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  • Artikel  (31)
  • Immunohistochemistry  (31)
  • Springer  (31)
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  • Springer  (31)
  • American Meteorological Society
  • American Physical Society (APS)
  • Blackwell Publishing Ltd
  • Cambridge University Press
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  • Medizin  (31)
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  • 1
    Digitale Medien
    Digitale Medien
    Immunohistochemical localization of types I, II, and III collagens in the ossified posterior longitudinal ligament of the human cervical spine (1983)
    Springer
    Calcified tissue international 35 (1983), S. 159-163 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Anticollagen antibodies ; Collagen types ; Immunohistochemistry ; Ossified posterior longitudinal ligament
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary Immunohistochemical localization of types I, II, and III collagen in the ossified posterior longitudinal ligament of the cervical spine was studied using type-specific anticollagen antibodies. In contrast to the normal ligament which contains both types I and III collagens, the ossified matrix, composed of lamellar bone, contains only type I collagen, except for Haversian canals where type III is located in the inner wall. In the transitional region of preossifying ligaments, types III and I are both present. Type II collagen is present in the hyperplastic matrix of the ligament, and cartilage-like cells surrounded by type II collagen are aligned along nonossified ligaments adjacent to the preossifying region. A possible mechanism of matrix transition during the ossification process is given attention.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02405024
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Immunohistochemical study on the morphology of enterochromaffin cells in the human fundic mucosa (1984)
    Springer
    Cell & tissue research 235 (1984), S. 703-705 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Enterochromaffin cells ; Serotonin ; Immunohistochemistry ; Man
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The morphology of enterochromaffin (EC) cells in the human fundic mucosa was investigated at the lightmicroscopic level by means of the unlabeled peroxidase anti-peroxidase method, with the use of a highly specific anti-serotonin serum. EC-cells in the human fundic mucosa were sparsely distributed below the neck portion of the gland, but were found to be rather numerous in its lower half. Immunohistochemistry revealed marked pleomorphic and seemingly polynuclear EC-cells or cells with long, sometimes multipolar cytoplasmic processes. In addition, luminal contacts and contiguity between EC-cells, or interglandular connections were also encountered. The present immunohistochemical procedure permits, for the first time, a clear-cut morphological visualization of the entire population of EC-cells, and reveals the distinctive morphological features of these cells in the human fundic mucosa. These morphological findings imply that EC-cells in the fundic mucosa may be crucial in gastric function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226973
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Catecholamine-containing pancreatic islet cells of the domestic fowl (1984)
    Springer
    Cell & tissue research 237 (1984), S. 239-244 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Islet A cell ; Catecholamine ; Combined microscopy ; Immunohistochemistry ; Fowl
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In an attempt to identify pancreatic islet cells emitting formaldehyde-induced fluorescence (FIF), the pancreatic islets of the domestic fowl were studied by combined fluorescence, ultrastructural, silver-impregnation and immunohistochemical methods in the same section or in consecutive semi-thin and ultra-thin sections. The results indicate that islet cells emitting intense FIF exhibit a strongly argyrophil reaction with the Grimelius' silver method and also immunohistochemical reaction with anti-glucagon serum, but not with anti-5-HT serum. Therefore, the fowl islet A cell, a peptide hormone-producing cell, stores simultaneously catecholamine as biogenic amine. The islet B and D cells did not display any FIF, any argyrophil reaction with the Grimelius' silver method, or any immunoreactivity with anti-glucagon or anti-5-HT sera. The fluorescent but non-argyrophil cells dispersed in the exocrine acinus may well be PP cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217141
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Serotonin and opsin immunoreactivities in the developing pineal organ of the three-spined stickleback, Gasterosteus aculeatus L. (1984)
    Springer
    Cell & tissue research 237 (1984), S. 559-564 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pineal complex (pineal and parapineal organs) ; Development, ontogenetic ; Photoreceptor cells ; Immunohistochemistry ; Serotonin (5-HT) ; Opsin ; Teleost (Gasterosteus aculeatus)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary 5-hydroxytryptamine (5-HT, serotonin)- and opsin-immunoreactive sites were studied in the developing pineal complex of the stickleback, Gasterosteus aculeatus L., by use of light-microscopic indirect immunoperoxidase techniques. 5-HT immunoreactivity first occurs in the pineal organ at the age of 80 h after fertilization and appears to be localized in cells of the photoreceptor type. The outer segments of a few pineal photosensory cells exhibit opsin immunoreactivity at the age of 84 h after fertilization. The number of cells seems to increase until the pineal organ is completely developed. The increase in the number of 5-HT immunoreactive perikarya runs parallel in time to that of the opsinimmunoreactive outer segments. The cells of the parapineal organ show neither opsin nor 5-HT immunoreactivity. The retina of the embryonic stickleback does not display opsin immunoreactivity until after hatching, which takes place about 144 h after fertilization. These results suggest, in the three-spined stickleback, an earlier light-perception capacity for the developing pineal organ than for the retina.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228440
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  • 5
    Digitale Medien
    Digitale Medien
    Ontogeny of endocrine cells in the gut of the insect Periplaneta americana (1984)
    Springer
    Cell & tissue research 238 (1984), S. 421-423 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Gut endocrine cells ; Ontogeny ; Immunohistochemistry ; Pancreatic polypeptide ; Insect
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ontogeny of the endocrine cells of the gut of the cockroach Periplaneta americana was studied by immunohistochemistry. During embryogenesis, the midgut begins to be formed as an outgrowth of the foregut and hindgut invaginations. Gut endocrine cells with pancreatic polypeptide (PP)-like immunoreactivity begin to appear at the anterior and posterior ends of the forming midgut. These cells are restricted to the midgut epithelium, and no mitotic cells with PP-like immunoreactivity are observed. These results strongly suggest that the gut endocrine cells, at least those with PP-like immunoreactivity, are derived from precursor cells they have in common with other epithelial cells of the midgut.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217318
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  • 6
    Digitale Medien
    Digitale Medien
    Distribution of monoamine-containing nerve fibers in the pineal organ of untreated and sympathectomized dogs (1983)
    Springer
    Cell & tissue research 234 (1983), S. 519-531 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pineal organ ; Serotonin ; Immunohistochemistry ; Fluorescence histochemistry ; Sympathectomy ; Dog
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Monoamine (noradrenaline and serotonin)-containing nerve fibers distributed in the pineal organ of the dog were studied by means of histochemistry (formaldehyde-induced fluorescence) and immunohistochemistry (peroxidase-antiperoxidase-PAP method) with the use of a serotonin antiserum. With the fluorescence-histochemical technique a dense network of blue-green fluorescent fibers was demonstrated in the pineal organ. Most of these fibers formed a perivascular plexus and their branches penetrated into the intercellular spaces of the parenchymal cells. Since these fibers completely disappeared ten days after bilateral removal of the superior cervical ganglia, it was confirmed that they are noradrenergic post-ganglionic sympathetic nerve fibers. A few yellow-fluorescent fibers were detected in the proximal part of the organ after ganglionectomy. By the use of the PAP method, intensively immunoreactive parenchymal cells and nerve fibers were demonstrated. The distribution pattern of these fibers was similar to that of the fluorescent sympathetic fibers. After almost all immunopositive fibers had been abolished by sympathectomy, some serotonin-containing fibers remained. The latter could be traced back to a system of serotonin fibers in the epithalamic region. These findings suggest that 1) the sympathetic noradrenergic fibers in the pineal organ of the dog take up serotonin which is released from the pinealocytes, and 2) this organ receives a dual monoamine innervation via peripheral noradrenergic and central serotonergic nerve fibers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218648
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Mitotic activity of prolactin cells in the pituitary glands of male and female rats of different ages (1984)
    Springer
    Cell & tissue research 235 (1984), S. 497-502 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Prolactin cell ; Mitosis ; Sex difference ; Aging ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The anterior pituitary of colchicine-pretreated male and female rats from 20 days to 12 months of age was stained immunohistochemically with anti-rat prolactin serum. Immunoreactive mitotic cells were identified in all groups of rats. In adult female rats the mitotic index of prolactin cells was higher at oestrus than at other stages of the oestrus cycle and than that in male rats of comparable ages. If adult female rats were ovariectomized on the second day of dioestrus or on the day of proestrus, the mitotic indices at presumptive oestrus were less than those in sham-operated controls at oestrus. Estrogen administration to ovariectomized rats significantly elevated the mitotic index of prolactin cells at 48 h after the treatment. The mitotic indices of prolactin cells in female rats reached a peak at 60 days of age, and then decreased with age. In male rats the mitotic indices showed a steady decrease from the value at 20 days of age. A sex difference in the mitotic indices of prolactin cells was noted from 60 days to 12 months of age. The present results clearly demonstrate that differentiated prolactin cells can undergo mitosis and that a sex difference in the mitotic activity of prolactin cells is present during adult life.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226945
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Immunohistochemical demonstration of serotonin-containing nerve fibers in the hypothalamus of the monkey, Macaca fuscata (1984)
    Springer
    Cell & tissue research 236 (1984), S. 495-503 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Hypothalamus ; Serotonin ; Neuroendocrine regulation ; Monkey (Macaca fuscata) ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distributional pattern of serotonin-containing nerve fibers in the hypothalamus of the monkey (Macaca fuscata) was analyzed with the use of the peroxidaseantiperoxidase method in conjunction with a highly sensitive and specific anti-serotonin serum. The highest concentrations of serotonin-immunoreactive varicose fibers were found in the nucleus praeopticus medialis, nucleus ventromedialis hypothalami, and the complex of mammillary nuclei (nucleus praemamillaris, supramamillaris, mamillaris medialis et lateralis). However, the nucleus suprachiasmaticus, where numerous serotoninergic fibers have been reported to occur in the rat, appeared to be almost devoid of these fibers. The infundibular stalk, and the intermediate and posterior lobes of the pituitary contained considerable numbers of immunoreactive fibers. The present study provides a morphological basis for possible clarification of the influence of serotoninergic projections on various neuroendocrine mechanisms in primates. Furthermore, an attempt was made to clarify the differences and similarities concerning the distributional patterns of serotoninergic nerve fibers within the monkey hypothalamus in contrast to the rat hypothalamus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217216
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  • 9
    Digitale Medien
    Digitale Medien
    Sustentacular cells in the fetal human adrenal medulla are immunoreactive with antibodies to brain S-100 protein (1984)
    Springer
    Cell & tissue research 236 (1984), S. 733-735 
    Details
    ISSN: 1432-0878
    Schlagwort(e): S-100 protein ; Adrenal medulla ; Sustentacular cells ; Human fetus ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Adrenal glands of human fetuses were investigated by means of an immunohistochemical method with the use of an anti-S-100 serum. S-100-immunoreactivity was recognized in sustentacular cells located among the chromaffin cells. A characteristic circular arrangement of the immunostained cells was found in the central region of the adrenal glands. It surrounded aggregations of non-argyrophilic, small, round cells, which were identified as the remaining sympathoblasts (primitive sympathetic cells).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217246
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Catecholamine-synthesizing enzymes in paraganglia of aged Fischer-344 rats (1984)
    Springer
    Cell & tissue research 238 (1984), S. 217-220 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunohistochemistry ; Paraganglia ; Aging ; Catecholamines ; Catecholamine-synthesizing enzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The catecholamine-synthesizing enzymes, tyrosine hydroxylase, dopamine-β-hydroxylase and phenylethanolamine-N-methyltransferase were examined by immunohistochemistry in hypertrophied paraganglia of aged male Fischer-344 rats. All paraganglionic cells reacted with antibodies against tyrosine hydroxylase. Dopamine β-hydroxylase was identified in most paraganglionic cells, indicating that they synthesized norepinephrine. A variable number of paraganglia were positive for phenylethanolamine-N-methyltransferase, which suggested that they synthesized epinephrine. The formaldehyde-induced fluorescence method demonstrated greenish-yellow fluorescence or yellowish-brown fluorescence. The intensity of the fluorescence was in the same range as in adrenal medullary cells. The observations indicate that paraganglia are capable of synthesizing epinephrine.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217291
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