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  • Immunocytochemistry  (79)
  • Springer  (79)
  • American Chemical Society
  • American Meteorological Society
  • Nature Publishing Group
  • Springer Nature
  • 1985-1989  (78)
  • 1975-1979
  • 1970-1974  (1)
  • 1960-1964
  • 1950-1954
  • 1988  (42)
  • 1985  (36)
  • 1974  (1)
  • 1971
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  • Springer  (79)
  • American Chemical Society
  • American Meteorological Society
  • Nature Publishing Group
  • Springer Nature
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  • 1985-1989  (78)
  • 1975-1979
  • 1970-1974  (1)
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  • 1950-1954
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  • 1
    Digitale Medien
    Digitale Medien
    Occurrence, distribution and nature of neuropeptide Y in the rat pancreas (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1554-1557 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Immunocytochemistry ; neuropeptide Y ; radioimmunoassay ; rat pancreas
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Significant quantities of a newly discovered peptide, neuropeptide Y, were found in the rat pancreas, where they were localized to nerves in the exocrine parenchyma and around arterial and ductal structures. Although unaffected by surgical parasympathectomy, the periarterial and periductal nerves were abolished by chemical sympathectomy, suggesting that NPY is partially costored with sympathetic transmitters in nerve fibers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01964804
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  • 2
    Digitale Medien
    Digitale Medien
    Immunocytochemical demonstration of calmodulin in cells secreting by exocytosis (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1340-1342 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Immunocytochemistry ; calmodulin ; secretory granules
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Calmodulin is a regulator of several calcium-dependent cellular processes. It has been suggested that it plays a role in the mechanism of secretion. Employing an indirect immunoperoxidase technique at the light microscope level, this study demonstrates the presence of calmodulin in several exocytotic cells (mast cells, thyroid follicular cells, neurohypophyseal neurosecretory terminals, pancreaticβ-cells and pancreatic acinus cells) in rat and man. The positive staining reaction for calmodulin was granular and at least in the case of rat mast cells it appeared to be associated with the granule membrane.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01952085
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  • 3
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of two key enzymes of the 2-hydroxyglutarate pathway of glutamate fermentation in Acidaminococcus fermentans (1988)
    Springer
    Archives of microbiology 150 (1988), S. 504-508 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Acidaminococcus fermentans ; Glutamate fermentation ; Electron microscopy ; Immunocytochemistry ; Post-embedding labelling ; Antibody-gold complexes ; Protein A-gold complexes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have investigated the in situ location of glutaconyl-CoA decarboxylase and 2-htdroxyglutaryl-CoA dehydratase in Acidaminococcus fermentans using the antibody-gold and protein A-gold techniques carried out as a post-embedding immunoelectron microscopic procedure. Polyclonal antisera were raised in rabbits against homogeneous fractions of the enzymes. Anaerobically grown cells of A. fermentans of the late exponential growth phase were fixed with 0.2% glutaraldehyde and 0.3% formaldehyde (final concentrations) in the growth medium. Dehydration of the cells was achieved with methanol. The cells were embedded in the low temperature embedding resin Lowicryl K4M. The markers indicative for antigenic sites of the two enzymes unequivocally demonstrate that the sodium pump glutaconyl-CoA decarboxylase is located at the cell periphery being a membrane-bound enzyme as expected whereas 2-hydroxyglutaryl-CoA dehydratase is a soluble cytoplasmic enzyme.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00422295
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  • 4
    Digitale Medien
    Digitale Medien
    Dihydroxyacetone synthase is localized in the peroxisomal matrix of methanol-grown Hansenula polymorpha (1985)
    Springer
    Archives of microbiology 143 (1985), S. 237-243 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Hansenula polymorpha ; Peroxisomes ; Methanol ; Dihydroxyacetone synthase ; Cell fractionation ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The subcellular localization of dihydroxyacetone synthase (DHAS) in the methylotrophic yeast Hansenula polymorpha was studied by various biochemical and immunocytochemical methods. After cell fractionation involving differential and sucrose gradient centrifugation of protoplast homogenates prepared from methanol-grown cells, DHAS cosedimented with the peroxisomal enzymes alcohol oxidase and catalase. Electron microscopy of this fraction showed that it contained mainly intact peroxisomes, whereas SDS-polyacrylamide gel electrophoresis revealed two major protein bands (75 and 78 kDa) which were identified as alcohol oxidase and DHAS, respectively. The localization of DHAS in peroxisomes was further established by immunocytochemistry. After immuno-gold staining carried out on ultrathin sections of methanol-grown H. polymorpha using DHAS-specific antibodies, labelling was confined to the peroxisomal matrix.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00411242
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  • 5
    Digitale Medien
    Digitale Medien
    The major albumin protein from pea (Pisum sativum L.) (1985)
    Springer
    Planta 165 (1985), S. 522-526 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Albumin (localisation) ; Cotyledon ; Pisum (albumin protein) ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The major albumin protein in storage parenchyma tissue of developing peas has been localised at an ultrastructural level by immunocytochemistry. Tissue was fixed in buffered aldehyde and embedded in LR White resin which was polymerised by addition of catalyst. Sections were labelled by the indirect method of absorption of Protein A-gold to specifically bound antibodies. This method gives high levels of specific labelling on sections which retain good ultrastructural preservation and have high contrast after conventional staining. The albumin is located throughout the cytoplasm although no labelling was found associated with the endoplasmic reticulum, Golgi apparatus, vacuoles-protein bodies or other organelles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00398098
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  • 6
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of phaseolin and phytohemagglutinin in the endoplasmic reticulum and Golgi complex of developing bean cotyledons (1985)
    Springer
    Planta 164 (1985), S. 295-302 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Cotyledon ; Golgi complex ; Immunocytochemistry ; Phaseolus (seed proteins) ; Phaseolin ; Phytohemagglutinin ; Protein (seeds)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Development of legume seeds is accompanied by the synthesis of storage proteins and lectins, and the deposition of these proteins in protein-storage vacuoles (protein bodies). We examined the subcellular distribution, in developing seeds of the common bean, Phaseolus vulgaris L., of the major storage protein (phaseolin) and the major lectin (phytohemagglutinin, PHA). The proteins were localized using an indirect immunocytochemical method in which ultrathin frozen sections were immunolabeled with rabbit antibodies specific for either PHA or phaseolin. Bound antibodies were then localized using goat-anti-rabbit immunoglobulin G adsorbed onto 4- to 5-nm colloidal gold particles. The sections were post-fixed with OsO4, dehydrated, and embedded in plastic on the grids. Both PHA and phaseolin exhibited a similar distribution in the storage-parenchyma cells, being found primarily in the developing protein bodies. Endoplasmic reticulum and Golgi complexes (cisternal stacks and associated vesicles) also were specifically labeled for both proteins, whereas the cytosol and other organelles, such as mitochondria, were not. We interpret these observations as supporting the hypothesis that the transport of storage proteins and lectins from their site of synthesis, the rough endoplasmic reticulum, to their site of deposition, the protein bodies, is mediated by the Golgi complex.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00402940
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  • 7
    Digitale Medien
    Digitale Medien
    Urotensin II-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish (1985)
    Springer
    Cell & tissue research 239 (1985), S. 349-354 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Urophysis ; Caudal neurosecretory system ; Urotensin II ; Immunocytochemistry ; Teleosts
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Antiserum generated against synthetic urotensin II of the goby, Gillichthys mirabilis, was used to localize urotensin II in the caudal neurosecretory system in six species of freshwater teleosts; Cyprinus carpio, Carassius auratus, Oreochromis mossambicus, Oreochromis niloticus, Salmo gairdneri and Plecoglossus altivelis, and six species of seawater teleosts: Acanthogobius flavimanus, Pagrus major, Paapristipoma trilineatum, Trachurus japonicus, Seriola dumerili and Seriola quinqueradiata. In the carp, urotensin II-immunoreactive perikarya were classified into three groups according to their size and shape. Small cells were located in the spinal cord dorsal to the urophysis, medium-sized cells immediately anterior to the urophysis, and large cells anterior to the medium-sized cells. In each group, a small number of nonreactive cells was found. Urotensin II-immunoreactive nerve fibers extended toward the urophysis and terminated around the blood vessels. Other species of teleosts showed a similar immunoreaction to that observed in the carp. The immunoreaction of the urophysis was stronger in seawater fish than freshwater fish. Urotensin II-immunoreactive elements could not be detected in the brains of the carp, goldfish and goby.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218014
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  • 8
    Digitale Medien
    Digitale Medien
    Luteinizing hormone —Releasing hormone (LH-RH) pathway of the rat hypothalamus revealed by the unlabeled antibody peroxidase-antiperoxidase method (1974)
    Springer
    Cell & tissue research 153 (1974), S. 211-217 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Luteinizing hormone-releasing hormone ; Hypothalamus ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Utilizing the unlabeled antibody enzyme method, we report the distribution of hypothalamic elements immunoreactive with antibodies to luteinizing hormone-releasing hormone (LH-RH) in the rat. Immunostained elements, resembling neural processes, were distributed along a pathway corresponding to the tuberoinfundibular tract which appeared to terminate near vascular elements in the external layer of the preand post-infundibular median eminence. No cell bodies stained specifically for LH-RH. Similar topographic arrangements were noted (in coronal and sagittal sections) in diestrous females, ovariectomized females and a hypophysectomized male. The same results were obtained with three different preparations of antisera to LH-RH. Our studies agree with those of other investigators using immunohistochemical techniques as well as with localization studies of LH-RH in the hypothalamus using bioassay and radioimmunoassay. Our results suggest that the unlabeled antibody enzyme technique will have unique value for identifying and tracing fiber systems related to specific functions within the hypothalamus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226609
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  • 9
    Digitale Medien
    Digitale Medien
    Immunocytochemical demonstration of retinal S-antigen in the pineal organ of four mammalian species (1985)
    Springer
    Cell & tissue research 239 (1985), S. 81-85 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pineal organ (mammals) ; Retinal S-antigen ; Photoreceptors ; Retina ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary By means of immunocytochemistry retinal S-antigen is selectively demonstrated in retinal photoreceptor cells of the rat and in pinealocytes of the hedgehog, rat, gerbil and cat. Brain areas surrounding the pineal organ are immunonegative. The immunoreactive material is evenly distributed in the perikarya of the cells. Occasionally, inner segments of retinal photoreceptors and processes of pinealocytes are also stained. The outer segments of retinal photoreceptors display a strong immunoreaction. In both pinealocytes and retinal photoreceptors the intensity of the immunoreaction varied considerably among individual cells. The immunocytochemical demonstration of retinal S-antigen in mammalian pinealocytes indicates that these cells still bear characteristics of photoreceptors. This finding is in accord with the concept that mammalian pinealocytes are derived from pineal photoreceptor cells of poikilothermic vertebrates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214906
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  • 10
    Digitale Medien
    Digitale Medien
    Immunocytochemical studies of vasotocin and mesotocin in the hypothalamo-hypophysial system of the chicken (1985)
    Springer
    Cell & tissue research 239 (1985), S. 279-291 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Hypothalamo-hypophysial system ; Vasotocin neurons ; Mesotocin neurons ; Avian hypothalamus ; Immunocytochemistry ; Chicken
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The hypothalamo-hypophysial system of the adult chicken has been studied with a monoclonal antibody that cross-reacts with arginine vasotocin and mesotocin. We have used this antibody on thick (100 μm) sections in conjunction with a peroxidase-conjugated rabbit antimouse antibody that permits the visualization not only of entire perikarya, but also of long portions of their axons and dendrites. Our results confirm older concepts based on classical methods, but the more sensitive immunocytochemical method reveals that the system is more extensive than previously recognized. Immunostained neurons in the chicken are widely scattered in the hypothalamus. In the rostral preoptic region, there are three immunostained neuronal cell groups: 1) a prominent closely packed group that extends along the ventromedial surface, 2) a diffusely distributed lateral group, and 3) an external group that surrounds the lateral aspect of the septomesencephalic tract. Caudally in the preoptic area and in the anterior hypothalamus, the same groups are present; but there are also conspicuous periventricular perikarya. Many of them have processes that project to the lumen of the third ventricle, as well as parallel axons that arch lateroventrally in the hypothalamus. In the midhypothalamic area, the periventricular perikarya and processes are particularly numerous at the level of the palliai commissure. The dorsal periventricular group located at the level of the dorsomedial anterior nucleus of the thalamus are the most caudal perikarya. They extend laterally in a wing-like formation. The immunostained axons from all of these perikarya form a compact hypothalamo-hypophysial tract as they run from the midhypothalamus to the median eminence and converge beneath the third ventricle. Axons branching from this tract innervate the zone externa of the anterior median eminence; another group of axons running in the fibrous layer of the zona interna proceeds to the neural lobe.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218005
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