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  • 1
    Electronic Resource
    Electronic Resource
    A new visualization chamber to study the transient volumetric response of yeast cells submitted to osmotic shifts (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 43 (1994), S. 165-170 
    Details
    ISSN: 0006-3592
    Keywords: visualization chamber ; osmotic pressure ; yeast ; image analysis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A visualization chamber has been developed to analyze potential correlations between osmotic step increase on yeasts and the resultant cell volume decreases. Image analysis was used to characterize the step increases in the center of the chamber and to measure the changes in the cell volume. Step increases of different intensities have been performed on the yeast Saccharomyces cerevisiae. This device has allowed the kinetics of the volumetric evolution of the cells to be observed. The water exit flow rate from the cell was found to occur in the first 10 s following the hypertonic step change. Comparison of the time constants of the chamber and of the cell volume variations allowed to conclude that the time constant of the water transfer across the membrane was short (about 1 s). © 1994 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260430210
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  • 2
    Electronic Resource
    Electronic Resource
    Differences in response of Zymomonas mobilis and Saccharomyces cerevisiae to change in extracellular ethanol concentration (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 43 (1994), S. 155-158 
    Details
    ISSN: 0006-3592
    Keywords: Zymomonas ; yeast ; ethanol ; inhibition ; adaptation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In high cell density batch fermentations, Zymomonas mobilis produced 91 g L-1 ethanol in 90 min but culture viability fell significantly. Similar viability losses in rapid fermentations by yeast have recently been shown to be attributable in part to the high rate of change of the extracellular ethanol concentration. However, in simulated rapid fermentations in which ethanol was pumped continuously to low cell density Z. mobilis suspensions, increases in the rate of change of ethanol concentration in the range 21-83 g L-1 h-1 did not lead to accelerated viability losses. The lag phase of Zymomonas cultures exposed to a 30-g L-1 step change in ethanol concentration was much shorter than that of Saccharomyces cerevisiae, providing evidence that the comparative insensitivity of Zymomonas to high rates of change of ethanol concentration is due to its ability to adapt to changes in ethanol concentration more rapidly than yeast. © 1994 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260430208
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  • 3
    Electronic Resource
    Electronic Resource
    Formation of carotenoids by rhodotorula glutinis in whey ultrafiltrate (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 888-894 
    Details
    ISSN: 0006-3592
    Keywords: Rhodotorula glutinis ; Lactobacillus helveticus ; yeast ; whey ; carotenoids ; carotenogenesis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The growth and carotenoid biosynthesis of the yeast Rhodotorula glutinis was studied by cocultivation with Lactobacillus helveticus in cheese ultrafiltrate containing 3.9% and 7.1% lactose. By growing this mixed culture in a 15-L fermentor MBR AG (Switzerland) at an air flow rate of 0.5 L/L min and agitation at 220 rpm for 6 days, a total yield of carotenoids of 268 μg/g dry cells wasobtained. Carotenoids were formed almost parallel with the cell growth, anda maximum production was reached at an early stationary phase. A high-performance liquid chromatographic system (HPLC) permitting simultaneous determination of major carotenoid pigments was used. The three main pigments (torularhodin, β-carotene, and torulene) were formed in Rhodotorula glutinis, and reached a maximum concentration as follows: 182.0, 43.9, 23.0 μg,g dry cells. © 1994 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260440804
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  • 4
    Electronic Resource
    Electronic Resource
    Chemical and enzymatic extraction of heavy metal binding polymers from isolated cell walls of Saccharomyces cerevisiae (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 297-302 
    Details
    ISSN: 0006-3592
    Keywords: cell walls ; metal binding ; polymers ; yeast ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Isolated cell walls of the yeast Saccharomyces cerevisiae were treated by either chemical (alkali and acid) or enzymatic (protease, mannanase or β-glucuronidase) processes to yield partially purified products. These products were partially characterized by infrared analysis. They were subsequently reacted with heavy metal cation solutions and the quantity of metal accumulated by the cell wall material determined. The Cu2+ ion (0.24, 0.36, 1.12, and 0.60 μmol/mg) was accumulated to a greater extent than either Co2+ (0.13, 0.32, 0.43, and 0.32 μmol/mg) or Cd2+ (0.17, 0.34, 0.39, and 0.32 μmol/mg) by yeast cell walls, glucan, mannan, and chitin, respectively The isolated components each accumulated greater quantities of the cations than the intact cell wall. Removal of the protein component of the yeast cell walls by Pronase caused a 29.5% decrease in metal accumulation by yeast cell walls per mass, indicating the protein is a heavy metal accumulating component. The data indicate that the outer mannan-protein layer of the yeast cell wall is more important than the inner glucan-chitin layer in heavy metal action accumulation. © 1994 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260440307
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  • 5
    Electronic Resource
    Electronic Resource
    Studies of on-line viable yeast biomass with a capacitance biomass monitor (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 43 (1994), S. 337-341 
    Details
    ISSN: 0006-3592
    Keywords: yeast ; on-line ; capacitance ; viable biomass ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A commercially available biomass monitor has been employed in a number of applications. For capacitance monitors, a relationship between capacitance measurement and cell counts or colony forming units has been reported in the literature. However, for use as an online instrument, a more practical correlation with the biomass concentration is needed. In this study, we followed the batch growth of brewer's yeast and a correlation with viable biomass concentration (g DW/L) was demonstrated. This correlation was utilized with the capacitance biomass monitor in a control loop to maintain setpoint biomass levels in a cyclic reactor under perturbations. Not only did the system demonstrate the capability of the biomass monitor to control biomass in such a system, but it also confirmed the correlation reported in our earlier work. © 1994 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260430411
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  • 6
    Electronic Resource
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    Photon emission from chemically perturbed yeast cells (1994)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 9 (1994), S. 59-63 
    Details
    ISSN: 0884-3996
    Keywords: Ultraweak luminescence ; yeast ; photon emission ; stress-induced ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Photon emission (PE) from yeast cells Saccharomyces cerevisiae strain SP-4 in normal conditions and in conditions perturbed by the addition of formaldehyde was investigated using single-photon counting equipment. PE from yeast cells, growing in a standard nutrient medium (YPG) then centrifuged and resuspended in a phosphate buffer (pH = 6.5), was measured in the presence of oxygen or argon. The solution of formaldehyde (2%) was injected into the sample. The intensity of PE increased and reached a maximum, then slowly decreased to a level which was higher than the PE level without the perturbing factor. The kinetics of PE was found to be strongly dependent upon the presence of oxygen. The model of formation and recombination of free radicals was tested. The results indicate that PE can arise during the recombination reactions of free radicals like R⋅ + R⋅, RO⋅ + RO⋅, RO⋅2 + RO⋅2 which are formed in the enzymatic oxidative reactions.
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/bio.1170090204
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  • 7
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    X. Yeast sequencing reports. Sequence and function analysis of a 9·74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1481-1488 
    Details
    ISSN: 0749-503X
    Keywords: Transposon-facilitated DNA sequencing ; SLK1 ; SSP31 ; yeast ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In the framework of the European BIOTECH project for sequencing the Saccharomyces cerevisiae genome, we have determined the nucleotide sequence of the cosmid clone 233 provided by F. Galibert (Rennes Cedex, France). We present here 9743 base pairs of sequence derived from the left arm of chromosome X. This sequence reveals three new open reading frames and includes the published sequence (5′ end and open reading frame) of the gene BCK1/SLK1/SSP31 also identified as ORFAA. Deletion mutants of two earlier unknown open reading frames J0840 and J0904 are viable and the open reading frame J0902 is essential for yeast growth. The sequence has been entered in the EMBL data library under accession number X77923.
    Additional Material: 2 Tab.
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    URL: http://dx.doi.org/10.1002/yea.320101112
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  • 8
    Electronic Resource
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    Extracellular levansucrase of Bacillus subtilis produced in yeast remains in the cell in its precursor form (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 29-38 
    Details
    ISSN: 0749-503X
    Keywords: Heterologous gene expression ; levansucrase precursor ; Bacillus subtilis ; yeast ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Levansucrase, a Bacillus subtilis extracellular enzyme, was not secreted in the culture medium when produced in yeast. The protein accumulated inside the cell in its precursor form which represented 0·3% of total proteins. The absence of any post-translational modifications, such as signal sequence cleavage or addition of N-linked sugars, indicated that this protein did not enter the reticulum secretion pathway.Direct observation of the cells by confocal laser scanning microscopy showed that levansucrase was associated with the cytoplasmic membrane. Subcellular fractionation experiments revealed that levansucrase precursor form is associated with membranes through weak ionic interactions. The purified precursor displayed the same catalytic properties as levansucrase secreted by B. subtilis. Thus yeast could be used as a source of levansucrase precursor allowing its isolation as a pure form on a milligram scale.
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320100104
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  • 9
    Electronic Resource
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    Effect of site-directed mutagenesis of conserved lysine residues upon Pas1 protein function in peroxisome biogenesis (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1613-1620 
    Details
    ISSN: 0749-503X
    Keywords: Peroxisome ; yeast ; Saccharomyces cerevisiae ; site-directed mutagenesis ; AAA-family ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The Pas1 protein (Pas1p) is required for peroxisome biogenesis in Saccharomyces cerevisiae and contains two putative ATP-binding sites, each within a domain which is conserved among members of the recently characterized AAA-family. To elucidate whether both putative ATP-binding sites are essential for Pas1p function, lysine467 of the first and lysine744 of the second putative ATP-binding site were each changed to glutamate by site-directed mutagenesis. While replacement of lysine744 abolished the function of the Pas1 protein in peroxisome biogenesis, replacement of lysine467 had no obvious effect.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320101210
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  • 10
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    Electronic Resource
    Detection of polygalacturonase, pectin-lyase and pectin-esterase activities in a Saccharomyces cerevisiae strain (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1311-1319 
    Details
    ISSN: 0749-503X
    Keywords: Pectinase ; polygalacturonase ; pectinlyase ; pectinesterase ; yeast ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The catalytic capacity of several excreted pectinolytic enzymes obtained from various yeast strains was examined using in vivo and biochemical techniques. Of the 33 yeast strains studied, 30 were isolated from champagne wine during alcoholic fermentation. Only one yeast strain was found to excrete pectinolytic enzymes and was identified as Saccharomyces cerevisiae and designated SCPP. Pulsed-field gel electrophoresis and the polymerase chain reaction technique were used to characterize further this specific strain. Three types of pectinolytic enzymes were found to be excreted by SCPP: polygalacturonase, pectin-lyase and pectin-esterase. These enzymes allow pectin hydrolysis during cell growth.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320101008
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  • 11
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    The in vivo effects of ethidium bromide on mitochondrial and ribosomal DNA in Candida parapsilosis (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1203-1210 
    Details
    ISSN: 0749-503X
    Keywords: Pulsed-field gel electrophoresis ; confocal microscopy ; intercalating dye ; yeast ; rDNA ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The ability of Candida parapsilosis to grow in the presence of high levels of ethidium bromide (EB) has been explored to study the effects of this intercalating dye on DNA in vivo. By employing confocal microscopy we have determined that EB penetrates the cellular membranes and binds rapidly to the nucleolus, whereas mitochondrial DNA becomes stained after a longer exposure to this dye. No detectable staining of the nucleus has been detected under these conditions.Electrophoretic studies of both undigested and restricted DNAs confirm that the nuclear DNA is unaffected by high levels of EB, with the exception of the rDNA-bearing chromosome that undergoes significant structural alterations in the presence of EB. Moreover, the hybridization signal with the rDNA probe is proportionally reduced in samples obtained from cultures grown in the presence of EB, suggesting that the average copy number of rRNA genes in these cultures may be affected.In striking contrast to other fungal species, the linear organelle genome in C. parapsilosis retains its structural and functional integrity in the presence of high concentrations of EB.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320100908
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  • 12
    Electronic Resource
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    Yeast sequencing reports. Sequence of the AFG3 gene encoding a new member of the FtsH/Yme1/Tma subfamily of the AAA-protein family (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1389-1394 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; yeast ; AAA-protein family ; putative ATPase ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A nuclear gene from Saccharomyces cerevisiae was cloned by genetic complementation of a temperature-sensitive respiratory-deficient mutant. DNA sequence analysis reveals that it encodes a protein with homology to Yme1, FtsH and Tma, proteins which belong to the AAA-protein family (ÃPases associated with diverse cellular activities). The members of this family are involved in very different biological processes. Yme1p, a yeast mitochondrial protein, affects the rate of DNA escape from mitochondria to the nucleus and the Escherichia coli FtsH protein is apparently involved in the post-translational processing of PBP3, a protein necessary for septation during cell division. This newly sequenced gene, which we have designated AFG3 for ÃPase family gene 3, encodes a putative mitochondrial protein of 760 amino acid residues that is closely related to FtsH, Tma (protein from Lactococcus lactis) and Yme1p with 58, 55 and 46% identity respectively. The sequence has been deposited in the EMBL data library under Accession Number X76643.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320101016
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  • 13
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    II. Yeast sequencing reports. The sequence of a 32 420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. S47 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; yeast ; chromosome II ; RIF1 ; DPB3 ; MRP-L27 ; SNF5 ; SEC61-homolog ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The sequence of a 32 420 bp segment of Saccharomyces cerevisiae chromosome II has been deduced. The sequence data revealed 19 potential new genes covering 83·5% of the sequence. Four genes had already been cloned and sequenced: part of RIF1, DPB3, MRP-L27 and SNF5. Besides these four genes, 15 open reading frames (ORFs) of at least 100 amino acids encoding potential new genes were identified. Two of these ORFs are overlapping and a third is located within another ORF.The putative gene product of ORF YBR2039 was homologous to the group of uncoupling proteins involved in the mitochondrial energy transfer system. We propose a remapping of the MRP-L27 gene encoding the mitoribosomal protein YmL27 as it previously has been mapped on chromosome X. The ORF YBR2020 has a strong homology with a 31·9% identity in a 473 amino acid region to the yeast gene SEC61, suggesting that YBR2020 is a new gene encoding a protein involved in translocation of proteins in the yeast cell. Six of the potential genes do not exhibit any significant homology to previously sequenced genes as predicted in the Fast A analysis. The sequence has been deposited in the EMBL data library under Accession Number X76053.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320100007
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  • 14
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    I. Yeast sequencing reports. Isolation and characterization of the LEU2 gene of Hansenula polymorpha (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 509-513 
    Details
    ISSN: 0749-503X
    Keywords: LEU2 gene ; gene structure ; evolutionary conservation ; Hansenula polymorpha ; yeast ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A DNA fragment carrying the LEU2 gene of methylotrophic yeast Hansenula polymorpha was isolated by complementation of the leuB mutation of Escherichia coli. The nucleotide sequence of the isolated DNA fragment contains an open reading frame of 363 codons, coding for a protein 80% identical to the LEU2 gene product of Saccharomyces cerevisiae. Further downstream, there is a partial reading frame with no obvious similarity to known proteins. The LEU2 gene of H. polymorpha cannot complement the leu2 mutation of S. cerevisiae. The sequence has been entered in the EMBL data library under the Accession Number U00889.
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    URL: http://dx.doi.org/10.1002/yea.320100410
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  • 15
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    Mating in the heterothallic haploid yeast Clavispora opuntiae, with special reference to mating type imbalances in local populations (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 895-906 
    Details
    ISSN: 0749-503X
    Keywords: Mating ; pheromones ; agglutination ; Clavispora opuntiae ; yeast ; G1 arrest ; nitrogen depletion ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Mating was studied in the haploid, heterothallic yeast Clavispora opuntiae to assess the importance of nutritional, genetic, and other factors that may favour mating and recombination. Local populations of this yeast generally exhibit dramatic inequalities in mating type distributions, suggesting that mating is rare in nature even though most isolates mate freely in the laboratory. The absence of assimilable nitrogen is prerequisite to mating competence, presumably by causing G1 arrest. Maximum mating competence is found in cells entering stationary phase in nitrogen-limited media. Unlike the vast majority of mating yeasts, C. opuntiae does not appear to produce diffusible mating factors (sex pheromones), and mating-competent cells do not undergo sexual agglutination. Pairwise cell contact appears to be the only signal that triggers the sexual process in this case. In order to determine if mating type imbalances in nature are caused by reduced fertility of ‘consanguine’ crosses, meiotic recombination was measured in pairs of strains that varied in their genetic distances as indicated by restriction mapping. That hypothesis was rejected, as recombination efficiency decreased with increasing genetic distance. We conclude that the rarity of mating in local populations is exacerbated by the stringent physical (pairwise cell contact) and nutritional (nitrogen depletion) conditions that will allow mating to proceed. Parallels are drawn with mating patterns observed in Clavispora lusitaniae.
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    URL: http://dx.doi.org/10.1002/yea.320100705
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  • 16
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    II. Yeast sequencing reports. The sequence of a 22·4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine α-adaptin, as well as a new permease and a DNA-binding protein (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1489-1496 
    Details
    ISSN: 0749-503X
    Keywords: α-adaptin ; chromosome II ; COR1 ; DAL4 ; ERD2 ; FUR4 ; proline synthetase ; PRS3 ; ribosomal protein L16 ; Saccharomyces cerevisiae ; yeast ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We report the sequencing of a 22 470 bp DNA fragment from the left arm of Saccharomyces cerevisiae chromosome II. Thirteen open reading frames longer than 300 bp provisionally called YBL0520, YBL0401 to YBL0408 and YBL0410 to YBL0413 have been detected. Five genes were previously sequenced: COR1, encoding a core protein of the mitochondrial coenzyme QH2 cytochrome c reductase complex (Tzagaloff and Crivellone, 1986). PRS3, a proteasome subunit gene (Lee et al., 1992), ERD2, coding for a protein involved in the secretory pathway (Semeza et al., 1990), URA7, which encodes a CTP synthetase (Ozier-Kalogeropoulos et al., 1991) and the gene for the ribosomal protein L16 (Pan et al., 1993). Among the others, YBL0406 shows striking homologies to FUR4 (Jund et al., 1988) and DAL4 (Yoo et al., 1992), the uracyl and allantoin permeases; YBL0520 is a DNA-related protein, possibly involved in gene regulation; YBL0412 shares homologies with the mouse α-adaptins A and C; and YBL0413 is homologous to a protein of Pseudomonas aeruginosa that is likely to be involved in proline biosynthesis. YBL0401, internal to YBL0520, is probably not expressed. The sequence has been deposited in the EMBL data library under accession number X78217
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    URL: http://dx.doi.org/10.1002/yea.320101113
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  • 17
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    The nucleotide sequence and initial characterization of pyruvate decarboxylase from the yeast Hanseniaspora uvarum (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1581-1589 
    Details
    ISSN: 0749-503X
    Keywords: Pyruvate decarboxylase ; Hanseniaspora uvarum ; yeast ; higher alcohols ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have isolated a pyruvate decarboxylase (PDC) gene from the yeast Hanseniaspora uvarum using the Saccharomyces cerevisiae PDC1 gene as a probe. The nucleotide sequence of this gene was determined and compared to PDC genes from yeast and other organisms. The H. uvarum PDC gene is more than 70% identical to the S. cerevisiae PDC isozymes and possesses a putative thiamine diphosphate binding site. The PDC enzyme was purified and partially characterized. The H. uvarum PDC was very similar to other known PDCs; the Km for pyruvate was 0·75 mM, and the enzyme is a homotetramer with subunits of Mr = 57 000. The sequence has been submitted to GenBank under Accession No. U13635.
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    URL: http://dx.doi.org/10.1002/yea.320101207
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  • 18
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    X-XI. Yeast mapping reports. The use of random-breakage mapping to locate the genes APN1 and YUH1 in the Saccharomyces genome, and to determine gene order near the left end of chromosome XI (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 543-554 
    Details
    ISSN: 0749-503X
    Keywords: Random-breakage mapping ; yeast ; APN1 ; YUH1 ; chromosome XI ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have used the previously described technique of random-breakage mapping to locate the two yeast genes APN1 and YUH1. The APN1 locus is located ∼235 kb from the left telomere of chromosome XI, and shows weak (∼53 cM) genetic linkage to ura1. The YUH1 locus is located ∼140 kb from the right telomere of chromosome X, and genetically maps 3·6 cM distal to cdc11. In addition, we show by random-breakage mapping that TRP3 is located ∼45 kb from the left telomere of chromosome XI, whereas FAS1 is ∼110 kb from the same telomere. This supports a gene order on the left distal portion of chromosome XI that agrees with other physical reports but is inverted with respect to Edition 11 of the published genetic map. This report confirms that random-breakage mapping is a rapid and convenient method of locating cloned genes.
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    URL: http://dx.doi.org/10.1002/yea.320100414
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  • 19
    Electronic Resource
    Electronic Resource
    Characterization of lipid particles of the yeast, Saccharomyces cerevisiae (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1421-1428 
    Details
    ISSN: 0749-503X
    Keywords: Lipid particles ; steryl esters ; sterol Δ24-methyltransferase ; apolipoprotein AII ; yeast ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Lipid particles of the yeast, Saccharomyces cerevisiae, were isolated to high purity and their components were analysed. The hydrophobic core of this organelle consists of triacylglycerols and steryl esters, which are almost exclusively located to that compartment. Lipid particles are stabilized by a surface membrane consisting of phospholipids and proteins. Electron microscopy confirmed the purity of the preparations and the proposed structure deduced from biochemical experiments. Major proteins of lipid particles have molecular weights of 72, 52, 43 and 34 kDa, respectively. The 43 kDa protein reacts with an antiserum against human apolipoprotein AII. In lipid particles of the yeast mutant strain S. cerevisiae erg6, which is deficient in sterol Δ24-methyltransferase, this protein is missing thereby identifying the protein and confirming our previous finding (Zinser et al., 1993) that sterol Δ24-methylation is associated with lipid particles. A possible involvement of surface proteins of lipid particles in the interaction with other organelles is discussed with respect to sterol translocation in yeast.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320101105
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