ALBERT

Notizen: Abstract Three experiments were undertaken to investigate the influence of seed mixture on the establishment of a target grassland community on a site with high available phosphorus. In the first experiment autumn- and spring-sown commercial seed mixtures were compared with seed harvested from a nature reserve with respect to their ability to produce an inundation grassland community similar to that described by the British National Vegetation Classification (NVC) as Agrostis stolonifera–Alopecurus geniculatus grassland (MG13). In the second experiment the composition and sowing rate of a commercial seed mixture were altered to investigate whether these factors were significant in the establishment of a sward similar to MG13. Similarly, in the third experiment the composition of a commercial seed mixture designed to achieve an alternative community, Cynosurus cristatus–Caltha palustris grassland (NVC code MG8), was sown. The vegetation resulting from each of these treatments was monitored with permanent quadrats for a 3-year period, and the hydrological regime of each quadrat location was modeled and quantified. The results showed that seed mixture, timing of sowing, and seeding rate had an initial effect on the vegetation that established. However, by the third year of monitoring there were no significant differences between these treatments, and hydrological regime had become the most important factor in determining the distribution of species. The vegetation was less diverse than predicted from germination tests and decreased in diversity over the monitoring period. It is suggested that this may be a result of the hydrological regime being unsuitable for several of the sown species or due to the extremely high available phosphorus concentration in the soil. This study highlights the need to understand the soil and hydrological conditions of a site before choosing a target community and designing a seed mixture.

Notizen: Abstract Introduced perennial grasses are one of the greatest constraints to prairie restoration. Herbicides suppress but do not eliminate introduced grasses, so we explored the interaction of herbicide with two additional controls: heavy clipping (to simulate grazing) and competition from native species. A 50-year-old stand of the introduced perennial grass Agropyron cristatum (crested wheatgrass) in the northern Great Plains was seeded with native grasses and treated with herbicide annually for 7 years in a factorial experiment. Clipping was applied as a subplot treatment in the final 3 years. Both herbicide and clipping significantly reduced the cover of A. cristatum, but clipping produced an immediate and consistent decrease, whereas herbicide control varied among years. The cover of A. cristatum decreased significantly with increasing cover of a seeded native grass, Bouteloua gracilis (blue grama), suggesting that both top-down (i.e., grazing) and bottom-up (i.e., resource competition) strategies can contribute to A. cristatum control. No treatment had any effect on the seed bank of A. cristatum. Even in the most effective control treatments, A. cristatum persisted at low amounts (approximately 5% cover) throughout the experiment. The cover of B. gracilis increased significantly with seed addition and herbicide, and, after 7 years, was similar to that in undisturbed prairie. The total cover of native species increased significantly with clipping and herbicide, and species richness was significantly higher in plots receiving herbicide. Clipping season had no effect on any variable. In summary, no method extirpated A. cristatum, but clipping reduced its cover by 90% and doubled the cover of native species. Extirpation might not be a realistic goal, but relatively simple management allowed coexistence of native species.

Notizen: Abstract The reduction and fragmentation of forest habitats is expected to have profound effects on plant species diversity as a consequence of the decreased area and increased isolation of the remnant patches. To stop the ongoing process of forest fragmentation, much attention has been given recently to the restoration of forest habitat. The present study investigates restoration possibilities of recently established patches with respect to their geographical isolation. Because seed dispersal events over 100 m are considered to be of long distance, a threshold value of 100 m between recent and old woodland was chosen to define isolation. Total species richness, individual patch species richness, frequency distributions in species occurrences, and patch occupancy patterns of individual species were significantly different among isolated and nonisolated stands. In the short term no high species richness is to be expected in isolated stands. Establishing new forests adjacent to existing woodland ensures higher survival probabilities of existing populations. In the long term, however, the importance of long-distance seed dispersal should not be underestimated because most species showed occasional long-distance seed dispersal. A clear distinction should be made between populations colonizing adjacent patches and patches isolated from old woodland. The colonization of isolated stands may have important effects on the dynamics and diversity of forest networks, and more attention should be directed toward the genetic traits and viability of founding populations in isolated stands.

Notizen: Abstract The effects of the earthworm Pontoscolex corethrurus (Muller) on the rate of mineralization of cattle dung-amended iron (Fe2+) ore mine wastes and its preference for partially decomposed leaf litter with contrasting chemical composition were studied in pot trials. The growth and survival rates of earthworms showed significant positive correlations with percent of organic matter. During 96 days of exposure, the earthworms significantly increased exchangeable Ca2+, Mg2+, PO43− and NH4-N. Iron ore mine wastes amended with 5–10% organic matter supported earthworm fauna better than mine wastes amended with 0–3% organic matter. The leaf litter preference shown by the earthworm was, in descending order, Phyllanthus reticulatus, Tamarindus indica, Anacardium occidentale, Casuarina equisetifolia, Acacia auriculiformis, and Eucalyptus camaldulensis. A significant positive correlation was observed between the survival and growth rates of earthworms and the nutrient contents of partially decomposed leaf litter. The first three plant species were significantly richer in nutrients, mainly organic carbon, calcium, phosphorus, and nitrogen, than the other two plant species. Acacia auriculiformis and E. camaldulensis litter were preferred less because of their high lignin and polyphenolic compounds, despite being rich in other macronutrients like nitrogen and phosphorus. It is concluded that the introduction of P. corethrurus to cattle dung-amended (5–10%) iron ore mine wastes or revegetation of the sites with P. reticulatus, T. indica, and A. occidentale plant species should be attempted before earthworm introduction. The litter from these species acts as a source of food for earthworms, thereby hastening the process of restoration of abandoned iron ore mines of Goa, India.

Notizen: Abstract River and stream rehabilitation projects are increasing in number, but the success or failure of these projects has rarely been evaluated, and the extent to which buffers can restore riparian and stream function and species composition is not well understood. In New Zealand the widespread conversion of forest to agricultural land has caused degradation of streams and riparian ecosystems. We assessed nine riparian buffer zone schemes in North Island, New Zealand that had been fenced and planted (age range from 2 to 24 years) and compared them with unbuffered control reaches upstream or nearby. Macroinvertebrate community composition was our prime indicator of water and habitat quality and ecological functioning, but we also assessed a range of physical and water quality variables within the stream and in the riparian zone. Generally, streams within buffer zones showed rapid improvements in visual water clarity and channel stability, but nutrient and fecal contamination responses were variable. Significant changes in macroinvertebrate communities toward “clean water” or native forest communities did not occur at most of the study sites. Improvement in invertebrate communities appeared to be most strongly linked to decreases in water temperature, suggesting that restoration of in-stream communities would only be achieved after canopy closure, with long buffer lengths, and protection of headwater tributaries. Expectations of riparian restoration efforts should be tempered by (1) time scales and (2) spatial arrangement of planted reaches, either within a catchment or with consideration of their proximity to source areas of recolonists.

Notizen: Abstract One of the greatest challenges for ecologists this century will be restoring forests on degraded tropical lands. This restoration will require understanding complex processes that shape successional pathways, including interactions between trees and other plants. Shrub species often quickly invade disturbed tropical lands, yet little is known about whether they facilitate or inhibit subsequent tree recruitment and growth. We examined how shrubs and other vegetation (e.g., vines, grasses, herbs) affect tree recruitment, survival, and growth during the first 6 years of forest succession in Kibale National Park, Uganda. The study was undertaken in two recently logged exotic softwood plantations. We studied the successional trajectories in two recently logged areas that varied in their initial densities of trees and shrubs. Analyses suggested tree seedling presence and density were not strongly related to shrub density or height during succession. Tree sapling presence and density were positively significantly related to shrub density and height. We found little response in the tree community to experimental shrub removal, and although removal of all nontree vegetation temporarily enhanced tree growth, the effect disappeared after 2 years. Some early-successional trees benefited from reduced competition, whereas some mid-successional trees benefited from the presence of other vegetation. Some specific tree species responded strongly to vegetation removal. We interpret our findings in light of designing manipulations promoting forest restoration for biodiversity conservation and conclude with four tentative guidelines: (1) manage at the species level, not the community level; (2) increase facilitation for seedlings, reduce competition for saplings; (3) be cautious of assumptions about plant interactions; and (4) be adaptable and creative with new strategies when manipulations fail.

Notizen: Abstract Soil chemical properties and soil mesofauna composition were assessed at a forest site in northern Austria, where 20 years earlier an amelioration treatment had been performed. The site had been treated with limestone, a high P slag, and ammonium nitrate to replace the poorly growing pine (Pinus sylvestris) forest with a Norway spruce (Picea abies) stand. This treatment was at that time a common means for the amelioration of nutrient-poor forest soils with recalcitrant forest floor layers. After treatment, a dense cover of a nitrophilic stinging nettle (Urtica dioica) developed. Most likely, the site had been over-fertilized, and inadvertently, an experiment with extreme N enrichment had been conducted. The abundance of collembolans increased, and dominance structure shifted from Isotomiella minor, Lipothrix lubbocki, and Isotoma notabilis at fertilizer treatment to Friesea mirabilis, Isotomiella minor, and Sphaeridia pumilis in the control, but the abundance of soil mesofauna generally decreased in the fertilizer treatment. Fertilization reduced the mass of the litter layer from 7.6 to 2.4 kg/m2. The total carbon pool in the soil was reduced due to reduction of the litter layer. However, the content of soil organic matter in the upper mineral soil was significantly increased. A part of the applied and mineralized nitrogen had been lost from the soil, but N retention in the upper mineral soil was still considerable. Soil pH and the base saturation were sustainably increased. Carbon losses upon mineralization of the litter layer were not offset by the increase in C content of the mineral soil. Presently, the C pool in the soil of the fertilized treatment is lower than in the control. However, the overall nutrient enrichment of the soil may facilitate C sequestration in the fertilized site in the future.

Notizen: Abstract Poor growth of Quercus robur L. (oak) trees has been reported on mine sites where overburden and subsoil have been used in the reinstatement of surface layers. This stunting has been attributed to a lack of macronutrients and to an adverse soil environment for root growth and mycorrhizal development. Growth, mineral nutrition, and ectomycorrhizal colonization of Q. robur seedlings were studied in an experiment carried out under controlled growing conditions in which mine spoil material was enriched with a leaf litter mulch. Enrichment of mine spoil material was found to produce a significant increase in growth and foliar N concentrations of oak seedlings. Inoculation with three taxa of ectomycorrhizal fungi did not benefit seedlings when mine spoil was the only substrate, possibly due to the poor physical properties of the unamended spoil and lack of nutrients. Inoculation with two taxa, Laccaria laccata and Hebeloma crustuliniforme, isolated from 3-year-old trees produced a significant stimulation of growth in the organically enriched treatment, which was believed to be due to greater uptake of mineralized N. However, Cortinarius anomalus isolated from fruit bodies associated with a 15-year-old tree did not increase biomass. The presence of organic matter was found to result in a significant stimulation of mycorrhizal infection by both inoculum and contaminant mycobionts. Recommendations are made for improving the establishment and growth of oak seedlings on reinstated sites.

Notizen: Abstract Prairie restoration at the northern edge of the Great Plains can be frustrated by previously established non-native perennial grasses. We compared the emergence of a widely introduced grass, Agropyron cristatum, and a common native grass, Bouteloua gracilis, in a 4-year-old field experiment in which the Agropyron-dominated vegetation had either been left intact or treated annually with herbicide. This was done at two levels of water supply, reflecting conditions expected in wet and dry years, to examine the effects of among-year variability in precipitation. Water addition significantly increased the emergence of both surface-sown and buried (1 cm deep) seeds. Herbicide treatment of neighbors did not increase the emergence of experimentally added seeds. Emergence was much greater for buried (80%) than surface-sown seeds (20%). Significantly more Bouteloua than Agropyron germinated from experimentally buried seeds. Whereas only a single seedling of Bouteloua emerged from the existing seed bank, the mean density of Agropyron seedlings emerging from the seed bank was 930/m2 (range, 0 to 6,455/m2). Surprisingly, the emergence of Agropyron from the seed bank was not decreased by 4 years of herbicide treatment, possibly because herbicide may release Agropyron from intraspecific competition and allow increased seed production to compensate for decreased plant abundance. In summary, we found few differences between Agropyron and Bouteloua in spring and summer emergence at high or low water availability. The persistence of Agropyron stands despite repeated herbicide application may be partly due to increased seed production.

Notizen: Abstract The effect of increasing planting unit size and stabilizing sediment was examined for two seagrass planting methods at Carnac Island, Western Australia in 1993. The staple method (sprigs) was used to transplant Amphibolis griffithii (J. M. Black) den Hartog and the plug method was used to transplant A. griffithii and Posidonia sinuosa Cambridge and Kuo. Transplant size was varied by increasing the number of rhizomes incorporated into a staple and increasing the diameter of plugs. Planting units were transplanted into bare sand, back into the original donor seagrass bed, or into a meadow of Heterozostera tasmanica, which is an important colonizing species. Sprigs of A. griffithii were extracted from a monospecific meadow; tied into bundles of 1, 2, 5, and 10 rhizomes; and planted into unvegetated areas. Half the units were surrounded by plastic mesh and the remainder were unmeshed. All treatments were lost within 99 days after transplanting, and although larger bundles survived better than smaller ones, no significant differences could be attributed to the effects of mesh or sprig size. Plugs of P. sinuosa and A. griffithii were extracted from monospecific meadows using polyvinyl chloride pipe of three diameters, 5, 10, and 15 cm, and planted into unvegetated areas nearby. Half the units were surrounded by plastic mesh and the remainder were unmeshed. Posidonia sinuosa plugs were also placed within a meadow of H. tasmanica (Martens ex Aschers.) den Hartog. Only 60% of A. griffithii plug sizes survived 350 days after transplanting back into the donor bed; however, survival of transplants at unvegetated areas varied considerably, and analysis of variance indicated a significant two-way interaction between treatment and plug size. Transplants survived better when meshed (90% survived) and survival improved with increasing plug size. Posidonia sinuosa transplants survived poorly (no plugs survived beyond 220 days in bare or meshed treatments) regardless of size. Survival of 10- and 15-cm plugs was markedly better than the 5-cm plugs in vegetated areas, including the H. tasmanica meadow. The use of large seagrass plugs may be appropriate for transplantation in high-energy wave environments.

Notizen: Abstract Dominance-diversity curves have been previously constructed for a range of ecosystems around the world to illustrate the dominance of particular species and show how their relative abundances compare between communities separated in time or space. We investigate the usefulness of dominance-diversity curves in rehabilitated areas to compare the floristic composition and abundance of “undisturbed” areas with disturbed areas, using bauxite mining rehabilitation in Western Australia as an example. Rehabilitated pits (11–13 years old) subjected to prescribed fire in autumn and spring were compared with unburned rehabilitated areas and the native jarrah (Eucalyptus marginata) forest. Dominance diversity curves were constructed by ranking the log of the species density values from highest to lowest. Species were categorized according to a variety of functional responses: life form (trees, shrubs, subshrubs, and annuals), fire response syndrome (seeder or resprouter), nitrogen fixing capability, and origin (native or adventive). Exponential functions showed extremely good fits for all sites (r2 = 0.939–0.995). Dominance diversity graphs showed that after burning of rehabilitated areas, sites exhibited a more similar dominance-diversity curve than before burning. This was emphasized in a classification (UPGMA) of the regression equations from the dominance-diversity curves that showed that sites burned in spring were more similar to the native forest than sites burned in autumn. There was no significant segregation of the nitrogen-fixing and species origin categories, although the life form and fire response groupings showed significant segregation along the dominance-diversity curve. Resprouters tended to be over-represented in the lower quartiles and under-represented in the upper quartiles of post-burn sites. It is suggested that using dominance-diversity curves in the monitoring of rehabilitated areas may be a useful approach because it provides an easily interpretable visual representation of both species richness and abundance relationships and may be further utilized to emphasize categories of plants that are over- or under-represented in rehabilitated areas. This will assist in the post-rehabilitation management of these sites.

Notizen: Abstract The wave-exposed nature of much of the southwestern Australian coastline considerably reduces the protective influence of seagrasses, and sediment movement appears to be relatively unaffected by their presence. Present seagrass restoration efforts focus on the deployment of large mechanically transplanted “sods” of seagrass as a means of combating the negative effects of water motion on transplant survival. The aim of this study was to investigate the combined role of wave energy and transplant spacing on sediment movement and transplant survival to provide guidance for seagrass transplantation in areas of high wave energy. One hundred sixty sods (0.25 m2) of seagrass were mechanically extracted from a mixed meadow consisting of Amphibolis griffithii (Cymodoceaceae) and Posidonia coriacea (Posidoniaceae) and planted in a high wave energy site with the treatments configured as three replicates of 16 sods placed in 4 × 4–meter squares at distances of 0.5, 1.0, and 2.0 meters apart. An additional 16 single sods were planted randomly throughout the site. Monitoring was conducted at two monthly intervals and consisted of counting the number of sods surviving and measuring the shoot density of seagrass species within each surviving sod. Sediment height was monitored using a series of sediment plates and an electronic sediment level sensor. Sod spacing had no significant effect upon transplant survival, which remained above 90% for 4 months after transplantation and then declined with the onset of winter (June to August). After 14 months individual sod survival was between 9% and 40%. Initial shoot densities were 200 to 500 shoots/m2 and declined to less than 50 shoots/m2. Sediment fluctuations up to 35 cm were noted, occasionally taking place over a matter of hours, and storms during winter caused significantly increased sediment movement. This probably curtailed rhizome extension and prevented the expansion of the transplants. This study indicates that the ability of seagrasses to influence sediment would appear to vary with the prevailing hydrodynamic regime and that a reappraisal of the notion that all seagrass communities trap sediment is necessary.

Notizen: Abstract Logging, fire suppression, and urbanization have all contributed to the serious decline and fragmentation of Pinus palustris (longleaf pine) ecosystems in the southeastern United States. Effective management of the remaining patches of these pyrogenic communities must incorporate periodic low-intensity fires, even where they are located on private lands in populated urban and suburban areas. To explore the effects of fire and its potential use for restoration and management of small fragments surrounded by suburban development, we conducted growing season prescribed fires in remnant longleaf pine sandhill patches in the suburbs of Gainesville, Florida. Density and composition of hardwoods were surveyed pre-burn and 1 and 9 months post-burn. Woody stem density decreased in the burn plots, predominantly in the smaller size classes. Flowering responses of forbs and small shrubs were surveyed six times post-burn for 1 year. Overall, the burns did not yield greater densities of flowering stems, but burn patches had higher species richness and diversity than control patches. In addition, there were consistently greater numbers of “showy flowered” sandhill species in flower in burn patches relative to controls. The results of this research demonstrate that prescribed fire can be used for restoration and management of small remnants of longleaf pine sandhill in suburban neighborhoods. It is also clear that although a single prescribed burn can be effective, it will take more than one burn to attain desired restoration goals in degraded longleaf remnants.

Notizen: Abstract Efficient and accurate vegetation sampling techniques are essential for the assessment of wetland restoration success. Remotely acquired data, used extensively in many locations, have not been widely used to monitor restored wetlands. We compared three different vegetation sampling techniques to determine the accuracy associated with each method when used to determine species composition and cover in restored Pacific coast wetlands dominated by Salicornia virginica (perennial pickleweed). Two ground-based techniques, using quadrat and line intercept sampling, and a remote sensing technique, using low altitude, high resolution, color and color infrared photographs, were applied to estimate cover in three small restoration sites. The remote technique provided an accurate and efficient means of sampling vegetation cover, but individual species could not be identified, precluding estimates of species density and distribution. Aerial photography was determined to be an effective tool for vegetation monitoring of simple (i.e., single-species) habitat types or when species identities are not important (e.g., when vegetation is developing on a new restoration site). The efficiency associated with these vegetation sampling techniques was dependent on the scale of the assessment, with aerial photography more efficient than ground-based sampling methods for assessing large areas. However, the inability of aerial photography to identify individual species, especially mixed-species stands common in southern California salt marshes, limits its usefulness for monitoring restoration success. A combination of aerial photography and ground-based methods may be the most effective means of monitoring the success of large wetland restoration projects.

Notizen: Abstract Possible constraints on the passive recovery of bare areas in the Karoo, a semiarid region in South Africa, include inadequate supply of seed, availability of suitable microsites for plant establishment, altered soil properties, and the truncation of key soil biotic processes. Here we investigate the possibility of initiating the restoration of bare areas by soil surface treatments with gypsum (CaSO4) and/or organic mulch. We also apply an exogenous seed source to test the hypothesis that seed availability limits autogenic recovery. Both gypsum and mulch improved rain water infiltration, gypsum more so than mulch, and both treatments resulted in significantly higher numbers of reseeded seedlings compared with controls. Gypsum also improved the survival of the cohorts of seedlings of the larger seeded Tripteris sinuata. Tripteris showed the highest number of seedlings (maximum count of 150 seedlings/1,000 viable seeds sown) and surviving plants of the three reseeded species, which included two small-seeded species, Ruschia spinosa and Chaetobromus dregeanus. Throughout the study period significantly higher plant volumes of naturally seeded annuals and perennials were recorded in the gypsum and/or mulch treatments compared with the controls. Germination and emergence of reseeded and naturally seeded plants appears to be determined by the availability of cool season (autumn to spring ) soil moisture, whereas follow-up rainfall during this time is important for plant survival. Mulching of bare areas in the Succulent Karoo has the potential to re-create vegetated areas that will further capture and conserve water, soil, and nutrients. Gypsum also showed positive results but might not be a cost-effective option because of transport costs to these remote arid areas.

Notizen: Abstract Spring burning of sedge-grass meadows in the Slave River Lowlands (SRL), Northwest Territories, Canada was applied between 1992 and 1998 to reduce shrub encroachment and enhance Bison bison (bison) habitat, although the impact of fire on preferred bison forage was unknown before management. In the summer of 1998 we conducted a study in the Hook Lake area of the SRL to test the effect of burn frequency (unburned, burned once, or burned three times since 1992) on herbaceous plant community composition and Salix spp. L. (willow) shrub vigor. Plant species abundance, litter biomass, soil pH, and depth of the organic soil horizon were measured in 300 1-m2 quadrats nested within 30 1,000-m2 plots in both burned and unburned dry meadows. To test the relationship between frequency and willow vigor, all willow shrubs within the plots were assigned a vigor score from I (dead) to IV (flourishing). The spring burns appear to have reduced willow vigor; however, shrub survival remained high (76%) on the most frequently burned meadows. Ordination plots resulting from canonical correspondence analysis suggest that multiple spring burns influenced plant community composition in dry meadow areas and that less palatable bison forage species (e.g., Carex aenea Fern. and Juncus balticus L.) were correlated with a regime of three spring burns. Our results suggest that frequent spring fires in the Hook Lake area have only a small negative effect on willow cover but may reduce the abundance of primary bison forage plants compared with less frequently burned meadows.

Notizen: Abstract Although there is no one correct technique for sampling vegetation, the sampling design chosen may greatly influence the conclusions researchers can draw from restoration treatments. Considerations when designing vegetation sampling protocol include determining what sampling attributes to measure, the size and shape of the sampling plot, the number of replicates and their location within the study area, and the frequency of sampling. We installed 20 point-intercept transects (50-m long), 8 belt transects (10 × 50 m), 10 adapted Daubenmire transects (four 0.5 × 2-m plots), and 4 modified-Whittaker plots (20 × 50 m with smaller nested plots) in treatment and control units to measure understory herbaceous response in a forest restoration experiment that tested different treatments. Point-intercept transects on average recorded at least twice as much plant cover as did adapted Daubenmire transects and modified-Whittaker plots taken at the same location for all control and treatment units. Point-intercept transects and adapted Daubenmire plots on average captured fewer rare and exotic species in the control and treatment units in comparison with the belt transects and modified-Whittaker plots. Modified-Whittaker plots captured the highest species richness in all units. Early successional understory response to restoration treatments was likely masked by the response of the herbaceous community to yearly climatic variation (dry vs. wet years). Species richness and abundance were higher in wet years than dry years for all control and treatment units. Our results illustrate that sampling techniques can greatly influence perceptions of understory plant trajectories and therefore the interpretation of whether restoration goals have been achieved. In addition, our results suggest that restoration monitoring needs to be conducted for a sufficient length of time so that restoration treatment responses can be detected.

Notizen: Abstract A comparison of a composted organic amendment, a controlled-release fertilizer, and induced mycorrhizal inoculation as affecting the establishment and nutrition of bareroot Jeffrey pine (Pinus jeffreyi Grev. & Balf.) was conducted on a Sierra Nevada surface mine. The soil amendments were applied at outplanting to the backfill of augered planting holes, with a low rate of 8 g and a high rate of 16 g per seedling for the fertilizer, Gromax 21-6-2 + Minors, whereas a single rate of 2.0 L was used for organic matter. Colonization by Pisolithus tinctorius (Pers.) Coker & Couch was induced by coating the root systems with basidiospores suspended in a gel carrier. The organic amendment especially, but also mycorrhizal inoculation, caused substantial seedling mortality, whereas survival was unaffected by controlled-release fertilization. Gromax applied at the high rate produced a 74% increase in shoot volume after three growing seasons, whereas the organic amendment reduced volume by 28%. Growth was unaffected by mycorrhizal treatment. The growth response to the 16-g Gromax application probably reflected enhanced N, P, and K nutrition and decreased concentrations of potentially toxic metallic elements, including Mn and Al among others, as revealed through foliar analysis. Because they were accompanied by growth reduction, nutritional responses to the organic amendment, which involved both macronutrients and trace elements, were of little consequence. Impaired water relations may account for the poor response to this amendment. Likewise, nutritional responses to mycorrhizal inoculation produced no discernible benefit in terms of seedling performance. An inoculation procedure that failed to induce substantially greater P. tinctorius colonization in inoculated than uninoculated seedlings, and that may have also impaired water relations, likely explains this result. Overall, these findings indicate that further research is needed before either the organic amendment or the mycorrhizal inoculation procedure used here can be used in forest restoration efforts on dry sites.

Notizen: Abstract We compared the floristic composition and structure of restoration areas of eucalypt woodland with untreated pasture (control) and remnant vegetation (reference) in western Sydney. The restored areas comprised over 1,000 ha of abandoned pasture, which had been treated to reduce weeds and planted with seedlings of 26 native plant species raised from seed obtained locally from remnant vegetation. Plantings were carried out 0–9 years ago. Floristic composition was measured in quadrats using frequency scores and cover abundance. As far as possible treatments and restoration ages were replicated across sites. Ordination and analyses of similarity failed to distinguish the composition of restored vegetation from that of untreated pasture, which were both significantly different from that of remnant vegetation. There was a weak compositional trend with age of restored vegetation, but this was not in the direction of increasing resemblance to remnant vegetation. There was some evidence for convergence in structural features of restored with remnant vegetation, but this was at least partly attributed to plant growth. Subject to constraints imposed by the sampling design, environmental factors, and spatial variation were discounted as explanations for the results. The results therefore suggest either failure of restoration treatments or a restoration trajectory that is too slow to detect within 10 years of establishment. Our conclusions agree with those of similar studies in other ecosystems and support: (1) the need to monitor restoration projects against ecological criteria with rigorous sampling designs and analytical methods, (2) further development of restoration methods, and (3) regulatory approaches that seek to prevent damage to ecosystems rather than those predicated on replacing losses with reconstructed ecosystems.

Notizen: Abstract Landslides result in the loss of vertical vegetative structure, soil nutrients, and the soil seed bank. These losses impede timely recovery of tropical forest communities. In this study we added bird perches to six Puerto Rican landslides with three types of surfaces (bare, climbing fern, grass) in an effort to facilitate inputs of forest seeds through bird dispersal and to accelerate plant succession. Numbers of bird-dispersed forest seeds were significantly higher in plots beneath introduced perches than in control plots. Perches did not increase forest seedling densities compared with control plots. Seven different species of birds were observed on introduced perches. Because 99% of the seed inputs to controls and perch plots in the six landslides were wind-dispersed seeds (mostly graminoids), perches can improve landslide restoration if woody plants establish and shade out the dominant graminoid and climbing fern ground cover. Although increasing seed inputs from forest species is a critical step in accelerating revegetation of landslides, we suggest that supplemental restoration techniques be applied in addition to bird perches to promote forest recovery.

Notizen: Abstract The effects of seasonal drought, belowground competition, and low soil fertility on the survival and growth over 2 years of four native tree species planted on a degraded hillside grassland in Hong Kong were studied in a field transplant experiment using three-way analysis of variance. The tree species were Schima superba (Theaceae), Castanopsis fissa (Fagaceae), Schefflera heptaphylla (Araliaceae), and Sapium discolor (Euphorbiaceae), and the treatments were dry season irrigation, herbicide, and fertilizer. Each species responded differently to the treatments. Sapium had a very low survival rate as a result of wind damage at the exposed study site. All three treatments significantly reduced the survival rate of Castanopsis seedlings, whereas herbicide reduced it for Sapium but increased it for Schefflera. The significant effects on seedling growth were all positive, except for a strong negative effect of herbicide on Castanopsis growth. Overall, the results suggest that all three factors—seasonal drought, belowground competition, and low soil nutrients—can significantly impair seedling growth on a degraded hillside site in Hong Kong but that their relative importance differs among species. The growth benefits of the three treatments were largest and most consistent for Schima, which as a mature forest dominant would be expected to be particularly sensitive to the environmental conditions on degraded open sites. This study highlights the fact that more systematic planting trials are needed to identify suitable native tree species for cost-effective reforestation on degraded hillsides in Hong Kong and South China.

Notizen: Abstract Prescribed fire is often used to restore grassland systems to presettlement conditions; however, fire also has the potential to facilitate the invasion of exotic plants. Managers of wildlands and nature reserves must decide whether and how to apply prescribed burning to the best advantage in the face of this dilemma. Herbicide is also used to control exotic plants, but interactions between fire and herbicides have not been well studied. Potentilla recta is an exotic plant invading Dancing Prairie Preserve in northwest Montana. We used a complete factorial design with all combinations of spring burn, fall burn, no burn, picloram herbicide, and no herbicide to determine the effects of fire, season of burn, and their interaction with herbicide on the recruitment and population growth of P. recta over a 5-year period. Recruitment of P. recta was higher in burn plots compared with controls the first year after the fire, but this did not lead to significant population growth in subsequent years, possibly due to drier than normal conditions that occurred most years of the study. Effect of season of burn was variable among years but was higher in fall compared with spring burn plots across all years. Herbicide effectively eliminated P. recta from sample plots for 3–5 years. By the end of the study density of P. recta was greater in herbicide plots that were burned than those that were not. Results suggest that prescribed fire will enhance germination of P. recta, but this will not always lead to increased population growth. Prescribed fire may reduce the long-term efficacy of herbicide applied to control P. recta and will be most beneficial at Dancing Prairie when conducted in the spring rather than the fall. Results of prescribed fire on exotic plant invasions in semiarid environments will be difficult to predict because they are strongly dependent on stochastic climatic events.

Notizen: Abstract Boating activities are an increasing source of physical damage to coral reefs worldwide. The damage caused by ship groundings can be significant and may result in a shift in reef structure and function. In this study we evaluate the status of two restoration projects established in 1995, 6 years after two freighters, the M/V Maitland and the M/V Elpis, ran aground on reefs of the Florida Keys National Marine Sanctuary. Our approach includes field monitoring in support of simulation model development to assess the effectiveness of the restoration efforts. A population model was developed for the coral Porites astreoides to project the convergence rates of coral abundance and population size structure between the restored and surrounding reference habitats. Coral communities are developing rapidly on the restoration structures. Species richness and abundance of the dominant coral, P. astreoides, were nearly indistinguishable between the restoration structures and reference habitats after only 6 years. However, although abundance and size structure of P. astreoides populations are rapidly approaching those of the reference habitats (a convergence in size structure within 10 years was simulated), maximum coral size will take twice as long to converge for this species. The sensitivity of the model to maximum recruitment rates highlights the importance of recruitment on the recovery rates of restored habitats, suggesting that special attention should be afforded to provide coral recruits with appropriate recruitment substrate at the time of restoration. Finally, the rates of convergence and, hence, the level of success of a restoration effort were shown to be influenced not only by the recruitment and survivorship rates of corals on the restoration structures but by the characteristics of the reference population as well. Accordingly, reference populations ought to be considered a “moving target” against which restoration success has to be measured dynamically. The simple, cost-effective, monitoring–modeling approach presented here can provide the necessary tools to assess the current status of a restoration effort and to project the time required for coral populations to resemble those found on undamaged reference habitats

Notizen: Abstract Uniola paniculata (sea oats) rhizomes uprooted by hurricanes and deposited as wrack could be salvaged and replanted in dune restoration. To test this unexplored technique, percent tiller emergence was observed for 4 years from U. paniculata rhizomes replanted after submersion in seawater; air exposure of 0, 1, 3, 5, 7, and 11 days; freshwater rinse; and reburial in pots (watered) or on the beach (with or without supplemental water). In addition, U. paniculata rhizomes uprooted by Hurricane Georges were experimentally planted, and effects of soil salinity and moisture on emergence were tested in the greenhouse. Tiller emergence declined with increasing length of air exposure and decreasing size of rhizome. Tiller survival was enhanced by rainfall, rinsing with salt or fresh water during exposure and immediately after planting or supplemental beach watering. Although emergence was not reduced by soil salinity of 1,800 μS/cm, emergence was reduced by soil salinity of 5,800 μS/cm. Across the 4 years of the study tiller emergence from treated rhizomes varied considerably. U. paniculata rhizomes lost bud viability after 3–5 days of beach exposure, unless fresh water from rainfall, wet burlap storage, or watering was applied within 3 days. Bud viability was extended through 11 days when supplied with water. Thus, reburial within 3–11 days after a storm is a viable restoration technique.

Notizen: Abstract Annual grasslands in California are often managed with seasonal grazing and prescribed burning on the assumption that such practices have long-term benefits for native species. Mature native perennial bunchgrasses, particularly Nassella pulchra (purple needlegrass), are often the focal species, although very little is known about responses at different life history stages. Thus, important questions remain about long-term population dynamics of both mature plants and seedling recruitment. In plots receiving repeated grazing and burning events over 7 years, mortality of mature plants was threefold higher on mounds than on intermounds and likely reflected increased competition intensity associated with increased resource availability in deeper soil. Burning and grazing treatments had strong positive effects on basal area of mature N. pulchra. However, plants in grazed plots that were not burned contained considerable standing dead biomass. Topographic location strongly influenced growth as intermound plants grew relatively more than mound plants, but the effects on growth of burning and grazing did not vary with topographic location. In mapped plots N. pulchra recruitment was very low, and overall density dropped an average of 31%. However, a significant time-by-burning effect indicated that survival was significantly higher in burned plots. After 7 years of repeated treatments, effects of burning and grazing management on mature N. pulchra were positive but not for all phenological stages. Understanding long-term influence of management on bunchgrass populations may not be easy to determine because short-term results may not reflect long-term responses and some life cycle dynamics may be observed only over very long periods.

Notizen: Abstract Cades Cove, Great Smoky Mountains National Park, U.S.A. was historically cleared largely for pastoral purposes; it is now comprised of recently abandoned pastures dominated by non-native pasture species. To investigate the potential for reducing non-native species relative to native species, park managers initiated an experiment in 1995 that included mowing, herbicide application, planting of seed, and burning of replicate 20 × 50–m plots at each of two sites within Cades Cove. Between 1995 and 2001 we evaluated the response of the plant community (i.e., species-specific cover and frequency, biomass, diversity) to this suite of treatments and compared it with unmanipulated control plots at each site. Four years after treatment initiation abundance measures of Plantago lanceolata, Setaria geniculata, and Trifolium spp. averaged one-third lower in treated than control plots. Frequency of Festuca pratensis was lower in treated than in control plots for 2 years, but after 4 years its frequency, cover, and biomass did not differ between treated and control plots. By 2000 the cover of Sorghastrum nutans in treated plots increased to 23–47%, depending on the site. Total biomass and diversity increased in treated plots. The dominance of Lespedeza cuneata at one site apparently reduced planting success, biomass production, and diversity and evenness. Post-treatment lags in response for several species, coupled with interannual variation in response to environmental conditions, suggest that evaluations of treatment success would differ greatly depending on when the evaluation was conducted.

Notizen: Abstract We compared nekton and benthos densities and community compositions in a natural mixed seagrass bed dominated by Halodule wrightii (shoalgrass) with those found in three shoalgrass transplant sites and adjoining sand habitats in western Galveston Bay, Texas, U.S.A. Quantitative drop traps and cores were used to compare communities up to seven times over 36 months post-transplant where transplant beds survived. Total densities of fishes, decapods, annelids, benthic crustaceans, and most dominant species were significantly higher in natural seagrass than in transplanted shoalgrass or sand habitats during most sampling periods. On occasion, fish and decapod densities were significantly higher in transplanted shoalgrass than in adjoining sand habitats. No consistent faunal differences were found among transplant sites before two of three sites failed. Taxonomic comparison of community compositions indicated that nekton and benthos communities in natural seagrass beds were usually distinct from those in transplanted beds or sand habitats, which were similar. We conclude that reestablishing a shoalgrass bed that resembles a natural seagrass bed and its faunal communities in the Galveston Bay system will take longer than 3 years, provided that transplants persist.

Notizen: Abstract Restoration of degraded natural vegetation in parks is often complicated by the need to maintain public access. We tested whether the natural canopy species, Thuja occidentalis, can be restored to degraded cliff edges in Bruce Peninsula National Park, Ontario, Canada without reductions in visitor numbers. Eighty 10-year-old and 80 4-year-old container-grown saplings and 1,400 seeds were planted and monitored for 4 years. Eight treatments were applied that tested for effects of planting site (distance from cliff edge and pathways) and supportive measures (soil, water, cages, or signs) on survival, growth, and damage. No trees became successfully established from seed. Younger trees showed faster initial establishment and growth, but 4-year survival was the same for both age groups (39%). Supplemental soil improved the health of planted trees, and both soil and water slightly improved their survival. Cages did not affect survival and growth but decreased damage to 4-year-old trees and increased it for 10-year-olds. Signs had no effect on any measured variable. Trees planted away from the cliff edge and from pathways had the greatest establishment success, 4-year survival, and general health. Relative visitor density, as estimated from spot counts of visitors, had the largest effect on restoration success; the results suggest a threshold of visitor density above which restoration may be impossible. Planting location, especially with respect to shade, was also important. The planting of 4-year-old trees without supportive measures is suggested as the most cost-effective restoration technique at this site. The results indicate that restoration of open woodland habitats is possible without total visitor exclusion but that some restrictions on visitor numbers or activities are necessary.

Notizen: Abstract Ecological restoration enjoys widespread use as a technique to mitigate for environmental damage. Success of a restoration project often is evaluated on the basis of plant cover only. Recovery of a native arthropod fauna is also important to achieve conservation goals. I sampled arthropod communities by pitfall trapping in undisturbed, disturbed, and restored coastal sage scrub habitats in southern California. I evaluated arthropod community composition, diversity, and abundance using summary statistics, cluster analysis, and detrended correspondence analysis (DCA) and investigated influence of vegetation on arthropod communities with multiple regression analysis. Arthropod diversity at undisturbed and disturbed sites was greater than at sites that were 5 and 15 years following restoration ( p 〈 0.05). Number of arthropod species was not significantly different among undisturbed, disturbed, and restored sites, and two restoration sites had significantly more individuals than other sites. Vegetation at disturbed and undisturbed sites differed significantly; older restorations did not differ significantly from undisturbed sites in diversity, percent cover, or structural complexity. In multiple regression models, arthropod species richness and diversity was negatively related to vegetation height but positively related to structural complexity at intermediate heights. Exotic arthropod species were negatively associated with overall arthropod diversity, with abundance of the earwig Forficula auricularia best predicting diversity at comparison (not restored) sites (r2 = 0.29), and abundance of the spider Dysdera crocata and the ant Linepithema humile predicting diversity at all sites combined (r2 = 0.48). Native scavengers were less abundant at restored sites than all other sites and, with a notable exception, native predators were less abundant as well. DCA of all species separated restored sites from all other sites on the first axis, which was highly correlated with arthropod diversity and exotic arthropod species abundance. Lower taxonomic levels showed similar but weaker patterns, with example families not discriminating between site histories. Vegetation characteristics did not differ significantly between the newly restored site and disturbed sites, or between mature restoration sites and undisturbed sites. In contrast, arthropod communities at all restored sites were, as a group, significantly different from both disturbed and undisturbed sites. As found in other studies of other restoration sites, arthropod communities are less diverse and have altered guild structure. If restoration is to be successful as compensatory mitigation, restoration success standards must be expanded to include arthropods.

Notizen: Abstract The Pacific islands off southern California, U.S.A. and Baja California, Mexico hold potential for the conservation and restoration of California Mediterranean coastal ecosystems. However, the presence of exotic herbivores and invasive plants pose threats to these systems. Here, we use introduced herbivore removal as a large-scale experimental manipulation to examine the importance of top-down and bottom-up processes to a large-scale restoration effort. Using a paired approach on the Todos Santos Islands, Mexico we removed herbivores from one island, while they temporarily remained on an adjacent and similar island. We augmented this experiment with smaller scale herbivore exclosures on the control island. At both scales we failed to detect an herbivore effect on the plant community; rather plant community dynamics appeared to be dominated by El Niño related precipitation and exotic annuals. A parallel experiment on the San Benito Islands, Mexico revealed a different dynamic: Top-down effects on the plant community by exotic herbivores were evident. Differences in the response from the plant communities to both exotic herbivore presence and removal between these two island groups, along with Santa Barbara Island, U.S.A., where restoration has been on-going, raise important questions in ecosystem restoration. The history of anthropogenic disturbance, exotic plant abundance, and aridity play roles in postherbivore removal recovery. Although island conservation practitioners have honed the ability to remove exotic mammals from islands, development of invasive plant removal techniques is needed to fully capitalize on the conservation potential of California island ecosystems.

Notizen: Abstract Restoration practitioners have long been faced with a dichotomous choice of native versus introduced plant material confounded by a general lack of consensus concerning what constitutes being native. The “restoration gene pool” concept assigns plant materials to one of four restoration gene pools (primary to quaternary) in order of declining genetic correspondence to the target population. Adaptation is decoupled from genetic identity because they often do not correspond, particularly if ecosystem function of the disturbed site has been altered. Because use of plant material with highest genetic identity, that is, the primary restoration gene pool, may not be ultimately successful, material of higher order pools may be substituted. This decision can be made individually for each plant species in the restored plant community in the scientific context that ecosystem management demands. The restoration gene pool concept provides a place for cultivars of native species and noninvasive introduced plant material when use of native-site material is not feasible. The use of metapopulation polycrosses or composites and multiple-origin polycrosses or composites is encouraged as appropriate. The restoration gene pool concept can be implemented as a hierarchical decision-support tool within the larger context of planning seedings.

Notizen: Abstract Willamette Valley upland prairie in western Oregon, U.S.A. has been severely degraded and fragmented in the past 150 years after European settlement, resulting in vast population reductions of endemic species. Icaricia icarioides fenderi (Fender's blue butterfly) and Lupinus sulphureus ssp. kincaidii (Kincaid's lupine) are federally listed as Endangered and Threatened Species, respectively. Both are Willamette Valley upland prairie endemics, and Kincaid's lupine is the primary host plant for the Fender's blue butterfly. Attempts to grow Kincaid's lupine have been partially successful in a greenhouse situation; however, propagating plants from field-sown seed can be tenuous and plant establishment is unpredictable. Kincaid's lupine seeds were planted in the fall 1997 at two different upland prairie sites, and the cohort was followed through the summer 2000. Based on cohort tables the most vulnerable life stages to mortality are the germinant stage and the first growing year. Mechanical scarification of Kincaid's lupine seeds yielded no significant differences in survivorship, maternal function, plant size, and the percentage of seeds germinated compared with unscarified seeds. Differential seed source performance detected at one planting site suggests that underlying differences in population genetics may affect Kincaid's lupine vigor, fitness, and establishment. Future restoration projects for Kincaid's lupine should focus on upland prairie sites with naturally occurring lupine populations because local ecological conditions are favorable for lupine establishment. Moreover, the addition of new individuals to small Kincaid's lupine colonies will buffer against the effects of inbreeding depression and increase the site carrying capacity for Fender's blue butterfly.

Notizen: Abstract Rapid establishment by aggressive plants such as Phalaris arundinacea (reed canarygrass) often interferes with sedge meadow establishment in restored prairie pothole wetlands in the mid-continental United States. Introducing a cover crop during community establishment might suppress P. arundinacea invasion in restored prairie potholes by reducing resource availability. We evaluated two potential cover crops, Echinochloa crusgalli (barnyardgrass) and Polygonum lapathifolium (nodding smartweed), for suppressing P. arundinacea invasion in an experimental wetland using replacement series competition experiments. Further, we assessed the effects of E. crusgalli and P. lapathifolium on sedge meadow establishment by sowing Carex hystericina, a common wetland sedge, as a third species at a constant density in the replacement experiments. Echinochloa crusgalli, compared with no cover crop, reduced P. arundinacea biomass by more than 1,000 g/m2 (65%) after two growing seasons. Polygonum lapathifolium did not affect P. arundinacea biomass. Dense E. crusgalli canopies in the first year and thick E. crusgalli thatch in the second year substantially reduced light availability for P. arundinacea establishment. Echinochloa crusgalli also reduced C. hystericina biomass by more than 1,800 g/m2 (99%) after two growing seasons. Carex hystericina biomass was similar in plots sown with E. crusgalli to P. arundinacea monocultures. Neither E. crusgalli nor P. lapathifolium is likely to improve sedge meadow restoration success. These trends were not sensitive to initial sowing density or elevation above water level. Without methods to suppress P. arundinacea invasions, sedge meadow restorations may often fail. Thorough site preparation to remove P. arundinacea propagule sources before restoration is essential.

Notizen: Abstract Interest in restoring native ecosystems is resulting in conversion of marginal agricultural lands to bottomland hardwood-dominated forests in the midwestern and midsouthern United States. Growing stock for these efforts typically consists of planted oak (Quercus spp.) and volunteer vegetation. Reports of mixed reforestation success and the lack of post-establishment tree growth data prompted this evaluation of vegetation characteristics of 5- to 7-year-old operational restorations in the Lower Cache River Watershed in southernmost Illinois, U.S.A. Fraxinus pennsylvanica (green ash), Acer negundo (box-elder), and Liquidambar styraciflua (sweetgum) together comprised 77% of all tree stems observed. Full stocking of overstory tree species can be expected to produce a closed canopy stand within 160 m of a forested edge, due primarily to the abundance of rapidly growing volunteer-origin trees. Planted oaks contributed minimally to total tree stocking but were present in sufficient numbers to eventually improve wildlife habitat, and therefore satisfied restoration objectives. Oak height was 23% greater when in the presence of a non-oak tree species. Herbaceous cover was dominated by Solidago gigantea (late goldenrod) and Juncus spp. (rushes). Solidago gigantea was associated with poor growth and low density of non-oak stems, whereas Juncus dudleyi (Dudley's rush) was associated with taller non-oak stems. These results suggest that the presence of volunteer-origin trees is crucial for the creation of full stand stocking that will result in rapid development of a closed canopy forest. Improved success of future reforestation efforts will require more intensive methods to establish adequate stocking beyond 160 m of a forest edge. Methods described here could be adapted for agricultural field to forest restorations in other regions to predict critical distances from volunteer seed sources within which supplemental planting would be unnecessary to meet tree stocking objectives.

Notizen: Abstract The decline and range reduction of sage grouse populations are primarily due to permanent loss and degradation of sagebrush–grassland habitat. Several studies have shown that sage grouse productivity may be limited by the availability of certain preferred highly nutritious forb species that have also declined within sagebrush ecosystems of the Intermountain West, U.S.A. The purpose of this study was to determine the suitability of three species of forbs for revegetation projects where improving sage grouse habitat is a goal. Species suitability was determined by evaluating the emergence, survival, and reproduction of Crepis modocensis, C. occidentalis, and Astragalus purshii in response to method of establishment (seeding or transplanting), site preparation treatment (burned or unburned), and microsite (mound or interspace) in an Artemisia tridentata ssp. wyomingensis vegetation association in south central Oregon. For seeded plants A. purshii had the lowest emergence (8%) of all three species. Both seeded Crepis species had similar overall emergence (38%). Significantly more Crepis seedlings emerged from shrub mounds in unburned areas (50%) than in any other fire-by-microsite treatment (33 to 36%). Approximately 10% more Crepis seedlings survived in mounds compared with interspaces. Nearly twice as many emerging Crepis seedlings survived in the burned areas as opposed to unburned areas (p 〈 0.01). This resulted in more plant establishment in burned mounds despite higher emergence in unburned mounds. Astragalus purshii seedlings also survived better in burned areas (p = 0.06) but had no differential response to microsite. Fire enhanced survival of both Crepis and A. purshii transplants (p = 0.08 and p = 0.001). We believe additional research is needed to improve A. purshii emergence before it will become an effective plant for restoring sage grouse habitat. Conversely, we conclude that these Crepis species provide a viable revegetation option for improving sage grouse habitat in south central Oregon.

Notizen: Abstract Vegetation samples from either spontaneously revegetated or technically reclaimed spoil heaps of different ages in one of the largest active brown coal mining districts in Europe were analyzed and compared. The spoil heaps are located in the northwestern part of the Czech Republic and represent ages between 0 and 45 years. Ordination analysis (detrended correspondence analysis) showed that vegetation on technically reclaimed spoil heaps developed in a different way than that on spontaneously revegetated ones. The latter exhibited much higher species diversity in the oldest stages with the number of species doubled that of technically reclaimed sites. The oldest stages were also more advanced along an expected successional gradient as indicated by the ordination. Accelerating the vegetation development by technical reclamation was only of temporal character, whereas spontaneous succession proceeded further over the longer time scale. Spontaneous succession is advocated as an inexpensive and easy alternative to technical reclamation, because it leads to a more natural and rich vegetation cover. Unfortunately, technical reclamation is still the only approach considered in the present reclamation activities for this region.

Notizen: Abstract Fauna serve a key role in many forest ecological processes. Despite this, few studies have considered long-term faunal recolonization after mining and rehabilitation of forest ecosystems. In the jarrah forest of southwestern Australia, permanent fauna monitoring sites have been established in bauxite mined areas rehabilitated in 1990 and in a range of representative unmined forest control sites. At each site mammals, birds, reptiles, and ants were surveyed in 1992, 1995, and 1998. The aims of the monitoring were to develop a better understanding of faunal recolonization trends, to produce recommendations for promoting fauna return, and to consider which techniques and fauna groups are best suited for monitoring recolonization. The results showed that successional trends varied between fauna groups. Generalist foraging mammals recolonized rapidly, whereas small predators took longer. Feral mice were initially abundant and then declined. Birds gradually recolonized, and after 8 years bird communities were very similar to those in unmined forest sites. Reptile species took longer, and after 8 years numbers of species remained lower than in unmined forests. Species richness and diversity of ants in 8-year-old rehabilitation were comparable with those of unmined forest in some rehabilitated sites but were lower in others. The composition of ant communities was still different from that of unmined sites. Ant species that only use disturbed forest declined rapidly in abundance as rehabilitation aged. The results suggest that although the rates of faunal recolonization will vary, with time most or all mammal, bird, reptile, and ant species should inhabit rehabilitated bauxite mines. The densities of many are likely to be similar to those in unmined forest, but for others it is too early to know whether this will be the case. Techniques for promoting fauna return are discussed. This study demonstrates that no single fauna group is suitable for use as an overall “indicator” of faunal recolonization; different fauna species and groups reflect different aspects of faunal succession.

Notizen: Abstract Endozoochory has proven to be a highly effective mechanism in the dispersal of viable seeds in Mediterranean grasslands. We studied the effect of cattle dung application on species richness, particularly on the reintroduction of species lost after abandonment. Sown and control plots were monitored for 3 years after dung sowing. We found a significant increase in small-scale richness, which may be attributed to the treatment, with the inclusion of species detected in the dung and in the grazed pasture. The differences in richness and floristic composition diminished over time. This experiment proves the potential utility of this treatment for the restoration of species richness in abandoned pastures, although supplementary steps are necessary, including further sowing and/or shrub cutting in subsequent years.

Notizen: Abstract Natural accumulation of wind-borne sediments within or around the canopies of plants plays an important role in the ecological and evolutionary dynamics of many coastal and desert ecosystems. The formation of such phytogenic mounds (nebkhas) creates patches that can strongly influence the spatial distribution of plant and soil resources. In land restoration of arid and semiarid environments it is important to study the potential role of such biological patchiness that may provide sites for coexistence of species with different life and growth forms. Our main objective was to test whether the nebkhas of a leguminous shrub, Retama raetam (white broom), promote restoration of herbaceous vegetation and soil in the degraded rangelands of northern Sinai. Vegetation and microclimatic and edaphic characteristics within the nebkhas, as well as within internebkha spaces, were compared for ungrazed and grazed sites. Abundance and richness of herbaceous plants were positively related to nebkha area, which explained more of the variance of abundance and richness in the grazed site than in the ungrazed one. Protection from grazing, especially on nebkhas, was associated with an increase in abundance and richness of herbaceous plants, improved soil microclimate, and increased soil fine particles and nutrient concentrations. The results suggest that management (in casu protection from grazing) of nebkhas of woody perennial shrubs changes rangeland conditions and improves the resource regulatory processes. Furthermore, nebkhas of unpalatable plants have the potential to preserve plant diversity in overgrazed plant communities, because they are effective in capturing and retaining water, soil materials, and propagules within and from nearby areas, resources that would otherwise be lost.

Notizen: Abstract Sustained-release fertilizer and two kinds of mulch treatments were tested to determine their effects on survival and growth of planted Chamaecyparis thyoides (Atlantic white cedar) on a sandy extremely nutrient-deficient site. Height, basal trunk diameter, dry weight, and concentrations of nitrogen (N), phosphorus (P), and potassium (K) were measured for each treatment. After two growing seasons, survival was very high in untreated controls (mean, 86%) and was not significantly increased by any treatment (significance was assessed at p = 0.05 throughout this work). Stem height and cross-sectional area doubled in unamended plots during the course of the study. Fresh mulch alone caused no additional increase in growth, compared with unamended plots. Decomposed mulch caused a slight but significant increase. Sustained-release fertilizer caused significant increases in height (threefold) and dry weight (approximately sixfold). Combined treatment with fertilizer and mulch gave significantly greater growth responses than did other treatments, increasing heights 4- to 5-fold, trunk cross-sectional areas 4-fold, and dry weights 11- to 21-fold over no-treatment controls. Tissue concentrations of N and P correlated with growth trends, with combinations of mulch and timed-release fertilizer providing the highest values. Though statistically different, the two mulch treatments were similar in their effects on tissue nutrient concentrations. When combined with fertilizer, undecomposed mulch stimulated increases in height and dry weight significantly more than did decomposed mulch. Thus, establishment of C. thyoides on low-nutrient sandy soils is improved by combined soil amendment with sustained-release nutrients and organic mulch.

Notizen: Abstract Many grassland restoration projects on former arable land face problems because early successional grassland species establish vigorously and persistently but late successional grassland species fail to establish. Differences in establishment characteristics of early and late successional species might provide an explanation for the failure of many late successional species to colonize grasslands on ex-arable land. I examined whether early and late successional species had different establishment rates in the initial years of a grassland succession, whether a specific establishment stage (seedling emergence, mortality or growth) could be identified as the key process controlling establishment, and what management would enhance the establishment of late successional grassland species. Seeds of three early and three late successional species were sown separately in ex-arable plots with bare soil, 1-year-old vegetation, and 2-year-old vegetation. Emergence, mortality, and seedling growth were monitored for 1 year. Early successional species established successfully in the bare soil plots but failed to establish in plots with 1- and 2-year-old vegetation. Late successional species showed either lower establishment rates in the younger succession stages or decreased establishment with succession that nevertheless resulted in significant establishment in the oldest plots. Seedling emergence proved to be the key factor determining the establishment pattern of early and late successional species. In absolute numbers, emergence of late successional species was, however, similar or higher than that of early successional species, even in the earliest succession stage. The poor establishment of late successional species on former arable land could therefore not be explained solely by differences in establishment characteristics between early and late successional grassland species. Competitive processes between early and late successional species later in the life cycle probably play an important role. The results do point out that establishment of late successional species can be promoted by creating vegetative cover from the start of the restoration effort.

Notizen: Abstract In Maputaland, South Africa vegetative and microclimatic changes on mined dunes drive the composition of the dung beetle fauna toward convergence with that in natural dune forest on unmined dunes. We assessed the pattern of these changes using a 23-year vegetational chronosequence on mined dunes, which passes from grassland (approximately 1 year) to open Acacia shrubland thicket to Acacia karroo-dominated woodland (approximately 9 years). Across this sequence, which represents successional stages in the restoration of dune forest, there was a sequential trend toward convergence in dung beetle species composition in both the entire species complement and, particularly, in shade specialist species. However, species abundance patterns showed a trend toward convergence only in early chronosequence Acacia woodland, followed by a decline in similarity between dung beetle assemblages of older Acacia woodland and unmined natural forest. This trend toward divergence was common both to the entire species complement, which includes widespread taxa, and to species endemic to Maputaland or the east coast. These trends in similarity and dissimilarity between dung beetle assemblages closely parallel the greater physiognomic and microclimatic similarity between early Acacia woodland and natural forest and the relative dissimilarity of older Acacia woodland. In conclusion, although percentage similarities between dung beetle assemblages of approximately 12-year woodland and natural forests were comparable with those between each natural forest stand, decline in similarity in older woodland stands suggests that lasting convergence in dung beetle species abundance will only be attained once the Acacia woodland is replaced by secondary natural forest.

Notizen: Abstract We investigated effects of fire frequency, seasonal timing, and plant community on patchiness and intensity of prescribed fires in subtropical savannas in the Long Pine Key region of Everglades National Park, Florida (U.S.A.). We measured patchiness and intensity in different plant communities along elevation gradients in “fire blocks.” These blocks were prescribed burned at varying times during the lightning season and at different frequencies between 1995 and 2000. Fire frequency, seasonal timing, and plant community all influenced the patchiness and intensity of prescribed fires. Fires were less patchy and more intense, probably because of drier conditions and pyrogenic fuels, in higher elevation plant communities (e.g., high pine savannas) than in lower elevation communities (e.g., long-hydroperiod prairies). In all plant communities fires became increasingly patchy and less intense as the wet season progressed and moisture accumulated in fuels. Frequent prescribed fire resulted in increased patchiness but a wider range of intensities; higher intensities appeared to result from regrowth of more flammable vegetation. Our study suggests that frequent early lightning season prescribed fires produce a wider range of post-fire conditions than less frequent late lightning season prescribed fires. Our study also suggests that natural early lightning season fires readily carried through pine savannas and short-hydroperiod prairies, but lower elevation long-hydroperiod prairies functioned as firebreaks. Natural fires probably crossed these firebreaks only during drier years, potentially producing large landscape-level fires. Knowledge of how patchily and intensely fires burn across a savanna landscape should be useful for developing landscape-level fire management.

Notizen: Abstract To characterize the altitudinal and successional trends in microbial biomass and to understand their role in soil nutrient dynamics during the aggradation phase (vegetation recovery) of abandoned shifting cultivation systems, we determined the soil properties and microbial C and N in jhum (slash-and-burn) cultivation systems at different altitudes and 1-, 7-, and 16-year-old fallow agricultural lands at lower and higher altitudes in the northeastern Indian hills. Density of ground vegetation was lower in the undisturbed forest than in the jhum fallows. In general, 1-year jhum fallow had greater herbaceous vegetation both at lower and higher altitudes. Although woody plants were observed in 7- and 16-year-old jhum fallows, their density was highest in the forest. Soil moisture, organic C, and total N also increased gradually with increasing altitude and progressive secondary succession. Soil pH showed a negative correlation with altitude (as also confounded by soil type) and fallow age. Both microbial C and N had a close correlation with altitude and fallow age. Contribution of microbial C to soil organic C was 2.0–2.6% and microbial N to total N 1.4–2.2% in jhum fields, 2.4–4.3% and 1.2–2.1%, respectively, in jhum fallows, and 2.5–2.9% and 1.6–1.9% in the forests. Microbial C and N showed a negative correlation with herbaceous plant density. Microbial biomass in the jhum fallows and forest stands had a positive relationship with woody vegetation. Along an altitudinal and/or successional gradient, microbial C and N were positively correlated with water-holding capacity, soil moisture, organic C, and total N and negatively correlated with soil pH. Microbial C and N were positively correlated with each other. Therefore, the study suggests that the altitudinal and successional dynamics of microbial C and N are linked to, among other properties, soil organic matter and total nitrogen contents in the soil during community development after land abandonment from shifting cultivation.

Notizen: Abstract Successful control of invasive exotic plants depends to a large degree on the regeneration potential of the target exotic species and other species that might also be influenced by the removal effort. In coastal grasslands of California, exotic French broom (Genista monspessulana) forms both dense stands aboveground and abundant seed banks belowground. Land managers attempt to reduce broom cover and the seed bank through prescribed burning, but before this study, no investigators had examined the effect of repeated burning on French broom and associated grassland species. We found that the soil seed bank of stands that were burned had fewer broom seeds than unburned areas but that repeated burning did not reduce the seed bank beyond what was observed after one fire. Fire also did not have any consistent effect on the seed banks of other grassland species. We also examined the relationship between broom stand age and seed bank size but did not find a strong relationship between them. Our data suggested that the broom seed bank stays constant or declines slightly with stand age. We did, however, find that nonbroom seed numbers decreased as broom stands aged. Our results suggest that fire does reduce the size of the broom seed bank and that control of broom need not be limited to only the youngest stands.

Notizen: Abstract Arid and semiarid ecosystems in the Mediterranean are under high risk of desertification. Revegetation with native well-adapted evergreen shrubs is desirable, but techniques for successful establishment of these species are not fully developed. Transplant shock is a key hurdle to plantation success. The application of a drought-preconditioning treatment during the last months of nursery culture is a potential technique for reducing transplant shock. This technique has been widely applied in boreal habitats and humid temperate areas. Three representative Mediterranean species (Pistacia lentiscus, Quercus coccifera, and Juniperus oxycedrus seedlings) were exposed to classic drought-preconditioning treatment consisting of reductions in the watering regime. The effects of preconditioning on seedling quality were assessed by cell water relationships (pressure–volume curves), minimal transpiration, leaf capacitance, chlorophyll fluorescence, and gas exchange. Moreover, seedlings were exposed to transplant shock (intense drought period) during which water potential (predawn and midday) and maximal photochemical efficiency were evaluated to establish seedling performance. Results showed that preconditioning did not affect cell water relationships and minimal transpiration in any of the three species. Preconditioned seedlings of P. lentiscus maintained higher water content during desiccating conditions as a consequence of an increase in leaf water content at full turgor. These changes allowed plants to maintain higher net CO2 assimilation rates and an elevated photosystem II status, facilitating an increase in drought survival. Preconditioning improved the performance of Q. coccifera and J. oxycedrus seedlings, but these two species were much less responsive than P. lentiscus seedlings. Finally, results suggest that sensitivity to drought preconditioning may be related to drought tolerance or avoidance strategy of each species. Drought-related strategies should be considered to optimize management scale preconditioning.

Notizen: Abstract Having evolved in an environment with large, severe, and frequent disturbances, including massive floods, fires, and impacts of extinct and extirpated fauna, woodland herbs may be adapted to such disturbance processes. Present lack of such disturbances may contribute to present rarity. We test the hypothesis that transplanting with disturbance simulation can be used to restore the threatened woodland herb, Hydrastis canadensis (goldenseal). Three disturbance-simulation treatments (soil turnover, fertilization, and both) and a control were randomly applied to 100 blocks in goldenseal habitat, and a single rhizome was transplanted into each treatment. Transplanting was effective with 85% of the transplants surviving, 41% flowering, and 34% fruiting; thus, transplanting may increase area of occupancy. Soil turnover alone and combined with fertilization caused a significant increase in plant size available to support production of fruit. Increased flower and significantly increased fruit production were also characteristic of soil-turned plots. Results support the hypothesis that some woodland herbs are rare due to lack of certain disturbance, call for consideration of soil disturbance as a potentially important and beneficial influence on woodland herbs regardless of light effects, and suggest that transplanting into soil-overturned plots may restore goldenseal. The assumption that undisturbed conditions are optimal may impede effective management of rare woodland flora, highlighting the need for a more flexible approach.

Notizen: Abstract The success of revegetation efforts in southwestern U.S. riparian meadows has been limited because natural recolonization is often poor and little is known about establishment of transplanted native meadow species such as sedges. To evaluate their potential use in riparian restoration, the survival and growth of transplanted wildlings of three sedge species, Carex lanuginosa (woolly sedge), C. nebrascensis (Nebraska sedge), and C. rostrata (beaked sedge), were assessed. Transplanting occurred during two seasons (summer and fall) using two transplant sizes (295 and 680 cm3) at seven montane meadow sites in Arizona. Survival was similar among species, but shoot numbers were greater for C. lanuginosa (12.7 shoots/wildling) compared with C. nebrascensis (5.5 shoots/wildling) and C. rostrata (7.9 shoots/wildling). Survival was significantly greater for the summer transplant season (55.1%) versus fall (24.1%). Survival and growth were greater for the larger transplant size (46.1% large vs. 33.0% small; 8.1 shoots/wildling large vs. 6.4 small). Wildling survival was related to depth to groundwater for each species. Survival was highest for C. lanuginosa (78.6%), C. nebrascensis (88.2%), and C. rostrata (64.3%) where the groundwater depth was −48 to −60, −28 to −47, and −8 to −27 cm, respectively. These results suggest that restoration will be most successful if transplanting occurs in summer, using large wildling transplants when under stressful conditions such as waterlogged or dry soils, and when species are planted at appropriate groundwater depths.

Notizen: Abstract Lowland temperate grasslands dominated by Themeda triandra (kangaroo grass) are an endangered ecosystem in southeastern Australia. Grass biomass must be removed frequently to maintain plant diversity, but few studies of the impacts of different biomass removal techniques have been undertaken, and no rapid monitoring schemes have been developed. Low species densities in many reserves (due to past stock grazing) make it difficult to assess the effects of management regimes on plant diversity. Management impacts could be assessed by planting indicator species in replicated enhancement plots and subjecting these plots to adaptive management trials. A protocol for selecting potential indicator species is described, based on a regional quadrat database, using clearly defined criteria. Potential indicator species need to be conspicuous, easy to identify and abundant in high quality diverse grassland remnants, to have relatively broad ecological tolerances, to occur in sites that are relatively species rich and have a comparatively low cover of dominant exotic species, to commonly persist at low densities in long-grazed reserves, to be responsive to changes in management, and to have been studied ecologically. Only three species from western Victorian grasslands satisfied these criteria: Calocephalus citreus (lemon beauty-heads), Chrysocephalum apiculatum (common everlasting), and Leptorhynchos squamatus (scaly buttons). All are widespread, herbaceous, hemicryptophytic daisies. Despite a number of caveats, the scheme has the potential to provide a more clearly focused framework for grassland ecosystem management than currently exists.

Notizen: Abstract A growth experiment with native plants in pots exposed to natural environmental conditions evaluates the use of sewage sludge as a soil amendment in restoration of a southern California salt marsh. Sludge containing desirable organic matter but also undesirable heavy metal contaminants was mixed with a readily available matrix soil to reduce metal concentrations to levels below legal limits for land applications of sludge. Soil nutrient analysis revealed expected increases in total nitrogen and total phosphorus content with increasing sludge concentration. Soil metals analysis, however, revealed decreases in metal content with increased sludge concentration, a trend evidently caused by higher than expected metal content in the matrix soil. Five artificial soil mixtures ranging from 0 to 70% sludge were accompanied by natural wetland soil controls. Pots containing these soils were placed into a natural salt marsh. The pots were then planted with two native salt marsh plant species, Salicornia virginica and Frankenia grandifolia. Aboveground biomass was harvested after 12 months. Plant growth displayed no obvious change with increasing sludge concentration. Over the concentration ranges used, increased nutrient content did not stimulate plant growth and increased metal content did not inhibit plant growth. Plants grew better in natural wetland soil than in artificial soil mixtures, a trend probably caused by the substantially finer texture and higher organic content of natural soil. All sludge treatments differed more from the natural soil than from each other, implying that within the ranges examined, soil texture and organic content exerted more influence on plant growth than did metal or nutrient concentration. These results suggest that incorporating this sewage sludge in the soil of the restored salt marsh will neither benefit nor harm the plants that will live there and that greatest plant growth will be achieved by mixing the sludge with a fine-grained matrix soil.

Notizen: Abstract Post-mining coastal dune rehabilitation north of Richards Bay, KwaZulu-Natal started 20 years before field work for the present study commenced. This resulted in the development of a known age sere of coastal dune forest succession. These rehabilitating areas are fragmented by roads that may act as ecological edges. To establish whether roads affect regenerating bird, millipede, and rodent assemblages, multivariate techniques were applied to test for existence of edge and core assemblages within seral stages representative of the coastal dune forest successional sere. Edge and core assemblages were identified for both the bird and millipede communities but not for the rodent community. Low rodent numbers may have concealed edge effects, but the absence of edge and core assemblages could also be ascribed to the absence of a forest core. In the bird community species composition, richness, density, and total number of species contributed to the identification of edge and core assemblages. Within seral stages the species composition of millipedes differed between the edge and core assemblages. However, if the site was the same age the number of species in edge and core assemblages was similar. The generality of the edge concept should be approached with caution when dealing with taxa comprising species with such diverse natural histories as in the present study. It should also be kept in mind that some species require forest interiors for survival.

Notizen: Abstract A population of Hemideina crassidens (the Wellington tree weta) was monitored over a 4-year period after the eradication of Rattus exulans (the Polynesian rat kiore) and Gallirallus australis australis (the South Island weka) from Nukuwaiata (Chetwode Islands), Pelorus Sound, New Zealand. A novel survey technique (entrance scores) was used in combination with a conventional technique (random searches for active weta) to measure changes in weta population parameters after the removal of predation pressure and to investigate impacts of exotic predators on tree weta. Tree weta density did not increase markedly over the 4-year period, but the proportion of active adults did increase. Weta were observed to move into larger and more crowded galleries (refuges), to occupy galleries closer to the ground, and to spend less time sitting in gallery entrances. It was concluded that endemic tree weta are well adapted to withstand some introduced vertebrate predators but are able to live a more “relaxed” lifestyle in the absence of this predation. The most significant change detected was in weta age structure, with adults increasing their proportion of the population.

Notizen: Abstract To determine the effects of sward height and gap size on the emergence and subsequent development of Trollius europaeus, seeds were introduced into artificially created gaps in mown and unmown wet meadow grassland in Ayr, Scotland. Emergence and establishment of nondormant seed (previously leached in running tap water and soaked in gibberellic acid) placed in a range of circular gaps (25 mm, 50 mm with and without root barrier, 100 and 200 mm diameter) were monitored over 305 days. Trollius demonstrated high field emergence (mean, 46% of viable seed sown) in this experiment. Emergence was not significantly affected by either sward height or gap size, although emergence was greater in mown swards. The critical factor determining subsequent survival of seedlings was slug predation rather than sward height or gap size. However, losses due to slug predation were significantly greater in unmown swards, resulting in more seedlings in mown swards. One hundred eleven days after sowing (September) seedling numbers had declined substantially in both unmown and mown swards, and by day 305 (April) seedlings were extinct in both sward types. The implications of the research for the restoration and management of T. europaeus are discussed.

Notizen: Abstract We used differences in soil carbon δ13C values between forested sites and grasslands dominated by the C4 grass Schizachyrium scoparium (little bluestem) to detect the presence of former grasslands in the historical landscape of the coastal sand plain of Martha's Vineyard, Massachusetts, U.S.A. Soil δ13C was measured at (1) sites with long-term forest or grassland vegetation and (2) sites with known histories where forest vegetation invaded grassland and where forest converted to grassland. The δ13C of soil under long-term grassland was –24.1‰ at 0 to 2 cm depth and –23.4‰ at 2 to 10 cm and was enriched by 3.4‰ and 2.8‰ compared with soil under long-term forest. In forests that invaded grasslands dominated by S. scoparium, soil δ13C decreased as C derived from trees replaced C from S. scoparium. This decline occurred faster in surface soils and in the light soil organic matter fraction than in the mineral soil. In forests that converted to grasslands, soil δ13C increased and the rate of increase was similar in surface and mineral soil and in the different soil organic matter fractions. Rates of change indicated that soil δ13C could be used to detect changes in vegetation involving the presence or absence of S. scoparium during the last 150 years. Application of this model to a potential grassland restoration site on Martha's Vineyard where the landscape history was not known indicated that the site was previously unoccupied by S. scoparium during this time. The δ13C of surface mineral soil can be useful for detecting the presence of historic S. scoparium grasslands but only in the period well after European settlement of these coastal sand plain landscapes.

Notizen: Abstract Coral reef degradation has been widely reported for the past 20 years. Because the recovery rate is usually low, various methods of restoration have been explored in different regions of the world. Among the effective and commonly used methods to restore coral communities is the transplantation of coral colonies or fragments. In this investigation fragments of Acropora pulchra were used in a semiprotected nursery in southern Taiwan between 1996 and 1998 to test, in situ, the possible effects of different factors on the generation of new branches and the initial skeletal extension rates of transplants. The variables under study here were the origin and length of the fragments, their new orientation, presence of tissue injury, and position in the fragment. All these factors were found to make a difference in either one or both aspects of coral growth (i.e., branching frequency and skeletal extension rate). These two factors clearly determine the success rate of a small fragment developing into a large colony that has a much higher probability to survive and grow on its own. It is now obvious that the efficiency of coral generation through fragment culture can be enhanced if the variables examined here are taken into consideration. Once coral colonies are formed, they can be fragmented again to generate more corals or can be transplanted to a suitable site.

Notizen: Abstract Historic fire return intervals in Artemesia tridentata (big sagebrush) ecosystems have been altered by livestock grazing, fire suppression, and other land management techniques resulting in ecological changes in these areas. Increases in abundance of woody vegetation may be causing declines in native herbaceous understory species. We examined the effects of prescribed fire on the morphology, abundance, and phenology of nine abundant forb (herbaceous dicot) species used selectively by Centrocercus urophasianus (Sage Grouse). In September 1997 prescribed fire was applied to four of eight randomly assigned 400-ha A.t. wyomingensis (Wyoming big sagebrush) study plots at Hart Mountain National Antelope Refuge, Oregon. Livestock had not grazed experimental plots since 1991. Burning caused morphological changes such as significantly greater numbers of racemes and flowers per raceme in Astragalus malachus (shaggy milkvetch-Legumoideae) (9 in burn vs. 6 in control; 23 in burn vs. 21 in control, respectively). Also, prescribed burning caused greater numbers of flowers in Phlox gracilis (microsteris-Polemoniaceae) (57 vs. 13), greater numbers of umbels and umbelletts in Lomatium nevadense (Nevada desert parsley-Umbellifereae) (4 vs. 2 and 59 vs. 31, respectively), greater numbers of flower heads in Crepis modocensis (Modoc hawksbeard-Compositae) (32 vs. 21), and greater number of flowers/cm3 in Phlox longifolia (longleaf phlox-Polemoniaceae) (0.11 vs. 0.06). Crown volume of Crepis modocensis (7,085 vs. 4,179 cm3) and Astragalus malachus (2,854 vs. 1,761 cm3) plants was greater in burned plots than control plots. However, burning resulted in a smaller crown area of Antennaria dimorpha (low pussytoes-Compositae) (20 vs. 37 cm2). Phenology and time of flowering were also affected by fire. The period of active growth for each species was extended later into the summer in burned plots ( p 〈 0.01). In addition, Crepis modocensis and Lomatium nevadense flowered 12 to 14 days earlier in burned plots. Fire had no effect on frequency, density, and relative abundance of seven of the nine studied species. Fire reduced the frequency and relative abundance of A. dimorpha and Phlox longifolia and reduced the density of A. dimorpha.

Notizen: Abstract Since 1990 under the Eastern Habitat Joint Venture over 100 small wetlands have been restored in Prince Edward Island, Canada. Wetlands were restored by means of dredging accumulated sediment from erosion to emulate pre-disturbance conditions (i.e., open water and extended hydroperiod). In 1998 and 1999 we compared waterfowl pair and brood use on 22 restored and 24 reference wetlands. More pairs and broods of Ring-necked Ducks, Gadwall, Green-winged Teal, and American Black Ducks used restored versus reference wetlands. In restored wetlands waterfowl pair density and species richness were positively correlated with wetland/cattail area, percent cattail cover, and close proximity to freshwater rivers. In addition, a waterfowl reproductive index was positively correlated with percent cattail cover. Green-winged Teal pair occurrence in restored wetlands was positively correlated with greater amounts of open water and water depths. American Black Duck pairs occurred on most (86%) restored wetlands. Restored small wetlands likely served as stopover points for American Black Duck broods during overland or stream movements, whereas they likely served as a final brood-rearing destination for Green-winged Teal broods. We suggest that wetland restoration is a good management tool for increasing populations of Green-winged Teal and American Black Ducks in Prince Edward Island.

Notizen: 1 Our previous report showed that in acute cholestasis, the subsensitivity to morphine inhibitory effect on electrical-stimulated contractions develops significantly faster in guinea-pig ileum (GPI) and in mouse vas deferens (MVD) (45.2 and 29.9 times, respectively) compared with non-cholestatic subjects. 2 The possible contribution of α2-adrenoceptor and nitric oxide (NO) pathways on the development of tolerance was assessed in GPI and MVD of cholestatic subjects. 3 Daily administration of naltrexone (20 mg kg−1), yohimbine (5 mg kg−1), and Nω-nitro-l-arginine methyl ester (l-NAME) (3 mg kg−1) to cholestatic animals significantly (P-value 〈 0.05) inhibited the process of subsensitivity in all groups. 4 Consistent with the literature, it was concluded that both the α2-adrenergic system and NO have close interaction with the opioid system and may underlie some of the mechanisms involved in the subsensitivity development to opioids in acute cholestatic states.

Notizen: 1 We examined the role of the NO/cyclic GMP (cyclic GMP) pathway in nitric oxide (NO)- and vasoactive intestinal peptide (VIP)-induced relaxation of feline lower oesophageal sphincter (LES). Furthermore, it was studied whether methylene blue, LY83583 and ODQ, which are soluble guanylate cyclase (sGC) inhibitors, could inhibit NO-induced relaxation.2 The nitric oxide synthase (NOS) inhibitor, N-nitro-l-arginine (l-NNA) had no effect in sodium nitropruside (SNP)-induced relaxation, but 3-morpholinosydnonimine-N-ethylcarbamide (SIN-1)-induced relaxation was decreased by the pretreatment of l-NNA, which showed that SIN-1, not SNP, could activate NOS to cause relaxation. Methylene blue and LY83583 did not inhibit the relaxation by SNP and SIN-1. However, the more specific sGC inhibitor ODQ blocked the relaxation induced by NO donors.3 To identify the relationship of NOS, sGC and adenylate cyclase in VIP-induced relaxation, tissue were pretreated with l-NNA and ODQ and SQ22536. These inhibitors produced significant inhibition of this response to VIP. The adenylyl cyclase inhibitor SQ 22536 also inhibited relaxation by VIP.4 In conclusion, our data showed that SNP- and SIN-1-induced relaxation was mediated by sGC. Of sGC inhibitors, methylene blue and LY83583 were not adequate for the examination of NO donor-induced feline LES smooth muscle relaxation. VIP also caused relaxation by the pathway involving NO and cGMP and cAMP.

Notizen: 1 The vasoconstrictor response to periarterial nerve electrical stimulation (PNS) and neurotransmission by ATP are discussed and illustrated, using canine isolated and perfused splenic arterial preparations. 2 The conditions for appearance of dominant purinergic constrictor response to PNS are discussed. 3 Modulation of the purinergic vasoconstrictor responses to PNS by several kinds of presynaptic receptor agonists and antagonists is reviewed. 4 Influences of purinergic responses to PNS by guanethidine, reserpine, tetrodotoxin (TTX) or ω-conotoxin GVIA (ωCTX) are also reviewed. 5 Effects of imipramine and removal of the endothelium are discussed. 6 Evidence is presented for selective inhibition of purinergic responses to PNS by an adequate cold storage of the vessel. 7 The roles of ATP released by PNS in isolated canine splenic arteries are proposed.

Notizen: 1 The aim of the present study was to investigate the role of several possible neurotransmitters in mediating non-adrenergic, non-cholinergic (NANC) relaxation, and the effects of phosphodiesterase (PDE) III and V inhibitors on adrenergic and NANC relaxation in branch pulmonary artery (PA) of guinea-pig. 2 Under the NANC conditions, electrical field stimulation (EFS, 60 V, 0.2 ms, 20 Hz) induced a tetrodotoxin-sensitive relaxation of the histamine-precontracted PA rings. The nitric oxide (NO) synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME, 10−4 m) and the guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 10−5 m) partially inhibited the EFS-induced relaxation. The inhibitory effect of l-NAME was reversed completely by l-arginine (10−3 m), but not d-arginine (10−3 m). 3 This NANC relaxation was attenuated by 8-phenyltheophylline (10−5 m), a P1-purinoceptor antagonist. 4 The NANC response was potentiated by 10−6 m zaprinast, a type V PDE inhibitor, but was unaffected by 3 × 10−6 m milrinone, a type III PDE inhibitor. 5 Sodium nitroprusside (SNP) caused a concentration-dependent vasodilator effect which was potentiated by zaprinast, but unaffected by milrinone. Moreover, the effect of combination of zaprinast with milrinone was not significantly different from that observed with zaprinast alone. 6 Isoprenaline produced a concentration-dependent vasodilatation in branch PA of guinea-pig which was potentiated by both zaprinast and milrinone, the efficacy of milrinone being greater than zaprinast. 7 These results suggest that both nitrergic and purinergic pathways are involved in mediating the NANC relaxation in branch PA of guinea-pig. The combination of PDE III or V inhibitors with vasorelaxant drugs may be a hopeful approach for the treatment of pulmonary hypertension.

Notizen: 1 The present survey is dealing with the interactions between the renin–angiotensin–aldosterone system (RAAS) and the sympathetic nervous system (SNS) in various organs and tissues, with an emphasis on the angiotensin AT-receptors located at the sympathetic nerve endings. 2 Angiotensin II, the main effector of the RAAS is known to stimulate sympathetic nerve traffic and its sequelae in numerous organs and tissues, such as the central nervous system, the adrenal medulla, the sympathetic ganglia and the sympathetic nerve endings. These stimulatory effects are mediated by AT1-receptors and counteracted by AT1-receptor antagonists. 3 Sympatho-inhibition at the level of the sympathetic nerve ending appears to be a class effect of the AT1-receptor blockers, mediated by presynaptic AT1-receptors. With respect to the ratio pre-/postsynaptic AT1-receptor antagonism important quantitative differences between the various compounds were found. 4 Both the pre- and postjunctional receptors at the sympathetic nerve endings belong to the AT1-receptor population. However, the presynaptic receptors belong to the AT1B-subtype, whereas the postjunctional receptors probably belong to a different AT1-receptor subpopulation. 5 Sympatho-inhibition is a class effect of the AT1-receptor antagonists. In conditions in which the SNS plays a pathophysiological role, such as hypertension and congestive heart failure, this property may well be of therapeutic relevance.

Notizen: 1 The psychostimulant constituent of khat leaves, S-(−)-cathinone, was examined for vascular activity on the coronary vasculature of guinea-pig-isolated perfused hearts and aortic ring preparations. 2 Cathinone caused coronary vasoconstriction, negative inotropy and negative chronotropy in isolated hearts. The major metabolite of cathinone after its ingestion, 1R.2S-(−)-norephedrine (norephedrine), also caused coronary vasoconstriction comparable with that by cathinone. Norephedrine, however, had no effect on force or rate of cardiac contractions. 3 Cocaine (10 μm) potentiated the coronary vasoconstriction and positive inotropy by noradrenaline indicating inhibition of neuronal uptake. The vasoconstriction and negative inotropy by cathinone, however, were not affected, indicating that its action was not via release of noradrenaline from sympathetic neurones. 4 The α1-adrenoceptor antagonist, prazosin, blocked the vasoconstriction by noradrenaline, but not that produced by cathinone in the presence of cocaine. This indicates that the coronary vasoconstriction by cathinone was not due to an action on α1-adrenoceptors either directly or indirectly through noradrenaline release. 5 Three repeated doses of cathinone displayed the same coronary vasoconstrictor responses, indicating a lack of tachyphylaxis and therefore confirming that the response was unlikely to be due to indirect sympathomimetic activity through release of noradrenaline. 6 In guinea-pig aortic rings, the order of vasoconstrictor activity was: noradrenaline 〉 norephedrine 〉 cathinone, with each causing approximately equivalent maximum responses. The time to reach plateau contractions was shortest for noradrenaline (5.1 ± 0.5 min), then norephedrine (9.3 ± 1.5 min) and cathinone the longest (25.4 ± 3.2 min, 335 μm dose). 7 These results indicate that cathinone has vasoconstrictor activity which is not due to indirect or direct sympathomimetic activity. The precise mechanism for this vasoconstriction remains to be determined. The coronary vasoconstriction may explain the increased incidence of myocardial infarction in khat chewers, which may arise from coronary vasospasm.

Notizen: 1 A variety of prostanoids were examined for their ability to alter the periarterial nerve stimulation-induced release of noradrenaline (NA) and neuropeptide Y immunoreactive compounds (NPY-ir) from the perfused mesenteric arterial bed of the rat. 2 Periarterial nerve stimulation (16 Hz) increased the overflow of NA, NPY-ir and perfusion pressure. 3 The prostacyclin (PGI2) analogues, carbaPGI2 and cicaprost both produced a concentration-dependent attenuation of the nerve stimulation-induced increase in NA, NPY-ir overflow and perfusion pressure. 4 The prostaglandin (PG) analogue PGE2 attenuated the evoked increase in NPY-ir overflow as well as a modest decrease in NA. 5 PGE1, sulprostone and iloprost attenuated the nerve stimulation-induced increase in NA overflow but not NPY-ir. 6 Neither PGF2α nor the thromboxane A2 analogue U46619 altered the evoked increase in NA or NPY-ir overflow. 7 The results support the view that sympathetic co-transmitter release can be differentially modulated by paracrine/autocrine mediators at sympathetic neuroeffector junctions.

Notizen: 1 Experiments were undertaken to determine if the imidazoline/α2-adrenoceptor agonist, rilmenidine, would produce mydriasis in cats and, if so, to delineate its site of action and determine if this effect is mediated by imidazoline receptors or α2-adrenoceptors. 2 Rilmenidine produced dose-related pupillary dilator responses in pentobarbital anaesthetized cats that were independent of sympathetic innervation to the iris but were dependent upon intact parasympathetic neuronal tone. The ED50 for rilmenidine-induced pupillary dilation was approximately 200 μg kg−1, i.v., and was sustained for at least 1 h. 3 The highly selective α2-adrenoceptor antagonist, RS-79948, administered either before or after rilmenidine, antagonized rilmenidine-induced mydriasis. Neuronally induced reflex inhibition of parasympathetic nerve activity was also inhibited by administration of RS-79948. 4 These results suggest that rilmenidine acts like clonidine to produce pupillary dilation by inhibition of parasympathetic tone to the iris sphincter and that this central nervous system parasympatho-inhibition is mediated by α2-adrenoceptors, rather than imidazoline receptors.

Notizen: 1 Increasing evidence indicates the occurrence of functional interconnections between immune and nervous systems, although data available on the mechanisms of this bi-directional cross-talking are frequently incomplete and not always focussed on their relevance for neuroimmune modulation.
2 Primary (bone marrow and thymus) and secondary (spleen and lymph nodes) lymphoid organs are supplied with an autonomic (mainly sympathetic) efferent innervation and with an afferent sensory innervation. Anatomical studies have revealed origin, pattern of distribution and targets of nerve fibre populations supplying lymphoid organs.
3 Classic (catecholamines and acetylcholine) and peptide transmitters of neural and non-neural origin are released in the lymphoid microenvironment and contribute to neuroimmune modulation. Neuropeptide Y, substance P, calcitonin gene-related peptide, and vasoactive intestinal peptide represent the neuropeptides most involved in neuroimmune modulation.
4 Immune cells and immune organs express specific receptors for (neuro)transmitters. These receptors have been shown to respond in vivo and/or in vitro to the neural substances and their manipulation can alter immune responses. Changes in immune function can also influence the distribution of nerves and the expression of neural receptors in lymphoid organs.
5 Data on different populations of nerve fibres supplying immune organs and their role in providing a link between nervous and immune systems are reviewed. Anatomical connections between nervous and immune systems represent the structural support of the complex network of immune responses. A detailed knowledge of interactions between nervous and immune systems may represent an important basis for the development of strategies for treating pathologies in which altered neuroimmune cross-talking may be involved.

Notizen: 1 Calcium/calmodulin-dependent protein kinase II (CaMKII) has an important function in mediating insulin release but its role in the development of diabetes-induced cardiovascular complications is not known. 2 We investigated the ability of a chronic administration of KN-93 (5 mg kg−1alt diem for 4 weeks), an inhibitor of CaMKII, to modulate the altered vasoreactivity of the perfused mesenteric bed to common vasoconstrictors and vasodilators in streptozotocin (STZ)-induced diabetes. 3 The vasoconstrictor responses induced by noradrenaline (NE), endothelin-1 (ET-1), and angiotensin II (Ang II), were significantly increased whereas, vasodilator responses to carbachol and histamine were significantly reduced in the perfused mesenteric bed of the STZ-diabetic rats as compared with non-diabetic controls. 4 Inhibition of CaMKII by KN-93 treatment did not affect blood glucose levels but produced a significant normalization of the altered agonist-induced vasoconstrictor and vasodilator responses. KN-93 did not affect agonist-induced responses in control animals. In addition, KN-93 significantly reduced weight loss in diabetic rats. 5 The present data suggest that CaMKII is an essential mediator in the development of diabetic vascular dysfunction and may also play an important role in signalling pathways leading to weight loss during diabetes.

Notizen: 1 In the present investigation we examined the regulation of calmodulin (CaM)- and protein kinase C (PKC)-dependent pathways by cytosolic Ca2+ in the contraction of cat lower oesophageal sphincter (LES). 2 Force developed in response to increasing doses of acetylcholine (ACh) was directly related to the increase of the [Ca2+]i measured by fura-2. Thapsigargin, which depletes Ca2+ stores, reduced the contraction and the [Ca2+]i. In addition, contraction in response to maximal ACh was reduced by the CaM inhibitor CGS9343B but not by the PKC inhibitor chelerythrine. The contraction in response to submaximal ACh was reduced by chelerythrine but not by CGS9343B. 3 In permeabilized cells, the contraction in response to low Ca2+ (0.54 μm) was also reduced by CGS9343B. 4 The response to high Ca2+ (1.0 μm) was reduced by CGS9343B. ACh also inhibited PKC activation induced by diacylglycerol, which activation is inhibited by the N-myristoylated peptide inhibitor derived from pseudosubstrate sequences of PKCαβγ (myr-PKC-αβγ), but not of myr-PKC-α. 5 These data are consistent with the view that activated CaM-dependent pathways inhibit PKC-dependent pathways, this switch mechanism might be regulated by Ca2+ in the LES.

Notizen: 1 Approximately 45% of patients with diabetes mellitus have gastrointestinal complications such as diarrhoea and constipation, but the underlying aetiology is unclear. The present study investigates alterations in spontaneous motility of the colon that may be, in part, responsible for these symptoms using an established animal model of diabetes.2 Rats were rendered diabetic by a single intraperitoneal injection of streptozotocin and age-matched controls were injected with citrate buffer. Rats were sacrificed after 8-weeks and proximal colonic circular muscle tissue mounted in organ baths.3 Spontaneous activity was observed in both control and diabetic tissues, but this activity was almost doubled in colonic tissue taken from diabetic rats. It was hypothesized that this increase was due to a deficit in inhibitory control of the colon in the diabetic state.4 Possible alterations in nitrergic and vasoactive intestinal polypeptide (VIP)ergic control were investigated using a range of pharmacological tools.5 Sodium nitroprusside, VIP and antioxidants (reduced glutathione, ascorbate and α-tocopherol) inhibited the spontaneous activity, but the level of inhibition observed was not different in diabetic tissue compared with control.6 Arginine, [d-p-Cl-Phe6, Leu17]-VIP and α-chymotrypsin had no effect on spontaneous activity in either sets of tissue.7 N-nitro-l-arginine produced a small, but significant, increase in the level of spontaneous activity, but the degree of increase was not different between control and diabetic tissues.8 Western blots demonstrated that there was no inducible-nitric oxide synthase (iNOS) in control or diabetic tissues and that the levels of endothelial-NOS (eNOS) and neuronal-NOS (nNOS) detected were not statistically significantly different. The [3H]-citrulline assay established that the functionality of the NOS isoforms present were unaltered in the diabetic state.9 This study demonstrates that there is a marked alteration in motility in the colon taken from diabetic animals. However, the change in motility is unlikely to be due to a change in inhibitory control mechanisms and may be due to an increased excitability.

Notizen: 1 Pig nasal mucosal strips were incubated with α-adrenoceptor antagonists followed by α2-adrenoceptor agonist concentration–response curves. 2 Contractions elicited by the α2-adrenoceptor agonists BHT-920 (pD2 = 6.16 ± 0.07), UK 14,304 (pD2 = 6.89 ± 0.13) and PGE-6201204 (pD2 = 7.12 ± 0.21) were blocked by the α2-adrenoceptor antagonist yohimbine (0.1 μm). In contrast, the α1-adrenoceptor antagonist prazosin (0.03 μm) had no effect on the BHT-920-, UK 14,304- and PGE-6201204-induced contractions, but blocked the contractile response to the α1-adrenoceptor agonist phenylephrine (pD2 = 5.38 ± 0.04) and the mixed α1- and α2-adrenoceptor agonist oxymetazoline (pD2 = 6.30 ± 0.22). 3 The α2-adrenoceptor antagonist yohimbine (0.01–0.1 μm, pA2 = 8.04), α2B/C-adrenoceptor antagonist ARC 239 (10 μm, pKb = 6.33 ± 0.21), α2A/C-adrenoceptor antagonist WB 4101 (0.3 μm, pKb = 8.01 ± 0.24), α2A-adrenoceptor antagonists BRL44408 (0.1 μm, pKb = 6.82 ± 0.34) and RX 821002 (0.1 μm, pKb = 8.31 ± 0.35), α2C-adrenoceptor antagonists spiroxatrine (1 μm, pKb = 7.32 ± 0.32), rauwolscine (0.1 μm, pKb = 8.16 ± 0.14) and HV 723 (0.3 μm, pKb = 7.68 ± 0.14) inhibited BHT-920-induced contractions in pig nasal mucosa. 4 The present antagonist potencies showed correlations with binding affinity estimates (pKi) obtained for these antagonists at the human recombinant α2A- and α2C-adrenoceptors (r = 0.78 and 0.83, respectively) and with binding affinity estimates (pKd) obtained in pig native α2A- and α2C-monoreceptor assays (r = 0.85 and 0.78, respectively). No correlation was observed for the α2B-subtype. 5 In conclusion, contractile responses to phenylephrine, BHT-920, UK 14,304, PGE-6201204 and oxymetazoline indicate that α1- and α2-adrenoceptors are present and mediate vasoconstriction in pig nasal mucosa. Furthermore, correlation analysis comparing antagonist potency in pig nasal mucosa with affinities for human recombinant α2-adrenoceptors and native pig α2-adrenoceptors suggest that α2A- and α2C-adrenoceptor subtypes constrict pig nasal mucosa vasculature.

Notizen: 1 The relationship between the effects of endogenous nitric oxide (NO) and prostanoids on the noradrenaline (NA)-induced contractions and the mechanisms involved were investigated in the rat perfused mesenteric bed, using NG-nitro-l-arginine methyl ester (l-NAME), a NO synthase inhibitor and sodium nitroprusside (SNP), a NO donor.2 The constrictor responses to NA were reduced to 50% by the cyclooxygenase inhibitor 10 μm indomethacin as well as by 1 μm SNP. When indomethacin and SNP were perfused simultaneously the contractions were further reduced.3 The NA-induced contractions were increased by the addition of 400 μml-NAME and the addition of either indomethacin or SNP abolished such increases. The simultaneous perfusion of both agents further reduced the contractions.4 Removal of the endothelium increased NA-induced contractions to a similar extent as l-NAME and this increase was abolished by indomethacin as well as by SNP.5 The perfusion of 10 μm NA augmented the release of prostaglandin (PG) F2α by the mesenteric bed without modifications in any other prostanoid. In the presence of l-NAME, this effect was further increased. However, SNP abolished the NA-induced stimulation of PGF2α release.6 In de-endothelialized preparations NA also stimulated PGF2α production as observed in intact preparations. This effect was more marked in the presence of l-NAME; in contrast, SNP abolished the stimulation.7 In conclusion, the present results suggest an opposite action between NO and PGF2α on the NA-induced contractions in the rat mesenteric bed.

Notizen: 1 The present study was carried out to characterize the effect of adenosine on calcium dynamics in the rat portal vein. Isolated portal vein of male albino rats was used as the experimental model as it exhibits autorhythmicity. Adenosine and its analogues 2-CAD, N6-CHA and NECA were used to characterize the type of adenosine receptor involved and 2-CAD was used along with adenosine throughout the other part of study to characterize the effect of adenosine on Ca2+ dynamics.2 Adenosine and its analogues were found to inhibit the spontaneous contractions of rat portal vein in a concentration-related manner. The order of potency was NECA 〉 2-CAD 〉 N6-CHA 〉 adenosine. Incubation of the tissue with CGS∼15943A, an adenosine receptor antagonist, had a per se enhancing effect on autorhythmicity. Adenosine and 2-CAD failed to reverse the contractile response produced by hypertonic KCl (80 or 30 mm). Whereas adenosine and 2-CAD effectively relaxed the tissues contracted with phenylephrine (10−5 m).3 Preincubation of the tissue with 2-CAD (10−4, 10−5 or 10−6 m) for 5 min raised the threshold concentration of CaCl2 to evoke contractile response and also significantly increased the mean EC50 values of CaCl2. Nifedipine was found to be more potent than 2-CAD on Ca2+ channels.4 The results of the present study suggest that the endogenous adenosine plays a significant role in producing vascular relaxation through the participation of A2 receptor subtype. This effect may be due to its inhibitory effect on release of Ca2+ from the intracellular stores. Further to this effect, 2-CAD had a major inhibitory effect on voltage-operated Ca2+ channels compared with receptor-operated Ca2+ channels.5 It can be concluded that adenosine through its A2 receptor produces vasorelaxant effect by interfering with the release of Ca2+ from the intracellular stores coupled with influx of Ca2+ from the extracellular sources.

Notizen: 1 The prolonged infusion of 1,3-dipropyl-8-sulfophenylxanthine (DPSPX), a non-selective antagonist of adenosine receptors, induces hypertension, an increase in plasma renin activity and morphological cardiovascular changes. 2 The aim of this work was to evaluate the effects of losartan, a selective AT1 receptor antagonist, and atenolol, a β-adrenoceptor antagonist, on DPSPX-induced hypertension. 3 Male Wistar rats (250–300 g, n = 4–6) were treated for 1 or 4 weeks with: saline i.p.; DPSPX (90 μg kg−1 h−1) i.p.; losartan (15 mg kg−1 day−1) p.o.; atenolol (25 mg kg−1 day−1) p.o.; DPSPX (90 μg kg−1 h−1) i.p. + losartan (15 mg kg−1 day−1) p.o.; DPSPX (90 μg kg−1 h−1) i.p. + atenolol (25 mg kg−1 day−1) p.o. Blood pressure was measured by the ‘tail-cuff’ method in conscious animals. Fragments of the mesenteric and tail arteries were processed for morphological study and the mean diameter of the vascular smooth muscle cells was determined. 4 DPSPX increased blood pressure. Losartan and atenolol prevented this rise but had no effect on blood pressure of control rats. DPSPX-treated groups showed hypertrophy of the vascular smooth muscle cells and proliferation of subintimal cells. Losartan but not atenolol prevented these changes. Losartan had no effect on the vascular morphology of control rats, while treatment with atenolol for 4 weeks induced hypertrophy of the vascular smooth muscle cells. 5 Both losartan and atenolol counteract the development of DPSPX-induced hypertension but only losartan prevents the alterations in vascular morphology.

Notizen: 1 The characteristics of semicarbazide-sensitive amine oxidase (SSAO) are reviewed and the unknown physiological or pathological role of this enzyme emphasized. 2 The various mechanisms of action proposed for the vasodilator drug hydralazine are considered. In particular, the inhibitory action on various enzymes, related or not to cardiovascular function, are discussed. 3 Studies linking inhibition of SSAO to hydralazine hypotension are reviewed and a general hypothesis relating both actions is presented. The hypothesis postulates that (a) vascular SSAO is involved in the regulation of vascular tone, and (b) hydralazine vasodilation is the consequence of vascular SSAO inhibition. 4 Evidence supporting these postulates is presented and vascular SSAO inhibition is proposed as a novel mechanism of vasodilation.

Notizen: 1 The regulation by angiotensin II (Ang II) formed locally on nerve-stimulated purinergic and adrenergic components of double-peaked vasoconstrictions in the canine splenic artery and Ang II receptor subtypes involved were investigated. 2 The perfusion of the precursor angiotensin I (Ang I, 0.1–1 nm) did not affect the vasoconstrictor responses to noradrenaline (NA, 0.03–1 nmol) and adenosine 5′-triphosphate (ATP, 0.03–1 μmol). The second component vasoconstrictor response to nerve stimulation was dose dependently potentiated by Ang I (0.1–1 nm). The first peaked constriction was slightly, but insignificantly increased. The potentiating effects of Ang I were abolished by KRH-594 (10 nm), a selective AT1 receptor antagonist, but not by PD 123319 (1–10 nm), an AT2 receptor antagonist. KRH-594 (10 nm) or PD 123319 (10 nm) never affected the vasoconstrictions to either NA or ATP. 3 The treatment with KRH-594 (1–10 nm) produced a greater inhibition on the second peaked response than the first one, although both of them were dose dependently inhibited. PD 123319 (1–10 nm) did not affect the vasoconstrictor responses induced by nerve stimulation. 4 Inhibition of angiotensin-converting enzyme with 10 nm enalaprilat reduced the second peaked response, having no significant inhibition on the first peaked response. A higher dose of enalaprilat (100 nm) produced a greater inhibition of the second peak than the first one. It reduced the second peak by approximately 65%, while the first peak was decreased approximately 35%. After treatment with enalaprilat, Ang I (1 nm) failed to enhance the neuronal vascular response. Enalaprilat at doses used did not affect the vasoconstrictions to either NA or ATP. 5 The present results indicate that endogenously generated Ang II may produce a more marked potentiation of adrenergic transmission than purinergic transmission via activation of prejunctional AT1 receptors.

Notizen: 1 The aim of this study was to investigate the co-localization of histamine and dopamine-β-hydroxylase in the superior cervical ganglion of guinea-pig and release of histamine from cardiac sympathetic terminals in guinea-pig isolated atrium. 2 Histidine decarboxylase (a histamine-synthesizing enzyme) mRNA signals were detected in the neurones of superior cervical ganglion of guinea-pig by in situ hybridization. The results of double-labelled immunofluorescence further confirmed the co-localization of histamine and dopamine-β-hydroxylase in the large principle neurons and small intensely fluorescent cells in the superior cervical ganglion. The immunoreactivities of both histamine and dopamine-β-hydroxylase were significantly attenuated after 6-hydroxydopamine-induced lesion of sympathetic nerves. 3 The refractory electrical field stimulation caused the release of histamine from cardiac sympathetic terminals of guinea-pig isolated atria (112.14 ± 40.34 ng ml−1), which was significantly attenuated to 35 ± 15.57 ng ml−1 by reserpine pretreatment. Following administering compound 48/80, a mast cell degranulator, electrical field stimulation induced a dramatic increase of endogenous histamine release from isolated atria (303.57 ±72.93 ng ml−1). When compound 48/80 was added to the reserpine-treated atria, the release of histamine induced by field stimulation was decreased to 207.14 ± 76.39 ng ml−1. 4 These results provide novel evidence that histamine co-exists with noradrenaline in sympathetic nerves and might act as a neurotransmitter to modulate sympathetic neurotransmission.

Notizen: 1 The aim of the current study was to investigate in HT-29 human colon cancer cell line, the existence of functional receptors for the signalling molecules, noradrenaline (NA), prostaglandin E2 (PGE2), and adenosine-5′-triphosphate (ATP).2 We utilized microphysiometry, which monitors on-line extracellular acidification rate (ECAR) as a measure of cellular metabolic activity, and how this variable is altered by signalling molecules.3 Challenge with NA (5.9 μm) resulted in an increase in ECAR by approximately 24% of basal.4 PGE2 (0.0284, 0.284 and 2.84 μm) hardly affected ECAR.5 ATP (100 μm) elicited a biphasic effect on ECAR (increase and decrease in ECAR by about 58 and 10% of basal, respectively).6 HT-29 cells were shown to express COX-2 by immunocytochemistry.7 These data suggest the presence of functional receptors for NA and ATP, but not for PGE2 in HT-29 human colon cancer cell line.

Notizen: 1 Cardiac vagal outflow is the major factor determining the magnitude of heart rate (HR) variability analysed by traditional time and frequency domain methods. New analysis techniques, such as fractal and complexity methods, have been developed to probe non-linear features in HR behaviour that may not be detectable by traditional methods.2 We investigated the effects of vagal blockade (glycopyrrolate i.v. 5 μg kg−1 h−1 for 2 h, n = 8 vs. unmedicated control group, n = 8) and various breathing patterns (n = 12) on two non-linear measures of HR variability – detrended fluctuation analysis (DFA) and approximate entropy (ApEn) – in healthy male volunteers.3 Glycopyrrolate decreased the mean (±SD) ApEn from 1.46 ± 0.18 to 0.85 ± 0.24 (P = 0.001 in comparison with the control group), and increased the short-term (α1) and intermediate-term (α2) fractal scaling exponents of DFA, α1 from 0.96 ± 0.19 to 1.43 ± 0.29 (P = 0.003) and α2 from 1.13 ± 0.10 to 1.34 ± 0.14 (P 〈 0.001).4 Decrease in fixed respiration rate from 15 to 6 breaths min−1 increased α1 from 0.83 ± 0.25 to 1.18 ± 0.27 (P 〈 0.001), but decreased α2 from 0.88 ± 0.09 to 0.45 ± 0.17 (P 〈 0.001) and ApEn from 1.26 ± 0.12 to 1.10 ± 0.14 (P = 0.028). Rapid breathing (24 min−1) had no influence on these non-linear measures of HR variability. Hyperventilation (15 min−1, tidal volume increased voluntarily by 0.5 l) decreased α1 from 0.83 ± 0.25 to 0.66 ± 0.28 (P = 0.002) but did not affect α2 or ApEn.5 To conclude, vagal blockade alters the fractal scaling properties of R-R intervals (α1, α2) and reduces the complexity (ApEn) of HR behaviour. Both the fractal and complexity measures of HR variability can also be influenced by changes in the breathing pattern.

Notizen: 1 Inflammatory bowel disease (IBD) is a condition that involves proinflammatory cytokines such as interleukins 1β and 6 (ILs). In this disease, it has been shown that an abnormal microcirculatory system is implicated. 2 Therefore, the effects of in vivo treatment for three days with interleukins 1β and 6 were investigated on rat isolated mesenteric vascular bed (MVB). 3 A significant concentration-dependent increase in vascular response to noradrenaline (NA) was found, with a significant difference in Emax between control (93.01 ± 16.78 mmHg) and treated preparations (137.91 ± 5.20 mmHg). Endothelin-1(ET-1) induced a significantly greater increase of perfusion pressure in treated rats in comparison with control rats at the highest concentration used (0.1 μm). 4 The concentration-dependent decrease of perfusion pressure induced by acetylcholine (ACh) in MVB precontracted with NA was significantly reduced in specimens from treated rats in comparison with control rats, with a significant difference in Emax between control and treated preparations. 5 Perivascular nerve stimulation (PNS) evoked contractions with no difference between treatments. Similarly, no difference in relaxant effect was found after PNS in specimens precontracted with NA, in the presence of guanethidine. 6 These findings indicate that the precocious inflammation acts only at postsynaptic level, facilitating vascular contraction. These data seem to support the hypothesis that vascular dysfunction caused by overproduction of ILs may contribute, among other immunological factors, to vasculitis in IBD that leads to intestinal ischaemia through vasoconstriction.

Notizen: 1 The present study was designed to analyse the possible involvement of V1- and V2-receptors in vasopressin (AVP)-induced facilitation of the sympathetic nervous system. Furthermore, we aimed to determine whether the site of facilitation by AVP is located pre- or postsynaptically. 2 Electrical field stimulation (EFS) was applied on the rat mesteric artery to activate the sympathetic nervous system. In addition, we evaluated the direct vascular effects of AVP. The postsynaptic effect of AVP on the sympathetic nervous system was investigated by exposing the vessels to exogenous noradrenaline. These experiments were performed in the absence or presence of selective V1 and V2 receptor antagonists SR 49059 and SR 121463, respectively. Desmopressin was applied as a selective V2 agonist. 3 The direct vasoconstrictor effect of AVP was antagonized by SR 49059 and not by SR 121463. Desmopressin neither showed any direct vasoconstrictor effect nor produced vasodilatation after a precontraction induced by noradrenaline (10 μm). The EFS-induced rise in vascular tone could be increased by a sub-pressor concentration of AVP. This fascilitation could be antagonized by SR 49059, but not by SR 121463. Desmopressin did not influence the increase in vascular tone during EFS. Vasoconstriction induced by exogenous noradrenaline could be facilitated by a sub-pressor concentration of AVP and this selective postsynaptic effect could be antagonized by V1-receptor blockade. 4 In conclusion, the AVP-induced facilitation of the sympathetic nervous system is completely V1-receptor dependent and at least partly postsynaptically mediated.

Notizen: 〈list xml:id="l1" style="custom"〉1To validate the relationship between the dopaminergic, opioidergic and cholinergic nervous systems, we evaluated the effect of domperidone, a dopamine (D2) antagonist, on the opioid system in myenteric plexus-longitudinal muscle preparation isolated from the guinea-pig ileum.2One micromolar of domperidone did not affect the 0.1 Hz-evoked (duration 0.5 ms, maximum intensity) twitch response, but concentration dependently inhibited the twitch response between concentrations of 2 and 20 μm, and the inhibition was maximum after 20–30 min at the highest concentration used (20 μm).3 Acetylcholine-evoked contraction on basal tension was also not inhibited by 1 μm domperidone, but the contraction was concentration dependently inhibited at concentrations of 10–100 μm in a non-competitive manner.4One micromolar of domperidone, however, increased post-tetanic twitch inhibition, an indicator of the release of endogenous opioids. This increase was completely antagonized by 1 μm naloxone.5Twitch inhibition induced by dynorphin 1–13 (0.1–10 nm) was not affected by 1 μm domperidone, but increased the maximum twitch inhibition caused by morphine (0.1–1 μm).6These results might reflect the existence of an interaction between the dopaminergic and opioidergic system without the inhibition of the cholinergic system. Dopamine antagonists increased opioid action, an action that may depend more on the increased release of endogenous opioids than on supersensitivity of the opioid receptor.

Notizen: 1 The present study compared the effect of the administration of tachykinin NK1- and NK2-receptor antagonists alone and in combination on exogenous and endogenous tachykinin-induced contractions using three different guinea-pig airway preparations: isolated bronchus, isolated perfused lung and in vivo. 2 In the isolated bronchi, the tachykinin NK1-receptor antagonist CP 99994 (0.01–1 μm)produced concentration-dependent inhibition of contractions induced the tachykinin NK1-receptor agonists substance P (SP) and [Met-OMe11] SP ([Met-OMe11]SP), whereas the tachykinin NK2-receptor antagonist SR 48968 (0.1 μm) had no effect. SR 48968 (0.001–0.01 μm)concentration-dependently inhibited contractions induced by the tachykinin NK2-receptor agonists neurokinin A (NKA) and [β-Ala8]-neurokinin A (4–10) ([βAla8]-NKA) whereas CP 99994 (0.1 μm) did not inhibit the contractions. The contractile activity of capsaicin, an agent that releases endogenous tachykinins from sensory C-fibres, was inhibited in a concentration dependent manner by SR 48968 (0.001–0.03 μm) but not by CP 99994 (0.1 μm). Combination of CP 99994 and SR 48968 caused increased inhibitory effects on the concentration–response curves to SP, [Met-OMe11]SP, NKA, [β-Ala8]-NKA and capsaicin. 3 In isolated perfused lungs, SR 48968 concentration (0.01–10 μm) dependently inhibited NKA-, [β-Ala8]-NKA- and capsaicin-induced bronchoconstriction whereas CP 99994 (30 μm) had no effect on SP-, NKA-, [β-Ala8]-NKA- and capsaicin-induced bronchoconstriction. Combination of inactive concentrations of CP 99994 and SR 48968 produced an increased inhibitory effect on all previous stimuli-induced bronchoconstriction. 4 In in vivo guinea-pig studies, intravenous and oral pretreatment with SR 48968 (0.01–1 mg kg−1 i.v. and 0.1–3 mg kg−1 p.o., respectively), but not with CP 99994 (1 mg kg−1 i.v. and 0.3–30 mg kg−1 p.o., respectively), produced a dose-dependent inhibition of the bronchoconstrictor responses induced by NKA, [β-Ala8]-NKA and capsaicin. CP 99994 intravenously (0.3 mg kg−1) and orally (3–10 mg kg−1) inhibited SP-induced bronchoconstriction only. Intravenous and oral low dose combinations of CP 99994 and SR 48968 produced an increased inhibition of SP-, NKA-, [β-Ala8]-NKA- and capsaicin-induced bronchoconstriction, respectively. 5 The present data indicate that combined tachykinin NK1- and NK2-receptor antagonist treatment compared with single antagonist treatment, using CP 99994 and SR 48968, produced an augmented blockade of tachykinin NK1-, NK2- and capsaicin-mediated contractions in guinea pig airways. These findings support the hypothesis that a dual NK1- and NK2-receptor antagonist may provide an advantage over single activity tachykinin NK1- or NK2-receptor antagonists in pulmonary obstructive diseases.

Notizen: 1 One reason why rabbit jejunum is suitable for studying the mechanisms underlying the actions of the various neurotransmitters and their interactions is its spontaneous motility. The main regulator of spontaneous motility is the cholinergic system. How the cholinergic system regulates the spontaneous activity in the rabbit jejunum and how it affects the inhibitory action of α- and β-adrenoceptor agonists remains unclear. 2 We studied the influence of the cholinergic system and apamin-sensitive Ca2+-activated K+ channels on spontaneous contractions in the rabbit jejunum and on the inhibitory effects ofα1- and β-adrenoceptor agonists. 3 In naïve tissues, atropine (ATR, 7.4 × 10−8 m) and tetrodotoxin (8 × 10−8 m) almost completely inhibited – to a similar extent – the amplitude of spontaneous activity. Despite the presence of ATR or TDX, tissue contraction gradually recovered to about 50% of the baseline amplitude within 5–10 min. When ATR or TDX, respectively, were added to the TDX- or ATR-treated tissues, the recovered activity decreased weakly but significantly. After washout and a 45-min rest the contraction amplitude returned to baseline values. A further exposure to ATR or TDX reduced the contraction to a level significantly lower than the one obtained after TDX or ATR added 5 min after ATR or TDX, respectively. In preparations prestimulated for 10 min with acetylcholine (ACh), ATR abolished the TDX-resistant recovered spontaneous activity. 4 Adrenaline (ADR, 0.5–5 × 10−7 m) and phenylephrine (PHE, 1–10 × 10−7 m) inhibited tissue motility in naïve and in ATR- and in TDX-exposed preparations. But whereas in naïve preparations the α1-adrenoceptor antagonists completely antagonized inhibition induced by both drugs, in ATR- and TDX-exposed tissues they did so only partially for ADR. Agonist-induced inhibition had a rapid onset but rapidly faded; pendular movements took significantly longer to recover in ATR- and TDX-treated tissues than in naïve tissues. In tissues exposed for 2 min to ADR (0.5–5 × 10−7 m) or PHE (1–10 × 10−7 m), washout or addition of α1-adrenoceptor antagonists caused an immediate short-lasting increase in contraction amplitude. 5 Apamin (APAM, 5 × 10−9 m) caused a rapid and persistent increase in the amplitude of contractions. It also blocked the inhibitory responses to ADR and PHE, and removed washout-induced contractions. The APAM-induced increase in the contraction amplitude correlated with the increase obtained by washing out ADR or PHE. 6 Isoprenaline (at concentrations up to 2.8 × 10−7 m) produced no inhibitory response in naïve tissues, but it invariably blocked (at a concentration of 0.7 × 10−7 m) the recovered spontaneous activity (and sometimes depressed muscletone) in tissues exposed to ATR or TDX. Neither propranolol (3.4 × 10−7 m) nor APAM (5 × 10−9 m) counteracted these inhibitory effects. 7 These results indicate that spontaneous motility in the rabbit jejunum is predominantly mediated by neuronal release of ACh and by some other unidentified neuronal activity. Released ACh inhibits myogenic activity and strongly antagonizes β-adrenoceptor-induced APAM-insensitive inhibition but leaves α1 agonist-induced APAM-sensitive inhibition unchanged.

Notizen: 1 This study used contractility methods with the portal veins of 5- and 14-week-old Wistar–Kyoto normotensive rats (WKY) and spontaneously hypertensive rats (SHRs). The SHRs are prehypertensive at 5 weeks. 2 The first part of our study was to determine whether the responsiveness to isoprenaline and forskolin was altered in the maturation of portal veins from normo- and prehypertensive rats. The responses to forskolin were similar on the portal veins of 5- and 14-week-old WKY and SHRs. 3 The sensitivity and maximum responses to isoprenaline were similar on portal veins of 5- and 14-week-old WKY. The sensitivity and maximum responses to isoprenaline were lower on the portal veins of 5-week-old SHRs (pD2 = 8.25, maximum = 85%) than age-matched WKY (pD2 = 8.79, maximum = 96%); these differences are not caused by hypertension. At 14 weeks, the sensitivity was similar (WKY pD2 = 8.74, SHR pD2 = 8.65) but the maximum responses to isoprenaline were lower on the portal veins SHRs (77%) than WKY (97%). Thus, the sensitivity to isoprenaline increases with the development of hypertension in the SHR portal vein. 4 The second part of the study was to determine whether the affinity for isoprenaline at β2-adrenoceptors and the fractional β2-adrenoceptor occupancy–response relationships on the portal vein were altered in maturation from normo- and pre-hypertensive rats. The effects of bromoacetylalprenololmenthane (BAAM), an irreversible β-adrenoceptor blocker, on the isoprenaline responses of 5- and 14-week-old WKY and SHRs were studied. Maturation of the WKY portal vein between 5 and 14 weeks was associated with a loss of affinity for isoprenaline (from pKA of 7.13 to 7.87), and increase in β2-adrenoceptor reserve (from 72 to 92% at the 95% response). There were similar affinity and reserve findings in the maturation of the SHR portal vein. Thus, there are major changes in β2-adrenoceptor structure and reserve in maturation on the portal vein that are irrespective of the development of hypertension.

Notizen: 1 It is generally recognized that the vasodilator hydralazine produces hypotension accompanied by baroreflex-mediated tachycardia. In some experimental conditions, however, the accompanying heart rate change is bradycardia, a paradoxical response which has not been satisfactorily explained. The present study examined the possibility of hydralazine-induced bradycardia being mediated by vagal or sympathetic afferents activated by changes in left ventricular pressure. 2 Systolic blood pressure and heart rate responses to hydralazine were recorded in conscious normotensive intact rats by a tail cuff method and compared with responses in animals subjected to previous sino-aortic deafferentation (SAD) to remove the influence of the arterial baroreflex. Responses were also obtained after blockade of myocardial afferent vagal C-fibres with urethane, of efferent vagal impulses to the heart with methylatropine, of positive inotropic effects of hydralazine with atenolol, and of prostanoid sensitization of myocardial nerve fibres with indomethacin. 3 Hydralazine produced hypotension and tachycardia in intact rats, and hypotension and bradycardia in SAD animals. In intact rats, this pattern was not affected by any of the pretreatments, while in SAD rats, all pretreatments reversed the bradycardia to hydralazine. 4 The present results indicate that suppression of the arterial baroreflex by SAD propitiates the appearance of a bradycardiac response to hydralazine. This reaction probably results from activation of a vagal cardiodepressant reflex originating in the heart, as suggested by its blockade by drugs acting at various sites along the reflex arch.

Notizen: 1 In the present study, we investigated the effect of chronic ethanol administration on nitric oxide (NO)-mediated responses in rat isolated trachea preparation. 2 Ethanol was given to rats in a modified liquid diet for 21 days. Isolated tracheal rings were then used to obtain responses to electrical field stimulation (EFS) after precontraction with 100 μm histamine. The parameters of field stimulation were as follows: supramaximal voltage of 50 V, 0.5 ms duration, 10-s train; 0.5, 1, 3, 5, 10, 20, 30 and 50 Hz at 2-min intervals. The effects of l-and d-arginine (10−6 m) on the responses to field stimulation (10–20 Hz) were studied. In other experiments, we tested the effects of Nω-nitro-l-arginine methyl ester (l-NAME, 10−6–10−5 m) and SIN-1 (10−6–10−5 m) on the responses to field stimulation. 3 Electrical field stimulation induced relaxation responses in the tracheal rings precontracted with histamine from control- and ethanol-treated rats. The relaxation responses induced by EFS were significantly reduced in the tracheal rings precontracted with histamine from ethanol dependent group. The responses induced by EFS in both groups were completely abolished by tetrodotoxin (1 μm), but unaffected by hexamethonium (1 μm). Incubation with d-arginine did not cause statistically significant increases in relaxation responses to EFS in both groups. l-Arginine (10−6 m) caused statistically significant increases in relaxation responses to EFS in control rats, but not in ethanol dependent rats. Incubation with l-NAME (10−6–10−5 m) caused statistically significant inhibition of the relaxation responses to EFS in both groups. SIN-1 (10−6–10−5 m) induced significantly increase in relaxation responses to EFS in both groups. 4 Our results suggest that the possible mechanism responsible for inhibition of tracheal inhibitory responses to EFS in ethanol-dependent rats may be a reduction in production of NO and in the uptake of l-arginine.

Notizen: Abstract Optical single transporter recording (OSTR) is an emerging technique for the fluorescence microscopic measurement of transport kinetics in membrane patches. Membranes are attached to transparent microarrays of cylindrical test compartments (TCs) ~0.1-100 mum in diameter and ~10-100 mum in depth. Transport across membrane patches that may contain single transporters or transporter populations is recorded by confocal microscopy. By these means transport of proteins through single nuclear pore complexes has been recorded at rates of 〈1 translocation/s. In addition to the high sensitivity in terms of measurable transport rates OSTR features unprecedented spatial selectivity and parallel processing. This article reviews the conceptual basis of OSTR and its realization. Applications to nuclear transport are summarized. The further development of OSTR is discussed and its extension to a diversity of transporters, including translocases and ATP-binding cassette (ABC) pumps, projected.