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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 148 (1999), S. 37-40 
    ISSN: 1573-0832
    Keywords: Fumonisin B1 ; maize ; poultry feeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A total of 100 maize and 50 poultry feed samples collected in 1998 at random from nine and eight districts of Haryana, respectively, were analysed for fumonisin B1. The samples were collected from poultry farms, feed manufacturers and markets. Ninety one (91%) maize samples and forty two (84%) poultry feed samples were found to contain fumonisin B1. Fumonisin B1 contamination in the maize samples ranged from 0.1–87.0 ppm. Whereas the poultry feed samples contained fumonisin B1 in the range of 0.02–28.0 ppm. It indicated widespread prevalence of fumonisin B1 in maize and poultry feeds in different areas of Haryana.
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  • 2
    Electronic Resource
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    Springer
    Nutrient cycling in agroecosystems 55 (1999), S. 175-185 
    ISSN: 1573-0867
    Keywords: fertilizer recommendation ; maize ; soil mineral nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fifteen field trials were conducted to evaluate soil mineral N measurement as a means for quantifying the total N supply to forage maize and so to form the basis for fertilizer recommendations on a crop-specific basis. In every trial, 4 rates of cattle manure N (nominally 0, 80, 160, 240 kg N per ha) and 4 rates of ammonium nitrate (0, 50, 100, 150 kg N per ha) were factorially combined. Soil mineral N measurements were made before manure application, at the time of maize drilling, 7-10 weeks after drilling and after harvest. Measurements on control treatments which received no manure or ammonium nitrate showed extensive net mineralisation of soil N (mean 140 kg N per ha) in the 7-10 weeks after drilling followed by a decrease due to crop uptake, and probably net immobilisation, of approximately the same amount by harvest. This net mineralisation was probably the reason why only one trial showed a significant dry-matter yield response to ammonium nitrate. Results indicated that , to be useful for N recommendations, soil mineral N measurements should be taken 7-10 weeks after drilling. Only if the amount of mineral N at this time is less than expected crop N offtake should fertilizer N be applied. A mean of around 64% of the N applied in ammonium nitrate could be accounted for in soil mineral N after harvest of the maize, although this was reduced to 24% in the single trial where a dry-matter response to ammonium nitrate was recorded.
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  • 3
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    Nutrient cycling in agroecosystems 55 (1999), S. 95-105 
    ISSN: 1573-0867
    Keywords: cover crops ; savanna ; N fertilizer ; maize ; cowpea ; N fertilizer replacement value
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Integrated soil management with leguminous cover crops was studied at two sites in the northern Guinea savanna zone of northern Nigeria, Kaduna (190 day growing season) and Bauchi (150 days). One-year planted fallows of mucuna, lablab, and crotalaria were compared with natural grass fallow and cowpea controls. All treatments were followed by a maize test crop in the second year with 0, 30, or 60 kg N ha−1 as urea. Above ground legume residues were not incorporated into the soil and most residues were burned early in the dry season at the Kaduna site. Legume rotation increased soil total N, maize growth in greenhouse pots, and dry matter and N accumulation of maize. Response of maize grain yield to 30 kg N ha−1 as urea was highly significant at both sites and much greater than the response to legume rotation. The mean N fertilizer replacement value from legume rotation was 14 kg N ha−1 at Kaduna and 6 kg N ha−1 at Bauchi. W ith no N applied to the maize test crop, maize grain yield following legume fallow was 365 kg ha−1 higher than natural fallow at Bauchi and 235 kg ha−1 higher at Kaduna. The benefit of specific legume fallows to subsequent maize was mostly related to above ground N of the previous legume at Bauchi, where residues were protected from fire and grazing. At Kaduna, where fallow vegetation was burned, maize yield was related to estimated below ground N. The results show that legume rotation alone results in small maize yield increases in the dry savanna zone.
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  • 4
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    Journal of the history of biology 32 (1999), S. 133-162 
    ISSN: 1573-0387
    Keywords: McClintock ; Barbara ; maize ; corn ; genetics ; transposable elements ; controlling elements ; gene expression regulation ; women scientists ; development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , History
    Notes: Abstract In the standard narrative of her life, Barbara McClintock discovered genetic transposition in the 1940s but no one believed her. She was ignored until molecular biologists of the 1970s “rediscovered” transposition and vindicated her heretical discovery. New archival documents, as well as interviews and close reading of published papers, belie this narrative. Transposition was accepted immediately by both maize and bacterial geneticists. Maize geneticists confirmed it repeatedly in the early 1950s and by the late 1950s it was considered a classic discovery. But for McClintock, movable elements were part of an elaborate system of genetic control that she hypothesized to explain development and differentiation. This theory was highly speculative and was not widely accepted, even by those who had discovered transposition independently. When Jacob and Monod presented their alternative model for gene regulation, the operon, her controller argument was discarded as incorrect. Transposition, however, was soon discovered in microorganisms and by the late 1970s was recognized as a phenomenon of biomedical importance. For McClintock, the award of the 1983 Nobel Prize to her for the discovery of movable genetic elements, long treated as a legitimation, may well have been bittersweet. This new look at McClintock's experiments and theory has implications for the intellectual history of biology, the social history of American genetics, and McClintock's role in the historiography of women in science.
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  • 5
    ISSN: 1573-4919
    Keywords: lipid ; transfer ; binding ; proteins ; plants ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Plant cells contain lipid-transfer proteins (LTPs) able to transfer phospholipids between membranes in vitro. Plant LTPs share in common structural and functional features. Recent structural studies carried out by NMR and X-ray crystallography on an LTP isolated from maize seeds have showed that this protein involves four helices packed against a C-terminal region and stabilized by four disulfide bridges. A most striking feature of this structure is the existence of an internal hydrophobic cavity running through the whole molecule and able to accomodate acyl chains. It was thus of interest to study the ability of maize LTP to bind hydrophobic ligands such as acyl chains or lysophosphatidylcholine and to determine the effect of this binding on phospholipid transfer. The binding abilities of maize LTP, presented in this paper, are discussed and compared to those of lipid-binding proteins from animal tissues.
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  • 6
    ISSN: 1573-8469
    Keywords: ear rot ; leaf blight ; mid-altitude ; maize ; Stenocarpella macrospora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inheritance of resistance to Stenocarpella macrospora (Earle) Sutton (syn. Diplodia macrospora Earle) ear rot of maize was studied among selected maize populations in the mid-altitude (1280 m) agro-ecological zone of Nigeria. Diallel analysis among the populations showed significant values for general combining ability (GCA) and specific combining ability (SCA) effects at 5% and 1% levels respectively. Variance components of GCA and SCA on Stenocarpella ear rot were 0.019 and 0.627 respectively, indicating that non-additive genes play major roles in the inheritance of Stenocarpella ear rot resistance. The GCA and SCA effects were relatively dependent on the materials involved in the evaluations. Generation mean analysis was used on five selected parent inbreds (2 resistance and 3 susceptible crossed to give P1, P2, F1, BC1, BC2 and F2 generations). Estimates of the six parameters on ear rot indicate that dominance gene effects made the major contribution to variation in ear rot of maize in the crosses studied. The magnitude and significance of the estimates for digenic effects in the crosses suggest that epistatic gene effects are present and important in the basic mechanism of Stenocarpella ear rot inheritance in the populations studied. Additive effects have only minor importance in the total variation.
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  • 7
    ISSN: 1573-5028
    Keywords: maize ; protoderm ; epidermis ; homeobox ; HDGL2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The formation of a morphologically distinct outer cell layer or protoderm is one of the first and probably one of the most important steps in patterning of the plant embryo. Here we report the isolation of ZmOCL1 (OCL for outer cell layer), a member of the HDGL2 (also known as HD-ZIP IV) subclass of plant-specific HD-ZIP homeodomain proteins from maize. ZmOCL1 transcripts are detected very early in embryo development, before a morphologically distinct protoderm is visible, and expression then becomes localised to the protoderm of the embryo as it develops. Subsequently, expression is observed in the L1 cell layer of both the developing primary root and shoot meristems, and is maintained in developing leaves and floral organs. We propose that ZMOCL1 may play a role in the specification of protoderm identity within the embryo, the organisation of the primary root primordium or in the maintenance of the L1 cell layer in the shoot apical meristem. We also show that the expression of ZmOCL1 is different from that of another epidermal marker gene, LTP2 (lipid transfer protein) and, in meristems, is complementary to that of Kn1 (Knotted) which is transcribed only in underlying cell layers.
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  • 8
    ISSN: 1573-5028
    Keywords: lignification ; maize ; proline-rich protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A clone encoding a proline-rich protein (ZmPRP) has been obtained from maize root by differential screening of a maturing elongation root cDNA library. The amino acid sequence deduced from the full-length cDNA contains a putative signal peptide and a highly repetitive sequence containing the PEPK motif, indicating that the ZmPRP mRNA may code for a cell wall protein. The PEPK repeat is also found in a previously reported wheat sequence but differs from the repeated sequences found in hydroxyproline-rich glycoproteins (HRGP) and in dicot proline-rich proteins (PRP). In the maize genome, the ZmPRP protein is encoded by a single gene that is expressed in maturing regions of the root, in the hypocotyl and in the pericarp. In these organs, the ZmPRP mRNA accumulates in the xylem and surrounding cells, and in the epidermis. No ZmPRP mRNA was found in the phloem. The pattern of mRNA accumulation is very similar to the one observed for genes coding for proteins involved in lignin biosynthesis and, like most cell wall proteins, ZmPRP synthesis is also induced by wounding. These data support the hypothesis that ZmPRP is a member of a new class of fibrous proteins involved in the secondary cell wall formation in monocot species.
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  • 9
    ISSN: 1573-5028
    Keywords: ABRE ; embryogenesis ; G-box ; gene expression ; maize ; protein-DNA interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcription of the rab28 gene from maize is induced in late embryo development and in response to abscisic acid. We have studied the regulation of the activity of the rab28 promoter in embryos. Two abscisic acid-responsive elements (ABREs) were necessary for expression in embryos of transgenic Arabidopsis and in transient transformation in maize embryos. In vivo footprinting showed that there was protein binding to the ABREs and to other cis elements in the promoter in young embryos before expression of rab28. This shows that the rab28 promoter is in an open chromatin structure before developmental activation. The ABREs are important for the induction and have protein binding in young embryos. Nuclear proteins extracted from embryos before activation of rab28 bound to the ABREs in band shift assays. A complex with different mobility was formed between nuclear proteins and the ABREs after induction of rab28 suggesting a modification of the ABRE-binding factor or an exchange of proteins. The footprints on the ABREs were unaltered by induction with abscisic acid or during developmental activation of rab28. These results indicate that constitutive binding of transcription factor(s) on the ABRE is central in embryonic regulation of the rab28 gene.
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  • 10
    ISSN: 1573-5028
    Keywords: Coix lacryma jobi ; dihydrodipicolinate synthase ; GCN4 ; lysine ; maize ; opaque2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dihydrodipicolinate synthase (DHPS) is the main enzyme of a specific branch of the aspartate pathway leading to lysine biosynthesis in higher plants. We have cloned and characterized the DHPS-encoding DapA gene from the maize-related grass Coix lacryma-jobi. The DapA open reading frame is interrupted by two introns and encodes the 326 amino acid-long Coix DHPS protein, which is 95% identical to the maize DHPS protein. Coix DNA gel blot analysis with maize DHPS cDNA as a probe showed a single strongly hybridizing band along with faint bands. RNA gel blot analysis showed that DHPS transcripts are present in coleoptiles, embryos, endosperms, and roots but are almost undetectable in blades of young leaves of both Coix and maize. The 5′-flanking region of the DapA gene contains a TGACTC GCN4-like element located 372 bp upstream the putative translation start codon. Steady-state levels of DHPS mRNA were slightly reduced in the endosperms and embryos of the maize lysine-rich opaque2 mutants when compared with those in normal kernels. Selective binding assay with the maize Opaque2 protein (O2) showed that the GCN4-like element is not an O2 binding site, suggesting that the DHPS gene is not under the control of O2.
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  • 11
    ISSN: 1573-5028
    Keywords: BETL ; endosperm ; maize ; promoter ; transfer cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In maize, a layer of basal endosperm cells adjacent to the pedicel is modified for a function in solute transfer. Three genes specifically expressed in this region, termed the basal endosperm transfer layer (BETL-2 to -4), were isolated by differential hybridization. BETL-2 to -4 are coordinately expressed in early and mid-term endosperm development, but are absent at later stages. BETL-2 to -4 coding sequences all predict small (〈100 amino acids), secreted, cysteine-rich polypeptides which lack close relatives in current database accessions. BETL-3 and BETL-1 display some sequence similarities with each other and to plant defensins. BETL-2 to -4 promoter regions were isolated and compared, revealing the presence of a promoter-proximal microsatellite repeat as the most highly conserved sequence element in each sequence. Electrophoretic mobility shift assays (EMSA) showed that specific BETL-2 to -4 promoter fragments competed for binding to the same DNA-binding activity in nuclear extracts prepared from maize endosperm. Although BETL-2 to -4 are only expressed in basal endosperm cells, the DNA-binding activities detected were of two types: distal endosperm-specific, or present in both basal and distal endosperm extracts. On the basis of these findings, a model to account for the coordinate regulation of BETL genes in endosperm cells is proposed.
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  • 12
    ISSN: 1573-5036
    Keywords: maize ; matric water potential ; rhizosphere ; soil:root adhesion ; soil:root contact ; soil surface properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was designed to investigate the strength of attachment of plant seedling roots to the soil in which they were grown. The study also assessed the effects of differing soil textures and differing soil matric potentials upon the strength of the root:soil attachment. A device for growing roots upon a soil surface was designed, and was used to produce roots which were attached to the soil. In order to quantify root:soil adhesion, roots of maize seedlings, grown on the soil surface, were subsequently peeled off using a universal test machine, in conjunction with simultaneous time-lapse video observation. To clarify the partitioning of energy in the root:soil peeling test, separate mechanical tests on roots, and on two adherent remoulded topsoil balls were also carried out. The seedling root was characterised by a low bending stiffness. The energy stored in bending was negligible, compared to the root:soil adhesion energy. The mechanical properties of two adherent remoulded topsoil balls were a decrease of the soil:soil adhesion energy as the soil:soil plastic energy increased. These two parameters were therefore interdependent. Using a video-camera system, it was possible to separate the different processes occurring during the root:soil peeling test, in particular, the seed:soil adhesion and the root:soil soil adhesion. An interpretation of the complex and variable force:displacement curves was thus possible, enabling calculation of the root:soil interfacial rupture energy. At a given suction (10 kPa), the results of the peeling test showed a clear soil texture effect on the value of the root:soil interfacial rupture energy. In contrast, for the same silty topsoil, the effect of the soil water suction on the value of the interfacial rupture energy was very moderate. The root:soil interfacial rupture energy was controlled mainly by a product of microscopic soil specific surface area and the macroscopic contact surface area between the root and the soil. Biological and physical interactions contributing to root:soil adhesion such as root:soil interlocking mechanics were also analysed and discussed.
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  • 13
    ISSN: 1573-5036
    Keywords: calcite ; goethite ; maize ; phosphate ; H+ release ; Brassica napus L. ; Zea Mays L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In calcareous soils the dynamics of phosphorus is controlled by calcite and iron oxides such as goethite which strongly retain P and consequently maintain low P concentrations in soil solution. Plants can drastically change chemical conditions in the rhizosphere, in particular by releasing H+ or OH− or by excreting organic anions. By modifying the dissolution/precipitation and desorption/adsorption equilibria, roots can influence the mobility of soil P. The aim of this work was to test whether H+ or OH− release can induce the mobilization of P in the rhizosphere of maize and rape supplied with NO3-N or NH4-N and grown on synthetic phosphated calcite or goethite as sole source of P. With P-calcite, the mobilization of P was generally related to the acidification of the rhizosphere. With P-goethite, rhizosphere acidification induced some increase of DTPA-extractable Fe and hence dissolution of goethite. Rhizosphere P was concomitantly depleted but the mechanisms involved are less clear. The difference in behavior of the two species is discussed.
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  • 14
    ISSN: 1573-5036
    Keywords: agroforestry ; Gliricidia sepium ; inorganic nitrogen ; maize ; nitrogen mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Many soils of southern Africa are severely N deficient, but inorganic fertilizers are unaffordable for most subsistence farmers. Rotations and intercrops of legumes with crops may alleviate N deficiency through biological N2 fixation and redistribution of subsoil N to the surface. We monitored soil inorganic N dynamics for two seasons in a gliricidia [Gliricidia sepium (Jacq.) Walp.] – maize (Zea mays L.) intercrop in the unimodal rainfall area of southern Malawi. One maize crop per year was grown with or without interplanted gliricidia, in factorial combination with three rates of N (0, 24 or 48 kg N ha-1). Application of gliricidia prunings increased (p 〈 0.001) topsoil (0 to 20 cm) inorganic N at the end of the dry season and during the early rains. Differences between plus and minus gliricidia treatments were less when total inorganic N to 1-m depth was summed. A greater proportion of the total inorganic N to 1-m depth occurred in the topsoil (0 to 20 cm) when gliricidia was present, suggesting that redistribution of subsoil N to the surface accounted for part of the N increase by gliricidia. Gliricidia lowered (p 〈 0.05) subsoil water content during drier periods. Gliricidia plots accumulated more (p 〈 0.01) ammonium-N during the dry season. Nitrate-N remained constant during the dry season but rose rapidly in gliricidia plots after the onset of rains. A 2-factor model including preseason inorganic N and anaerobic N mineralization potential accounted for 84% of the variability in maize yields for the two seasons' data combined. The combination of preseason inorganic N and potential N mineralization appears to provide a good estimate of N supply to maize in systems receiving both organic and inorganic sources of N.
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  • 15
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    Euphytica 108 (1999), S. 145-150 
    ISSN: 1573-5060
    Keywords: maize ; North Carolina model 2 ; salinity tolerance ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic basis of salt tolerance was examined in selected salt tolerant and sensitive material from a sample of accessions previously assessed for variability in salinity tolerance. The North Carolina Model 2 Design and analysis was followed, tolerance being assessed in 10-day-old seedlings grown in salinized solution culture at control (0 mM), 60 mM and 80 mM NaCl concentrations). Salinity tolerance was shown to be under the control of genes with additive and non-additive effects, with broad and narrow sense heritability estimates being approximately 0.7 and 0.4 over all treatments.
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  • 16
    ISSN: 1573-5079
    Keywords: chlorophyll a biosynthesis ; etiolated leaves ; heat shock ; maize ; pea ; pea mutants ; Photosystem II core ; protochlorophyllide photoreduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Preliminary dark incubation of etiolated pea and maize plants at 38 °C allowed to observe a new dark reaction of Chl biosynthesis occuring after photoconversion of protochlorophyllide Pchld 655/650 into chlorophyllide Chld 684/676. This reaction was accompanied by chlorophyllide esterification and by the bathochromic shift of pigment spectra: Chld 684/676 → Chl 688/680. After completion of the reaction, a rapid (20–30 s at 26 °C) quenching of Chl 688/680 low-temperature fluorescence was observed. The reaction Chld 684/676 → Chl 688/680 was inhibited under anaerobic conditions as well as in the presence of KCN; the reaction accompanied by Chl fluorescence quenching was inhibited in the leaves of pea mutants with impaired function of Photosystem II reaction centers. The spectra position of newly formed Chl, effects of Chl fluorescence quenching allowed to assume that the new dark reaction is responsible for biosynthesis of P–680, the key pigment of Photosystem II reaction centres.
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  • 17
    ISSN: 1573-6857
    Keywords: Ac transposase binding site ; functional gene categories ; genomics ; hexamer ; maize ; motif signatures ; nonrandom distributions ; pentamer ; repeat motifs ; Tourist motif ; transposable elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several transposable elements (TEs) have been reported in association with genes in maize and other plants. In this study we found, based on statistical analyses of 951 DNA sequences within a maize computer database, that short hexamer and pentamer DNA motifs from the Activator (Ac) and from the Tourist TEs, respectively, were also associated with maize genes. Moreover, these two short hexamer and pentamer TE motifs were nonrandomly and nearly nonrandomly distributed, respectively, with respect to particular biochemical functions of those maize genes. To determine whether this distribution may be unique to TE motifs, or may be more widespread among hexamers/pentamers in general, we similarly studied six additional hexamer or pentamer sequences not derived from TEs. These also showed nonrandom distribution with respect to functional gene categories in the maize database. However, each of the total of eight short sequence motifs we studied differed in its pattern of association with distinct sets of functional gene categories; that is, there was a unique ‘signature’ for each of the hexamers and pentameters tested. Potential biological hypotheses to explain these findings are discussed.
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  • 18
    ISSN: 1573-1561
    Keywords: European corn borer ; Ostrinia nubilalis (Hübner) ; growth ; development ; fecundity ; oviposition ; behavior ; eggs ; DIMBOA ; water extract ; plant extract ; maize ; Peruvian maize ; maize accessions ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Twelve Peruvian maize, Zea mays, accessions were selected because of their relatively high level of field resistance to first-generation European corn borer (ECB), Ostrinia nubilalis, larval leaf-feeding. Water extracts of freeze-dried, powdered, leaf tissue were incorporated into a standard ECB diet, fed to larvae, and the effects on larval growth, development, and fecundity were measured. Larval and pupal weights were monitored as were the time elapsed in the larval, pupal, and adult stages. Adult fecundity and egg fertility were recorded. The experiment was a randomized block design (larvae and pupae) or a completely randomized design (adults) and analyzed with ANOVA (α = 0.05). Pairwise comparisons were made between groups of insects grown on diets containing extracts from the Peruvian lines, a standard diet, or diets containing extracts of a known susceptible inbred, and a known resistant inbred line. Survival was analyzed with a chi-squared test (α = 0.05). Two Peruvian accessions significantly reduced female larval and pupal weights, extended pupal and adult development time, and decreased survival of pupae and adults. Water extracts also had a pronounced impact on males; two accessions significantly reduced pupal weight and extended the time required to pupate, and one reduced male survival to adults. The results indicate that water-soluble factors from resistant Peruvian accessions inhibit the growth, developmental time, and survival of ECB. These resistance factors could be useful in the development of maize germplasm with insect-resistant traits.
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  • 19
    ISSN: 1573-0867
    Keywords: applied N and P ; crop residue ; maize ; Residual N and P ; Typic Pellustert ; Typic Ustorthent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field trials were conducted on two soil types for seven years (1988–1994) to investigate grain yield response of maize to crop residue application as influenced by varying rates of applied and residual N and P fertilizers. Yearly application of N and P fertilizers at both one-half and full recommended rates resulted in grain yield increases of more than 500 and 1100 kg ha-1, respectively over application of only crop residue. Moreover, grain yield responses due to residual N and P fertilizers applied only during the first year were found to be comparable to the yearly applications of these fertilizers. Rainfall and soil type have exerted considerable influences on the grain yield response obtained in this study. Grain yield exhibited a corresponding decrease with decreasing rainfall. Grain yield increases on Typic Pellustert were relatively higher than on Typic Ustorthent.
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  • 20
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    Agroforestry systems 47 (1999), S. 67-91 
    ISSN: 1572-9680
    Keywords: cover crops ; farmer innovation ; land tenure ; maize ; nutrient cycling ; velvetbean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In many parts of Central America long fallow periods are no longer feasible due to increasing land pressures. Farmers in northern Honduras have developed and diffused from farmer-to-farmer a maize cropping system using velvetbean (Mucuna spp.) as a short-term fallow. High total annual rainfall in a bimodal distribution is favorable to the system because it allows the completion of two rainfed cropping cycles annually. The first season is dedicated to the production of the mucuna crop and the second season is dedicated to maize. Soil pH and exchangeable Ca were not reduced during a 15-year period of continuous mucuna use. Soil organic matter, infiltration, and porosity increased with continuous mucuna use. Maize yields in fields with continuous rotation of mucuna were on average double those obtained without mucuna. The mucuna system was more profitable than the existing alternative bush-fallow system due to higher returns to land and labor resulting from higher yields, lower weeding and land preparation costs, and reduced risk of drought stress. The relative profitability of the mucuna system was also enhanced by seasonally high maize prices during the second season when maize is harvested in the mucuna system. Relatively easy access to land through inexpensive land ownership and land rental markets has made it possible for even small-scale farmers to dedicate land to the mucuna system. Land-use intensity is increasing in the region, however, as land is converted to pastures for cattle production. The opportunity costs of keeping land in the mucuna system, while also accessing land for first-season maize and other crops, are also increasing. These experiences remind us that a viable livelihood is the primary factor in farmers' decision making about adoption particular components of farming systems.
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  • 21
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    Plant molecular biology 39 (1999), S. 1063-1071 
    ISSN: 1573-5028
    Keywords: cell cycle ; gene expression ; in vitro fertilization ; maize ; zygote ; embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differential screening of cDNA libraries of unfertilized egg cells and in vitro zygotes of maize resulted in the isolation of more than 50 different genes whose expression is up- or down-regulated after in vitro fertilization (IVF). Amoung these genes, we identified a cDNA encoding the eukaryotic translation initiation factor eIF-5A. This highly conserved factor is thought to be necessary for selective mRNA stabilization and translation. It is also the only known protein that contains the unusual amino acid hypusine which is required for biological activity. High transcript amounts are stored in the egg cell, which is, in terms of metabolism, relatively inactive. Upon fertilization transcript amounts decrease, in contrast to metabolically inactive embryos in which the transcript cannot be detected and transcript levels increase upon germination. The expression pattern during the first embryonic cell cycle is also different from that observed during the somatic cell cycle: egg cells in the G0 phase contain high transcript levels, while arrested suspension cells contain few transcripts. In the somatic cell cycle, eif-5A is strongly induced during the G1 phase and transcripts are continuously degraded during the S, G2 and M phases until new induction during the G1 phase of the next cycle. eif-5A, a member of a small gene family in maize, is expressed in most maize tissues investigated. Based on our results, we suggest that the unfertilized egg cell of maize, although relatively inactive regarding its metabolism, is prepared for selective mRNA translation that is quickly triggered after fertilization. We also suggest that the regulation of eif-5A in the first embryonic cell cycle is different from the somatic cell cycle.
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  • 22
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    Plant molecular biology 41 (1999), S. 733-739 
    ISSN: 1573-5028
    Keywords: bifunctional enzymes ; dihydrofolate reductase ; DNA synthesis ; endoreduplication ; maize ; thymidylate synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A bifunctional gene (ZmDHFR-TS) encoding dihydrofolate reductase (DHFR) and thymidylate synthase (TS) was cloned from a Zea mays cDNA library. Both of these enzymes are involved in nucleotide biosynthesis, specifically in the formation of thymidine monophosphate (TMP). Comparison of the deduced amino acid sequence with DHFR-TS sequences from three other plant sources revealed over 75% similarity and motifs typical of DHFR-TS proteins. Two copies of the gene were mapped to chromosomes 2 and 4. This represents the first DHFR-TS gene cloned from a monocotyledonous plant. Expression of ZmDHFR-TS was examined in developing kernels and various tissues of maize by RNA gel blot hybridization analysis in order to determine the relationship between expression of this gene and DNA synthesis. RNA transcripts for ZmDHFR-TS accumulated to high levels in developing maize kernels when endosperm cells were undergoing endoreduplication and cell division. Meristematic maize tissues had high levels of ZmDHFR-TS mRNA, but transcripts were barely detectable in RNA isolated from the root elongation zone and from mature leaf tissues.
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  • 23
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    Plant molecular biology 41 (1999), S. 801-814 
    ISSN: 1573-5028
    Keywords: eEF1A ; endosperm ; gene family ; maize ; opaque2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract eEF1A appears to be a multifunctional protein in eukaryotes, where it serves as a protein synthesis factor as well as a cytoskeletal protein. In maize endosperm, the eEF1A concentration is highly correlated with lysine content, and eEF1A synthesis is increased in opaque2 mutants compared to wild type. To investigate the basis for the increased synthesis of eEF1A in opaque2, we characterized the genes encoding this protein and measured their relative level of expression in endosperm and other tissues. Maize contains 10 to 15 eEF1A genes that are nearly identical in nucleotide and amino acid sequences. However, these genes can be distinguished based on their 3′ non-coding sequences, which are less conserved. By screening endosperm and seedling cDNA libraries, we show that most of the maize eEF1A genes are expressed, and the relative level of their transcripts varies in different tissues. At least five genes are transcribed in the endosperm, and two account for ca. 80% of the RNA transcripts. The expression of several genes is enhanced in opaque2 endosperm, although the significance of this is unclear.
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  • 24
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    Biologia plantarum 42 (1999), S. 289-296 
    ISSN: 1573-8264
    Keywords: Acaulospora tuberculata ; alkaline phosphatase ; Deschampsia flexuosa ; extraradical mycelium ; Glomus fistulosum ; Glomus mosseae ; maize ; NADH-diaphorase ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simulated acid rain (SAR) combined with higher concentration of aluminium (SAR+Al) influenced the ecophysiology of three arbuscular mycorrhizal fungi (AMF) in both the germination and symbiotic phases of their life cycle. Acaulospora tuberculata, an isolate from the soil with low pH, exhibited a higher tolerance to environmental stress as compared to Glomus mosseae and G. fistulosum. This higher tolerance may be related to the edaphic conditions of soil of the isolate origin. The histochemical staining of the alkaline phosphatase and NADH-diaphorase activities in the extraradical mycelium (ERM) of the AMF proved to be more sensitive indication of negative effects of the SAR or SAR+Al stress compared to commonly measured parameters of the AMF such as mycorrhizal colonisation or growth of the ERM.
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  • 25
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    Entomologia experimentalis et applicata 89 (1998), S. 119-124 
    ISSN: 1570-7458
    Keywords: maize ; Zea mays ; Helicoverpa zea ; antibiosis ; flavonoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The corn earworm (Helicoverpa zea Boddie) is an important pest of corn (Zea mays L.), and its larvae sometimes cause severe ear damage to hybrids grown in the southeastern United States. The antibiotic compound isoorientin is present in silks of some corn inbreds at a concentration that is harmful to corn earworm larvae. The inbred T218, which produces biologically active levels of this compound (〈2.0% dry weight), was evaluated in hybrid combination with two other non-isoorientin producing inbreds to determine the mode of isoorientin inheritance in corn silks. Silk masses from individual ears of each parent, the F1, F2, first backcrosses, F3 families and selfed BC1 families were evaluated in 1994 and 1995 for isoorientin concentration. Reversed-phase high performance liquid chromatography (HPLC) was used to make chemical determinations. Segregation ratios in the F2, first backcross to T218, F3 families and selfed BC1 families were tested. The tests were conclusive in the identification of a single recessive gene controlling high isoorientin concentration in the silks of inbred T218. Some evidence for modifiers exists, in that there was a statistically nonsignificant trend for more plants than expected to occur in the low isoorientin concentration classes. Development of inbreds with a high concentration of the simply inherited isoorientin in their silks will add to the arsenal of compounds available in corn silks to combat damage to corn by corn earworm larvae.
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  • 26
    ISSN: 1573-0832
    Keywords: deoxynivalenol ; enzyme immunoassay ; feed ; maize ; mycotoxins ; wheat
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    Topics: Biology , Medicine
    Notes: Abstract Samples of wheat (n = 25) and maize (n = 30) for animal consumption, collected in 1997 after harvest from western Romania, were analyzed by enzyme immunoassays for mycotoxin contamination. Toxins analyses included deoxynivalenol (DON), 3-acetylDON, 15- acetylDON, fusarenone X (FX), T-2 Toxin (T-2), diacetoxyscirpenol (DAS), zearalenone (ZEA), fumonisin B1 (FB1), aflatoxin B1 (AFB1), ochratoxin A (OA), and citrinin (CT). DON and acetylDONs were the major contaminants in wheat (100%) and maize (46%). Median values for DON, 3-acetylDON, and 15-acetylDON were 880 μg kg-1, 66 μg kg- 1, and 150 μg kg-1 in wheat, and 890 μg kg-1, 180 μg kg-1, and 620 μg kg- 1 in maize, respectively. Additionally, 3,15-diacetylDON was detected in some samples by HPLC-EIA analysis. All samples were negative for FX (〈150 μg kg-1). T-2 was found in wheat (n = 6) and maize (n = 1) at levels between 13 and 63 μg kg- 1. DAS (2.6 μg kg-1) was found in one maize sample. ZEA occurred in all wheat and in four maize samples, median values were 10 μg kg-1 and 250 μg kg-1, respectively. One maize sample contained FB1 (140 μg kg-1). All samples were AFB1-negative (〈4 μg kg-1). OA was found in one wheat sample (37 μg kg- 1), CT was found in one maize sample (580 μg kg- 1). This first reported natural occurrence of a range of mycotoxins in Romanian feeding stuff shows that DON and acetyl DONs may be present at levels which may affect animal production.
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  • 27
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    Agroforestry systems 40 (1998), S. 41-58 
    ISSN: 1572-9680
    Keywords: hedgerow ; maize ; mulch ; Pennisetum purpureum
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Napier grass strips are gaining some acceptance in Southeast Asia as a vegetative means of soil erosion control, due to the relative ease of establishment and management. In addition, the napier grass strips aid productivity of agroforestry systems by providing mulch or by being fed to livestock. In this paper, a bioeconomic comparison of different ways of utilising the napier grass cuttings is undertaken. The three uses examined for the cuttings were: as mulch in the alley areas, sold as fodder, and fed to animals with manure applied back to the system. The analysis linked the soils-oriented SCUAF model with economic and animal component models. SCUAF was parameterized using field data based on farmer interviews and field visits. Farmer interviews and field visits were conducted at Claveria, Misamis Oriental to gather additional data regarding costs and returns associated with animal production, and also to obtain the farm gate price of napier. Predicted biophysical results produced by the SCUAF model showed that application of napier grass cuttings, back into the system, directly as mulch or indirectly as animal manure resulted in higher maize and napier yields, lower soil erosion rates, and less of a reduction in soil mineral nitrogen and soil labile carbon levels. The comparison was made with the case where napier was sold off-farm as fodder. Cost-benefit analysis showed that higher economic returns were gained when napier grass cuttings were fed to animals, rather than applied directly as mulch. This economic benefit was attributed to the draught and transport services rendered by the animal and to a change in the value of the animals. This analysis highlights the value of utilizing animals within a napier grass strip system for control of erosion.
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  • 28
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    Integrated pest management reviews 3 (1998), S. 177-188 
    ISSN: 1572-9745
    Keywords: Cercospora zeae-maydis ; control ; grey leaf spot ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Grey leaf spot of maize, (Zea maydis L.) (Cercospora zeae-maydis) Tehon and Daniels, is one of the most destructive leaf diseases of maize. The distribution and severity of grey leaf spot has increased over the past 10 to 15 years. The United States and South Africa are main areas where research on the disease has been concentrated. The research results have provided valuable and significant insight into pathogen epidemiology and allowed an integrated management system to be developed. However, management options are a result of the agricultural system under which maize is produced. The pathogen survives only on maize, so crop rotation and stubble management are major factors in disease management, but resistant hybrids offer the best option for economic control. Many other factors, such as soil fertility, plant density, irrigation and chemical control, can significantly influence a grey leaf spot epidemic, although a single management practice will not control the pathogen effectively.
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  • 29
    ISSN: 1573-5028
    Keywords: cell cycle ; cis-elements ; histone promoter ; maize ; meristems ; transgenic Arabidopsis
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    Topics: Biology
    Notes: Abstract A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the ß-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant histone promoters, but unexplained activity also occurred at branching points of both stems and roots. Deletion of the upstream 558 bp of the promoter reduced its activity to an almost basal expression. Internal deletion of a downstream fragment containing plant histone-specific sequence motifs reduced the promoter activity in all tissues and abolished the expression in meristems. Thus, both the proximal and distal regions of the promoter appear necessary to achieve the final expression pattern in dicotyledonous plant tissues. In mesophyll protoplasts isolated from the transformed Arabidopsis plants, the full-length promoter showed both S phase-dependent and -independent activity, like other plant histone gene promoters. Neither of the 5′-truncated nor the internal-deleted promoters were able to direct S phase-dependent activity, thus revealing necessary cooperation between the proximal and distal parts of the promoter to achieve cell cycle-regulated expression. The involvement of the different regions of the promoter in the different types of expression is discussed.
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  • 30
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    Plant molecular biology 37 (1998), S. 749-761 
    ISSN: 1573-5028
    Keywords: development ; disease ; endosperm ; kinase ; maize ; receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe the isolation and characterization of maize cDNAs that are transcribed from a small gene family and encode a novel group of receptor-like kinases (RLKs). The distinctive extracellular domain of these novel RLKs includes a unique number and arrangement of leucine-rich repeats (LRRs), a proline-rich region (PRR), a putative protein degradation target sequence (PEST), and a serine-rich region (SRR). The intracellular domain contains a putative serine/threonine protein kinase. To distinguish them from other reported RLKs, these novel RLKs were termed leucine-rich repeat transmembrane protein kinases (LTKs). Based on analysis of available deduced protein sequences, LTK1 and LTK2 were predicted to be 92.1% identical, while LTK2 and LTK3 were predicted to be 97.5% identical. Though the three LTK proteins showed high homology, the region that most distinguished LTK1 from LTK2 and LTK3 was found in the extracellular domain, in the SRR. To differentiate between expression of the individual ltk genes, we used the reverse transcriptase polymerase chain reaction (RT-PCR) in combination with restriction enzyme analysis. While ltk1 transcripts were constantly present in all tissues tested, ltk2 and ltk3 transcripts were only detected in the endosperm. Furthermore, transcript levels for both ltk1 and ltk2 showed modulation during endosperm development, peaking at 20 days after pollination. These results suggest that members of the ltk gene family mediate signals associated with seed development and maturation.
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  • 31
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    Plant molecular biology 37 (1998), S. 621-628 
    ISSN: 1573-5028
    Keywords: barley ; development ; maize ; telomerase ; TRAP assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eukaryotic chromosomes terminate with specialized structures called telomeres. Maintenance of chromosomal ends in most eukaryotes studied to date requires a specialized enzyme, telomerase. Telomerase has been shown to be developmentally regulated in man and a few other multicellular organisms, while it is constitutively expressed in unicellular eukaryotes. Recently, we demonstrated telomerase activity in plant extracts using the PCR-based TRAP (Telomeric Repeat Amplification Protocol) assay developed for human cells. Here we report telomerase activities in two grass species, barley and maize, using a modified, semi-quantitative TRAP assay. Telomerase was highly active in very young immature embryos and gradually declined during embryo development. The endosperm telomerase activity was detectable, but significantly lower than in the embryo and declined during kernel development with no detectable activity in later stages. Telomerase activity in dissected maize embryo axis was several orders of magnitude higher than in the scutellum. Telomerase activity was not detected in a range of differentiated tissues including those with active meristems such as root tips as well as the internode and leaf base. The role of telomerase repression during differentiation and the relationship between chromosome healing and telomerase activity is discussed.
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  • 32
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    Plant molecular biology 36 (1998), S. 593-599 
    ISSN: 1573-5028
    Keywords: C4 photosynthesis ; gene family ; maize ; RbcS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RbcS genes exist as multigene families in most plant species examined. In this paper, we report an investigation into the expression patterns of two maize RbcS genes, designated in this report as RbcS1and RbcS2. We present the sequence of RbcS2 and show that the structure of the gene has several features in common with other monocot RbcS genes. To determine whether RbcS1 and RbcS2 fulfil different functional roles with respect to the C3 and C4 carbon fixation pathways, we have investigated the expression patterns of the two genes in different maize tissue types. Transcripts of both genes are found at high levels specifically in bundle-sheath cells of maize seedling leaves, indicating that both genes are expressed in the C4-type pattern. However, we show that RbcS1 transcripts are relatively more abundant than RbcS2 transcripts in C3 tissues such as husk leaves. These results are discussed with respect to the evolution of C4 carbon fixation and the mechanisms required for the cell-specific expression of RbcS genes.
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  • 33
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    Plant molecular biology 37 (1998), S. 121-129 
    ISSN: 1573-5028
    Keywords: cell cycle ; complementation ; cyclins ; maize ; root meristem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic cyclins. Complementation studies revealed that cycZm2w was able to rescue a budding yeast cyclin-deficient mutant (BF305–15d#21). As expected, cycZm2w is expressed in organs of the maize plant that possess meristematic activity, but is especially prominent in the proliferating regions of the root apex.
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  • 34
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    European journal of plant pathology 104 (1998), S. 611-617 
    ISSN: 1573-8469
    Keywords: Exserohilum turcicum ; Johnson grass ; maize ; northern corn leaf blight ; population genetic structure ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Setosphaeria turcica is the causal agent of northern corn leaf blight, a foliar maize disease of worldwide economic importance. In Europe, its severity increases. To investigate the pathogen's population-genetic structure in central Europe, a total of 80 isolates was sampled in Germany, Switzerland, France, Austria, and Hungary and investigated with 52 random amplified polymorphic DNA (RAPD) markers. The mating type of the isolates was determined in testcrosses. Among the 73 isolates from maize there were 26 different RAPD haplotypes. All isolates with identical haplotype are considered clonemates. The haplotype shared by most members was represented by 22 isolates from Germany, Switzerland, and France, indicating high fitness and substantial migration. Only a single clone had members in both southeastern Austria and southwestern Switzerland, suggesting that the Alps constitute a major barrier for this pathogen. Several haplotypes differed by only one or two RAPD bands from the predominant haplotype and may have arisen by mutation. Few other clonal lineages were detected. The evolution of some haplotypes could not be explained by mutation alone. Sexual recombination may rarely occur. In population samples from Germany, Switzerland, and France, mating type MAT2 was predominating, while most isolates from Austria and Hungary had MAT1. Seven isolates from Johnson grass (Sorghum halepense), an alternative host of S. turcica, were clonemates and very different in RAPD haplotypes from all isolates collected from maize.
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  • 35
    ISSN: 1573-5028
    Keywords: Dehydrin ; LEA D11 ; maize ; dhn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dehydrins (LEA D11 proteins) are the products of multigene families in a number of higher plants [5]. To date, however, only one dehydrin locus, dhn1 (a major embryo and drought-induced protein of ca. 18 kDa) has been placed on chromosome 6L of the genetic linkage map of maize. The presence of a larger, ca. 40 kDa embryo protein that is also specifically detected by anti-dehydrin antibodies had been observed in some maize inbreds, including B73, suggesting that other dhn loci may exist. The ca. 22 kDa and ca. 40 kDa immunopositive proteins were purified from B73 and their amino acid compositions determined. The two proteins' amino acid compositions are typical of dehydrins, yet they differ from each other, indicating that they are distinct dhn gene products. Different size alleles for both proteins, or presence/absence in the case of the ca. 40 kDa protein, were evident from comparisons of embryo proteins of various maize inbreds. Analysis of segregating F2 progeny derived from self-pollination of F1 hybrids from four crosses (B73 × OH43, Mo17 × A632, AHO × A632, Latente × A632) revealed that alleles of the two genes assort independently. Map positions of the two dhn loci were then determined using two maize recombinant inbred line (RIL) mapping populations. The predicted map position of the gene controlling production of the ca. 22 kDa protein confirmed that this protein is the product of the dhn1 gene. The gene encoding the ca. 40 kDa dehydrin-like protein maps to a new locus on chromosome 9S near wx1, which we have named dhn2.
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  • 36
    ISSN: 1573-5028
    Keywords: Cinnamoyl-CoA Reductase ; cDNA sequences ; lignins ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cinnamoyl-CoA Reductase (CCR, EC 1.2.1.44) catalyses the first step of the lignin pathway. Two full-length cDNAs identified by sequence analysis as CCR-encoding cDNAs were isolated from a maize root cDNA library. These two cDNAs designated ZmCCR1 and ZmCCR2 exhibit 73% sequence conservation at the nucleotide level for their coding regions and are relatively divergent at their 5′- and 3′-untranslated regions. They both contain a common signature which is thought to be involved in the catalytic site of CCR. Northern blot analysis indicated that ZmCCR2 was expressed at very low levels in roots whereas ZmCCR1 was widely expressed in different organs. The high level of ZmCCR1 gene expression along the stalk suggests that the corresponding enzyme is probably involved in constitutive lignification.
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  • 37
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    Plant and soil 198 (1998), S. 185-192 
    ISSN: 1573-5036
    Keywords: colonization ; crop-rotation ; maize ; mycorrhiza ; phosphorus ; tillage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We selected three crop production practices; crop rotation, tillage and phosphorus fertilization, all known to affect arbuscular mycorrhiza (AM) development, to study early AM intraradical colonization in maize. Half of the plots were planted during the first year with either a host (maize, Zea mays L.) or a non-host (canola, Brassica napus L.) crop, and all of them with maize for the second year. Tillage and P fertilization treatments were applied to the plots in the second year. Mycorrhiza development in maize was measured in pot culture bioassays conducted before planting and after harvest of the previous and the subsequent crops, and in the field during the second crop season. Previous cropping of a soil with canola (Brassica napus L.), a non-host plant species, delayed mycorrhiza development of maize in a bioassay conducted with that soil in comparison with a previous cropping cycle with maize (Zea mays L.) or with the original plant species in the field site, bromegrass (Bromus inermis Leys.) and alfalfa (Medicago sativa L.). The delay in mycorrhiza development after cropping with canola was also observed in samples taken from the field and in a bioassay, both conducted at the beginning of the subsequent cropping cycle. Tillage had, on average, little effect on intraradical colonization either in the field or in the bioassays. Phosphorus fertilization also had little effect on mycorrhiza development in the field. Crop rotation with a non-host had the strongest effect on intraradical mycorrhiza development of the three practices studied.
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  • 38
    ISSN: 1573-5036
    Keywords: intercrops ; maize ; model ; roots ; sorghum ; water transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A knowledge of plant interactions above and below ground with respect to water is essential to understand the performance of intercrop systems. In this study, a physically based framework is proposed to analyse the competition for soil water in the case of intercropped plants. A radiative transfer model, associated with a transpiration-partitioning model based on a modified form of the Penman-Monteith equation, was used to estimate the evaporative demand of maize (Zea mays L.) and sorghum ( Sorghum vulgare R.) intercrops. In order to model soil–root water transport, the root water potential of each species was calculated so as to minimise the difference between the evaporative demand and the amount of water taken up by each species. A characterisation of the micrometeorological conditions (net radiation, photosynthetically active radiation, air temperature and humidity, rain), plant water relations (leaf area index, leaf water potential, stomatal conductance, sap flow measurements), as well as the two-component root systems and water balance (soil–root impacts, soil evaporation) was carried out during a 7-day experiment with densities of about 4.2 plant m-2 for both maize and sorghum. Comparison of the measured and calculated transpiration values shows that the slopes of the measured versus predicted regression lines for hourly transpiration were 0.823 and 0.778 for maize and sorghum, respectively. Overall trends in the variation of volumetric water content profiles are also reasonably well described. This model could be useful for analysing competition where several root systems are present under various environmental conditions.
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  • 39
    ISSN: 1573-5036
    Keywords: available soil water ; extraction pattern ; maize ; sorghum ; soya bean ; sunflower ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The estimation of soil water reserves is essential for irrigation management. The usual way of calculating these reserves, held between the soil moisture content at field capacity and the classical limit of −1.5 MPa considered as the lower limit of available water, over the rooting depth of the crop, does not correspond with the real behaviour of crops as regards their ability to extract soil water and should be only considered as the apparent available water (AAW). Measurements of moisture profiles made using a neutron probe soil moisture meter from 1970 until 1991 on unirrigated crops at the INRA Agronomy Station at Toulouse-Auzeville, France, on a deep silty clay soil with a high water holding capacity have enabled us to define the water extraction capacities of maize ( Zea mays L.), sunflower (Helianthus annuus L.), sorghum (Sorghum bicolor L. Moench), soya bean (Glycine max L. Merr.), and winter wheat (Triticum aestivum L.). The results show, not only that all the crops can extract soil water from beyond −1.5 MPa in the surface layers to varying degrees and depths, depending on the crop, but also that deeper down, AAW is not fully used, as the moisture profile gradually returns to field capacity. Of the five crops studied, maize extracts the most water from the top 0.5 m, removing 150% of AAW. This amount falls rapidly lower down, reaching nil at 1.6 m. Conversely sunflower extracts less near the surface, but uses all AAW up to 1.2 m, and still extracts 85% of AAW at 1.6 m. Sorghum is somewhat comparable to sunflower, but with a lower use over the entire profile. Soya bean exhibits strong extraction to 1.0 m, and then much less at depth. As to wheat, its extraction capability is quite high near the surface, and then falls steadily with depth where it is still 30% of AAW at 1.6 m. Soil moisture measurements realised on a bare soil during several successive years were used to fix the maximum soil evaporation and to suggest the contribution of crops in soil water depletion from uppermost layers. The water extraction capacities have been modelled and introduced into the model EPICphase, a modified version of the model EPIC, adapted for irrigation management. Four parameters have been introduced to simulate: (1) the rooting pattern of the crop (parameter α), (2) the degree of involvement of deep layers (parameter p), (3) the fraction of AAW beyond which crop transpiration is affected (parameter t) and (4) the intensity of extraction beyond the limit of −1.5 MPa as a function of soil depth (parameter d). Calibrated on the basis of the driest year since 1970 for each crop, the model was then validated under unirrigated conditions, and then tested on irrigated maize plots. Under unirrigated conditions, the simulations correctly reproduced the water extraction by the five crops, both in an extremely dry year and in a wet year. The observed differences between simulations and observations were found mostly at about 0.1 m depth, and were due to lack of precision of moisture measurements with the neutron probe. From 0.2 to 0.6 m the simulations have a tendency to overestimate the extraction. These differences are explained by water fluxes which are especially high in these layers because of the processes of evaporation from the soil and plant transpiration, which are difficult to simulate with precision. Below 0.6 m, a more stable zone where water movements are of minor importance, the simulations are very precise. For irrigated maize, the results show a very good fit between simulation and measurement, indicating that these water extraction capacity figures could be used for irrigation management provided that the rules for exploitation of the water reserves are well established.
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  • 40
    ISSN: 1573-5036
    Keywords: Grevillea robusta ; maize ; root length ; root biomass ; root sampling ; sieves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The purpose of this study was to investigate the effects of different mesh sizes on the recovery of root length and biomass and to determine whether the degree of recovery was influenced by plant species and sample location. Sieves of 2.0, 1.0, 0.5 and 0.25 mm (4.0, 1.0, 0.25 and 0.06 mm2) mesh sizes were used to recover and measure the root length and biomass of Zea mays L. (maize) at 0–15 cm and 30–45 cm depths and of Grevillea robusta A. Cunn. ex R. Br. (grevillea) at the same depths 1.0 m and 4.5 m from a line of grevillea trees. At 0–15 cm, the coarser sieves (sum collected with 2.0 and 1.0 mm sieves) recovered approximately 80% of the total root biomass measured, but only 60% of the root length. The proportion of total maize root length and biomass recovered by the coarser sieves decreased with soil depth. The proportion of total grevillea root length recovered by the coarser sieves was similar at the two soil depths, but increased slightly with distance from the tree line. The ≥ 0.5 mm sieves recovered between 93 and 96% of grevillea and maize root biomass and between 73 and 98% of their root length, depending on the sample location. Roots passing through the 0.5 mm sieve, but recovered by the 0.25 mm sieve were about 20% of total maize root length and grevillea root length at 1.0 m from the tree line but 〈 5% of the total grevillea root length at 4.5 m from the tree. Roots passing through the 0.5 mm sieve but recovered by the 0.25 mm sieve contributed only slightly to root biomass. Although the ≥ 0.5 mm sieves provided adequate measurements of root biomass, the ≥ 0.25 mm sieves were required for accurate measurement of fine root length. There was no universal correction for root length and biomass underestimation when large sieve sizes were used because the proportions of length and biomass recovered depended on the plant species and on soil depth and distance from the plant.
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  • 41
    ISSN: 1573-5036
    Keywords: acetylene inhibition ; denitrification ; irrigated field ; maize ; 15N balance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Denitrification and total N losses were quantified from an irrigated field cropped to maize and wheat, each receiving urea at 100 kg N ha-1. During the maize growing season (60 days), the denitrification loss measured directly by acetylene inhibition-soil cover method amounted 2.72 kg N ha-1 whereas total N loss measured by 15N balance was 39 kg ha-1. Most (87%) of the denitrification loss under maize occurred during the first two irrigation cycles. During the wheat growing season (150 days), the denitrification loss directly measured by acetylene inhibition-soil cover and acetylene inhibition-soil core methods was 1.14 and 3.39 kg N ha-1, respectively in contrast to 33 kg N ha-1 loss measured by 15N balance. Most (70-88%) of the denitrification loss under wheat occurred during the first three irrigation cycles. Soil moisture and NO 3 - -N were the major factors limiting denitrification under both crops. Higher N losses measured by 15N balance than C2H2 inhibition method were perhaps due to underestimation of denitrification by C2H2 inhibition method and losses other than denitrification, most probably NH3 volatilization.
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  • 42
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    Euphytica 104 (1998), S. 17-23 
    ISSN: 1573-5060
    Keywords: crossability ; D genome ; durum wheat ; embryo culture ; haploid ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract With the aim of examining crossability of durum wheat with maize, two sets of durum wheat genotypes and a set of D-genome chromosome substitution lines of the durum wheat variety ‘Langdon’ were crossed with maize, and followed by 2,4-dichlorophenoxyacetic acid (2,4-D) treatment in detached-tiller culture. In crosses of 25 durum wheat genotypes (breeding lines) with maize, percent frequencies of embryo formation increased from 1.4% to 2.8% by adding silver nitrate to the detached-tiller culture solution. In crosses of 32 durum wheat genotypes (advanced lines and varieties) with maize using the silver nitrate addition, frequencies of embryo formation ranged from 0.0% to 15.8%; seven genotypes showing more than 6.0% embryo formation frequency were related in their pedigrees. In crosses of a set of chromosome substitution lines with maize, higher frequencies of embryo formation were obtained in substitution lines with chromosomes 1D, 3D, 4D and 7D. These results suggest that 1) adding silver nitrate to the 2,4-D treatment increases overall frequency of embryo formation but is not effective enough to induce the development of seeds and embryos from all durum wheat genotypes, and 2) some D-genome chromosomes substituted in a durum wheat genetic background may enhance crossability with maize in combination with homoeologous chromosomes of durum wheat.
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  • 43
    ISSN: 1573-1561
    Keywords: Sap beetle ; attraction ; yeast ; volatile ; headspace ; fermentation ; glucose ; maltose ; sucrose ; maize ; sweet corn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Attraction to microbial volatiles was examined for the sap beetle, Carpophilus humeralis, which is a pest of maize. Using 54 pure yeast and bacterial cultures, we evaluated differences in volatile emissions among species of microorganisms and whether these differences were associated with insect attraction. On a sterile corn-based medium, both yeasts and bacteria generally multiplied well and produced detectable volatile metabolites. The yeasts ranged from inactive to highly attractive, but no bacterial cultures attracted beetles above control levels. A variety of alcohols, esters, ketones, acids, and phenolic compounds were identified from the headspace above yeast cultures. Growth, volatile production, and, ultimately, attractiveness to beetles depend strongly on the ability of the yeasts to assimilate and/or ferment the carbohydrates present. Abundant volatile production on sweet corn was observed only with yeasts that are able to ferment sucrose and/or maltrose. Saccharomyces cerevisiae (ferments glucose, sucrose, and maltose) and Candida shehatae (ferments glucose and maltose) produced considerably more attractive volatiles than Candida guilliermondii, which only ferments glucose. Yeast volatiles important for beetle attraction included typical fermentation-associated substances (ethanol, acetaldehyde, 2-methyl-1-propanol, 1-propanol, ethyl acetate, 3-methyl-1-butanol and 2-methyl-1-butanol), and also 3-hydroxy-2-butanone, whose presence was not correlated with the occurrence of fermentation. Using aqueous mixtures of synthetic components that produced headspace compositions simulating those of attractive yeasts, it was shown that the typical fermentation volatiles are attractive but not essential for attractiveness. 3-Hydroxyl-2-butanone is sufficient but not necessary, although its attractiveness is enhanced by the presence of fermentation volatiles such as ethanol and 2-methyl-1-proponol. In nature, the beetles could take advantage of a variety of different microbial metabolic processes to locate hosts. The laboratory bioaasays in this study involved flight and therefore were particularly relevant to host-finding behavior in the field.
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  • 44
    ISSN: 1572-9680
    Keywords: alley-cropping ; credit ; erosion ; labour ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Considerable resources have been expended promoting hedgerow intercropping with shrub legumes to farmers in the Philippine uplands. Despite the resources committed to research and extension, persistent adoption by farmers has been limited to low cost versions of the technology including natural vegetation and grass strips. In this paper, cost-benefit analysis is used to compare the economic returns from traditional open-field maize farming with returns from intercropping maize between leguminous shrub hedgerows, natural vegetation strips and grass strips. An erosion/productivity model, Soil Changes Under Agroforestry, was used to predict the effect of erosion on maize yields. Key informant surveys with experienced maize farmers were used to derive production budgets for the alternative farming methods. The economic incentives revealed by the cost-benefit analysis help to explain the adoption of maize farming methods in the Philippine uplands. Open-field farming without hedgerows has been by far the most popular method of maize production, often with two or more fields cropped in rotation. There is little persistent adoption of hedgerow intercropping with shrub legumes because sustained maize yields are not realised rapidly enough to compensate farmers for establishment and maintenance costs. Natural vegetation and grass strips are more attractive to farmers because of lower establishment costs, and provide intermediate steps to adoption. Rural finance, commodity pricing and agrarian reform policies influence the incentives for maize farmers in the Philippine uplands to adopt and maintain hedgerow intercropping.
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  • 45
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    Agroforestry systems 41 (1998), S. 167-179 
    ISSN: 1572-9680
    Keywords: maize ; sample size ; sampling strategy ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Data from a Brazilian Inga/maize/bean alleycrop experiment are used to illustrate patterns of variation between and within crop rows and their possible effects in the determination of a sampling strategy. Three methods for the determination of sample sizes in agroforestry experiments are compared: the combined plant technique, the formula of Snedecor and Cochran and variance component estimation. Results show that variability in the edge rows was generally greater than variability in the inner rows and suggest three sampling strata – one for each of the two edge rows and the third for the inner rows. For all three sampling methods a sample size greater than ten plants per stratum per block gave little extra precision. Increasing the number of blocks improves precision and may permit a smaller sample size per row to achieve the same precision. The comparison of different methods of sample size determination shows that variance component estimation is the most flexible and efficient approach.
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  • 46
    ISSN: 1573-5028
    Keywords: in situ hybridization ; maize ; promoter analysis ; tobacco transformation ; tubulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the past few years many α- and β-tubulin genes of different organisms have been cloned and studied, and in most systems studied they constitute multigene families. In plants, most studies have been done in Arabidopsis thaliana and Zea mays. In this paper, the study of mRNA accumulation by in situ hybridization and the activity of three maize α-tubulin gene promoters (tua1, tua2 and tua3) in transgenic tobacco plants are described. In maize, the expression of these three tubulin isotypes differ in the root and shoot apex and is associated with different groups of cells throughout the distinct stages of cell differentiation. In transgenic tobacco plants the promoters of the genes, fused to the uidA reporter gene (GUS), direct expression to the same tissues observed by in situ hybridization experiments. The tua1 promoter is mainly active in cortex-producing meristematic cells and in pollen, whereas tua3 is active in cells which are differentiating to form vascular bundles in the root and shoot apices. The accumulation of tua2 mRNA is detected by RNA blot in a similar form as tua1, but at a very much low level. In situ hybridization indicates that the tua2 mRNA specifically accumulates in the maize root epidermis. No GUS staining was detected in transgenic tobacco plants with the tua2 promoter. The difference in expression of the specific genes may be linked to processes where microtubules have different functions, suggesting that in plants, as in animals, there are differences in the function of the tubulin isotypes.
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  • 47
    ISSN: 1573-5028
    Keywords: maize ; pollen ; pollen-specific promoter ; transgenic plants ; Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The region of the promoter of the pollen-specific maize gene, ZM13, from -119 to -37 was analyzed by a linker-scanning type of substitution mutagenesis and two areas were shown to be responsible for pollen expression: a proximal region delineated by mutations from -84 to -53 that conferred pollen specificity, and an upstream region delineated by a mutation from -107 to -102 (Q-element) that could increase the expression of the proximal region but showed no ability to cause expression in pollen on its own. Replacement of both of these areas with other sequences including the CaMV 35S promoter failed to replace activity.
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  • 48
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    Plant and soil 199 (1998), S. 283-291 
    ISSN: 1573-5036
    Keywords: dry matter partitioning ; maize ; 15N ; nitrogen uptake ; nitrogen use efficiency ; senescence ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In eastern Canada, the use of fertilizer N has been identified as the most energy-consuming component of maize (Zea mays L.) grain production. As the economic and environmental costs of excessive N fertilization rise, there is an increased emphasis on selection of hybrids with greater N use efficiency (NUE; defined as the ratio of the amount of 15N recovered in grain or stover dry matter to the amount of fertilizer 15N applied to the soil in this study). Using an 15N-labelling approach, a field study was conducted on a tile-drained Brandon loam soil (Typic Endoaquoll) on the Central Experimental Farm at Ottawa, Canada (45°22′ N, 75°43′ W) in 1993 and 1994. Fertilizer N uptake and partitioning within the plant in relation to dry matter changes were monitored during development of a current stay-green maize hybrid and an older early-senescing hybrid grown with three fertilizer N levels (0, 100, 200 kg N ha-1). Dry matter, N concentration and15 N atom% enrichment of plant components were determined at five growth stages. The current stay-green hybrid, ‘Pioneer 3902’ had greater NUE than the old early-senescing hybrid, ‘Pride 5’, which was associated with 24% more dry matter production and 20% more N uptake during grain fill for Pioneer 3902. There was no indication of greater allocation of N to the grain in Pioneer 3902. Our data suggest that prolonged maintenance of green leaf area for photosynthate production during grain fill and the ability to take up available soil N later in grain filling are characteristics of maize hybrids with greater NUE.
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  • 49
    ISSN: 1573-5036
    Keywords: carbon-13 ; maize ; modelling ; physical protection ; stablecarbon isotope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cultivation of forest and grassland soils induces heavy changes in soil organic matter (SOM) dynamics. To better predict the effect of cultivation, there is a need to describe which organic pools are affected and to which extent. We used a chronosequence of thick humic forest soils converted to maize cultivation for 40 yr in southwest France. The dynamics of soil carbon was investigated through particle-size fractionation and the use of 13C allowed to distinguish forest-derived organic matter and new crop-derived organic matter. This partitioning of soil carbon by size on one hand and by age on the other provided a precise description of carbon turnover. The level towards which tend the organic pools under cultivation showed that the decay rates of soil carbon were one order of magnitude higher under cultivation than under forest. SOM can thus be considered as deprotected under cultivation. All size fractions appeared to be deprotected to the same extent. A progressive transfer of silt-sized C to clay-sized C was nevertheless suspected and attributed to the decreasing stability of fine silt-sized microaggregates with cultivation. SOM furthermore contained some very stable C present as silt-sized and possibly clay-sized particles. The turnover times of maize-derived organic matter was the same as that observed in similar soils cultivated for centuries. This indicated that the new conditions induced by cultivation were reached in the very first years after forest clearing and that the high initial SOM content and high mineralization rate of initial organic matter did not affect the dynamics of newly incorporated carbon.
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  • 50
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    Plant and soil 199 (1998), S. 177-186 
    ISSN: 1573-5036
    Keywords: colonization ; crop rotation ; harvest index ; maize ; mycorrhizae ; phosphorus ; tillage ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We conducted a field experiment to test the hypothesis that improved phosphorus nutrition occurs in maize plants with rapid arbuscular (AM) mycorrhizae development at early developmental stages and that this also is reflected in dry matter allocation and final yield. A split-split plot design was used with previous crop (Zea mays L.-maize and Brassica napus L.-canola), tillage practices (no-tillage or conventional tillage) and P fertilization (5 levels) as factors chosen to modify mycorrhizae development at early developmental stages of maize. Previous cropping with canola resulted in decreased shoot-P concentration and shoot growth of maize at early stages. No-tillage resulted in higher shoot-P concentration but lower shoot weight than conventional tillage. Greater shoot-P uptake was related to a rapid intraradical development of mycorrhizae (previous crop of maize) or rapid connection to a mycorrhizal mycelium network (no-tillage treatments). Maize yield and harvest index were lower after cropping with canola. The yield for conventional tillage was higher than that for no-tillage but the harvest index was lower. The hypothesis was supported at early stages of maize growth by the effect of previous crop but not by results of tillage, because an unknown factor reduced growth in the no-tillage system. The hypothesis was supported at maturity by increased biomass allocation to grain relative to total shoot weight in treatments with greater shoot-P concentration at early stages.
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  • 51
    ISSN: 1573-5036
    Keywords: image processing ; maize ; plant residue ; root diameter ; root length ; root washing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Historically, destructive root sampling has been labor intensive and requires manual separation of extraneous organic debris recovered along with the hydropneumatic elutriation method of separating plant roots from soils. Quantification of root system demographics by public domain National Institute of Health (NIH-Image) and Root Image Processing Laboratory (RIPL) image processing algorithms has eliminated much of the labor-intensive manual separation. This was accomplished by determining the best length to diameter ratio for each object during image analyses. Objects with a length to diameter ratio less than a given threshold are considered non-root materials and are rejected automatically by computer algorithms. Iterative analyses of length to diameter ratios showed that a 15:1 ratio was best for separating images of maize (Zea mays L.) roots from associated organic debris. Using this threshold ratio for a set of 24 soil cores, a highly significant correlation (r2 = 0.89) was obtained between computer image processed total root length per core and actual root length. A linear relationship (r2 = 0.80) was observed between root lengths determined by NIH-Image analyses and lengths determined independently by the RIPL imaging system, using the same maize root + debris samples. This correlation demonstrates that computer image processing provides opportunities for comparing root length parameters between different laboratories for samples containing debris.
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  • 52
    ISSN: 1573-5036
    Keywords: maize ; model ; root architecture ; root intersection ; root length ; stochastic geometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The spatial distribution of root length density (RLD) is important because it affects water and nutrient uptake. It is difficult to obtain reliable estimates of RLD because root systems are very variable and heterogeneous. We identified systematic trends, clustering, and anisotropy as geometrical properties of root systems, and studied their consequences for the sampling and observation of roots. We determined the degree of clustering by comparing the coefficient of variation of a simulated root system with that of a Boolean model. We also present an alternative theoretical derivation of the relation between RLD and root intersection density (RID) based on the theory of random processes of fibres. We show how systematic trends, clustering and anisotropy affect the theoretical relation between RLD and RID, and the consequences this has for measurement of RID in the field. We simulated the root systems of one hundred maize crops grown for a thermal time of 600 K d, and analysed the distribution of RLD and root intersection density RID on regular grids of locations throughout the simulated root systems. Systematic trends were most important in the surface layers, decreasing with depth. Clustering and anisotropy both increased with depth. Roots at depth had a bimodal distribution of root orientation, causing changes in the ratio of RLD/RID. The close proximity of the emerging lateral roots and the parent axis caused clustering which increased the coefficient of variation.
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  • 53
    ISSN: 1573-5060
    Keywords: interspecific cross ; haploid ; wheat ; maize ; rye chromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genotypic influence of both male and female parents on haploid production through interspecific crosses was studied using eight wheat and four maize genotypes. The average numbers of embryos and green haploid plantlets obtained per pollinated floret were 17.6% and 10.1%, respectively. Clear genotypic influence of the wheat genotype was detected, but heterozygosity of the wheat did not affect haploid production. Analogous response to anther culture and interspecific crossing was observed, still a wheat variety which did not respond to anther culture, produced 1.1 plantlets per pollinated spike upon maize pollination. This appears to be a major advantage of interspecific crossing compared to anther culture technique in wheat. Circumstantial evidence is presented for specific wheat × maize interaction on haploid plantlet formation. Rye chromatin enhanced haploid production but only in a complete 1B/1R substitution line. Ovaries with an embryo were found to be dispersed evenly all over the wheat spike, suggesting that within certain limits the developmental stage of ovaries and thus time of pollination within a spike are not as important as it was previously assumed.
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  • 54
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    Plant molecular biology 37 (1998), S. 483-493 
    ISSN: 1573-5028
    Keywords: maize ; transposon tagging ; embryo ; endosperm ; development ; dsc1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recessive mutations in the maize dsc1 locus prevent normal kernel development. Solidification of the endosperm in homozygous dsc1− mutant kernels was undetectable 12 days after pollination, at which time the tissue was apparently completely solidified in wild-type kernels. At later times endosperm did solidify in homozygous dsc1− mutant kernels, but there was a marked reduction in the volume of the tissue. Embryo growth in homozygous dsc1− kernels was delayed compared to wild-type kernels, but proceeded to an apparently normal stage 1 in which the scutellum, coleoptile, and shoot apex were clearly defined. Embryo growth then ceased and the embryonic tissues degraded. Late in kernel development no tissue distinctions were obvious in dsc1− mutant embryos. Immature mutant embryos germinated when transplanted from kernels to tissue culture medium prior to embryonic degeneration, but only coleoptile proliferation was observed. The dsc1 gene was isolated by transposon tagging. Analysis of the two different dsc1− mutations confirmed that transposon insertion into the cloned genomic locus was responsible for the observed phenotype. Dsc1 mRNA was detected specifically in kernels 5–7 days after pollination. These data indicate Dsc1 function is required for progression of embryo development beyond a specific stage, and also is required for endosperm development.
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  • 55
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    European journal of plant pathology 104 (1998), S. 301-311 
    ISSN: 1573-8469
    Keywords: diagnosis ; fungi ; nucleic acid hybridization ; maize ; polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The fungus Fusarium moniliforme infects a wide range of crops throughout the world. In maize (Zea mays L.) it causes seedling blight and root, stalk, and ear rots. A simple procedure that can be used to detect infection by F. moliliforme from infected plant tissues has been developed. A F. moniliforme genomic library was prepared and used to identify the recombinant clones containing fungal DNA sequences not hybridizing with the DNA of the host plant, maize. Based on the nucleotide sequence information obtained from the F. moniliforme pUCF2 genomic clone, specific oligonucleotides were designed and used as primers for in vitro DNA amplification by the polymerase chain reaction. An amplification product was obtained with F. moniliforme DNA preparations whereas no amplified DNA was detected with DNAs from other fungal pathogens, including various Fusarium species, or from the host plant. This PCR analysis was successfully employed to identify F. moniliforme directly from the mycelia that develop from naturally infected maize seeds, with no need to obtain pure fungal cultures for reliable diagnosis. The protocol can be used for the diagnosis of infected plants and soils in epidemiological studies of Fusarium diseases, for seed health testing, and for evaluation of susceptibility to colonization in commercial maize hybrids.
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  • 56
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    Biologia plantarum 41 (1998), S. 303-306 
    ISSN: 1573-8264
    Keywords: biochanin A ; luteolin ; maize ; naringenin ; quercetin ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Biochanin A, luteolin, naringenin and quercetin significantly decreased the percentage of root segments bearing the intraradical proliferating hyphae of Glomus fistulosum at the concentrations up to 10 µmol dm-3. The growth of hyphae was inhibited by biochanin A and luteolin whereas no significant effects of quercetin and naringenin were observed.
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  • 57
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    Mycopathologia 138 (1997), S. 77-89 
    ISSN: 1573-0832
    Keywords: Aflatoxin ; Aspergillus flavus ; Fusarium spp ; maize ; postharvest treatment ; preharvest contamination ; storage ; Costa Rica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Projects funded by International Development Research Centre (IDRC) of Canada and the European Commission have enabled the examination of more than 3000 samples of maize collected from all regions of Costa Rica at different stages, from the growing crop through storage to final sale, and at different water contents. Contamination with Aspergillus flavus was frequent and about 80% of samples contained more than 20 ng aflatoxins g-1 grain. Average contamination with aflatoxins in the Brunca Region was 〉 274 ng g -1 while that in other regions was 〈 70 ng g -1. Except in Brunca region, where it averaged 376 ng g -1, contamination of grain from commercial sources was slightly less than of that from farms (≤15 ng g-1). It appeared that samples kept on the cob after harvest contained almost no aflatoxin while shelled samples were frequently highly contaminated. Experiments were therefore done in Brunca and Huetar Atlantic Regions, utilising 34 experimental maize crops to study in detail the development of A. flavus and aflatoxin from before harvest, through postharvest treatment before drying and through storage for six months. A. flavus was isolated more frequently from maize shelled immediately after harvest than from that kept on the cob until it could be dried, and from more samples from the Brunca Region than from the Huetar Atlantic Region. Samples harvested with ≥18% water content often contained 〉70% of grains infected with A. flavus but sometimes there were few grains infected. As found in the initial survey, more aflatoxin contamination developed in shelled maize than in that handled on the cob during the period from harvesting to drying, especially if the delay was more than 5 days, and more in Brunca than in Huetar. Shelled grain contained 400–800 ng aflatoxin g -1 in Brunca but 〈100 ng g-1 in Huetar while grain kept on the cob contained 〈30 ng g-1, even with 〉18% water content. Incidence of Fusarium spp. exceeded 50% except where A. flavus colonized more than 80% of grains.
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  • 58
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    Mycopathologia 140 (1997), S. 149-155 
    ISSN: 1573-0832
    Keywords: α- and β-conidia ; carnation leaf agar (CLA) ; light ; maize ; Phaeocytostroma ambiguum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The formation of conidia in Phaeocytostroma ambiguum on different media and conditions was investigated in this study. Carnation leaf agar (CLA) and a 12 h photoperiod (24/18 °C) provided excellent conditions for the promotion of rapid formation of both alpha (α) and beta (β) conidia in a number of P. ambiguum isolates. The dimensions of α- and β-conidia amounted to 6.0–19.6 × 3.8–7.5 μm and 6.0–24.9 × 1.1–2.6 μm, respectively. They were produced on short or elongate, simple and branched conidiophores. β-conidia have not been described before in P. ambiguum. Intermediate conidia were rarely found.
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  • 59
    ISSN: 1573-0867
    Keywords: Africa ; biogenic emission ; dry season ; dynamic chamber ; forest ; grassland ; groundnut ; maize ; Miombo typesavanna ecosystem ; nitric oxide ; pulsing ; sorghum ; wet season ; Zimbabwe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract During October to December 1994, we measured diel and seasonal variability in nitric oxide (NO) exchange by a southern African savanna ecosystem (Grasslands Research Station, Marondera, Zimbabwe). A set of automated dynamic chambers was installed in three sub-sites: Miombo forest, natural grassland, and cropped soils (maize, sorghum, groundnut). The latter received a single application of commercialy available in-organic or cowdung fertilizer. The dynamic chamber system is described in detail, including correction of the resulting data for wall losses and gas-phase reactions of the NO-NO2-O3 triad. During the dry season, net vertical NO fluxes were close to the detection limit (0.44 ngN m-2s-1) regardless of vegetation type. During the transition from dry to wet season strong "pulsing" effects of the NO flux were observed: NO emissions increased by a factor of up to 60 within hours. During the wet season, NO emissions varied between the different sub-sites of the ecosystem; corresponding means are in ranking order of fertilized agricultural plots (27.2 ngN m-2 s-1), unfertilized agricultural plots (8.5 ngN m-2 s-1 ), grassland (4.4 ngN m-2 s-1 ), and Miombo forest (1.5 ngN m-2 s-1). Soil moisture was found to be the dominant factor controlling the NO fluxes. Whenever it was not limiting, the diel behaviour of NO emission followed the daily variation of soil temperature measured 1 cm below the surface.
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  • 60
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    Plant molecular biology reporter 15 (1997), S. 371-376 
    ISSN: 1572-9818
    Keywords: band shift assay ; DNA binding protein ; maize ; nuclear protein extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The analysis of DNA binding proteins can be difficult when only small quantities of tissue expressing the desired protein are available. We present a protocol for the preparation of nuclear extracts from as little as 100 mg of tissue. This protocol is well suited for extraction of DNA binding proteins from tissues that are difficult to obtain in large quantities such as maize embryos.
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  • 61
    ISSN: 1573-5028
    Keywords: DNA polymerases ; DNA primase ; maize ; seed germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three DNA polymerase activities, named 1, 2 and 3 were purified from maize embryo axes and were compared in terms of ion requirements, optimal pH, temperature and KCl for activity, response to specific inhibitors and use of templates. All three enzymes require a divalent cation for activity, but main differences were observed in sensitivity to inhibitors and template usage: while DNA polymerases 1 and 2 were inhibited by N-ethyl maleimide and aphidicolin, inhibitors of replicative-type enzymes, DNA polymerase 3 was only marginally or not affected at all. In contrast, DNA polymerase 3 was highly inhibited by very low concentrations of ddTTP, an inhibitor of repair-type enzymes, and a 100-fold higher concentration of the drug was needed to inhibit DNA polymerases 1 and 2. Additionally, DNA polymerases 1 and 2 used equally or more efficiently the synthetic template polydA-oligodT, as compared to activated DNA, while polymerase 3 used it very poorly. Whereas DNA polymerases 1 and 2 shared properties of replicative-type enzymes, DNA polymerase 3 could be a repair-type enzyme. Moreover, a DNA primase activity copurified with the 8000-fold purified DNA polymerase 2, strenghtening the suggestion that polymerase 2 is a replicative enzyme, of the α-type. This DNA primase activity was also partially characterized. The results are discussed in terms of relevant data about other plant DNA polymerases and primases reported in the literature.
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  • 62
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    Plant molecular biology 34 (1997), S. 383-392 
    ISSN: 1573-5028
    Keywords: maize ; mitochondrion ; RNA b ; RNA plasmids ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cytoplasm maize mitochondria. A soluble complex of RNA b, responsible for synthesis of the more abundant (+) RNA b strand in mitochondrial lysates, was identified. The complex had a buoyant density of 1.49 g/cm3, indicating a substantial non-nucleic acids content. The sedimentation coefficient of the complex, however, was only slightly larger than that of deproteinized RNA b. Synthesis of RNA b as well as the larger RNA plasmid, RNA a, was resistant to heparin, suggesting that, for both RNAs, preformed complexes between an RNA template and an RNA-dependent RNA polymerase capable of elongating in vivo preinitiated RNA plasmid strands, were present in the lysate. Only a small fraction of RNA b molecules were bound in the complex; the bulk of RNA b sedimented at the same rate as the deproteinized RNA. Thus, after replication, maize mitochondrial plasmids are not associated with nucleoprotein capsids although their synthesis takes place through ribonucleoprotein replication complexes.
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  • 63
    ISSN: 1573-5028
    Keywords: Mutator ; transposon ; maize ; antisense ; expression ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Activity of the Mutator transposons of Zea mays varies in different tissues and at different stages of development. In the soma, Mu elements excise at a high frequency late in tissue development. In germ cells, Mu elements rarely excise, but they amplify and insert at high levels around the time of meiosis. At all other times, Mu elements can duplicate and insert at a low frequency. To determine whether the patterns of Mutator activity correlate with tissue or cell-specific transcription of the regulatory transposon MuDR, we used in situ hybridization to localize the sense MuDR transcripts, mudrA and mudrB, in pistillate florets and embryos of four different maize Mutator stocks. We found mudrA and mudrB transcripts uniformly distributed in all tissues of immature, meristem-rich florets and in both somatic and germinal tissues of mature florets. In mature flowers, transcripts of both genes accumulate to high levels in the tapetal (endothelium) layer surrounding the embryo sac. We also found transcripts from the antisense strand of the mudrA gene in all cell types in the florets. In developing embryos, all MuDR transcripts were present in all tissues. Different Mutator stocks had characteristic accumulation patterns that were maintained throughout embryo development.
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  • 64
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    Transgenic research 6 (1997), S. 157-168 
    ISSN: 1573-9368
    Keywords: transgenic ; maize ; endosperm ; expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The tissue-specific, developmental, and genetic control of four endosperm-active genes was studied via expression of GUS reporter genes in transgenic maize plants. The transgenes included promoters from the maize granule-bound starch synthase (Waxy) gene (zmGBS), a maize 27 kDa zein gene (zmZ27), a rice small subunit ADP-glucose pyrophosphorylase gene (osAGP) and the rice glutelin 1 gene (osGT1). Most plants had a transgene expression profile similar to that of the endogenous gene: expression in the pollen and endosperm for the zmGBS transgene, and endosperm only for the others. Histological analysis indicated expression initiated at the periphery of the endosperm for zmGBS, zmZ27 and osGT1, while osAGP transgene activity tended to start in the lower portion of the seed. Transgene expression at the RNA level was proportional to GUS activity, and did not influence endogenous gene expression. Genetic analysis showed that there was a positive dosage response with most lines. Activity of the zmGBS transgene was threefold higher in a low starch (shrunken2) genetic background. This effect was not seen with zmZ27 or osGT1 transgenes. The expression of the transgenes is discussed relative to the known behaviour of the endogenous genes, and the developmental programme of the maize endosperm
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  • 65
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    Molecular breeding 3 (1997), S. 351-357 
    ISSN: 1572-9788
    Keywords: lignin ; bm3 ; maize ; O-methyltransferase ; PCR ; deletion ; brown midrib
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The caffeic acid O-methyltransferase (COMT) gene plays an important role in the synthesis of lignin. We have used the polymerase chain reaction in conjuction with genomic analysis to characterize deletion mutations of this gene in maize. In addition, we have analyzed and compared regions of the COMT gene from three distinct heterotic groups. Both PCR and Southern analysis indicate that the active wild-type COMT gene can be polymorphic. We suggest that the intron domain of at least one heterotic inbred can contribute to the alteration of the wild-type gene. In addition, multiple deletion mutations have occurred at this locus. We have found a previously uncharacterized deletion mutation in which segments of both the intron and exon have been deleted and replaced by other sequences. Precise knowledge of its sequence has allowed us to develop an assay by which we can follow this mutation in a breeding program.
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  • 66
    ISSN: 1572-9788
    Keywords: Alachlor ; herbicide tolerance ; maize ; RFLP ; SSR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to detect the genetic architecture of maize tolerance to Alachlor, a widely used chloroacetanilide, linkage analysis between the expression of the trait and allelic composition of molecular markers was performed. The experiment was carried out on a population of 142 recombinant inbred lines, developed starting from the F1 between two lines with different reactivity to the herbicide, and self-fertilized for 10 generations; the lines were typed by 48 RFLP markers and 66 microsatellites (SSR). Besides seedling tolerance, evaluated as proportion of normal (non-injured) plants after herbicide treatment, other minor components of tolerance were studied: seed germination ability, pollen germination and tube growth in the presence of the herbicide. The analysis, performed by three statistical methods, revealed the presence of factors controlling seedling tolerance on seven chromosomal regions. Five QTLs appeared to be involved in seed germination ability in the presence of Alachlor, four QTLs in pollen tolerance in terms of germination and four in tube growth under stress were detected. Three loci, on chromosomes 1, 7 and 10, explained most of the variation of seedling tolerance, thus being interesting candidate for marker-assisted selection.
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  • 67
    ISSN: 1573-5060
    Keywords: leafy normal-stature ; leafy reduced-stature ; non-leafy normal-stature ; non-leafy reduced-stature ; maize ; short season environments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Development of maize (Zea mays L.) types that produce leaf area rapidly and finish vegetative development quickly would increase production of maize in mid- to short-season areas. The Leafy (Lfy1) and reduced-stature (rd1) traits each make contributions to this end. However, these two traits have not previously been combined. Our objective was to evaluate the morphological aspects of non-leafy normal-stature (NLNS), leafy reduced-stature (LRS), non-leafy reduced-stature (NLRS), and leafy normal-stature (LNS) maize inbreds. Two traits, Lfy1 and rd1, were incorporated into a series of inbreds, resulting in a range of canopy architectures. Twelve variables were recorded for each of 30 inbreds over three years. The 12 variables were: seed emergence, above-ear leaf number, below-ear leaf number, dead leaf number at tasselling, live leaf number at tasselling, total leaf number, above-ear leaf area, ear leaf length, ear leaf width, ear height, internode length, and plant height. Inbreds containing the Lfy1 trait had more above-ear leaf area, above-ear leaf number, dead leaf number at tasselling, total leaf number and number of live leaves at tasselling than non-leafy inbred lines. Below-ear leaf number was not different among LRS, LNS, and NLNS inbred lines. LRS and NLRS inbred lines were also not different for below-ear leaf number. Plant height, ear height, and ear leaf length and width were higher in normal-stature than reduced-stature plants. The proportion of the seeds which emerged was higher for LRS inbreds than the other trait groups.
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  • 68
    ISSN: 1573-5060
    Keywords: leafy normal-stature ; leafy reduced-stature ; non-leafy normal-stature ; non-leafy reduced-stature ; maize ; short season environments ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Development of maize (Zea mays L.) types that produce leaf area and mature quickly would increase production of maize in mid- to short-season areas. The leafy (Lfy1) and reduced-stature (rd1) traits both make contributions to this end. However, these two traits have not previously been combined. Our objective was to evaluate the yield and yield components of non-leafy normal-stature (NLNS), leafy reduced-stature (LRS), non-leafy reduced-stature (NLRS), and leafy normal-stature (LNS) maize inbred lines. The two genes, ‘Lfy1’ and ‘rd1’, were incorporated into a series of inbred lines resulting in a range of canopy architectures. Ten variables were recorded for each of 30 inbred lines over three years. The 10 variables were: corn heat unit requirement from planting to tasselling, corn heat unit requirement from planting to silking, days between tasselling and silking, grain moisture content, husk dry weight, cob dry weight, ear length, maximum ear circumference, grain yield and ratio of grain yield to moisture content. Reduced-stature inbred lines reached anthesis more quickly than normal-stature inbred lines. Grain moisture content was less in reduced-stature inbred lines than normal stature trait groups. Leafy-reduced stature plants had the highest ratio of grain to moisture content and the lowest grain moisture content at harvest. Inbred lines containing the rd1 trait matured more rapidly than other trait groups. The LRS trait group yielded more than the other groups, and showed great potential for use in mid- to short-season environments.
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  • 69
    ISSN: 1573-5060
    Keywords: water stress ; breeding ; adaptation ; G × E ; clustering ; ordination ; Zea mays ; corn ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ten trials evaluated the performance of several late tropical maize populations (La Posta Sequía, Pool 26 Sequía and Tuxpeño Sequía) selected for tolerance to drought during flowering and grain filling and also for yield potential. Families (S1 or full-sib) had been selected recurrently for six to eight years on an index of traits. Pattern (clustering and ordination) analysis was used to analyse the relative performance of entries that included cycles of selection for drought tolerance in the populations and non-drought tolerant checks. Mean environment (E) yields ranged from 1.0 to 10.4 t ha-1. Analysis of variance showed that 97.9% of the total sums of squares was accounted for by E, and that, of the remaining sums of squares the G × E (genotype by environment interaction) was almost 3 times that of the contribution of G alone. Cluster analysis separated the checks, the earlier maturing drought tolerant entries and the later maturing drought tolerant entries. This was verified by principal component (PC) analysis of the G × E matrix. Grouping of the environments (i.e. based on entry performance), resulted in the separation of different types of droughts, and of medium and high yielding well-watered environments. The patterns of discrimination observed indicated that the yield gains under drought would have been unlikely to occur if selection had been done only in well-watered environments. Within each population, selection improved broad adaptation (higher mean yield) to both drought and well-watered environments and cycles of selection ‘jumped’ from non-drought-tolerant to drought-tolerant groups as their specific adaptation to drought environments increased.
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  • 70
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    Plant and soil 188 (1997), S. 319-327 
    ISSN: 1573-5036
    Keywords: agroforestry ; fallow ; maize ; nitrate ; root distribution ; root length density ; root to shoot ratio ; Sesbania sesban
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract One hypothesis for a benefit of integrating trees with crops is that trees with deep root systems can capture and “pump up” nutrients from below the rooting zone of annual crops. Few studies have compared both root and nutrient distribution for planted trees, crops and grassland vegetation. A field study was conducted on a Kandiudalfic Eutrudox in the highlands of western Kenya to measure rooting characteristics and distribution of inorganic N and water in three land-use systems (LUS): (i) Sesbania sesban (L.) Merr. fallow, (ii) uncultivated natural weed fallow and (iii) unfertilized maize (Zea mays L.) monoculture. The maximum rooting depth was 1.2 m in the maize LUS, 2.25 m in a 13-month-old natural fallow, and 〉 4 m in a 15-month-old sesbania fallow. Total root length was 1.26 km m-2 for the maize LUS, 5.98 km m-2 for the natural fallow, and 4.56 km m-2 to 4 m for the sesbania fallow. Root length to 1.2 m was greater (p 〈 0.01) for natural fallow than for maize and sesbania fallow. A considerable portion of the sesbania root length to 4 m was in the subsoil; 47% was at 1.2 to 4 m and 31% was at 2.25 to 4 m. Deep rooting of sesbania coincided with lower soil water below 2 m in the sesbania fallow than the natural fallow. Nitrate-N, but not ammonium-N, to 4 m was affected by LUS. Total nitrate to 4 m was 199 kg N ha-1 for the maize LUS, 42 kg N ha-1 for the natural fallow and 51 kg N ha-1 for the sesbania fallow. Soil nitrate in the maize LUS was highest at 0.3 to 1.5-m depth on this Oxisol with anion sorption capacity. No such accumulation of subsoil nitrate was present under sesbania and natural fallow.
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    Plant and soil 192 (1997), S. 37-48 
    ISSN: 1573-5036
    Keywords: Al toxicity ; Ca deficiency ; maize ; phosphogypsum ; root growth ; subsoil acidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The chemical barrier to root development existing in the subsoils of acid soils is a subject of increasing interest. In order to better understand the factors involved in the amelioration of subsoil acidity, the effects of calcium sulphate, phosphogypsum and calcium carbonate on the properties of the solid and liquid phases of subsoil samples and on the growth and nutrient uptake by maize (Zea mays L.) were evaluated. The soils used were two alic red-yellow latosols, two acric dusky red latosols and one alic dark-red latosol from the State of São Paulo, Brazil. A vertical split-root technique was used in a greenhouse experiment, with the plants initially grown in a small pot with 130 g fertile soil, which was introduced in a larger pot containing 2 dm3 of the subsoil samples. The treatments consisted of a control (C) and applications of calcium carbonate (CC), calcium sulphate (CS) and phosphogypsum (PG) at the rate of 10 mmolc Ca2+ dm-3. CS and PG reduced soil acidity, but in a much smaller proportion than CC. Calcium carbonate reduced the activity of Al3+ because of the increase in pH. Total aluminum and calcium contents in the soil solution were much higher for the red-yellow latosols than for the other soils, indicating lower sorption of Ca2+ and $$SO_4^{2 - } $$ in these soils. The activity of Al in the soil solution was decreased in different ways for the five soils, depending on the ionic strength and the formation of the ionic pair $$AlSO_4^ + $$ and, in the case of PG, the formation of complexes of Al with F (AlF2+, $$AlF_2^ + $$ and $$AlF_3^\bigcirc $$ ). The subsoil samples presented severe restrictions for maize root growth and all three treatments were equally effective in increasing root development, which could be attributed to the supply of calcium in one of the acric dusky red latosols and a combined effect of the amendment in reducing the activity of Al and increasing the activity of Ca in the soil solution in the other soils. As a consequence the three treatments increased in the same manner water, N and K uptake from the subsoil and the dry matter production of maize. It can be concluded that, for the soils considered in this research, phosphogypsum is an effective amendment for acid subsoils containing low calcium or toxic aluminum contents.
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    World journal of microbiology and biotechnology 14 (1997), S. 101-106 
    ISSN: 1573-0972
    Keywords: Batter bread ; fermentation ; Lactobacillus ; maize ; sensory evaluation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Eight lactic acid bacteria were isolated from fermenting maize meal. They were identified as Lactobacillus brevis, L. casei, L. fermentum, Pediococcus acidilacti, P. pentosaceus, Lactobacillus spp. I and Pediococcus spp. I and II. L. brevis and Lactobacillus spp. I isolated from the spontaneously fermented maize meal together with L. brevis isolated from rye sour dough and L. plantarum from ogi, a fermented maize gruel, were selected as starter organisms. There was a decrease in the final pH from 4.9 to 3.8 and an increase in the acid equivalent and temperature of the spontaneously-generated sour maize meal at the end of 24h fermentation. There was a decrease in the pH and moisture content of the sour maize breads relative to the conventional wheat bread. An improvement in the shelf-life of the bread samples was also obtained. Crude protein values of the sour maize breads were between 4.36% and 8.87%, while crude fat contents ranged between 3.66% to 7.67%. The ash contents increased from 2.29% to 2.54% while total carbohydrate values were between 46.31% and 65.3%. Calcium, phosphorus and potassium contents ranged from 0.015, 0.26 and 0.018% to 0.036, 0.47 and 0.036% respectively. Physical examination of the bread samples showed that all were cracked and relatively hard. Weight, height and volume ranged from 316 to 380g; 4.2 to 5.2cm and 200 to 320cm3 respectively. Statistical analysis of the sensory attributes revealed a consumer acceptance of the sour maize breads, although ranking test showed preference for the baker's yeast leavened bread that served as a control.
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    Mitigation and adaptation strategies for global change 1 (1997), S. 251-271 
    ISSN: 1573-1596
    Keywords: Greece ; maize ; climate change ; CO2 effects ; adaptation ; crop simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential impacts of climate change on the phenology and yield of two maize varieties in Greece were studied. Three sites representing the central and northern agricultural regions were selected: Karditsa, Naoussa and Xanthi. The CERES-Maize model, embedded in the Decision Support System for Agrotechnology Transfer (DSSAT 3.0), was used for the crop simulations, with current and possible future management practices. Equilibrium doubled CO2 climate change scenarios were derived from the GISS, GFDL, and UKMO general circulation models (GCMs); a transient scenario was developed from the GISS GCM transient run A. These scenarios predict consistent increases in air temperature, small increases in solar radiation and precipitation changes that vary considerably over the study regions in Greece. Physiological effects of CO2 on crop growth and yield were simulated. Under present management practices, the climate change scenarios generally resulted in decreases in maize yield due to reduced duration of the growing period at all sites. Adaptation analyses showed that mitigation of climate change effects may be achieved through earlier sowing dates and the use of new maize varieties. Varieties with higher kernel-filling rates, currently restricted to the central regions, could be extended to the northern regions of Greece. In the central regions, new maize varieties with longer grain-filling periods might be needed.
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    Mitigation and adaptation strategies for global change 1 (1997), S. 273-288 
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Spain ; wheat ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This study evaluates the theoretical impact of climate change on yields and water use of two crops with different responses to increased CO2 and which represent contrasting agricultural systems in Spain. In all cases the simulated effects of a CO2-induced climate change depended on the counteracting effects between higher daily ET rates, shortening of crop growth duration and changes in precipitation patterns as well as the simulated effects of CO2 on the water use efficiency of the crops. For summer irrigated crops such as maize, the yield reductions and the exacerbated problems of irrigation water availability simulated with climate change may force the crop out of production in some regions. For winter dryland crops such as wheat, productivity increased significantly in some regions, suggesting a northward shift of area suitable for wheat production in future climates. The study considered strategies for improving the efficiency of water use based on the optimization of crop management decisions in a CO2-driven warmer climate.
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  • 75
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    Mitigation and adaptation strategies for global change 1 (1997), S. 273-288 
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Spain ; wheat ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This study evaluates the theoretical impact of climate change on yields and water use of two crops with different responses to increased CO2 and which represent contrasting agricultural systems in Spain. In all cases the simulated effects of a CO2-induced climate change depended on the counteracting effects between higher daily ET rates, shortening of crop growth duration and changes in precipitation patterns as well as the simulated effects of CO2 on the water use efficiency of the crops. For summer irrigated crops such as maize, the yield reductions and the exacerbated problems of irrigation water availability simulated with climate change may force the crop out of production in some regions. For winter dryland crops such as wheat, productivity increased significantly in some regions, suggesting a northward shift of area suitable for wheat production in future climates. The study considered strategies for improving the efficiency of water use based on the optimization of crop management decisions in a CO2-driven warmer climate.
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  • 76
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    Mitigation and adaptation strategies for global change 1 (1997), S. 251-271 
    ISSN: 1573-1596
    Keywords: Greece ; maize ; climate change ; CO2 effects ; adaptation ; crop simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential impacts of climate change on the phenology and yield of two maize varieties in Greece were studied. Three sites representing the central and northern agricultural regions were selected: Karditsa, Naoussa and Xanthi. The CERES-Maize model, embedded in the Decision Support System for Agrotechnology Transfer (DSSAT 3.0), was used for the crop simulations, with current and possible future management practices. Equilibrium doubled CO2 climate change scenarios were derived from the GISS, GFDL, and UKMO general circulation models (GCMs); a transient scenario was developed from the GISS GCM transient run A. These scenarios predict consistent increases in air temperature, small increases in solar radiation and precipitation changes that vary considerably over the study regions in Greece. Physiological effects of CO2 on crop growth and yield were simulated. Under present management practices, the climate change scenarios generally resulted in decreases in maize yield due to reduced duration of the growing period at all sites. Adaptation analyses showed that mitigation of climate change effects may be achieved through earlier sowing dates and the use of new maize varieties. Varieties with higher kernel-filling rates, currently restricted to the central regions, could be extended to the northern regions of Greece. In the central regions, new maize varieties with longer grain-filling periods might be needed.
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    Agroforestry systems 37 (1997), S. 199-207 
    ISSN: 1572-9680
    Keywords: groundnut ; humid lowlands ; labour efficiency ; maize ; partial budgeting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A fallow management trial was initiated in March 1990 in Yaounde, Cameroon, with the objective of identifying an efficient method of managing residue derived from a planted fallow of Cajanus cajan. Ten months after establishment, the shrubs were slashed and residues were treated in one of the following ways: burnt, incorporated in the soil, mulched on soil surface or removed. Following that, maize and groundnut were planted. Soil organic matter was fractionated after the residue treatments, and elementary partial budgeting was conducted. After three cycles of fallow and cropping seasons, maize yield was similar, about 3 t ha-1, in all plots except in the residue-removed plots, where it was 1.4 t ha-1. Yield trend of groundnut was also similar. The residue management method did not affect either the nature of fractions or the total content of soil organic matter. The highest net return, US$5945 ha-1 year-1, was obtained from the residue incorporated treatment, whereas the highest return to labour, US$11 per manday-1 was associated with burning of residues. In areas such as the forest zone of Cameroon where labour is a major constraint and climatic conditions allow vigorous vegetation growth, burning appears to be the best method of residues management, at least in the short run.
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    Plant growth regulation 21 (1997), S. 51-58 
    ISSN: 1573-5087
    Keywords: abscisic acid (ABA) ; ABA metabolism ; leaf water status ; maize ; Commelina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Metabolism and distribution of xylem-fed ABA were investigated in leaves of maize (Zea mays) and Commelina communis when water stress and xylem pH manipulation were applied. 3H-ABA was fed to excised leaves via the transpiration stream. Water stress was applied through either a previous soil-drying before leaves were excised, or a quick dehydration after leaves were fed with ABA. Xylem-delivered ABA was metabolised rapidly in the leaves (half-life 0.7 h and 1.02 h for maize and Commelina respectively), but a previous soil-drying or a post-feeding dehydration significantly extended the half-life of fed ABA in both species. In the first few hours after ABA was fed into the detached leaves, percentages of applied ABA remaining unmodified were always higher in leaves which received water stress treatments than in control leaves. However the percentage decreased to below the control levels several hours later in leaves which received a previous soil-drying treatment prior to excision, but had then been rehydrated by the xylem-feeding process itself. One possible explanation for this could be a changed pattern of compartmentalisation for xylem-carried ABA. A post-feeding dehydration treatment also changed the distribution of xylem-fed ABA within the leaves: more ABA was found in the epidermis of Commelina leaves which had been dehydrated rapidly after ABA had been fed, compared to the controls. The levels of xylem-delivered ABA remaining unmodified increased as the pH of the feeding solution increased from 5 to 8. The results support the hypothesis that water stress and a putative stress-induced xylem pH change may modify stomatal sensitivity to ABA by changing the actual ABA content of the leaf epidermis.
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    Euphytica 98 (1997), S. 141-148 
    ISSN: 1573-5060
    Keywords: maize ; corn ; floury ; open-pollinated ; white ; Zea mays ; landraces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Thirty-five white maize (Zea mays L.) landraces were obtained by the Indian Agricultural Program of Ontario (IAPO) from native farmers in Ontario and New York State between 1986 and 1989. These landraces probably belong to the racial complex Northern Flints and Flours. The 35 landraces, one experimental population and one control hybrid were evaluated in field experiments at two sites in 1993 and 1994. Data were collected on 24 traits in order to characterize and classify the IAPO landraces. Most quantitative traits examined exhibited considerable variation among the IAPO landraces. Variation was also observed within many of the landraces for ear and cob colour, and for endosperm texture. Most ears had eight rows of kernels, but there was some variation for this among and within landraces. Based on seven traits from the two sites in 1994, the IAPO population was grouped into 10 clusters by centroid clustering analysis.
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  • 80
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    Plant and soil 196 (1997), S. 295-300 
    ISSN: 1573-5036
    Keywords: diurnal rhythm ; maize ; nitrate uptake ; root age ; root zones ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The close relationship between nitrate depletion of the subsoil and root-length densities found in field experiments could not be explained by mathematical models simulating nitrate uptake (Wiesler and Horst, 1994). The objective of the present study was the validation of some of the assumptions made in these models namely uniform nitrate-uptake rates (NURs) independent on root age and daytime. Different techniques were developed and compared for the measurement of NUR of different root zones: (i) isolated root segments, (ii) compartmented uptake cuvettes, (iii) depletion of nitrate (water) from agarose blocks placed on specific zones of roots growing in nutrient solution and (iv) in rhizotrones filled with soil over the whole growing cycle of maize plants. All methods yielded a similar magnitude of NUR (10 - 30 pmol cm-2 s-1). However, only intact plants growing in nutrient solution as well as in soil, but not isolated root segments, showed higher NUR at apical root zones compared to more mature branching root zones by a factor of 2 - 8. The NUR of the root apex was particularly sensitive to the nitrogen demand of the plant and the assimilate supply from the shoots as affected by light intensity. At suboptimal, but not at optimal light conditions during preculture, NUR was lower in the dark than in the light. As plants matured, NUR of soil grown plants became increasingly dependent on water uptake. But even if nitrate uptake by mass flow was subtracted from total nitrate uptake, mature roots showed a surprisingly high nitrate-uptake capacity. The results indicate that the formation of root-age classes with different NUR and the assumption of lower NUR at night could improve the modelling of nitrate uptake.
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  • 81
    ISSN: 1573-9058
    Keywords: Al ; carotenoids ; chlorophyll ; fluorescence induction ; Hill reaction ; maize ; singlet oxygen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two weeks old maize (Zea mays L. cv. XL-72.3) plants were submitted to 0 to 81 g m-3 Al for 20 d in a growth medium of low ionic strength. The increasing Al concentrations sharply increased chlorophyll (Chl) concentrations. The rates of photosystem 2 activities (H2O→DCPIP and DPC→DCPIP) increased at 9 g(Al) m-3 but at higher Al doses they decreased again. A slight decrease of qE and qN coupled to an increase of qP was also observed until the 27 g m-3 Al. The Al-induced decline in cytochrome (cyt) b contents per Chl unit was parallel for the b559LP and cyt b559HP forms, but on a leaf area basis more or less opposite trend in both these cyt forms was observed. Increased Al concentrations also decreased carotene and zeaxanthin contents.
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  • 82
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    Photosynthetica 34 (1997), S. 393-400 
    ISSN: 1573-9058
    Keywords: Al, fructose-1,6-bisphosphatase ; intercellular CO2 concentration ; maize ; NADP-malate dehydrogenase ; net photosynthetic rate, photosystems 1 and 2 ; saccharides ; stomatal conductance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two-weeks-old maize (Zea mays L. cv. XL-72.3) plants were submitted to Al concentrations of 0-81 g m-3 for 20 d, after which the A1 concentration-dependent effects on CO2 uptake by the mesophyll tissue and subsequent CO2 assimilation in the photosynthetic carbon reduction cycle of bundle sheath cells were investigated. The net photosynthetic rate (PN) and stomatal conductance (gs) increased continuously up to 27 g m-3 Al, whereas the intercellular CO2 concentration showed minimum values with the 27 g m-3 Al treatment. Moreover, the starch and saccharide concentrations, and fructose-1,6-bisphosphatase did not change significantly with increasing Al concentrations. The photosynthetic electron transport rates along with photosystems 2 and 1 started falling from 9 g m-3 Al onwards, while thylakoid acyl lipid composition did not show a clear pattern. With the Al concentration at 81 g m-3, NADP-malate dehydrogenase activity decreased to minimum values, whereas the opposite occurred with those of pyruvate dikinase, NADP-malic enzyme, and phosphoenolpyruvate carboxylase. Thus in vivo Al concentrations modulate the photosynthetic reduction cycle, possibly by interacting with the carbon flow rate exported to the cytosol. Although the inhibition of NADP-malate dehydrogenase activity might limit pyruvate dikinase, NADP-malic enzyme, and phosphoenolpyruvate carboxylase activities, in vivo the balance between phosphoenolpyruvate production and its carboxylation remains unaffected.
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  • 83
    ISSN: 1573-9058
    Keywords: D1 protein ; internal CO2 concentration ; maize ; oxygen evolution ; phosphoenolpyruvate carboxylase ; photosynthesis ; photosystem 2 ; ribulose-1,5-bisphophate carboxylase/oxygenase ; stomatal conductance ; thermotolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosystem 2 (PS2) in general, and the oxygen-evolving complex (OEC) in particular, is one of the most thermolabile components of photosynthesis. We examined the effects of heat stress on net photosynthetic rate (PN) and content of several stromal and thylakoid-membrane proteins (including OEC proteins) in maize (Zea mays L.) in order to determine if decreases in PN during, and especially after, heat stress were correlated with decreases in the content of OEC proteins. The PN decreased with heat stress in maize, and post-heat stress recovery of PN required 4 d following the second of two heat-shocks. The decrease in PN was not the result of stomatal closure. Cellular levels of the 33, 23, and 16 kDa OEC proteins decreased with heat stress, and the decreases were greatest and most closely correlated with decreases in PN for OEC16. Following the second heat stress, full recovery of OEC levels (especially OEC16 and 33) coincided with full recovery of PN, more so than with other photosynthetic proteins examined. For example, decreases in levels of the 32-kDa QB-binding protein of the PS2 reaction center (D1), ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, and phosphoenolpyruvate carboxylase were generally smaller than for the OEC proteins and full recovery of these proteins occurred at least 2 d prior to full recovery of photosynthesis. These results are consistent with previous fluorescence and in vitro studies by others in suggesting that heat-relaed effects on PS2 and the OEC are an important limitation to Pn during heat stress. Additionally, these results suggest that heat-related decreases in the content of OEC proteins may limit post-heat stress recovery of carbon fixation.
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  • 84
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    Biologia plantarum 40 (1997), S. 109-120 
    ISSN: 1573-8264
    Keywords: chlorohyll ; maize ; 15N-dilution method ; nitrogen fixation ; sodium chloride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pot experiments were conducted to evaluate the possible interaction of salinity (osmotic potential -0.3, -0.9 and -1.2 MPa) and occurrence of Azospirillum lipoferum or exogenous gibberellic acid (GA3) (100 µg g-1) on growth and some physiological parameters of maize. 15N-uptake as well as the percentage of nitrogen derived from 15N-fertilizer were decreased by increasing the NaCl concentrations and completely inhibited at concentrations corresponding to osmotic potentials -0.9 and -1.2 MPa. The percentage of nitrogen originating from N2 fixation was significantly correlated to the total counts of Azospirillum cells that colonized the histosphere. At high NaCl concentrations although no significant changes in N % in shoot dry mass either in inoculated or uninoculated plants were observed, the total N-yield [mg(N) pot-1] was decreased. Fresh and dry shoot mass significantly increased by Azospirillum inoculation. Azospirillum and GA3 treatments were positively correlated with most of the parameters analysed. Azospirillum inoculation or GA3 application at NaCl concentrations up to -1.2 MPa significantly increased the chlorophyll, K, Ca, soluble saccharides and protein contents as compared with control plants.
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  • 85
    ISSN: 1436-5073
    Keywords: Fusarium ; Fusarium toxins ; mycotoxins ; reference materials ; maize ; food contaminants ; feed contaminants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Fumonisins are mycotoxins occurring largely in maize and maize products, which cause animal diseases, such as equine leukoencephalomalacia and porcine pulmonary edema, and may also induce liver cancer on experimental rats. The European Commission Standards, Measurements and Testing (SMT, formerly BCR) Programme, has sponsored a project to improve analytical methodologies for the determination of the two major fumonisins (fumonisin B1 and fumonisin B2) in maize materials. The project involved the following steps: i) the preparation of a blank and a maize material contaminated with fumonisins Bl and B2; ii) a preliminary study of the γ-irradiation conditions for sterilization; iii) homogeneity and stability studies of the maize materials; iv) an intercomparison study for fumonisins analysis in the above materials with the involvement of 24 European laboratories, most of which have national or international responsibilities for foodstuff and/or feedstuff quality control. Results of the intercomparison study are presented together with the homogeneity and stability data relevant to the maize materials.
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  • 86
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    Integrated pest management reviews 1 (1996), S. 251-263 
    ISSN: 1572-9745
    Keywords: storage ; maize ; Prostephanus truncatus ; larger grain borer ; Kenya
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A 4-year project in Kenya to develop management strategies for the larger grain borer (Prostephanus truncatus) in smallholder maize stores is described. The entomological investigations included behavioural and ecological studies of pest activity in experimental and farmers' maize stores and the natural environment, the use of insecticides to protect maize stored as grain and cobs and the release of a biological control agent. The latter was a predatory beetle; this marks the first release of a biological control agent against a storage pest in East Africa. The operation of a national trapping network to assess the extent and future spread of the pest and predator and target control campaigns is outlined. Recommendations for the control of the stored product pests, resulting from these investigations, were tested for likely adoption and modification in the light of socioeconomic surveys and a cost-benefit analysis. A decision tree approach to managing stored product pests is suggested which allows extension workers and farmers to decide the necessity of pesticide application when assessing how best to protect stored maize.
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  • 87
    ISSN: 1573-0832
    Keywords: Aflatoxins ; cyclopiazonic acid ; Penicillium commune ; Aspergillus flavus ; maize ; factorial design
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The combined effects of water activity (aw) and temperature on mycotoxin production by Penicilium commune (cyclopiazonic acid — CPA) and Aspergillus flavus (CPA and aflatoxins — AF) were studied on maize over a 14-day period using a statistical experimental design. Analysis of variance showed a highly significant interaction (P ≤ 0.001) between these factors and mycotoxin production. The minimum aw/temperature for CPA production (2264 ng g−1 P. commune, 709 ng g−1 A. flavus) was 0.90 aw/30 °C while greatest production (7678 ng g−1 P. commune, 1876 ng g−1 A. flavus) was produced at 0.98 aw/20 °C. Least AF (411 ng g−1) was produced at 0.90 aw/20 °C and most (3096 ng g−1) at 0.98 aw/30 °C.
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  • 88
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    Nutrient cycling in agroecosystems 46 (1996), S. 225-234 
    ISSN: 1573-0867
    Keywords: long-term experiment ; maize ; wheat ; fertilizers ; farm yard manure ; weedicide application ; yield sustainability ; zinc deficiency ; nutrient uptake ; cropping sequence ; organic carbon build-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Long-term field experiments play an important role in understanding the complex interactions of plants, soils, climate and management and their effects on sustainable crop production. A long-term fertilizer experiment with maize-wheat-cowpea (fodder) is in progress since 1971 at Punjab Agricultural University farm Ludhiana, India. The experimental result for the first 21 years showed that application of N alone or in combination with P did not produce as much maize and wheat grains as the application of N, P and K together. Eight years after the start of the experiment, the optimal levels of N, P and K application (100% NPK) were unable to sustain the similar (maize) yield level as before because of Zn deficiency. Whereas in FYM amended plots the Zn deficiency did not appear and the higher crop yields could be sustained. The chemical control of weeds could not sustain the maize productivity at the same level as the manual removal of weeds. It was concluded that the high level of crop production can be sustained with the application of N, P and K under intensive cropping system provided deficiency of any of the micronutrient does not crop up. The deficiency of Zn is most likely to occur in semi-arid light textured alluvial soils under intensive cropping without the addition of farm yard manure/organic manures. In maize based cropping systems, manual control of weeds may be preferred to the chemical one. Addition of FYM in conjunction with 100% NPK is most beneficial both from bio-physical and economic point of view.
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  • 89
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    Nutrient cycling in agroecosystems 46 (1996), S. 91-95 
    ISSN: 1573-0867
    Keywords: ammonium thiosulphate (ATS) ; maize ; N uptake ; phenological stages ; N-S fertilisation ; S deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ammonium thiosulphate solution, (ATS, (NH4)2S2O3, 12% NH4-N and 26% S), is a nitrogen-sulphur fertiliser which can also inhibit nitrification, inhibit area hydrolysis and also solubilize micronutrients in alkaline soils. A three year field study was conducted in northeastern Italy to compare the growth, yield, and nutrient uptake of irrigated maize (Zea mays L.) fertilised with 250 kg N ha-1 urea-ammonium nitrate solution (UAN, 30-0-0) or UAN plus ATS. Dry matter (DM) yield, sulphur (S) and nitrogen (N) uptake were measured at several growth stages. Grain was measured and analyzed at maturity. Maize grain yield and N uptake were increased respectively 30.6% and 42.2% in the first year by adding ATS to UAN. Adding 10% by weight ATS to UAN (22.8 kg S ha-1) increased grain yields by 1.9, 1.7 and 1.6t ha-1 for the three years of the study. To distinguish whether the response was due to S or other ATS attributes, ATS was compared to an equivalent amount of S from single superphosphate (SSP). Plots fertilised with ATS gave grain yields 0.5 and 1.2 t ha-1 greater than plots fertilised with equal rates of S from SSP in the last two years of the study. This added yield from ATS over SSP may have been due to beneficial effects of ATS on N or micronutrient availability or to the split application of the S from ATS.
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  • 90
    ISSN: 1573-0867
    Keywords: Agro-ecological zones ; (AEZ) ; cabbages ; economic optimum (fertilizer rates) ; fertilizer response ; GIS ; maize ; modelling ; N ; P ; potatoes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A comprehensive long-term fertilizer trial programme for annual crops was established in 1985 at 70 sites in the high and medium rainfall areas of Kenya testing N and P, farmyard manure and other critical nutrients. Sites were selected according to their representativeness regarding soils and climate in agro-ecological units. The fertilizer trial programme included all major food crops typical for the respective area, both monocropped and intercropped in the case of cereals. Economic optimum fertilizer rates were calculated taking into account input/output price ratios and value-cost ratios. The majority of sites showed responses to N or P, only at one site response to both N and P with a significant interaction was found. Price elasticity of crops such as potatoes and cabbages was higher than that of maize or sorghum. Critical soil P levels were determined for maize at 13 ppm P for modified Olsen extract and 32 ppm P for Mehlich I extract. Variability of rainfall was found to greatly influence viability of fertilizer recommendations. First attempts to extrapolate fertilizer recommendations from areas of representativeness to areas with similar conditions by means of GIS and modelling approaches are discussed.
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  • 91
    ISSN: 1573-0867
    Keywords: cassava ; fertilizer ; intercropping ; maize ; paleustalf
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted on a Paleustalf to examine the effect of fertilizer application and the possible yield limiting factors in a cassava-maize intercropping system. Application of N-fertilizer significantly increased maize grain and cassava root yields as well as N, P and K concentrations of both crops compared with application of non-N fertilizer or with the unfertilized controls. Intercropping, with or without fertilizer application, led to a decrease in the root yield and N, P and K concentrations of cassava, but had no significant effect on nutrient concentrations and grain yield of maize. Growth analysis revealed that from 20 weeks after planting to harvest in cassava and during the flowering to harvest in maize dry matter accumulation in both crops was similar in intercropping. However, during the same period, the mean crop growth rate in cassava was less than a fourth of the mean crop growth rate in maize. It was concluded that the main factor limiting total yield in a cassava-maize intercropping system is the depression of early cassava growth by vigorous maize component, which reduces the amount of assimilate allocated to cassava roots.
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  • 92
    ISSN: 1573-5028
    Keywords: carotenoid biosynthesis ; endosperm ; gene ; maize ; phytoene desaturase ; regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To study regulation of the plastid-localized maize carotenoid biosynthetic pathway, a cDNA encoding phytoene desaturase (PDS) was isolated and characterized. The DNA sequence of the maize Pds cDNA was determined and compared with available dicot Pds genes. The deduced PDS protein, estimated at 64.1 kDa (unprocessed), had a dinucleotide binding domain and conserved regions characteristic of other carotene desaturases. Alignment of available PDS sequences from distantly related organisms suggests that Pds has potential as a phylogenetic tool. By use of heterologous complementation in Escherichia coli, maize PDS was shown to catalyze two desaturation steps converting phytoene to ζ-carotene. RFLP (restriction fragment length polymorphism) mapping was used to place Pds on chromosome 1S near viviparous5 (vp5), and RT-PCR (reverse-transcriptase polymerase chain reaction) analysis indicated reduced Pds transcript in vp5 mutant relative to normal endosperm. Other phytoene-accumulating mutant endosperms, vp2 and white3 (w3), showed no difference in Pds transcript accumulation as compared with normal endosperm counterparts. RT-PCR analysis of Pds transcript accumulation in developing endosperm showed Pds was constitutively expressed. Therefore, endosperm carotenogenesis is not regulated by increasing the level of Pds transcripts.
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  • 93
    ISSN: 1573-5028
    Keywords: maize ; endosperm ; Opaque-2 ; pyruvate orthophosphate dikinase ; phosphoenolpyruvate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pyruvate orthophosphate dikinase (PPDK, E.C. 2.7.9.1) is an abundant enzyme in the leaves of C4 plants associated with the dicarboxylic acid pathways of CO2 fixation in the dark. PPDK activity has also been detected in the seeds of maize and other, non-C4 cereals, where its role has yet to be established. Using an anti-PPDK serum, two cross-reacting species of M r close to 90 000 were detected in developing maize endosperm of wild-type plants. In two independent opaque-2 mutant lines, one of the polypeptides was absent and the other was reduced in level. Similarly, endosperm PPDK mRNA levels were greatly reduced in the opaque-2 maize lines compared to wild type, suggesting that endosperm PPDK gene expression is under Opaque-2 control. However, a low level of PPDK mRNA could still be detected in these mutants, indicating that PPDK gene expression is not absolutely dependent on Opaque-2 but rather can be modulated by it. This interpretation was reinforced by the demonstration that the distribution of PPDK transcripts is not affected in o2 mutants, although the level is reduced, and that PPDK mRNA is detectable prior to 02 mRNA during the maturation of wild-type maize endosperm. Using oligonucleotides specific for the different maize PPDK genes, the o2 mutations were shown to affect only cyPPDKZml gene expression in maize line A69Y.
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  • 94
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    Plant molecular biology 32 (1996), S. 1159-1161 
    ISSN: 1573-5028
    Keywords: opaque-2 ; Bg ; transposon ; endosperm ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The molecular defect in an opaque-2 (o2) mutant, previously characterized as a null allele, has been identified as containing an insertion of the transposable element of the Bergamo (Bg) family. Restriction mapping and partial sequence analysis of the Bg in the o2 null allele indicates that this element is distinct from the previously described Bg as well as the defective Bg (rbg) of the o2m(r) allele. It is, however, inserted at the same site in O2 as the rbg of 62m(r) and can transpose when Bg is present. This study shows that, depending on genetic background, this allele may not behave as a stable null which could dramatically influence the conclusions drawn from experiments based on this particular mutant.
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  • 95
    ISSN: 1573-5028
    Keywords: Caffeic acid O-methyltransferase ; in situ hybridization ; lignification ; maize ; maize transformation ; tobacco transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pattern of expression directed by the promoter of the maize caffeic acid O-methyltransferase (COMT) gene was studied by histochemical and fluorometric β-glucuronidase (GUS) analysis in transgenic maize and tobacco plants. The COMT promoter directs GUS expression to the xylem and the other tissues undergoing lignification, and it responds to wounding and to elicitors. In transgenic maize plants, expression of GUS corresponds to the pattern of expression of the endogenous COMT gene as determined by northern analysis and in situ hybridization. The pattern in transgenic tobacco plants clearly shows that the maize promoter sequence is recognized by tobacco transcriptional factors, in spite of the anatomical differences and the evolutionary distance between these two species. The results suggest that the most significant promoter signals that induce the specific expression of the lignin COMT are conserved in different species.
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  • 96
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    Transgenic research 5 (1996), S. 219-221 
    ISSN: 1573-9368
    Keywords: maize ; immature embryo ; nptII ; gus ; electroporation ; transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using the pulse-discharging electroporation system HPES-3, we have transferred the neomycin phosphotransferase II (nptII) gene and β-glucuronidase (gus) gene into mechanically-woulded immature zygotic embryo cells of an elite local maize cultivar Huanong Supersweet No. 42 and have produced transgenic maize plants. DNA hybridization and NPTII dot assay showed that the foreign genes were integrated into the genomes and expressed stably in the cells of the transgenic calluses and plants.
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  • 97
    ISSN: 1573-5036
    Keywords: lettuce ; maize ; phosphorus solubilization ; plant growth promoting rhizobacteria (PGPR) ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Rhizobium leguminosarum bv. phaseoli strains P31 and R1, Serratia sp. strain 22b, Pseudomonas sp. strain 24 and Rhizopus sp. strain 68 were examined for their plant growth-promoting potential on lettuce and forage maize. All these phosphate solubilizing microorganisms (PSM) were isolated from Québec soils. The plants were grown in field conditions in three sites having high to low amounts of available P. In site 1 (very fertile soil), strains R1 and 22b tended to increase the dry matter yield of lettuce shoots (p≤0.10). Lettuce inoculated with rhizobia R1 had a 6% higher P concentration (p≤0.10) than the uninoculated control. In site 2 (poorly fertile soil), the dry matter of lettuce shoots was significantly increased (p≤0.05) by inoculation with strain P31 and 24 plus 35 kg ha-1 P-superphosphate, or with strain 68 plus 70 kg ha-1 P-superphosphate. In site 3 (moderately fertile soil), the dry matter of maize shoots was significantly increased (p≤0.05) by inoculation with strain 24 plus 17.5 kg ha-1 P-superphosphate, or with strain P31 plus 35 kg ha-1 P-superphosphate. Inoculation with PSM did not affect lettuce P uptake in the less fertile soil in site 2. In site 3 with the moderately fertile soil, maize plants inoculated with strain R1 had 8% higher P concentration than the uninoculated control (p≤0.01), and 6% with strains P31 and 68 (p≤0.05). The results clearly demonstrate that rhizobia specifically selected for P solubilization function as plant growth promoting rhizobacteria with the nonlegumes lettuce and maize. The P solubilization effect seems to be the most important mechanism of plant growth promotion in moderately fertile and very fertile soils when P uptake was increased with rhizobia and other PSM.
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  • 98
    ISSN: 1573-5036
    Keywords: maize ; root growth model ; root mapping ; root spatial distribution ; root system ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Most existing water and nutrient uptake models are based on the assumption that roots are evenly distributed in the soil volume. This assumption is not realistic for field conditions, and significantly alters water or nutrient uptake calculations. Therefore, development of models of root system growth that account for the spatial distribution of roots is necessary. The objective of this work was to test a three dimensional architectural model of the maize root system by comparing simulated horizontal root maps with observed root maps obtained from the field. The model was built using the current knowledge on maize root system morphogenesis and parameters obtained under field conditions. Simulated root maps (0.45 × 0.75 m) of horizontal cross sections at 3 depths and 3 dates were obtained by using the model for a plant population. Actual root maps were obtained in a deep, barrier-free clay-loamy soil by digging pits, preparing selected horizontal planes and recording root contacts on plastic sheets. Results showed that both the number of cross-sections of axile roots, and their spatial distribution characterized with the R-index value of Clark and Evans (1954), were correctly accounted for by the model at all dates and depths. The number of cross-sections of laterals was also correctly predicted. However, laterals were more clustered around axile roots on simulated root maps than on observed root maps. Although slight discrepancies appeared between simulated and observed root maps in this respect, it was concluded that the model correctly accounted for the general colonization pattern of the soil volume by roots under a maize crop.
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  • 99
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    Plant and soil 186 (1996), S. 29-38 
    ISSN: 1573-5036
    Keywords: Acetobacter ; Azoarcus ; Hebaspirillum ; Klebsiella ; maize ; sugar cane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The development of nitrogen fixation in maize can be considered the “holy grail” of nitrogen fixation research. As nitrogen fertilization is one of the highest costs of corn production, the development of a symbiosis between diazotrophic bacteria and corn would be of enormous economic value. Such a discovery would also improve human health as it would decrease the amount of nitrate in ground water as well as in corn cultured for human consumption. Several proposals have been made toward this end. These include: a) the transfer of root nodulation genes from a legume to maize; b) the expression of the bacterial nif regulon in maize organelles; and c) the development of corn lines with the ability to accept fixed nitrogen from diazotrophs in the rhizosphere. All of these proposals have enormous technical problems to overcome such that the development of nitrogen-fixing corn in the near term has been considered unlikely. An alternative and less-technically challenging approach may be a thorough study of non-pathogenic bacterial endophytes that already inhabit the corn plant. The discovery of a nitrogen-fixing bacterial-sugar cane association by Döbereiner and coworkers in Brazil illustrates the enormous potential of endophytic bacteria to enhance grass biomass in the absence of nitrogen fertilizer. Döbereiner and coworkers have discovered diazotrophic strains of Acetobacter diazotrophicus and Herbaspirillum seropedicae in lines of sugar cane that were bred in the absence of nitrogen fertilizer. The Brazilian group has also demonstrated that sugar cane plants infected with these diazotrophs are capable of deriving all of their nitrogen needs from N2. Recently, the presence of non-pathogenic endophytic bacteria in corn has been shown. Based on this evidence and using the sugar cane paradigm as an example, investigators are working toward the discovery and analysis of diazotrophic endophytes in corn which includes the search for corn germplasm that would readily benefit from an association with these bacteria. Several diazotrophic endophytes have been identified in grass species that are members of the α-, β-, and γ-subclasses of the proteobacteria. Our understanding of the ability of these bacteria to enhance the growth of grasses through nitrogen fixation is only beginning to be explored but this approach is thought to be far less technically challenging than are other proposals to develop nitrogen fixation in maize.
    Type of Medium: Electronic Resource
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  • 100
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 89 (1996), S. 291-296 
    ISSN: 1573-5060
    Keywords: maize ; Zea mays ; phenotypic correlation ; genotype × environment interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study was conducted to determine the relationships among various agronomic traits in inbreds, hybrids and between inbreds and their progeny of maize in short season areas. Phenotypic correlations of each measured trait between as well as among hybrids and inbreds were not the same. The highest correlation coefficient (r=0.78) was obtained between days to anthesis and yield. The high yielding inbreds did not necessarily give rise to high yielding progeny. Given that days to anthesis plays an important role in two major traits (yield and moisture) for hybrids, an emphasis on optimum anthesis dates during inbred development could be a useful criterion for the prediction of hybrid performance in short season areas.
    Type of Medium: Electronic Resource
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