oberkiefer, dann das Nasenbein, die Parietal- und Interparietalknochen.
    Notes: Abstract Female rats were given45Ca or32P from 13 to 18 days of pegnancy. Serial sections from the heads of their fetuses were studied by autoradiography, as well as by several histological methods for assessing calcification. The earliest detection of45calcium occured at the same time as a positive reaction for calcium with one of the histological methods used.32P was not detectable by autoradiographic methods until somewhat later than45Ca, and its presence coincided with the earliest positive reaction with all of the other histological methods employed. The bones in these fetal heads began to calcify in a partcular sequence, the mandible first, then the frontal bone and maxilla, followed by the nasal, parietal and interparietal bones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013732
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  • 16
    Electronic Resource
    Electronic Resource
    A morphologic and histochemical study of biliary atresia in lamprey liver (1973)
    Springer
    Cell & tissue research 136 (1973), S. 85-96 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Lampetra ; Physiological biliary atresia ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A morphologic and histochemical study was carried out on the liver of larval and adult lampreys at the optical and electron microscopic level. In the larva the liver is composed of blind ending single cell thick tubules of hepatocytes. The tubular lumina provided with microvilli are morphologically comparable with the canalicular lumens of the higher species of animals. The cytoplasm of the hepatocytes contains numerous inclusions with heterogeneous appearance and crystalline material. The biliary system is composed of numerous bile ductules and ducts. In the adult lamprey, the biliary system has disappeared. The hepatocytes loose their tubular arrangement and the characteristic differentiation of their biliary pole. In contrast to previous reports in the literature the presence of bile pigment in the adult lamprey liver could not be demonstrated with any histochemical technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307681
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  • 17
    Electronic Resource
    Electronic Resource
    Histochemical studies on the morphology of the golgi apparatus and its relationship to catecholamine biosynthesis in the locus coeruleus of the rat (1973)
    Springer
    Cell & tissue research 136 (1973), S. 329-348 
    Details
    ISSN: 1432-0878
    Keywords: Locus coeruleus (Rat) ; Neurons ; Golgi apparatus ; Catecholamine biosynthesis ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Detailed histochemical studies have been performed on the morphology of the Golgi apparatus (GA) by application of the thiamine pyrophosphatase (TPPase) method (Novikoff and Goldfischer, 1961) to the neurons of the locus coeruleus (LC) of normal and catecholamine biosynthesis inhibitors (fusaric acid and D, L-α-methyl-p-tyrosine methylester HCl) given adult healthy male Wistar strain rats. The neurons were classified into five categories on the basis of the morphology of the Golgi apparatus. The number of cells in individual categories was counted to evaluate the percentage of each category in the whole nucleus. The majority of cells belongs to Types II, III, and IV whose GA goes through cyclic activity, but the remaining neurons belong to Types I and V which may have a strong tendency to be different from the former in character. The latter neurons correspond formally with Types I and V of the rabbit LC, but they do not respond to the drugs administered. The rat LC is very similar to the dorsal vagal nucleus of the rabbit in regard to the dominant category. The present results indicate that the majority of the rat LC neurons may work vigorously and they may be motor neurons. Administration of the drugs caused reduction of TPPase activity, augmentation of disintegration and the budding-off process of the GA of Type IV, a decrease in the percentage of Type IV and an increase in that of Type II. Administration of 100 mg/kg fusaric acid caused maximal morphological change of the GA at the 90th minute; however, administration of 200 mg/kg fusaric acid showed more marked change of the GA, having two peaks and two valleys. The GA revealed much more intense reaction to D,L-α-methyl-p-tyrosine methylester HCl than to fusaric acid. The present results indicate that tyrosine hydroxylase may be the rate-limiting enzyme in the catecholamine biosynthesis. These noticeable changes of GA caused by administration of the drugs were completely restricted to the neurons of LC and the neurons of the mesencephalic nucleus of the trigeminal nerve did not show any morphological changes of the GA. These results strongly suggest that the GA of the rat LC neurons may have ability to synthesize catecholamine whereas the GA of the rat mesencephalic nucleus of the trigeminal nerve may be completely devoid of this ability and that the role of the GA may be different depending on the anatomical regions.
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    Electron microscopic observations on the mucous secretory activity of the human gallbladder epithelium (1973)
    Springer
    Cell & tissue research 139 (1973), S. 463-471 
    Details
    ISSN: 1432-0878
    Keywords: Gallbladder (Human) ; Mucus secretion ; Reverse pinocytosis ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mucous secretory activity of the human gallbladder epithelium was investigated by light and electron microscopy and with histochemical techniques. There are two types of granules in the supranuclear region of the epithelial cells. The one low in density contains a fine filamentous material and gives a strongly positive silver methenamine reaction. The other is dense and only faintly positive. The granules of the former are considered to be mucous secretory granules and the granules of the latter may be lysosomes. PAS positive granules correspond presumably to both types of granules mentioned above. The mucous secretory granules are considered to be synthesized by the Golgi apparatus and the granular endoplasmic reticulum as has been confirmed in other mucous secretory cells. Their content is released from the cell by reverse pinocytosis. Typical goblet cells occur frequently in the fetal epithelium, but cannot be observed in the adult specimens.
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    URL: http://dx.doi.org/10.1007/BF02028388
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    Distribution of succinate-, α-glycerophosphate-, NADH-, and NADPH dehydrogenases (tetrazolium reductases) in the hippocampal region of the rat during postnatal development (1973)
    Springer
    Cell & tissue research 141 (1973), S. 347-373 
    Details
    ISSN: 1432-0878
    Keywords: Hippocampal region (rat) ; Distribution of dehydrogenases ; Postnatal development ; Histochemistry ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Succinate-, α-glycerophosphate-, NADH-, and NADPH dehydrogenases (tetrazolium reductases) were studied histochemically in brain sections. The distribution of these enzymes in the hippocampal region was mapped in different postnatal stages. In addition to the hippocampus and the dentate area which have also been dealt with by other authors, the description includes the entorhinal area, parasubiculum, presubiculum, and subiculum. In all the areas of the hippocampal region the enzyme activity is first localized mainly in cell bodies. Activity in the neuropil shows a tendency to increase with age throughout the stages studied. Examples of particularly early or late differentiation of the histochemical pattern exist. Thus already after 4–6 days the differentiated staining pattern in the hippocampal layers as seen in the adult, begins to appear. In the entorhinal area the early enzyme distribution agrees with that in the adult and facilitates identification of its layers. After 22 days the fundamental enzyme chemoarchitectonics of the adult rat brain has been obtained. Histochemical characteristics of the different areas, support the view that the hippocampal region constitutes an entity.
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    URL: http://dx.doi.org/10.1007/BF00307411
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    Distribution of acetylcholinesterase in the hippocampal region of the rat during postnatal development (1973)
    Springer
    Cell & tissue research 141 (1973), S. 375-400 
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    ISSN: 1432-0878
    Keywords: Hippocampal region (rat) ; AChE distribution ; Postnatal development ; Histochemistry ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Acetylcholinesterase (AChE) in the hippocampal region was studied histochemically in different postnatal stages. Enzyme changes are described for the hippocampus proper, the dentate area, the entorhinal area, parasubiculum, area retrosplenialis e, presubiculum, and subiculum. In 1 day old animals there are only traces of AChE in solitary perikarya of the hippocampal stratum radiatum, while there is no activity in the neuropil. This is in contrast to other regions of the brain, e.g. the corpus striatum and the medial septum. Enzyme activity in the neuropil, although very weak, is seen clearly on the 2nd day. While the enzyme reaction in some cell soma seems to be permanent and also present in adult rats, other cell bodies, e.g. in the entorhinal area lose AChE activity during the last part of the 2nd week and in the 3rd week. Similar observations have been made on other parts of the central nervous system. The main features of chemodifferentiation in the hippocampal region seem to be completed by the end of the third week, although most layers and areas have not yet achieved the same high staining intensity as seen in the adult rats.
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  • 21
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    Effects of parathyroid hormone on osteoclasts in vivo (1973)
    Springer
    Cell & tissue research 141 (1973), S. 529-544 
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    ISSN: 1432-0878
    Keywords: Osteoclast ; Parathyroid hormone ; Lysosomes ; Bone resorption ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Young rats were treated with high doses of parathyroid hormone (PTH). Osteoclasts from these animals revealed characteristic alterations in comparison to control cells: a) The cytoplasm contained large vacuoles with phagocytosed cells, some of which resembled osteoblasts or osteocytes. The vacuoles were interpreted as lysosomes because the engulfed cells often appeared partly digested and the vacuoles contained acid phosphatase as demonstrated histochemically, b) lipid droplets were present in the cytoplasm and usually located close to the endoplasmic reticulum and/or in regions with many free ribosomes, c) the Golgi complex was more frequently separated from the nuclei than in control cells, d) small coated cytoplasmic bodies were numerous in the peripheral cytoplasm, e) the membranes of the endoplasmic reticulum were fused in some places, f) cytoplasmic regions with numerous free ribosomes were frequent, g) large ring-shaped granules occurred in some mitochondria. Energy dispersive X-ray analysis of these granules provided evidence that they contained calcium and probably phosphorus, h) in some osteoclasts the mitochondria were enlarged. — The findings are consistent with an increased activity of osteoclasts and in particular a stimulation of the lysosomal system in these cells.
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    URL: http://dx.doi.org/10.1007/BF00307123
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  • 22
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    Histochemical demonstration of Δ5-3 ß-OHD activity in the granulosa cells of ovarian follicles of immature and mature mice correlated with some ultrastructural observations (1973)
    Springer
    Cell & tissue research 136 (1973), S. 215-228 
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    ISSN: 1432-0878
    Keywords: Ovary (mouse) ; Granulosa cells ; Steroidogenesis ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A systematic study of Δ5-3β-hydroxysteroid dehydrogenase in the granulosa cells of immature and mature mice was made. The histochemical results were compared with the ultrastructural findings on the same cells in an attempt to determine whether the granulosa cells are capable of a steroidogenic role. In newborn and immature mice the granulosa cells of a great amount of follicles demonstrated a moderate or strong histochemical activity. In mature mice the granulosa cells demonstrated a weak or moderate activity normally only in preovulatory follicles and in some other atretic follicles. The granulosa cells of the normal developing follicles did not show such activity. In addition the histological control of numerous parallel sections demonstrated particularly in immature ovaries the presence of a great amount of atretic follicles. In the cytoplasm of the granulosa cells of the follicles in immature ovaries only clusters of lipid droplets with ribosomes were noted; while in the preovulatory follicles of mature animals there started to appear mitochondria with tubular cristae, smooth membranes of the endoplasmic reticulum and irregular lipid droplets. In the obviously atretic follicles several granulosa cells as well as theca interna cells showed numerous lipid droplets and ribosomes together with different degenerating organelles. The granulosa cells of the normal developing follicles showed a well developed Golgi complex, granular endoplasmic reticulum and free ribosomes. The histochemical and ultrastructural findings suggest a steroidogenic role of the granulosa cells only in the larger preovulatory follicles (probably related to an early luteinization of this layer) but this role was not demonstrated in the same cells in normal developing follicles. In addition, since an histochemical positivity was demonstrated also in the granulosa cells of some obviously atretic follicles, it is possible that many of the follicles having granulosa cells filled with lipid droplets and attached ribosomes and histochemically positive might be, in the immature ovaries, in a very precocious stage of atresia. It is to precise for these cells whether a cytoplasm with these two strictly correlated components (lipid droplets and attached ribosomes) and showing an histochemical positivity could carry-on all the biochemical steps involved in steroid biosynthesis or only has only a temporary capability to produce some precursors of steroids.
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    The effects of desiccation and starvation upon the weight, histology and ultrastructure of the reproductive tract of Biomphalaria glabrata, intermediate host of Schistosoma mansoni (1973)
    Springer
    Cell & tissue research 136 (1973), S. 229-262 
    Details
    ISSN: 1432-0878
    Keywords: Reproductive tract ; Biomphalaria glabrata ; Desiccation ; Starvation metabolism ; Weighing experiments ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of desiccation (36 weeks), starvation (12 weeks) and recovery (4 weeks following 16 weeks of desiccation and 2 weeks following 9 weeks of starvation) upon the accessory sex glands and some other body parts of Biomphalaria glabrata were studied by means of weighing experiments and histochemical and ultrastructural methods. The results of the weighing experiments show that the various parts of the reproductive tract are not protected from involution processes during the adverse conditions, as some parts (the albumen gland, spermduct and prostate gland) showed a more pronounced decrease in weight than other body parts. Measurements of the epithelial heights of the accessory sex glands and ultrastructural observations corroborate the results of the weighing experiments. During the adverse conditions the organelles involved in the formation of the secretion granules (granular endoplasmic reticulum and Golgi-apparatus) became involuted. The secretion granules within the cells of the albumen gland, spermduct and prostate gland were broken down by crinophagy. This latter process was less pronounced in the oviduct, muciparous gland, oöthecal gland, uterus and vagina. During the adverse conditions the lipoprotein producing cell types (mainly in the male part) remained active longer than the polysaccharide producing cell types (mainly in the female part). The significance of this observation is discussed. The amounts of glycogen present in most tissues of the reproductive tract and of the other investigated body parts (the foot, columellar muscle and vesicular connective tissue cells) were not apparently diminished, even when the secretory cells showed clear signs of involution, as a result of long periods of adverse conditions. Within the recovery periods 50% of the snails resumed egg production. During recovery the relative increases in weight of the accessory sex glands seemed to exceed that of other body parts, indicating that the restoration of the accessory sex glands is favoured. Histologically, reactivation of the accessory sex glands was apparent after 36–72 hours of recovery. At this time secretory material was again observed within the cisternae of the gland cell Golgi-bodies. Because reactivation is fairly rapid, a nervous or hormonal control of this process appears more likely than control via the general metabolism. The possible functions of various body organs in storing food reserve material is discussed, and it is concluded that the glycogen storing vesicular connective tissue cells (which occur throughout the whole body in the connective tissue) play a major role.
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    Development and differentiation of fetal rat sensory ganglia and spinal cord segments in vitro (1973)
    Springer
    Cell & tissue research 144 (1973), S. 339-351 
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    ISSN: 1432-0878
    Keywords: Nervous system ; Tissue culture ; Sheath cells ; Sensory end-organs ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fetal spinal ganglia and spinal cord segments with adhering spinal ganglia were explanted on collagen-coated coverslips. They were investigated with enzyme histochemical methods for the existence of hydrolases and dehydrogenases up to 54 days of cultivation. Alkaline phosphatase was located in arachnoid cells and in mantle cells (satellite cells). Butyrylcholinesterase and alpha-glycerophosphate-menadione-tetrazolium reductase were found in mantle cells. Acetylcholinesterase and indoxylesterase were active in the whole neuron; acid phosphatase and alpha-naphthylacetate esterase were restricted to the perikarya. During the process of cell differentiation in vitro alkaline phosphatase decreased in mantle cells. Acid phosphatase became diminished distinctly in spinal ganglion cells. The other neuronal enzymatic activities remained unaltered during the whole period of cultivation. Proliferated Schwann cells were conspicuous by their activity for butyrylcholinesterase. In newly formed myelin sheaths arylsulphatase was active. Lactate dehydrogenase was contained in the perineurium which had developed. Cultures of long duration could contain cytological formations which were reminiscent of sensory end-organs with respect to their enzyme patterns. The enzyme activities of nervous tissues in vitro in their approximation to the situation in situ are discussed.
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    Topochemischer Nachweis von carbonylhaltigen Verbindungen im Thymus (1973)
    Springer
    Cell & tissue research 142 (1973), S. 465-477 
    Details
    ISSN: 1432-0878
    Keywords: Rabbit thymus ; Ketosteroids ; Granulated cells ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Topochemisch konnten im Kaninchenthymus Ketosteroide nachgewiesen werden, für deren Vorhandensein die chemische Analyse von Thymuslipidextrakten Hinweise ergeben hatte. Die Darstellung der Ketosteroide erfolgte mit der NAHD-Reaktion (Camber, 1949). Diese Befunde dürften als spezifisch gelten, da eine Verfälschung durch freie Gewebsaldehyde, Plasmalogene (Gomori, 1952) und Corticosteroide (Khanolkar et al., 1958) ausgeschlossen wurde. Mit Hilfe der Camber-Methode konnten Granula bestimmter Zellen des Kaninchenthymus selektiv dargestellt werden, die auf Grund morphologischer Kriterien in zwei Gruppen unterteilt wurden. Die Zellen liegen im gesamten Thymusparenchym sowie im Bindegewebe der Septen und der sogenannten „Kapsel“. Prädilektionsorte stellen die Umgebung der Hassallschen Körperchen, die Mark-Rinden-Grenze, der intra- und perivasale Raum sowie die subkapsuläre Zone dar. Beide Zellgruppen sind Sudan III-, PAS- und Eosin-positiv, besitzen eine ausgeprägte gelbgrüne Eigenfluoreszenz und können differentialdiagnostisch gegen Mastzellen abgegrenzt werden. Elektronenmikroskopisch zeigen beide Zellgruppen zahlreiche Übereinstimmungen mit Reifestadien eosinophiler Knochenmarkszellen.
    Notes: Summary Ketosteroids were demonstrated topochemically in the rabbit thymus following indications given by chemical analysis of thymus lipid extracts. They were revealed by means of the NAHD-reaction (Camber, 1949). The results are thought to be specific, because adulteration by free tissue aldehydes, plasmalogens (Gomori, 1952) and corticosteroids (Khanolkar et al., 1958) can be excluded. The Camber-method selectively reveals the presence of two groups of morphologically differentiable granules in specific rabbit thymus cells which are distributed throughout the entire thymic parenchyme as well as in the connective tissue of the septa and the in so-called “capsule”. They are mainly seen in the vicinity of Hassall's bodies, in the zone between medulla and cortex, the intra- and perivascular space and in the subcapsular space. Both cell groups are Sudan III-, PAS- and eosin-positive, show intensive yellow-green primary fluorescence and can be distinguished from mast cells by differential diagnosis. Electron microscopy reveals that both cell groups show many similarities with the maturation phase of bone marrow eosinophils.
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    URL: http://dx.doi.org/10.1007/BF00306710
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    Fine structure and innervation of the avian adrenal gland (1973)
    Springer
    Cell & tissue research 145 (1973), S. 389-416 
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    ISSN: 1432-0878
    Keywords: Adrenal chromaffin cells ; Ganglion cells ; Avian adrenal gland ; Light- and electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the adrenal gland of 15 bird species was investigated by light-, fluorescence-, and electron microscopy as well as by histochemical methods. 1. Adrenal Chromaffin Cells. Cells producing adrenaline (A) and noradrenaline (NA) may be distinguished from each other with the electron microscope by the different structure of their granules. In all species investigated A-cells prevailed. Granules in A-cells of Corvidae, partly those of the other passeriform birds as well, resemble granules from the pituitary pars intermedia because of their incomplete membrane and the low electron density of their contents. A-cells of some passeriform birds have mitochondria with specifically arranged cristae. Large cytosomes and inclusions of lipofuscin pigment are to be found in the gulls Larus ridibundus and Larus argentatus. The House Sparrow (Passer domesticus) and the Chaffinch (Fringilla coelebs) show vacuoles filled with a colloid-like substance, which are surrounded by adrenal chromaffin cells. 2. Ganglion Cells. The adrenal gland of birds is rich in ganglion cells. Two types can be distinguished. “Large” ganglion cells resemble completely sympathetic nerve cells as described in many vertebrates. “Small” ganglion cells have a low nucleus-cytoplasm-ratio. Some of them include granules of various structure and quantity. Whether they represent transitional stages to adrenal cells, interneurons, P-neurons or a heterogeneous population remains to be clarified.
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    Fine structure and innervation of the avian adrenal gland (1973)
    Springer
    Cell & tissue research 145 (1973), S. 417-442 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal chromaffin cells ; Birds ; Cholinergic innervation ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary According to their ultrastructure and histochemistry three types of efferent nerve fibers can be distinguished in the bird's adrenal gland. The main part is made up of cholinergic fibers recognizable by a positive reaction for acetylcholinesterase and two specific populations of granules within the synaptic ending. Synaptic vesicles measuring 300 to 500 Å in diameter and dense-cored vesicles with a diameter of about 1 000 Å are discernible. In the periphery of the gland cholinergic axons for the innervation of adrenal cells form large bundles surrounded by a perineural sheath. The bundles cross the capsule and are situated within the adrenal chromaffin cords or at their periphery. Finally small groups of fibers enter a group of chromaffin cells which are surrounded by a basal lamina and which consist of about a dozen or more cells producing adrenaline and noradrenaline. Synaptic endings occur, above all in passeriform species, in the center of a chromaffin cell complex. They are either attached to the innervated cells or their dendrite-like processes, or embedded into the cells, or connected to short spines of the innervated cells. Synaptic and dense-cored vesicles leave the bouton by exocytosis. One synaptic terminal may innervate up to three A- or NA-cells. The existence of different types of synapses for A- and NA-cells cannot be excluded.
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    Elektronenmikroskopische und histochemische Untersuchungen zur Funktion der Branchialdrüse (Parabranchialdrüse) der Cephalopoda (1973)
    Springer
    Cell & tissue research 139 (1973), S. 253-269 
    Details
    ISSN: 1432-0878
    Keywords: Branchial gland ; Cephalopoda ; Fine structure ; Hemocyanin ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Licht- und elektronenmikroskopische Untersuchungen an der Branchialdrüse verschiedener Cephalopoden (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) zeigen, daß das Drüsengewebe des stark vaskularisierten Organs aus einem sekretorisch aktiven Zelltypus mit ausgeprägtem endoplasmatischen Retikulum besteht. In den Zisternen des endoplasmatischen Retikulums lassen sich granuläre und ringförmige Strukturen (Durchmesser: 45–65 Å und 170 Å) nachweisen, die in Größe und Gestalt mit den Hämocyanin-Einheiten in den Gefäßen und Lakunen übereinstimmen und durch randlichen Zerfall der Zellen in den Blutraum auszuwandern scheinen. Die histochemischen Untersuchungen ergeben eine positive Reaktion für Monoaminoxydase, aber vergleichsweise geringe Phosphatase- und Dehydrogenase-Aktivitäten. Diese Befunde sowie der histochemisch nachgewiesene hohe Kupfergehalt des Drüsengewebes sprechen für eine hämopoetische, d.h. hämocyaninbildende Funktion der Kiemendrüse.
    Notes: Summary Light and electron microscopic investigations on the branchial gland of different Cephalopoda (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) show that the gland tissue, strongly vascularized, consists of an actively secreting cell type with a remarkable endoplasmatic reticulum. In the cisternae of the endoplasmatic reticulum granular and ring-shaped structures can be identified (diameter: 45–65 Å and 170 Å) which correspond in size and shape with the hemocyanin units in the vessels and lacunae and which seem to emigrate into the blood space caused by the peripheral decay of the cells. Histochemical investigations show a positive reaction for monoaminoxidase, but comparatively low phosphatase and dehydrogenase activities. These results, and also the high content of histochemically identified copper in the gland tissue, seem to indicate a hemopoetic, i.e. hemocyanin-forming function for the branchial gland.
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    The distribution of monoamines in the tel-, di-and mesencephalon of Xenopus laevis tadpoles, with special reference to the hypothalamo-hypophysial system (1973)
    Springer
    Cell & tissue research 137 (1973), S. 521-540 
    Details
    ISSN: 1432-0878
    Keywords: Brain ; Hypothalamo-hypophysial system ; Xenopus laevis, tadpoles ; Monoamines ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In tadpoles of Xenopus laevis the histochemical distribution of monoamines in the tel-, di- and mesencephalon was studied. Catecholamines are present in some mitral cells of the olfactory bulb, the neurons of the preoptic recess organ (PRO), the neurons of the paraventricular organ (PVO) and cells in the vicinity of the PVO, namely the so-called PVO-accompanying cells, the nucleus infundibularis dorsalis (NID) and in the nucleus reticularis mesencephali (NRM). The PRO, PVO, and NID are composed of liquor-contacting neurons. With the Falck-technique, in the PVO and NID two types of fluorescent cells were observed: green cells, containing a catecholamine and yellow-orange cells, presumably containing a tryptamine. Tryptaminergic neurons occur in the NRM and in the area of the raphe nucleus. Extensive tracts of fluorescent fibres originating in the hindbrain ascend to the area praeoptica and the septum, striatum and epistriatum of the telencephalon. Also, fibre connections could be traced from the PVO to the area praeoptica, to the thalamus and to the nucleus ventromedialis tuberis. The PVO and/or NID give rise to a catecholaminergic tract running towards the hypophysis. Fibres of this tract partly terminate in the developing median eminence and partly in the pars intermedia. Experimental data indicate that the aminergic fibres in the pars intermedia exert an inhibitory influence on MSH-release.
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    URL: http://dx.doi.org/10.1007/BF00307228
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    Cholinesterase-containing nerve fibres on blood vessels in lungs of some laboratory mammals (1973)
    Springer
    Cell & tissue research 140 (1973), S. 91-100 
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    ISSN: 1432-0878
    Keywords: Pulmonary and bronchial vessels ; Laboratory mammals ; Cholinesterase-containing nerve fibres ; Species differences ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Distribution of cholinesterase-containing nerve fibres on blood vessels in the lungs of rats, mice, rabbits, guinea pigs, cats, dogs, and rhesus monkeys has been studied with the thiocholine method modified by Coupland and Holmes and with the “direct-coloring” method according to Karnovsky and Roots. The presence of acetylcholinesterase positive nerve fibres on the pulmonary arteries has been established only in four species of the studied animals, namely the cat, the dog, the rhesus monkey and the rabbit. These nerves form d distinct plexus on the border between the media and adventitia. In the thick walled perihilar branches of the pulmonary artery of the rabbit the nerve fibres penetrate—as a rule—into the outer half of the media. Despite many times repeated experiments and careful investigations no nerve fibres have been found on the intrapulmonary branches of the blood vessels in the lungs of rats, mice, and guinea pigs. In the walls of the pulmonary veins, with the exception of the pulmonary veins in the rat and the mouse, cholinesterase fibres have been identified only seldom. Acetylcholinesterase nerve fibres in medio-adventitial localization have also been found in the walls of bronchial arterioles, this being the case in all the species under study. Bronchial veins do not exhibit any nerve fibres. The distribution of the acetylcholinesterase-containing nerve fibres on the pulmonary arteries is different from species to species.
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    Aspects histologique, histochimique et ultrastructural des péricaryones neurosécréteurs latéraux du protocérébron de Locusta migratoria migratorioides (Insecte: Orthoptère) (1973)
    Springer
    Cell & tissue research 141 (1973), S. 75-91 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretory cells ; Lateral ; Locust ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'étude histologique, histochimique et ultrastructurale des péricaryones neurosécréteurs latéraux de Locusta précise leur nombre et leur localisation. Elle révèle que le neurosécrétat latéral est semblable morphologiquement (forme et diamètre des granules élémentaires) au neurosécrétat A de la pars intercerebralis. Cependant, il en diffère histochimiquement par la présence d'une composante glucidique. Elle confirme l'hypothèse émise pour les péricaryones neurosécréteurs de la pars intercerebralis (Girardie et Girardie, 1967), de l'unicité cellulaire chez Locusta des types A et B selon la nomenclature de Johansson (1958). Elle suggère que les péricaryones latéraux pourraient être des cellules neurosécrétrices très actives.
    Notes: Summary The number (8 to 12) and position of the lateral neurosecretory cells have been established in Locusta by a histological, histochemical and ultrastructural study. The neurosecretory material of the lateral cells contains glycoprotein and is, in this way, histochemically different from medial A cells neurosecretory material. However, the morphological aspect (shape, diameter) of elementary dense core vesicles in the lateral and medial A neurosecretory cells is similar. This study confirms the idea (Girardie and Girardie, 1967) that, in Locusta, A cells and B cells (Johansson, 1958) are in fact two physiological aspects of one cell type. It also suggests that the lateral cells could be very active neurosecretory cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307397
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    Parasympathetic and sympathetic postganglionic synapses in ureterovesical autonomic pathways (1973)
    Springer
    Cell & tissue research 146 (1973), S. 147-154 
    Details
    ISSN: 1432-0878
    Keywords: Peripheral autonomic innervation ; Ureterovesical ganglia ; Parasympathetic postganglionic synapse ; Sympathetic postganglionic synapse ; Denervated bladder ; Acetylcholinesterase ; Catecholamine ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histochemical techniques for acetylcholinesterase and catecholamine show that ureterovesical ganglia of both cat and dog contain dense intraganglionic cholinergic and adrenergic plexuses. Ramifications of both plexuses surround most cholinergic and adrenergic ganglion cell bodies as pericellular synaptic plexuses. Similar pericellular plexuses exist around extraganglionic cholinergic and adrenergic ganglion cells. Both adrenergic and cholinergic synaptic fibers persist in denervated pregnaglionic nerve-free specimens, indicating that cholinergic synaptic fibers are postganglionic parasympathetic in nature. The presence of adrenergic (postganglionic sympathetic) and postganglionic parasympathetic synapses around cell bodies in ureterovesical ganglia provides a morphologic basis for the sympathoinhibitory and muscarinic parasympatho-excitatory phenomena described in these ganglia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307343
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    Reinigung Pesticidrüskstände enthaltender Rohextrakte mit einer automatisch arbeitenden Apparatur nach dem Prinzip der kombinierten Spül- und Codestillation (Sweep Co-Distillation) (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 263 (1973), S. 120-127 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Pesticiden ; Chromatographie, Gas ; Apparatur zur Reinigung von Rohextrakten durch Spül- und Codestillation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Eine automatisch arbeitende Apparatur zur Reinigung pflanzlicher Rohextrakte auf der Basis der kombinierten Spül- und Codestillation für die gas-chromatographische Rückstandsbestimmung von Insecticiden wurde entwickelt. Mit dieser Apparatur wurden als Beispiel Rohextrakte von 27 phosphorhaltigen Pesticiden in 13 Erntegütern nach einheitlichen Bedingungen mit durchschnittlichen Ausbeuten von 66,4 bis 112,4% gereinigt. Dabei lagen die Rückstände im Konzentrationsbereich der durch die deutsche Höchstmengenverordnung festgelegten Toleranzen. Die Anwendungsmöglichkeiten der Apparatur sind erweiterungsfähig.
    Notes: Abstract An automatic apparatus has been constructed which cleans up crude extracts basing upon the principle of “sweep co-distillation” for gas-chromatographic residue analysis of insecticides. With this instrumentation, e.g. crude extracts from 13 crops containing 27 phosphorus pesticides has been cleaned up using unique conditions. Average recovery values reach from 66.4 to 112.4%. Concentration levels of the residues had been elected in accordance with the German tolerance list. The apparatus is suitable for further applications.
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    URL: http://dx.doi.org/10.1007/BF00424355
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    Gas-chromatographic determination of methylester end groups in polyethylene terephthalate (PET) and ester-interchange products (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 293-296 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Methylesterendgruppen in Polyäthylenterephthalat ; Chromatographie, Gas ; Zersetzung mit Hydrazin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A method for the determination of methylester end groups in polyester and ester-interchange product is described. The sample is decomposed by boiling with hydrazine hydrate. The methanol liberated is determined by gas chromatography using Tenax GC as column packing and ethanol as internal standard. The gas chromatographic determination has passed through a number of development stages, viz. backflush and run methods.
    Notes: Zusammenfassung Eine Methode für die Bestimmung von Methylesterendgruppen in Polyäthylenterephthalat und Umesterungsprodukten wird beschrieben. Die Probe wird mit Hydrazinhydrat unter Rückfluß zersetzt. Das freigesetzte Methanol wird gas-chromatographisch bestimmt. Als Trennphase wird Tenax GC und als innerer Standard Äthanol verwendet. Die gas-chromatographische Bestimmung wurde über die „run method“ und „backflush method“ entwickelt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00426418
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    Gas-Chromatographie von Tl, Se, Te, Hg, As, Sb, Bi, Sn als Phenylverbindungen (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 301-303 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Thallium, Selen, Tellur, Quecksilber, Arsen, Antimon, Wismut, Zinn ; Chromatographie, Gas ; Abtrenn. als Phenylverbindung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Phenylverbindungen von Thallium, Selen, Tellur, Quecksilber, Arsen, Antimon, Wismut und Zinn sind zur gas-chromatographischen Analyse dieser Elemente geeignet. Sie entstehen bis auf Thalliumtriphenyl quantitativ beim Umsatz der aus saurer Lösung extrahierten Diäthyldithiocarbamidate mit magnesiumorganischen Verbindungen. Gas-chromatographisch lassen sich die Phenylverbindungen aller acht Elemente mit einer Säule unter Verwendung eines Temperaturprogramms trennen. Im Falle des Quecksilberdiphenyls konnte keine vollständige Elution erreicht werden.
    Notes: Abstract The phenyl compounds of thallium, selenium, tellurium, mercury, arsenic, antimony, bismuth and tin are suitable for the analysis of these elements by gas chromatography. They are produced quantitatively except thallium triphenyl when the diethyldithiocarbamates, which are extracted out of acid solution, are reacted with magnesium organic compounds. By gas chromatography the phenyl compounds of all eight elements are separated with one column by utilizing a temperature program. In case of mercury diphenyl complete elution could not be realized.
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    URL: http://dx.doi.org/10.1007/BF00426420
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    Glimmer als Trennfüllung in der Gas-Chromatographie (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 399-400 
    Details
    ISSN: 1618-2650
    Keywords: Chromatographie, Gas ; Glimmer als Trennfüllung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Trotz der vorwiegend negativen Eigenschaften, die Glimmer als Trennsäulenfüllung zeigt, erlaubt die überlegene Selektivität als Molekularsieb speziell die Trennung flach gebauter Moleküle.
    Notes: Abstract Though mica has mainly negative properties in separation columns its supreme selectivity as a molecular sieve enables separations of molecules with flat shape.
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    URL: http://dx.doi.org/10.1007/BF00423643
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    Analysis of polyethylene oxide adducts by cleavage with hydrobromic acid in acetic acid and subsequent gas-chromatographic determination of the reaction products (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 401-406 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von Polyäthylenoxidaddukten ; Chromatographie, Gas ; Spaltung mit HBr
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A method is discussed by which both the ethylene oxide content and the nature and distribution of the alkyl chains of polyethylene oxide adducts can be determined in one step. Until recently the quantitative analysis of these products gave rise to many difficulties. The method includes a fission of the polyethylene oxide adducts with a 40% solution of hydrobromic acid in glacial acetic acid in sealed glass tubes, after which the fission products are determined gas-chromatographically. Results are given for saturated as well as unsaturated ethoxylated fatty alcohols, phosphated polyoxyethylene alkyl ethers, ethoxylated amines and ethoxylated esters.
    Notes: Zusammenfassung Eine Methode wird beschrieben, die es ermöglicht, sowohl den Äthylenoxidgehalt wie die Art und Verteilung der Alkylketten von Polyäthylenoxidaddukten in einem Gang festzustellen. Bisher waren mit der quantitativen Analyse dieser Produkte viele Schwierigkeiten verbunden. Die Polyäthylenoxidaddukte werden in abgeschmolzenen Glasröhren mit einer 40% igen Lösung von Bromwasserstoffsäure in Eisessig gespalten, wonach die Spaltungsprodukte gas-chromatographisch identifiziert werden. Einige mit dieser Methode an gesättigten sowie ungesättigten äthoxylierten Fettalkoholen, phosphatierten Polyoxyäthylenalkyläthern, äthoxylierten Aminen und äthoxylierten Estern erzielte Resultate werden erörtert.
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    URL: http://dx.doi.org/10.1007/BF00423644
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    Gas-chromatographische Bestimmung einiger pharmazeutisch wichtiger Sulfonamide (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 266 (1973), S. 358-360 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Sulfonamiden ; Chromatographie, Gas ; Methylderivate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Eine neue Methode zur Trennung und Identifizierung von 15 Sulfonamiden wird beschrieben. Die Sulfonamide werden mit Diazomethan in die N1-Methyl-Derivate umgewandelt. Gas-chromatographisch erfolgt dann die Auftrennung des Gemisches der Derivate. Die relativen Retentionszeiten von 15 Sulfonamiden bzw. deren Methylderivaten werden mitgeteilt. Der Zeitaufwand für die Analyse beträgt eine Stunde.
    Notes: Abstract With diazomethane the sulfonamides are converted into their N1-methyl-derivatives. The mixture of these derivatives is analysed by gas-chromatography. The relative retention-times of 15 sulfonamides respectively of their methyl-derivatives are given. The whole analysis can be achieved within 1 h.
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    URL: http://dx.doi.org/10.1007/BF00423765
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    Oestron-dimethylphosphinsäureester, ein Beispiel einer neuen Derivatklasse zum empfindlichen, gas-chromatographischen Nachweis von Steroiden (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 28-30 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Steroiden, Oestron als Dimethylphosphinsäureester ; Chromatographie, Gas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Darstellung eines neuartigen, zum gas-chromatographischen Nachweis geeigneten Phosphorderivats von Oestron wird beschrieben. Der Oestron-dimethylphosphinsäureester läßt sich sehr empfindlich mit dem Alkaliflammenionisationsdetektor (AFID) nachweisen. Die Umsetzung von Dimethylaminodimethylphosphin mit Oestron ist unter den angegebenen Bedingungen quantitativ und sehr gut reproduzierbar. Bezugskurven mit Oestronkonzentrationen von 2–50 μg Oestron im Ansatz hatten bei Nachweis mit dem FID einen Korrelationskoeffizienten von 0,999, bei Nachweis mit dem AFID von 0,998. 200 pg des Derivats konnten noch gut gemessen werden. Die Reaktionsbedingungen werden diskutiert. Auf die erfolgreiche Derivatisierung anderer Steroidhormone wird abschließend hingewiesen.
    Notes: Abstract The synthesis of estrone-dimethyl phosphinic ester, with good gas chromatographic properties is described. This ester is very sensitively detectable with the alkali detector. The reaction of dimethyl-amino-dimethylphosphine with estrone is quantitative and reproducible. Calibration curves with concentrations of estrone varying from 2 to 50 μg per sample detected by a FID yielded a correlation coefficient of 0.999, detected by an AFID one of 0.998. The smallest amount which could be measured was 200 pg. The reaction conditions are discussed. The possibility of the derivatisation of other steroid hormones is shown.
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    URL: http://dx.doi.org/10.1007/BF00635413
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    Untersuchungen zur mikroanalytischen Bestimmung der langkettigen Fettsäuren im Blut (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 342-346 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Fettsäuren in Blut, Plasma ; Chromatographie, Gas ; Mikroanalyse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Für die im Plasma an Albumin gebundenen sog. „freien Fettsäuren“ (FFS) wird ein neues gas-chromatographisches Analysenverfahren vorgestellt. Das Probenvolumen beträgt 50 μl Serum oder Plasma. Die Extraktion erfolgt in 1,1 ml CHCl3/CH3OH (2∶1, v/v) mit n-Heptadecansäure als innerem Standard (0,05 μVal C17∶0/ml). Nach Eindampfen am Rotationsverdampfer werden die FFS im verschlossenen Reaktionskölbchen mit 20 μl CH3J/Essigsäureäthylester (1∶1, v/v) über festem K2CO3 bei 90°C 1 h verestert. Die Varianz des Verfahrens errechnet sich bei 15 Parallelbestimmungen zu 4,85%.
    Notes: Abstract A new microanalytical assay on the basis of gas chromatography is demonstrated. Volume of samples: 50 μl of serum or plasma. Extraction with 1.1 ml CHCl3/CH3OH (2∶1, v/v) solution of n-heptadecanoic acid (0.05 mVal/l) as an internal standard. After evaporation with a rotating evaporator the acids are esterified in closed vessels with 20 μl of CH3J/ethyl acetate (1∶1, v/v) over solid K2CO3 at 90° C for 1 h. The variance of the procedure was 4.85% in 15 parallel determinations.
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    URL: http://dx.doi.org/10.1007/BF00424144
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    Gas-chromatographischer Nachweis von Lösungsmittelspuren und Metaboliten im Blut (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 347-350 
    Details
    ISSN: 1618-2650
    Keywords: Nachw. von Lösungsmitteln in Blut ; Chromatographie, Gas ; Spurenanalyse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es werden die Nachweismöglichkeiten von Lösungsmittelspuren und deren Metaboliten im Blut diskutiert. Es wird hierbei den modernen gas-chromatographischen Methoden einschließlich Dampfraum-Gas-Chromatographie mit Elektroneneinfangdetektor der Vorrang vor allen anderen Methoden eingeräumt und anhand eines Beispieles der Überwachung von Trichloräthylenarbeitern die Konzentration der Metaboliten Trichloräthanol und Trichloressigsäure im Blut beschrieben.
    Notes: Abstract The possibilities of detecting traces of solvents and their metabolites in blood are discussed, whereby the modern gas chromatographic methods including head-space gas chromatography with ECD is given precedence of all other methods. By means of an example of the surveillance of men working with trichloroethylene the concentration of the metabolites trichloroethanol and trichloroacetic acid in blood is described.
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    URL: http://dx.doi.org/10.1007/BF00424145
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