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  • Articles  (28)
  • Light microscopy  (28)
  • Springer  (28)
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  • 2020-2020
  • 1970-1974  (28)
  • 1945-1949
  • 1974  (11)
  • 1973  (17)
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  • Articles  (28)
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  • Springer  (28)
  • Wiley
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  • 2020-2020
  • 1970-1974  (28)
  • 1945-1949
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 12 (1973), S. 125-136 
    ISSN: 1432-0827
    Keywords: Mineralization ; Molt ; Isopod ; Chemistry ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Au cours de la phase inhabituelle de mue d'un isopode d'eau courante,Lirceuts brachyrus, la moitié postérieure de l'exosquelette est éliminée 24 heures avant la moitié antérieure. A ce stade, une reminéralisation se développe dans la partie postérieure alors que la partie antérieure est dans un stade de pré-mue. Le pourcentage de différence en calcium dans les deux moitiés à mi-mue et mue complète est respectivement de 22% (p〈0.01) et 33% (p〈0.01), indiquant une complexation du calcium pendant la mue. La rapidité de la reminéralisation est illustrée par le fait que le contenu minéral total double dans la partie postérieure entre la mi-mue et la mue totale et dans la partie antérieure entre la fin de la mue et un jour après. Le carbonate de calcium, sous forme de calcite, a pu être identifié par diffraction électronique de coupes fines des téguments.
    Abstract: Zusammenfassung Während der ungewöhnlichen Häutungssequenz des Frischwasser-IsopodenLirceus brachyurus (Harger) wird die hintere Hälfte des äußeren Skeletts 24 Std vor der vorderen Hälfte abgestoßen. In der halbgehäuteten Phase erfolgt Remineralisation im hinteren Teil, während der vordere Teil in einem Vorhäutungszustand ist. Der prozentuale Unterschied des Calciums in den zwei Hälften bei Halb- und Vollhäutungszustand ist 22% (p〈0,01) bzw. 33% (p〈0,01), was andeutet, daß Calcium während der Häutung abgesondert wird. Die Geschwindigkeit der Remineralisation erhellt aus der Tatsache, daß sich der Gesamtmineralgehalt im hinteren Teil zwischen Halt- und Vollhäutung, in der vorderen Hälfte jedoch zwischen Endhäutung und einem Tag nach der Häutung verdoppelt. Calciumcarbonat in kristalliner Calcitform wurde mittels Elektronendiffraktion von dünnen Hautschnitten nachgewiesen.
    Notes: Abstract During the unusual molt sequence of the fresh-water isopod,Lirceus brachyrus (Harger), the posterior half of the exoskeleton is shed 24 hours before the anterior half. At the half-molt stage, occurs in the posterior part while the anterior portion is in a pre-molt condition. The percentage difference in calcium in the two halves at half-molt and full-molt is22 (p〈0.01) and33 (p〈0.01) respectively, an indication that calcium is sequestered during The rapidity of remineralization is illustrated by the fact that the total mineral content doubles in the posterior part between half and full molt and in the anterior half between the end of molt and one day after ecdysis. Calcium carbonate in the calcite cystalline form was demonstrated by electron diffraction of thin sections of the integument.
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  • 2
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    Springer
    Cell & tissue research 139 (1973), S. 179-200 
    ISSN: 1432-0878
    Keywords: Retino-preoptic pathway ; Optic tracts ; Rana temporaria ; Light microscopy ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The optic tracts and centres of optic terminals of Rana temporaria have been investigated with silver impregnation techniques after unilaterally cutting the optic nerve and autoradiographically after injection of a mixture of tritiated amino acids into the vitreous body of the left eye. The observations on the course of the optic tracts and on the optic terminals in the thalamus and optic tectum are to a great extent in agreement with those of other authors. The probability of a retino-preoptic pathway is supported by the detection of fibres running in dorsal direction in front of the place where the optic nerve penetrates the brain. In horizontal sections these fibres can be seen deviating from the optic tract, covering a short distance in frontal direction and then turning upward under an angle of about 90 degrees. They disappear between the ventral aldehyde-fuchsin positive cells of the preoptic nucleus. The ventral and median parts of the ipsilateral preoptic nucleus contain fragments of degenerated fibres. Autoradiographic data are also in favour of the presence of an ipsilateral retino-preoptic tract. After carefully counting the number of grains over left and right preoptic nucleus, it appeared that over the ventral and median parts of the left preoptic nucleus more grains occur than over the identical areas of the right one.
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  • 3
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    Cell & tissue research 139 (1973), S. 473-485 
    ISSN: 1432-0878
    Keywords: Pineal gland ; Rabbit ; Architecture ; Autonomic innervation ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A light microscopic investigation of the rabbit pineal gland with the aid of silver-stained sections gave the following results. In the gland a medulla and a cortex can be distinguished, the medulla containing so-called light and dark pinealocytes, the cortex only light ones. Autonomic nerve fibres reach the pineal organ by two routes: (1) via the perivascular spaces of pineal blood vessels and (2) via two distinct nerve bundles, the nervi conarii. Bilateral superior cervical ganglionectomy revealed that these pinealo-petal nerve fibres are mainly orthosympathetic postganglionic. Intramural pineal neurones with synaptic-like structures on their cell bodies and dendrites point to the presence of a parasympathetic innervation next to the orthosympathetic one. Direct afferent or efferent neural connections with the brain appeared to be absent.
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  • 4
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Rat ; Gonadotrophic cells ; Cycle, influence of testosterone ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 4-day cyclic adult female Wistar rats were injected subcutaneously with testosterone propionate on diestrus 1 at 16:00 and on diestrus 2 at 10:00 respectively. Non-injected females served as controls. Autopsy was performed on diestrus 2 at 23:00, and on proestrus at 14:00 and 17:00 respectively. The blue Alcian-PAS staining was used to evidence FSH (β) and LH (γ) pituitary cells. In control animals and in diestrus 2 injected females only a small number of FSH cells could be detected on diestrus 2 at 23:00. This number increased markedly on proestrus at 14:00 and decreased on proestrus at 17:00. A similar evolution was observed in diestrus 1 testosterone injected females, but the number of FSH cells appeared higher at any stage of autopsy in these females than in diestrus 2 injected females and in control rats. In control females, numerous LH cells were observed on diestrus 2 at 23:00. The number of these cells was diminished on proestrus at 14:00 and still more at 17:00. On the contrary few LH cells were detected in testosterone injected females on the evening of diestrus 2. An increase of these cells occurred on proestrus at 14:00, followed at 17:00 by only a weak diminution as established by comparison with control animals. An inhibition of FSH release and a suppression of the proestrus surge of LH were therefore supposed to cause, on one hand, the slowing up of follicular growth observed in diestrus 1 injected females and, on the other hand, the blockage of ovulation noted in both diestrus 1 and diestrus 2 treated animals.
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  • 5
    ISSN: 1432-0878
    Keywords: Hippocampal region (rat) ; Distribution of dehydrogenases ; Postnatal development ; Histochemistry ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Succinate-, α-glycerophosphate-, NADH-, and NADPH dehydrogenases (tetrazolium reductases) were studied histochemically in brain sections. The distribution of these enzymes in the hippocampal region was mapped in different postnatal stages. In addition to the hippocampus and the dentate area which have also been dealt with by other authors, the description includes the entorhinal area, parasubiculum, presubiculum, and subiculum. In all the areas of the hippocampal region the enzyme activity is first localized mainly in cell bodies. Activity in the neuropil shows a tendency to increase with age throughout the stages studied. Examples of particularly early or late differentiation of the histochemical pattern exist. Thus already after 4–6 days the differentiated staining pattern in the hippocampal layers as seen in the adult, begins to appear. In the entorhinal area the early enzyme distribution agrees with that in the adult and facilitates identification of its layers. After 22 days the fundamental enzyme chemoarchitectonics of the adult rat brain has been obtained. Histochemical characteristics of the different areas, support the view that the hippocampal region constitutes an entity.
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  • 6
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    Cell & tissue research 141 (1973), S. 375-400 
    ISSN: 1432-0878
    Keywords: Hippocampal region (rat) ; AChE distribution ; Postnatal development ; Histochemistry ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Acetylcholinesterase (AChE) in the hippocampal region was studied histochemically in different postnatal stages. Enzyme changes are described for the hippocampus proper, the dentate area, the entorhinal area, parasubiculum, area retrosplenialis e, presubiculum, and subiculum. In 1 day old animals there are only traces of AChE in solitary perikarya of the hippocampal stratum radiatum, while there is no activity in the neuropil. This is in contrast to other regions of the brain, e.g. the corpus striatum and the medial septum. Enzyme activity in the neuropil, although very weak, is seen clearly on the 2nd day. While the enzyme reaction in some cell soma seems to be permanent and also present in adult rats, other cell bodies, e.g. in the entorhinal area lose AChE activity during the last part of the 2nd week and in the 3rd week. Similar observations have been made on other parts of the central nervous system. The main features of chemodifferentiation in the hippocampal region seem to be completed by the end of the third week, although most layers and areas have not yet achieved the same high staining intensity as seen in the adult rats.
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  • 7
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    Cell & tissue research 142 (1973), S. 131-146 
    ISSN: 1432-0878
    Keywords: Epidermis (mouse) ; Pattern of organization ; Proliferation kinetics ; Three-dimensional analysis ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Es wird eine Methode zur Herstellung, Montierung und Färbung von epidermalen Häutchenpräparaten zur dreidimensionalen Analyse des Epidermis-Aufbaues beschrieben. Eine Kombination mit der Histoautoradiographie ist bedingt möglich. Damit ist eine Basis für weitere Untersuchungen mit neuer Fragestellung geschaffen. 2. Die untersuchten Objekte Mäuseohr und Rückenhaut zeigen eine bisher unbekannte, auffallende Musterbildung mit Zuordnung bestimmter interfollikulärer basaler Gruppen zu Zellen des Stratum spinosum und des Stratum corneum. Es handelt sich um rosettenähnliche Gebilde aus mehreren peripheren Basalzellen und meist einer zentralen Spinosumzelle. 3. Die Bedeutung der Befunde für die normale wie für die pathologische Regeneration und Neoplasie der Epidermis ist offen. Es werden Beziehungen zum Regenerationsmodus und damit zur Proliferationskinetik der Epidermis diskutiert. In diesem Zusammenhang sind die Untersuchungen auch für die Humanpathologie relevant.
    Notes: Summary 1. A method for the preparation, mounting, and staining of thin preparations of mouse epidermis is described, which allows a three-dimensional analysis of epidermal structure. A limited combination of this technique with histo-autoradiography is possible, thereby presenting a broader basis for further investigations. 2. Both ear and back skin show a striking, previously unrecognized pattern of organization, in which definite interfollicular basal cell groups are oriented towards cells of the stratum spinosum and stratum corneum. A rosettelike picture of many peripheral basal cells and, in most instances, a central spinosum cell is found. 3. The significance of our findings for normal and pathological regenerations as well as neoplasia of the epidermis remains open. We discuss relationships to the mode of regeneration and thus the kinetics of proliferation in the epidermis. Our investigations are also relevant for human pathology in this regard.
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  • 8
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    Cell & tissue research 148 (1974), S. 347-358 
    ISSN: 1432-0878
    Keywords: Pituitary, catfish ; Cell-types ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Seven distinct types of secretory cell can be differentiated in the pituitary gland of the fresh-water catfish, Heteropneustes fossilis. The presumptive prolactin cells appeared completely degranulated. Corticotrophs and thyrotrophs were identified by their response to metopirone and β methasol or to thiourea and thyroxine respectively. A single gonadotrophic cell type was identified, which showed a progressive increase in nuclear size, cell size and cytoplasmic granulation related to vitellogenesis in the ovary. Presumptive somatotrophs did not react to any of the experimental treatments used. In the pars intermedia, one of the two cell types — the PbH+ cells — showed an increase in activity in response to metopirone, thyroxine and reserpine; the PAS+ cells in this region remained relatively inactive.
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  • 9
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    Cell & tissue research 144 (1973), S. 573-583 
    ISSN: 1432-0878
    Keywords: Gills ; Tilapia mossambica ; Blood vessels ; Arteriovenous anastomoses ; Light microscopy ; Quantitative evaluation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The non-respiratory vascular system of T. mossambica gill filaments was studied in serial longitudinal and cross sections. Comparatively few scattered vascular communications occur between the afferent filament artery and the central venous sinus (AVAaff). The efferent filament artery, however, is connected by regularly arranged anastomoses (AVAeff), directly, and sometimes indirectly via nutritive vessels, to the central sinus. These AVAeff are about as numerous as lamellae counted on one side of each filament, although they diminish slightly in number towards the filament base. The relation AVAeff to AVAaff was 17.6:1 in the distal and 17.8:1 in the basal filamental region, while in the tip region of 7 filaments 126 AVAeff but only 1 AVAaff were encountered. No direct connection between the lamellar lacunae and the central sinus was detected. According to these results, non-respiratory intrafilamental blood shunting appears unlikely. AVAeff are assumed to be the main route for blood entering the central venous sinus which would consequently flow into the branchial veins.
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  • 10
    ISSN: 1432-0878
    Keywords: Entorhinal area ; Guinea pig ; Subdivision ; Distribution of heavy metal ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The boundary line dividing the entorhinal area into a pars medialis and a pars lateralis has been determined in adult guinea pig by comparison of graphic maps of the distribution of heavy metals and of the terminal field of a presubiculum → entorhinal area projection. The virtual coincidence of the histochemically and hodologically based subdivisions strongly supports the assumption that the obtained boundary line represents the true location of a functional limit between the two entorhinal parts.
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  • 11
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    Cell & tissue research 151 (1974), S. 219-228 
    ISSN: 1432-0878
    Keywords: Cell death ; Central nervous system ; Embryogenesis of Calliphora (Insecta) ; Vital staining ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary During the late embryonic period, cell death occurs in the central nervous system of Calliphora erythrocephala. The degenerating cells are small and rounded, contain a pyknotic nucleus, and selectively accumulate basic vital dyes such as nile blue sulphate and acridine orange. The number of degenerating cells is highest 13–15 hours after oviposition and decreases during the shortening of the ventral nerve cord. Nevertheless, these degenerating cells do not represent a morphogenetic phenomenon, since their number is too small to account for the 55% decrease in the length of the ventral nerve cord. In all probability the pyknoses seen in the central nervous system of this holometabolic insect are to be interpreted as phylogenetic degenerations of clearly vestigial neuroblasts that have lost functions they might have retained in the larvae of insects in lower orders, for instance the innervation of legs, wings, a larger number of abdominal segments, etc. About half of the pyknoses occur in pairs.
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  • 12
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    Cell & tissue research 141 (1973), S. 493-503 
    ISSN: 1432-0878
    Keywords: Pituitary ; Glomerular body ; Marine teleost ; Hexagrammos otakii ; Larval osmoregulation ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the larvae of the marine teleost, Hexagrammos otakii, the prolactin, ACTH, STH and pars-intermedia cells as well as the neurohypophysis and glomerular body showed no histological change at the time of hatching. These cells or organs do not seem to be specifically involved in the mechanisms of adjustment to the hypertonic osmotic stress at this time. The size of the nuclei of the prolactin cells increased in fresh-water (F. W.) and that of the ACTH cells decreased in 2/3, 1/2 and 1/3 sea-water (S.W.). The amount of chrome-alum haematoxylin-positive material in the neurohypophysis of fish immersed in 1/2 S.W., 1/3 S.W. and F.W. was less than that of the control fish. There was a difference in the glomerular ratio between the fish kept in F. W. and control fish. And the red blood cells in the glomerulus of the larvae kept in 1/2 and 1/3 S.W. apparently increased in number in comparison with the control fish.
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  • 13
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    Cell & tissue research 143 (1973), S. 535-547 
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Rat ; Mitoses, topographic distribution ; Influence of fixation ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les mitoses de l'adénohypophyse du rat mâle ont été examinées comparativement après fixation par perfusion et après fixation par immersion. Après fixation par perfusion la répartition topographique des mitoses dans le parenchyme est homogène, et l'index mitotique est de 1,85 pour 1 000 cellules. Après fixation par immersion, la densité des mitoses décroit de la périphérie vers le centre, et l'index mitotique n'est que de 0,80 mitoses pour 1 000 cellules. La lenteur de pénétration du fixateur pendant l'immersion est tenue pour principal responsable des différences observées. Elle entraîne une modification de l'aspect des mitoses, telle que plus de la moitié d'entre elles ne sont pas identifiables et échappent aux comptages.
    Notes: Summary The mitoses of the adenohypophysis in male rats have been observed comparatively after fixation by perfusion and fixation by immersion. After fixation by perfusion, the topographic distribution of mitoses throughout the parenchyma is homogenous and the mitotic index is 1.85 for 1 000 cells. After fixation by immersion, the density of the mitoses is decreasing from the periphery towards the center and the mitotic index is only 0.80 for 1 000 cells. The slow penetration of the fixative during fixation by immersion is considered to be the main cause in the differences observed. This slow action causes a modification in the aspect of the mitoses, and more than fifty percent of them cannot be identified and escape to the counts.
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  • 14
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    Cell & tissue research 147 (1974), S. 249-257 
    ISSN: 1432-0878
    Keywords: Chromosomes ; Action of colchicine ; Botryllus schlosseri (Ascidia) ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The course of c-mitosis in early embryos ofBotryllus schlosseri is arrested in a bizarre c-metaphase configuration in which the chromosome arms are tightly paired and sister kinetochores are well separated. This new type of c-metaphase chromosomes occurs because kinetochores are normally separated during prometaphase and sister c-chromatids tend to adhere to each other. Abnormal activity of sister kinetochores, chromosome fragmentation, adherence between chromosomes, overcondensation of chromosomes and bipolar arrangement of the c-pairs, are some additional effects induced by colchicine treatment of oocyte cleaving eggs ofB. schlosseri that have been studied. The behaviour of both normal and c-chromosomes ofB. schlosseri suggests that the forces acting upon kinetochores reach a minimum at metaphase and that they are not diminished by colchicine treatment.
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  • 15
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    Cell & tissue research 144 (1973), S. 353-368 
    ISSN: 1432-0878
    Keywords: Epidermis ; Bufo bufo ; Moulting cycle ; Structure ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Skin samples were taken from 17 toads (Bufo bufo) in various phases of the moulting cycle. The phase was determined by recording a number of moulting intervals prior to sacrifice and by study of the macroscopic appearance of the skin and the moulting behaviour at sacrifice. Eight of the toads were in the moult when the samples were taken. Characteristic morphological changes were found to be restricted to a period immediately prior to and after shedding of the slough. Chemical changes of the membranes of stratum corneum (or material adhered to them) were observed already prior to adoption of the moulting posture. The separation from the underlying epidermis prior to shedding was accompanied by a swelling of the stratum corneum cells. After final detachment of the slough the replacement layer was differentiated into a new stratum corneum within 24 hours. Significant changes in the morphology of the flask cells were not observed. The findings are discussed with emphasis on the processes of separation and differentiation of the stratum corneum. Based on the morphology of the epidermis the following terminology is proposed for the phases of the moulting cycle: Intermoult phase, preparation phase, early shedding phase, late shedding phase, and differentiation phase.
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  • 16
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    Cell & tissue research 150 (1974), S. 95-105 
    ISSN: 1432-0878
    Keywords: Mucosal mast cells ; Rat ; Compound 48/80 ; Degranulation, Release ; Light microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mast cells in the tongue and lamina propria of the duodenal mucosa, representing two cell types with different morphological, histochemical and functional properties have been studied under the electron microscope. Both cell types were found to contain similar moderately electron dense and homogeneous granules surrounded by a tight membrane constituting the basic ultrastructural characteristics of mast cells. The mucosal mast cells, however, contained fewer granules per cell and the individual granules often exhibited considerable variation in size. In addition the mucosal mast cells, unlike those of the tongue, peritoneum and skin, had a smooth plasma membrane lacking microvilli and possessed irregularly shaped or lobulated nuclei. In the tongue mast cells, administration of Compound 48/80 caused the formation of vacuoles around the granules, expulsion of granules from the cells and disintegration of the granular matrix, but no changes that could be attributed to 48/80 could be found in the mucosal mast cells.
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  • 17
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    Cell & tissue research 150 (1974), S. 213-229 
    ISSN: 1432-0878
    Keywords: Nerve cells ; Regeneration ; Neurosecretion ; Hydra viridis, littoralis ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using whole mount preparations, nerve cells at the cut surface (head region) and also the peduncular-basal disk region were studied during various stages of regeneration (zero hour — 96 h) in Hydra. Leucomethylene blue staining technique allows excellent stainability of nerve cells and thus a statistical count of them at the specified stages of regeneration was obtained. Within 1 h after transection a significant increase (P 〈 0.001) in release of neurosecretory droplets occurs. Between 4 and 15 h of regeneration the number of perikarya remains relatively constant, but the number of processes increases by 200%. This indicates that neurons in Hydra have the capacity to regenerate their processes. Their growth in length is dramatically illustrated at 18 hrs. of regeneration. This degree of anastomosing arrangement is not observed in any other stage of regeneration. After this time the majority of processes break down releasing the neurosecretory materials. A study of the number of perikarya, the number of neurites and the number of neurosecretory droplets in regenerating animals reveals a continuous increase in the number of nerve cells and neurosecretory droplets released for up to 24 h. With the accumulation of nerve cells at the cut surface (24 h of regeneration) there is a simultaneous appearance of tentacle outpushings. The tentacles increase in number and length during the subsequent periods of regeneration. As the regenerative process approaches completion (72–96 h) the number of neurosecretory droplets released decreases, approaching the pre-transection levels as seen in normal animals. It is suggested that the neurosecretory material may act as a “trophic” agent which stimulates differentiation of interstitial cells into nerve cells and thus influences the regenerative process.
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  • 18
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    Cell & tissue research 148 (1974), S. 277-286 
    ISSN: 1432-0878
    Keywords: Insect visual system ; Apis mellifera ; Retina-lamina projection ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The retina-lamina projection in the visual pathway of the bee was studied by the reduced silver and Golgi techniques. Two main types of visual cell axons (R-fibres) were found: (1) at least two forms of short visual fibres terminate at two levels in the lamina; (2) the long visual fibres cross the first optic chiasma and terminate at two different levels of higher order neurons in the medulla. Six short and three long visual fibres leave each retinula in the bee's eye. Whereas two types of short visual cells can be distinguished by the arborization patterns of Golgi-stained preparations, as well as by their fibre diameters, three different types of long visual fibres can be found. In each cartridge (“neuroommatidium”) the six short visual cells closely appose three monopolar cells (L-fibres, second order neurons). Thus each axon bundle crossing the first (or intermediate) chiasma contains at least six large argyrophilic fibres (three long visual cells and three monopolar cells), and these can be seen in cross-sections of reduced silver preparations. In addition, centrifugal fibres originating in the medulla and terminating in the lamina as well as amacrine (intrinsic) cells of the lamina have been resolved by Golgi impregnation.
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  • 19
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    Cell & tissue research 136 (1973), S. 45-58 
    ISSN: 1432-0878
    Keywords: Chicken ; Bursa Fabricii ; Thymus ; Spleen ; Tonsilla caecalis ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bursa Fabricii, Thymus, Milz und Zäkaltonsillen von 63 Hühnern im Alter von 1 Tag bis zu 1 Jahr werden histologisch untersucht, wobei das Vorkommen der als bursaabhängig geltenden Keimzentren und Plasmazellen quantitativ erfaßt wird. Beide treten in der Milz und Tonsilla caecalis erst während des Reifestadiums der Bursa in zunehmender Menge auf. Mit Beginn der Bursainvolution werden Keimzentren und pyroninophile Zellen in der Milz selten, während in den Zäkaltonsillen kaum Veränderungen auftreten. Im Thymus verschiebt sich das Verhältnis zwischen Mark- und Rindenbreite zugunsten des Marks. Bei 8 Monate alten Tieren fehlt die Rinde vollständig. Plasmazellen treten im Thymusmark während des Reifestadiums der Bursa auf und nehmen mit dem Beginn deren Involution zu. In dem Zeitraum zwischen 2 Wochen und 5 Monaten ändert sich das Verhältnis zwischen reifen und unreifen Plasmazellen beständig zugunsten der reifen Plasmazellen.
    Notes: Summary Bursa of Fabricius, thymus, spleen and cecal tonsils were histologically examined with special reference to the number of bursa-dependent germinal centers and plasma cells in 63 chickens, 1 day to 1 year old. Both structures appear in spleen and cecal tonsils increasingly during the bursal maturity stage. At the beginning bursal involution, germinal centers and pyroninophilic cells become rare in the spleen, in contrary no changes appear in the cecal tonsils. The medulla cortex ratio of the thymus changes in favour of the medulla, in animals 8 months of age the cortex is absent. Plasma cells appear in the medulla of the thymus during the bursal maturity stage and increase in number with beginning involution. During the period of 2 weeks to 5 months the proportion between mature and immature plasma cells changes in favour of mature cells.
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  • 20
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    Cell & tissue research 139 (1973), S. 149-170 
    ISSN: 1432-0878
    Keywords: Suctorial disc ; Echeneis naucrates ; Sustentacular tissue ; Sense organs ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die lichtmikroskopische Untersuchung der Haftscheibe des Teleostiers Echeneis naucrates führte unter anderen zu folgenden Ergebnissen: 1. Das mehrschichtige, Schleimzellen enthaltende Epithel der Scheibe weist deutliche regionale Unterschiede auf. So ist auf der Dorsalfläche der flexiblen Randlippe eine an Schleimdrüsen besonders reiche bandförmige Zone ausgebildet. Ferner werden die Eingänge in die Taschen zwischen den Lamellen des Saugapparates und die Oberfläche der medianen Hautleiste von stark schleimbildendem Epithel umgeben bzw. bedeckt. Der Schleimstreifen der Haftscheibe wird als Einrichtung zur Abdichtung der Haftfläche gedeutet. 2. Im Drüsenfeld auf der Dorsalfläche der Randlippe liegen große Sinnesorgane vom Typus der Chemorezeptoren. Ihre Funktion besteht möglicherweise in der Prüfung der Oberfläche des Wirtes, an dem sich Echeneis festsaugt. Die Angaben von Houy (1910), wonach diese Sinnesorgane in der Wand eines Kanalsystems liegen und nicht intraepithelial, beruhen auf der Fehlinterpretation artifiziell entstandener Spalten. 3. Die interlamellären Taschen der Saugplatte werden von einem verhältnismäßig niedrigen und an Schleimzellen armen, gefältelten Epithel ausgekleidet. Es ist der Beanspruchung auf Dehnung angepaßt, die bei dem Ansaugen infolge Vertikalstellung der Lamellen der Scheibe auftritt. 4. Der ovale abgeplattete Stützgewebsstreifen innerhalb der Randlippe, dessen Gewebe von Houy (1910) nicht klassifiziert werden konnte, besteht aus hyalinzelligem Chondroidgewebe (Schaffer), das von kollagenen und elastischen Fasern, ferner von einem weitmaschigen Kapillarnetz durchsetzt wird. Zarte Septen aus Kollagenfilamenten umgeben jede einzelne Hyalinzelle. Das im Zentrum des Reifens gelegene univakuoläre Fettgewebe differenziert sich aus den Hyalinzellen des Chondroidgewebes, die ihrerseits von perichondralen Fibrozyten abstammen. Auch die innerhalb der Lamellen des Saugapparates gelegenen Knorpelformationen werden von hyalinzelligem Chondroidgewebe gebildet, das mit Fettgewebe in Zusammenhang steht. 5. Die Faserarchitektur des Randstreifens entspricht der trajektoriellen Bauweise des Hyalinknorpels.
    Notes: Summary A light microscopical study of the suctorial disc of the teleost Echeneis naucrates revealed the following details: 1. The stratified mucous-cell bearing epithelium of the disc shows distinctive regional differences. Thus the dorsal surface of the flexible marginal lip contains a peculiar glandular zone which is particularly rich in mucous cells. Furthermore, the openings to the pockets between the lamellae of the suction apparatus and the surface of the median dermal ridge are mucus producing zones. These areas are interpreted as devices for tightening. 2. In the mucous zone of the dorsal surface of the marginal lip are large sensory organs of chemoreceptor type. Possibly their function consists in the chemical assessment of the host's surface. The statement by Houy (1910) according to which these organs are located in the wall of a canalicular system rather than intraepithelially stems from his erroneous interpretation of artifactitious clefts. 3. The interlamellar pockets of the suction disc are lined by a low folded epithelium with relatively few mucous cells. It is capable of adjusting to the stretch caused by the tilting of the disc's lamellae during suction. 4. The oval flattened ring of sustentacular tissue within the marginal lip, which Houy (1910) was unable to classify, represents a chondroid tissue consisting mainly of hyaline cells (“hyalinzelliges Chondroidgewebe, Schaffer 1930”) and traversed by collagenous and elastic fibres. It also contains a wide-meshed capillary network. Each hyaline cell is surrounded by delicate septa formed by collagenous filaments. The unilocular adipose tissue in the ring's center develops from the hyaline cells of the chondroid tissue which in turn are derived from perichondrial fibrocytes. Also the cartilaginous structures within the lamellae of the suction apparatus are formed by the same chondroid tissue that is in connection with adipose tissue. 5. The spatial arrangement of the fibres and fibrils traversing the sustentacular tissue within the marginal lip corresponds to the trajectorial pattern of hyaline cartilage.
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  • 21
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    Cell & tissue research 137 (1973), S. 313-329 
    ISSN: 1432-0878
    Keywords: Nervous system ; Limulus polyphemus ; Abdominal ganglion ; Neurons ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology of the first abdominal ganglion of Limulus polyphemus (L.) was examined by reconstructions from stained serial sections. This ganglion, one of a chain of eight which form the ventral cord, is bilaterally symmetrical and shows the typical invertebrate division into two zones, cellular rind and fibrous core. The core of the ganglion comprises two major longitudinal tracts which are continuous with the connectives between the ganglia and four areas of regional neuropile which occur at the roots of the nerves developed from the ganglion. Minor tracts and neuropile are present. The rind is divided into several cell groups: U-shaped lobes around the nerve roots, a lateral lobe between the nerve roots and two cell layers along the ventral aspect. Six cell types are recognized: encapsulated unipolar cells, secretory unipolar cells (two types), encapsulated bipolar cells, “neuroglandular” bipolar cells and small non-encapsulated cells.
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  • 22
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    Cell & tissue research 140 (1973), S. 509-534 
    ISSN: 1432-0878
    Keywords: Genital tract ; Viviparous toad ; Cyclic variations ; Light microscopy ; Biometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'oviducte de Nectophrynoïdes occidentalis Angel, petit crapaud vivipare d'Afrique occidentale, peut être divisé en quatre parties: le pavillon, la trompe, l'utérus et le tronc commun utérin distal. Seul le pavillon ne varie pas au cours de l'année; il reste toujours transparent et dépourvu de cellules glandulaires. Un épithélium cilié tapisse sa lumière. La trompe est un conduit rectiligne de 7 à 10 millimètres de long qui peut être divisé en quatre segments principaux, différenciés par la constitution chimique des substances de la muqueuse. La structure de la trompe est relativement simple et comparable à celle des autres Vertébrés inférieurs. Elle apparait alternativement sous deux aspects principaux: trompe au repos et trompe active. La période de repos se poursuit pendant une bonne partie de la gestation; la reprise d'activité sécrétrice s'effectue plusieurs semaines avant la parturition et atteint son maximum dans les jours qui précèdent l'ovulation. L'utérus subit continuellement des remaniements. Tour à tour vide et gravide, il passe par trois phases essentielles: une phase de nécrose s'étendant sur les douze jours qui suivent la parturition et correspondant à l'élimination de la couche superficielle de la muqueuse; une phase de prolifération, allant du douzième jour après la mise-bas jusqu'à lóvulation suivante; caractérisée par le régénération progressive de la muqueuse à partir de la couche profonde restée en dehors des processus de nécrose; une phase de sécrétion et d'hypérémie qui dure pendant toute la gestation. La partie terminale de l'oviducte, formée par la confluence des deux conduits müllériens, subit les mêmes variations que l'utérus gestant. Son épithélium est tout-à-fait comparable à celui de l'utérus; son chorion, en revanche, est beaucoup plus dense et développé mais ne possède qu'une faible vascularisation.
    Notes: Summary The oviduct of Nectophrynoides occidentalis Angel, a small viviparous toad of West Africa, is divided into four parts: ostium, tube, uterus and the distal common uterine segment. Only the ostium does not show seasonal variations: it always remains transparent and is devoid of glandular cells. A ciliated epithelium lines its lumen. The tube is rectilinear, about 7–10 millimeters long and may be subdivided into four principal segments, on the basis of the chemical composition of the substances of the mucosa. Its structure is relatively simple and comparable to that of other lower vertebrates. Its appearance differs according to whether it is at rest or in activity. For most of the gestation period the tube is at rest but secretory activity starts several weeks before birth. It reaches its maximum during the days immediately preceding ovulation. Unlike the tube, the uterus undergoes continuous changes, which can be pregnant, divided into three essential phases: a phase of necrosis, extending to twelve days following birth, and corresponding to the elimination of the superficial layer of the mucosa; a phase of proliferation, extending from the twelfth day after birth until the subsequent ovulation, characterized by the progressive regeneration of the mucosa, which is initiated by the deep layer which was not involved in necrosis; a phase of secretion and hyperemia which lasts during the whole period of gestation. The terminal part of the oviduct, formed by the confluence of the two Müllerian ducts, undergoes the same variations as the uterus, its epithelium being completely comparable to that of the uterus; its chorion, however, is much denser and better developed but its vascularization is less intense.
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  • 23
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    Cell & tissue research 146 (1973), S. 1-14 
    ISSN: 1432-0878
    Keywords: Ventral cord ganglia ; Limulus polyphemus ; Cell types ; Neurosecretion ; Cytochemistry ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ventral cord ganglia of the horseshoe crab, Limulus polyphemus, contains six distinct cell types: three appear to be ordinary neurons and three exhibit the staining affinities of neurosecretory cells. The presumed neurosecretory cells have been termed neurosecretory cell I (NSC I), NSC II and NSC III. NSC I cells contain a colloid-like inclusion which may occur as a single small vacuole or occupy more than one-half of the cell volume. Colloid inclusions occur with greater frequency toward the periphery, although small cells of similar staining affinity occur in cords extending to the fibrous core. The histochemical tests suggest that the cytoplasm is positive for proteins, but contains no strong acidic groups which may have been derived from S-S or S-H groups. The presence of carbohydrate is also indicated. NSC II cells exhibit distinct secretory cycles. Early in the cycle the cytoplasm becomes phloxinophilic and progresses to a distinct fuchsinophilic stage. Small homogeneous irregular inclusions are found in the axon hillock during the latter stages of the cycle. Histochemical tests suggest the presence of a carbohydrate and strong acidic groups which may have been derived from S-S or S-H groups. There are small cells present which appear to be immature neurosecretory cells. NSC III cells are characterized by a perinuclear ring of cytoplasm which is stained by chrome alum hematoxylin but not by paraldehyde fuchsin. A secretory cycle may also be present in this cell type. The three cell types presumed to be ordinary neurons exhibit no particular staining affinity for the stains or tests used in this study.
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  • 24
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    Cell & tissue research 146 (1973), S. 261-279 
    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Rat, Cat ; Motor endplate ; Reinnervation ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The formation of endplates outside the original endplate region of a muscle fibre was studied in slow and fast rat muscles. It was found that such new endplates are readily formed on the soleus muscle, whereas hardly at all in the fast extensor digitorum longus. Most new endplates appear to be morphologically normal within 2 months after nerve implantation. 2. The time course of recovery of slow and fast cat muscles was followed after crushing the sciatic nerve. It was found that the slow soleus muscle recovers more rapidly than the fast flexor hallucis longus muscle. 3. The endplates of reinnervated cat muscles are more complicated than those of the control muscles, but have nevertheless fewer nerve terminals per endplate. Reinnervated muscles are more sensitive to curare and it is suggested that this is due to a decrease in transmitter release, for it was found that less acetylcholine is released from reinnervated rat hemidiaphragms than from control ones. 4. Motor and sensory reinnervation of spindles and tendon organs was studied. At the time when motor reinnervation is almost completed the sensory endings from spindles and tendon organs are highly abnormal. Thus sensory reinnervation proceeds much more slowly than motor.
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  • 25
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    Cell & tissue research 147 (1974), S. 505-527 
    ISSN: 1432-0878
    Keywords: Cartilage ; Ageing ; Chondrocyte and matrix ; Light microscopy ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histological, histochemical, and ultrastructural investigations have been carried out on ageing costal and tracheal cartilage of rats. The following age groups of animals have been studied: 1, 7, 14, 20, 30, 45, 75 days, 6 months, and 2 years. Ageing induces cellular changes which are represented by a reduction in the number of chondrocytes, a progressive increase in glycogen deposition, and processes of degeneration, the most frequent of which is the accumulation of lipidic material within large cytoplasmic vacuoles. Changes in the intercellular matrix become evident after 20 days in costal cartilage and after 30 days in tracheal cartilage. Chondroitin sulphate decreases while keratan sulphate, whose presence is limited to the territorial matrix, increases. Glycoproteins increase slightly in young animals and then remain constant; they decrease in the subperichondrial areas in old animals. Ultrastructurally, the matrix of cartilage of young animals contains thin collagen fibrils, most of which have no periodic banding. Roundish electron dense granules are associated with these fibrils. Irregular filaments associated with small electron-dense circular bodies are present around chondrocytes as well as within cytoplasmic vacuoles. With increasing age, and coincident with the reduction of chondroitin sulphate, the thickness of collagen fibrils increases, their period becomes evident, and the associated matrix granules decrease in number and size. Areas containing these fibrils undergo calcification, which frequently starts within roundish bodies of cellular origin. Collagen fibrils with a period of 640 Å but a highly variable thickness are often present in cartilage of adult and old rats. These fibrils seem to be due to an abnormal synthetic activity of chondrocytes.
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  • 26
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    Cell & tissue research 147 (1974), S. 537-549 
    ISSN: 1432-0878
    Keywords: Insect haemocytes ; Classification ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A simplified insect haemocyte classification has been formulated by a light microscopic examination of the haemolymph of insects from fifteen Orders. Six cell types or developmental stages can be distinguished: (1) Prohaemocytes, (2) Plasmatocytes, (3) Granular Cells, (4) Spherule Cells, (5) Cystocytes, and (6) Oenocytoids. The structure and occurrence of these haemocytes are described together with the structural variations which occur in each cell type. Due to considerable overlap in structure and the presence of numerous intermediates the six cell types may represent different developmental and/or functional stages of one basic cell type. The available evidence for this unitarian hypothesis is discussed.
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  • 27
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    Cell & tissue research 149 (1974), S. 267-274 
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Teleostei ; Euryhalinity ; Eta cells ; Dilatation of capillaries ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Eta cells of the pituitary of Aphanius dispar specimens adapted to sea water are densely packed and have folded nuclei. Unfolding of the nuclei accompanied by cellular hypertrophy and hyperplasia was observed 24 hours after experimental transfer of specimens into fresh water. In specimens from natural habitats and adapted over long periods to fresh water, the nuclei are rounded and cellular hypertrophy attains maximal values. Dilatation of capillaries in the hypophysis was observed in about one third of the specimens within the first few days after transfer to fresh water. In specimens adapted either to fresh water or to sea water the capillaries were normal. Epsilon cells from seawater specimens have elongated nuclei perpendicular to the basement membrane which separates the rostral neurohypophysis from the adenohypophysis; in freshwater specimens epsilon cell nuclei are rounded.
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  • 28
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    Cell & tissue research 151 (1974), S. 343-346 
    ISSN: 1432-0878
    Keywords: Cerebellar cortex ; Rhesus monkey, cat ; Basket cell axons ; Regional differences ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the cerebellar cortex of rhesus monkey and cat differences in the arrangement of basket cell axons and dendrites are described. In the anterior lobe, basket cell axons are short, very small in number and of a small diameter. In the posterior and nodulofloccular lobe, these fibers form plexus-like accumulations within the lower third of the molecular layer. In these parts they are longer, more numerous and bigger in diameter. Finally the contribution of Lugaro cell dendrites to this fiber accumulation is discussed.
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