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  • wheat
  • Springer  (807)
  • Elsevier  (167)
  • Frontiers Media SA  (2)
  • American Institute of Physics (AIP)
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  • 101
    Electronic Resource
    Electronic Resource
    Comparison of two crossing and four selection schemes for yield, yield traits, and slow rusting resistance to leaf rust in wheat (1998)
    Springer
    Euphytica 100 (1998), S. 35-43 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita ; leaf rust ; breeding ; resistance ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The most important breeding objectives in crop improvement are improving grain yield, grain quality, and resistances to various biotic and abiotic stresses. The objectives of our study were to compare two crossing and four selection schemes for grain yield, yield traits, and slow rusting resistance to leaf rust (Puccinia recondita) based on additive genes in wheat (Triticum aestivum), and to identify the most efficient crossing and selection methodologies in terms of genetic gains and cost efficiency. Segregating populations were derived from 18 simple crosses and the same number of top (three-way) crosses. Half of the crosses were derived from Yecora 70 and the other half from Veery #10 as the common leaf rust susceptible parents. The four selection schemes were: pedigree, modified bulk (F2 and F1-top as pedigree, selected lines in F3, F4, F2-top, F3-top as bulk; and pedigree in F5 and F4-top populations), selected bulk (selected plants in F2, F3, F4, F1-top, F2-top and F3-top as bulk; and pedigree in F5 and F4-top populations), and nonselected bulk (bulk in F2, F3, F4, F1-top, F2-top and F3-top; and pedigree in F5 and F4-top populations). A total of 320 progeny lines, parents and checks were tested for grain yield, other agronomic traits and leaf rust resistance during the 1992/93 and 1993/94 seasons in Ciudad Obregon (Sonora State, Mexico) which represents a typical high yielding irrigated site. The influence of the type of cross and the selection scheme on the mean grain yield and other traits of the progenies was minimal. The selection of parents was the most important feature in imparting yield potential and other favourable agronomic traits. Moreover, the highest yielding lines were distributed equally. Progeny lines derived from Veery #10 crosses had significantly higher mean grain yield compared to those derived from the Yecora 70 crosses. Furthermore, a large proportion of the highest yielding lines also originated from Veery #10 crosses. Mean leaf rust severity of the top cross progenies was lower than that of the simple cross progenies possibly because two parents contributed resistance to top cross progenies. Mean leaf rust severity of the nonselected bulk derivatives was twice that of lines derived from the other three schemes. Selected bulk appears to be the most attractive selection scheme in terms of genetic gains and cost efficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018391519757
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  • 102
    Electronic Resource
    Electronic Resource
    Breeding for breadmaking quality using old Hungarian wheat varieties (1998)
    Springer
    Euphytica 100 (1998), S. 179-182 
    Details
    ISSN: 1573-5060
    Keywords: wheat ; breadmaking quality ; HMW glutenin subunits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Due to their broad population diversity, old wheat varieties or landraces play an important role in increasing the genetic variability of agronomic traits. On these grounds, an analysis was made of the high molecular weight (HMW) glutenin subunit composition of the old Hungarian wheat variety Bánkúti 1201. It was found that several genotypes with differing breadmaking qualities can be distinguished for this character. When using old varieties in breeding, it is possible to broaden the genetic background of characters responsible for breadmaking quality by separating the populations. A more detailed analysis of the protein composition of germplasm created in this way will be required to obtain a better understanding of this complex character for its conscious introduction into breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018373805161
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  • 103
    Electronic Resource
    Electronic Resource
    Grain yield and quality: does there have to be a trade-off? (1998)
    Springer
    Euphytica 100 (1998), S. 183-188 
    Details
    ISSN: 1573-5060
    Keywords: agronomy ; grain quality ; protein ; sievings ; wheat ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of agronomic practices and cultivars on grain yield, grain protein and small grain sievings was examined in field experiments over four years in the winter rainfall wheatbelt of Western Australia. Rotation with legume crops and pastures was the main factor responsible for increasing grain protein percent. Grain proteins were increased by 4-5% for crops grown in good legume pasture rotations compared to continuous wheat rotations, but only by 1-2% by factors such as delayed sowing time, applied nitrogen, cultivar or grass weed control. In legume based rotations, wheat crops sown at their highest yielding times produced proteins in the appropriate ranges for premium paying grades. Applying N fertilisers up to the optimum rates for yield did not result in proteins below the levels required for premium paying grades, except for hard wheats at 〉11.5% grain protein. Legume rotations and appropriate soil types were required for hard wheats to exceed 11.5% at economic N rates. The yield penalty often associated with high quality cultivars has been reduced or eliminated in the modern cultivars used in the experiments. Some longer season cultivars only produced grain proteins 〉10% if sown after their optimum time for yield, but sowing at optimum time reduced the probability of producing small grain sievings. Some cultivars were more susceptible than others to producing excessive sievings, especially those with inherently smaller than average seed size. Seed rates up to the optimum for grain yield did not result in excessive small grain sievings except where the site was highly fertile, where the crop was sown too late for optimum yield or where too much N fertiliser was used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018326006069
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  • 104
    Electronic Resource
    Electronic Resource
    Impact of plant genetic resources on wheat breeding (1998)
    Springer
    Euphytica 100 (1998), S. 295-303 
    Details
    ISSN: 1573-5060
    Keywords: breeding ; diversity ; donor ; genetics ; Rht-gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This article describes the impact of plant genetic resources on wheat breeding. It defines the important contribution of N.I. Vavilov Institute (St. Petersburg, Russia) to broadening the genetic diversity of new wheat cultivars. Special attention is given to conducting a comprehensive evaluation of intraspecific variability for valuable characters, including: 1) formation of special subcollections, consisting of accessions with useful characters and accessions representing intraspecies diversity for a given character; 2) revealing genetic differences among phenotypically superior accessions; 3) determining a genetic system of intraspecific variability for a given character; 4) formation of a genetic collection; 5) revealing and developing the donors of useful characters. An example using plant height shows that the proposed research approach adequately determines the genetic potential of species and reveals the most effective genes for practical uses. It is assumed that the main sources of genes for breeding Triticum aestivum L. in order of importance will remain: 1) intraspecific diversity of T. aestivum itself; 2) other Triticum species; 3) other genera of the Triticeae Dum. tribe (particularly Aegilops L.); 4) more remote genera of the Poaceae Barnh. botanical family. It is stressed that existing diversity of T. aestivum has been poorly investigated genetically and only partially used by breeders. Properly evaluated, it can provide multiple solutions for traditional and new problems of wheat improvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018380901468
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  • 105
    Electronic Resource
    Electronic Resource
    Crossability of tetraploid wheat landraces native to Sichuan, Shaanxi, Gansu and Xinjiang provinces, China with rye (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 57-62 
    Details
    ISSN: 1573-5109
    Keywords: crossability ; landrace ; rye ; Secale cereale ; Triticum turgidum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The crossability percentages of 131 landraces of wheat (Triticum turgidum L.) native to Sichuan, Shaanxi, Gansu and Xinjiang provinces of China with rye (Secale cereale L.) were tested. 45 landraces showed crossability percentages higher than 5% with 5 landraces expressing crossability percentages over 60%. The geographical distribution of crossability of these tetraploid wheat landraces was reported. Some utilization of high crossability resources was also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008669429068
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  • 106
    Electronic Resource
    Electronic Resource
    Evaluation of shape Aegilops tauschii Coss for resistance to physiological strains CYR30 and CYR31 of wheat stripe rust in China (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 395-398 
    Details
    ISSN: 1573-5109
    Keywords: Aegilops tauschii ; stripe rust ; rust resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Stripe rust is one of the most serious diseases of wheat in China. Two new stripe rust physiological strains CYR30 (Inter. name: 175 E 191) and CYR31 (Inter. name: 239 E 175) have become the dominant and epidemic physiological strains since 1994 in China. Resistance to these strains of 48 Ae. tauschii accessions was evaluated at the seedling and adult stages. Out of 48, 28 accessions displayed seedling and adult resistance. All of the Chinese Ae. tauschii accessions were susceptible at seedling and adult stages. Seedling resistance was highly related to adult resistance in Ae. tauschii. The new genetic resources of resistance to CYR30 and CYR31 could be incorporated into commercial wheat varieties for wheat resistance breeding by direct hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008651901159
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  • 107
    Electronic Resource
    Electronic Resource
    Farmers' valuation and conservation of crop genetic resources (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 139-150 
    Details
    ISSN: 1573-5109
    Keywords: crop genetic resources ; Turkey ; farm ; valuation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper focuses on the value of landraces (traditional and local crop varieties) to farmers in centers of agricultural diversity. Additional information on the factors contributing to the private value which farmers assign to landraces may help to identify a strategy for ensuring the conservation of the crop genetic resources (CGRs) which are embodied in landraces while at the same time minimizing the costs. Economic and ethnobotanical approaches for examining the value of landraces complement one another. A formal economic approach establishes a framework for quantitative analysis while ethnobotanical methods provide qualitative data for assessing the likelihood that particular farmers or farm sectors will maintain landraces. Our research synthesizes the two approaches in order to examine farmer selection of local wheat landraces in relation to that of modern varieties in three provinces in western Turkey. Multiple farmer concerns (e.g. yield, risk, quality), environmental heterogeneity, and missing markets contribute to the persistence of landraces. Household characteristics informing variety choice will also affect the household's perceptions of the importance and value of landraces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008650819946
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  • 108
    Electronic Resource
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    The potential of microsatellites for high throughput genetic diversity assessment in wheat and barley (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 415-421 
    Details
    ISSN: 1573-5109
    Keywords: barley ; diversity ; microsatellites ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Microsatellite (SSR) profiles from 65 wheats and 135 barleys have been generated, involving 14 and 22 loci, respectively. The wheat and barley varieties were chosen to represent the bulk of the area sown to these crops in the UK over the past 70 years. The profiling identified genotypic mixtures in some seed samples. Null alleles were common in wheat, but rare in barley. We describe attempts to increase the efficiency of data acquisition. High resolution agarose gel electrophoresis was unable to satisfactorily resolve 1–2 repeat unit differences in the common size range for SSR loci, and was therefore unsuitable for mass screening of allelic variants. Multiplex PCR was very dependent on the choice of primer combinations and seldom produced amplifications as consistently as when primer pairs were used individually. Background (non-specific) amplification was common to many primer pairs, and this hindered the use of both multiplex PCR and multiple sample loading. Sequential sample loading was the most effective strategy, although this was the least time-efficient of the measures used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008612917967
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  • 109
    Electronic Resource
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    Efficiency of P utilization by pigeonpea and wheat grown in a rotation (1998)
    Springer
    Nutrient cycling in agroecosystems 51 (1998), S. 225-229 
    Details
    ISSN: 1573-0867
    Keywords: direct ; residual and cumulative P application ; grain yield ; P uptake ; P use efficiency ; pigeon pea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments were conducted for three years to investigate the effect of direct, residual and cumulative P application on the grain yield, P removal and P use efficiency of pigeonpea and wheat grown in rotation. Four levels of P, i.e. 0, 13, 26, and 39 kg P ha-1 were applied to either pigeonpea (Cajanus cajan L., cv. AL 15) or wheat (Triticum aestivum, cv. WL 711) or to both crops. Maximum increase in wheat grain yield ranged from 1.5 to 1.9 t ha-1 and in pigeonpea from 0.28 to 0.36 t ha-1. On average, the grain yield of pigeonpea was 8–15% higher in the residual P treatment than by direct application. In wheat, cumulative P application (to both crops) resulted in highest grain yield, but it did not differ significantly from the direct effect. However, the residual effect of P (applied to previous pigeonpea) on wheat proved to be significantly inferior to direct application. Percent recovery of P from applied fertilizer decreased with P level. Total recovery in the complete rotation was higher where P was directly applied to wheat compared to pigeonpea. The P use efficiency was also highest in the treatment comprising direct application to wheat. Pigeonpea could utilize the residual P more efficiently, indicating that fresh application to this crop can be omitted without any reduction in yield.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009764503975
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  • 110
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    Wheat quality requirements of Asian foods (1998)
    Springer
    Euphytica 100 (1998), S. 155-156 
    Details
    ISSN: 1573-5060
    Keywords: wheat ; quality ; noodles ; steamed bread ; dumplings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Australian wheat quality research has focused on the quality requirements of Asian markets for more than 20 years. This has included the development of appropriate test methods for the laboratory manufacture and quality assessment of wheat-based food products. Products considered have included white salted and yellow alkaline noodles, instant noodles, steamed bread and dumplings. The research has also been aimed at the wheat quality requirements of these products to assist in defining appropriate quality objectives for Australian wheat breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018305418365
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  • 111
    Electronic Resource
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    Screening for zinc efficiency among wheat relatives and their utilisation for alien gene transfer (1998)
    Springer
    Euphytica 100 (1998), S. 281-286 
    Details
    ISSN: 1573-5060
    Keywords: zinc efficiency ; zinc deficiency ; alien chromosome transfer ; cereals ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic diversity for micronutrient efficiency among the most highly adapted and advanced hexaploid and tetraploid wheat cultivars in the world is limited compared with alien species of wheat or rye. Therefore, screening for zinc efficiency was conducted in greenhouse experiments under controlled conditions, and in field trials. Different varieties of hexaploid wheat, hexaploid oats and diploid rye, together with hexaploid and octoploid triticales, wheat-Agropyron, wheat-Aegilops and several wheat-alien chromosome addition series were studied. Considerable differences in zinc efficiency were found between wheat and its relatives. Individual chromosomes of Secale, Agropyron and Haynaldia were found to carry major genes for this character. The transfer of alien chromosome segments was effective, demonstrated using several wheat-rye translocation lines. Alien genetic information was clearly expressed in the wheat genetic background. Further experimental introgressions by chromosome manipulation and marker-aided selection may efficiently contribute to wheat improvement in marginal soils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018376827876
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  • 112
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    The influence of photoperiod genes on the adaptability of European winter wheats (1998)
    Springer
    Euphytica 100 (1998), S. 385-394 
    Details
    ISSN: 1573-5060
    Keywords: wheat ; photoperiod response ; adaptability ; fertility ; linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Photoperiod response genes play a major role in determining the climatic adaptability of European wheat varieties. Photoperiod insensitivity, in the vast majority of photoperiod insensitive European wheat varieties, is probably determined by a Ppd1 allele originally derived from the old Japanese variety Akakomugi. Analysis of the pleiotropic effects of a Ppd1 allele from the Italian variety Mara shows that, besides accelerating ear emergence time, Ppd1 also reduces plant height, tillering, and spikelet numbers. Increases in spikelet fertilities more than compensate for reduced spikelet numbers, producing increased numbers of grains per ear. In southern Europe, early flowering Ppd1 genotypes produce larger grain and greater yields. In England and Germany, pleiotropic effects of Ppd1 on yield vary annually, depending on prevailing weather conditions, from +9% to -16%, over a 10 year period in the United Kingdom. A possible alternative Ppd1 allele from the CIMMYT variety Ciano 67 was compared to that from Mara. Differences associated with complete substituted chromosomes were found to be due to linked genes rather than different Ppd1 alleles. Examination of an alternative weaker gene for photoperiod insensitivity, Ppd2, shows this to exert similar but less significant pleiotropic effects to Ppd1. In the UK, in each of three years of trialing, Ppd2 increased yield 6% more than Ppd1. Results of 10 years trialing show that in Central European countries, between areas where photoperiod sensitive or photoperiod insensitive varieties have a clear adaptive significance, the annual variations in climate make it extremely difficult for breeders to produce varieties with good adaptability to changing environmental conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018327700985
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  • 113
    Electronic Resource
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    Chromosomal locations in common wheat of three new leaf rust resistance genes from Triticum monococcum (1998)
    Springer
    Euphytica 101 (1998), S. 127-131 
    Details
    ISSN: 1573-5060
    Keywords: leaf rust ; Puccinia recondita f. sp. tritici ; resistance genes ; Triticum aestivum ; Triticum monococcum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Monosomic analysis was conducted to determine chromosomal locations of three new leaf rust resistance genes recently transferred to common wheat (Triticum aestivum) from T. monococcum. The resistance gene in wheat germplasm line KS92WGRC23 was transferred from T. monococcum ssp. monococcum. The resistance genes found in KS93U3 and KS96WGRC34 were transferred from T. monococcum ssp. aegilopoides. Allelism tests showed that the three resistance genes were unlinked. The three lines were crossed with each of the seven A-genome Wichita monosomic lines. The leaf rust resistance genes in KS92WGRC23, KS93U3, and KS96WGRC34 were located on chromosomes 6A, 1A, and 5A, respectively, by monosomic analysis. These results demonstrate that the three new genes derived from T. monococcum are each different. They also differ from previously reported Lr genes. This information on chromosome location and the development of mapping populations will facilitate molecular tagging of the new genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018376315133
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  • 114
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    An extended deletion map of the Lr19 translocation and modified forms (1998)
    Springer
    Euphytica 103 (1998), S. 95-102 
    Details
    ISSN: 1573-5060
    Keywords: gene transfer ; physical mapping ; RFLPs ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A physical deletion map of the Lr19 translocated chromosome segment was extended by mapping three additional Thinopyrum RFLP loci. The relative locations of the marker loci on the translocated segment were determined as: centromere, Sd1, Xpsr165, Xpsr105, Xpsr129, XcsIH81-1, Xwg380, Xmwg2062, Lr19, Wsp-D1, Sr25/Y. Various recombinants, putative recombinats and mutants of the Lr19 segment were also characterised with respect to the additional markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018370718296
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  • 115
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    Chromosomal location of genes for supernumerary spikelet in bread wheat (1998)
    Springer
    Euphytica 103 (1998), S. 109-114 
    Details
    ISSN: 1573-5060
    Keywords: branched spike ; gene location ; monosomic analysis ; supernumerary spikelets ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The supernumerary spikelet (SS) character of bread wheat (Triticum aestivum L.) is an abnormal spike morphology expressing extra spikelets per spike. Chromosomal location of the genes for the SS character in the bread wheat line, Yupi Branching was determined by monosomic analysis. The normal-spiked bread wheat Chinese Spring monosomic series were used as testing lines. Data indicated that chromosomes 2D, 4A, 4B and 5A of bread wheat carry genes for SS character (bh genes). Among them, the gene on chromosome 2D has the strongest effect on the expression of the SS character. Comparison of disomic and monosomic plants in 2D, 4A, 4B and 5A F2 populations revealed that the bh genes are hemizygous-effective and dosage-independent. The F1 monosomic analysis showed that the bh genes of Yupi Branching are recessive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018323310621
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  • 116
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    Genetic variation in barley of crossability with wheat and its quantitative trait loci analysis (1998)
    Springer
    Euphytica 103 (1998), S. 187-193 
    Details
    ISSN: 1573-5060
    Keywords: barley ; crossability ; hybridization ; mapping ; quantitative trait loci ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To study genetic variation in crossability, 80 barley accessions of diverse geographic origin consisting of 50 wild barleys (H. vulgare ssp. spontaneum or ssp. agriocrithon) and 30 cultivated barleys (H. vulgare ssp. vulgare) were crossed as the male parent with a highly crossable wheat variety, Shinchunaga. Crossabilities, expressed as the percentage of pollinated florets giving embryo-containing caryopses, ranged from 0% to 68.6%. Barley accessions from East Asia had generally a low crossability, while barley accessions from other regions exhibited a wider range of crossability including highly crossable genotypes. No significant difference in mean crossability was found between wild and cultivated barleys. To estimate the number and location of barley genes controlling the crossability, doubled haploid lines derived from the cross between the barley varieties Steptoe and Morex were crossed as the male parent with wheat. Quantitative trait loci (QTL) analysis using molecular markers identified four QTL. These were mapped to the centromeric regions of chromosomes 2H, 3H and 5H and the short arm of chromosome 7H. The QTL on chromosomes 3H and 5H had larger effects than those on chromosomes 2H and 7H. The four QTL collectively explained 35.4% of the total variance under a multiple QTL model. Relationships of the QTL identified in the present study with previously reported crossability genes of barley and wheat are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018344119747
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  • 117
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    Leaf primordia initiation, leaf emergence and tillering in wheat (Triticum aestivum L.) grown under low-phosphorus conditions (1998)
    Springer
    Plant and soil 202 (1998), S. 149-157 
    Details
    ISSN: 1573-5036
    Keywords: leaf expansion rate ; phosphorus ; phyllochron ; plastochron ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In two simultaneous experiments we examined the effects of phosphorus (P) supply on leaf area development in wheat (Triticum aestivum L.) grown in sand with nutrient solutions. In Experiment 1 we studied leaf emergence, leaf elongation, tiller emergence, shoot growth, and P uptake under four levels of P supply (mM) 0.025 (P1), 0.05 (P2), 0.1 (P3), and 0.5 (P4), and. In Experiment 2 there were two levels of P supply, P1 and P4, and we examined the effects of P on leaf primordia differentiation and leaf emergence. The phyllochron was calculated as the inverse of the rate of leaf emergence calculated from the regression of number of leaf tips (PHY-Ltip), Haun index (PHY-Haun), and as the cumulated thermal time between the emergence of two consecutive leaves (PHYtt). The plastochron was calculated from the inverse of the rate of leaf primordia initiation in the apex. P deficiency delayed the emergence of leaves on the main stem and on the tiller 1. Phosphorus deficiency increased the time from emergence to double ridge and anthesis. The final number of leaves was not affected by P. The effects of P on the value of the phyllochron were attributed to both a reduced rate of leaf primordia initiation, and to a reduced leaf elongation rate. P deficiency delayed or even suppressed the emergence of certain tillers. In this work a phosphorus deficiency that reduced shoot growth by 25% at 44 days after emergence significantly modified the structure of the plants by increasing the value of the phyllochron and delaying tillering. These results suggest that any attempt to simulate leaf area development and growth of wheat plants for P-limited conditions should include the effects of the deficiency on leaf emergence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004352820444
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  • 118
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    Plant leaf area expansion and assimilate production in wheat (Triticum aestivum L.) growing under low phosphorus conditions (1998)
    Springer
    Plant and soil 200 (1998), S. 227-240 
    Details
    ISSN: 1573-5036
    Keywords: leaf expansion rate ; modelling ; phosphorus ; photosynthesis ; phyllochron ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Under phosphorus deficiency reductions in plant leaf area have been attributed to both direct effects of P on the individual leaf expansion rate and to a reduced availability of assimilates for leaf growth. In this work we use experimental and simulation techniques to identify and quantify these processes in wheat plants growing under P-deficient conditions. In a glasshouse experiment we studied the effects of soil P addition (0–138 kg P2O5 ha-1) on tillering, leaf emergence, leaf expansion, plant growth, and leaf photosynthesis of wheat plants (cv. INTA Oasis) that were not water stressed. Plants were grown in pots containing a P-deficient (3 mg P g-1 soil) sandy soil. Sowing and pots were arranged to simulate a crop stand of 173 plants m-2. Experimental results were integrated in a simulation model to study the relative importance of each process in determining the plant leaf area during vegetative stages of wheat. Phosphorus deficiency significantly reduced plant leaf area and dry weight production. Under P-deficient conditions the phyllochron (PHY) was increased up to a 32%, compared to that of high-P plants. In low-P plants the rate of individual leaf area expansion during the quasi-linear phase of leaf expansion (LER) was significantly reduced. The effect of P deficiency on LER was the main determinant of the final size of the individual leaves. In recently expanded leaves phosphorus deficiency reduced the photosynthesis rate per unit leaf area at high radiation (AMAX), up to 57%. Relative values of AMAX showed an hyperbolic relationship with leaf P% saturating at 0.27%. Relative values of the tillering rate showed an hyperbolic relationship with the shoot P% saturating at values above 0.38%. The value of LER was not related to the concentration of P in leaves or shoots. A morphogenetic model of leaf area development and growth was developed to quantify the effect of assimilate supply at canopy level on total leaf area expansion, and to study the sensitivity of different model variables to changes in model parameters. Simulation results indicated that under mild P stress conditions up to 80% of the observed reduction in plant leaf area was due to the effects of P deficiency on leaf emergence and tillering. Under extreme P-deficient conditions the simulation model failed to explain the experimental results indicating that other factors not taken into account by the model, i.e. direct effects of P on leaf expansion, must have been active. Possible mechanisms of action of the direct effects of P on individual leaf expansion are discussed in this work.
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    Relationship between anther culture response and aluminium tolerance in wheat (Triticum aestivum L.) (1998)
    Springer
    Euphytica 100 (1998), S. 249-252 
    Details
    ISSN: 1573-5060
    Keywords: wheat ; triticale ; aluminium ; anther culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A combination of in vivo and in vitro selection methods were used to increase aluminium tolerance in wheat using wheat x triticale crosses. Both in vivo and in vitro aluminium treatments significantly influenced the anther culture response. In vivo selection at the seedling stage resulted in significantly higher embryo induction. On induction media containing aluminium, the embryoid induction frequency dropped significantly, but there was an increase in the green plant regeneration frequency. In spite of this effect, all doubled haploid (DH) lines were more tolerant to aluminium in seedling tests than the winter wheat parent. The application of in vivo aluminium selection, before the start of anther culture, increased the probability of obtaining DH lines with significantly higher tolerance, compared to the original population. After three selection cycles of the original populations, there was a significant difference in the root regrowth rate of tolerant and sensitive plants. Both sensitive and tolerant plants showed a decrease due to the presence of aluminium in the induction media, with a greater decrease occurring in sensitive plants. Correlation between the rate of root regrowth in the seedling test and the change in embryo induction was positive, but moderate, emphasising the fact that plants with higher root regrowth tended to be more tolerant of the presence of aluminium in the induction medium.
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    The use of wheat-alien and Aegilops-rye amphiploids for introgression of genetic material to wheat (1998)
    Springer
    Euphytica 100 (1998), S. 313-321 
    Details
    ISSN: 1573-5060
    Keywords: alien gene transfer ; amphiploids ; chromosome number distribution ; chromosome pairing ; wheat ; wide hybridisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An introduction of genetic material from rye, Aegilops and Elymus into durum and common wheat by crossing the wheat species with different amphiploids, has been attempted. Meiotic studies of the hybrids demonstrated that the wheat Elymus sibiricus amphiploid contained several (two or three) genes suppressing the activity of the wheat homoeologous pairing control system. Somatic chromosome studies of the hybrids revealed that the distributions of the alien chromosomes transferred to the second hybrid generation did not correspond to random (0.5 + 0.5)2k and binomial (p + q)2k distributions. An essential amendment for gamete and zygote viability, allowing the approximation of distributions by binomial equation, is discussed. The preferential E. sibiricus chromosome transmission was observed. The first backcross was found to be a critical stage while using the Aegilops-rye amphiploids for production of wheat introgressive forms. Stabilisation of the somatic karyotype and improvement of the meiotic regularity was observed in a number of generations. The isolation of stable cytological lines in BC3 was found to be possible.
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    Photoperiod and vernalisation response of Mediterranean wheats, and implications for adaptation (1998)
    Springer
    Euphytica 100 (1998), S. 377-384 
    Details
    ISSN: 1573-5060
    Keywords: adaptation ; photoperiod ; vernalisation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hexaploid wheat has the largest cultivated area among crop plants due to its adaptability to different agroclimatic regions. A large part of this adaptability depends upon the variation in vernalisation and photoperiod requirements. A better understanding of the genetic control of flowering in wheat, as expressed by vernalisation requirements and photoperiod response, will guide breeders in targeting crosses of different types and will also improve our understanding of regional adaptation requirements. Characterisation of large numbers of breeding lines for photoperiod and vernalisation response in wheat is needed to assign the lines to geographic areas of most probable adaptation. Simple screening methods to quantify the effects of these two factors and their interaction are needed to assist breeding progress. Twenty wheat lines were evaluated for response to photoperiod and vernalisation under two controlled environments and under high ambient air temperatures in field conditions. Vernalised and non vernalised seedlings were transplanted into pots and placed in three photoperiod (8, 12 and 16 h light) cabinets, in the greenhouse or in growth chambers. Days to anthesis decreased with increasing length of photoperiod. Vernalised plants flowered earlier than non vernalised plants. There was a significant correlation between days to anthesis in the greenhouse and the growth chamber (r=0.88, P〈0.001). Length of basal vegetative period, effects of vernalisation, and photoperiod from the two screening techniques were positively correlated with each other. Growth habit score, vernalisation requirement and heading date in the field were highly correlated with the main effect of vernalisation in the two controlled environments. The results indicated that selection for vernalisation response in a large number of genotypes can be achieved under high ambient air temperatures in the field. The selected material can subsequently be screened for photoperiod response under greenhouse conditions. Using these techniques, 49 local and improved cultivars from the Mediterranean region in west Asia and north Africa (WANA), showing differences in response to photoperiod, vernalisation, and earliness independent of vernalisation and photoperiod, affecting time to anthesis, were identified. Most old local cultivars were sensitive to both photoperiod and vernalisation. All the improved genotypes were insensitive to photoperiod. Responses to vernalisation were generally small under short photoperiods, but were more pronounced in long photoperiod, particularly in winter and facultative types from northern latitudes. These results should help to explain the adaptability of cultivars based on photoperiod and vernalisation requirements and their interaction.
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    RFLP mapping of the three major genes, Vrn1, Q and B1, on the long arm of chromosome 5A of wheat (1998)
    Springer
    Euphytica 101 (1998), S. 91-95 
    Details
    ISSN: 1573-5060
    Keywords: chromosome 5A ; RFLP mapping ; recombinant line ; vernalization gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Chromosome 5A of wheat carries several major genes of agronomic importance, including Vrn1 controlling spring/winter wheat difference, Q determining spike morphology and B1 inhibiting awn development. A population of single-chromosome recombinant lines from the cross between two chromosome substitution lines, 'Chinese Spring' (Cappelle-Desprez 5A) and 'Chinese Spring' (Triticum spelta 5A) was developed to map these genes on the long arm of chromosome 5A relative to RFLP markers. Using 120 recombinant lines, a map of approximately 230 cM in length was constructed. The gene order was centromere– Vrn1– Q– B1. The Vrn1 locus was tightly linked to two RFLP markers, Xbcd450 and Xrz395 with 0.8 cM, and to Xpsr426 with 5.0 cM. The Vrn1-adjacent region was located in the central of the long arm, approximately 90 cM from the centromere. The chromosome region around Q and the 5A/4A translocation break-point were mapped by three RFLP markers, and their order was found to be Q– Xpsr370– Xcdo457–4A/5A break-point– Xpsr164. The B1 locus was located on the most distal portion of the long arm.
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    DNA profiling and plant variety registration. III: The statistical assessment of distinctness in wheat using amplified fragment length polymorphisms (1998)
    Springer
    Euphytica 102 (1998), S. 335-342 
    Details
    ISSN: 1573-5060
    Keywords: AFLP ; distinctness testing ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The use of AFLP analysis to produce DNA profiles from a set of 55 wheat varieties, commonly grown in the UK over the past 60 years, is described. Using six different primer pairs, 90 polymorphic bands were readily recognised and recorded. These AFLP bands are not significantly clustered and hence can be used with some confidence, even though they are not mapped. Statistical approaches to the analysis of the data were developed such that the discrimination between the varieties achieved by the use of the six primer pairs, both separately and in combination, could be derived and compared to that achieved by a common set of morphological descriptors. Various criteria for the definition of distinctness in terms of the number of band differences required between pairs of varieties were also compared. In general, higher levels of discrimination were achieved by the inclusion of greater numbers of bands in the analysis. The optimal number of polymorphic bands appears to be between v and 2v, where v is the number of varieties under test. Discrimination levels were adversely affected if the number of bands was below v/2. Distinctness levels achieved by the use of molecular markers can be calibrated so that they reproduce those seen with morphological characters. The results are discussed in relation to the possible use of DNA profiling methods for distinctness, uniformity and stability testing.
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    Effect of parental genotypes on haploid embryo and plantlet formation in wheat × maize crosses (1998)
    Springer
    Euphytica 103 (1998), S. 319-323 
    Details
    ISSN: 1573-5060
    Keywords: interspecific cross ; haploid ; wheat ; maize ; rye chromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genotypic influence of both male and female parents on haploid production through interspecific crosses was studied using eight wheat and four maize genotypes. The average numbers of embryos and green haploid plantlets obtained per pollinated floret were 17.6% and 10.1%, respectively. Clear genotypic influence of the wheat genotype was detected, but heterozygosity of the wheat did not affect haploid production. Analogous response to anther culture and interspecific crossing was observed, still a wheat variety which did not respond to anther culture, produced 1.1 plantlets per pollinated spike upon maize pollination. This appears to be a major advantage of interspecific crossing compared to anther culture technique in wheat. Circumstantial evidence is presented for specific wheat × maize interaction on haploid plantlet formation. Rye chromatin enhanced haploid production but only in a complete 1B/1R substitution line. Ovaries with an embryo were found to be dispersed evenly all over the wheat spike, suggesting that within certain limits the developmental stage of ovaries and thus time of pollination within a spike are not as important as it was previously assumed.
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    Fast changes in growth rate and cytokinin content of the shoot following rapid cooling of roots of wheat seedling (1998)
    Springer
    Plant growth regulation 26 (1998), S. 105-108 
    Details
    ISSN: 1573-5087
    Keywords: cooling ; cytokinins ; growth ; immunoassay ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Changes in the concentration of cytokinins were studied following root cooling. Simultaneously, the growth rate of the second leaf was monitored with a highly sensitive growth sensor attached to its tip. Cytokinins were separated by thin layer chromatography and immunoassayed using antibodies to zeatin riboside. The extension rate of the second leaf decreased within 15 minutes of cooling the nutrient medium from 24 °C to 4 °C. The concentration of cytokinins in shoots decreased with similar rapidity. In contrast cytokinins in roots increased slightly during the initial period of cooling before declining. The sharp decrease in cytokinin concentrations in shoots 15 minutes after cooling of roots may contribute to the abrupt inhibition of shoot growth.
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    Statistical properties of chlorophyll fluorescence induction parameters (1998)
    Springer
    Photosynthetica 35 (1998), S. 121-127 
    Details
    ISSN: 1573-9058
    Keywords: Gaussian distribution ; leaf age ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Parameters of the fast chlorophyll (Chl) fluorescence induction (the O-J-I-P curve) of plants of winter wheat grown in the field canopy were statistically tested for Gaussian distribution. Five different statistical methods showed that the obtained values did not obey the Gaussian distribution law. The presentation of the parameters with the help of the mean and standard deviation masks the information about statistical properties of the values. Thus, we recommend to present the parameters by means of median, quartiles, and minimum and maximum values rather than by means of the mean and standard deviation.
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    URL: http://dx.doi.org/10.1023/A:1006886202444
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    Effect of Organic Materials on Partitioning, Extractability and Plant Uptake of Metals in an Alum Shale Soil (1998)
    Springer
    Water, air & soil pollution 103 (1998), S. 405-421 
    Details
    ISSN: 1573-2932
    Keywords: alum shale ; Cd ; Cu ; extractability ; metal partitioning ; Ni ; Norway ; organic materials ; plant uptake ; wheat ; Zn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Soils developed on sulphide-bearing shale (alum shale) in Norway contain naturally high amount of heavy metals. We conducted a greenhouse pot experiment to study the effect of four rates (0, 2, 4, and 8%) and three sources (cow manure, pig manure and peat soil) of organic matter in partitioning and distribution, extractability and plant uptake of Cd, Cu, Ni and Zn in an alum shale soil. Sequential extraction scheme was used to determine the distribution patterns of metals in the soil. DTPA was used for extracting the metals from the soil. Wheat (Triticum aestivum) was used as a test crop to study the plant uptake of metals. The highest amount of Cd was present in the exchangeable fraction, irrespective of the rate and source of organic matter applied. Copper, Ni, and Zn, on the other hand, were present only in small quantities in this fraction. The largest fraction of Cu was associated with organic matter and the amounts present in the oxide, carbonate and exchangeable fractions were very small. Nickel and Zn were found mainly in the residual fraction. Increasing rates of cow and pig manure decreased the amounts of Cd and Ni associated with the exchangeable fraction whereas, the addition of peat soil at the same rate increased the amounts of these metals associated with this fraction. This effect of organic matter was primarily associated with the change in soil pH caused by different organic matter sources. The DTPA-extractable metals were decreased with increasing rates of organic matter application, irrespective of its source. Grain and straw yields of wheat were decreased with increasing rates of organic matter. The application of organic matter increased the Cu and Zn concentrations in both grain and straw. The concentration of all metals was lower in plants grown in the cow manure amended soil as compared to those grown in the soil amended with either pig manure or peat soil. These results sugggest that the source of organic matter was a determining factor for metal distribution in the soil and for metal uptake by plants. In this study cow manure slightly increased the soil pH and thus was more effective than either pig manure or peat soil in reducing the plant uptake of metals but in general the efficiency of the organic material in reducing heavy metal uptake was small.
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    Use of a lux-based procedure to rapidly visualize root colonisation by Pseudomonas fluorescens in the wheat rhizosphere (1997)
    Springer
    Antonie van Leeuwenhoek 72 (1997), S. 365-372 
    Details
    ISSN: 1572-9699
    Keywords: bioluminescence ; Pseudomonas ; root colonization ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bioluminescently marked Pseudomonas fluorescens strain 5RL, has been used previously to follow colonisation of soy bean roots (De Weger et al. [1991] Appl. Environ. Microbiol. 57:36-41). In the present paper the method has been further developed and optimized for wheat roots and it is used to get a quick overview of the colonisation patterns of many different root systems at the same time. Colonisation was followed on wheat plants grown in our gnotobiotic sand system (Simons et al., 1996. Mol Plant Microbe Interact 9: 600–607) and the following results were obtained. (i) A spatio-temporal analysis of the colonisation of wheat roots showed that 4 days after planting the highest bacterial activity was observed at the upper part of the root. After 6 days the high bacterial activity at the upper part was further increased, whereas spot-like activities were observed on the lower root parts, possibly due to micro-colonies. (ii) Bacterial mutations causing lack of motility or auxotrophy for amino acids resulted in impaired colonisation of the lower root parts, indicating that motility and prototrophy for the involved amino acid(s) are important factors for wheat root colonisation by strain 5RL. (iii) Coinoculation of strain 5RL with other wild type Pseudomonas strains on the root influenced the colonisation pattern observed for strain 5RL. Colonisation was not visually affected when the competing strain was a poor root coloniser, but was severely reduced when the competing strain was a good root coloniser. The results show that the spatio-temporal colonisation of wheat root by P. fluorescens strain 5RL and derivatives is similar to that of strain WCS365 on tomato. The advantage of the use of lux-marked strains is that the results are obtained much quicker than when conventional methods are used and that the result is supplied as an image of the colonisation pattern of many different roots.
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    Characteristics of Hydroxamic Acid Induction in Wheat Triggered by Aphid Infestation (1997)
    Springer
    Journal of chemical ecology 23 (1997), S. 2695-2705 
    Details
    ISSN: 1573-1561
    Keywords: Defense ; herbivory ; aphids ; wheat ; Gramineae ; hydroxamic acids ; Defense theory ; Carbon/Nutrient theory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Hydroxamic acids (Hx) are natural products of Gramineae that are associated with cereal resistance to pests. We aimed at characterizing the induction of Hx accumulation in seedlings of wheat,Triticum aestivum, by short-term infestation of the cereal aphid,Rhopalosiphum padi. A load of 25 aphids increased significantly the Hx levels in the infested primary leaf in comparison with control levels. Lower loads did not increase Hx concentration. Aphid infestation lasting 16 hr did not elicit induction of Hx, even after a time-lag of 32 hr to allow the expression of any induced response. Forty-eight hours was the minimum duration of aphid infestation required to trigger Hx induction. The age of the infested tissue (the primary leaf) did not affect induction. Similar increases of Hx were found in unfolding, expanding, and totally expanded primary leaves. It was determined that the regime of nutrient supply (N-intensive nutritive solutions at low and high concentration) to wheat seedlings had no effect on the magnitude of the aphid-induced Hx (N-based secondary metabolites). Results obtained are discussed in the framework of general theories of plant defense allocation.
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    Expression of a wheat ADP-glucose pyrophosphorylase gene during development of normal and water-stress-affected anthers (1997)
    Springer
    Plant molecular biology 34 (1997), S. 445-453 
    Details
    ISSN: 1573-5028
    Keywords: ADP-glucose pyrophosphorylase ; anther ; pollen ; male sterility ; water stress ; wheat ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In wheat (Triticum aestivum L.), water deficit during meiosis in the microspore mother cells (MMCs) induces pollen abortion, resulting in the failure of fertilization and a reduction in grain set. In stressed plants, meiosis in MMCs proceeds normally but subsequent pollen development is arrested. Unlike normal pollen grains, which accumulate starch during the late maturation phase, stress-affected anthers contain pollen grains with little or no starch. Stress also alters the normal distribution of starch in the anther wall and connective tissue. To determine how starch biosynthesis is regulated within the developing anthers of stressed plants, we studied the expression of ADP-glucose pyrophosphorylase (AGP), which catalyzes the rate limiting step of starch biosynthesis. Two partial-length cDNAs corresponding to the large subunit of AGP were amplified by RT-PCR from anther RNA, and used as probes to monitor AGP expression in developing anthers of normal and water-stressed plants. These clones, WAL1 and WAL2, had identical deduced amino acid sequences and shared 96% sequence identity at the nucleic acid level. In normal anthers, AGP expression was biphasic, indicating that AGP expression is required for starch biosynthesis both during meiosis and later during pollen maturation. AGP expression in stressed anthers was not affected during the first phase of starch accumulation, but was strongly inhibited during the second phase. We conclude from these results that the reduced starch deposition later in the development of stressed pollen could be the result of a lower expression of AGP. However, this inhibition of AGP expression is unlikely to be the primary cause of male sterility because anatomical symptoms of pollen abortion are observed prior to the time when AGP expression is inhibited.
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    Characterization of dynamics of the psbD light-induced transcription in mature wheat chloroplasts (1997)
    Springer
    Plant molecular biology 33 (1997), S. 267-278 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; in vitro transcription ; light ; psbA ; psbD/C operon ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dynamical aspects of three chloroplast promoters responding to change in light condition were examined in mature chloroplasts of wheat (Triticum aestivum) by in vitro transcription. The wheat psbD/C operon has four distinct promoters, two of which named as D/C-3 and D/C-4 promoters dominantly function in mature chloroplasts to produce the mRNAs encoding D2/CP43 and CP43 alone, respectively. Activity of the D/C-3 promoter in mature chloroplasts was reduced to less than 30% by 24 h dark adaptation and recovered by re-illumination to the original level within 30 to 60 min. The activation of the D/C-3 promoter which requires de novo cytoplasmic protein synthesis was induced by low fluence of light (e.g. 16 µE m-2 s-1), but the extent of activation increased with increasing light fluence. The accumulation of mRNAs from the D/C-3 promoter saturated at 2- to 3-fold higher level within 2 h when the dark-adapted seedlings were transferred to the lig at 72 µE m-2 s-1, concomitant with the increase in rate of D2 synthesis, suggesting that synthesis of D2 in mature chloroplasts is controlled via the D/C-3 promoter activity in a light-dependent way. Activity of the D/C-4 promoter slightly increased in the dark and decreased in the light. Effect of light on the psbA promoter activity was not observed at all in mature chloroplasts. In vitro transcriptional analysis of the D/C-3 promoter with 5′ deletion mutations revealed that at least two cis elements which are located within the sequences of -78 to -47 and -46 to -29 of the transcription initiation site, respectively, act as enhancing elements in the D/C-3 promoter. The light-switching element of the transcription, however, was suggested to be located in the core promoter sequence downstream of the -35 element.
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    The wheat protein kinase gene, TaPK3/, of the PKABA1 subfamily is differentially regulated in greening wheat seedlings (1997)
    Springer
    Plant molecular biology 33 (1997), S. 935-941 
    Details
    ISSN: 1573-5028
    Keywords: cold temperature ; dehydration ; greening ; protein kinase ; Triticum aestivum/ ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have identified a new wheat PKABA1/-like protein kinase gene, TaPK3/, that is expressed in greening wheat seedlings. TaPK3 has high sequence homology (97% similarity with some sequence diversity at the 3' end) to the wheat PKABA1 protein kinase mRNA, which is upregulated by cold-temperature treatment, dehydration and abscisic acid (ABA). Use of a TaPK3 gene-specific probe has revealed that TaPK3 is differentially expressed with respect to PKABA1. TaPK3 mRNA accumulates in greening shoot tissue of wheat, but is not affected by dehydration, cold-temperature treatment or ABA. Based on sequence and expression differences, we conclude that expression of the PKABA1/-like protein kinases is not limited to stress responses.
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    cDNA structure and expression patterns of a low-temperature-specific wheat gene tacr7 (1997)
    Springer
    Plant molecular biology 34 (1997), S. 643-650 
    Details
    ISSN: 1573-5028
    Keywords: callus ; crown tissue ; gene expression ; low temperature ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The low-temperature (2 °C)-specific wheat cDNA, pTACR7, represents a gene designated tacr7 from hard red winter wheat (HRWW; Triticum aestivum L. cv. Winoka). The term low-temperature-specific (LTS) is used because tacr7 is not induced by ABA or stresses such as salt, dehydration, and heat. pTACR7 was isolated by RT-PCR with mRNA from wheat crown tissue, the oligonucleotide primers derived from the barley cognate pHVCR8 (GenBank accession number L28091). Based on the deduced amino acid sequence, TACR7 is highly hydrophobic, with a single transmembrane domain and an amino acid bias for leucine (19%). Thus, the encoded protein TACR7 is unique among low-temperature-regulated wheat proteins described in the literature. Analysis of steady-state levels of tacr7 transcripts (630 nt) showed accumulation in wheat seedlings, crown tissue, and callus cultures after transfer from control (25 °C) to low temperature (2 °C). No detectable transcripts were observed by northern blot hybridization with pTACR7 probe from seedling or callus treated with ABA, salt, dehydration, or heat stress. tacr7 transcripts accumulated during 2 °C exposure to a greater amount in a freeze-resistant HRWW (FR; SDmut 16029) than in a freeze-susceptible HRWW (FS; SDmut 16169) crown tissue, with the largest difference between genotypes being 30% ± 3% at 3 weeks.
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    Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds (1997)
    Springer
    Molecular breeding 3 (1997), S. 65-74 
    Details
    ISSN: 1572-9788
    Keywords: leaf rust ; molecular marker ; receptor-like kinase ; resistance breeding ; resistance gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We recently showed that the Lr10 wheat leaf rust resistance gene cosegregated with the candidate resistance gene Lrk10 which encodes a putative receptor-like kinase. The aim of this study was to develop Lrk10-derived molecular markers for the detection of the Lr10 gene in breeding material. Different subfragments of Lrk10 were tested as RFLP markers for the Lr10 resistance gene. The most specific fragment (Lrk10-6) was converted into the PCR-based STS marker STSLrk10-6. Both the RFLP and the STS marker did not give a signal with near isogenic lines containing a different Lr gene. The applicability of these markers for the detection of Lr10 in genetically diverse material was tested with 62 wheat and spelt breeding lines, mostly from European breeding programmes. Twelve varieties known to have Lr10 showed the same alleles as the originally characterized line ThatcherLr10. Most of the lines with unknown composition at the Lr10 locus had a null allele with both the RFLP marker Lrk10-6 and the marker STSLrk10-6 whereas 20% of the lines had a different allele. For six lines, including a traditional spelt variety derived from a landrace, both markers showed the same allele as Thatcher Lr10. Artificial infections of these lines with an isolate avirulent on Lr10 resulted in a hypersensitive reaction of all these lines, indicating also the presence of the Lr10 resistance gene. These data demonstrate that the markers derived from sequences of Lrk10 are highly specific for the Lr10 gene in breeding material of very diverse genetic origin. The markers will allow the defined deployment of Lr10 in wheat breeding programmes and will contribute to the elucidation of the role of Lr10 in polygenic resistances against leaf rust.
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    URL: http://dx.doi.org/10.1023/A:1009619905909
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    A molecular marker associated with the H21 Hessian fly resistance gene in wheat (1997)
    Springer
    Molecular breeding 3 (1997), S. 177-181 
    Details
    ISSN: 1572-9788
    Keywords: bulked segregant analysis ; H21 ; near-isogenic lines (NILs) ; RAPD ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Near-isogenic lines in conjunction with bulked segregant analysis were used to identify a DNA marker in wheat (Triticum aestivum L.) associated with the H21 gene conferring resistance to biotype L of Hessian fly [Mayetiola destructor (Say)] larvae. Near-isogenic lines were developed by backcross introgression BC3F3:4 (‘Coker 797’ * 4 / ‘Hamlet’) and differed by the presence or absence of H21 (on 2RL) derived from ‘Chaupon’ rye (Secale cereale L.). Bulked DNA samples were prepared from near-isogenic lines and BC3F2 population individuals segregating for reaction to Hessian fly biotype L and screened for random amplified polymorphic DNA markers using 46 10mer primers. Random-amplified polymorphic DNA markers from resistant and susceptible individuals and parental lines were scored and these data were used to identify a 3 kb DNA fragment that was related to the occurrence of H21. This fragment was amplified from DNA isolated from Hamlet, a near-isogenic line carrying 2RL, and bulked DNA from resistant BC3F2 individuals, but not from the recurrent parent Coker 797 or DNA bulks from susceptible BC3F2 plants. Analysis of 111 BC3F2 segregating individuals and BC3F2:3 segregants confirmed the co-segregation of the 3 kb DNA marker with the H21 resistance gene to Hessian fly. Use of this marker could facilitate more rapid screening of plant populations for Hessian fly resistance and monitoring the introgression of H21.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009606304447
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    Comparative genetics of flowering time (1997)
    Springer
    Plant molecular biology 35 (1997), S. 167-177 
    Details
    ISSN: 1573-5028
    Keywords: comparative genetics ; rice ; barley ; wheat ; Arabidopsis ; flowering time (heading date) ; photoperiod ; vernalization ; earliness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of genes controlling flowering time (heading date) contributes to our understanding of fundamental principles of plant development and is of practical importance because of the effects of flowering time on plant adaptation and crop yield. This review discusses the extent to which plants may share common genetic mechanisms for the control of flowering time and the implications of such conservation for gene isolation from the major cereal crops. Gene isolation may exploit the small genome of rice in map-based approaches, utilizing the conservation of gene order that is revealed when common DNA markers are mapped in different species. Alternatively, mechanisms may be conserved within plants as a whole, in which case genes cloned from the model dicot Arabidopsis thaliana provide an alternative route.
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    URL: http://dx.doi.org/10.1023/A:1005726329248
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    Chlorsulfuron reduces rates of zinc uptake by wheat seedlings from solution culture (1997)
    Springer
    Plant and soil 188 (1997), S. 309-317 
    Details
    ISSN: 1573-5036
    Keywords: chlorsulfuron ; nutrient solution ; roots ; uptake ; wheat ; zinc deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat plants differing in zinc efficiency (Excalibur; Zn-efficient, Gatcher and Durati; Zn-inefficient) were grown in HEDTA chelate-buffered nutrient solution in controlled conditions and supplied with 0 or 40 μg chlorsulfuron L-1 . Zinc uptake rates of 12-d-old plants were measured over 80 or 90 minutes using65 Zn added to nutrient solutions. Increasing the zinc concentration of the solution increased the rate of zinc uptake, while the percentage of zinc transported to shoots was decreased. Addition of chlorsulfuron to uptake solutions for 90 minutes did not influence rate of zinc uptake or transport of zinc to shoots. Pretreating plants with chlorsulfuron for 5 days decreased zinc uptake rates, but transport to shoots was proportionally increased. Three-day pretreatment with chlorsulfuron was the minimum required for significant differences in uptake and transport of zinc to occur. Plants exposed to chlorsulfuron for 3 days required a further 5 days of growth in chlorsulfuron-free solutions before uptake rates recovered to control plant rates. It is concluded that chlorsulfuron deleteriously but reversibly affects uptake of zinc across the plasma membrane after prolonged exposure.
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    URL: http://dx.doi.org/10.1023/A:1004256429979
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    Effects of mycorrhizal infection on drought tolerance and recovery in safflower and wheat (1997)
    Springer
    Plant and soil 197 (1997), S. 95-103 
    Details
    ISSN: 1573-5036
    Keywords: drought ; mycorrhizal fungi ; safflower ; water relations ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of arbuscular mycorrhizal fungi on drought tolerance and recovery was studied in safflower (Carthamus tinctorius L.) and wheat (Triticum aestivum L.). Plants were grown with and without the mycorrhizal fungus, Glomus etunicatum Becker & Gerd., in nutrient-amended soil under environmentally-controlled conditions to yield mycorrhizal and nonmycorrhizal with similar leaf areas, root length densities, dry weights, and adequate tissue phosphorus. When drought stress was induced, mycorrhizal infection did not affect changes in leaf water, osmotic or pressure potentials, or osmotic potentials of leaf tissue rehydrated to full turgor in either safflower or wheat. Furthermore, in safflower, infection had little effect on drought tolerance as indicated by the level of leaf necrosis. Mycorrhizal wheat plants, however, had less necrotic leaf tissue than uninfected plants at moderate levels of drought stress (but not at severe levels) probably due to enhanced phosphorus nutrition. To determine the effects of infection on drought recovery, plants were rewatered at a range of soil water potentials from –1 to –4 MPa. We found that although safflower tended to recover more slowly from drought after rewatering than wheat, mycorrhizal infection did not directly affect drought recovery in either plant species. Daily water use after rewatering was reduced and was correlated to the extent that leaves were damaged by drought stress in both plant species, but was not directly influenced by the mycorrhizal status of the plants.
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    URL: http://dx.doi.org/10.1023/A:1004286704906
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    Rooting patterns in near-isogenic lines of spring wheat for dwarfism (1997)
    Springer
    Plant and soil 197 (1997), S. 79-86 
    Details
    ISSN: 1573-5036
    Keywords: dwarfing genes ; root and stem mass ratios ; root dry-weight ; root length ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of Rht alleles on root growth and distribution in isogenic lines of spring wheat (Triticum aestivum L.) are described under different environmental conditions. Above-ground biomass, root length, root dry-weight and their distribution along the soil profile were measured by destructive sampling for growth of aerial biomass and extraction of soil cores containing roots. Field experiments were conducted under non-limiting water and nutritional conditions during two consecutive years, using an early and a late sowing date each year. Dwarfing genes significantly reduced plant height and above-ground biomass at anthesis. In addition, stem mass ratio also was reduced with increases in the allelic dosage. Conversely, total root length and root dry-weight per unit area at anthesis were increased with decreased plant height, therefore, root mass ratio tended to be negatively correlated with plant height. Differences in distribution of root length and root dry-weight through the soil profile among lines were largely confined to the upper soil layers (i.e. the top 30 cm). Differences in root dry-weight were more important than in root length, so that the dwarf line had the highest root mass per unit root length. Furthermore, a significant positive correlation between the root mass ratio and stem mass per unit stem length was found. It is suggested that increases in root mass per unit root length associated with Rht alleles are evidencing a surplus of photoassimilates during stem elongation which are used for thickening the roots due to the lack of alternative sinks. Agronomic implications of this effect are discussed.
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    URL: http://dx.doi.org/10.1023/A:1004207407979
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    Modelling root growth of wheat as the linkage between crop and soil (1997)
    Springer
    Plant and soil 190 (1997), S. 267-277 
    Details
    ISSN: 1573-5036
    Keywords: dynamic model ; root growth ; root impacts ; root-shoot ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The simulation of crop - soil systems with a model requires an appropriate description of the root dynamics. An empirical root growth model that simulates root-shoot relations, root distribution and a dynamic response to environmental conditions is presented. The root model extends an existing crop model and links it to a soil model to calculate dry matter accumulation, water and nitrogen dynamics of a wheat crop. Simulated roots are distributed over soil layers according to carbon supply from the shoots by using a 'top down principle'. This principle favours the top layers for root growth by first providing all available carbon to the first layer. Under unfavourable soil conditions in that layer, carbon is given to the next deeper soil layer. This procedure is repeated until a separately calculated rooting depth is reached. At that depth all available carbon is used for root growth regardless of current soil conditions. Under most simulated conditions the 'top down principle ' results in a negative exponential function of a monotone decrease of root distribution with soil depth. However, it can also account for larger root densities deeper in the profile when water or nitrogen deficiency occurs in soil. In addition to soil water and soil nitrogen supply the root model considers soil compaction, aeration and root distribution history for root growth simulation. The new model, consisting of an existing crop and soil model and linked through a new developed root model, was calibrated and tested using two independent field experiments. A sensitivity analysis was carried out by varying parameters, initial soil conditions and hypothetic weather patterns as part of the validation process. Root length density distribution (r2(1:1)=0.65), shoot, grain and total root biomass (r2(1:1)=0.87) were predicted satisfactorily, thus providing a useful tool for specific simulation studies on that site.
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    URL: http://dx.doi.org/10.1023/A:1004228201299
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    Components of quantitative resistance to yellow rust in ten spring bread wheat cultivars and their relations with field assessments (1997)
    Springer
    Euphytica 96 (1997), S. 215-223 
    Details
    ISSN: 1573-5060
    Keywords: Puccinia striiformis ; Triticum aestivum ; association of components ; stripe rust ; durable resistance ; infection frequency ; latency period ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Latency period, infection frequency, lesion length, lesion growth, disease severity and percentage of infected leaf parts were assessed on 10-day-old seedling leaves and flag leaves of ten bread wheat (Triticum aestivum L.) cultivars after inoculation with urediospores of Puccinia striiformis Westend. f. sp. tritici. For all components significant genotypic differences were detected. Components of resistance tended to be associated. A long latency period was associated with a low infection frequency, small lesions, a low disease severity and a low percentage of infected leaf parts. The latency period, measured as time period until first pustule appearance (LP1), was highly correlated with the latency period measured as time period until 50% of the pustules appeared (LP50). Assessment of latency period of large numbers of cultivars could therefore be reliably done by measuring LP1 which is less time consuming than measuring LP50. Latency period, infection frequency and disease severity were highly correlated with disease development data from field experiments. These results suggest that selection in the greenhouse for one of these components should result in cultivars with high levels of quantitative resistance. Disease severity after uniform inoculation in the greenhouse can be used for monocyclic evaluations because it is the easiest to assess.
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    URL: http://dx.doi.org/10.1023/A:1002916110347
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    Evaluation of resistance to Fusarium head blight in indigenous Japanese species of Agropyron (Elymus) (1997)
    Springer
    Euphytica 97 (1997), S. 39-44 
    Details
    ISSN: 1573-5060
    Keywords: Agropyron ; Elymus ; Fusarium head blight ; scab ; resistance ; genetic resources ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four species of indigenous Japanese Agropyron, namely, Elymus humidus Osada (= Agropyron humidum), E. tsukushiensis Honda var. transiens (= A. tsukushiense), E. racemifer Tsvel. (= A. ciliare) and A. mayebaranum var. intermedium Hatusima were collected and evaluated for their resistance to Fusarium head blight (FHB, scab) after inoculation with a conidial suspension of Fusarium graminearum Schwabe at the flowering stage. The resistance to penetration of FHB and to fungal invasion of rachis internodes was evaluated in each accession in a comparison with six wheat cultivars. The results demonstrated that AG.91-35 of E. humidus and AG.91-24 of E. racemifer had higher resistance to penetration than the resistant wheat cultivars Nobeokabouzu-komugi and Sumai 3, respectively. All accessions of indigenous Japanese species of Agropyron examined, with the exception A. mayebaranum, had a statisticallysimilar resistance to invasion, which was the same as that of Nobeokabouzu-komugi or Sumai 3. In all accessions of E. humidus, with only one exception, no spread of the fungus from the infected spikelets to the rachis internodes was detected at all. The possible application of the strains of Agropyron as genetic resources for development of resistance to FHB in wheat is discussed.
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    URL: http://dx.doi.org/10.1023/A:1003078109694
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    Radiation interception and radiation use efficiency of near-isogenic wheat lines with different height (1997)
    Springer
    Euphytica 97 (1997), S. 201-208 
    Details
    ISSN: 1573-5060
    Keywords: dwarfing genes ; wheat ; radiation interception ; radiation use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three near isogenic lines of spring wheat grown to determine whether Rht dwarfing alleles alter radiation interception. A field study (involving two sowing dates in two growing seasons) with lines containing different allelic dosage of Rht1 and Rht2 (i.e. dwarf, DD; semi-dwarf, SD and tall SH), was conducted without water and nutritional deficiencies. Dwarfing genes did not modify the timing of occurrence of phenological events. Above-ground biomass at anthesis was reduced by 22% in the DD line in relation of the rest of the lines. However, at maturity accumulated biomass of the DD and SH lines were not significant different. Dwarfing genes increased the light attenuation coefficient (k, with values of 0.48, 0.62 and 0.78 for the SH, SD and the DD line respectively). A similar trend was followed to the leaf thickness (estimated by the specific leaf weight, SLW). Despite the differences observed among the lines, both in k and SLW values, they did not differ significantly in the proportion of incoming radiation intercepted by the canopy, nor in the cumulative intercepted radiation during the pre and post-anthesis periods. Radiation use efficiency (RUE) differed significantly among the lines. While RUE during pre-anthesis was the lowest in the DD line, from anthesis to maturity the lines with Rht alleles showed higher RUE values than the SH line. The low pre-anthesis RUE in the DD lines could be associated with (i) poor canopy architecture due to reductions in leaf sheath and internode lengths and/or (ii) reduced canopy CO2 exchange rate. Post-anthesis RUE was lower than that recorded pre-anthesis in all lines. But the magnitude of the reduction was inversely related to the doses of the Rht alleles. Post-anthesis RUE appeared to be closely and positively associated with the number of grains set per unit biomass at anthesis. This relationship suggests a regulatory effect of the sink size on the efficiency of the crop to convert radiation into biomass during this period.
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    URL: http://dx.doi.org/10.1023/A:1003061706059
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    Response of wheat genotypes to sowing date and boron fertilization aimed at controlling sterility in a rice-wheat rotation in Nepal (1997)
    Springer
    Plant and soil 188 (1997), S. 249-256 
    Details
    ISSN: 1573-5036
    Keywords: boron ; rice-wheat rotation ; sterility ; time of sowing ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spikelet sterility in wheat (Triticum aestivum L.) is emerging as a production threat in different parts of Nepal. This study was aimed at determining the effects of sowing date and boron application in controlling spikelet sterility in four different genotypes of spring wheat in a rice-wheat system in the western hills of Nepal. Four genotypes of known different responses to boron were planted on 21 November, 6 December and 21 December, 1994 with or without boron application at 1 kg B ha-1 (i.e. 9 kg borax ha-1) on a soil that was known to be deficient in boron. The effect of sowing date was significant for the phenology, yield components, percentage sterility and grain yield. Sterility was significantly increased in the crop planted on 21 December, which had also the lowest 1000 seed weight and grain yield; there was an almost 50% grain yield reduction compared to the crop planted on 21 November. Terminal moisture stress (i.e. lack of moisture during the later part of the development) was observed in the late sown crop which also amplified the extent of sterility associated with boron deficiency. Genotypes differed in response to sowing dates and boron treatment for all of the phenological events measured, yield components, grain yield and percentage sterility. SW-41 and BL-1022 had significantly higher sterility at all sowing dates. BL-1249 showed a consistently lower% sterility over all sowing dates and boron treatments. The addition of boron significantly increased the number of grains set per spike thereby decreasing the total sterility in boron responsive genotypes SW-41 and BL-1022 while those not susceptible did not respond. The boron concentration in the flag leaf at anthesis was increased in treatments with added B in the soil but genotypes did not differ in boron concentration for any soil treatment.
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    URL: http://dx.doi.org/10.1023/A:1004202725382
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    Hydroponic culture of the mycorrhizal fungus Glomus mosseae with Linum usitatissimum L., Sorghum bicolor L. and Triticum aestivum L. (1997)
    Springer
    Plant and soil 196 (1997), S. 143-149 
    Details
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhiza ; hydroponics ; linseed ; sorghum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Linum usitatissimum, Sorghum bicolor and Triticum aestivum plants were further colonised by the arbuscular mycorrhizal fungus, Glomus mosseae, during a four week period of hydroponic culture after a pre-culture period of three weeks with the fungus in perlite substrate. The viability of mycorrhizal colonisation of T. aestivum was indicated by an initial experiment where G. mosseae from mycorrhizal plants colonised non-mycorrhizal plants when the plants were grown together in the same hydroponic container using modified Long Ashton nutrient solution. Intermittant aeration of the plant roots (2 h periods, four times per day) provided a compromise between adequate aeration and minimal disturbance of the fungus. In a second experiment, two nutrient media, modified Long Ashton and modified Knop plus Hoagland medium were compared for culturing G. mosseae on T. aestivum. A significantly higher root dry weight was found for the mycorrhizal versus the non-mycorrhizal wheat plants in modified Long Ashton nutrient medium, which contained 10 µM P and an organic buffer. Modified Knop plus Hoagland nutrient medium contained a high P concentration (0.9 mM) and did not produce viable cultures of mycorrhizal colonisation. In a third experiment, modified Long Ashton medium was used for hydroponic culture of mycorrhizal L. usitatissimum, S. bicolor and T. aestivum. The root colonisation percentages for T. aestivum (73%), S. bicolor (36%) and L. usitatissimum (65%) were within the range of colonisation rates obtained with solid substrate culture in perlite. Viability of the mycorrhizal structures in hydroponic culture was assessed by monitoring activity of fungal succinate dehydrogenase and found to be similar to cultures in perlite. No difference in the P concentration of mycorrhizal and non-mycorrhizal plants was observed, possibly owing to the lack of diffusion limits for P in hydroponic solution. This report describes a system for the viable culture of G. mosseae with different plant species where a high mycorrhizal colonisation rate was produced under conditions of a short culture period using intermittent aeration, a low concentration of P supply and an organic buffer.
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    Isolation, identification and characterization of disomic and translocated barley chromosome addition lines of common wheat (1997)
    Springer
    Euphytica 96 (1997), S. 289-296 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Hordeum vulgare ; wheat ; barley ; chromosome addition lines ; translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two disomic barley chromosome addition lines and five translocated chromosome addition lines of common wheat cultivar Shinchunaga were isolated. They were derived from a hybrid plant between Shinchunaga and cultivated barley Nyugoruden (New Golden) by backcrossing with wheat and self pollination. Barley chromosomes added to chromosome arms involved in the translocated chromosomes were identified by C-banding method and by crossing these lines with Chinese Spring/Betzes addition lines. Two disomic addition lines were identified to have chromosome 6 and 7 of barley, respectively. Two of the five translocated chromosome addition lines were clarified to have same chromosome constitution, 42 wheat chromosomes and a pair of translocated chromosomes constituted with a long arm of chromosome 5B of wheat and a short arm of chromosome 7 of barley. The other three lines could not be identified due to chromosome rearrangement. Performances of these seven lines on agronomic characters were examined. Addition of barley chromosome 7 induced early heading, and chromosome 6 showed lated heading. Almost all of the lines except that of chromosome 6 showed short culm length and all showed reduced number of tillers, spikelets and grains per ear, and low seed fertility. These lines would be useful for genetic analyses in wheat and barley and for induction of useful genes of barley into wheat.
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    URL: http://dx.doi.org/10.1023/A:1003081619338
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    Structuring genotype × environment interaction for quality traits in bread wheat, in two multi-location series of trials (1997)
    Springer
    Euphytica 97 (1997), S. 53-66 
    Details
    ISSN: 1573-5060
    Keywords: clustering procedure ; genotype × environment interaction ; multi-trait analysis ; quality ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Structure of genotype × environment interaction was studied in two series of trials for three quality traits in bread wheat. Two kinds of environments were present in each series of trials: macro-environments defined as locations or location × year combinations and micro-environments induced by diversified cultural practices within each site. For each trait, a simultaneous clustering procedure was used to identify groups of environments which were homogeneous for interaction. An optimised series of trials was proposed from the clusters obtained. The cultural practice based on nitrogen fertilisation seemed to better diversify environments for interaction than use of fungicide, when all quality traits were considered. Determining an optimised series of trials simultaneously for the three traits led to keeping more environments than when one trait was considered. Suggestions for establishing a series of trials for a multi-trait analysis were proposed.
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    URL: http://dx.doi.org/10.1023/A:1003048720423
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    Genetic suppression of the cereal rye-derived gene Pm8 in wheat (1997)
    Springer
    Euphytica 93 (1997), S. 353-360 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Secale cereale ; powdery mildew ; Erysiphe graminis f.sp. tritici ; resistance ; suppression ; segregation distortion ; wheat ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The location of wheat gene Pm8 for resistance to powdery mildew in the 1RS chromosome segment derived from cereal rye cultivar Petkus was confirmed. There was reduced transmission of the 1BL.1RS chromosome relative to its wheat homologue in four of the five crosses examined. Pm8 was not expressed in some wheat cultivars and selections which possessed the 1RS chromosome identified by the presence of relevant genes for resistance to the three rusts, due to the presence of a dominant suppressor gene(s) in the wheat genome. The frequency of suppression in 1BL.1RS wheats from Mexico was significantly higher than in a group of wheats (both local and exotic) introduced from China and was probably much higher than in European wheats.
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    Sources of resistance to head scab in Triticum (1997)
    Springer
    Euphytica 94 (1997), S. 31-36 
    Details
    ISSN: 1573-5060
    Keywords: Triticum ; Fusarium graminearum ; resistance ; head scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Multi-floret and single-floret injection inoculation methods were used to test 1076 accessions of Triticum for resistance to initial infection and resistance to pathogen spread within spike tissue respectively. The data obtained between years or inoculation dates were comparatively similar and were little influenced by climatic factors, especially humid condition. All the tested materials uniformly exhibited susceptibility to initial infection, but there were great differences in resistance to spread among species or cultivars. The diploid and tetraploid wheats were severely susceptible. Only 30 genotypes of common wheat of T. aestivum concv. vulgare showed high resistance to spread. The highest frequency of high spread resistance existed in these landraces which were distributed in Zhejiang, Hubei, Hunan, Jiangsu provinces and Shanghai City, Guizhou, Sichuan and Yunnan provinces ranked second. The majority of wheat landraces from Hebei, Shanxi, Shaanxi Province and Beijing City showed susceptibility or high susceptibility. No resistance was found in Tibetian wheats.
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    Resistance to stripe rust in durum wheats, A-genome diploids, and their amphiploids (1997)
    Springer
    Euphytica 94 (1997), S. 279-286 
    Details
    ISSN: 1573-5060
    Keywords: Triticum turgidum ; T. monococcum ; T. boeoticum ; T. urartu ; wheat ; Puccinia striiformis ; stripe rust ; rust resistance ; amphiploid ; suppressors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Stripe rust (caused by Puccinia striiformis Westend.) is a wheat disease of worldwide importance. Seedlings of 75 accessions of Triticum boeoticum, 12 of T. monococcum, 16 of T. urartu, 230 of durum wheat (T. turgidum L. var. durum), and 128 amphiploids (genome AAAABB) involving the crosses of the three diploid species (AA) with T. turgidum (AABB) were evaluated in the greenhouse for their reaction to P. striiformis race 14E14. Durum wheats and the amphiploids were also evaluated at two field locations in Mexico with the same race for their adult plant response. Resistant seedling reactions (infection types: 0-3 on a 0-9 scale) were seen for 10 (13%) accessions of T. boeticum, 19 (8%) accessions of T. turgidum and 32 (25%) amphiploids. The remaining accessions were either moderately resistant (ITs 4-6) or susceptible (ITs 7-9). The three amphiploids derived from the crosses of seedling resistant T. boeoticum and T. turgidum, were resistant as seedlings. Among the 51 amphiploids involving one resistant parent, 29 were resistant and the remaining 22 displayed intermediate to susceptible reactions. Suppressors for resistance were common in the A and AB genomes and suppression was resistance gene specific. Forty-five (20%) durums showed adequate field resistance (relative AUDPC 〈10% of the susceptible check ‘Morocco’). These included the 19 seedling resistant durums. Presence of genes involved in adult plant resistance was evident, because 26 of the remaining adult plant resistant durums had displayed intermediate-susceptible seedling reactions. Though the seedling reactions of the amphiploids varied from low to high, all involving the adult plant resistant durums possessed adequate field resistance. The resistant, newly produced, AAAABB amphiploids are useful genetic resources for stripe rust resistance which could be transferred to the cultivated T. turgidum.
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    URL: http://dx.doi.org/10.1023/A:1002979706378
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    Evaluation and variation in response to infection with Puccinia striiformis and Puccinia recondita of local wheat landraces (1997)
    Springer
    Euphytica 94 (1997), S. 287-293 
    Details
    ISSN: 1573-5060
    Keywords: Israel ; landraces ; Puccinia recondita ; Puccinia striiformis ; disease resistance ; wheat ; Triticum spp. ; leaf rust ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The purpose of this study was to identify the species of local landraces of wheat (Triticum spp.), held in the Israel Gene Bank, to evaluate them for basic characters and to assess their response to infection by two rust fungi under artificial inoculation conditions. One-hundred-thirty one seed samples were collected from local or Beduin farmers during 1978–1981 throughout the Galilee, Mt. Gilboa. Judean Desert and the south Negev. The samples were collected and stored in the Israel Gene Bank without any characterization or evaluation. Each accession was planted in a 1 m row at Bet Dagan and grown under favorable conditions for plant growth and rust development. Determination of the species, data of plant height, days to heading and reaction of the landraces to artificial inoculation with a composite inoculum of Puccinia recondita and P. striiformis were collected from each row. A small part of the landraces collection consisted of mixed populations of T. durum and T. aestivum plants, where one of the two species was predominant. One-hundred-fourteen and 17 accessions from this collection represented, respectively, Triticum durum and T. aestivum Israel landraces. Large variations were found for all the characters examined. Of the total accessions, 6.5% (8 accessions) and 32% (42 accessions) were resistant, respectively, to yellow- and leaf-rust. It was concluded that the diversified populations of the local landraces of wheat can be used as a source not only for genes affecting basic characters such as plant height and heading date, but also for resistance to leaf rust and yellow rust.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002983824125
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    Further manipulation by centric misdivision of the 1RS.1BL translocation in wheat (1997)
    Springer
    Euphytica 94 (1997), S. 257-261 
    Details
    ISSN: 1573-5060
    Keywords: centric breakage and fusion ; translocation ; substitution ; wheat ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Complete chromosomes 1R and 1B were reconstructed in wheat from the centric wheat-rye translocation 1RS.1BL. Three substitutions: 1R(1A), 1R(1B), 1R(1D), and three new centric translocations: 1RS.1AL, 1RS.1BL, 1RS.1DL were produced from the reconstructed chromosome 1R. Each one of these has the same rye chromosome arm 1RS which was present in the original translocation 1RS.1BL of ‘Kavkaz’ wheat. Reconstructed chromosome 1B and a normal chromosome 1R were used to produce a new 1RS.1BL translocation. This translocation has the long arm from the original 1RS.1BL translocation of ‘Kavkaz’, but a different 1RS arm. The third generation centric translocations were mitotically stable and were normally transmitted to progeny. Misdivision frequency of the reconstructed chromosomes 1R did not change relative to normal 1R, whereas the misdivision frequency of the two reconstructed chromosomes 1B tested was significantly higher relative to normal 1B. These experiments demonstrate that repeated cycles of centric breakage and fusion do not impair the function of centromeres in wheat and rye but may change chromosome's susceptibility to misdivision.
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    Variation in sterility among wheat (Triticum aestivum L.) genotypes in response to boron deficiency in Nepal (1997)
    Springer
    Euphytica 95 (1997), S. 21-26 
    Details
    ISSN: 1573-5060
    Keywords: Boron ; genotypic variability ; sterility ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two field experiments were carried out at the Khairanitar Farm, Nepal during the winter season of 1993 to investigate whether wheat genotypes differ in their sterility response to low soil boron and whether boron added to soil can correct sterility. A nursery consisting of 41 diverse genotypes from Nepal, India, Bangladesh, Thailand, China, Pakistan and CIMMYT/Mexico were evaluated in a plot where severe sterility had been observed in previous years. The second experiment involved six genotypes of known sterility responses to boron grown with or without an application of boron at 1 kg ha-1. Large differences were observed among genotypes in terms of number of grain set per ear (range 0.5 to 30 ) and sterility (5.5% to 97.5%). About one third of all the genotypes had 〈25% sterility and 16 of them suffered 〉75% sterility. The genotypes Fang-60, Sonalika, De Mai 6-22, BUC/FLK/MYNA/VL and HDW-234 were highly tolerant to boron deficiency with 〈10% sterility. In sharp contrast, genotypes BOW/BUT, SERI/THB, Glennson, SW-41, Yunmai-33 and UHU were highly susceptible and set virtually no grains in the boron deficient plots. Strong responses to boron application were observed and genotypic variations were evident. Boron at 1 kg ha-1significantly reduced the number of late ears, increased number of grains per ear and grain yield in boron-responsive genotypes. Tolerant genotypes were not influenced by added boron. Given these striking genetic differences, progress in breeding wheat for B-deficient soils in Nepal should be worthwhile.
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    URL: http://dx.doi.org/10.1023/A:1002926117203
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    Comparison of the effects of nitrate and ammonium forms of nitrogen on auxin content in roots and the growth of plants under different temperature conditions (1997)
    Springer
    Plant growth regulation 23 (1997), S. 207-208 
    Details
    ISSN: 1573-5087
    Keywords: auxin ; immunoassay ; nitrogen nutrition ; root growth ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Average root length, root/shoot ratio and auxin content in roots were higher in plants supplied with nitrate rather than ammonium and grown at 18, 21, 24°C. The effects on root length were most pronounced at the highest temperatures (21 and 24°C); and the warmer the temperature, the earlier appearance of the differences in growth rate between NO3- and NH4-fed plants. A sharp acceleration of root growth was characteristic of NO3-fed plants grown at 21 and 24°C and was associated with a temporary increase in auxin concentration measured by immunoassay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005990725068
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    Relative efficacy of paclobutrazol, propiconazole and tetraconazole as stress protectants in wheat seedlings (1997)
    Springer
    Plant growth regulation 21 (1997), S. 169-175 
    Details
    ISSN: 1573-5087
    Keywords: chilling ; drought ; heat ; paclobutrazol ; paraquat ; propiconazole ; protection ; tetraconazole ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The stress protective effects of triazoles including paclobutrazol, a plant growth regulator, and two fungicides, propiconazole and tetraconazole, are compared. Wheat (Triticum aestivum L. cv Katepwa) seeds were imbibed for 18 h in distilled water (Ck) or in aqueous solutions of each triazole (50 mg L-1). Seeds were then air dried, planted in sectioned plastic flats and grown in a greenhouse. After 10 days, one set of seedlings were allowed to continue growing under optimal conditions while additional sets were exposed to various stresses including high temperatures, drought and spray with the herbicide paraquat. Compared to wheat leaves from plants grown under optimal conditions, heat stress decreased shoot fresh weight, fluorescence values and chlorophyll levels. It also increased ion leakage. All symptoms of damage were alleviated by the triazoles, with paclobutrazol being the most potent. Similar trends were found under acute drought conditions, where seedlings treated with paclobutrazol had the highest percent survival and the most shoot regrowth upon rewatering. Paclobutrazol was also the best triazole in protecting wheat leaves from damage caused by paraquat, a free radical generator. It is concluded that while all the triazoles have the potential to be stress protectants, paclobutrazol was consistently the most effective.
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    URL: http://dx.doi.org/10.1023/A:1005804717016
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    Wheat and Barley Inhibitors Active Towards α-Amylase and Trypsin-like Activities from Spodoptera frugiperda (1997)
    Springer
    Journal of chemical ecology 23 (1997), S. 1729-1741 
    Details
    ISSN: 1573-1561
    Keywords: α-Amylase inhibitors ; trypsin inhibitors ; cereal endosperm ; wheat ; barley ; Spodoptera frugiperda ; digestive enzymes ; midgut proteases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The α-amylase activity was determined throughout the larval development of Spodoptera frugiperda. Maximal activities with optimal pH in the range 8.5–9.5 were found in last instars. Protein preparations enriched in heterotetrameric inhibitors from wheat flour were active towards gut amylases from last instars, while those corresponding to homodimeric and monomeric inhibitors showed low inhibition levels. These results were further supported by testing purified members of each inhibitor type and by analyzing the effects of the inhibitors on the amylase isoenzyme pattern from native PAGE. High levels of trypsin-like activity were also found in gut extracts from last instars. Different genetic variants of the major barley trypsin inhibitor were active against this gut enzyme. None of the other larval digestive protease activities (chymotrypsin-like, elastase-like, leucine aminopeptidase-like, and carboxypeptidase A and B-like) were inhibited, indicating that the barley inhibitor is specific towards trypsin-like enzymes.
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    Semiochemicals Mediating Spacing Behavior of Bird Cherry-Oat Aphid, Rhopalosiphum padi Feeding on Cereals (1997)
    Springer
    Journal of chemical ecology 23 (1997), S. 2599-2607 
    Details
    ISSN: 1573-1561
    Keywords: Spacing pheromones ; olfactometry ; aphids ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Olfactometry using an apterous individual of Rhopalosiphum padi (L.) showed an arresting effect by volatiles from a wheat seedling and a repellent effect by volatiles from a wheat seedling infested with aphids at a high population density (ca. 9 aphids/cm2). Four compounds, 6-methyl-5-hepten-2-one, (−)- and (+)-6-methyl-5-hepten-2-ol, and 2-tridecanone, were identified by GC-MS in air entrainments from the wheat seedlings with high aphid density but not from the wheat seedlings alone. The mixture of the four compounds in the natural proportion counteracted the attractivity of the volatiles from the intact uninfested wheat seedling. The likely role of these compounds in the spacing behavior of this aphid species, when present in high densities on wheat, is discussed.
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    URL: http://dx.doi.org/10.1023/B:JOEC.0000006669.34845.0d
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    Environmental Effects on the Accumulation of Hydroxamic Acids in Wheat Seedlings: The Importance of Plant Growth Rate (1997)
    Springer
    Journal of chemical ecology 23 (1997), S. 543-551 
    Details
    ISSN: 1573-1561
    Keywords: Temperature ; photoperiod ; hydroxamic acids ; growth ; defense ; secondary metabolites ; wheat ; Gramineae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effects of temperature and photoperiod on accumulation of hydroxamic acids (Hx) in wheat (Triticum aestivum L.) seedlings were evaluated under laboratory conditions. Hx concentrations were significantly higher at higher temperatures. No such clear trend was found for the photoperiod effect. The significant effect of temperature and photoperiod on growth rate of seedlings and the significant positive correlation between growth rate prior to analysis and levels of Hx, suggested that environmental effects on Hx accumulation were at least partially mediated through their effect on plant growth rate. After uncoupling the effect of environmental conditions from the effect of plant growth rate by statistical means the effect of temperature on Hx was no longer significant. Therefore, temperature effect was fully mediated by plant growth rate. Implications of the patterns found are discussed in issues of plant-defense general theories.
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    URL: http://dx.doi.org/10.1023/B:JOEC.0000006376.92876.14
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    Modelling Crop-Climate Interactions in Spain: Vulnerability and Adaptation of Different Agricultural Systems to Climate Change (1997)
    Springer
    Mitigation and adaptation strategies for global change 1 (1997), S. 273-288 
    Details
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Spain ; wheat ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This study evaluates the theoretical impact of climate change on yields and water use of two crops with different responses to increased CO2 and which represent contrasting agricultural systems in Spain. In all cases the simulated effects of a CO2-induced climate change depended on the counteracting effects between higher daily ET rates, shortening of crop growth duration and changes in precipitation patterns as well as the simulated effects of CO2 on the water use efficiency of the crops. For summer irrigated crops such as maize, the yield reductions and the exacerbated problems of irrigation water availability simulated with climate change may force the crop out of production in some regions. For winter dryland crops such as wheat, productivity increased significantly in some regions, suggesting a northward shift of area suitable for wheat production in future climates. The study considered strategies for improving the efficiency of water use based on the optimization of crop management decisions in a CO2-driven warmer climate.
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    URL: http://dx.doi.org/10.1023/B:MITI.0000018139.42373.f1
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    Modelling crop-climate interactions in Spain: Vulnerability and adaptation of different agricultural systems to climate change (1997)
    Springer
    Mitigation and adaptation strategies for global change 1 (1997), S. 273-288 
    Details
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Spain ; wheat ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This study evaluates the theoretical impact of climate change on yields and water use of two crops with different responses to increased CO2 and which represent contrasting agricultural systems in Spain. In all cases the simulated effects of a CO2-induced climate change depended on the counteracting effects between higher daily ET rates, shortening of crop growth duration and changes in precipitation patterns as well as the simulated effects of CO2 on the water use efficiency of the crops. For summer irrigated crops such as maize, the yield reductions and the exacerbated problems of irrigation water availability simulated with climate change may force the crop out of production in some regions. For winter dryland crops such as wheat, productivity increased significantly in some regions, suggesting a northward shift of area suitable for wheat production in future climates. The study considered strategies for improving the efficiency of water use based on the optimization of crop management decisions in a CO2-driven warmer climate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00517807
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    Trees on farms in Bangladesh: 5. Growth of top- and root-pruned trees in wetland rice fields and yields of understory crops (1997)
    Springer
    Agroforestry systems 39 (1997), S. 101-115 
    Details
    ISSN: 1572-9680
    Keywords: canopy pruning ; cereals ; pulses ; root pruning ; tree-crop competition ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Growth of trees and seasonal yields of understory crops were measured over a 5-year period for 4 crops grown under 17 tree species at 8 × 8 m spacing in wetland rice fields. All tree species grew well in rice fields, at rates comparable to their growth in forest plantations. Top and root pruning reduced average tree girths by up to 19% and average tree volumes by up to 41%, depending on intensity of pruning. The crops monitored were Oryza sativa, Triticum aestivum, Corchorus olotorius, and Lens culinaris. Crop yields under the trees averaged 93% of the corresponding yields outside the tree canopy. The most important factor affecting the yields of undercrops was tree size (height and/or girth). Differences among tree species and the interaction with species of undercrops were not significant after controlling for tree size. Pruning of roots and branches significantly improved crop yields under trees by amounts proportional to the intensity of root or top pruning.
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    URL: http://dx.doi.org/10.1023/A:1005999225770
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    Genetic variability at the Glu-1 loci in old and modern wheats (triticum aestivum L.) cultivated in Slovakia (1997)
    Springer
    Genetic resources and crop evolution 44 (1997), S. 301-306 
    Details
    ISSN: 1573-5109
    Keywords: genetic variability ; high molecular weight glutenin subunits ; storage proteins ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The high molecular weight glutenin subunits (HMW-GS) composition at the Glu-1 complex loci, in 23 old original wheat genotypes cultivated in Slovakia several decades ago and 32 modern Slovak and Czech wheat cultivars growed in Slovakia at present were studied by SDS-PAGE. Some of the HMW-GS – subunit pairs 3+12, 17+18, and subunit 20, present in old “historical” wheats were missing in modern cultivars utilized in Slovakia nowadays. There were observed 15 different HMW-GS encoded by 11 alleles or allelic pairs in old genotypes. Lower number of different HMW-GS and competent alleles were observed in a set of modern wheat cultivars – 11 different HMW glutenin subunits encoded by 8 alleles or allelic pairs. The same number of different HMW-GS patterns was revealed in both sets of wheats. From the point of view of genetic variability, it could be concluded that long-term effort of breeders and decreasing of cultivation of landraces and old cultivars are associated with the loss of several HMW-GS alleles and decreasing of genetic variability of wheats. Molecular characterization can reveal broad allelic variability of old wheat genotypes and landraces. Their maintenance in genetic resource collections can prevent losses of these interesting genes.
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    URL: http://dx.doi.org/10.1023/A:1008687722002
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    Evaluation of Roegneria for resistance to head scab caused by Fusarium graminearum Schwabe (1997)
    Springer
    Genetic resources and crop evolution 44 (1997), S. 211-215 
    Details
    ISSN: 1573-5109
    Keywords: Fusarium graminearum ; resistance ; Roegneria ; scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract 71 accessions from 13 Roegneria species were evaluated for resistance to wheat scab. The results showed that 31 of the 71 accessions possessed high resistance (HR) to initial infection and high resistance (HR) to pathogen spread. The disease spread from inoculated floret to head rachis was not observed, and the incidence of head infection were lower under natural than under artificial inoculation conditions. The intergeneric hybrid F1 of Roegneria tsukushiensis (Honda) B.R. Lu, Yen et J.L. Yang. var. transiens (Hack.) B.R. Lu, Yen et J.L. Yang × Hordeum vulgare Linn. exhibited resistance (R) to pathogen invasion and high resistance (HR) to spread. Therefore, Roegneria could be used as scab resistant resources in cereal breeding. In addition, the relatioship between its resistance and ecological environments where Roegneria was distributed was discussed in this paper.
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    URL: http://dx.doi.org/10.1023/A:1008643617661
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    Changes in wheat seed storage protein fingerprint due to soil mineral content (1997)
    Springer
    Euphytica 95 (1997), S. 209-219 
    Details
    ISSN: 1573-5060
    Keywords: gene expression ; gluten ; glutenin ; protein fingerprint ; Triticum turgidum ; var. dicoccoides ; wheat ; Triticum aestivum ; Triticum durum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat seed storage protein fingerprint is used to determine the gluten protein pattern in studies aimed at improving flour quality. Wild wheat with high seed protein content is used extensively in wheat breeding programs. Although the wild wheat growth and protein content may be influenced by environmental conditions, the gluten-protein pattern is generally considered as indicative of a genotype, without the superimposition of environmental influences. The effects of soil type, habitat, and deficiencies of N, P, K and S on seed storage protein composition were examined in nine accessions of wild wheat (Triticum turgidum var. dicoccoides) and three varieties (two T. aestivum and one T. durum). Soil from ten natural habitats of the wild wheat that had not previously received any fertilizers or manures was sampled and used to grow wheat in a greenhouse. Seed storage protein composition was characterized by SDS-PAGE. Although deficiencies in soil nutrient caused variations in the seed storage proteins, the genotype was the main factor determining the seed storage protein composition. Seed storage protein composition of genotypes varied when grown under different mineral nutrient conditions. Only one genotype was stable showing almost identical protein patterns under all growing conditions studied without any qualitative change in fingerprint pattern. In the other genotypes, as well as the cultivars, the seed storage protein was affected at least to some extent by the soil. The ‘soil effect’ is summarized in terms of three main quantitative changes in the seeds: 1 – the relative amounts of the high-molecular-weight proteins; 2 – the relative amounts of proteins in the range of 45 and 65 kD; 3 – the percentage distribution of the HMW glutenin and other groups of seed storage proteins. The soild induced also qualitative differences in the composition of seed storage proteins, mostly in those of 45–65 kD. These differences were observed whenever a deficiency of S, N, P, K or Mg was identified. Therefore, in breeding programs that use seed storage protein fingerprints of wild wheat germplasms should be exercise caution when the germplasms selected from wild habitats.
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    URL: http://dx.doi.org/10.1023/A:1002908024652
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    Boron deficiency induced male sterility in wheat (Triticum aestivum L.) and implications for plant breeding (1997)
    Springer
    Euphytica 96 (1997), S. 257-262 
    Details
    ISSN: 1573-5060
    Keywords: boron deficiency ; fertility selective media ; hybridization ; male sterility ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Boron (B) deficiency causes grain set in wheat to fail. A wide range of genotypic variation in the response to low B has been observed. Genotypes were screened in low B in soil and sand culture, and classified into five groups, namely, very sensitive, sensitive, moderately sensitive, moderately tolerant and tolerant. At very low levels of B, the very sensitive to sensitive genotypes were completely male sterile and set only a few or no grain, while the tolerant genotypes set grain normally. Natural outcrossing was detected in these male sterile plants when a tolerant genotype was growing nearby. Grain set by cross fertilisation was markedly enhanced by a B application directly on the ear of the male sterile plants. Three practical implications are suggested. Firstly, genotypes that are tolerant to low B can provide a solution for grain set failure caused by B deficiency. Secondly, the potential for outcrossing in male sterile B deficient wheat has to be taken into account in the maintenance of pure lines in low B soils even though wheat is normally self pollinated. Thirdly, a simple and novel method for hybridization is suggested, in which B deficiency is used as fertility selective medium and male sterile female parents and fertile male parents are provided by genotypic variation in the response to low B.
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    URL: http://dx.doi.org/10.1023/A:1003093532561
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    Wide hybridization between wheat (Triticum L.) and lymegrass (Leymus Hochst.) (1997)
    Springer
    Euphytica 93 (1997), S. 293-300 
    Details
    ISSN: 1573-5060
    Keywords: lymegrass ; Triticum ; wheat ; wide hybrids ; cytogenetics ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A total of 240 F1 hybrids was made beween wheat (Triticum aestivum L. em. Thell. (2n = 6x = 42) and T. carthlicum Nevski (2n = 4x = 28)) and perennial lymegrass (North European Leymus arenarius (L.) Hochst. (2n = 8x = 56) and North American L. mollis (Trin.) Pilger (2n = 4x = 28)). The wide crosses yielded embryos in 20% of caryopses and 96% of the embryos developed into normal hybrid plants. The hybrids were vegetatively vigorous, with evidence of the Leymus rhizomatous habit. Those deriving from L. arenarius survived overwintering in Iceland, but the hybrids L. mollis did not, whereas in a milder environment, both showed perenniality. Cytogenetic analysis of root tip cells before the plants were treated with colchicine showed that 21 out of 28 hybrids investigated had chromosome mosaics, with a population of both amphihaploid and amphidiploid cells. This spontaneous doubling of somatic chromosomes occurred in all cross combinations, with the highest average frequency of diploid cells (28%) in T. carthlicum × L. arenarius crosses. A few selfed seeds have been obtained from a T. aestivum × L. arenarius hybrid. All the hybrids were treated twice with colchicine, but the treatment appeared to have little or no effect on the frequency of chromosome doubling in the hybrids deriving from T. aestivum. The frequency of diploid cells, however, increased significantly (e.g. to 80%) in the hybrids deriving from the T. carthlicum parent. Genomic in situ hybridization confirmed the hybridity of the plants and showed that the hybrids were amphiploids containing genomes of both wheat and lymegrass. In situ hybridization using ribosomal DNA probe differentiated chromosomes of L. mollis, L. arenarius from those of wheat. The hybrids are being backcrossed with lymegrass pollen, aiming to domesticate the wild, perennial species.
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    Productivity in Great Plains acid soils of wheat genotypes selected for aluminium tolerance (1997)
    Springer
    Plant and soil 188 (1997), S. 101-106 
    Details
    ISSN: 1573-5036
    Keywords: acid soil tolerance ; Al tolerance ; selection ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil acidity in the Great Plains of the USA can reduce forage and grain yields of winter wheat, primarily by Al toxicity. Indigenous cultivars may vary in seedling tolerance to Al toxicity, but the benefit that Al tolerance provides to forage and grain production is not well documented in this region. Backcrossed-derived lines of ‘Chisholm’ and ‘Century’ were selected with an additional gene from ‘Atlas 66’ conferring Al tolerance in solution culture. Our objective was to determine the impact of this source of Al tolerance on forage production prior to the jointing stage and subsequent grain yield. Experiments were conducted at several locations on non-limed (pH=4.5–4.7) and limed soils (pH=5.2-6.1) in Oklahoma. Two cultivars (‘TAM 105’, susceptible; ‘2180’, tolerant) with extreme differences in Al tolerance were used as controls . In limed conditions, forage and grain production did not differ between Al-tolerant and -susceptible genotypes, indicating a neutral effect of the Atlas 66 gene in the absence of Al toxicity. Despite visual differences in early-season plant vigor in non-limed acid soil, the Al-tolerant selections did not yield greater season-long forage than their susceptible parents. At sites where Al saturation in the non-limed soil exceeded 30%, spike production at maturity was nearly doubled in the Century background by the addition of Al tolerance, but final grain yield was not significantly improved. In the Chisholm background, grain yield was improved 50 to 74% by Al tolerance. The magnitude of the agronomic benefit of Al tolerance was highly influenced by the edaphic environment and genetic background. Acid soils of the Great Plains appear highly variable in Al toxicity; hence, consideration of the target environment is essential to predict the potential impact of Al tolerance selected in solution culture.
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    URL: http://dx.doi.org/10.1023/A:1004268325067
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    Differential response of rye, triticale, bread and durum wheats to zinc deficiency in calcareous soils (1997)
    Springer
    Plant and soil 188 (1997), S. 1-10 
    Details
    ISSN: 1573-5036
    Keywords: genotypic differences ; rye ; triticale ; wheat ; zinc deficiency ; zinc efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field and greenhouse experiments were carried out to study the response of rye (Secale cereale L. cv. Aslim), triticale (× Triticosecale Wittmark. cv. Presto), two bread wheats (Triticum aestivum L, cvs. Bezostaja-1 and Atay-85) and two durum wheats (Triticum durum L. cvs. Kunduru-1149 and C-1252) to zinc (Zn) deficiency and Zn fertilization in severely Zn-deficient calcareus soils (DTPA-Zn=0.09 mg kg-1 soil). The first visible symptom of Zn deficiency was a reduction in shoot elongation followed by the appearance of whitish-brown necrotic patches on the leaf blades. These symptoms were either absent or only slight in rye and triticale, but occurred more rapidly and severely in wheats, particularly in durum wheats. The same was true for the decrease in shoot dry matter production and grain yield. For example, in field experiments at the milk stage, decreases in shoot dry matter production due to Zn deficiency were absent in rye, and were on average 5% in triticale, 34% in bread wheats and 70%, in durum wheats. Zinc fertilization had no effect on grain yield in rye but enhanced grain yield of the other cereals. Zinc efficiency of cereals, expressed as the ratio of yield (shoot dry matter or grain) produced under Zn deficiency compared to Zn fertilization were, on average, 99% for rye, 74% for triticale, 59% for bread wheats and 25% for durum wheats. These distinct differences among and within the cereal species in susceptibility to Zn deficiency were closely related to the total amount (content) of Zn per shoot, but not with the Zn concentrations in shoot dry matter. For example, the most Zn-efficient rye and the Zn-inefficient durum wheat cultivar C-1252 did not differ in shoot Zn concentration under Zn deficiency, but the total amount of Zn per whole shoot was approximately 6-fold higher in rye than the durum wheat. When Zn was applied, rye and triticale accumulated markedly more Zn both per whole shoot and per unit shoot dry matter in comparison to wheats. The results demonstrate an exceptionally high Zn efficiency of rye and show that among the cereals studied Zn efficiency declines in the order rye〉triticale〉bread wheat〉durum wheat. The differences in expression of Zn efficiency are possibly related to a greater capacity of efficient genotypes to acquire Zn from the soil compared to inefficient genotypes.
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    Role of rye chromosomes in improvement of zinc efficiency in wheat and triticale (1997)
    Springer
    Plant and soil 196 (1997), S. 249-253 
    Details
    ISSN: 1573-5036
    Keywords: addition lines ; rye ; triticale ; wheat ; zinc deficiency ; zinc genetic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using the disomic wheat-rye addition lines (Triticum aestivum L., cv. Holdfast-Secale cereale L., cv. King-II) and an octoploid triticale line (xTriticosecale Wittmark L. "PlutoxFakon") as well as the respective wheat and rye parents, greenhouse experiments were carried out to study the role of rye chromosomes on the severity of Zn deficiency symptoms, shoot dry matter production, Zn efficiency, shoot Zn concentration and Zn content. Plants were grown in a Zn-deficient calcareous soil with (10 mg Zn kg-1 soil) and without Zn supply. Zinc efficiency was calculated as the ratio of dry weight produced under Zn deficiency to the dry weight produced under Zn fertilization. In the experiments with addition lines, visual Zn deficiency symptoms were slight in the rye cultivar King-II, but were severe in the wheat cultivar Holdfast. The addition of rye chromosomes, particularly 1R, 2R and 7R, into Holdfast reduced the severity of deficiency symptoms. Holdfast showed higher decreases in shoot dry matter production by Zn deficiency and thus had a low Zn efficiency (53 %), while King-II was less affected by Zn deficiency and had a higher Zn efficiency (89 %). With the exception of the 3R line, all addition lines had higher Zn efficiency than their wheat parent: the 1R line had the highest Zn efficiency (80 %). In the experiment with the triticale cultivar and its parents, rye cv. Pluto and wheat cv. Fakon, Zn deficiency symptoms were absent in Pluto, slight in triticale and very severe in Fakon. Zinc efficiency was 88 % for Pluto, 73 % for triticale and 64% for Fakon. Such differences in Zn efficiency were better related to the total amount of Zn per shoot than to the amount of Zn per unit dry weight of shoot. Only in the rye cultivars, Zn efficiency was closely related with Zn concentration. Triticale was more similar to rye than wheat regarding Zn concentration and Zn accumulation per shoot under both Zn-deficient and Zn-sufficient conditions. The results presented in this study show that rye has an exceptionally high Zn efficiency, and the rye chromosomes, particularly 1R and 7R carry the genes controlling Zn efficiency. To our knowledge, the result with triticale and its rye parents is the first report showing that the genes controlling Zn efficiency in rye are transferable into wheat and can be used for development of new wheat varieties with high Zn efficiency for severely Zn-deficient conditions.
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    Dynamics of nutrient remobilisation from seed of wheat genotypes during imbibition, germination and early seedling growth (1997)
    Springer
    Plant and soil 197 (1997), S. 271-280 
    Details
    ISSN: 1573-5036
    Keywords: genotype ; remobilisation ; seed nutrient content ; seedling ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The changes in nutrient content of grain tissues and seedling parts of two wheat genotypes (Triticum aestivum L., Excalibur and Janz) with low or high seed Zn content were followed from imbibition to early seedling development (12 days). The grains were separated into seed coat, endosperm and embryo, while the seedlings were separated into roots and shoots. The dry weight of the seed coat did not change throughout the experimental period, whereas the endosperm weight declined rapidly from day 4 onward. The weight of embryo did not show any difference between and within cultivars. About a half of seed Zn was remobilised into shoot and roots during 12 days of growth, regardless of the initial seed Zn content in both genotypes. The seed coat contained 55–77% of the total seed nutrients in the two wheat genotypes, except in the case of S (around 40%). Manganese, Fe, Ca, K, and P were remobilised effectively from the seed coat as well as from the endosperm, while remobilisation of Zn and Cu was relatively less from the seed coat than from the endosperm. After 10 days of growth, all nutrients monitored were completely remobilised from the endosperm. Remobilised K was directed primarily into shoots; an increase in K content in shoots was relatively higher than the accumulation of dry matter, with a consequent increase in K concentration in shoot tissue. The remobilisation of some nutrients (eg. Fe, Ca and Zn) from various grain tissues during inbibition, germination and early growth is different from the remobilisation in more mature plants.
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    URL: http://dx.doi.org/10.1023/A:1004227400287
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    Interactions of rhizobia with rice and wheat (1997)
    Springer
    Plant and soil 194 (1997), S. 115-122 
    Details
    ISSN: 1573-5036
    Keywords: Azorhizobium caulinodans ; crack entry ; intercellular colonization ; naringenin ; rice ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Recently, evidence has been obtained that naturally occurring rhizobia, isolated from the nodules of non-legume Parasponia species and from some tropical legumes, are able to enter the roots of rice, wheat and maize at emerging lateral roots by crack entry. We have now investigated whether Azorhizobium caulinodans strain ORS571, which induces root and stem nodules on the tropical legume Sesbania rostrata as a result of crack entry invasion of emerging lateral roots, might also enter rice and wheat by a similar route. Following inoculation with ORS571 carrying a lacZ reporter gene, azorhizobia were observed microscopically within the cracks associated with emerging lateral roots of rice and wheat. A high proportion of inoculated rice and wheat plants had colonized lateral root cracks. The flavanone naringenin at 10 and 10 M stimulated significantly the colonization of lateral root cracks and also intercellular colonization of wheat roots. Naringenin does not appear to be acting as a carbon source and may act as a signal molecule for intercellular colonization of rice and wheat by ORS571 by a mechanism which is nod gene-independent, unlike nodule formation in Sesbania rostrata. The opportunity now arises to compare and to contrast the ability of Azorhizobium caulinodans with that of other rhizobia, such as Parasponia rhizobia, to intercellularly colonize the roots of non-legume crops.
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    URL: http://dx.doi.org/10.1023/A:1004283819084
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    Biological nitrogen fixation in non-leguminous field crops: Facilitating the evolution of an effective association between Azospirillum and wheat (1997)
    Springer
    Plant and soil 194 (1997), S. 65-79 
    Details
    ISSN: 1573-5036
    Keywords: Azospirillum brasilense ; evolution ; nifA-lacZ ; nifH-lacZ ; nitrogen fixation ; para-nodules ; symbiosis ; wheat ; 2 ; 4-D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Recent advances towards achieving significant nitrogen fixation by diazotrophs in symbioses with cereals are reviewed, referring to the literature on the evolution of effective symbioses involving rhizobia and Frankia as microsymbionts. Data indicating that strains of Acetobacter and Herbaspirillum colonizing specific cultivars of sugarcane as endophytes make a significant contribution to the nitrogen economy of this crop improves the prospects that similar associative systems may be developed for other gramineous species such as rice and wheat. By contrast, the transfer of nodulation genes similar to those in legumes or Parasponia to achieve nodulation in crops like rice and wheat is considered to be a more ambitious and distant goal. Progress in developing an effective associative system for cereals has been materially assisted by the development of genetic tools based on the application of lacZ and gusA fusions with the promoters of genes associated with nitrogen fixation. These reporter genes have provided clear evidence that ‘crack-entry’ at the points of emergence of lateral roots or of 2,4-D induced para-nodules is the most significant route of endophytic colonization. Furthermore, using the laboratory model of para-nodulated wheat, there is now evidence that the ability of azospirilla and other nitrogen fixing bacteria to colonize extensively as endophytes can be genetically controlled. The most successful strain of Azospirillum brasilense (Sp7-S) for endophytic colonization and nitrogen fixation in wheat seedlings is a mutant with reduced exopolysaccharide production. Most other strains of azospirilla do not colonize as endophytes and it is concluded that though these are poorly adapted to providing nitrogen for the host plant, they are well adapted for survival and persistence in soil. A research program combining the study of endophytic colonization by azospirilla with an examination of the factors controlling the effectiveness of association (oxygen tolerance and nitrogen transfer) is now being pursued. It is proposed that a process of facilitated evolution of para-nodulated wheat involving the stepwise genetic improvement of both the prospective microsymbionts and the cereal host will eventually lead to effective nitrogen-fixing associations. In the attempt to achieve this goal, continued study of the endophytes occurring naturally in sugar cane and other grasses (e.g. Azoarcus sp.) should be of assistance.
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    URL: http://dx.doi.org/10.1023/A:1004260222528
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    Introduction to bread wheat (Triticum aestivum L.) and assessment for bread-making quality of alleles from T. boeoticum Boiss. ssp. thaoudar at Glu-A1 encoding two high-molecular-weight subunits of glutenin (1997)
    Springer
    Euphytica 93 (1997), S. 19-29 
    Details
    ISSN: 1573-5060
    Keywords: dough quality ; electrophoresis ; endosperm storage protein ; genetics ; gluten strength ; near isogenic line ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two alleles, Glu-A1r encoding high-molecular-weight (HMW) glutenin subunits 39+40 and Glu-A1s encoding HMW glutenin subunits 41+42, were introgressed to bread wheat (Triticum aestivum L.) cv. Sicco from two accessions of T. boeoticum Boiss. ssp. thaoudar (A genome species, 2n=2x=14). Alleles at Glu-A1 in current commercial bread wheats encode zero or one subunit, and alleles at the homoeoloci Glu-B1 and Glu-D1 encode a maximum of two subunits; hence the maximum number of subunits found in commercial wheats is five, whereas the lines incorporating Glu-A1r and Glu-A1s carry six. Using near-isogenic lines, the current results demonstrated that the introduction of Glu-A1r resulted in diminished dough stickiness and improved stability during mixing compared with Glu-A1a encoding subunit 1, and a small improvement in gluten strength as shown by the SDS- sedimentation test. The introduction of Glu-A1a also resulted in a small improvement in gluten strength predicted by the SDS-sedimentation test. Thus the alleles are of potential value in breeding programmes designed to improve bread-making quality.
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    URL: http://dx.doi.org/10.1023/A:1002991206350
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    An assessment of somaclonal variation as a breeding tool for generating herbicide tolerant genotypes in wheat (Triticum aestivum L.) (1997)
    Springer
    Euphytica 94 (1997), S. 335-340 
    Details
    ISSN: 1573-5060
    Keywords: somaclonal variation ; in vitro selection ; herbicides ; wheat ; triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The present work was initiated to assess tissue culture techniques as a means of generating and selecting herbicide tolerant genotypes of wheat through exploiting somaclonal variation. Callus was initiated from immature embryos of genetically defined varieties and subcultured onto selective media containing 5 μm, 10 μm and 50 μm concentrations of difenzoquat and atrazine. Plants were regenerated from all the selective media except that media containing the highest herbicide concentration. The progenies of the regenerated plants were tested as whole plants for their response to spray application of the herbicides. For difenzoquat, variation in response from extreme susceptibility to tolerance was observed. However, genetic characterization by progeny testing tolerant lines revealed that the induced variation was not heritable. No plants tolerant to atrazine were obtained. Overall, no clear evidence of heritable mutations was obtained. Alternative strategies to obtain herbicide tolerant genotypes using cell culture techniques are discussed.
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    URL: http://dx.doi.org/10.1023/A:1002966309224
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    Can photosynthesis respond to short-term fluctuations in atmospheric carbon dioxide? (1997)
    Springer
    Photosynthesis research 51 (1997), S. 179-184 
    Details
    ISSN: 1573-5079
    Keywords: chlorophyll fluorescence ; FACE ; global change ; photosynthesis ; Photosystem II ; quantum yield ; quenching analysis ; rising CO2 concentration ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rapid and irregular variations of atmospheric CO2 concentrations (ca) occur in nature but are often very much more pronounced and frequent when artificially enriching CO2 concentrations in simulating the future atmosphere. Therefore, there is the danger that plant responses at elevated CO2 in fumigation experiments might reflect the increased frequency and amplitude of fluctuation in concentration as well as the increase in average concentration. Tests were conducted to determine whether the photosynthetic process could sense such fluctuations in ca. Instantaneous chlorophyll fluorescence (Ft) was monitored for wheat leaves (Triticum aestivum cv. Hereward) exposed to ca oscillating symmetrically by 225 μmol mol-1 about a ca set point concentration of 575 or 650 μmol mol-1. No Ft response was detected to half-cycle step changes in ca lasting less than two seconds, but at half-cycles of two seconds or longer, the response of Ft was pronounced. In order to determine the in vivo linear electron transport rate (J) the O2 concentration was maintained at 21 mmol mol-1 to eliminate photorespiration. J which is directly proportional to the rate of CO2 uptake under these conditions, was not significantly changed at half-cycles of 30 s or less but was decreased by half-cycles of 60 s or longer. It was inferred that if duration of an oscillation is less than 1 minute and is symmetrical with respect to mean CO2 concentration, then there is no effect on current carbon uptake, but oscillations of 1 minute or more decrease photosynthetic CO2 uptake in wheat.
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    Coactivators and TAFs of transcription activation in wheat (1997)
    Springer
    Plant molecular biology 35 (1997), S. 1037-1043 
    Details
    ISSN: 1573-5028
    Keywords: coactivators ; gene regulation ; plants ; TAFs ; transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcription regulation often activates quiescent genes in a tissue-specific or developmental manner. Activator proteins bind to a DNA sequence upstream of the promoter, interact with the general transcription proteins via bridging proteins, and elevate transcription levels. One group of bridging proteins, the coactivators, have been characterized in animals as polypeptides tightly associated with the general transcription factor TATA-binding protein (TBP). They are referred to as TAFs (TBP-associated factors), and together with TBP comprise general transcription factor IID. We provide biochemical evidence that wheat IID contains coactivators. An activator protein with an acidic activation domain facilitates the binding of IID to the template, and potentiates activated in vitro transcription with wheat IID, but not with wheat TBP. Using antibodies to wheat TBP, we demonstrate that wheat IID also contains TAFs. This is the first demonstration that a plant contains coactivators and TAFs.
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    METAL EXTRACTABILITY AND AVAILABILITY IN A SOIL AFTER HEAVY APPLICATION OF EITHER NICKEL OR LEAD IN DIFFERENT FORMS (1997)
    Springer
    Water, air & soil pollution 94 (1997), S. 33-44 
    Details
    ISSN: 1573-2932
    Keywords: nickel ; lead ; other metals ; salt ; sewage sludge ; acidifying agent (wastewater from olive oil processing) ; soil ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A greenhouse experiment using soil was conducted to investigate the effects of the addition of different forms of either nickel or lead, together with an acidifying agent, on the distribution of Ni, Pb, Zn, Cu and Mn in wheat plants, and on the post-harvest extractability of these elements in the soil. Two treatments consisting of soil alone or soil mixed with sewage sludge at a rate of 200 Mg ha−1 were used as controls. Nickel (400 mg kg−1) or lead (1600 mg kg−1) was added to the soil as an inorganic salt or mixed previously with sewage sludge. Six further treatments including an acidifying agent (wastewater from olive oil processing: alpechin) were also prepared. Wheat (Triticum aestivum L. var. Mesa) plants were harvested 75 d after germination. Dry matter yield of wheat was increased by the addition of sewage sludge. No reductions in yield were observed after the addition of nickel or lead. Nickel concentration and uptake by wheat, and extractability from soil, were higher when the sewage sludge enriched in nickel was added to soil. This effect was enhanced when the acidifying agent was also added. In contrast, lead availability was higher after the addition of inorganic Pb to soil. The addition of both forms of Ni enhanced Zn, Cu and Mn uptake by the plant, whereas the addition of lead increased Zn and Cu. After harvesting, increases in extractable Zn and Cu in the soil were observed only in treatments with sewage sludge, and not after the addition of Ni or Pb, or after the addition of the acidifying agent. Decreasing the pH of the soil with the acidifying agent tended to increase Mn uptake by wheat, and Mn extractability from the soil after harvesting.
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    Metal extractability and availability in a soil after heavy application of either nickel or lead in different forms (1997)
    Springer
    Water, air & soil pollution 94 (1997), S. 33-44 
    Details
    ISSN: 1573-2932
    Keywords: nickel ; lead ; other metals ; salt ; sewage sludge ; acidifying agent (wastewater from olive oil processing) ; soil ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A greenhouse experiment using soil was conducted to investigate the effects of the addition of different forms of either nickel or lead, together with an acidifying agent, on the distribution of Ni, Pb, Zn, Cu and Mn in wheat plants, and on the post-harvest extractability of these elements in the soil. Two treatments consisting of soil alone or soil mixed with sewage sludge at a rate of 200 Mg ha−1 were used as controls. Nickel (400 mg kg−1) or lead (1600 mg kg−1) was added to the soil as an inorganic salt or mixed previously with sewage sludge. Six further treatments including an acidifying agent (wastewater from olive oil processing: alpechin) were also prepared. Wheat (Triticum aestivum L. var. Mesa) plants were harvested 75 d after germination. Dry matter yield of wheat was increased by the addition of sewage sludge. No reductions in yield were observed after the addition of nickel or lead. Nickel concentration and uptake by wheat, and extractability from soil, were higher when the sewage sludge enriched in nickel was added to soil. This effect was enhanced when the acidifying agent was also added. In contrast, lead availability was higher after the addition of inorganic Pb to soil. The addition of both forms of Ni enhanced Zn, Cu and Mn uptake by the plant, whereas the addition of lead increased Zn and Cu. After harvesting, increases in extractable Zn and Cu in the soil were observed only in treatments with sewage sludge, and not after the addition of Ni or Pb, or after the addition of the acidifying agent. Decreasing the pH of the soil with the acidifying agent tended to increase Mn uptake by wheat, and Mn extractability from the soil after harvesting.
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    URL: http://dx.doi.org/10.1007/BF02407091
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    Mixotrophic productivity of the marine diatom Phaeodactylum tricornutum cultured with soluble fractions of rye, wheat and potato (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 349-351 
    Details
    ISSN: 1573-0972
    Keywords: Flour ; mixotrophic productivity ; Phaeodactylum tricornutum ; potato ; rye ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The marine microalga Phaeodactylum tricornutum was cultured semi-continuously with the soluble fractions of wheat, rye and boiled potato flours. Fifteen percent of the culture volume was renewed every 3 d. The cell productivities were 0.9×109 cells/l/d, 1.1×109 cells/l/d and 2.6×109 cells/l/d for wheat, rye and potato respectively. The productivity of the autotrophic control was 1.0×109 cell/l/d. When a soluble fraction of raw potato was added, the productivity was enhanced to 4.1×109 cells/l/d, 2.4 times higher than the autotrophic culture. The high productivity of P. tricornutum with the soluble fractions of Solanum tuberosum suggests its usefulness as a source of nutrients for the production of microalgal biomass.
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    Comparison of visual head blight ratings, seed infection levels, and deoxynivalenol production for assessment of resistance in cereals inoculated with Fusarium culmorum (1997)
    Springer
    European journal of plant pathology 103 (1997), S. 589-595 
    Details
    ISSN: 1573-8469
    Keywords: barley ; freezing blotter test ; Fusarium head blight ; mycotoxin ; oats ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seven spring wheat, 13 barley, 14 oats and 20 winter wheat genotypes were inoculated at flowering in 1993 and 1994 with mixed conidial suspensions of 8 isolates of Fusarium culmorum. Four weeks after inoculation, head blight was recorded in the field. After harvest, seed infection was assessed by a Freezing Blotter Test in the laboratory. Seed samples were also analyzed for deoxynivalenol (DON) content. Differences were found in head blight rating, the levels of infected seeds and the DON content between wheat, barley, and oats and between cultivars. Highly significant correlations were found between the percentage of heavily infected seed and the DON content. The weighted mean value of infected seeds and DON content were also significantly correlated. No significant correlation was found between head blight rating in the field and DON content. The level of infected seeds, as determined by the Freezing Blotter Test, was a better indication of the DON content in the seeds than the head blight rating. This visual assessment of levels of infected seeds gives a reliable estimate of resistance to Fusarium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008693213656
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    First survey on the natural occurrence of Fusarium mycotoxins in Bulgarian wheat (1996)
    Springer
    Mycopathologia 136 (1996), S. 47-52 
    Details
    ISSN: 1573-0832
    Keywords: Fusarium ; mycotoxins ; occurrence ; trichothecenes ; wheat ; zearalenone ; deoxynivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Wheat for human consumption (140 samples) was collected after harvest from all regions of Bulgaria. The 1995 crop year was characterized by heavy rainfall in the spring and summer months. The internal mycoflora of wheat samples was dominated by Fusarium spp. and Alternaria spp., and storage fungi were rarely present. The samples were analysed for contamination with Fusarium mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), T-2 Toxin (T-2), diacetoxyscirpenol (DAS), and zearalenone (ZEA), using enzyme immunoassay methods. DON and ZEA were the predominant toxins, with a contamination frequency of 67% and 69%, respectively. The average levels of these toxins in positive samples were 180 μg/kg (DON) and 17 μg/kg (ZEA), maximum concentrations were 1800 μg kg−1 and 120 μg kg−1, respectively. Acetyl derivatives of DON, namely 3-AcDON and 15-AcDON, were found in 2.1 % and 0.7% of the samples, at at maximum level of about 100 μg kg−1. Only one sample was positive for T-2 (55 μg/kg), DAS was not detected. This is the first report about the natural occurrence of a range of Fusarium mycotoxins in wheat for human consumption in Bulgaria.
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    URL: http://dx.doi.org/10.1007/BF00436660
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    Sustainable yield trends of irrigated maize and wheat in a long-term experiment on a loamy sand in semi-arid India (1996)
    Springer
    Nutrient cycling in agroecosystems 46 (1996), S. 225-234 
    Details
    ISSN: 1573-0867
    Keywords: long-term experiment ; maize ; wheat ; fertilizers ; farm yard manure ; weedicide application ; yield sustainability ; zinc deficiency ; nutrient uptake ; cropping sequence ; organic carbon build-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Long-term field experiments play an important role in understanding the complex interactions of plants, soils, climate and management and their effects on sustainable crop production. A long-term fertilizer experiment with maize-wheat-cowpea (fodder) is in progress since 1971 at Punjab Agricultural University farm Ludhiana, India. The experimental result for the first 21 years showed that application of N alone or in combination with P did not produce as much maize and wheat grains as the application of N, P and K together. Eight years after the start of the experiment, the optimal levels of N, P and K application (100% NPK) were unable to sustain the similar (maize) yield level as before because of Zn deficiency. Whereas in FYM amended plots the Zn deficiency did not appear and the higher crop yields could be sustained. The chemical control of weeds could not sustain the maize productivity at the same level as the manual removal of weeds. It was concluded that the high level of crop production can be sustained with the application of N, P and K under intensive cropping system provided deficiency of any of the micronutrient does not crop up. The deficiency of Zn is most likely to occur in semi-arid light textured alluvial soils under intensive cropping without the addition of farm yard manure/organic manures. In maize based cropping systems, manual control of weeds may be preferred to the chemical one. Addition of FYM in conjunction with 100% NPK is most beneficial both from bio-physical and economic point of view.
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    URL: http://dx.doi.org/10.1007/BF00420557
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    cDNA encoding a wheat (Triticum aestivum cv. Chinese Spring) glycine-rich RNA-binding protein (1996)
    Springer
    Plant molecular biology 30 (1996), S. 1301-1306 
    Details
    ISSN: 1573-5028
    Keywords: abscisic acid ; glycine-rich ; ribonucleoprotein ; RNA-binding protein ; RNP motif ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings. whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
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    URL: http://dx.doi.org/10.1007/BF00019560
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    Differential display-mediated rapid identification of different members of a multigene family, HSP16.9 in wheat (1996)
    Springer
    Plant molecular biology 31 (1996), S. 575-584 
    Details
    ISSN: 1573-5028
    Keywords: differential display ; heat shock proteins ; non-coding regions ; PCR ; reverse transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific probes from 3′ non-coding regions of different members can assist in the fast retrieval and characterization of individual members of a multigene family. We used the recent technique of differential display for the same purpose. As an example of a multigene family in plants, we selected a heat shock protein gene family, HSP16.9 from wheat, with estimated 12 members. We modified the original differential display technique for selective amplification of the 3′ non-coding regions of different wheat HSP16.9 genes by replacing the random 10-mer in the original method with a conserved HSP16.9 gene family-specific primer. Sixteen cDNA fragments from these experiments were sequenced and they represent 8 different members of a 12 member gene family. Our succes can be attributed to shorter 3′ non-coding regions that are typical of higher-plant genes and use of highly conserved gene family-specific primer in these experiments. This modified differential display technique can be of general application to other plant systems where cloning of the different members of a gene family is desired.
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    URL: http://dx.doi.org/10.1007/BF00042230
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    A pollen allergen-encoding gene is expressed in wheat ovaries (1996)
    Springer
    Plant molecular biology 32 (1996), S. 435-445 
    Details
    ISSN: 1573-5028
    Keywords: male sterility ; ovary ; parthenogenetic lines ; pollen allergen ; subtractive hybridization ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To isolate genes specifically expressed at the initiation of plant embryo development we have applied a sensitive subtractive hybridization technique for three isogenic wheat lines of the so-called ‘Salmon system’ with either zygotic or autonomous embryo development. Here we present a gene sequence showing a high homology to grass pollen allergens of type II/III thought to be expressed in pollen tissue only. Surprisingly, the pollen allergen-like sequence, designated Tri a III, is also expressed in gynoecia of the sexual, male fertile wheat line ‘(aestivum)-Salmon’, whereas the two parthenogenetic and male sterile wheat lines ‘(caudata)-Salmon’ and ‘(kotschyi)-Salmon’ completely lack any Tri a III transcript. Our data suggest a positive correlation between the expression of this clone and the manifestation of male fertility. Northern and in situ hybridization analysis revealed that, in addition to its presence in pollen, Tri a III is expressed in the parenchymatous tissue of ‘(aestivum)-Salmon’ ovaries exclusively at the day of anthesis. This precise temporal and spatial expression pattern suggests a more general function of the pollen allergen-like sequence Tri a III not limited to the exhibition of allergens in pollen grains.
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    URL: http://dx.doi.org/10.1007/BF00019095
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    A novel plant peptidyl-prolyl-cis-trans-isomerase (PPIase): cDNA cloning, structural analysis, enzymatic activity and expression (1996)
    Springer
    Plant molecular biology 32 (1996), S. 493-504 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; expression ; FKBP ; PPIase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel cDNA encoding for a peptidyl-prolyl-cis-trans-isomerase (PPIase) belonging to the FK506-binding protein (FKBP) family was isolated from wheat. It contains an open reading frame of 559 amino acids and it represents the first plant FKBP-PPIase to be cloned. It possesses a unique sequence which is composed of three FKPB-like domains, in addition to a putative tetratricopeptide repeat (TPR) motif and a calmodulin-binding site. The recombinant FKBP-PPIase expressed in and purified from Escherichia coli exhibits PPIase activity that is efficiently inhibited by the immunosuppressive drugs FK506 and rapamycin. Northern blot analysis showed that wheat FKBP was found mainly in young tissues. Polyclonal antibodies revealed the presence of cross-reacting proteins in embryos, roots and shoots. The unique structural features, the enzymatic activity and the presence of putative isoforms in wheat tissues indicate the possibility of the involvement of wheat PPIase in essential biological functions, similar to other members of the FKBP gene family.
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    URL: http://dx.doi.org/10.1007/BF00019101
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    Accelerated production of transgenic wheat (Triticum aestivum L.) plants (1996)
    Springer
    Plant cell reports 16 (1996), S. 12-17 
    Details
    ISSN: 1432-203X
    Keywords: Triticum aestivum L. ; wheat ; transformation ; biolistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a method for the accelerated production of fertile transgenic wheat (Triticum aestivum L.) that yields rooted plants ready for transfer to soil in 8–9 weeks (56–66 days) after the initiation of cultures. This was made possible by improvements in the procedures used for culture, bombardment, and selection. Cultured immature embryos were given a 4–6 h pre-and 16 h post-bombardment osmotic treatment. The most consistent and satisfactory results were obtained with 30 μg of gold particles/bombardment. No clear correlation was found between the frequencies of transient expression and stable transformation. The highest rates of regeneration and transformation were obtained when callus formation after bombardment was limited to two weeks in the dark, with or without selection, followed by selection during regeneration under light. Selection with bialaphos, and not phosphinothricin, yielded more vigorously growing transformed plantlets. The elongation of dark green plantlets in the presence of 4–5 mg/l bialaphos was found to be reliable for identifying transformed plants. Eighty independent transgenic wheat lines were produced in this study. Under optimum conditions, 32 transformed wheat plants were obtained from 2100 immature embryos in 56–66 days, making it possible to obtain R3 homozygous plants in less than a year.
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    URL: http://dx.doi.org/10.1007/BF01275440
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    Growth promotion ability of zoysiagrass rhizosphere fungi in consecutive plantings of wheat and soybean (1996)
    Springer
    Mycoscience 37 (1996), S. 163-168 
    Details
    ISSN: 1618-2545
    Keywords: consecutive planting ; Phoma sp. ; plant growth-promotion ; potting medium ; soybean ; wheat ; zoysiagrass rhizosphere fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several isolates ofPhoma sp., certain nonsporulating fungi, as well asPenicillium andTrichoderma, all isolated from zoysiagrass rhizosphere, promoted growth of wheat and soybean under greenhouse conditions. However, the ability of these rhizosphere fungi to enhance plant growth varied with the crop tested. For example, most of the fungi effectively promoted the growth of wheat, whereas only a few fungi were effective on soybean. In consecutive plantings of wheat and soybean grown in soil previously infested with these zoysiagrass rhizosphere fungi, the growth promotion ability of the fungi was lowered. However, addition of fresh potting medium appeared to restore their growth-promotive effects. It appears that the activation of plant growth-promoting fungi in soil might depend on the availability of organic substrates to colonize, as evidenced by the promotion of plant growth.
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    URL: http://dx.doi.org/10.1007/BF02461341
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    Characterization of the translocated chromosome using fluorescencein situ hybridization and random amplified polymorphic DNA on twoTriticum aestivum—Thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus (1996)
    Springer
    Chromosome research 4 (1996), S. 583-587 
    Details
    ISSN: 1573-6849
    Keywords: in situ hybridization ; intermediate wheatgrass ; translocation ; virus resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescencein situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines withThinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of theTh. intermedium chromosomes carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using theph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES ofTh. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL fromTh. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosome recombination.
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    URL: http://dx.doi.org/10.1007/BF02261721
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    Nutrient inflow and root proliferation during the exploitation of a temporally and spatially discrete source of nitrogen in soil (1996)
    Springer
    Plant and soil 178 (1996), S. 185-192 
    Details
    ISSN: 1573-5036
    Keywords: ammonium ; heterogeneity ; inflow ; localised nutrient ; mineralisation ; 15N ; nitrate ; organic residue ; proliferation ; roots ; soil ; uptake ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To obtain nutrients mineralised from organic matter in the soil, plants have to respond to its heterogeneous distribution. We measured the timing of nitrogen uptake by wheat from a localised, 15N labelled organic residue in soil, as well as the timing of changes in root length density. We calculated the rates of N uptake per unit root length (inflows) for roots growing through the residue and for the whole root system. A stimulated local inflow appeared to be the main mechanism of exploitation of the residue N during the first five days of exploitation. 8% of the N that the plants would ultimately obtain from the residue was captured in this period. Roots then proliferated in the residue. This, together with a rapidly declining N inflow, contributed to the capture, over the next seven days, of 63% of the N that the plants derived from the residue. After that time, massive root proliferation occurred in the residue, but relatively little further N was captured.
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    URL: http://dx.doi.org/10.1007/BF00011582
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    Yield and water use of wheat (Triticum aestivum) in a Mediterranean environment: Cultivar differences and sowing density effects (1996)
    Springer
    Plant and soil 181 (1996), S. 251-262 
    Details
    ISSN: 1573-5036
    Keywords: evapotranspiration ; grain yield ; irrigation ; plant density ; water use efficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Yield of eight wheat cultivars was evaluated under rainfed and irrigated conditions in a Mediterranean environment. Variation in grain yield resulted from variation in both aboveground biomass production and in harvest index. Under rainfed compared to irrigated conditions, grain yield, biomass and days to heading were decreased, whereas harvest index was increased. Grain yield of the different cultivars under rainfed conditions correlated with that under irrigated conditions in one of the two years. Among cultivars, harvest index under rainfed and irrigated conditions were correlated in both years. Water was used more efficiently for biomass production, and equally efficiently for grain production, under irrigated compared to rainfed conditions. Under rainfed conditions, crop water use efficiency was higher for cultivars developed for rainfed environments than for those developed for high-rainfall or irrigated environments. Cultivars with low-rainfall target environments had the lowest evapotranspiration under rainfed conditions. Under rainfed conditions, differences between the cultivar groups in crop water use efficiency corresponded with trends in water use efficiency of individual plants and with the ratio of photosynthesis to transpiration, measured on plants grown in a growth room. Early in the season, water was used more efficiently for biomass production at high sowing densities than at low sowing densities. Through faster biomass production and ground cover a smaller proportion of the evapotranspired water was lost in soil evaporation and a larger proportion was transpired. However, the net effect was a greater water use in the early phases of growth and consequently a lower water availability later in the season, leading to similar yields regardless of sowing density.
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    Genotypic variation in zinc efficiency and resistance to crown rot disease (Fusarium graminearum Schw. Group 1) in wheat (1996)
    Springer
    Plant and soil 186 (1996), S. 219-226 
    Details
    ISSN: 1573-5036
    Keywords: crown rot ; Fusarium ; fungal disease ; genotypes ; wheat ; zinc ; zinc efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A crown rot disease in wheat caused by the fungusFusarium graminearum Schw. Group 1 is a widespread problem in chronically Zn-deficient Australian soils. A link between crown rot and Zn deficiency was established by Sparrow and Graham (1988). This paper reports a test of a further hypothesis, that wheat genotypes more efficient at extracting zinc from low-zinc soils are more resistant to infection by this pathogen. Three wheat cultivars (Excalibur, Songlen and Durati) of differential Zn efficiency were tested at three zinc levels (0.05, 0.5 and 2.0 mg Zn kg−1 of soil) and three levels ofF. graminearum S. Group 1 inoculum (0.1 g and 0.3 g kg−1 live chaff-inoculum and control having 0.1 g kg−1 dead chaff inoculum). Six weeks after sowing dry matter production of shoots and roots was decreased byFusarium inoculation at 0.05 mg and 0.5 mg kg−1 applied Zn.Fusarium inoculum at 0.1 g was as effective as 0.3 g kg−1 for infection and decreasing dry matter. The infection at the basal part of culm decreased significantly by increasing the rate of Zn application. Excalibur, a Zn-efficient cultivar (tolerant to Zn deficiency) produced significantly more shoot and root dry matter, and showed less disease infection compared with Zn-inefficient cultivars (Durati and Songlen) at low (0.05 mg Zn kg−1 soil) and medium (0.5 mg Zn kg−1 soil) Zn fertilization rates. Higher rate of Zn fertilization (2.0 mg Zn kg−1 soil) reduced the disease level in Durati to the level of Excalibur but the disease level of Songlen was still high, indicating its high Zn requirement and or sensitivity to crown rot. The data on Zn uptake show that Excalibur, being Zn-efficient, was able to scavenge enough Zn from Zn-deficient soil, we suggest that besides sustaining growth Excalibur was able to build and maintain resistance to the pathogen; inefficient cultivars needed extra Zn fertilization to achieve performance comparable to that of Excalibur. The present study indicates that growing Zn-efficient cultivars of wheat along with judicious use of Zn fertilizer in Zn-deficient areas where crown rot is a problem may sustain wheat production by reducing the severity of the disease as well as by increasing the plant vigour through improved Zn nutrition. ei]Section editor: R Rodriques-Kalana
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    Reaction of tritordeum to Fusarium culmorum and Septoria nodorum (1996)
    Springer
    Euphytica 88 (1996), S. 165-174 
    Details
    ISSN: 1573-5060
    Keywords: wheat ; tritordeum ; Hordeum chilense ; Fusarium culmorum ; Septoria nodorum ; scab ; deoxynivalenol ; ergosterol ; resistance ; Triticum spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.
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    URL: http://dx.doi.org/10.1007/BF00023887
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    Aneuploids as a key for new molecular cloning strategies: development of DNA markers by microdissection using Triticum aestivum-Aegilops markgrafii chromosome addition line B (1996)
    Springer
    Euphytica 89 (1996), S. 41-47 
    Details
    ISSN: 1573-5060
    Keywords: Aegilops markgrafii ; aneuploids ; chromosome specific library ; DOP-PCR ; microdissection ; molecular markers ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5α. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.
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    Inter-varietal chromosome substitution lines in wheat — revisited (1996)
    Springer
    Euphytica 89 (1996), S. 1-10 
    Details
    ISSN: 1573-5060
    Keywords: aneuploidy ; chromosome substitutions ; height ; wheat ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The difficulties in developing inter-varietal chromosome substitution lines in wheat are reviewed. The use of genetical, cytological and molecular markers is suggested as a way of overcoming them. These difficulties and the use of markers, as well as the need to develop duplicate lines to detect background variation, are described using the development and analysis of the Cappelle-Desprez (Bezostaya 1) chromosome substitution set as an example. The effects of substituting Bezostaya 1 chromosomes on final plant height and adult-plant resistance to yellow rust are reported. The large number of aneuploids and substitution lines available in wheat provides a tremendous, international resource, which should be exploited in the future.
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    URL: http://dx.doi.org/10.1007/BF00015712
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    Effects of homoeologous group 1 and 6 chromosomes of the Cappelle-Desprez (Bezostaya 1) substitution lines on aspects of bread-making quality of wheat (1996)
    Springer
    Euphytica 89 (1996), S. 17-25 
    Details
    ISSN: 1573-5060
    Keywords: Bread-making quality ; chromosome effects ; grain hardness ; SDS-sedimentation volume ; substitution lines ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The group 1 and 6 inter-varietal chromosome substitution lines of Cappelle-Desprez (Bezostaya 1) were intercrossed along with the donor and recipient varieties, Cappelle-Desprez and Bezostaya 1, to give 36 genetically different families. The analysis of the means of these families showed that variation in SDS-sedimentation volume fitted a predominantly additive model. There were no significant within or between chromosome interactions among the group 1 and 6 chromosomes. Nor was there any evidence for interactions between these chromosomes and those of the background. Significant dominance/within chromosome interactions amongst the background chromosomes were however detected. Some of the positive effects on SDS-sedimentation were associated with increased grain hardness. Chromosome effects on % grain protein were not correlated with SDS-sedimentation.
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    URL: http://dx.doi.org/10.1007/BF00015714
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    Comparative mapping and its use for the genetic analysis of agronomic characters in wheat (1996)
    Springer
    Euphytica 89 (1996), S. 27-31 
    Details
    ISSN: 1573-5060
    Keywords: agronomic characters ; QTL ; RFLP maps ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The advent of molecular marker systems has made it possible to develop comparative genetic maps of the genomes of related species in the Triticeae. These maps are being applied to locate and evaluate allelic and homoeoallelic variation for major genes and quantitative trait loci within wheat, and to establish the pleiotropic effects of genes. Additionally, the known locations of genes in related species can direct searches for homoeologous variation in wheat and thus facilitate the identification of new genes. Examples of such analyses include the validation of the effects of Vrn1 on chromosome 5A on flowering time in different crosses within wheat; the indication of pleiotropic effects for stress responses by the Fr1 locus on chromosome 5A; the detection of homoeologous variation for protein content on the homoeologous Group 5 chromosomes; and the detection of a new photoperiod response gene Ppd-H1 in barley from homoeology with Ppd2 of wheat.
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    URL: http://dx.doi.org/10.1007/BF00015715
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    The use of wheat aneuploids for the chromosomal assignment of microsatellite loci (1996)
    Springer
    Euphytica 89 (1996), S. 33-40 
    Details
    ISSN: 1573-5060
    Keywords: aneuploids ; automated DNA sequencer ; chromosomal location ; microsatellite marker ; PCR ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chromosomal assignment of 64 PCR-amplified microsatellite loci and 29 additional fragments amplified by the same primer pairs is described for bread wheat (Triticum aestivum). The distribution over the different chromosomes and chromosome arms appears to be random. The highest proportion of microsatellite loci is found on the B genome, followed by the A and D genome. About half of the primer pairs amplified unique fragments, while the other half amplified additional fragments. 25% of the primer pairs, mostly designed to clones of a PstI-library, amplify fragments on homoeologous chromosomes. In some cases, more than one fragment on a single chromosome or fragments on non-homoeologous chromosomes occurred. The use of an automated DNA sequencer accounts for the accurate resolution of multiple fragments and enables to differentiate between fragments, amplified by a single primer pair, with size differences as small as two base pairs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015716
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  • 199
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    The influence of flowering time genes on environmental adaptability in European wheats (1996)
    Springer
    Euphytica 89 (1996), S. 49-57 
    Details
    ISSN: 1573-5060
    Keywords: adaptability ; earliness per se ; photoperiod sensitivity ; vernalization sensitivity ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In order to obtain high levels of environmental adaptability in wheat varieties it is essential they flower at times appropriate to particular environmental conditions. The influence of three distinct genetic systems that together determine time of flowering is reviewed here. Vernalization genes are seen to be particularly important to winter wheats for their direct or indirect effects on winter hardiness. Vernalization genes play a minor role in determining flowering time in autumn sown winter wheats but insensitivity is essential if spring sown wheats are to flower. Day length sensitive photoperiod genes play a major role in determining flowering time and adaptability of autumn sown wheats. Insensitivity can promote yield advantages of over 35% in Southern European environments. 15% in Central Europe and offers benefits even in the UK. At present only a single allele of Ppd1 appears to have been introduced into commercial European wheat varieties. The merits of alternative Ppd1 alleles or different loci are discussed. The influence of earliness per se genes that determine flowering time independently of environmental stimuli is less well documented than the effect of photoperiod and vernalization genes. It is likely that genes on chromosomes belonging to groups 2, 3, 4, 6 and 7 may act to modify flowering time independently of environmental stimuli probably by determining numbers of vegetative and floral primordia being initiated or the rate of initiation of the primordia. Earliness per se genes appear to be widespread in European wheats and play a significant role in determining the exact time plants flower.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015718
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  • 200
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    The relationships between the dwarfing genes of wheat and rye (1996)
    Springer
    Euphytica 89 (1996), S. 69-75 
    Details
    ISSN: 1573-5060
    Keywords: dwarfing genes ; gene mapping ; GA insensitivity ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The improvement of lodging resistance by introducing major dwarfing genes, classified either as GA insensitive or GA sensitive, is one of the main strategies chosen by cereal breeders. In the present paper the current knowledge about the genetics, chromosomal localisation and the homoeoallelic relationships of the dwarfing genes in wheat and rye is reviewed. The confusing system of the symbolisation of the GA insensitive dwarfing genes/alleles in wheat is discussed and a nomenclature based on rules for gene symbolisation in wheat is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015721
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