ISSN:
0368-1467
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Filtration theory is briefly reviewed with regard to possible application to sterilization by filtration. Of the different devices tested, Seitz asbestos sheets, acid-treated to remove toxic materials, offered most advantages for routine use and scale-up potential. The standard law of Hermans and Bredée best expressed mathematically, the filtration of tissue culture media sterilized by Seitz No. 6 laboratory filters. Data are presented showing the correlation between the performance of the Seitz No. 6 filter, and the Seitz No. 200 plate and frame press (involving a scale-up ratio of up to 400 to 1). An expression: \documentclass{article}\pagestyle{empty}\begin{document}$${{t = 3 \cdot 25 \Delta P^{ - 0 \cdot 627}} \mathord{\left/ {\vphantom {{t = 3 \cdot 25\Delta P^{ - 0 \cdot 627} } {{{(A} \mathord{\left/ {\vphantom {{(A} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}} \right. \kern-\nulldelimiterspace} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}}}} \right. \kern-\nulldelimiterspace} {{{(A} \mathord{\left/ {\vphantom {{(A} {V_f + 0 \cdot 001{\rm }\Delta P - 0 \cdot 036)}}} \right. \kern-\nulldelimiterspace} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}}} $$\end{document} derived from experimental data obtained, expressing time in terms of pressure, surface area, and volume of filtrate, enabled prediction of filtration parameters with accuracy acceptable for engineering purposes for unclarified media, and greatly improved for media which had been initially clarified.The influence of streaming potential is discussed with regard to its influence on the course of a filtration.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jbmte.390020109
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