ALBERT

Notes: Data are presented for the fourth (1979) and fifth (1980) harvest years of a trial in which four levels of N fertilizer (0, 30, 60 and 90 kg N ha−1) were applied each spring to swards with grass (perennial ryegrass cv. Barlenna) and each one of four cultivars of while clover (Blanca, Sabeda, Olwen and S100) or grass alone. Results from the first three years have been published.Dry matter (DM) harvested dropped from the fourth to fifth years over all swards by 1 −5 to 2 0 t ha−1 but response to N was maintained (17.4 and 24.4 kg DM (kgN)−l in response to 90 kg N ha−1 in 1979 and 1980 respectively) despite reductions in summer yields relative to unfertilized swards. Blanca swards produced significantly less DM harvested than all other cultivars in 1979 and all cultivars in 1980 except for Olwen. Clover DM harvested continued to fall from 1977, the mean for the unfertilized treatment in 1980 being 55% of that in 1979. Up to 1980 Sabeda swards produced more clover DM harvested than Blanca swards. Linear regressions between annual clover content at zero N and at each spring N level for each sward type over 5 harvest years were very highly significant. It is concluded that all cultivars used responded similarly to spring N. Further work to develop a method for predicting the effect of spring N on clover content of given swards is required.

Notes: Preliminary experiments were conducted to assess the winter hardiness of representative cultivars or populations of Festuca rubra, Holcus lanatus and Agrostis spp. in comparison with a hardy (Premo) and a susceptible (Grasslands Ruanui) cultivar of Lolium perenne. Two controlled-environment tests and a field experiment including two cutting and two fertilizer managements are reported.Grasslands Ruanui andH lanatus suffered most damage in all tests. In the controlled-environment experiments, Premo was at least as hardy as the F. rubra and Agrostis cultivars but in the field Premo was more susceptible. Spring growth of Premo was affected more by the cutting and fertilizer managements applied the previous summer and autumn than was spring growth of F. rubra or Agrostis spp.It was concluded that, whereas use off. F. rubra or Agrostis spp. should not be limited by fears of winter damage, poor winter hardiness may limit the value of H. lanatus for hill and upland reseeding.

Notes: Patterns of defoliation in perennial ryegrass-dominated swards were examined in two experiments. In Experiment I measurements were made on four swards after a single overnight grazing. In Experiment 2 observations were made repeatedly on marked tillers in two continuously stocked swards which were grazed down over a 24-d period. In both experiments the chance of defoliation was related to lamina height both within and between age classes. Lamina angle was measured in Experiment 2 and within age classes the chance of defoliation was also independent of lamina angle. In Experiment 1 tillers most frequently had both of the two youngest laminae grazed; in Experiment 2 tillers with only lamina 1 grazed occurred most frequently, but defoliation of the two youngest laminae was again common.The different frequencies of grazing of the different classes of laminae is interpreted as being a function of their frequency of occurrence within the grazed layer at the top of the sward. The pseudostem apparently restricted the depth of the grazed layer in the very short swards of Experiment 2.Grazing behaviour in relation to sward structure and the patterns of defoliation and their implications for herbage production are discussed.

Notes: Perennial ryegrass and white clover were grown in boxes with either no competition, root competition only, shoot competition only, or both root and shoot competition between them, Boxes received either no nitrogen or 200 kg N ha−1 as a split application. The experiment was harvested at 8, 12, 16 and 20 weeks after sowing. Perennial ryegrass was more competitive than white clover throughout the experiment and its overall competitive ability increased with Lime. The effects of root competition were greater than those of shoot competition at the first harvest, with both forms of competition having similar affects at the second harvest, while at the third and final harvests shoot competition had greater effects than root competition. At the later harvests N application increased the overall competitive ability of perennial ryegrass, relative to white clover, mainly owing to the increase in its shoot competitive ability.

Notes: Inflorescence-bearing tillers of Italian ryegrass (Lolium multiflorum cv. RvP) were dried in controlled temperature, relative humidity and air speed conditions chosen to be within the range likely to occur in a hay swath drying in the field. After drying for increasing intervals the tillers were separated into inflorescence, exposed flowering stem, leaf-laminae, leaf-sheath and flowering stem enclosed by leaf-sheath and the drying rates of these components in addition to that of whole tillers were measured. There were large differences in drying rate between components, but the differences were similar over the range of drying conditions employed. The drying rate of the inflorescence as well as that of stem enclosed by leaf-sheath was found to be very low and that of leaf-laminae and leaf-sheath very high.

Notes: Book reviews in this article: Genetic Resources of Forage Plants Edited by J. G. McIvor and R. A. Bray Nutritional Limits to Animal Production from Pastures Edited by J. B. Hacker Herbivory. The Dynamics of Animal-Plant Interactions By Michael J. Crawley

Notes: Forty hill farms in Northern Ireland were surveyed to obtain information on stocking rates and output and to identify factors affecting output. The mean farm area of 194 ha was composed of 155 ha hill land and 39 ha inbye land with a mean stocking rate of 0–7 cow-equivalents ha−1.On the average farm most of the grassland (88%) was over 20 years old. The preferred species content of the swards averaged 22% over the whole farm and 42% in cut swards. This latter result may be attributed to the relatively high level of fertilizer N applied to these swards (131 kg ha−1) compared to the mean application rate over the whole farm of only 29 kg N ha−1 and 93 kg ha−1 applied to the inbye land.Output data calculated as utilized metabolizable energy (UME) per unit area for the whole farm and separately for hill and inbye components showed that although only 21% of the farm area was inbye land almost half the annual total metabolizable energy requirements of stock had to be met by grazing inbye. The capacity of herbage to meet stock energy demands was much lower on the hill with supplementary feed having to be provided over the winter.Calculated output from inbye land was 39 GJ ha−1 compared to 7 GJ ha−1 from the hill and 15 GJ ha−1 from the whole farm. UME output of the whole farm was negatively correlated with farm size (r=−0 55) and positively correlated with stocking rate (r = 0 89), applied N level (r = 0 63)

Notes: Forage-harvested crops of perennial ryegrass (200 g DM kg−1) and red clover (163 g DM kg−1) were inoculated with cultures of Streptococcus durans str. 1024, Lactobacillus acidophilus sir. 2356 and Lactobacillus plantarum str. 6, and 1:1 mixtures of the Streptococcus and each of the lactobacilli, at rates of 106 and 106 (g fresh weight)−1 and ensiled in laboratory silos for up to 128 d. The possible influence of these bacteria on fermentation was examined in relation to formic acid at rates of application of 0 and 2.3 g(kg fresh weight)−1, None of the cultures or culture mixtures, either at the low or high rate of application, had any notable influence on microbial development, the rate of acidification or promotion of the homolactic fermentation; they tended to exacerbate loss of insoluble nitrogen and deamination. All silages were well-preserved with no butyric acid being detected, in spite of pH increase during storage. Only formic acid had any consistent and desirable effect on preservation.

Notes: Book reviews in this article: Grass: its Production and Utilization Edited by W. Holmes Fream's Agriculture Edited by C. R. W. Spedding

Notes: The results or estimations of in vitro digestibility of three standard herbage samples, analysed weekly over about 48 weeks per year, have been examined using time-series methods of Box and Jenkins to separate seasonal and non-seasonal trends. The study includes data from March 1971 to February 1981. The dominant feature of variability is the seasonal component with a cycle of 52 weeks; no other significant cycles of longer periods were detected. The model of Box and Jenkins (1976) was found to be the most appropriate in describing the three series. A decrease in the in vitro digestibility of the low standard, which is 10 years old, occurred over the last year or so, although the reasons for this apparent storage effect are unknown.

Notes: Salts of formic, acetic and propionic acids and glutaraldehyde were subjected to an assay with representatives of the major groups of silage micro-organisms at pH 4·0, 4·5, 5·0 and 6·0. The antimicrobial spectra of the salts suggested that they are most effective against the least desirable components of the bacterial flora of silage, coliforms, Clostridia and bacilli, and would create conditions for a desirable fermentation in practice. In many instances the activity of the salts was better than the corresponding free acid and it was postulated that, if supplemented with acid, aerobic stability of silage might also be improved. Glutaraldehyde, whilst being less selective as an antimicrobial agent, possesses properties which would encourage a lactic-acid fermentation in silage.

Notes: Previous results with red clover (Trifolium pratense) leaflets, leaf petioles and stems indicated that the epidermis continued to impose a barrier to water loss at low water contents. Further experiments with red clover leaf petioles and stems suggest that cuticular wax is the epidermal component concerned and that treatments which remove or merely modify this will also increase drying rate. However, when treatments to reduce epidermal resistance were applied to pseudostems and flowering stems of Italian ryegrass (Lolium multiflorum) the initial increase in drying rate produced was not sustained at low water contents. The pseudostems consisted of three layers of leaf material rolled one within the other and the true flowering stems were surrounded by one leaf sheath. Their pattern of response to treatments suggested that these affected only the outer sheath, which produced an initial acceleration in drying rate, but that this was not sustained after the outer sheath was dry because the lower untreated layers dried in a similar way to untreated controls.The results suggest the possibility of devising practical treatments to increase drying rate at low water contents with crops containing a high proportion of leaf or stem not surrounded by leaf sheath. Devising such treatments for crops with a high proportion of grass pseudostem or flowering stem surrounded by leaf sheath will be considerably more difficult.

Notes: In this paper we develop a recursive algorithm to obtain the layer parameters of an elastic medium (density, P-wave velocity, S-wave velocity) from reflection coefficient matrices in terms of energy flux ratios for a non-normal incidence case. We define a layer impedance matrix, analogous to the impedance of an acoustic medium. Next we derive a matrix relationship between the layer impedance matrix of the n+ 1st layer and the reflection coefficient and parameter matrices of the nth layer. This relationship leads to recursively computing the parameters of the subsurface. We show that the elastic case—unlike the acoustic case—allows one to recover the layer parameters from the impedance matrix for non-normal incidence. The results of this work play a key role in the solution of the inverse problem with non-normal-incidence plane-wave seismic data when using a downward continuation technique.

Notes: Frequency-dependent attenuation of compressional waves within the earth has been estimated in the vicinity of wells from〈list xml:id="l1" style="custom"〉1spectral power ratios of the coherent events in separate time gates on the seismic section2matching a broadband synthetic trace with seismic data at the well, and3determining the operator that transforms one down(up) going pulse recorded in the well into another recorded at a deeper (shallower) level.The accuracy of estimation of all three methods was insufficient to estimate attenuation over small depth intervals, and it was not possible to distinguish between the contribution due to internal multiples and that of genuine absorption with much confidence. Spectral ratios from (1) showed a smoother variation with frequency—and one more consistent with other estimates—when they were compensated for the spectra of the reflectivities over the time gates employed, but they did not provide more than a broad indication of attenuation over a substantial depth interval. Approach (2) was hampered by the restricted durations over which synthetic trace and seismic data can be reliably matched; approach (3) gave the best results. Here matching is a much more powerful tool than the spectral-ratio techniques that are commonly applied since it can yield the form of the attenuation operator, i.e., both its amplitude and phase response, together with properly defined measures of its accuracy, while at the same time it minimizes the influence of noise and local interference effects at each recording level.For seismic target depths where internal multiple activity was low the logarithms of the amplitude responses of the estimated attenuation operators decreased approximately linearly with frequency and the phase responses showed no significant dispersion. Application of approach (3) to downgoing and upgoing waves estimated from a vertical seismic profile revealed the importance of changes in frequency-dependent geophone coupling and their effect on values of Q determined from downgoing pulses only.

Notes: From the wealth of information which can be deduced from VSP, the information most directly comparable to well logs is considered: P-wave and S-wave interval velocity, acoustic impedance, and the velocity ratio γ=Vs/Vp. This information not only allows better interpretation of surface seismic sections but also improves processing.For these results to be usable a number of precautions must be taken during acquisition and processing; the sampling in depth should be chosen in such a way that aliasing phenomena do not unnecessarily limit the spectra during the separation of upwards and downwards travelling waves. True amplitudes should be respected and checked by recording of signatures, and the interference of upwards and downwards travelling waves should be taken into account for the picking of first arrivals.The different steps in processing and the combination of results in the interpretation of surface seismic results are described with actual records.

Notes: The arrival-time curve of a reflection from a horizontal interface, beneath a homogeneous isotropic layer, is a hyperbola in the x - t-domain.If the subsurface is one-dimensionally inhomogeneous (horizontally layered), or if some or all of the layers are transversely isotropic with vertical axis of symmetry, the statement is no longer strictly true, though the arrival-time curves are still hyperbola-like. In the case of transverse isotropy, however, classical interpretation of these curves fails. Interval velocities calculated from t2 - x2-curves do not always approximate vertical velocities and therefore cannot be used to calculate depths of reflectors.To study the relationship between velocities calculated from t2 - x2-curves and the true velocities of a transversely isotropic layer, we approximate t2 - x2-curves over a vertically inhomogeneous transversely isotropic medium by a three-term Taylor series and calculate expressions for these terms as a function of the elastic parameters. It is shown that both inhomogeneity and transverse isotropy affect slope and curvature of t2 - x2-curves. For P-waves the effect of transverse isotropy is that the t2 - x2-curves are convex upwards; for SV-waves the curves are convex downwards. For SH-waves transverse isotropy has no effect on curvature.

Notes: A combination of ray theory and diffraction theory has been used to simulate reflections of seismic pulses from a full plane and reflections and diffractions from a half plane buried horizontally in earth models with linear increase of velocity with depth. Wave forms of signals reflected from a full plane in this case are modifications of the source pulse, even if the medium is assumed to be perfectly elastic. The extent of pulse modification increases with increase of the velocity gradient. The duration of the reflected pulse is always longer than that of the source pulse. The pulses diffracted from the edge of the half plane in such cases do not lie along hyperbolic curves in the time-offset plane. Diffracted signals can be observed only up to a limited distance from the edge of the half plane equalling half the horizontal range of the ray which is tangential to the half plane and has its end points at the level of the source and the receiver.

Notes: A new method with general applications for seismic data is presented for spectral extrapolation. The method gives a restored image that is optimum in the sense of minimum norm and can be adapted to incorporate any constraints on the reconstruction. As an illustration of the technique it is first applied to a synthesized (noise-free) image. Then it is shown that enhancement of a density log (run in a hole drilled entirely in Coal Measures rocks) is possible using constraints taken from the geologist's log. A synthetic trace with significantly improved event arrival times and general character is obtained.

Notes: The dynamic finite-element method allows frequency-dependent reflection and transmission coefficients to be computed for Love waves scattered by two-dimensional inhomogeneities in coal seams.Clean faults of zero hade angle show a throw-dependent cross-over frequency in reflection spectra, and throw-dependent conversion from fundamental to first higher mode energy in transmission spectra. Oblique faults show mode conversion in reflection spectra which is relatable to the fault hade angle by simple Huygens-theory models. Thin fracture zones or dykes normal to the seam show a reflection maximum when thickness of the zone is of order one quarter of the seam wave wavelength.Published field data from two known faults and a dyke are compared with the modeling results and support the belief that broad-band seam-wave data are capable of characterizing a seam discontinuity (throw, hade angle, dyke thickness) as well as locating it. Development of such procedures will require extensions to existing field practice and processing.This research was funded in part by the National Energy Research Development and Demonstration Council of Australia. The authors thank The Broken Hill Proprietary Company Limited for permission to quote from company case histories.

Notes: The response of a rectangular plate to the Crone PEM system has been investigated for different values of depth, dip and conductance parameters. The conductance aperture diagram is presented to estimate the conductance and depth to the top of a vertical sheet. Information regarding dip and depth may be obtained using the appropriate nomogram.

Notes: In areas where steep dips are encountered conventional practice in resistivity work has involved orienting arrays favourably in relation to the geological strike. In concealed conditions, however, the geological strike may not be known; moreover, strike may change with depth. Considerable advantage is to be gained, in such circumstances, by the use of crossed square arrays in that these yield orientationally insensitive resistivity measurements and also allow strike determinations and measurements of the effective vertical anisotropy.Two traverses of crossed square array observations are presented, together with one deeper sounding. The results show that, in favourable circumstances, reliable data on concealed strike directions can be obtained, and that the anisotropy findings greatly assist the subsequent interpretation. Model results pertinent to the field material are presented and discussed.

Notes: Absorption of seismic energy in the earth reduces amplitudes and changes phases of the propagating seismic waves. Amplitudes are usually recovered according to an estimated exponential decay curve, while phase distortions are generally disregarded. Therefore, accurate processing of seismic data requires a careful investigation of the relationship between absorption and phases.In this paper a procedure is suggested to achieve this goal, and some related topics are worked out. A method is outlined for computing synthetic seismograms and vertical seismic profiles with phase distortion due to absorption.The algorithm works in the frequency domain, and it provides for absorption according to the usual model of exponential decay of amplitude with distance. The absorption coefficient is a linear function of frequency and is related to the quality factor Q of the rocks. Complex seismic velocities are introduced and minimum-phase delay due to absorption is assumed for all cases considered.Methods for estimating Q profiles from seismic well surveys and seismic data are described. Comparison between field and synthetic data shows the effectiveness and benefits of the procedure. Some applications of the method to phase distortion recovery and wavelet processing are presented.

Notes: For helicopter-borne electromagnetic systems, the distance between the transmitting and the receiving coils is small compared with the altitude above ground. For this case, a major simplification can be made for the calculation of model curves. Some two-layer curves for the interpretation of frequency measurements are presented.A very simple procedure is demonstrated for the conversion of the relative secondary field into apparent resistivity and apparent distance for the mapping of airborne electromagnetic data. Furthermore, an approximation is described for the determination of the thickness and the resistivity of a layer lying on a perfectly conducting half-space.

Notes: A numerical method is given for calculating resistivity and induced polarization anomalies produced by a surface polarization model. Surface polarization is generated when a purely electronic conductor is located in an electrolyte environment. The system that develops on the boundary between the conductor and the electrolyte is described macroscopically by a net surface charge distribution and an electric double layer. An integral equation is derived for the potential by assuming that the electronic conductor forms an equipotential system and that the polarization impedance across the boundary is linear. The integral equation is solved by means of the method of subsections. As an application some numerical modeling results are presented. The surface impedance values used in calculations are based on laboratory measurements that are briefly described. Implications of the results for scale modeling are discussed.

Notes: White clover swards were grown in a freely ventilated glasshouse during August and September 1979. The uninterrupted growth of these swards, which were grown in Perlite with either nitrate or biologically fixed nitrogen, was studied from 5 weeks after sowing until peak dry weights of the live sward components occurred. The dry weight of leaf laminae, petioles, stolons, roots, nodules and dead material was determined weekly together with live leaf numbers and leaf lamina area. The pattern of dry matter accumulation of the yield components of the swards (live leaf laminae plus petioles) was characterized by an initial period of more or less exponential growth up to 6 weeks from sowing dominated by leaf lamina development. This was followed by a linear phase, when petiole weight increased rapidly and a final slowing down period terminated by peak yields of live DM 12 weeks after sowing.The weight of stolons, roots and nodules increased slowly during the period dominated by leaf lamina growth compared with the rapid increase thereafter, which resulted in a two- to three-fold increase in the weight of stolons, roots and nodules by the time the weights peaked. It is assumed that after the leaf area index of the sward had reached a plateau there was a limit to the assimilate supply to the sward components at a stage when they were all apparently competing for photosynthates. There was a progressive slowing down in the rate of dry matter accumu-

Notes: Grass silage made in late May from S24 perennial ryegrass was offered ad libitum to eight Ayshire cows in a 16-week feeding experiment. The silage had a DM concentration of 244 g kg−1, contained 163 g crude protein (kg DM)−1 with a ruminal degradability of 0.77 and had an in vitro DOMD concentration of 678 g kg−1. In addition, four concentrates each containing 167 g soya-bean meal kg−1 were consumed at a mean daily rate of 6.43 kg DM per cow. The soya-bean meal was either untreated, or ‘protected’ by formalin and mixed in the following proportions, 100:0; 66:34; 34:66; and 0:100 respectively, in the four concentrates. The daily intakes of silage DM were not significantly different on the four treatments and averaged 90 kg DM per cow, giving a mean total daily DM intake of 32.4 g kg−1 live weight. The milk yields were not significantly different on the four treatments and averaged 23.9 kg −1 The treatments had small and non-significant effects on milk composition and live weight. It is concluded that with a high-digestibility, well-preserved grass silage of satisfactory protein content the inclusion of ‘protected’ protein in the supplementary concentrate had no beneficial effects on milk production.

Notes: Small plots of permanent pasture containing 50%Agrostis capillaris-Festuca rubra were defoliated frequently or infrequently during the growing seasons in 1978–80 and fertilized with 133, 125 and 125 kg N, P2O5 and K2O respectively ha−1 a −1 or not fertilized during 1978–81. Yields of herbage were assessed during 1979–80 and botanical composition assessed on three occasions in 1978, once in April 1979 and once in 1982.Fertilizer application increased annual dry matter (DM) harvested from frequently cut plots by 40%, 53% and 65% in 1978, 1979 and 1980 respectively, and on infrequently cut plots it doubled hay DM harvested in 1978 and increased total DM harvested by 126% and 186% in 1979 and 1980 respectively. Infrequent cutting and fertilizer both decreased the proportion of A. capillaris but only fertilizer decreased the proportion of F. rubra. Fertilizer greatly increased the proportion of Holcus lanatus especially where plots had been cut frequently and of Alopecurus pratensis where they had been cut infrequently. It decreased the proportion of Luzula campestris. Infrequent cutting, especially with fertilizer, discouraged Cerastium fontanum ssp. glabrescens and Trifolium repens but encouraged Rumex acetosa.

Notes: Data on sheep live weights, herbage standing crop and herbage quality are presented from a 3-year grazing management study comparing set stocking, four-paddock rotational grazing and four-paddock forward rotational grazing systems. In both rotational grazing and forward rotational grazing systems, sheep were sequentially moved from one paddock to the next every 5 days. Lambs were weaned at 12–15 weeks of age in the forward rotationally grazed system and from then on grazed one paddock ahead of the ewes. Herbage mass was consistently greater on the rotationally grazed system than on the set-stocked system, averaging 38.4% more herbage in 1978. 32.8% more in 1979 and 52.7% more in 1980. No differences were observed in ewe live weights at the end of the grazing year between the rotationally grazed and the set stocked systems. Live weights of rotationally grazed lambs were superior to set-stocked lambs only when herbage allowance was low; otherwise no difference existed between these two systems. The forward rotationally grazed lambs generally had lower liveweights than did the rotationally grazed lambs, perhaps due to weaning stress.

Notes: Comparisons between some aspects of grazing and ruminating behaviour by sheep recorded automatically and by manual observation are reported.Behaviour was recorded manually and automatically on four swards with surface heights of 3, 6, 9 and 12 cm. The automatic recording method gave significantly higher biting rates (78 v 67 ± 2·4 bites min−1) than manual recording. Possible reasons for these differences are discussed. For recordings during ruminating there was no significant difference between the two recording methods.As sward surface height increased biting rate decreased by 4·6 (± 0.65) and 2·9 (± 0·87) bites min−1 cm−1, and masticating rate increased by 4·5 (± 1·13) and 3·7 (± 0·95) chews min−1 cm−1 for the manual and automatic recording methods respectively.It is concluded that the automatic recording system gives higher rates for jaw movements during grazing than manual recording but there is little difference between the two methods for activities recorded during ruminating.

Notes: An experiment was carried out in which simulated swards of ryegrass (cv. S23) were grown in boxes. In the first instance the swards were cut at weekly intervals to maintain five levels of leaf area index (LAI) from LAI 1 to 4–5 in simulation of continuous grazing. Measurements were made of growth, senescence and net growth rate and of net canopy photosynthesis at constant irradiance. The results showed that the swards adapted to the defoliation regimes mainly by changes in tiller population density and pseudostem length. When the swards had equilibrated to the cutting regime growth rate increased with LAI but, since tiller density and the partitioning of growth between herbage harvested and that lost by sensecence also changed with LAI, net growth rate was constant over the LAI range 2–4·5. Maximum weight of herbage harvested was obtained between LAI 2 and 3.After 10 weeks of weekly cutting all the swards were cut back to LAI 1 and allowed to regrow. Growth rate showed almost no response to the previous culling treatments. The relationship of net canopy photosynthesis to LAI was linear for the frequently defoliated swards and curvilinear for regrowing swards. The reasons for this difference were examined.

Notes: White clover seedlings were defoliated when either two, four or six leaves had opened. There were four defoliation treatments, involving removal of younger laminae, older laminae, all laminae or all laminae and petioles, plus a control (no defoliation).Leaf removal reduced area of subsequently emerging leaves when measured as they became fully opened, though some compensatory expansion occurred after this. Petiole length was also reduced considerably. The magnitude of these effects varied according to severity of the treatment: removal or older laminae had little effect, removal of younger laminae had a similar effect to removing all laminae, but the effect was considerably increased by removing petioles in addition to laminae. The youngest plants showed the greatest reductions in leaf size following leaf removal. Defoliation had little effect on the rate of development of subsequent leaves.The most severe defoliation treatments reduced plant dry matter but younger seedlings appeared to have a capacity for recovery equal to or greater than that of older seedlings.It is concluded that white clover seedlings have a considerable ability to recover from leaf removal, especially if only laminae of old leaves are removed, but the growth reduction following removal of petioles as well as laminae appears to be especially severe.

Notes: Experiments were carried out over three seasons in which swedes were sown by precision drilling after ploughing and conventional cultivation or by direct drilling with two types of drill at two row widths with and without shallow cultivation. The swedes were sown in June or early July following a first silage cut and sward desiccation with paraquat. Precision drilling with inter-row cultivation and singling gave a uniform weed-free stand and had the highest mean yield of over 8 t DM ha−1 Where hoeing was omitted yields were reduced due to weed competition. Yields following direct drilling were lower than with precision drilling due to uneven plant establishment, weed competition and grass regrowth. A shallow rotavation prior to direct drilling improved uniformity and yields and reduced grass regrowth. Direct drilling produced high numbers of small roots. The highest root yields were associated with sowing in early June and harvesting in December or January. Overall yields from the silage cut plus direct drilled swedes and silage cut plus precision drilled swedes were 12 and 13 t DM ha−1respectively compared with about 14 t DM ha−1expected from a grass sward.

Notes: Botanical composition of swards can markedly affect their productivity, forage quality, and acceptance by animals. The objective of this research was to determine the amounts of dry matter (DM) and N harvested in the different botanical components of swards where perennial ryegrass and orchardgrass were sown alone and fertilized with N, or sown in mixture with legumes.Broadcast sowings were made on conventionally prepared seedbeds in August 1979 and May 1980 on a Hagerstown silt loam soil (fine, mixed mesic Typic Hapludalf). When seeded alone, orchardgrass cv. Pennlate and perennial ryegrass cv. Reveille received rates of N ranging from 0 to 448 kg ha−1 a−1. The grasses were also grown in mixtures with alfalfa cv. Arc, red clover cv. Arlington, or birdsfoot trefoil cv. Viking. The same legumes were sown alone. Four legume seed rates were used in both the pure legume and mixed stands.Legume DM harvests were greater in mixtures with perennial ryegrass than in legume-orchard-grass swards. An exception occurred in the first year as birdsfoot trefoil harvested DM was lower in trefoil-ryegrass swards than in trefoil orchardgrass mixtures, probably owing to vigorous competition from ryegrass in the seedling stage. As legume seed rates were increased, both the legume and grass components of mixed swards produced more DM and N. Grass DM and N harvested in orchardgrass mixtures exceeded those of ryegrass mixtures for the 3-year period.Sward weediness, most prevalent in pure legume stands planted at low seed rates, was sharply reduced by including either grass with the legumes. Volunteer legumes contributed to DM harvested only when the grasses were sown alone and not fertilized with N.Species differences in crude protein (CP) concentration were significant. Birdsfoot trefoil had the highest CP of the legumes, averaging 241 g (kg DM)−1. Alfalfa and red clover averaged 219 and 208 g CP (kg DM)−1 respectively. Perennial ryegrass herbage had a higher CP concentration than that of orchardgrass in both pure and mixed swards. Grass CP increased significantly in response to fertilizer N and to higher legume seed rates.

Notes: Four cocksfoot varieties were harvested at three different growth stages and offered ad libitum to yearling Clun wethers in three 24-d feeding trials indoors over a 4-month period. A further cocksfoot variety conserved in bulk was offered concurrently as a control feed.The concentration of digestible organic matter in the dry matter (DOMD) and voluntary intake were measured in each feeding period. There was no significant change in the measured DOMD values between the different feeding periods. Voluntary intake of digestible organic matter (DOMI) increased over the 4-month period for all four varieties and for the control feed. The increase in intake was greatest for the most digestible material, i.e. first-cut harvests. Differences in DOMI were statistically significant between feeding periods for all varieties but the differences could be eliminated by correcting intakes with values from the control feed. It is concluded that comparisons of grass varieties involving intake measurements must either be made in the same feeding period or a control feed included and results corrected according to the control response.

Notes: Records of grassland productivity were kept for 2 years on 136 lowland dairy farms and 131 lowland beef farms. The grassland on each farm was classified according to the proportion of sown (preferred) species. The farms were then ranked on this basis and divided into quarters:On dairy farms the lowest quarter had 31% sown species and utilized metabolizable energy (UME) output from grass of 40.8 GJ ha−1, whereas the highest quarter had 70% sown species and UME output of 45.6 GJ ha−1. Dairy farmers in the lowest quarter used least fertilizer N and differences in output were no greater than would be expected from differences in N input. On beef farms the lowest quarter had 28% sown species and UME output of 32.3 GJ ha−1, and the highest quarter had 63% sown species and UME output of 45.9 GJ ha−1On these farms differences in fertilizer N could not completely account for differences in output.It is concluded that on beef farms botanical composition of grassland is a good indicator of its productivity but is not necessarily a prime cause of differences in output. On dairy farms there is little evidence of a direct benefit resulting from a high proportion of sown species. On such farms the need for deliberate manipulation of the botanical composition of grassland by reseeding or chemical means is questioned.

Notes: Twenty-one strains of lactic acid bacteria were subjected to a range of tests based on established criteria to ascertain their suitability for use as inoculants for silage. The tests included a monitor of their growth in the presence of representatives of the microflora of forage crops with which they might have to compete for substrate, and their ability to effect rapid acidification and achieve a low pH in conditions which simulate low and high ensiling potential. In addition, their ability to produce acid from sugars and their action against organic acids found in forage crops, together with their tolerance of acid, elevated temperatures and conditions of low moisture availability (e.g. in wilted material) and possible proteolytic and amylolytic activities were examined. None or these cultures satisfied all of these criteria. However, three strains (Streptococcus durans str. 1024, Lactobacillus acidophilus, str. 2356 and Lactobacillus plantarum str. 6) had a greater potential than the others.

Notes: Three grass silages made in sunny weather in early July from second-harvest perennial ryegrass were compared in a 16-week feeding experiment with twelve Ayrshire cows. The silages were either unwilted or wilted with and without conditioning, and had mean dry matter (DM) concentrations of 201, 261 and 272 g kg−1, and in vitro DOMD concentrations of 650, 669 and 672 g kg−1 DM respectively. All the silages had formic acid (‘Add-F’) applied at a rate of 2.6 litres t−1 and were offered ad libitum plus 6 kg concentrates per cow per d. The daily intakes of silage DM were 905 kg per cow on the unwilted treatment and 9.86 and 9.65 kg on the wilted treatments with and without conditioning respectively. Daily milk yields were 171, 17.6 and 17.4 kg per cow on the unwilted, and wilted with and without conditioning treatments respectively and were not significantly different. Fat concentrations in the milk were not affected significantly by treatment, whereas the crude protein and solids-not-fat concentrations were significantly higher on the wilted than on the unwilted treatment. The efficiency of utilization of metabolizable energy for lactation was 6–7% lower with the wilted than with the unwilted silages and it is concluded that the unwilted silage was superior to the wilted silages as a feed for dairy cows.

Notes: Three diploid—Sabtoron, Violetta and Essex—and three tetraploid—Hungaropoly, Teroba and Red Head—red clover cultivars were sown alone and with each of three companion grasses—timothy (S48), tall fescue (S170) and perennial ryegrass (S24). The productivity and persistence of the red clover cultivars were compared. Results obtained over the first four harvest years were presented in an earlier paper (McBratney, 1981) and this paper presents results from the fifth and sixth (final) harvest years.The low yield obtained in the fifth and sixth year from Essex under each seeding treatment indicated a deterioration in its persistency or vigour or both. In the fifth year the remaining two diploids, Sabtoron and Violetta, were as highly productive and persistent as the three tetraploids but in the sixth year the decrease in productivity was greater in the diploids than in the tetraploids. In the fifth year Teroba, the highest yielder, produced 9 8 t total DM ha−1 over all seeding treatments of which 73% was clover DM; in the sixth year Teroba was again highest yielding, producing 9.3 t ha−l with 67% clover content. Tall fescue was the most consistent companion grass in the mixed swards, in the sixth year contributing 53% grass DM to the total 8 8 t DM harvested ha−1.The trial demonstrates the potential of a number of red clover cultivars grown either pure or with a suitable perennial grass to achieve and maintain a high output of herbage of good quality over a period of 6 years without any application of fertilizer N. The practical agricultural implications of using red clover are discussed.

Notes: Leaf sheaths of two cultivars of perennial ryegrass were prepared by chemical oxidation so that the silica bodies in the tissues could be studied. The cultivar Fortis, which is relatively resistant to stem-borer attack, contained many-intercostal silica bodies in the leaf sheath but the more susceptible cultivar S24 contained few. If the pattern of silica bodies affects levels of stem-borer infestation, it may be possible to breed for this characteristic.

Notes: Ammonia and sodium hydroxide (NaOH) have been used to improve the dry matter (DM) digestibility of roughages or to conserve them. These treatments have had incidental effects on pH alone or both the pH and nitrogen contents of the roughages. in the present study, the effects of pH and NH4OH on the in vitro dry-matter digestibility (IVDMD) of eight roughages—four legumes and four grasses—were investigated. The pH levels of approximately 5, 7, 9 and 12 were achieved by adding appropriate amounts of NH4OH or NaOH and H2SO4. Nitrogen levels of approximately 20, 30, 40 and 50 g kg−1 on a DM basis were obtained by adding NH4OH. Results of a multiple regression showed that increasing the pH caused a linear increment in IVDMD and that the added NH4OH reduced the increment of IVDMD. The effects of both pH and N on IVDMD were found to be specific to the individual roughage; the response of grasses to both pH and NH3 was greater than that of legumes

Notes: In view of the revived interest in sainfoin, an economic assessment of the prospects for an expansion of its area in Britain has been attempted. It is estimated that potentially it could be grown on 950 × 103 ha of the agricultural area of England and Wales, though it is unlikely in the near future that it would exceed 20 × 103 ha, even if economic conditions were favourable. Comparison of the energy and protein yields of sainfoin, red clover and lucerne under conservation management indicates that one of the attractions of sainfoin is its high protein content. However, an assessment of the costs of production and utilization of sainfoin indicate that, for it to be a more widely used legume, sustainable dry matter (DM) yields would need to be increased by 35% to about 11.5 × 103 kg DM ha−1. At the same time, although growing sainfoin as a mixture with grass appears to improve the economic attractiveness of the crop, silage production costs (kg DM)−1 are still about 15% lower on pure grass swards. However, trials in the UK, Canada and Rumania have shown that, compared to current UK levels, sainfoin yields can be significantly increased.

Notes: The relationship between net canopy photosynthesis (Pnc) measured at 400 J m−2 s-1 and leaf area index (LAI) was determined on ryegrass-dominant swards over a range of her bage masses in five grazing experiments. In three experiments the swards were continuously stocked either by sheep or by cattle to maintain a number of herbage heights and hence LAI values. In two experiments the swards were intermittently grazed with 21 -d regrowth intervals. On the continuously stocked swards measurements were made over nine periods each of 7 to 21 d in length. On the intermittently grazed swards measurements were made over six periods while the herbage was being grazed down from a high to a low LAI and over three periods during the regrowth phase.On all the swards where measurements were made while grazing was in progress, the relation of Pnc to LAI was linear. The photosynthetic rate was greater on the continuously grazed than on the intermittently grazed swards up to LAI 35 and in two experiments to LAI 5. The relationship of Pnc to LAI of swards in the regrowth phase was predominantly curvilinear and such that over much of the LAI range they had a greater photosynthetic potential than the same swards being grazed down from high to low mass. Daily rates of CO2 uptake and growth in the field were calculated for one continuously stocked experiment. Both were linearly related to LAI in the range LAI 2–4·5. The agronomic implications of the results are discussed.

Notes: An exact expression is derived for the optimal spacing between interfering wells in a rectangular well field in an ideal confined aquifer. A simple, practical method for determining the optimal spacing is presented. The optimal spacing is shown to be substantially different from the spacing determined by use of the Theis formulation. The economic savings resulting from use of the revised approach are evaluated and found to be considerable, especially when the number of wells is large and the transmissivity of the aquifer low.

Notes: Abstract. A hydrologic test system has been designed to measure the intrinsic permeabilities of individual fractures in crystalline rock. This report contains the design schematics and specifications necessary to build and/or modify this test system for other similar field applications. This system is used to conduct constant pressure/declining flow rate and pressure pulse hydraulic tests. The system is composed of four distinct units: (1) The Packer System, (2) Injection System, (3) Collection System, and (4) Electronic Data Acquisition System. The apparatus is built in modules so it can be easily transported and reassembled. It is also designed to operate over a wide range of pressures (0–300 psig) and flow rates (0.2–1.0 gal/min). This system has proved extremely effective and versatile in its use at the Climax Facility, Nevada Test Site.

Notes: Field and laboratory analyses of pH, alkalinity, and specific conductance from water samples collected from the Columbia aquifer on the Delmarva Peninsula in eastern Maryland were compared to determine if laboratory analyses could be used for making regional water-quality interpretations.On the basis of 170 field pH and specific conductance measurements, 165 laboratory pH and specific conductance measurements, and 86 field and 135 laboratory alkalinity measurements, a significant difference at the 0.1-percent level was found between laboratory and field analyses of pH and specific conductance. No significant difference was found between laboratory and field analyses of alkalinity. In most samples, laboratory pH was greater than field pH, laboratory specific conductance was less than field specific conductance, and laboratory alkalinity was equally likely to be less than, the same as, or greater than field alkalinity.Kruskal-Wallis tests of field and laboratory data grouped by north-south and east-west coordinates and by land use indicate that the difference between field and laboratory values is usually not enough to affect the outcome of the statistical tests. Thus, laboratory measurements of these constituents may be adequate for making certain regional water-quality interpretations, although they may result in errors if used for geochemical interpretations.

Notes: Preliminary vadose zone nitrate extraction experiments have revealed the extractant (2N KCl) volume affects the determination of nitrate and its nitrogen isotope ratio.In five cores, extractable NO3-N concentrations increased an average of 1.7 times after the soil-to-extractant ratio was increased from 1:1 to 1:10. An increased extractant volume resulted in a large positive shift of stable nitrogen values (δ15N), which averaged +6.2%.An underestimation of available NO3-N for leaching and transport through the vadose zone and a biased source interpretation from the δ15N values probably would occur if a procedure which leads to incomplete extraction of nitrate is used.

Notes: Currently, one of the most popular methods of containing contaminated ground water is through use of subsurface impermeable barriers. These barriers can take one of three forms: slurry walls, grout curtains, or steel sheet piles. Successful operation of these barrier systems is dependent upon three basic criteria. First, the barrier must be truly impermeable and remain so over time even upon exposure to the contaminated ground water. Second, there must exist an underlying impermeable formation, at a reasonable depth, to which the barrier can be connected. Third, an adequate connection between the barrier and the underlying formation must be assured.This paper presents the results of the analysis of the movement of contaminated ground water under or through an imperfect barrier. The first phase of the analysis consists of the development of an analytical solution for the flow of ground water under a barrier and a simple numerical integration technique for developing concentration breakthrough curves. This simple solution algorithm was applied to the cases of variable recharge rates and lengths, variable depths of penetration of the barrier, and anisotropic soils. The second phase of the analysis involves applying a numerical solute transport model to analyze the performance of a barrier with and without the effects of hydrodynamic dispersion, and in the presence of a layered soil, and finally the performance of a fully penetrating but partially permeable barrier.

Notes: Hydrogeologic and ground water quality data obtained from a gas-driven multilevel sampler system and a polyvinyl chloride (PVC) monitoring well nest with the same aquifer communication intervals are compared. All monitoring points are in close proximity to each other.The study was conducted at an eight-acre uncontrolled hazardous waste site. The site is located in an alluvial valley composed of approximately 40 feet of alluvium overlying shale bedrock. The ground water at the site is contaminated with various organic constituents.A ground water monitoring network consisting of 26 conventional monitoring wells, nine observation well points, and six multilevel gas-driven samplers was established to characterize the hydrogeologic regime and define the vertical and horizontal extent of contamination in the vicinity of the abandoned chemical plant. As part of this study, a multilevel monitoring system was installed adjacent to a well nest. The communication zones of the multilevel samplers were placed at the same elevation as the sand packs of the well nest. The multilevel sampler system and well nest are located in a contaminated area directly downgradient of the site. A comparison of the vertical head distribution and ground water quality was performed between the well nest and the multilevel sampling system.The gas-driven multilevel sampling system consists of three gas-driven samplers that monitor separate intervals in the unconsolidated materials. The well nest, composed of two PVC monitoring wells in separate boreholes, has the same communication interval as the other two gas-driven samplers.Hydraulic head information for each multilevel sampler was obtained using capillary tubing. This was compared with heads obtained from the well nest utilizing an electric water level indicator.Chemical analyses from the PVC and multilevel sampler wells were performed and compared with one another. The analyses included organic acids, base neutrals, pesticides, PCBs, metals, volatile organics, TOX, TOC, CN, pH and specific conductance.

Notes: A total of four vertical electrical soundings were conducted in a layered andesitic rock aquifer known in places to yield ground water with total dissolved solids (TDS) in excess of 2,000 milligrams per liter (mg/L). The objective of the soundings was to locate zones of moderate to high permeability but with acceptable chemical quality.The resistivity of a geologic unit is a function that includes the quantity of total dissolved solids in the interstitial water and the distribution of the water within the unit. Thus, the resistivity of most granular soils and rocks is controlled more by porosity, water content and water quality than by the conductivity of the matrix materials.The electrical data delimited a drill site where it was believed that ground water of acceptable chemical quality could be expected. Completion and test pumping of two exploration wells confirmed the electrical sounding results.The first test well drilled prior to the survey yielded only small amounts of ground water with total dissolved solids in excess of 2,000 mg/L. The second exploration well drilled at the site as a result of the electrical study yielded in excess of 100 gallons per minute of ground water with total dissolved solids of 830 mg/L.

Notes: An advanced two-way radio link (transceiver units) and data logging system have provided a powerful means of real-time series data collection and analysis. The application of this telemetry system to surface tiltmeter surveys provides significant cost savings for testing and analysis of deep, large-scale horizontal anisotropic permeability and heterogeneity determinations.The determination of horizontal anisotropic properties requires one pumping well and eight to 10 tiltmeter monitoring stations. The validity of the depth of the test may reach 4,000 feet below land surface. With the addition of one partially penetrating observation well, the entire three-dimensional anisotropic permeability can be determined. With the addition of a fully penetrating well, the storage coefficient can be determined as well as leakage property (including vertical permeability of the confining layers). An example test case was analyzed for horizontal anisotropic permeability with remarkable success despite relatively unfavorable detection conditions.A thorough understanding of the geology overlying the aquifer system must be made in order to assess the applicability of the surface tiltmeter survey to large-scale horizontal anisotropic permeability determinations.

Notes: This paper describes a new system for ground water monitoring, “the BAT System,” which includes the following functions: (a) sampling of ground water in most types of soils, (b) measurement of pore water pressure, and (c) in situ measurement of hydraulic conductivity. The system can also be used for tracer tests. The system utilizes a permanently installed filter tip attached to a steel or PVC pipe. Installation is normally performed by pushing the tip down to the desired depth. The filter tip can also be buried beneath a landfill. The primary feature of the new system is that the filter tip contains a self-sealing quick coupling unit, which makes it possible to temporarily connect the filter tip to adapters for various functions, e.g. water sampling and for measurement of pore pressure and hydraulic conductivity. The new technique makes sampling of both pressurized water and gas possible. Samples are obtained directly in hermetically sealed, pre-sterilized sample cylinders. Sampling of ground water and measurement of pore pressure can be repeated over a long period of time with undiminished accuracy. This technique is also well-adapted for taking water samples from different strata in a soil profile, in both the saturated and unsaturated zones. Actual installations range from 0.5 to 60m depth.

Notes: State-of-the-art analytical techniques are capable of detecting contamination In the part per billion (ppb) range or lower. At these levels, a truly representative ground water sample Is essential to precisely evaluate ground water quality. The design specifications of a ground water monitoring system are critical in ensuring the collection of representative samples, particularly throughout the long-term monitoring period.The potential interfaces from commonly used synthetic well casings require a thorough assessment of site, hydrogeology and the geochemical properties of ground water. Once designed, the monitoring system must be installed following guidelines that ensure adequate seals to prevent contaminant migration during the installation process or at some time in the future. Additionally, maintaining the system so the wells are in hydraulic connection with the monitored zone as well as periodically Inspecting the physical integrity of the system can prolong the usefulness of the wells for ground water quality. When ground water quality data become suspect due to potential interferences from existing monitoring wells, an appropriate abandonment technique must be employed to adequately remove or destroy the well while completely sealing the borehole.The results of an inspection of a monitoring system comprised of six 4-inch diameter PVC monitoring wells at a hazardous well facility Indicated that the wells were improperly installed and in some cases provided a pathway for contamination. Subsequent down hole television inspections confirmed inaccuracies between construction logs and the existing system as well as identified defects in casing materials. An abandonment program was designed which destroyed the well casings in place while simultaneously providing a competent seal of the re-drilled borehole.

Notes: Transmissivity can be estimated by several well documented methods employing data from rising water level slug tests in wells. A very simple and relatively inexpensive system can be constructed to lower the water level in a well. Compressed air is injected through a sealed device called a well head manifold, which screws onto the casing top and contains an air pressure gauge, an air entry valve, a quick release valve and a multi-channel water level indicator or a pressure transducer. Either of the latter is lowered into the well prior to pressurization.Compressed air is injected into the casing at a low rate through the manifold, depressing the water level a desired amount. After stabilization, the quick release valve is opened and the air pressure inside the casing is reduced to atmospheric pressure instantaneously; the water level then starts to rise. Successive elevations of the rising water level are determined with the indicator or transducer and their elapsed times from valve opening are recorded. Plots of water level recovery vs. time can then be used to estimate transmissivity by the published methods of Cooper, Bredehoeft and Papadopulos (1967), Ferris and Knowles (1954) and Hvorslev(1951).All of the items used for construction, with the exception of the quick release valve, can be bought off the shelf. The valve can be easily constructed in a machine shop. The total cost of the device, exclusive of the transducer, should be less than $500.

Notes: Ground water flow in karst terranes generally occurs in the solution channels of carbonate aquifers. A hydrogeologist may utilize borehole geophysical methods to identify these solution channels in aquifers. Two specific methods that are applicable in karst terrains are:1. Natural gamma ray logging2. Borehole caliper logging.Gamma ray logging can detect the presence of inter-bedded strata in the main limestone unit, such as shale, which emit high levels of gamma radiation. Gamma ray logging can also detect clay deposits in solution channels that may act to restrict the flow of ground water. The areal extent of these rock strata or clay-filled solution channels can be determined when gamma ray logs are conducted at several borehole locations across the site of investigation.Borehole caliper logging can be employed to determine the presences of solution channels within the aquifer when penetrated by a borehole. In addition, since shale layers and clay filling are less resistant than the surrounding limestone, the caliper log may detect both the presence and the thickness of shale or clay layers in the aquifer.Gamma ray logs can be used in conjunction with caliper logs to provide data on the stratigraphic location and thickness of solution channels and clay and shale layers within a limestone aquifer. This information is valuable to the hydrogeologist performing investigations at sites located in limestone terranes because ground water flow preferentially occurs along solution channels.

Notes: Column leaching experiments show that fly ash Interlayered with sulfide-rich tailings can reduce the vertical permeability of tailings by at least three orders of magnitude. Chemical reactions between the fluid, alkaline fly ash from Western coal and pyritic tailings result in a one to three order of magnitude reduction in the dissolved metals content of the effluent solution. Metal precipitation, which produces the dissolved metals reduction, is a significant element in the permeability reduction. The combined factors reduce the metals flux from the tailings by four to six orders of magnitude. We believe that tailings ponds with fly ash layers as amendments can greatiy reduce ground water contamination from pyritic tailings. In addition, concentrated application of fly ash layers near the end of mining should act to restrict infiltration and access of oxygen, thereby enhancing reclamation. Similar results maybe obtainable using other amendments with lower base capacity (lime, crushed limestone, trona).

Notes: This article describes a computer automated, hydrologic analysis system designed to allow the collection of high quality, long-term pumping test data. The instrument solves two of the major problems encountered in the field during aquifer tests: insufficient data, particularly during the early part of a test when drawdown is rapid; and high labor costs associated with long-term monitoring.To illustrate the system's application, results are presented from the test of a highly transmissive aquifer. The aquifer's drawdown response was rapid; thus the time-drawdown curve was essentially flat after the first two minutes of the test, and correspondingly rapid data acquisition was essential for a unique solution of the aquifer's three-dimensional hydraulic conductivities.

Notes: Traditional monitoring methods using chemical analysis of ground water samples to detect pollutant migration are being superseded or used in conjunction with innovative approaches. A need to detect pollutants before they reach the water table has drawn interest to vadose (unsaturated) zone monitoring and brought together hydrogeologists, soil scientists and agricultural engineers who have been working on this subject for years.Recent studies have identified over 50 different types of vadose zone monitoring devices and methods that have optimum utility in varying hydrogeologic settings. In general, measurements made in the vadose zone are trying to define storage, transmission of liquid waste in terms of flux and velocity, and pollutant mobility.Criteria for the selection of alternative vadose zone monitoring methods are important for the development of site-specific systems. These criteria include: type of site; applicability to new, active, and abandoned sites; power requirements; depth limitations; multiple use capability; type of data collection system; reliability and life expectancy; degree of operational complexity; direct versus indirect methods; applicability to alternate media; effect on flow regime; and effect of hazardous waste on sampling or measurements. Application of the selection criteria is discussed in Everett et al. (1982a).

Notes: In recent years we have seen major advances in the variety and quality of ground water sampling instrumentation, in almost every case involving increases in sensitivity of one or more orders of magnitude. Software number-crunching ability brings us the capability of running sophisticated mathematical and statistical programs, while modern analytical chemistry routinely makes determinations of metals, organics and radioactive elements accurate in the parts per billion range.The construction of sample points rarely supports these levels of precision. The well is the basic sampling machine for the ground water industry and its ability to produce a truly representative sample underpins all data and conclusions. Regardless of the type of instrumentation employed, a sample point that cannot be shown to be truly representative and that fails to provide a series of reliable samples over the long term–let us say 25 years minimum–is a negative investment.In the authors’professional sampling work, it has been observed that, wherever it is possible to improve the quality of the ground water sample point, a reduction in the measured level of pollutant elements in the sample is found and the confidence level improves. Volatile organic values are the sole exception to this observed reduction in concentration, the reason being that sample point and method improvements capture the fully representative water sample, thus reducing volatile losses.It is not possible to address all hydrogeologic environments and sampling methods in this review. Instead, features were selected that had been found to be important in commercial, high-precision, water quality sampling in Western U.S. environments. In this area, water movement is slow and little or no exchange of water occurs between wells and the surrounding aquifer without artificial stimulation. Water quality stabilization requires multiple casing volume evacuations in wells ranging to 1,000 feet deep. High-volume pumping or mechanized bailing is an economic requirement. Dedicated installations cannot supply the volume of water required for comprehensive analytical programs.It is the nature of these sources, as well as practical recommendations for corrective action, that will be addressed in this article.Any attempted improvement in utilization of data by mathematical processing or statistical treatment will be inadequate because:〈list xml:id="l1" style="custom"〉• Samplings are infrequent and often accumulate at rates of only two or four sample suites per year. Seasonal variations may obscure evidence of passage of a low-intensity pollution front and some years may elapse before the true situation becomes apparent from statistical analyses. By such time, the problem pollutant may have escaped from the treatment area.• Powerful biases occur due to the failure of the sample point to eliminate false contributions to the sample from sources other than the natural environment or a pollution source, most frequently originating in casing materials and annular seal leakage.

Notes: At present, there is no direct evidence of rocks predating the late Paleocene opening of the Norwegian-Greenland Sea on the Jan Mayen Ridge. A review of the available geophysical data, DSDP drilling results and plate tectonic reconstructions convincingly indicates a continental nature of the northern part of the ridge. On the other hand, there is still considerable uncertainty about the southern part of the ridge and its possible continuation towards Iceland. Two reflectors, A and O, have been mapped regionally. A appears to reflect an unconformity of middle Oligocene age. Most investigators have indicated that O forms a late Paleocene rift unconformity associated with the opening of the Norwegian-Greenland Sea. By analogy with the North Sea and the continental margin off Norway we propose that it should be investigated whether this reflector might be older, relating to an earlier Mesozoic regime of tension.

Notes: Finds of pumice on raised beaches in the inner Isfjorden area are reported. Pumice is abundant in two zones, and four levels can be distinguished in some areas. The highest lying level has the greatest concentration of pumice and is dated to a maximum of 6, 500 years B.P. Tentative correlations with pumice levels from other places in Svalbard indicate approximate ages of 6, 000, 4, 100, and 3, 100 years for the lower levels in inner Isfjorden. A shoreline displacement curve based on the pumice levels and on 10, 000 year old driftwood is presented.

Notes: Aristoptychites kolymensis (Kiparisova) is investigated and described in detail from collections made at Botneheia, Spitsbergen, where it occurs at the top of the Botneheia Formation (Daonella Shale). It is a rather small species and the stratigraphically highest representative of the genus in Spitsbergen. Its suture line is remarkable by its possession of the additional U.-lobes between the internal lobe and the first umbilical lobe. A lobe of this kind has so far been described only once, with Arcestes (Proarcestes) bicarinatus by Schindewolf (1968), which suggests affinities between Ptychitidae and Arcestidae.

Notes: Phosphine gas (PH3) is a widely used fumigant for stored fruits and grains. Previous studies indicated that all residues of this gas are eliminated by normal aeration but the exact time has never been determined.The objective of this work was to determine the time required for desorption of phosphine from fumigated dates relative to aeration time and temperature. Flame photometric detection by gas chromatography was used for the detection of sorbed phosphine.Results indicate that the initial residue level of PH3 falls rapidly within 24 h after fumigation but residues persist for at least nine days. Higher residue levels were found in dates stored at low temperatures (4°C) than at 28°C. It is recommend that fumigated dates be marketed after longer periods of aeration, especially those aerated at temperatures of 4°C or less.

Notes: Staphylocoagulase is highly heat resistant. Inactivation profiles of crude staphylocoagulase at 80, 100 and 121°C showed that total inactivation occurred after heating for 5 h,2 h and 30 min, respectively. Heat treated coagulase has the ability to reactivate when placed at 25°C for 24 hrs similar to previous observations on staphylococcal enterotoxins B and C. Staphylocoagulase could be recovered from beef broth and chicken broth before or after heating at 80°C for 5 min. Coagulase activities were measured by a quantitative capillary tube method.

Notes: A sterile agar-meat was acidified with lactic acid and seeded with Staphylococcus aureus. Monolaurin (Lauricidin®), which is a GRAS emulsifier, caused both static and cidal effects on S. aureus 196E. When compared to potassium sorbate, Lauricidin® was slightly more active. The combination of sorbic acid and Lauricidin® (Lauribic™) gave a greater inhibitory effect than either of the substances alone. Inhibitory effects were greater on “anaerobic” than “aerobic” growth. These results indicate that monoglyceride emulsifiers like Lauricidin® alone or in combinations with other preservatives can be used in foods to create environments which are hostile to microorganisms.

Notes: Even advanced countries suffer from infectious diseases spread by food animal origin. The underlying mechanism of this morbidity is known and is important to design measures to reduce the incidence via intervention sensu Wilson (1955).The classical defense lines which rely on high-level hygiene in the slaughter-house and during food preparation are insufficient to control these food transmitted enteric infections. Consequently, a third line of defense, processing for safety, is required here as it was in the dairy and the egg product industries. At least two effective modes of processing for safety of raw food animal origin are available to erect this third line of defense. These includ radicidation and lactic acid decontamination. The two procedures lead to marked reward in terms of control of infection, while they are both fully safe.It is the duty of Public Health bacteriologists to advise regulatory agenci and consumers in applying one or both of these measures for improved healt protection.The health profession cannot possibly hope to achieve this goal without recruiting the assistance of experts in human behavior and more particular in factors determining selection and rejection of foods by consumers.

Notes: Conjugation of sterigmatocystin (ST) to bovine serum albumin (BSA) was achieved by converting ST to its hemiacetal followed by a reductive alkylation method under conditions similar to those for the preparation of afla B2a-BSA conjugate. Useful antibody was obtained from rabbits 6 weeks after they were immunized with BSA-ST conjugate. Antibody titers reached maxima (1:51,200) 10–12 weeks after immunization. ELISA analysis revealed that the dehydro-ST is about 16 times less reactive with the antibody than is ST. The antibody practically has no cross reaction with aflatoxins B1, B2a, G1, G2, and M1, and 6-0-methyl-ST.

Notes: The antimicrobial activities of Lauricidin (glycerol monolaurate) and its formulations Lauricidin Plus F, and Lauricidin 812 were neutralized by Tweens 20 and 80. The quantitative relationship depended on the formulation of Lauricidin, the test organism and the concentration of the Tweens. In general Tween 20 and Tween 80 were similar in their abilities to neutralize the antimicrobial properties of Lauricidin. In most cases, (74% of the observations) 3.0% Tweens completely neutralized Lauricidin and its formulations.The effects of the Tweens on Lauricidin and Lauricidin Plus F were also studied quantitatively. In the presence of Lauricidin and Tweens, no inhibition was noticed and in most cases, the viable count was similar to the control. Lauricidin Plus F was much more potent than Lauricidin in inhibiting the organisms tested and the restoration of growth in the presence of the Tweens was not complete. The viable counts in the presence of Lauricidin Plus F and the Tweens were about two or more log cycles lower than the control.

Notes: Salt or sodium chloride (NaCl) is a common ingredient in many processed foods, and especially in cured meats. In addition to flavoring and functional contributions, NaCl is believed to play an important antimicrobial role in these products. The antimicrobial activity of NaCl is reviewed in light of currrent calls for a reduction of Na+ in the human diet due to health reasons, and the possible replacement of NaCl in processed foods with chloride salts of other ions (i.e. KCl, MgCl2, CaCl2). Factors interacting with NaCl and complicating the nature of the preservative system in processed foods are examined; recent work with alternative chloride salts is summarized; complex solute-water activity interactions are highlighted; and, several important factors are considered in relation to the antimicrobial effects of NaCl and the possibility of its replacement with other chloride salts.

Notes: The dissected till plains physiographic province contains extensive areas of pre-Illinoian drift in Kansas, Nebraska, Iowa, and Missouri. The drift is typically a clay-rich, oxidized, and highly weathered till. In many areas of Missouri, this shallow drift represents the only readily available aquifer suitable for domestic and small agricultural users. There are, however, only few published data on the region's hydrogeology. Water budget analyses indicate that approximately 30% of mean annual rainfall is discharged by stream flow and 70% by evapotranspiration. The shallow drift flow systems are best considered unconfined, steady-state systems. Each watershed corresponds roughly to a local ground-water system. There is, typically, little hydraulic connection between the shallow drift systems and underlying strata. The drift hydraulic conductivity varies from approximately 1011 m/sec (laboratory tests) to 10−6 to 10−7 m/sec (field tests) when permeable sand lenses or joints are intersected. Ground-water discharge is concentrated in the immediate vicinity of stream channels, and recharge is concentrated on nearly horizontal hillslope summits. The ground water is generally potable (averaging 455 mg/1 total dissolved solids), but may be high in iron or polluted locally by organic wastes.

Notes: Hydraulic properties of saturated and unsaturated stony soils were studied on a 3.35 m long column, 1.24 m in diameter, filled with alternating sand and boulder layers. The boulders averaged 6.2 × 15 × 20 cm in size and were laid down on their flat side. Tensiometers and a neutron probe access tube were placed in the column for measuring pressure heads and water contents, respectively. Saturated conditions were obtained by ponding the column. The resulting hydraulic conductivity K was 5.1 m/day. This value could also be calculated from the measured K for the sand alone on separate samples, using a simple equation that takes into account the void ratio of the sand alone and that of the boulder-sand mixture. Unsaturated K was determined by applying water at less than ponded infiltration rates. Resulting relations between the unsaturated K and water content or negative pressure head could also be estimated from the relation between unsaturated K and pressure head for the sand alone and the calculated saturated K of the boulder-sand mixture. The method of Millington and Quirk for calculating the relation between unsaturated K and water content also gave reasonable results. The dispersivity of the boulder-sand column was 18 times that of the sand alone. Pore velocity was accurately estimated as the Darcy velocity divided by the volumetric water content. Hydraulic properties of stony vadose zones are difficult to determine. This work shows that they can be estimated from K relations measured in the laboratory on samples of the soil between the rocks. Knowledge of hydraulic properties of vadose zones is important in predicting movement of water and pollutants to the underlying ground water.

Notes: Three case studies from New Mexico illustrate methods by which aquifers can be classified for purposes of the Federal Underground Injection Control program. The principal technique involves preparation of hydrogeologic maps or cross sections which display information on the permeability of rock units and the dissolved solids content of formation fluids. Because deep water wells are lacking in most areas, the analysis normally requires considerable interpretation of geological and geophysical logs collected by energy and mineral companies, plus use of a general model or concept about regional hydrogeology. Injection of waste fluids into aquifers containing water with less than 10,000 mg/1 dissolved solids is not allowed unless an exemption is justified by economic, engineering and other factors. Based on the case studies, regulatory exemptions will be possible for aquifers which are hydrocarbon or mineral-producing, or which are important for brine disposal purposes.

Notes: A parameter-estimation technique based on existing hydrological, geophysical, and geological data was developed to approximate transmissivity values for use in a ground-water flow model of the Animas Valley, southwest New Mexico. Complete Bouguer gravity anomaly maps together with seismic-refraction profiles, geologic maps, geologic, geophysical, and drillers' logs, water levels, and pumping-test data provide insight into the transmissivity of bolson deposits throughout the basin.The transmissivity distribution was primarily based on reported pumping and specific-capacity tests in conjunction with complete Bouguer gravity anomaly maps and well log data. Reported transmissivity values were characterized by gravity values and well log data. In grid blocks lacking pumping and specific-capacity tests, transmissivity values were assigned based on the relationship of gravity values and well log data within the grid block to gravity values and well log data within other grid blocks for which transmissivity values are available.A two-dimensional, finite-difference, ground-water flow computer code was used to evaluate the effectiveness of the parameter-estimation technique. Although the trial-and-error method of calibration was employed, the actual computer time necessary for model calibration was minimal. The conceptually straightforward approach for parameter estimation utilizing existing hydrological, geophysical, and geological data provides realistic parameter estimates.

Notes: Most of the current simulation models for unconfined aquifers are based on the assumption that the free surface variation is small so that it can be combined with permeability to reduce the nonlinear Boussinesq equation to a linear partial differential equation (PDE). One of the most obvious reasons for using the linearization assumption is for the ease of numerical solution. This work presents a simpler alternative which permits an easy direct solution of the Boussinesq equation. A forward in time, central in space (FTCS) explicit finite difference method is used in the simulation model. The model was first validated by comparing its results with known analytical solution. It was then applied to an actual situation in which the short-term responses (from pumping) of an unconfined aquifer were simulated. The study shows that the stability of the model can be easily controlled, and because of the simple algorithm used, the code can be expeditiously developed and economically run on smaller machines. Due to the uncertainties in the calibration results, it is recommended here that more data be collected to improve the calibration before the model is used as a real-time simulation tool.

Notes: The investigation of the regional flow characteristics of aquifers is important with respect to aquifer management. To determine regional flows, use can be made of hydraulic and hydrochemical data. In a study of a Chalk Aquifer System in the south of Lincolnshire, England, a combined hydrochemical approach using major, minor and isotope chemistry is described. The chemical data have been used because hydraulic information is difficult to interpret both locally and regionally as a consequence of multi-layered hydraulic zones, nonhomogeneous fissure zones, and variable erosional features. By characterizing ground-water types on the basis of major ion chemistry, and subsequently interrelating with minor ion distributions (notably iodide supported by strontium), a reasonable assessment of operative flow paths and mechanisms has been made. Substantiation of the conclusions reached using major and minor ion chemistry is provided by the environmental isotope data of carbon and tritium.

Notes: Unlined municipal waste stabilization lagoons are potential sources of ground-water contamination. Fourteen monitoring wells were installed around the McVille, North Dakota lagoon, a site at which the impoundment is excavated into permeable sediments of an unconfined glacio-fluvial aquifer with a shallow water table. One cell at the site, Cell I, retains waste water continuously, while another, Cell II, is used for periodic overflow discharges from Cell I. Seepage through the bottom of Cell I passes through a strongly reducing organic sludge layer. Sulfate in the waste water is reduced to sulfide and possibly precipitated as sulfide minerals in or below this sludge layer. In the unsaturated or shallow saturated zone beneath the pond, the infiltrating waste water reduces ferric iron in iron oxide minerals to more soluble ferrous iron. Proximal down-gradient well analyses indicate high iron concentrations and very low sulfate levels. Downgradient wells near the lagoon have very high ammonium concentrations. The source of the ammonium is either rapid infiltration from Cell II or denitrification of the nitrate present in ground water upgradient from the lagoon. About 300 feet downgradient from Cell I, ammonium concentrations decline to near zero. The most likely mechanism for this decrease is cation

Notes: Abstract. Using known or estimated values of trans-missivity and storativity, the distribution of drawdowns at any time within a discretized flow field can be generated by applying simple trigonometry and numerical approximations of the exponential integral to the Theis equation. Single- and multiple-well systems, as well as image boundaries, are readily simulated with this method. A program employing this technique is presented in BASIC for use with microcomputers. The availability, low cost, and computational power of many small computers makes them ideal for this type of application. Their user-oriented features allow many possible combinations of wells, boundaries, and hydraulic properties to be analyzed in a short time.

Notes: A gravity survey was conducted in a 94-square-mile area of northeastern Hanson County, South Dakota. The 340 measured gravity values, together with test-hole data, were used to approximately delineate a buried valley eroded into the Sioux Quartzite of Precambrian age. This valley contains, in places, an aquifer composed of quartzose sand of pre-Cretaceous age derived from the Sioux Quartzite. The bottom of the valley is approximately 450–600 ft below land surface. Simple Bouguer values were determined from measured gravity data, referenced to a local base station, and interpolated to a 0.5- by 0.5-mile grid. The interpolated simple Bouguer values of residual gravity were determined using a five-ring, inverse-weighted filtering method. The second derivative of the interpolated gravity values, as well as their downward continuation, did not delineate the buried valley as well. Subsequent drilling of nine test holes showed that the gravity method can be used for approximately delineating subsurface features

Notes: Glacial buried-valley aquifers serve as primary sources of potable ground water in northeastern Kansas. A long known problem, however, is that a large percentage of well waters in this region exceed the U.S. Environmental Protection Agency (EPA) limits for nitrate (NO−3). A detailed study of the hydrogeology and water quality of the buried valleys has confirmed the nitrate problem and led to a recognition that some well waters with low (〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:0017467X:GWAT755:les" location="les.gif"/〉 5 mg/l) NO−3 concentrations have anomalous (〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:0017467X:GWAT755:ges" location="ges.gif"/〉 0.5 mg/l) ammonium ion (NH+4) levels, with an NH+4 range from 〈0.1 to 4.8 mg/l. The extractable NH+4 concentrations in related glacial sediments range up to approximately 75 mg/kg, and the amounts generally increase from an average of 2 mg/kg in the topsoil downward to bedrock. Migration of brines from subjacent Permian or Pennsylvanian bedrock into the unconsolidated sediments locally may cause desorption of NH+4 and an increase in its levels in the associated ground waters. Numerous test holes drilled in the study area showed a black scum on the fluid and cuttings, which may be from buried humic materials. Recently measured total organic carbon (TOC) contents of the ground waters confirm a significant level in some areas, with a range from 0.1 to 2.4 mg/l as C. Chlorination of water with dissolved organics may lead to production of halogenated compounds. Two public-water-supply well waters contained total trihalomethane (TTHM) levels close to the maximum contaminant level (MCL) of 100 /μg/l in chlorinated samples quenched after one week. The presence of NH+4 inhibits the formation of THMs, but it also can give rise to odor and taste problems in the finished water. The inhibition of THM formation by NH+4 is achieved by reactions which compete with the organics for combination with chlorine. These reactions make maintaining appropriate chlorine residuals difficult and also may lead to production of undesirable side products. Present efforts to evaluate the regional water-quality problems are focused in Nemaha County, Kansas.

Notes: Biofilm processes are potentially important for transformations of organic micropollutants in ground water. Some theoretical hypotheses and empirical observations suggest that a concentration threshold exists for some compounds below which the concentration cannot be reduced by bacterial action. However, in the presence of one compound at a relatively high concentration, termed the primary substrate, another compound present at trace concentrations, termed the secondary substrate, can be biotransformed as well. These concepts were evaluated through laboratory column studies with several halogenated organic compounds of importance in ground water. A biofilm model can successfully describe utilization of trace substrates, and application to modeling the subsurface is discussed. A simplified batch model with first-order kinetics may be adequate for describing subsurface microbial processes when low active organism and pollutant concentrations exist over a large scale.

Notes: Mathematical modeling founded on a strong field data base can be a valuable tool for the analysis of ground-water contamination problems. The purposes of this paper are threefold: (1) we demonstrate the dilemma of a knowledgeable ground-water quality regulator whose regulatory decision-making process is confronted with the output of a mathematical model that is based on very limited field test data; (2) we demonstrate a method available to a knowledgeable regulator for assessing approximately a range of possible performances of a contaminated ground-water recovery well field using a range of input data derived from a very limited data base; and (3) we present a strong case for presenting mathematical model outputs as ranges of values rather than as unique solutions. A range is determined by an examination of the level of sophistication of the field data base. Our experience with 12 field sites wherein ground-water contamination has occurred has led us to conclude that field data are seldom, if ever, adequate to defend a unique solution from a mathematical model. Regulatory decisions generally can be reduced to minimization of risks based on the smallest range of model outputs that can be defended on the basis of the field data base. The more limited the field data base, the greater must be the range of defensible model outputs, and consequently, the greater the risk inherent in subsequent regulatory decisions. The knowledgeable regulator can assess the risks in the regulatory decision-making process only if he is able to assess the extent to which the field data base for the mathematical model output reflects state-of-the-art data collection and analysis technologies and methodologies. If an applicant for a permit or license submits a less than adequate data base and concomitantly a broad range of defensible model outputs, he inherently requests that the knowledgeable regulator accept a risk greater than that required if adequate aquifer testing techniques had been employed.

Notes: Ground-water monitoring to delineate a contaminant plume in fluvial hydrostratigraphic units often is uncertain. Fluvial deposits consist typically of interbedded layers of sands, silts and clays, with buried stream channel deposits of sands or gravels. The channel deposits are often interpreted erroneously to be discontinuous between test holes and in cross section due to their sinuosity. Erroneous conclusions pertaining to the areal continuity of these geometrically complex deposits are inevitable unless the investigator thoroughly understands the depositional environment(s).The hydraulic conductivity of buried stream channel deposits may be several orders of magnitude higher than the matrix materials in which they are enclosed. The higher hydraulic conductivity of buried stream channel deposits has potentially significant ramifications with respect to ground-water monitoring to delineate the geometry of a contaminant plume migrating through these deposits.Ground-water monitoring at uranium mill waste disposal sites located in fluvial environments began on a significant scale in about 1977. A uranium mill tailing disposal site located in such an environment in central Wyoming is among the first sites monitored. Thirty-seven monitor wells were constructed at the site to delineate a seepage plume originating from one of the tailing ponds. This case history illustrates the need for a detailed under—standing of the hydrostratigraphy at a waste disposal site in order to interpret the meaning of ground-water quality data effectively. Water quality data from monitor wells located on a hit or miss basis often are misleading. The hydrostratigraphic horizon from which a water quality sample is collected must be well defined before the sample analyses can be interpreted quantitatively.

Notes: Deep-well injection into fractured sandstone is an option for the disposal of contaminated mine dewatering discharge from an open pit uranium mine. As part of the assessment of potential contaminant migration from deep-well injection, the effect of matrix diffusion was evaluated. An analytical mathematical model was developed for the simulation of the radial movement of a contaminant front away from an injection point under steady flow conditions in a planar fracture with uniform properties. The model includes the effects of advection in the fracture, diffusion of contaminants from the fracture into the rock matrix, and equilibrium adsorption on the fracture surface as well as in the rock matrix. Effective diffusion coefficients obtained from laboratory experiments on 11 intact core samples varied from 3.4 × 10−8 to 3.2 × 10−7 cm2/s. Model simulations were made with diffusion coefficient values in this range and with single-fracture injection rates estimated from fracture frequencies in boreholes, and from bulk hydraulic conductivity values obtained from field tests. Because of matrix diffusion, the rate of outward movement of the front of the nonreactive contaminants from the injection well is much slower than the rate of water flow in the fractures. Simulations of the movement of contaminants that undergo adsorption indicate that even a small distribution coefficient for the rock matrix causes the contaminants to remain very close to the injection well during the one-year period. The results of the simplified model demonstrate that matrix diffusion is an important process that cannot be neglected in the assessment of a waste disposal scheme located in fractured porous rock. However, in order to make a definitive assessment of the capability of matrix diffusion and associated matrix adsorption to significantly limit the extent of contaminant migration around injection wells, it would be necessary to conduct field tests such as a preliminary or experimental injection.

Notes: An abandoned creosote facility in Conroe, Texas, has become a field site for the National Center for Ground Water Research (NCGWR) at Rice University. Ground-water contamination in the shallow aquifer beneath the site was characterized by sampling soils and water quality at 14 monitoring wells and 35 boreholes.Results from six sampling trips over two years for inorganic and organic chemical concentrations in the ground water show wells around the site were contaminated to levels above 800 μg/l for naphthalene, 400 μg/1 for methyl naphthalene, and 150 μg/1 for dibenzofuran. Conservative constituents, traced by chloride concentrations up to 75 mg/l, have migrated 300 ft (90 m) downgradient of the site. Organic contaminants have been adsorbed and microbially degraded in their migration from the waste source as evidenced by their attenuated concentrations. Detailed field pump tests have been performed to evaluate hydraulic conductivity at several of the shallow wells. The U.S. Geological Survey (USGS) Solute Transport Model (Konikow and Bredehoeft, 1978) has been used to predict chloride plume patterns and evaluate parameters which govern transport processes at the Conroe waste site.

Notes: Treatment of vacuum-packed poultry pieces and muscle fillets with potassium sorbate decreased the rate of microbial development and increased shelf life when the substrates were stored at chill temperatures. Microbial growth on sorbate treated breast fillets was totally inhibited for up to 35 days at 2°C. The effect of sorbate was temperature dependent and completely negated by severe temperature abuse (storage at 12°C). Increased storage temperature also resulted in a greater proportion of gram-negative, facultatively anaerobic bacteria in the spoilage association. At each storage temperature where significant growth occurred, the effect of sorbate was to reduce the proportion of enteric organisms and increase the proportion of lactic acid bacteria in the spoilage association.

Notes: Freezing and thawing of Campylobacter jejuni inflicted sublethal injury among survivors. About 95% or more of the survivors lost viability within 2 h of microaerobic incubation at 42°C in brucella broth containing a Campylobacter-selective antibiotic supplement of vancomycin, trimethoprim, polymyxin B, cephalothin and amphotericin. The remaining viable cells were able to multiply under these incubation conditions. The stressed cells were adversely affected by incubation at 42°C andpolymyxin B. A method consisting of incubating suspected samples microaerobically in brucella broth, supplemented with nutrients (succinate + cysteine) and antibiotics (vancomycin, trimethoprim, cephalothin and amphotericin) at 37°C for the initial 6 h and then adding polymyxin B and shifting the temperature to 42°C up to 24 h could improve detection of C. jejuni from frozen foods.

Notes: By using a method which permitted the selection of repaired cells from a population of heat-injured and noninjured cells of Staphylococcus aureus 196E, we were able to determine that the progeny of repaired cells retained the ability to produce enterotoxin A (SEA). There were large variations in the amount of SEA produced by the progeny of individual colony forming units (CFU) before and after heating. The average amount of SEA produced by the progeny of noninjured and repaired staphylococci were similar and not significantly different.

Notes: Of 21 food flavoring compounds tested against 39–40 organisms, 10 were ineffective at 1,000 ppm or 10 mM either at pH 6 or 8 and by either surface plating or in broth. The 11 effective compounds were d- and l-carvone, diacetyl, ethyl vanillin, eugenol, maltol, menthol, phenylacetic acid, phenylacetal-dehyde, 2,3-pentanedione and vanillin; with diacetyl and eugenol being the most effective. All were more effective at pH 6 than at pH 8. With the exception of maltol and phenylacetic acid, each was generally more effective against fungi than bacteria with the lactic acid bacteria being the most resistant. At pH 5.5 and 5°C, diacetyl was inhibitory to Pseudomonas fluorescens and P. geniculata at 〈 10 ppm. The findings suggest that compounds used in acid foods, ostensibly as flavoring agents, may exert antimicrobial effects when considered in context with all parameters of growth.

Notes: Water absorption and changes in surface microtopography are major determinants of contamination of the underside of neck tissue. Salmonella cells adhere firmly to this tissue but cannot be effectively removed because of the major contribution of physical entrapment to the overall level of contamination. Heat and/or sorbate treatments can bring the bacteriological quality of neck tissue to that of the rest of the carcass but removal of this tissue is the most effective means of decontamination.

Notes: A study to assess possible exposure to carcinogenic metabolites (aflatoxins) from a mold Aspergillus flavus has been conducted in a rice producing area of Brazoria County, Texas. One hundred samples of unmilled rice were analyzed by thin-layer chromatography (TLC) for the amount of aflatoxin produced by the mold during rice growth and storage. Two well water samples and two rice elevator dust samples were also checked for possible aflatoxin content. The cancer mortality rates (gastrointestinal and urinary tracts cancers) in the rice-growing and nonrice-growing areas of the same county were compared.No aflatoxin was detected by TLC methods in rice, rice dusts or water samples. When extracts of rice dusts were checked for mutagenesis by the Ames Salmonella assay as a supplement to the TLC analysis, the results suggested that these dusts might have contained mutagenic material. This observation notwithstanding, we found no evidence that the rice produced in the studied part of the Gulf Coast had a problem of aflatoxin contamination. Also, cancer mortality rates for two major organ systems were not found to differ for rice-producing and nonrice-producing areas of rural Brazoria County.

Notes: This review supplements the review by Hargreaves et al. (1972). Phosphate selection in the U.S. continues to be based upon achieving specific functional objectives other than microbial control. Current federal regulations limit the addition of phosphates to those levels which will achieve functionality. One notable exception is shelf stable pasteurized process cheese, cheese food, and cheese spreads. Adding relatively high levels of phosphates for emulsification coincidentally provides microbiological stability; however, the minimum levels for stability remain uncertain.It is becoming increasingly evident that phosphates, under certain conditions, have potential value for enhancing the microbial safety and stability of foods. Certain phosphates or mixtures of phosphates are clearly more effective than others. Through future research, it should be possible to further exploit the potential value of phosphates. This review offers direction for such research.