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  • Articles  (37)
  • cell culture  (37)
  • Springer  (37)
  • American Geophysical Union
  • American Meteorological Society
  • Cell Press
  • Oxford University Press
  • Springer Nature
  • Springer Science + Business Media
  • Wiley
  • 2020-2022
  • 1990-1994  (37)
  • 1960-1964
  • 2022
  • 2021
  • 1991  (37)
  • 1962
  • 1961
  • 1960
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  • Articles  (37)
Source
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  • Springer  (37)
  • American Geophysical Union
  • American Meteorological Society
  • Cell Press
  • Oxford University Press
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  • 2020-2022
  • 1990-1994  (37)
  • 1960-1964
Year
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  • 1
    Electronic Resource
    Electronic Resource
    Toxicity of the mycotoxins fumonisins B1 and B2 and Alternaria alternata f. sp. lycopersici toxin (AAL) in cultured mammalian cells (1991)
    Springer
    Mycopathologia 116 (1991), S. 97-104 
    Details
    ISSN: 1573-0832
    Keywords: Fumonisin B1 ; fumonisin B2 ; AAL toxin ; T-2 toxin ; mycotoxin ; Fusarium moniliforme ; bioassay ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fumonisins B1 and B2 and AAL toxin are a series of structurally related mycotoxins. Fumonisins B1 and B2, produced by Fusarium moniliforme Sheldon induce toxic hepatitis and hepatomas in rats and leukoencephalomalacia in horses. The cancer-promotion assay which has been used to guide their purification is slow and consumes large amounts of sample. We have examined a series of cultured mammalian cell lines in order to develop a more rapid and sensitive bioassay system, which may be useful for examining structure-activity relationships and the mechanism(s) of action of these toxins. Of 9 rat hepatoma cell lines tested, all except the two most de-differentiated lines were sensitive to the three toxins, with a toxic response visible by 48 h. Approximate IC50 values for the most sensitive hepatoma line, H4TG, were 4, 2 and 10 μg/ml for fumonisins B1, B2 and AAL toxin, respectively „in 100 μl cultures. Among 15 cell lines from other sources, only MDCK dog kidney epithelial cells were sensitive (IC50 = 2.5, 2 and 5 μg/ml, respectively). Studies in co-cultures of sensitive and insensitive cell lines and in cultures of a sensitive cell line over a range of cell densities indicated that cytotoxicity of fumonisins B1 and B2 does not involve metabolite activation to a derivative stable enough to diffuse to adjacent cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436371
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  • 2
    Electronic Resource
    Electronic Resource
    Modified culture method for human corneal epithelial cells (1991)
    Springer
    Methods in cell science 13 (1991), S. 45-48 
    Details
    ISSN: 1573-0603
    Keywords: cell culture ; corneal epithelium ; primary explant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An improved procedure for the primary culture of pure human corneal epithelial cells from corneal explants is described. Confluent monolayers of epithelial cells can be consistently produced from small segments of donor corneas regardless of donor age and without feeder layers. Incubating segments on collagen at the air-liquid interface significantly improves the yield of cells per cornea and shortens cell migration time as compared to culturing on plastic. Fibroblast contamination is eliminated by serum-free medium and confirmed by indirect immunofluorescent staining for keratin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388203
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  • 3
    Electronic Resource
    Electronic Resource
    Culture of human renal cortex epithelial cells (1991)
    Springer
    Methods in cell science 13 (1991), S. 143-147 
    Details
    ISSN: 1573-0603
    Keywords: kidney ; renal cortex ; proximal tubule ; enzymatic dissociation ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A procedure is described for the establishment and propagation of epithelial cell rich cultures derived from normal human kidney cortex (NHK-C cells). Cells are harvested from tissue fragments of donor human kidney by progressive enzymatic dissociation. NHK-C cultures are morphologically heterogeneous but exhibit, predominantly, the functional characteristics of cells of the kidney proximal tubule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388118
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of low nitrate and high sugar concentrations on anthocyanin content and composition of grape (Vitis vinifera L.) cell suspension (1991)
    Springer
    Plant cell reports 9 (1991), S. 500-504 
    Details
    ISSN: 1432-203X
    Keywords: anthocyanin ; cell culture ; nitrate ; sucrose ; Vitis vinifera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In pigmented cells of Vitis vinifera suspension cultures, best accumulation of anthocyanins was obtained when nitrate concentration was reduced from 25 mM to 6.25 mM and when sucrose concentration was increased from 88 mM to 132 mM. Under such conditions growth was greatly decreased. However, cell viability was maintained. The increases in anthocyanins in pigmented cells were due largely to increases in peonidin — glucoside. The high sucrose and the low nitrate concentrations can be one of the important culture factors in controlling of anthocyanin production by cell cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232105
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  • 5
    Electronic Resource
    Electronic Resource
    A mechanism for the loss of cytochrome P-450 in primary mouse hepatocytes (1991)
    Springer
    Molecular and cellular biochemistry 108 (1991), S. 151-156 
    Details
    ISSN: 1573-4919
    Keywords: cytochrome P-450 ; mitochondria ; heme ; hepatocytes ; mitochondrial DNA ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This study examined various biochemical parameters such as mitochondria and mitochondrial DNA (mtDNA), total heme and cyto P450 content in fresh hepatocytes and dedifferentiated hepatocytes. These parameters were chosen in order to understand the dramatic decrease in drug metabolism in cultured hepatocytes. The data in this study shows a temporal decrease in cytochrome P450, total heme and also a decrease in mitochondria. Also, the ratio of mtDNA content to mitochondrial density was found to increase as hepatocytes underwent dedifferentiation. Stereological analysis of cell preparations provided a measure of mitochondrial density per cell area and mtDNA content was assessed by the use of a specific radiolabelled probe. This study demonstrates that a loss of the organelle which is partially responsible for synthesis of heme correlates with a decrease in cytochrome P450.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233120
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  • 6
    Electronic Resource
    Electronic Resource
    Adrenergic hormones and control of cardiac myocyte growth (1991)
    Springer
    Molecular and cellular biochemistry 104 (1991), S. 35-43 
    Details
    ISSN: 1573-4919
    Keywords: α1-adrenergic receptors ; β-adrenergic receptors ; cardiac muscle ; cell culture ; gene expression ; protein kinase C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The molecular mechanisms of cardiac myocyte growth are relevant to important problems in cardiovascular disease. A cell culture model has been developed to explore the role of adrenergic hormones in cardiac myocyte growth and gene expression. Activation of a cardiac myocyte α1-adrenergic receptor by catecholamines induces hypertrophic growth of neonatal rat cardiac myocytes and initiates selective increases in contractile protein gene transcription. These effects on growth and gene expression do not depend on contractile activity. The cardiac myocytes contain at least two subtypes of α1-adrenergic receptors and at least three isoforms of protein kinase C (PKC). A distinct α1 receptor subtype may mediate hypertrophy and gene transcription. Different isoforms of PKC are translocated to different intracellular sites on activation, and there is evidence that the β-PKC isoform may be an element in the signal transduction pathway from an α1 receptor at the surface to the cardiac myocyte nucleus. Growth regulation through a β-adrenergic receptor can also be demonstrated in the culture model. The growth response mediated through a β-adrenergic receptor differs in several respects from that transduced through an al adrenergic receptor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229801
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  • 7
    Electronic Resource
    Electronic Resource
    Sequence analysis of a chalcone isomerase cDNA of Phaseolus vulgaris L. (1991)
    Springer
    Plant molecular biology 16 (1991), S. 167-169 
    Details
    ISSN: 1573-5028
    Keywords: Phaseolus vulgaris ; cell culture ; chalcone isomerase ; elicitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017927
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular cloning of a cDNA from Lupinus polyphyllus cell cultures encoding a ribosomal protein (rps16) (1991)
    Springer
    Plant molecular biology 16 (1991), S. 739-740 
    Details
    ISSN: 1573-5028
    Keywords: Lupinus polyphyllus ; cell culture ; cDNA clone ; ribosomal protein ; rps 16
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023439
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  • 9
    Electronic Resource
    Electronic Resource
    Expression and stability of amplified genes encoding 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate-tolerant tobacco cells (1991)
    Springer
    Plant molecular biology 17 (1991), S. 1127-1138 
    Details
    ISSN: 1573-5028
    Keywords: tobacco ; 5-enolpyruvylshikimate-3-phosphate synthase ; cDNA clone ; gene expression ; gene amplification ; glyphosate ; cell culture ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two distinct cDNAs for 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) were obtained from a glyphosate-tolerant tobacco cell line. The cDNAs were 89% identical and the predicted sequences of the mature proteins were greater than 83% identical with EPSPS proteins from other plants. Tobacco EPSPS proteins were more similar to those from tomato and petunia than Arabidopsis. One cDNA clone, EPSPS-1, represented a gene that was amplified in glyphosate-tolerant cells, while the gene for EPSPS-2 was unaltered in these cells. Consequently, EPSPS-1 mRNA was more abundant in tolerant than unselected cells, whereas EPSPS-2 mRNA was at relatively constant levels in these cell lines. Exposure of unselected cells and tobacco leaves to glyphosate produced a transient increase in EPSPS mRNA. However, glyphosate-tolerant cells containing amplified copies of EPSPS genes did not show a similar response following exposure to glyphosate. A significant proportion of the EPSPS gene amplification was maintained when tolerant cells were grown in the absence of glyphosate for eight months. Plants regenerated from these cells also contained amplified EPSPS genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028730
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  • 10
    Electronic Resource
    Electronic Resource
    Studies of serum-free medium for the generation of lymphokine-activated killer cells (1991)
    Springer
    Cytotechnology 6 (1991), S. 39-47 
    Details
    ISSN: 1573-0778
    Keywords: cell culture ; lymphocyte ; lymphokine-activated killer cell ; recombinant interleukin 2 ; serum-free medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We examined a serum-free medium (designated as TYI 101) for the generation of lymphokine-activated killer (LAK) cells from human lymphocytes, regional lymph node lymphocytes (RLNL) and peripheral blood lymphocytes (PBL). TYI 101 medium consisted of, in addition to nutrient mixture, transferrin, insulin, fetuin, sodium selenite, 2-mercaptoethanol, o-phosphorylethanolamine, chick egg yolk and porcine kidney extract. These hormones were effective for supporting RLNL proliferation as assessed by (3H)-thymidine uptake. When human lymphocytes from two different sources were cultivated with recombinant interleukin 2 (rIL-2) in TYI 101 medium, LAK activity was generated. In cultures of PBL from a healthy donor, LAK cells were generated in TYI 101 medium as efficiently as in RPMI 1640 medium supplemented with 10% human AB-type serum (RPMI-AB). In cultures of RLNL from lung cancer patients, LAK activity obtained in TYI 101 medium was about sixty-five percent of that in RPMI-AB. However, the addition of a small amount of AB-type serum improved the generation of LAK activity, LAK cell expansion, and cell viability in TYI 101 medium. We conclude that TYI 101 medium can be used for the generation of LAK cells from human lymph node lymphocytes with supplementation of none or only a reduced amount of human serum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00353701
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