ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Histochemistry  (14)
  • Springer  (14)
  • Nature Publishing Group
  • 1970-1974  (14)
  • 1950-1954
  • 1935-1939
  • 1970  (14)
Collection
Publisher
  • Springer  (14)
  • Nature Publishing Group
Years
  • 1970-1974  (14)
  • 1950-1954
  • 1935-1939
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 5 (1970), S. 170-182 
    ISSN: 1432-0827
    Keywords: Cartilage ; Bone ; Gonadectomy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des coupes de tibias de rats mâles et femelles, gonadectomisés et témoins, sont soumises à divers examens histologiques et histochimiques pour étudier les effets de la gonadectomie au niveau du tissu osseux. Le cartilage épiphysaire proximal des rats castrés et des rattes, ayant subi l'ablation des ovaires, est oblitéré par de l'os 5 mois après l'intervention. En outre, la diaphyse présente une ostéoporose. La matrice intercellulaire du cartilage épiphysaire des rats gonadectomisés est fortement PAS positive, contrairement à la réaction fortement alcian positive de la matrice des animaux témoins. Les travées diaphysaires des rats gonadectomisés se colorent en rouge avec le PAS et contiennent des régions étendues d'ilôts cartilagineux calcifiés. En outre, le cartilage épiphysaire et les travées osseuses de ces animaux se colorent faiblement à la ninhydrine-Schiff. Les réactions colorées suggèrent que la gonadectomie induit une altération dans le degré d'agrégation protéino-mucopolysaccharidique de la matrice organique; ce qui interfère probablement avec la minéralisation osseuse.
    Abstract: Zusammenfassung Tibiaschnitte von unbehandelten und kastrierten männlichen und weiblichen Ratten wurden mit verschiedenen histologischen und histochemischen Techniken untersucht, um die Knochenveränderungen im Anschluß an die Gonadektomie zu bestimmen. Es wurde beobachtet, daß sowohl beim kastrierten Männchen wie beim ovarektomierten Weibchen der proximale Epiphysenknorpel nach dem fünften postoperativen Monat durch Knochen „eingekapselt” ist. Dazu wurde auch eine Osteoporese des Knochenschaftes beobachtet. Der Epiphysenknorpel der kastrierten Ratten zeigte eine intensiv PAS-positive interzelluläre Matrix, im Gegensatz zu der alcianophil reagierenden Matrix von Kontrolltieren. Die Diaphysentrabekel von kastrierten Ratten färbten sich mit PAS rot und enthielten große Bezirke von mineralisierten Knorpelkernen. Dazu färbten sich der Epiphysenknorpel und die Trabekel dieser Tiere nur schwach mit der Ninhydrin-Schiff-Reaktion. Die Resultate der verschiedenen Färbungen zeigten, daß nach Gonadektomie eine Veränderung im Aggregatzustand der Protein-Mucopolysaccharidkomponente der organischen Matrix stattfindet, was zu einer Interferenz im Knochenmineralisationsprozeß führen könnte.
    Notes: Abstract Sections of the tibias from both intact and gonadectomized male and female rats were subjected to various histological and histochemical methods to determine the changes in the bone following gonadectomy. It was observed that both in castrated males and in ovariectomized females, the proximal epiphyseal cartilage became “sealed off” by bone after the fifth post-surgical month. In addition osteoporosis of the shaft of the bone was also observed. The epiphyseal cartilage of the gonadectomized rats exhibited intense PAS positive intercellular matrix in contrast to the alcianophilic ature of the matrix from control animals. The diaphyseal trabeculae from gonadectomized rats stained red with PAS and contained wide regions of calcified cartilage cores. In addition, the epiphyseal cartilage and bony trabeculae from these animals stained faintly in the ninhydrin-Schiff reaction. The staining reactions indicated that in gonadectomy there was an alteration in the state of aggregation of protein-mucopolysaccharide component of the organic matrix, a situation which would result in an interference in the mineralization of the bone.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 6 (1970), S. 316-328 
    ISSN: 1432-0827
    Keywords: Epiphysis ; Cartilage ; Bone ; Parathyroidectomy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des coupes de tibias d'animaux entiers et parathyroidectomisés sont étudiées histologiquement et histochimiquement pour préciser les effets de la parathyroidectomie sur l'os. Les épiphyses cartilagineuses proximales montrent uneaugmentation relative de matrice intercellulaire, une diminution en cellules, et une désorganisation de l'arrangement cellulaire. Le cartilage épiphysaire des rats parathyroidectomisés montre une matrice intercellulaire intensément PAS positive, contrairement aux affinités à l'alcian de la matrice des animaux témoins. Les travées diaphysaires des animaux parathyroidectomisés se colorent en rouge au PAS et contiennent de nombreux ilôts étendus de cartilage calcifié. En outre, le cartilage épiphysaire et les travées osseuses des animaux parathyroidectomisés se colorent moins intensément à la réaction de ninhydrine—Schiff que l'os des témoins. L'étude par les diverses colorations histochimiques, après parathyroidectomie, indique une altération des constituants collagène-protéine et mucopolysaccharide. Ces changements sont susceptibles de produire un trouble de la calcification de l'os.
    Abstract: Zusammenfassung Tibiaschnitte von Kontrollratten und von parathyreoidektomierten Ratten wurden histologisch und histochemisch untersucht, um die nach Parathyreoidektomie aufgetretenen Knochenveränderungen festzustellen. Der proximale Epiphysenknorpel von parathyreoidektomierten Ratten zeigte einerelative Zunahme der intracellulären Matrix, eine Abnahme des Zellgehaltes und eine Veränderung der zellulären Anordnung. Die Epiphysenknorpel der parathyeoidektomierten Ratten wiesen eine intensiv PAS-positive intracelluläre Matrix auf, im Gegensatz zur alcianophilen Reaktion der Matrix der Kontrolltiere. Die Diaphysen-Trabekel der parathyreoidektomierten Tiere ließen sich mit PAS rot färben und enthielten breite Zonen von verkalkten knorpeligen Kernen. Ferner ließen sich die Epiphysenknorpel und die Knochen-Trabekel von parathyreoidektomierten Tieren im Vergleich mit den Kontrolltieren weniger intensiv mit der Ninhydrin-Schiff-Reaktion färben. Die Resultate der verschiedenen histochemischen Reaktionen zeigten, daß die Parathyreoidektomie eine Veränderung der Protein-Mucopolysaccharide des Kollagens zur Folge hat. Diese neuen Bedingungen könnten eine Veränderung der Knochenverkalkung hervorrufen.
    Notes: Abstract Sections of the tibias from intact and from aparathyroid animals were examined histologically and histochemically to determine the changes in bone following parathyroidectomy. The proximal epiphyseal cartilages of parathyroidectomized rats showed arelative increase of intercellular matrix, decrease in cellularity, and disorganization of the cellular arrangement. The epiphyseal cartilages of the aparathyroid rats exhibited an intense PAS-positive intercellular matrix in contrast to the alcianophilic nature of the matrix from control animals. The diaphyseal trabeculae from aparathyroid animals stained red with PAS and contained wide regions of calcified cartilaginous cores. In addition, the epiphyseal cartilages and bone trabeculae from the parathyroidectomized animals stained less intensely to the ninhydrin-Schiff reaction than did the bone from the intact animal. The response to the various histochemical reactions indicated that following parathyroidectomy there was a change in the collagen-protein mucopolysaccharide components. This condition would produce an alteration in the calcification of bone.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 108 (1970), S. 582-596 
    ISSN: 1432-0878
    Keywords: Glandula bulbourethralis ; Goat ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Mehrzahl der sezernierenden Zylinderzellen in den tubulösen Endstücken der Glandula bulbourethralis der Ziege sind Schleimzellen. Ihre großen, aufgehellten Sekretgranula, die fast den gesamten Zelleib ausfällen, liegen so dicht, daß sie sich gegenseitig abplatten. Einzelne von ihnen haben ihre Hüllmembran verloren und neigen zur Konfluenz. Alle Schleimkörnchen sind PAS-positiv, viele von ihnen zeigen eine neuraminidaselabile Alcianophilie bei pH 2,5. Neben den Schleimzellen findet man in der Glandula bulbourethralis der Ziege einen zweiten Zelltyp, der durch helle, blasenförmige Kerne, ein stark entfaltetes granuläres endoplasmatisches Retikulum mit dilatierten Zisternen sowie ein ausgedehntes supranukleares Golgi-Feld gekennzeichnet ist. Dieser zweite Zelltyp enthält sehr elektronendichte, isoliert liegende Sekretkugeln, welche lichtmikroskopisch eine Proteinreaktion geben. Zwischen beiden Zelltypen kommen morphologische Übergangsformen vor. Dies macht es wahrscheinlich, daß es sich bei den beiden Typen lediglich um Funktionsstadien einer einzigen Drüsenzelle handelt. Die sezernierende Oberfläche beider Zelltypen ist durch die Ausbildung interzellulärer Sekretkapillaren vergrößert. Diese sind bereits lichtmikroskopisch aufgrund ihrer kräftigen ATPase- und 5′-Nucleotidaseaktivität zu identifizieren. Das gesamte Drüsenparenchym reagiert sehr stark auf unspezifische Esterase und deutlich positiv auf β-D-Galactosidase, β-D-Glucuronidase, Leucinaminopeptidase, Cytochromoxydase und Succinatdehydrogenase. Die letzten 5 Enzyme sind in den Schleimzellen in geringerer Konzentration festzustellen als in den Zellen mit Eiweißgranula.
    Notes: Summary The majority of the secretory cells in the tubular endpieces of the caprine bulbourethral gland are mucous cells. Their closely packed, relatively large secretory granules exhibit a low electron density. Some granules have lost their limiting membrane, this results in the accumulation of irregularly outlined masses of secretory material. All mucous secretory granules are PAS-positive, many of them are characterized by an alcianophilia at pH 2.5 which is extinguished by pre-treatment with a neuraminidase solution. The second type of secretory bulbourethral cell exhibits light, spherical nuclei, a well developed rough endoplasmic reticulum with dilated cisterns and a large supranuclear Golgi-Complex. The cytoplasm contains smaller, highly electron dense granules which are — according to histochemical tests — of protein nature. The existence of transitional forms between both described cell types permits the conclusion that they must be regarded as functional stages of one common gland cell. The secretory surface of both cell types is increased by intercellular canaliculi which can be identified in the light microscope by their strong ATPase and 5′-nucleotidase activities. The entire parenchyma of the gland is site of an exceptionally high esterase concentration. Furthermore, the gland cells contain considerable amounts of β-D-galactosidase, β-D-glucuronidase, leucine aminopeptidase, cytochrome oxydase and succinic dehydrogenase. These last five enzymes are histochemically more active in the protein secreting than in the mucus producing cell type.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 9-26 
    ISSN: 1432-0878
    Keywords: Nucleus lateralis tuberis ; Gillichthys ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nucleus lateralis tuberis (NLT) system of the teleost fish Gillichthys mirabilis was studied with respect to the anatomical organization and distribution of the neurosecretory neurons and the nature of their secretory material. NLT neurons occur in several different areas in the tuberal region of the hypothalamus, located immediately above the pituitary gland. Only the lateral and rostral NLT neurons show definite secretory activity. These neurons, generally of large size, contain numerous large granulated vesicles (LGV), 900–1,000 Å in diameter; on the other hand, the medial and ventrolateral neurons, forming a single layer of cells, are of small size and do not appear to be secretory. LGV are positive after ethanol-phosphotungstic acid (E-PTA) and zinc iodide-osmium tetroxide (ZIO) impregnations. Lateral and rostral neurosecretory neurons also show strong yellow-to-green specific fluorescence after the Falck-Hillarp technique, a strong indication that LGV are the site of catecholamine storage.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 22-50 
    ISSN: 1432-0878
    Keywords: Noradrenaline ; Fluorescence ; Bird ; Adrenergic Nerves ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenergic nervous system of the domestic fowl has been investigated using the Falck-Hillarp technique for the localisation of biogenic amines. The predominating catecholamine in adrenergic nerves is noradrenaline. In the fowl, adrenergic nerves are more widely distributed than in mammals, although comparable in appearance; clumps of adrenergic ganglion cells are present throughout the viscera, particularly in the abdomen. The heart and arterial vasculature has an adrenergic innervation comparable to that seen in mammals, but the veins are frequently more densely innervated, suggesting that they may play a more active role in the regional distribution of blood and in venous return than in mammals. The respiratory system of birds shows certain peculiarities and these are reflected in the adrenergic innervation of this system. The density of the adrenergic innervation of the genital organs is dependent on age; in males the innervation of the genital ducts only shows a marked development at sexual maturity, but in females it appears to be established before hatching. These findings are discussed in terms of the function of adrenergic nerves in the different situations.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 523-538 
    ISSN: 1432-0878
    Keywords: Ependyma ; Intracytoplasmic lipid bodies ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei erwachsenen Feuersalamandern und bei Salamanderlarven konnten in Ependymzellen sowie in subependymären Zellelementen des Gehirns und Rückenmarks zahlreiche rundliche intrazytoplasmatische Körper von starker Osmiophilie beobachtet werden, deren Durchmesser bis zu 12 μ betrug. Vereinzelt fanden sich diese voluminösen Gebilde auch im Cytoplasma von Satellitenzellen der Hirnnerven- und Spinalganglien. Das histochemische Verhalten und das Ultrastrukturbild der intrazytoplasmatischen Körper sprechen dafür, daß sie hauptsächlich aus Lipiden bestehen. Ihre funktionelle Bedeutung konnte bisher nicht eindeutig geklärt werden.
    Notes: Summary The ependymal and subependymal cells of the ventricular system and the central canal in adult und larval salamanders contain numerous unusually large intracytoplasmic osmiophilic spherical bodies with a diameter of up to 12 μ. Sporadically the bodies are found within satellite cells of peripheral ganglia. Histochemical and ultrastructural examination suggests that the bodies consist mainly of lipids. Their functional significance is unknown.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1432-0878
    Keywords: Isopod hindgut ; Histology ; Histochemistry ; Ultrastructure ; Osmo-regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The tubular hindgut of the intertidal herbivorous isopod, Dynamene bidentata, consists of a long dorso-ventrally flattened anterior region, surrounded by a network of muscles, and a short muscular sphincter which grades into a pair of anal flaps. The monolayer of epithelial cells forming the wall of the hindgut appears to take no part in the production of digestive enzymes, food absorption, or glycogen and lipid storage. One function of the hindgut is to propel undigested food material, enclosed within a peritrophic membrane, to the sphincter and anal flaps where faecal pellets are formed and ejected. At the fine structural level lateral plasma membranes, often partially obliterated by microtubules, are visible. The basal plasma membrane of a typical epithelial cell is deeply infolded, associated with mitochondria, and pinocytotic. The apical plasma membrane is irregularly folded, engaged in pinocytosis, and often encloses subcuticular spaces containing an acid mucopolysaccharide substance. An intima, composed of a thin double-layered epicuticle, and a thick acid mucopolysaccharide-positive endocuticle, overlies the cells. The endocuticle may selectively bind substances to the apical plasma membrane before they are engulfed by pinocytosis. The cells resemble those of osmoregulatory organs and may help counterbalance changes in the haemolymph concentration resulting from the intertidal existence of this isopod.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 83-100 
    ISSN: 1432-0878
    Keywords: Pineal gland and habenula (Ferret) ; Cervical sympathectomy (ganglionectomy) ; Monoamines ; Acetyl-cholinesterase ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A histochemical method for demonstrating amines by fluorescence showed that the pinealocytes of the ferret contained a high concentration of a yellow fluorophore (probably 5-HT). Numerous green-fluorescent (noradrenaline-containing) nerve fibres occurred around intrapineal blood vessels, between pinealocytes and in the N. conarii (which entered the gland caudally). A collection of neuron-like cells (the pineal ganglion) lay, surrounded by a meshwork of nerve fibres, in the posterior part of the pineal. Neither the cells nor the fibres of the pineal ganglion contained monoamines, but both showed the presence of acetyl-cholinesterase which otherwise was found in the pineal only in fibres which stretched from the ganglion towards the cranial pole of the gland. The medial habenular nucleus showed a remarkable perivascular green fluorescence not seen in the lateral habenular nucleus nor anywhere else in the adjacent diencephalon and brain stem. The cells and fibres of this nucleus also contained much acetyl-cholinesterase. Bilateral superior cervical ganglionectomy, or treating animals with reserpine, removed the green fluorescence from both pineal nerve fibres and the habenula. Ganglionectomy also resulted in a progressive alteration in the colour of the parenchymal fluorescence from yellow to green; the original yellow colour was restored by treating ganglionectomised animals with nialamide (a monoamine oxidase inhibitor). L-Dopa, 5-hydroxytryptophan or nialamide, alone or in combination, had no effect on the fluorescence of the nerve fibres or cells of the pineal, or on the habenula. These results are related to previous findings that pinealectomy or ganglionectomy prevents the acceleration by artificial light of oestrus in ferrets.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 1432-0878
    Keywords: ATPases ; Endometrial epithelium ; Histochemistry ; Ultrastructure ; Goat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Oberflächen- und Drüsenepithel des nicht graviden Uterus der Ziege wurden licht- und elektronenoptische Untersuchungen über den Nachweis der Mg++-, Ca++- und der (Mg++-Na+-K+)-aktivierbaren ATPasen durchgeführt. Spezifische Enzymaktivitäten lassen sich nur an Proben aus der Follikelphase nachweisen. In der Corpus luteum-Phase dagegen liegen diese Enzyme nicht in nachweisbarer Intensität vor. Die lichtmikroskopisch an den seitlichen Zellflächen nachgewiesenen Niederschläge der ATPasen finden sich elektronenoptisch als schwarze Granula an der Außenlamelle der lateralen Plasmalemm-Abschnitte. An apikalen und basalen Zellmembran-Bereichen in beiden Zyklusphasen zu beobachtende Reaktionsprodukte sind das Ergebnis einer Spontanhydrolyse des Substrats ATP bzw. von Spaltungen aufgrund der unspezifischen alkalischen Phosphatase. Haftkomplexe und Desmosomen sind regelmäßig frei von Bleisalz-Niederschlägen. Es können weder regionale Unterschiede in der Reaktionsintensität der Enzyme noch Differenzen im Hinblick auf die Verteilung und ultrastrukturelle Lokalisation von Niederschlägen der Mg++-bzw. der Ca++-aktivierbaren ATPase festgestellt werden.
    Notes: Summary Light and electron microscopic studies were performed on the Mg++-, Ca++-, and the (Mg++-Na+-K+)-activated ATPases in the surface and glandular epithelium of the non-pregnant uterus of the goat. Specific enzyme activities have been shown only in the epithelial samples collected during the follicular phase. In the luteal phase, none of these enzymes have been demonstrated with certainty. Precipitates of the ATPases which have been shown with the light microscope on the sides of the cell surface, can electron optically be observed as black granules on the outer lamella of the lateral plasmalemma. Reaction products on the apical and basal cell membrane regions observable in both cyclic phases, result from spontaneous hydrolysis of the substrate ATP, i.e. splitting caused by non-specific alkaline phosphatase. Junctional complexes and desmosomes are regularly free from lead salt precipitates. Neither regional differences in reaction intensity of the enzymes nor differences in the distribution and ultrastructural localization of either Mg++-or Ca++-activated ATPase precipitates can be observed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 496-516 
    ISSN: 1432-0878
    Keywords: Nucleus lateralis tuberis ; Innervation of the pituitary ; Gillichthys ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the pituitary gland of the teleost fish Gillichthys mirabilis was studied with light and electron microscopy in order to determine its nature and distribution. Two types of neurosecretory fibers (“A” and “B”) are present in the adenohypophysis. Type “A” fibers containing elementary neurosecretory granules, 1,500–1,600 Å in diameter, perforate the basement membrane in the neurointermediate lobe and make synaptoid contacts with MSH cells. Type “B” fibers containing large granulated vesicles (LGV), 900–1,000 Å in diameter, perforate the basement membrane in all lobes of the pituitary gland and terminate in direct contact with the several adenohypophysial cell types. At the electron-microscope level, LGV from type “B” fibers show a positive reaction to zinc iodide-osmium tetroxide (ZIO) impregnation. Reserpine treatment resulted in a depletion of their dense cores if osmium tetroxide was used as the only fixative, whereas double fixation with aldehydes and osmium tetroxide did not reveal appreciable changes. Yellow-to-green fluorescent fibers were detected in the pituitary gland after the Falck-Hillarp technique at sites corresponding approximately to the location of type “B” fibers, strongly suggesting the monoaminergic nature of the latter. After hypophysectomy, medial and lateral neurons of the nucleus lateralis tuberis (NLT) undergo retrograde degeneration. This finding, together with the morphological and cytochemical similarities of the LGV in NLT neurons and those in the type “B” fibers, suggests that the fibers originate from certain NLT neurosecretory neurons.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 51-63 
    ISSN: 1432-0878
    Keywords: Purkinje cell ; Golgi apparatus ; Dendrites ; Differentiation ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology of postnatal differentiation of the Golgi apparatus, the nucleus, the perikaryon, and the dendrites was studied in Purkinje cells of the rat cerebellum for 30 days after birth using histochemical, histological, and electron microscopic methods. The Golgi apparatus during differentiation undergoes morphological and positional changes. From the 1st to 7th postnatal day, the Golgi apparatus is found in a supranuclear position, and is connected with the axes of differentiating primary dendrites by beam-like processes. From days 8 to 11 this connection disappears, and most of the Golgi apparatus assumes a lateronuclear and infranuclear position. After the 11th or 12th day, the Golgi apparatus is found in perinuclear and peripheral cytoplasmic positions. The formation of granular endoplasmic reticulum occurs in the vicinity of the perinuclear Golgi apparatus. The differentiation of cell and nuclear forms requires approximately 20 days. The morphological changes of differentiation are discussed in relation to the participation of the Golgi apparatus in the differentiation of dendrites and in the formation of the granular endoplasmic reticulum.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 103 (1970), S. 291-319 
    ISSN: 1432-0878
    Keywords: Primates ; Sense organs ; Olfactory Mucosa ; Histochemistry ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Detailed histochemical studies on the localization of various oxidative and dephosphorylating enzymes and esterases have been made on the olfactory mucosa of the rhesus monkey. The upper part of dendritic process and terminal rods of receptor cells and the upper part of supporting cells show high oxidative enzyme activity. The soma and infranuclear parts of receptor and supporting cells have much lower oxidative enzyme activity. The basal cells and perineural epithelial cells have high hydrolytic enzyme activity, though they show low oxidative enzyme activity. Alkaline phosphatase is localized in the olfactory axons, basal cells and perineural epithelial cells. The receptor and supporting cells contain much less adenosine triphosphatase activity than the basal cells and perineural epithelial cells. The axons of receptor cells pass between the basal cells and come out from the basal cell layer forming the olfactory nerve fasciculi, most of which are surrounded completely by flat squamous cells starting from the base of the basal cell layer. The basal cells are histochemically and cytologically related to the perineural epithelial cells covering the olfactory nerve fasciculi. Basal cells probably cannot give rise to supporting cells and are not young forms of supporting cells. The degenerating receptor cells as well as the olfactory nerve fasciculi which are not completely covered by the flat squamous cells may make the locus minoris resistentiae in the olfactory mucosa. The olfactory axons are rich in most of enzymes we have tested. They seem to be metabolically very active and both cholinergic and adrenergic in nature. The mucosal secretion covering the olfactory epithelium contains oxidative and some hydrolytic enzymes. Bowman's glands show high activity for most enzymes except cholinesterases.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 104 (1970), S. 149-164 
    ISSN: 1432-0878
    Keywords: Acanthocephala ; Parasites ; Epidermis ; Lemniscs ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die synzytiale Epidermis des Darmparasiten Echinorhynchus gadi besteht aus 4 übereinanderliegenden Zonen: apikal befindet sich eine von zahlreichen Poren durchsetzte intrazelluläre elektronendichte Schicht, durch die fingerförmige Einstülpungen des Plasmalemms in die Tiefe ziehen. Diese bilden eine eigene Zone, die durch ihren Gehalt an Enzymen und Pinozytosebläschen gekennzeichnet ist. An eine mittlere Faserschicht, in deren Bereich häufig größere Glykogenfelder lagern, schließt sich basal eine Zone mit zahlreichen Vakuolen und Granula an. Die Lemnisken, die nicht durch eine Plasmamembran von der Epidermis getrennt sind, besitzen auffallend viele Lysomen und Fetteinschlüsse. Die Lemnisken dienen vermutlich der Resorption von Fetten und stellen modifizierte Darmkanäle dar.
    Notes: Summary The syncytial epidermis of the gut-parasite Echinorhynchus gadi consists of four different zones: an apical intracellular electron dense layer, perforated by numerous narrow holes, through which fingerlike invaginations of the surface-plasmalemm penetrate into the cytoplasm, where they constitute an own layer, characterized by specific enzyme activities as well as by pynocytotic vesicles. A central fibre-layer, in which frequently large fields of glycogen occur, is followed by a basal zone with vacuoles and granules. The lemniscs are not separated by a plasmamembrane from the epidermis. They are particularly rich in lysosomes and lipid droplets. They serve the resorption of lipids and are presumably modified intestinal tracts.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 104 (1970), S. 178-192 
    ISSN: 1432-0878
    Keywords: Chiton ; Oogenesis ; Follicle cell ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histochemical studies and electron microscopic investigations on the role of the follicle cells during oogenesis in the chiton Sypharochiton septentriones showed that the main role of the follicle cells was the deposition of a spiny chorion around each oocyte. The chorion was composed of three layers; an inner, acid mucopolysaccharide layer, which was a primary egg membrane secreted by Golgi bodies in the cortical cytoplasm of the oocyte, an intermediate layer of protein and an outer layer of lipid. The intermediate and outer layers were secreted by the follicle cells and were thus secondary egg membranes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...