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  • Artikel  (29)
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  • American Association for the Advancement of Science (AAAS)  (29)
  • American Geophysical Union
  • American Meteorological Society
  • International Union of Crystallography (IUCr)
  • Springer Nature
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  • 1
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    Neuroscience. A new look at how neurons compute (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2000-10-21
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Barinaga, M -- New York, N.Y. -- Science. 2000 Sep 29;289(5488):2256-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11041785" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Chloride Channels/metabolism ; Chlorides/metabolism ; Culture Techniques ; Dendrites/physiology ; Motion Perception/*physiology ; Neural Inhibition ; Patch-Clamp Techniques ; Rabbits ; Retinal Ganglion Cells/*physiology ; Signal Transduction ; Sodium Channels/metabolism
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
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    Translating biomolecular recognition into nanomechanics (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2000-04-15
    Beschreibung: We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fritz, J -- Baller, M K -- Lang, H P -- Rothuizen, H -- Vettiger, P -- Meyer, E -- Guntherodt, H -- Gerber, C -- Gimzewski, J K -- New York, N.Y. -- Science. 2000 Apr 14;288(5464):316-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉IBM Research, Zurich Research Laboratory, Saumerstrasse 4, CH-8803 Ruschlikon, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10764640" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antibody Specificity ; Base Pair Mismatch ; Base Pairing ; Chemistry, Physical ; Goats ; Gold/*chemistry ; Hydrogen Bonding ; Immunoglobulin Constant Regions/*chemistry ; Ligands ; *Nucleic Acid Hybridization ; Oligodeoxyribonucleotides/*chemistry ; Physicochemical Phenomena ; Rabbits ; Silicon/*chemistry ; Staphylococcal Protein A/*chemistry ; Static Electricity ; Stress, Mechanical ; Thionucleotides/chemistry
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 3
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    Dual signaling regulated by calcyon, a D1 dopamine receptor interacting protein (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2000-03-04
    Beschreibung: The synergistic response of cells to the stimulation of multiple receptors has been ascribed to receptor cross talk; however, the specific molecules that mediate the resultant signal amplification have not been defined. Here a 24-kilodalton single transmembrane protein, designated calcyon, we functionally characterize that interacts with the D1 dopamine receptor. Calcyon localizes to dendritic spines of D1 receptor-expressing pyramidal cells in prefrontal cortex. These studies delineate a mechanism of Gq- and Gs-coupled heterotrimeric GTP-binding protein-coupled receptor cross talk by which D1 receptors can shift effector coupling to stimulate robust intracellular calcium (Ca2+i) release as a result of interaction with calcyon. The role of calcyon in potentiating Ca2+-dependent signaling should provide insight into the D1 receptor-modulated cognitive functions of prefrontal cortex.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lezcano, N -- Mrzljak, L -- Eubanks, S -- Levenson, R -- Goldman-Rakic, P -- Bergson, C -- MH56608/MH/NIMH NIH HHS/ -- P50 MH068789/MH/NIMH NIH HHS/ -- P50 MH44866/MH/NIMH NIH HHS/ -- R01 MH063271/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 2000 Mar 3;287(5458):1660-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA 30912-2300, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10698743" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Benzazepines/pharmacology ; Brain/cytology/metabolism ; Calcium/metabolism ; Calcium Signaling ; Cell Line ; Cyclic AMP/metabolism ; Dendrites/chemistry/metabolism ; Dopamine Agonists/pharmacology ; Female ; Heterotrimeric GTP-Binding Proteins/metabolism ; Humans ; Macaca mulatta ; Membrane Proteins/analysis/chemistry/genetics/*metabolism ; Molecular Sequence Data ; Prefrontal Cortex/cytology/*metabolism ; Pyramidal Cells/chemistry/*metabolism ; Rabbits ; *Receptor Cross-Talk ; Receptors, Dopamine D1/analysis/*metabolism ; Receptors, Neurotransmitter/metabolism ; Recombinant Fusion Proteins/metabolism ; Signal Transduction ; Two-Hybrid System Techniques
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 4
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    HSV latency-associated transcript and neuronal apoptosis (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2000-09-23
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thompson, R L -- Sawtell, N M -- New York, N.Y. -- Science. 2000 Sep 8;289(5485):1651.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Genetics, University of Cincinnati Medical Center, Cincinnati, OH 45267-0524, USA. Richard.Thompson@UC.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11001720" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antibody Specificity ; *Apoptosis ; Blotting, Western ; Cell Nucleus/enzymology ; Cytoplasm/enzymology ; DNA Fragmentation ; Genes, Viral ; Herpesvirus 1, Human/genetics/*physiology ; Humans ; Immunohistochemistry ; In Situ Nick-End Labeling ; Mice ; Neurons/*cytology/*virology ; Poly(ADP-ribose) Polymerases/analysis/immunology ; Rabbits ; Virus Latency/*genetics
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
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    Dendritic computation of direction selectivity by retinal ganglion cells (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2000-09-29
    Beschreibung: Direction-selective ganglion cells (DSGCs) in the retina respond strongly when stimulated by image motion in a preferred direction but are only weakly excited by image motion in the opposite null direction. Such coding represents an early manifestation of complex information processing in the visual system, but the cellular locus and the synaptic mechanisms have yet to be elucidated. We recorded the synaptic activity of DSGCs using strategies to observe the asymmetric inhibitory inputs that underlie the generation of direction selectivity. The critical nonlinear interactions between the excitatory and inhibitory inputs took place postsynaptically within the dendrites of the DSGCs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Taylor, W R -- He, S -- Levick, W R -- Vaney, D I -- New York, N.Y. -- Science. 2000 Sep 29;289(5488):2347-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉John Curtin School of Medical Research, Centre for Visual Sciences, Department of Psychology, Australian National University, Canberra, ACT 2601, Australia. Rowland.Taylor@anu.edu.au〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11009420" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Action Potentials ; Animals ; Chloride Channels/metabolism ; Chlorides/metabolism ; Culture Techniques ; Dendrites/*physiology ; Excitatory Postsynaptic Potentials ; Interneurons/physiology ; Motion Perception/*physiology ; Neural Inhibition ; Patch-Clamp Techniques ; Rabbits ; Retinal Ganglion Cells/*physiology ; Sodium Channels/metabolism ; Synapses/physiology ; Synaptic Transmission ; gamma-Aminobutyric Acid/physiology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 6
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    Virus-induced neuronal apoptosis blocked by the herpes simplex virus latency-associated transcript (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2000-02-26
    Beschreibung: Latent infections with periodic reactivation are a common outcome after acute infection with many viruses. The latency-associated transcript (LAT) gene is required for wild-type reactivation of herpes simplex virus (HSV). However, the underlying mechanisms remain unclear. In rabbit trigeminal ganglia, extensive apoptosis occurred with LAT(-) virus but not with LAT(+) viruses. In addition, a plasmid expressing LAT blocked apoptosis in cultured cells. Thus, LAT promotes neuronal survival after HSV-1 infection by reducing apoptosis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Perng, G C -- Jones, C -- Ciacci-Zanella, J -- Stone, M -- Henderson, G -- Yukht, A -- Slanina, S M -- Hofman, F M -- Ghiasi, H -- Nesburn, A B -- Wechsler, S L -- EY07566/EY/NEI NIH HHS/ -- EY11629/EY/NEI NIH HHS/ -- New York, N.Y. -- Science. 2000 Feb 25;287(5457):1500-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Ophthalmology Research Laboratories, Cedars-Sinai Medical Center Burns & Allen Research Institute, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10688801" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Apoptosis ; Cell Line ; Genes, Viral ; Herpesvirus 1, Human/genetics/*physiology ; Immunohistochemistry ; In Situ Nick-End Labeling ; Keratitis, Herpetic/*pathology/*virology ; Mutation ; Neurons/*pathology/virology ; Poly(ADP-ribose) Polymerases/immunology/metabolism ; Rabbits ; Transcription, Genetic ; Trigeminal Ganglion/pathology/virology ; Virus Activation ; Virus Latency/*genetics
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 7
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    Bioactive cardiac substances: potent vasorelaxant activity in mammalian atria (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1983-07-01
    Beschreibung: Mammalian atrial extracts possess natriuretic and diuretic activity. In experiments reported here it was found that atrial, but not ventricular, extract also causes relaxation of isolated vascular and nonvascular smooth muscle preparations. The smooth muscle relaxant activity of atrial extract was heat-stable and concentration-dependent and could be destroyed with protease. Rabbit aortic and chick rectum strips were used for the detection of atrial biological activity. The atrial activity was separated by column chromatography into two peaks having apparent molecular weights of 20,000 to 30,000 and less than 10,000. The atrial substance that copurified with the smooth muscle relaxant activity in both peaks caused natriuresis when injected into conscious rats. It appears that atria possess at least two peptides that elicit smooth muscle relaxation and natriuresis, suggesting an endogenous system of fluid volume regulation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Currie, M G -- Geller, D M -- Cole, B R -- Boylan, J G -- YuSheng, W -- Holmberg, S W -- Needleman, P -- New York, N.Y. -- Science. 1983 Jul 1;221(4605):71-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6857267" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Atrial Function ; Chickens ; Chromatography, Gel ; Dogs ; Dose-Response Relationship, Drug ; Humans ; Molecular Weight ; Muscle, Smooth/drug effects ; Muscle, Smooth, Vascular/*drug effects ; Natriuresis/drug effects ; Rabbits ; Rats ; Swine ; Vasodilation/drug effects
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 8
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    Protein phosphatases: properties and role in cellular regulation (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1983-07-22
    Beschreibung: Protein phosphorylation is a principal regulatory mechanism in the control of almost all cellular processes. The nature of the protein phosphatases that participate in these reactions has been a subject of controversy. Four enzymes, termed protein phosphatases 1, 2A, 2B, and 2C, account for virtually all of the phosphatase activity toward phosphoproteins involved in controlling glycogen metabolism, glycolysis, gluconeogenesis, fatty acid synthesis, cholesterol synthesis, and protein synthesis. The properties, physiological roles, and mechanisms for regulating the four protein phosphatases are reviewed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ingebritsen, T S -- Cohen, P -- New York, N.Y. -- Science. 1983 Jul 22;221(4608):331-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6306765" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Calcium/physiology ; Cyclic AMP/metabolism ; Glycogen/metabolism ; Liver/enzymology ; Muscles/enzymology ; Phosphoprotein Phosphatases/classification/*physiology ; Phosphoproteins/metabolism ; Phosphorylase Phosphatase/metabolism ; Phosphorylation ; Protein Biosynthesis ; Protein Kinases/physiology ; Rabbits ; Rats
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 9
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    Oxygen tension regulates the expression of angiogenesis factor by macrophages (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1983-09-23
    Beschreibung: When cultured in a hypoxic environment similar to that found in the center of a wound, macrophages secreted active angiogenesis factor into the medium. Under conditions similar to those of well-oxygenated tissue, macrophages did not secrete active angiogenesis factor. Macrophages that secreted the factor at hypoxic conditions stopped secreting it when returned to room air. Thus the control of angiogenesis in wound healing may be the result of macrophages responding to tissue oxygen tension without the necessity of interacting with other cell types or biochemical signals.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Knighton, D R -- Hunt, T K -- Scheuenstuhl, H -- Halliday, B J -- Werb, Z -- Banda, M J -- GM27345/GM/NIGMS NIH HHS/ -- HL26323/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1983 Sep 23;221(4617):1283-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6612342" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Angiogenesis Inducing Agents/*biosynthesis ; Animals ; Anoxia/physiopathology ; Cells, Cultured ; Cornea ; Growth Substances/*biosynthesis ; Macrophages/*physiology ; Models, Biological ; Oxygen/*physiology ; Rabbits ; *Wound Healing
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 10
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    Cholesterol--heart disease link illuminated. New findings explain how blood cholesterol levels are controlled and how to lower them substantially in persons at high risk of heart disease (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1983-09-16
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kolata, G -- New York, N.Y. -- Science. 1983 Sep 16;221(4616):1164-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6310747" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Anticholesteremic Agents/therapeutic use ; Cholesterol/*blood ; Coronary Disease/drug therapy/*etiology ; Humans ; Lovastatin ; Naphthalenes/therapeutic use ; Rabbits ; Receptors, Cell Surface/physiology ; Receptors, LDL
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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