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  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Phytopathology 12 (1974), S. 303-330 
    ISSN: 0066-4286
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 8 (1986), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A Holstein cow was given a single, oral dose of T-2 toxin (200 mg), and blood and urine samples were collected at regular time intervals for 48 h. The animal was dosed once more with the same amount and samples were again collected over another 48 h period. Trace amount (2 ppb) of T-2 toxin was detected in the blood up to 24 h as well HT-2, TC-3, and T-2 tetraol. De-epoxy-T-2-tetraol was detected in the blood and urine up to 48 h. No T-2 could be found in the urine but HT-2, TC-1, TC-3 and T-2-tetraol were detected. In addition, three major metabolites, i.e., iso TC-1, de-epoxy-TC-3 and de-epoxy-T-2 tetraol, were found in the urine. Production of these metabolites was maximum at 14–20 h after administration of the toxin. No substantial accumulation of T-2 toxin derivatives were found in the lung, heart, liver or kidney when the cow was sacrificed at the end of the experiment.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 5 (1983), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zearalenone, a phytoestrogen produced by various species of Fusarium, regulates perithecial formation in F. roseum ‘Graminearum’. The response is concentration dependent, enhancing at low concentrations (1–10 ng) and inhibiting at high concentrations (10–100 μg). The biological activity can be modified by varying the chemical structure of the parent molecule. Zearalenone binds to mammalian estrogen receptor protein competitively with estradiol. Conversely estradiol binds to specific receptor protein from Fusarium mycelium.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 38 (1982), S. 1037-1040 
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of environmental contamination and toxicology 19 (1990), S. 433-436 
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract An isolate ofFusarium oxysporum (MT-6) was obtained from pasture soil in New Zealand in 1987. The isolate was grown on rice and fed to rats in a ratio of 50% and 10% of a complete diet. All rats died within 16 hr and showed mild intestinal and thymic hemorrhage. The major identifiable toxin was moniliformin found at a concentration of 9 mg/g in the rice culture. Purified crystals of moniliformin fed to rats at concentrations of 8, 5 and 3 mg/g of rat diet caused hemorrhaging in the intestines and death of 3 of 3 rats within 16 hr. Dietary concentrations of 2.5 and 2 mg/g of moniliformin killed 2 of 3 rats and concentrations of 1.5 and 1 mg/g killed 1 of 3 rats. No lesions were observed in 3 groups of 3 rats fed a diet containing 0.75, 0.5 or 0.25 mg/g of moniliformin. Intragastric intubation of moniliformin caused hemorrhage of the small intestine and death in 5 of 5 rats at each concentration of 100, 80, 60 and 40 mg/kg body weight and death of 4 of 5 rats at 20 mg/kg. No observable toxic effects were observed in groups of 5 rats each receiving 10, 5 and 2.5 mg of moniliformin/kg body weight.
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  • 6
    ISSN: 1573-0832
    Keywords: mycotoxins ; Fusarium spp. ; trichothecenes ; maize ; deoxynivalenol ; 3-acetyldeoxynivalenol ; 15-acetyldeoxynivalenol ; zearalenone ; moniliformin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Eighty-two cultures of Fusarium species isolated in 1986 from moldy maize in Minnesota were each cultured on rice for 4 weeks and found to produce the following mycotoxins: F. graminearum isolates, deoxynivalenol (DON, 4–225 μg/g), 3-acetyldeoxynivalenol (3-ADON, 2–4μg/g), 15-acetyldeoxynivalenol (15-ADON, 1–35 μg/g) and zearalenone (ZEA, 5–4350 μg/g); F. moniliforme, fusarin C (detectable amounts to 1000 μg/g); F. mòniliforme, F. oxysporum, F. proliferatum and F. subglutinans isolates, moniliformin (15–6775 μg/g); F. moniliforme, F. proliferatum, and F. subglutinans isolates, fusaric acid (detectable amounts). Other mycotoxins screened for in each rice sample and not detected were T-2 toxin, HT-2 toxin, neosolaniol, T-2 tetraol, nivalenol, fusarenon-X, scirpenols, alpha and beta trans-zearalenols, wortmannin, and fusarochromanone. The rat feeding bioassay indicated that other, unidentified toxins may be present.
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  • 7
    ISSN: 1573-0832
    Keywords: Fumonisin B1 ; fumonisin B2 ; AAL toxin ; T-2 toxin ; mycotoxin ; Fusarium moniliforme ; bioassay ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fumonisins B1 and B2 and AAL toxin are a series of structurally related mycotoxins. Fumonisins B1 and B2, produced by Fusarium moniliforme Sheldon induce toxic hepatitis and hepatomas in rats and leukoencephalomalacia in horses. The cancer-promotion assay which has been used to guide their purification is slow and consumes large amounts of sample. We have examined a series of cultured mammalian cell lines in order to develop a more rapid and sensitive bioassay system, which may be useful for examining structure-activity relationships and the mechanism(s) of action of these toxins. Of 9 rat hepatoma cell lines tested, all except the two most de-differentiated lines were sensitive to the three toxins, with a toxic response visible by 48 h. Approximate IC50 values for the most sensitive hepatoma line, H4TG, were 4, 2 and 10 μg/ml for fumonisins B1, B2 and AAL toxin, respectively „in 100 μl cultures. Among 15 cell lines from other sources, only MDCK dog kidney epithelial cells were sensitive (IC50 = 2.5, 2 and 5 μg/ml, respectively). Studies in co-cultures of sensitive and insensitive cell lines and in cultures of a sensitive cell line over a range of cell densities indicated that cytotoxicity of fumonisins B1 and B2 does not involve metabolite activation to a derivative stable enough to diffuse to adjacent cells.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 125 (1994), S. 83-91 
    ISSN: 1573-0832
    Keywords: Colonization ; Computer diskettes ; Fouling ; Fungal isolation ; Magnetic media ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Computer diskettes can be colonized by saprophytic fungi, especially in the humid tropics. Fungi of the generaAlternaria, Aspergillus, Epicoccum, Paecilomyces, Penicillium, andTrichoderma were observed on diskettes from several tropical countries. Common saprophytic fungi from Minnesota colonized clean standard and high density diskettes in growth chambers, indicating that fungal contamination could occur wherever temperature and humidity were adequate.Fusarium species infested diskettes buried in garden soil in Minnesota. Audiotapes, videotapes, and computer magnetic tapes chemically resemble diskettes and also can be colonized by fungi, as can photographic film. The Mylar core of these magnetic media did not support the growth ofPenicillium glabrum, the most aggressive fungus in diskette inoculation studies. However, growth of several fungal species was enhanced when the common plasticizer, lecithin, was added in powdered form to nutrient agar, suggesting that this ingredient of the diskettes may be metabolized by the fungi.
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  • 9
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Twenty samples of unpolished (rough) rice collected in Arkansas and Texas during the 1995 harvesting season from fields exhibiting Fusarium sheath rot disease or panicle blight were previously shown to include 8 samples positive for fumonisin B1(FB1) in the range 2.2–5.2 ppm, and moniliformin (MON), but no beauvericin (BEA), deoxynivalenol, its derivatives or zearalenone were detected. Fifteen cultures of F. proliferatum were established from the 20 rough rice samples. Single spore isolates of each culture were grown on rice and tested for the production of fumonisins (FB1, FB2, FB3, etc.), MON and BEA. All 15 isolates produced FB1, FB2, MON and BEA in culture on rice. No deoxynivalenol, its derivatives orzearalenone were detected. Seven cultures produced FB1 at 〉50ppm (range 80–230 ppm), with therest producing FB1 in the range 14–43 ppm.FB2 was produced in the range 5–47 ppm, and those cultures which produced the most FB1 also produced the most FB2. Of the 15 cultures producing MON, 11 produced it at 〉100 ppm in the range 188–6018 ppm, with the rest producing in the range 7–64 ppm. BEA was produced in the range 109–1350 ppm. Other derivatives of fumonisins, including FA1, FA2 and partially hydrolyzed FB1, as well asseveral unknown metabolites including a compound with MW 414, were identified in culture extracts by continuous flow fast atom bombardment with ion spraymass spectrometry (CF/FAB/MS). Further study is needed to identify the factors that control production of FB1, MON and BEA by F.proliferatu in culture and in field samples.
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  • 10
    ISSN: 1573-0832
    Keywords: Fusarium ; mycotoxins ; sugar beets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Five toxigenic isolates of Fusarium species were tested for the production of zearalenone, moniliformin and trichothecenes (deoxynivalenol, 15-acetyldeoxynivalenol, T-2, HT-2 and neosolaniol) when grown on solid sugar beet slices in the laboratory for thirty days. The isolates were also grown on a solid rice medium for comparison. High zearalenone and trichothecene-producing isolates originally obtained from corn and corn-based feedstuff were compared with isolates obtained from sugar beets. One moniliformin-producing isolate from wheat was included in the study. With the exception of moniliformin, all toxins were produced on both substrates; however, the rice medium yielded the greater concentrations except for HT-2 which was produced on sugar beets in equal or greater concentrations. Zearalenone production on rice reached 729–1943 gmg/g whereas on sugar beet it reached 72–193 gmg/g. The moniliformin-producing isolate grew well on both substrates; however, moniliformin was produced only on the rice substrate. This study demonstrates for the first time that Fusarium species can produce both zearalenone and the trichothecenes on a sugar beet substrate.
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