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  • Biochemistry and Biotechnology  (41)
  • Analytical Chemistry and Spectroscopy
  • Catalysis
  • Wiley-Blackwell  (41)
  • 1960-1964  (41)
  • 1960  (41)
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Keywords
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  • Wiley-Blackwell  (41)
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  • 1960-1964  (41)
Year
  • 1
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Dextran, a polymer of glucose, has been widely used as a blood-plasma volume expander. In whole culture fermentations most dextran-producing organisms elaborate dextrans that have molecular weights of many millions and must be depolymerized and fractionated to obtain a product suitable for clinical use. An exception however is Streptococcus sp. (Strain DS-50) NRRL B-1351. In the work described here, this organism is used to produce clinical-size dextran in high yields, both on a laboratory- and a pilot-plant scale. With this process, fractionation is required but depolymerization is not. Fermentations of media containing 15 per cent sucrose are complete in less than 48 hours with 0·2 per cent inoculum. Yields of native dextran and clinical-size dextran by methanol precipitation are 68 and 43 per cent of the theory, respectively. The structurally heterogeneous dextran formed by NRRL B-1351 has a higher viscosity for a given molecular weight than does NRRL B-512 dextran. The viscosity-molecular weight relationship for the former may be expressed by the equation [η] = 6·51 × 10-4Mw0·554. The high-molecular weight fraction had approximately 75 per cent of 1,6-like linkages, as compared with 87 per cent for the clinical fraction. All fractions had a lower ratio of 1,6 to non-1,6 linkages than did NRRL B-512 dextrans.
    Additional Material: 6 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 447-451 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hot soaking of paddy in the parboiling process does not significantly affect the digestibility of rice compared to ‘raw’ rice. Mechanically dried parboiled rice appears to be slightly more digestible in vitro than sun-dried parboiled rice. It is also significantly superior in thiamine value.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 481-500 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Control of product quality throughout repeated fermentations depends upon maintenance of genetic uniformity from the time of strain selection until the product is harvested. Genetic control, therefore, must be maintained for stock cultures, and during inoculum preparation and growth to produce the product. Theoretical and applied aspects of a procedure that will accomplish this control are discussed. A specific procedure adaptable to situations in which a ‘seed’ is repeatedly increased to produce a larger quantity of a final material is described. The procedure was tested by comparing the product of three bacterial species grown by a standard or usual vs. recommended or experimental procedure comparing product quality and uniformity. Use of the recommended procedure resulted in the high production of a uniform product based on the maintenance of the colony type of Bacillus anthracis, colony colour and type of Serratia marcescens, and uniformity and amount of toxin produced by Clostridium botulinum type A.
    Additional Material: 6 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 31-48 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Production of oligomycin has been studied in fermentations ranging from 100 ml in shaken flasks to 1000 gal in agitated and aerated tanks. The yields ranged from nothing to 3675 μ;g per ml. The main factor favouring the production of oligomycin was a medium giving good growth and supplying an abundance of slowly utilized energy. Media meeting these requirements contained yeast extract and/or molasses for growth and lard oil for energy. Glycerol, glucose or starch as the main source of carbon gave poor results. A pH close to 7 favoured oligomycin production. Considerable organic nitrogen was synthesized and excreted by the mycelium during oligomycin production.Under suitable conditions practically all of the oligomycin remained in the mycelium. Extraction of the mycelium with acetone, concentration of the extract, removal of oily material with a low-boiling hydrocarbon, decolourization and final crystallization from methanol or ethanol were the main steps in the recovery process. From pilot plant and tank fermentations about 4500 g of crystalline oligomycin have been prepared.Three oligomycins, A, B and C were found by paper chromatography in even well-crystallized oligomycin. In different lots the proportions varied from 10-75 per cent A, 4-90 per cent B and 0-36 per cent C. High yields and high percentages of A and C were obtained on the neutral, lard-oil medium. Low yields and high proportions of B were given by the glycerol, low-pH medium.
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  • 5
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A pilot plant was built to evaluate the economic potentialities of producing glycerol from sugar by the bisulphite fermentation process. The design of this small unit was based on information collected from bench-scale studies of the fermentation and recovery processes taking advantage of new separation techniques.A beer containing 5 per cent of glycerol is produced in a continuous fermentation in which the free bisulphite level is automatically controlled. The effluent from the fermentor is centrifuged, and the yeast is returned to the fermentation. The dilute clarified beer is acidified; sulphur dioxide, acetaldehyde, and alcohol are removed; and the stripped solution is neutralized and concentrated until it contains 45 per cent of glycerol. This concentrated glycerol stock is purified by using a combination of ion-exclusion and ion-exchange.Good fermentation control results in a beer that has a low solids-to-glycerol ratio, low organic acid content, and low residual sugar. The method of glycerol purification results in a high glycerol recovery, since no distillation of glycerol is involved. The sulphur dioxide removed from the beer is recycled through the fermentation so that the chief raw materials used in the process are sugar, sulphuric acid, and sodium carbonate. The yields of products based on sugar charged are: glycerol, 25 per cent; alcohol, 17 per cent; and acetaldehyde, 11 per cent.The economic success of the process depends to a large extent upon the price of sugar. Under present conditions it is felt that the process is competitive.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 49-70 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new vibrator, commercially available as the Sonomec Wave-Pulse Generator, is described, in which micro-organisms may be disrupted by shaking with glass beads. The amplitude and frequency of vibration of the shaker may be varied independently by suitable linkages incorporated in the mechanism. A study has been made of the effect of several variables, particularly the height of the liquid column in the vibrating container, on the rate of disruption. A relation is shown to exist between the acoustic heating produced in the container by the passage of sound waves through the liquid and the rate of disruption of baker's yeast. It is considered that at most depths of the liquid column, the action is similar to that of other shakers, but at a certain depth of liquid, additional forces arise which greatly increase the rapidity of disruption. How these forces act is not known. Factors affecting the rate of disruption of baker's yeast, Corynebacterium xerosis and Bacillus megatherium have been studied and a comparison has been made of the fractions obtained by differential centrifugation of the cell dispersion and compared with similar preparations from the Hughes press.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 93-112 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Filtration theory is briefly reviewed with regard to possible application to sterilization by filtration. Of the different devices tested, Seitz asbestos sheets, acid-treated to remove toxic materials, offered most advantages for routine use and scale-up potential. The standard law of Hermans and Bredée best expressed mathematically, the filtration of tissue culture media sterilized by Seitz No. 6 laboratory filters. Data are presented showing the correlation between the performance of the Seitz No. 6 filter, and the Seitz No. 200 plate and frame press (involving a scale-up ratio of up to 400 to 1). An expression: \documentclass{article}\pagestyle{empty}\begin{document}$${{t = 3 \cdot 25 \Delta P^{ - 0 \cdot 627}} \mathord{\left/ {\vphantom {{t = 3 \cdot 25\Delta P^{ - 0 \cdot 627} } {{{(A} \mathord{\left/ {\vphantom {{(A} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}} \right. \kern-\nulldelimiterspace} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}}}} \right. \kern-\nulldelimiterspace} {{{(A} \mathord{\left/ {\vphantom {{(A} {V_f + 0 \cdot 001{\rm }\Delta P - 0 \cdot 036)}}} \right. \kern-\nulldelimiterspace} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}}} $$\end{document} derived from experimental data obtained, expressing time in terms of pressure, surface area, and volume of filtrate, enabled prediction of filtration parameters with accuracy acceptable for engineering purposes for unclarified media, and greatly improved for media which had been initially clarified.The influence of streaming potential is discussed with regard to its influence on the course of a filtration.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 71-80 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ambler's Q/Σ relationship between the throughput of a centrifuge and its mechanical characteristics has been translated from batch data in order to predict operating conditions for continuous centrifugation in virus processing. A Sharples Laboratory Supercentrifuge was converted from batch to continuous operation and a slurry feed device was developed. A correlation between operating conditions for continuous centrifugation and amount of test virus recovered from the feed showed that the Q/Σ values at which high concentrations of the virus were recovered were almost identical to the Q/Σ values for significant virus recovery in batch centrifugation. Titration and gravimetric determinations of the sediments recovered from continuous centrifugation operations indicate that product purity might be influenced by operating conditions.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 121-127 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Equipment has been developed for resuspending or redissolving protein precipitates separated in a continuous-flow centrifuge without first removing them from the centrifuge bowl. All the components can be sterilised by autoclaving. The equipment has been used successfully in selected stages of a process for the preparation of human plasma fractions.
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