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  • Articles  (196)
  • Hymenoptera  (70)
  • photosynthesis  (68)
  • lipid peroxidation  (58)
  • Springer  (196)
  • American Chemical Society
  • American Institute of Physics (AIP)
  • Blackwell Publishing Ltd
  • 1990-1994  (196)
  • 1992  (112)
  • 1991  (84)
  • Biology  (196)
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  • Articles  (196)
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  • Springer  (196)
  • American Chemical Society
  • American Institute of Physics (AIP)
  • Blackwell Publishing Ltd
  • Wiley-Blackwell  (5)
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  • 1990-1994  (196)
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  • 1
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Braconidae ; Lepidoptera ; Pieridae ; cabbage ; tritrophic interactions ; semiochemicals ; host-habitat location ; foraging behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Upon initiating a research project on the role of volatile infochemicals in the tritrophic system Cotesia (= Apanteles) glomerata (L.)-Pieris brassicae (L.)-cabbage, a bioassay was developed to investigate the response of C. glomerata. The bioassay should be effective in terms of high responsiveness and minimum variability and constructed through a comparative approach. Twenty seven treatments, organized in a factorial randomized block design, compared the effect of three bioassay set-ups (glasshouse flight chamber, wind-tunnel and Y-tube olfactometer), three parasitoid age groups (1–2, 4–5 and 8–9 days old females), three pre-treatment experiences (naive, damage experienced and oviposition experienced wasps) and the day-to-day effect on response of C. glomerata to clean cabbage (CC) and planthost complex (PHC) in a dual choice test. The best results with regard to the strength and consistency of response to the PHC were obtained in the glasshouse flight-chamber by 4–5 days old female wasps with either damage or oviposition experience (94 and 90%, respectively). It is therefore recommended as a suitable bioassay for studying the role of volatile infochemicals in host-habitat location by C. glomerata. A day-to-day variation in response was found in the glasshouse and wind-tunnel. It was correlated with the direction of change in barometric pressure within the time period of the experiment, showing that steadily increasing atmospheric pressure yields a significantly higher response than steadily decreasing or fluctuating barometric flux. To control for the day effect it is suggested to conduct further experiments in a block design, having day as a block. Several aspects of the infochemical ecology of C. glomerata are discussed.
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  • 2
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    Entomologia experimentalis et applicata 63 (1992), S. 101-102 
    ISSN: 1570-7458
    Keywords: Cnaphalocrosis medinalis ; rice leaffolder ; Oryza sativa ; rice ; photosynthesis ; transpiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 3
    ISSN: 1570-7458
    Keywords: Eurytoma amygdali ; Hymenoptera ; almond pest ; sex pheromone ; population monitoring ; chemical control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During the four years 1986–1989 we monitored an adult male population of the almond seed wasp, Eurytoma amygdali Enderlein, in the region of Thessaloniki, using sex pheromone traps, each containing 25 living virgin females. The emergence of adults from infested almonds of the previous year was also monitored in cages in the field, as well as the progress of fruit infestation and egg hatching in the almonds of the Retsou variety. Finally, two chemical control experiments were done against the newly hatched larvae to determine the optimal time for chemical control. During the 4-year study the start of adult captures occurred between April 13 and April 24, and the maximum between April 17 and May 11. In most cases, adult emergence from infested almonds in field cages began with a delay of 2 to 14 days in relation to the start of trap captures, and was affected by factors such as the date of cage installation. In all years most eggs hatched within 12–18 days of oviposition. A treatment with phosphamidon at a concentration of 0.06%, applied to tree branches of the Retsou variety with a hand-sprayer on May 23, 1987, when 54.1% of the eggs had hatched, was very effective (final infestation in July was 4.7% as compared to 89.1% of the untreated control). Three treatments with phosphamidon 0.06%, applied each on different tree branches, on May 14, 20, and 25, 1988, when the percentage of egg hatching was 7.8%, 65.3%, and 93.5%, respectively, were all very effective (final infestation in July was 1.6%, 3.6%, and 4.2% respectively, as compared to 93.6% of the untreated control). However, the presence of mines in the perisperm and outer endosperm, caused by the feeding larvae before they were killed, was more conspicuous in the almonds treated when the percentage of egg hatching was high. Our data suggest that the almond seed wasp can be effectively controlled with a single treatment of a systemic insecticide against the newly hatched larvae, preferably applied when the percentage of egg hatching is low to medium (between 10% and 50%). In the area of Thessaloniki, during 1986–89, 10% and 50% of egg hatching occurred about 21 and 27 days respectively after the beginning of male adult captures in pheromone traps.
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  • 4
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Diprionidae ; chiral compounds ; behavioural activity ; attractant ; inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract All eight optical isomers of 3,7-dimethyl-2-pentadecanyl acetate (diprionyl acetate), of high optical purity (〉 97.4%), were tested for a behavioural activity on male pine sawflies, Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae), in northern Europe. Males were strongly attracted to (2S,3S,7S)-diprionyl acetate. Addition of more than 0.1% of the (2S,3R,7R)-isomer reduced the catch and above 2% the attraction was completely inhibited. Contrary to what has been reported for North American and Japanese populations, so significant synergistic effect of small amounts of the (2S,3R,7R)-isomer could be demonstrated. The effects of addition of the other six optical isomers alone or in combinations, were also studied, but none was found to be a synergist. The (2S,3R,7S)-isomer had a weak inhibitory effect, and completely inhibited the attraction to the (2S,3S,7S)-isomer when applied in about equal amounts as the attractant. In some cases a reduction in catch was noted when other isomers were tested, but this could be attributed to the very small amounts of the inhibitory (2S,3R,7R)-isomer present in these isomers.
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  • 5
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    Entomologia experimentalis et applicata 64 (1992), S. 117-124 
    ISSN: 1570-7458
    Keywords: Learning ; specialist ; generalist ; parasitoid ; Leptopilina ; foraging behaviour ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effects of learning in two microhabitat specialists, Leptopilina boulardia Barbotin et al. and L. fimbriata Kieffer were compared to previous and new results of learning in the microhabitat generalist L. heterotoma Thomson. Females were given one or more oviposition experiences on hosts in different types of substrate. In all species oviposition experience affected the choice for a substrate, although this effect of learning was considerably less in L. fimbriata compared to the other two species. Patch times, known to be highly determined by experience in the generalist L. heterotoma, were much less flexible in the specialists. L. boulardi and L. fimbriata have fixed patch times on their natural substrate and have variable patch times on other substrates only. In all three species one oviposition affected the choice for a substrate. Additional ovipositions showed no different effect. An accumulative effect of the number of ovipositions on patch times was found in L. heterotoma only. Retention of the learning effect was only studied in L. boulardi, and was shown to be similar to that reported for L. heterotoma, i.e. two to three days. Although learning was found in both the generalist and the specialist species studied, it seems to affect their foraging behaviour differently.
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  • 6
    ISSN: 1572-8889
    Keywords: Seed chalcid ; Bruchophagus spp. ; Hymenoptera ; Eurytomidae ; Chalcidae ; olfactory behavior ; polarization vision ; insect behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When the alfalfa [Bruchophagus roddi (Gussakovsky)], clover [Bruchophagus gibbus (Boheman)], and trefoil seed chaldds (TSC) [Bruchophagus platypterus (Walker)] were exposed to yellow, white, green, and purple painted polyethylene vials perforated by four small holes, only the latter species had a color preference, and that was for yellow, the color of its host flower. When TSC were exposed to green and yellow targets 5 h after sunrise, they preferred yellow targets but not 1 h after sunrise. The possibility of a circadian response was eliminated because different sequences of light-dark regimes prior to the test did not change the results. When TSC were exposed only to yellow targets, half of which had trefoil flowers hidden within, females preferred targets with flowers. When an identical test was conducted but with green instead of yellow targets, the preference for targets with flowers disappeared. In a four-choice test, TSC preferred yellow targets with or without flowers to green targets with or without flowers. Thus, TSC displayed an olfactory response only when the color yellow was present. In unfiltered skylight females preferred baited targets when the test began 3 h before or 1 h after solar noon but not 4 h before or 2 h after solar noon. Chalcids did display an olfactory preference 4 h before solar noon when a Polaroid filter was used to filter skylight and provide an east-west but not a north-south E-vector. When Helmholtz coils were used to apply a magnetic field that canceled or changed the direction of the earth's magnetic field, olfactory preference disappeared because the applied magnetic field changed TSC perception of the E-vector. In effect, TSC must perceive yellow in the presence of an east-west E-vector to display an olfactory preference to a choice of odors. We believe this is the first report that the E-vector of celestial light can influence olfactory and visual behavior of an insect.
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  • 7
    ISSN: 1572-8889
    Keywords: postemergence learning ; cross-induction ; foraging behavior ; Cotesia congregata (Say) ; Braconidae ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Postemergence experience with one of six plant species, in the presence of the host larva, modified the searching response of reproductively mature females of Cotesia congregata(Say) to these plants in at least one of three ways: (1) an increased response to the plant experienced at emergence, (2) an increased response to other plants, or (3) an inhibited response to other plants. Landing and searching responses were differentially affected by postemergence experience. For example, postemergence experience with tobacco (a common plant) in the presence of the host larva induced a landing preference for this plant over parsley (a novel plant) but did not affect searching responses to either plant, whereas experience with parsley and the host larva induced an increased searching response to parsley but a landing preference for tobacco. Differential effects of postemergence experience may reflect the type of stimuli involved in searching or landing and may have adaptive significance.
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  • 8
    ISSN: 1572-8889
    Keywords: Hymenoptera ; Braconidae ; Opius dissitus ; Diptera ; Agromyzidae ; Liriomyza sativae ; semiochemical ; volatile ; foraging behavior ; adult experience ; learning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Oviposition-experienced females of Opius dissitus Muesebeck, a braconid parasitoid of Liriomyza sativaeBlanchard, preferentially landed on leafminer-infested rather than uninfested lima bean (Phaseolus lunatus L.) plants in a flight tunnel assay. Both naive and oviposition-experiencedparasitoids responded strongly to odors of infested lima bean plants in a four-arm olfactometer in comparison with odors of uninfested plants, suggesting that volatile semiochemicals are used in host location. Parasitoids with an oviposition experience on lima bean (“lima-experienced”) spent significantly more time in the infested odor than naive individuals, however, eggplant-experienced wasps did not spend significantly more time in the infested odor field than naive wasps. When parasitoids reared on leafminers in lima bean were provided a choice between the odor of infested lima bean and the odor of infested eggplant or cotton, naive and lima-experienced wasps preferred infested lima odor. An oviposition experience on the other plant species resulted in a dramatic shift in preference. It was concluded that the experience effect was due, at least in part, to associative learning, as has been reported for other parasitoids. The parasitoids may perceive unconditioned stimuli during host contact and oviposition on an infested leaf and may associate those stimuli with volatile semiochemicals emanating from the leaf or host. Subsequently, the volatiles associated with the presence of hosts are used in directing the search for hosts.
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  • 9
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    Entomologia experimentalis et applicata 63 (1992), S. 229-236 
    ISSN: 1570-7458
    Keywords: Azinphosmethyl ; time response tests ; resistance ; Trioxys pallidus ; biological control ; genetic improvement ; Hymenoptera ; Aphidiidae ; gene amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Homogeneity in azinphosmethyl resistance was assessed in males of a laboratory-selected (Select-17) and susceptible (Yolo) colony of Trioxys pallidus Haliday (Hymenoptera: Aphidiidae) using a time response assay. No evidence of heterogeneity within the two colonies was found. Reciprocal crosses between the Yolo and the Select-19 (the Select-17 colony following two additional selections) colonies resulted in F1 females that exhibited a semidominant response to azinphosmethyl with a dominance value (D) of 0.32, as well as no evidence of maternal effects or sex linkage. Responses of F2 progeny to azinphosmethyl suggest that more than one gene may be involved because no inflection was observed in the time response lines of F2 males. Additional research is required to fully elucidate the mode of inheritance.
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  • 10
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    Cellular and molecular life sciences 48 (1992), S. 1030-1041 
    ISSN: 1420-9071
    Keywords: Ultraweak luminescence ; photon emission ; biophoton emission ; mitogenetic radiation ; lipid peroxidation ; yeast ; bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The results of recent work on the photon emission from three yeasts and a bacterium is presented. Both visible region and ultraviolet photon emission is observed; however, no luminescence is observed in the absence of oxygen. The visible region emission is attributed to excited carbonyl groups and excited singlet oxygen dimers formed during the decomposition of lipid hydroperoxides. Possible sources of the ultraviolet photon emission are also examined. The use of microorganisms in the study of ultraweak photon emission and its relation to oxidative, temperature and chemical stress is reviewed and the applications and (or) functions of this photon emission are also discussed.
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  • 11
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    Cellular and molecular life sciences 48 (1992), S. 414-416 
    ISSN: 1420-9071
    Keywords: Solitary bees ; lipid polymer ; silk ; CP/MAS13CNMR ; Hymenoptera ; Colletidae ; Hylaeus bisinuatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The nest cell lining ofHylaeus bisinuatus (Hymenoptera: Colletidae) was shown by high-resolution solidstate [13C]NMR to be composed of lipid polymer and protein. The lipid polymer was shown by reduction and subsequent GC/MS analysis to be comprised of ω-hydroxy fatty acids (C20, C22, C24 and C26) and fatty alcohols (C16 to C30). The protein portion of the lining had a silk-like amino acid composition.
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  • 12
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    BioMetals 5 (1992), S. 149-156 
    ISSN: 1572-8773
    Keywords: Hg2+ toxicity ; cyanobacterium ; Nostoc calcicola ; growth ; photopigments ; nucleic acids ; photosynthesis ; membrane integrity ; nutrient uptake ; enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Toxicological responses of the filamentous N2-fixing cyanobacteriumNostoc calcicola Bréb. towards Hg2+ were studied to enumerate the decisive lethal events. In low-dose, long-term experiments (0.05–0.25 μm Hg2+, 10 days), photoautotrophic growth was severely inhibited with concurrent loss of photosynthetic pigments (phycocyanin〉chlorophyll α〉carotenoids) and nucleic acids. The termination of growth after a day 4 exposure to 0.25 μm Hg2+ has been attributed to the complete inhibition ofin vivo photosynthetic activity in the cyanobacterium (O2 evolution〉14CO2 incorporation). The elevated Hg2+ concentrations irreversibly damaged the cell membrance as observed under light microscopy, and as indicated by the leakage of intracellular electrolytes and phycocyanin. In high-dose, short-term experiments (0.5–20.0 μm Hg2+, up to 6 h), thein vivo activities of selected enzymes (glutamine synthetase 〉 nitrate reductase 〉 nitrogenase) were less inhibited by Hg2+ than the uptake of nutrient ions (NH 4 + 〉NO 3 − 〉PO 4 3− ).
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  • 13
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    Journal of comparative physiology 171 (1992), S. 171-181 
    ISSN: 1432-1351
    Keywords: Colour vision ; Flower colours ; Evolution ; Hymenoptera ; Pollination ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The evolutionary tuning between floral colouration and the colour vision of flower-visiting Hymenoptera is quantified by evaluating the informational transfer from the signalling flower to the perceiving pollinator. The analysis of 180 spectral reflection spectra of angiosperm blossoms reveals that sharp steps occur precisely at those wavelengths where the pollinators are most sensitive to spectral differences. Straight-forward model calculations determine the optimal set of 3 spectral photoreceptor types for discrimination of floral colour signals on the basis of perceptual difference values. The results show good agreement with the sets of photoreceptors characterized electrophysiologically in 40 species of Hymenoptera.
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  • 14
    ISSN: 1423-0445
    Keywords: chemical defence ; tri-trophic interactions ; alkaloids ; Homoptera ; Aphis craccivora ; Icerya spp. ; Lepidosaphes ulmi ; Planococcus citri ; Coleoptera ; Coccinellidae ; Cryptolaemus montrouzieri ; Rodolia cardinalis ; Chilocorus bipustulatus ; Neuroptera ; Chrysopidae ; Chrysoperla carnea ; Hymenoptera ; Encyrtidae ; Encyrtus infelix ; Leguminosae ; Erythrina corallodendrum ; Spartium junceum ; Citrus sinensis ; Euphorbia tirucalli ; Pittosporum tobira
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Interactions were studied among alkaloid-containing legumes (Erythrina corallodendrum andSpartium junceum) and non-toxic plants (Citrus sinensis, Cucurbita moschata andEuphorbia tirucalli), several polyphagous homopterans,Aphis craccivora (Aphididae),Icerya purchasi, I. aegyptiaca (Margarodidae),Lepidosaphes ulmi (Diaspididae) andPlanococcus citri (Pseudococcidae), and some major natural enemies of these homopterans. Significant reductions in survival due to negative effects of alkaloid containing as compared with non-alkaloidal plants were recorded for the predatorsRodolia cardinalis andChilocorus bipustulatus, but not forCryptolaemus montrouzieri (Coleoptera: Coccinellidae),Chrysoperla carnea (Neuroptera: Chrysopidae) andSympherobius sanctus (Neuroptera: Sympherobiidae). The development time of the larvae or pupae ofR. cardinalis, C. carnea andS. sanctus was longer on the toxic plants than on the non-toxic ones. The percentage of parasitism ofA. craccivora collected from the non-alkaloidal plantsVicia palaestina andMelilotus albus was much higher than that onS. junceum. The parasitoid complexes ofA. craccivora differed between both plant groups. The nutritive value of honeydew ofI. purchasi andA. craccivora, as expressed by the life span ofEncyrtus infelix (Hymenoptera: Encyrtidae) adults, was also investigated. Life spans were significantly longer when the wasps fed on honeydew produced on non-alkaloidal plants (C. sinensis andPittosporum tobira) than on alkaloid containing plants whenI. purchasi — but notA. craccivora — was the producer. It is suggested that the chemical defense ofE. corallodendrum andS. junceum is exploited by polyphagous phytophages to reduce predation. In nature, population growth and density of four of the investigated homopterans are conspicuously high when they developed on the alkaloid containing plant species, and very low on non-alkaloid plants. The efficiency of their natural enemies may be reduced by sequestration of alkaloids (or other toxic plant compounds) or their transfer into excreted honeydew. Therefore it is assumed that a generalist phytophagous homopteran may be protected from its natural enemies, although at different rates of efficiency, if it can safely sequester the host allelochemical when it develops on toxic species within its host range.
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  • 15
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    Insectes sociaux 39 (1992), S. 59-72 
    ISSN: 1420-9098
    Keywords: Hymenoptera ; Formicidae ; Lasius niger ; food recruitment ; trail laying behaviour ; collective decision making
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The trail-laying behaviour of foragers of the antLasius niger was observed in the laboratory on a 20 cm bridge between the nest and the food source. We measured both the frequency of trail laying, as defined by the proportion of trips during which trail laying occurred, and its intensity, as defined by the number of marks laid during one bridge crossing. Foragers do not exhibit trail-laying behaviour until a food source is discovered. Trail laying then occurs more or less equally both to and from the nest, and both its frequency and intensity decrease as the recruitment proceeds. Foragers from very small colonies less than a year old appear to have quantitatively the same trail laying behaviour as those from older and much larger colonies. Groups of recruiters and recruits were individually marked. Their trail laying intensity was similar, both for trips to and from the nest, and for an ant's first, second, third and fourth trip. The frequency diminished rapidly with the number of trips made by each individual, and was 2–3 times higher for recruiters than for recruits, for trips both to and from the nest. Even though foragers stop marking after a variable number of passages, they continue to move between the nest and the food source, and other ants start marking. Different foragers appear to have widely different levels of trail laying, although we cannot say whether these differences are stable between different recruitments. Trail laying is strongly affected by the foragers' position on the bridge, especially for ants returning to the nest which lay up to five times more on the segment closest to the source than that closest to the nest. Foragers on a weakly marked trail appear to mark more than those on a well-marked trail. However, this effect is weak and could partly be attributed to their lower speed. Finally, a model using the experimental data gathered on the individuals' trail-laying behaviour reproduced satisfactorily the colony's overall trail laying.
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  • 16
    ISSN: 1420-9098
    Keywords: Kin recognition ; social wasp ; Ropalidia marginata ; evolution of sociality ; Hymenoptera ; Vespidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Female wasps of the tropical primitively eusocial speciesRopalidia marginata are known to discriminate unfamiliar nestmates from unfamiliar non-nestmates outside the context of their nests. Here, we show that when foreign conspecifics are introduced in the context of a nest in laboratory cages, genetic relatives among them are treated by nest inhabitants more tolerantly than non-relatives, but that no foreign conspecifics are accepted into the nests. However, some wasps may leave their nest and join the foreign relatives and non-relatives to found new colonies cooperatively. Very few of the introduced animals are severely attacked or killed; most are allowed to remain in parts of the cage away from the nest. These results suggest that factors other than genetic relatedness may be involved in regulating tolerance and acceptance of foreign conspecifics on a nest and its vicinity. Our results are different from those of similar experiments with ants, which have demonstrated that former nestmates that are removed as pupae and later introduced as adults are either accepted into the nest or attacked and killed. We attribute this difference to the fact that in a primitively eusocial species such asR. marginata, the rules governing tolerance and acceptance of foreign conspecifics must be quite different from those in highly eusocial species. We also attempt to test some predictions of the conspecific acceptance threshold models of Reeve (Am. Nat. 133:407–435, 1989). Our results uphold the predictions of his “fitness consequence submodel” but do not support those of his “interaction frequency sub-model”.
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  • 17
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    Cellular and molecular life sciences 48 (1992), S. 872-874 
    ISSN: 1420-9071
    Keywords: Cerebral reperfusion injury ; oxygen radicals ; lipid peroxidation ; antioxidative enzymes ; stobadine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Lipid peroxidation and activities of antioxidative enzymes were studied in the brain cortex after short (15 min) cerebral ischemia and reperfusion (10 min) in rats. Conjugated dienes (CD) and thiobarbituric acid-reactive substances (TBARS) were significantly elevated in the group of rats with ischemia followed by reperfusion in comparison to the ischemic animals. Superoxide dismutase (SOD) activity significantly increased in the group of animals with ischemia and reperfusion. No significant changes in the activities of glutathione peroxidase (GP) were observed. Stobadine administered before ischemia or before reperfusion decreased the level of TBARS. Stobadine probably prevents malondialdehyde (MDA) formation from hydroperoxide or might elevate the activity of aldehyde dehydrogenase. In contradiction to the findings after long-lasting (4 h) ischemia and subsequent reperfusion1, no decrease in the concentration of CD or in the activity of SOD or GP was found.
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  • 18
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    Aquatic sciences 54 (1992), S. 321-330 
    ISSN: 1420-9055
    Keywords: Algal pigments ; algal communities ; photosynthesis ; Lake Lugano (Lago di Lugano)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A one-year study of phytoplankton, primary production and related physical and chemical factors was made in a Swiss basin of Lake Lugano (Lago di Lugano). The chlorophylls and 12 carotenoids were analyzed with a TLC technique. The carotenoid monitoring was considered to be particularly interesting, because the role of these pigments in freshwater algae is still very poorly documented by field studies. The dependence of photosynthesis on several factors was statistically evaluated. Evidence was found of light-adaptation phenomena. The variations of photosynthetic activity and efficiency largely depended on the light regime in the few days before the field observations and on the cellular content of chlorophylls and single carotenoids, whose concentrations in their turn were closely linked with light, temperature, average cell size, and with the actual species assemblage.
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  • 19
    ISSN: 1423-0445
    Keywords: euglossine flowers ; perfume collection ; euglossophily ; floral fragrant exudates ; nesting behaviour ; mating behaviour ; bee pheromones ; nest mimicry ; signal evolution ; Hymenoptera ; Apidae ; Euglossinae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A fascinating pollination system has been evolved between perfume producing flowers and perfume collecting male euglossine bees in the neotropics. Detailed investigations have contributed to an understanding of the interactions between euglossine males and flowers as a pollination system. The role which the collected perfume plays in the reproductive behaviour of euglossine bees is not fully understood. A favoured hypothesis suggests that the collected fragrances are used as precursors for male sex pheromones and thus serve to attract conspecific males or females. It is not known how perfume collection behaviour evolved. Here, an evolutionary approach presents a new hypothesis which suggests that the evolution of perfume collection in euglossine males is based upon pre-existing signals which were attractive to females and males. It is further suggested that, at the evolutionary outset, flowers mimicked nest sites to deceive nest-seeking euglossine bees. In addition, a comparative study was undertaken on the phenomena of nest-mimicking flowers in related bee families.
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  • 20
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    Cellular and molecular life sciences 48 (1992), S. 902-904 
    ISSN: 1420-9071
    Keywords: Leptopilina boulardi ; Hymenoptera ; Eucoilidae ; parasitoid ; olfaction ; learning ; memory ; olfactometer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract FemaleLeptopilina boulardi wasps, larval parasites ofDrosophila melanogaster, can learn to respond to more than one odour by associating these odours with oviposition experience. These wasps can memorise and respond to at least two different odours, and prefer the last one learnt.
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  • 21
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    Oecologia 89 (1992), S. 147-149 
    ISSN: 1432-1939
    Keywords: Sex ratio ; Polyembryony ; Hymenoptera ; Parasitoid ; Copidosoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We examined brood sex ratios of an undetermined species of Copidosoma. Most broods (65%) were unisexual, with a greater proportion of female broods. Some mixed broods contained extremely small proportions of wasps of the opposite sex. Our results imply that the female-biased sex rations in this species cannot be explained by local mate competition theory.
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  • 22
    ISSN: 1440-1703
    Keywords: Clethra barbinervis ; interspecific difference ; intraspecific variation ; photosynthesis ; SO2 resistance
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    Topics: Biology
    Notes: Abstract The effect of SO2 on the photosynthesis ofClethra barbinervis collected from a smoke-polluted area near the Ashio copper smelter in Tochigi Prefecture was compared withC. barbinervis collected from a nonpolluted district in Tsukuba, Ibaraki Prefecture andQuercus mongolica var.grosseserrata grown in a nonpolluted field in Nagano Prefecture. The plants were exposed to 0.5–1.5 p.p.m. SO2 for 90 min (short-term) and to 0.3 p.p.m. SO2 for 31–39 days (long-term). TheClethra plants from both sites had a lower intrinsic stomatal conductance and photosynthetic rate thanQuercus plants. Short-and long-term fumigation caused stomatal closure inQuercus plants, but had little effect on the stomatal conductance ofClethra plants. Under short-term fumigation, nonstomatal photosynthetic inhibition per unit of absorbed SO2 was smallest inClethra plants from Ashio. Long-term fumigation caused photosynthetic decline and visible foliar injury toQuercus plants, but had no effect onClethra plants from Ashio. Consequently,Clethra plants from Ashio had a higher photosynthetic rate thanQuercus plants after long-term fumigation. These results suggest thatC. barbinervis populations in the smoke-polluted area of Ashio had evolved high SO2 resistance connected with SO2 detoxification ability in mesophyll cells.
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  • 23
    ISSN: 1573-4919
    Keywords: adenylyl cyclase ; antioxidants ; catecholamines ; lipid peroxidation ; reactive oxygen species ; β-receptor
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The irreversible loss of activity of the sarcolemma-localized β-receptor-adenylyl cyclase system (β-RAS) in myocardial ischemia is a well documented phenomenon. Alterations in the sarcolemma (SL) induced by reactive O2 species could be responsible for this loss. Therefore the influence of oxidation of SH-groups and lipid peroxidation induced by Fe2+/Vit. C on the β-RAS activity was studied. During incubation of SL with Fe2+/Vit. C a transient enhancement followed by a continuous loss of the β-RAS activity (isoprenaline-, NaF-, Gpp(NH)p-, forskolin-stimulated and basal activity) was observed. In contrast there occurred a continuous loss of SH-groups and lipid peroxidation, beginning immediately after the start of incubation. Loss of SH-groups and lipid peroxidation as well as changes in the β-RAS did not take place in the presence of the antioxidant t-Butyl-4-hydroxyanisole (BHA) or the Fe2+-chelator EGTA. In view of the known ischemia-induced formation of reactive O2 species our results show that these powerful oxidants could contribute to the modulation of the β-RAS during myocardial ischemia.
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  • 24
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    Molecular and cellular biochemistry 115 (1992), S. 59-62 
    ISSN: 1573-4919
    Keywords: lipid peroxidation ; cell aging ; life span ; population doubling ; subculture interval ; DNA synthesis
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Different concentrations of Fe e+/vitamin C mixtures were used as initiators of lipid peroxidation in diploid fibroblasts from cultured human embryonic lung. Malondialdehyde (MDA) formation in the cell cultures was correlated directly with the concentrations of Fe2+ and vitamine C. Lipid peroxidation was associated with an increase in life-span, decrease in the population doubling time and increase in cellular DNA synthesis. The effects of lipid peroxidation varied inversely with the MDA level. These data showed that low levels of lipid peroxidation retarded several biological properties of cultured cells that are associated with cell aging.
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  • 25
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    Molecular and cellular biochemistry 111 (1992), S. 117-124 
    ISSN: 1573-4919
    Keywords: rat liver microsomes ; lipid peroxidation ; antioxidants ; curcumin ; eugenol ; spice principles
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Polyunsaturated fatty acids (PUFA) are vulnerable to peroxidative attack. Protecting PUFA from peroxidation is essential to utilize their beneficial effects in health and in preventing disease. The antioxidants vitamin E, t-butylhydroxy toluene (BHT) and t-butylhydroxy anisole (BHA) inhibited ascorbate/Fe2+-induced lipid peroxidation in rat liver microsomes. In addition, a number of spice principles, for example, curcumin (5–50 µM) from turmeric, eugenol (25–150 µM) from cloves and capsaicin (25–150 µM) from red chillies inhibited lipid peroxidation in a dose-dependent manner. Zingerone from ginger inhibited lipid peroxidation at high concentrations (〉 150 µM) whereas linalool (coriander), piperine (black pepper) and cuminaldehyde (cumin) had only marginal inhibitory effects even at high concentrations (600 µM). The inhibition of lipid peroxidation by curcumin and eugenol was reversed by adding high concentrations of Fe2+.
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  • 26
    ISSN: 1573-4919
    Keywords: erythropoietin ; lipid peroxidation ; Mg2+-ATPase ; Cu2+-ascorbic acid ; RBC membrane ; spectrin
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of erythropoietin (Ep), a glycoprotein hormone, has been studied on lipid peroxidation induced by Cu2+ and ascorbate in vitro, Mg2+ ATPase activity and spectrin of RBC membrane. Our present investigation reveals that Cu2+ and ascorbic acid increases lipid peroxidation of RBC membrane significantly. It has further been observed that under the same experimental condition spectrin, a major cytoskeleton membrane protein, and Mg2+-ATPase activity of RBC membrane decrease significantly. However, exogenous administration of Ep completely restores lipid peroxidation and Mg2+-ATPase activity and partially recovers spectrin of RBC membrane.
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  • 27
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    Molecular and cellular biochemistry 111 (1992), S. 41-47 
    ISSN: 1573-4919
    Keywords: myocardial injury ; lipid peroxidation ; ascorbic acid ; superoxide scavenger
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Ascorbic acid (AH2) is a potential scavenger of superoxide radical and singlet oxygen. In the guinea pig, marginal AH2 deficiency results in intracellular oxidative damage in the cardiac tissue as evidenced by lipid peroxidation, formation of fluorescent pigment and loss of structural integrity of the microsomal membranes. The oxidative damage does not occur due to lack of enzymatic scavengers of reactive oxygen species such as superoxide dismutase, catalase and glutathione peroxidase. Also, glutathione transferase activity is not decreased in AH2 deficiency. Lipid peroxidation, fluorescent pigment formation and protein modification disappear after AH2 therapy. These results, if extra-polated to human beings, would indicate that chronic subclinical AH2 deficiency may result in progressive oxidative damage which in the long run may lead to permanent degenerative diseases in the heart.
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  • 28
    ISSN: 1573-4919
    Keywords: ischemia ; reperfusion ; brain ; glutathione ; glutathione reductase ; glutathione peroxidase ; lipid peroxidation
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Rats were subjected to bilateral carotid artery occlusion for 30 min, followed by reperfusion for varying time periods. The concentration of reduced and oxidized glutathione, glutathione peroxidase and glutathione reductase were determined in whole brain after varying periods of reperfusion. Lipid peroxidation was also assessed by determining the levels of malondialdehyde (MDA) in the brain. Reperfusion for 1 hr following bilateral carotid artery occlusion resulted in significant decrease in total glutathione (GSH) concentration along with small but significant increase in oxidized glutathione (GSSG) levels. After 4 hr of reperfusion, GSH levels recovered, although GSSG levels remained elevated up to 12 hr of reperfusion. Increase in malondialdehyde levels was also detected in the brain up to 12 hr of reperfusion. Glutathione reductase activity remained significantly low up to 144 hr of reperfusion, while glutathione peroxidase activity remained unaffected. These results demonstrate that oxidative stress is generated in the brain during reperfusion following partial ischemia due to bilateral carotid artery occlusion.
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  • 29
    ISSN: 1573-4919
    Keywords: endurance exercise ; Vit E ; free radicals ; lipid peroxidation ; heart
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Exhaustive endurance exercise in adult female albino rats (C-Ex) increased the generation of free radicals (R ·) in the myocardium, probably through enhanced oxidative mechanisms. Free radical mediated lipid peroxidation measured in the form of tissue MDA content also increased in C-Ex animals, suggesting the exercise-induced oxidative stress in these animals. Dietary supplementation of Vit E, for a period of 60 days significantly increased Vit E incorporation into the serum and myocardium, more so in the myocardium. Vit E supplementation to exercising animals completely abolished the radical production. The protection of Vit E against oxidative stress appears to be not mediated through the improvement of antioxidant mechanisms by enzymes like SOD, catalase and Se-GSH Px. However the non Se-GSH Px, the enzyme involved in the reduction of endoperoxides increased significantly in control and Vit E fed animals in response to exercise. The protection of Vit E against exercise-induced oxidative stress was correlated with its multivarious activities like a) scavenger of free radicals; b) inhibition of lipoxygenases; and c) reduction of peroxides in association with lipoxygenases. These studies indicate that dietary supplementation of Vit E protects the animals from the possible oxidative damages of endurance exercise.
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  • 30
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    Molecular and cellular biochemistry 111 (1992), S. 131-135 
    ISSN: 1573-4919
    Keywords: nonesterified fatty acids ; inhibition ; lipid peroxidation
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Oxygen free radicals damage cells through peroxidation of membrane lipids. Gastrointestinal mucosal membranes were found to be resistant to in vitro lipid peroxidation as judged by malonaldehyde and conjugated diene production and arachidonic acid depletion. The factor responsible for this in this membrane was isolated and chemically characterised as the nonesterified fatty acids (NEFA), specifically monounsaturated fatty acid, oleic acid. Authentic fatty acids when tested in vitro using liver microsomes showed similar inhibition. The possible mechanism by which NEFA inhibit peroxidation is through iron chelation and iron-fatty acid complex is incapable of inducing peroxidation. Free radicals generated independent of iron was found to induce peroxidaton of mucosal membranes. Gastrointestinal mucosal membranes were found to contain unusually large amount of NEFA. Circulating albumin is known to contain NEFA which was found to inhibit iron induced peroxidation whereas fatty acid free albumin did not have any effect. Addition of individual fatty acids to this albumin restored its inhibitory capacity among which monounsaturated fatty acids were more effective. These studies have shown that iron induced lipid peroxidation damage is prevented by the presence of nonesterified fatty acids.
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  • 31
    ISSN: 1573-1561
    Keywords: Atta laevigata ; Hymenoptera ; Formicidae ; territorial pheromone ; agonistic behavior ; Dufour gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The workers of the leaf-cutting antAtta laevigata were found to mark a territory around their nest and along trunk trails. Elsewhere, we reported that the territorial mark had orientation and agonistic functions. The presence of this mark induced an alarm behavior in intruder workers and stimulated the residents' aggressiveness. Of the parts of the body tested, only the extracts from gaster or Dufour gland were able to induce the same agonistic behavior. The compoundsn-heptadecane, (Z)-9-nonadecene, 8,11-nonadecadiene, and (Z)-9-tricosene, secreted by Dufour gland were identified on foraging trails in the field as components of the territorial odor.
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  • 32
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    Journal of chemical ecology 18 (1992), S. 209-221 
    ISSN: 1573-1561
    Keywords: Trillium ; ant dispersal ; Hymenoptera ; Formicidae ; elaiosomes ; lipids ; diglycerides ; triglycerides ; oleic acid ; linoleic acid
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract This study was designed to compare the chemistry of elaiosomes of three sympatric species ofTrillium to discover if there are correlations between chemistry and dispersal rates by ants. We quantified the amount of proteins and neutral lipids and qualitatively analyzed the fatty acids occurring in triglyceride, diglyceride, monoglyceride, and free fatty acid fractions.T. grandiflorum had the highest lipid concentration per milligram of protein and the highest total lipid per elaiosome, followed byT. erectum and then byT. undulatum. Oleic acid (18∶1) was abundant in all lipid fractions of each species. This fatty acid, which is responsible for corpse-carrying behavior in some ants, probably causes ants to pick up diaspores. Linoleic acid (18∶2) is relatively abundant inT. erectum andT. grandiflorum but not inT. undulatum and may cause ants that have picked up diaspores to move (carry) those diaspores back to the nest. Total elaiosome mass and fatty acid composition appear to explain whyT. erectum is dispersed most rapidly and why ants that have picked up elaiosomes move moreT. grandiflorum thanT. undulatum diaspores.
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  • 33
    ISSN: 1573-1561
    Keywords: Phenylalkenals ; Leptogenys spp ; Pogonomyrmex rugosus ; Hymenoptera ; Formicidae ; 2-phenylpropenal ; 2-phenyl-2-butenal ; 2,5-dimethyl-3-(1-methyl) butylpyrazine ; 2,5-dimethyl-3-isopentylpyrazine ; 2,5-dimethyl-3-isopentenyl-6-isopentylpyrazine ; 4-methyl-3-heptanone ; 5-methyl-3-hexanone ; Maillard reaction ; benzaldehyde ; chemoreceptors ; defensive allomones
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Cephalic extracts of two unrelated species of ants,Leptogenys processionalis andPogonomyrmex rugosus, have been found to contain 2-phenylpropenal and 2-phenyl-2-butenal, while two other species related to the first,L. chinensis andL. kitteli, lacked either.L. kitteli also produced a tetrasubstituted pyrazine found previously only in two New Zealand ants in the genusMesoponera. The chemical reactivity of the phenylalkenals suggests their function in repelling attack by predators.
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  • 34
    ISSN: 1573-1561
    Keywords: Philanthus crabroniformis ; Philanthus barbatus ; Philanthus pulcher ; Hymenoptera ; Sphecidae ; beewolf ; mandibular glands ; pheromones ; semiochemicals ; mass spectrometry ; infrared spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The composition of the territorial marking pheromones from mandibular glands of males of the beewolvesPhilanthus crabroniformis, P. barbatus, andP. pulcher have been determined. The structures of the components were elucidated by gas chromatography-mass spectrometry and gas chromatography-Fourier transform infrared spectroscopy. The major compound ofP. crabroniformis is isopropyl tetradecanoate, with somewhat lesser amounts of 2-tridecanone, 3-methyl-3-butenyl tetradecanoate, and 92∶8 (Z)∶(E)-11-eicosen-1-ol. The major compounds ofP. barbatus are ethyl tetradecanoate and hexadecanal, which are present in approximately a 60∶40 ratio. These two compounds comprise over 95% of the neutral lipids. Also present in lesser amounts are ethyl dodecanoate, tetradecanal, hexadecan-1-ol, a Δ x -octadecen-1-ol, and octadecan-1-ol. The major compounds ofP. pulcher are ethyl (Z)-7-hexadecenoate and geranylgeraniol acetate, which comprise nearly 90% of the neutral lipid fraction, with smaller amounts of tetradecanal, pentadecanal, and ethyl hexadecanoate; trace amounts of Δ x hexadecenal, hexadecanal, and octadecanal are also present.
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  • 35
    ISSN: 1573-1561
    Keywords: scanning electron microscopy ; CP/MAS [13C]NMR ; Hymenoptera ; Vespidae ; social wasps ; Polistes metricus ; nest paper ; nest pedicel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The ultrastructure and chemical composition of paper and pedicel from nests ofPolistes metricus that were constructed in the laboratory from known building material were compared to paper and pedicel from nests constructed in the field. Scanning electron micrographs showed the addition of a gluelike secretion from the wasp to the construction material. Solid-state [13C]NMR, elemental analyses, and amino acid analyses indicate that this secretion is a silklike protein with serine, glycine, alanine, and proline comprising 65–73% of the identified residues.
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  • 36
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    Journal of chemical ecology 18 (1992), S. 1901-1912 
    ISSN: 1573-1561
    Keywords: Parasitoid ; pheromone ; bioassay ; wind-tunnel ; Hymenoptera ; Braconidae ; Macrocentrus grandii ; Lepidoptera ; Pyralidae ; Ostrinia nubil-alis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A multicomponent sex pheromone inMacrocentms grandii Goidanich (Hymenoptera: Braconidae) was demonstrated using wind-tunnel and field bioassays. In wind-tunnel bioassays, three Florisii fractions from female wasps (hexane, 5%, and 50% ether in hexane) and one from male wasps (50% ether in hexane) were attractive to males. The hexane and 5% ether in hexane fractions each elicited similar male behavioral responses. These included upwind anemotaxis, casting, landing on the source, wing fanning, and mating attempts between males. The 50% ether in hexane fraction, whether male- or female-derived, initiated a strong flight response by males but few landings on the source. In addition, the 50% ether in hexane fraction was found to synergize strongly the hexane fraction. All females tested in the wind tunnel gave no responses to male- or female-derived extracts. Field tests generally supported the wind-tunnel results, although no field attraction was observed for the 5% ether in hexane fraction.
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  • 37
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    Journal of chemical ecology 18 (1992), S. 271-282 
    ISSN: 1573-1561
    Keywords: Plant-insect interaction ; Pinus sylvestris ; Neodiprion sertifer ; Diprion pini ; Hymenoptera ; Diprionidae ; insect performance ; flavonoids ; taxifolin glucoside ; resin acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Responses of sawfly larvae (Hymenoptera, Diprionidae) to the flavonoid taxifolin glucoside in their host plant were studied in a laboratory experiment. Larvae ofNeodiprion sertifer andDiprion pini were raised from egg hatch to cocoon spinning on two Scots pine (Pinus sylvestris) chemotypes, one without needle taxifolin glucoside (−) and the other containing 2–4% taxifolin glucoside (+). The (+) chemotype had somewhat lower concentrations of needle terpenoids (resin acids) than the (−) chemotype. Current-year needles had higher taxifolin glucoside concentrations than mature needles. There were no differences in survival or body size betweenN. sertifer larvae that fed on the (+) chemotype and those that fed on the (−) chemotype. FemaleD. pini larvae raised on (+) needles developed 6% more slowly than larvae fed (−) needles. The results from this study are contrary to earlier findings showing that flavonoid glucosides have strong negative effects on insect performance. Possible explanations for the different outcomes are discussed.
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  • 38
    ISSN: 1573-5028
    Keywords: gene duplication ; photosynthesis ; RFLP ; Southern blots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A second locus (Lhb1B) encoding Photosystem II Type I chlorophyll a/b-binding (CAB) polypeptides was identified in Arabidopsis thaliana. This locus carries two genes in an inverted orientation. The predicted sequences of the polypeptides encoded by these two genes show substantial divergence in their amino termini relative to each other and to the proteins encoded by the three Lhb1 CAB genes previously characterized [10], but little divergence within the predicted primary structure of the mature protein. DNA probes derived from seven additional types of tomato CAB genes, encoding chlorophyll a/b-binding polypeptides of several antenna systems of the photosynthetic apparatus, were tested against A. thaliana. Each of these hybridized in Southern blots to unique DNA fragment(s), demonstrating the existence of each of these different types of CAB genes in the genome of A. thaliana. The number of genes encoding each CAB type in A. thaliana was estimated to be similar to that of tomato.
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  • 39
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    Plant molecular biology 20 (1992), S. 481-491 
    ISSN: 1573-5028
    Keywords: photosynthesis ; cytochrome b 6 ; gene regulation ; genome mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genes encoding the photosynthetic cytochrome b 6 (petB) and subunit 4 (petD) have been cloned and sequenced from the unicellular, photoheterotrophic, transformable cyanobacterium Synechococcus sp. PCC 7002, formerly designated Agmenellum quadruplicatum. The gene arrangement was found to be similar to that reported in the cyanobacterium Nostoc PCC 7906. The DNA and derived protein sequences were compared to chloroplast and the other cyanobacterial sequences. By pulsed-field electrophoresis, the petBD operon and the petCA operon, encoding the Rieske iron-sulfur protein and cytochrome f, were found to be located on separate, unlinked,Not I-digested DNA fragments. ThepetBD operon was found on the third largest Not I fragment (NC-325) while the petCA operon was found on the second largest Not I fragment (NB-370). These results suggest the two operons are not in proximity. The 1.35 kb transcript was shown to be light-regulated. Transcripts from cells grown under constant illumination showed a decrease in petB transcript levels to undetectable levels within 2 h after the cells were placed in the dark. Upon reillumination, transcript levels rose to three-fold over that seen initially under constant illumination.
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  • 40
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    Biochemical genetics 30 (1992), S. 443-453 
    ISSN: 1573-4927
    Keywords: heterozygosity ; allozymes ; Hymenoptera ; Bombus ; Psithyrus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Allozyme variation at an average of 37.3 loci was assessed in queens of 16Bombus and 2Psithyrus bumble bee species from North America. The mean expected heterozygosity $$(\bar H)$$ for theBombus species was 0.008±0.006 (95% confidence limits) and that for thePsithyrus was 0.007±0.007. These levels are significantly lower than found in other Hymenoptera but are comparable to those found in previous studies of bumble bees based on far fewer loci. Neutral mutation and random genetic drift can account for the observed variation, but this implies a very small effective population size for species of bumble bees.
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  • 41
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    Plant molecular biology 19 (1992), S. 217-230 
    ISSN: 1573-5028
    Keywords: chlorophyll a/b-binding protein genes ; Glycine ; LHCP II ; photosynthesis ; soybean ; ribulose-1,5-bisphosphate carboxylase genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The levels of chlorophyll a/b-binding protein (Cab) gene polysomal poly(A)+ mRNA were quantitated throughout the development of Glycine max L. Cab mRNAs were abundant in young expanding leaves, representing 6.1% of the leaf mRNA population. Lower Cab mRNA levels were present in embryos, stems, and cotyledons of developing seedlings; the lowest levels were found in roots where they accounted for 0.04% of the polysomal poly(A)+ mRNA of this organ. To determine the contribution of different members of the Cab gene family to the Cab mRNA populations, a quantitative S1 nuclease reconstruction assay was developed. Cab3, Cab4, and Cab5 mRNAs were detected in all stages examined during soybean development but their levels underwent differential changes. Cab3 encodes the most abundant Cab mRNA in young leaves, developing embryos, and in Stage VII cotyledons from the developing soybean seedling. The levels of Cab mRNAs were compared to the levels of ribulose-1,5-bisphosphate carboxylase small subunit gene mRNA and differences in their patterns of accumulation were noted. Collectively these data indicate that during soybean embryogenesis developmental control mechanisms supersede light-regulatory signals.
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  • 42
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    The journal of membrane biology 126 (1992), S. 265-275 
    ISSN: 1432-1424
    Keywords: photosynthesis ; thylakoids ; electrochromism ; gramicidin ; conductance ; dimerization
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The effect of the pore-forming antibiotic gramicidin on pure lipid membranes is well characterized. We studied its action in protein-rich thylakoid membranes that contain less than 25% (wt/wt) acyl lipids. A transmembrane voltage was induced by flashing light, and its decay was measured and interpreted to yield the distribution of gramicidin over thylakoids, its dimerization constant and its single-channel conductance in this membrane. The distribution of gramicidin over the ensemble of thylakoids was immediately homogeneous when the antibiotic was added under stirring, while it became homogeneous only after 20 min in a stirred suspension that was initially heterogeneous. The dimerization constant, 5×1014 cm2/mol, was about 10 times larger than in pure lipid membranes. This was attributed to the upconcentration of gramicidin in the small fractional area of protein free lipid bilayer and further by a preference of gramicidin for stacked portions of the membrane. The latter bears important consequences with regard to bioenergetic studies with this ionophore. As gramicidin was largely dimerized from a concentration of 1 nm (in the suspension) on, the membrane's conductance then increased linearly as a function of added gramicidin. When the negative surface potential at the thylakoid membrane was screened, the conductance of a single gramicidin dimer agreed well with figures reported for bilayers from neutral lipid (about 0.5 pS at 10 mm NaCl). The modulation of the conductance by the surface potential in spinach versus pea thylakoids and between different preparations is discussed in detail.
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  • 43
    ISSN: 1432-1351
    Keywords: Comparative colour vision ; Opponent processes ; Colour computation ; Hymenoptera ; Colour discrimination behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Behavioural tests were carried out with 9 hymenopteran insect species, which ranked certain sets of coloured stimuli according to their subjective similarity to a previously memorized stimulus. Kendall's τ coefficient is employed for the analysis of correlation between these similarity rankings and the colour distance rankings predicted by various models of neural colour computation. The models are based on the measured spectral sensitivities of photoreceptor colour types and use a variety of simple colour coding systems to derive hypothetical colour distances. The correlation between the predictions of the models and the behavioural results serves as a measure for the likelihood of existence of a colour coding system. In all species, the similarity rankings can be best explained by assuming that colour is coded on a perceptual level by two colour opponent mechanisms. Brightness differences are ignored, indicating that an intensity-coding sub-system is not used in colour discrimination by the insects investigated. The weighting factors of the colour opponent mechanisms differ between species in detail, but not in the principles involved. It is thus possible to employ a standard measure of perceptual colour distance (colour hexagon distance) to predict the capacities of colour discrimination adequately in all the tested insects.
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  • 44
    ISSN: 1573-5176
    Keywords: adaptation ; β-carotene ; Dunaliella salina ; photosynthesis
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    Topics: Biology
    Notes: Abstract Photosynthetic characteristics of Dunaliella salina with high (red form) and low β-carotene (green form) concentrations were studied. D. salina growing in brine saltworks exhibited a high level of β-carotene (15 pg cell−1). The rate of oxygen evolution as a function of irradiance was higher in the red than in the green form (on chlorophyll basis). Photosynthetic inhibition of the green form was observed above 500 µmol m−2 s−1. The red form appeared more resistant to high irradiance and no inhibition in O2 evolution was observed up 2000 µmol m−2 s−1. However, when these results are expressed on a cell number basis the rate of oxygen evolution was significantly higher in the green form. Carbonic anhydrase (CA) activity (total, soluble, membrane bound) was found in red and green forms. CA was higher in the red form on a chlorophyll basis, but lower if expressed on a protein basis. The light dependent rate of oxygen evolution and photoinhibition depends on the concentration of β-carotene in D. salina cells.
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  • 45
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    Journal of applied phycology 4 (1992), S. 1-9 
    ISSN: 1573-5176
    Keywords: photobioreactor ; flat plate air-lift reactor ; Chlorella ; Synechococcus ; hydraulic characteristics ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A flat plate, multi-pass air lift reactor (FPALR) for the culture of photosynthetic organisms was constructed from twin wall acrylic sheet and its performance characterised. When operated at an air input of 2.01 min−1 the multi-pass system had a Reynolds number of 5200 indicating fully turbulent flow. Chlorella vulgaris 211/11c was found to have a stationary phase biomass of 1.48 g 1−1 when grown in the flat plate air lift reactor (FPALR) at 100 µmol m−2s−1 compared to 1.11 g 1−1 when cultured in the continually stirred tank reactor (CSTR) at the same PFD (photon flux density). The same organism cultured at 200 µmol m−2s−1 achieved a stationary phase biomass of 1.71 g 1−1 in the FPALR. In contrast, Scenedesmus sp. produced a stationary phase biomass of 2.27 g1−1 and 1.27 g1−1, when cultured at 100 µmol m−2s−1 in the FPALR and the CSTR respectively. The growth rates of both organisms were also higher in the PFALR.
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  • 46
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    Evolutionary ecology 6 (1992), S. 312-330 
    ISSN: 1573-8477
    Keywords: genetic models ; inbreeding depression ; mating cost ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Existing genetic models of the evolution of sibmating behaviour in diploids incorporate inbreeding depression in terms of reduced fecundity of consanguineous mating pairs rather than reduced survival or fecundity of the progeny of such matings. Here we derive a model to correct this deficiency and extend the model to haplodiploids where differential effects of inbreeding in males and females is a crucial consideration. Our analyses indicate that sibmating can readily evolve in both diploids and haplodiploids in which male mating costs and inbreeding depression are reasonably low, provided there is some mechanism to permit sibmating such as siblings being reared in nests or other forms of aggregation. Our analyses also indicate that once sibmating invades, it typically will go to fixation, although sib-/randommating polymorphisms can persist in both diploids and haplodiploids if male mating costs are close to zero and inbreeding depression reduces survival by around one-third. The conditions favouring sibmating are slightly more restrictive in haplodiploids than in diploids. In light of this we may ask why we see intense sibmating in many haplodiploids such as parasitic wasps, fig wasps, ants, bark beetles and mites, and only rarely in diploid animals. The common factor could be certain kinds of aggregation behaviour that are a prerequisite for sibmating in the absence of kin recognition. Another possibility is that inbreeding depression is likely to be more severe in diploids than in haplodiploids because deleterious recessives are purged from haplodiploid populations when expressed by haploid males. Thus, lower levels of inbreeding depression might be one important reason why sibmating appears to arise more frequently in haplodiploids than diploids. Phylogenetic analysis of groups, such as bark beetles and mites, exhibiting both diploid and haplodiploid populations may be useful in elucidating the relative importance of gregarious behaviour and haplodiploidy in facilitating sibmating systems.
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  • 47
    ISSN: 1573-5079
    Keywords: carotenoid ; chlorophyll fluorescence ; non-photochemical quenching ; pH ; photosynthesis ; zeaxanthin
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    Notes: Abstract The pH dependence of maximum chlorophyll fluorescence yield (Fm) was examined in spinach thylakoids in the presence of nigericin to dissipate the transthylakoid pH gradient. 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) was present to eliminate photochemical quenching. Thylakoids were prepared from dark adapted leaves (‘dark’ thylakoids) or preilluminated leaves (‘light’ thylakoids). In the latter there had been approximately 50% conversion of the xanthophyll violaxanthin to zeaxanthin, while no conversion had occurred in the former. In the presence of a reductant such as ascorbate, antimycin A sensitive quenching was observed (half maximal quenching at 5 μM), whose pH dependence differed between the two types of thylakoid. Preillumination of leaves resulted in more quenching at pH values where very little quenching was observed in ‘dark’ thylakoids (pH 5–7.6). This was similar to activation of high-energy-state quenching (qE) observed previously (Rees D, Young A, Noctor G, Britton G and Horton P (1989) FEBS Lett 256: 85–90). Thylakoids isolated from preilluminated DTT treated leaves, that contained no zeaxanthin, behaved like dark thylakoids. A second form of quenching was observed in the presence of ferricyanide, that could be reversed by the addition of ascorbate. This was not antimycin A sensitive and showed the same pH dependence in both types of thylakoid. The former type of quenching, but not the latter, showed similar low temperature fluorescence emission spectra to qE, and was considered to occur by the same mechanism.
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  • 48
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    Photosynthesis research 33 (1992), S. 163-170 
    ISSN: 1573-5079
    Keywords: biosynthetic pathway ; evolution ; free energy ; photochemistry ; photosynthesis ; porphyrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Living matter is an organized system which requires a continual flux of energy for its survival. As a working assumption, the flux of energy required for the origin of a self-duplicating cell is taken as the power required for the maintenance of a modern cell: 10 mW per g of carbon or some 105 times the output per gram of the sun. Solar photochemistry supplies the energy for the continuing evolution of life and, by continuity, for its origin. The iron oxide-sulfide photosynthetic unit proposed by S. Granick 35 years ago was meant to supply this energy. The evolution of complex organic photosensitizers is rationalized by the Granick hypothesis that biosynthetic pathways recapitulate their evolution. These concepts are discussed in the context of the evolution of photosynthetic systems and the known properties of these pigments.
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  • 49
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    Photosynthesis research 32 (1992), S. 139-146 
    ISSN: 1573-5079
    Keywords: leaf senescence ; mesophyll conductance ; Oryza sativa L. ; photosynthesis ; specific leaf weight ; stomatal conductance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The grain yield of rice (Oryza sativa L.), as well as of other cereal crops, is limited to a large extent, by the supply of photosynthates produced during grain filling period. In this study, flag leaf photosynthesis (LPS) after heading was compared among 32 cultivars bred during the past century in Japan, to determine if the improvement of LPS has occurred with the breeding advance of high yielding cultivars. Measurement of LPS was made for 5 consecutive years in the paddy field, on the flag leaf of the main stem, at heading (LPS-0), and 2 weeks (LPS-2) and 4 weeks (LPS-4) after heading. LPS decreased with advance of leaf senescence from LPS-0 to LPS-2, and then to LPS-4. However, if nitrogen was top-dressed at the heading time, high LPS-2 was maintained, particularly in the newer cultivars. A significant positive correlation between LPS and the released year of cultivar was found at LPS-2, especially in the nitrogen top-dressed plot, but not at LPS-0 or LPS-4. Cultivar difference in LPS of the senescing leaves were not stable through the different years, whereas LPS-0 was stable over years, suggesting that the LPS in the senescent leaf is susceptible to the environmental variation due to the effects on leaf senescence. Cultivar difference in LPS at any stage was closely associated with mesophyll conductance to CO2, and stomatal conductance was also associated with cultivar difference in such a high LPS as LPS-0 and nitrogen top-dressed LPS-2. Significant correlation between LPS and specific leaf weight was not observed at any stage of the flag leaf.
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  • 50
    ISSN: 1573-5079
    Keywords: chlorophyll fluorescence ; Dunaliella ; photosynthesis ; quantum efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The yield of photosynthetic O2 evolution was measured in cultures of Dunaliella C9AA over a range of light intensities, and a range of low temperatures at constant light intensity. Changes in the rate of charge separation at Photosystem I (PS I) and Photosystem II (PS II) were estimated by the parameters ΦPS I and ΦPS II . ΦPS I is calculated on the basis of the proportion of centres in the correct redox state for charge separation to occur, as measured spectrophotometrically. ΦPS II is calculated using chlorophyll fluorescence to estimate the proportion of centres in the correct redox state, and also to estimate limitations in excitation delivery to reaction centres. With both increasing light intensity and decreasing temperature it was found that O2 evolution decreased more than predicted by either ΦPS I or ΦPS II. The results are interpreted as evidence of non-assimilatory electron flow; either linear whole chain, or cyclic around each photosystem.
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  • 51
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    Photosynthesis research 33 (1992), S. 171-176 
    ISSN: 1573-5079
    Keywords: photosynthesis ; origin of life ; clays
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    Topics: Biology
    Notes: Abstract The origin of photosynthesis is speculated to have involved carbon dioxide and self-replicating iron-rich clays. The later evolution of photosynthesis is considered to have undergone four distinct phases: (1) The photoreduction of carbon dioxide by ferrous ion to form oxalate and formate. (2) The entry of sulfur into the evolving clay system which led to the formation of acetyl thioesters. The polymerization of the acetyl thioeters led to the formation of quinones. The formation of Fe2S2 and Fe4S4 cores appeared in this phase. (3) The ability to fix nitrogen characterized the third phase. This led to the formation of pyrrole, flavin, nicotinamide, phycobilins, porphyrins and chlorophyll. (4) Finally, phosphate entered the evolving system. The chromophores evolved from ferrous ion through the quinones, carotenoids, phycobilins to chlorophyll. This evolution of chromophores implies that photosynthesis began in the UV and evolved through the blue, yellow, orange into the red. The electron transport chain evolved from ferrous ion through the Fe2S2 and Fe4S4 cores to the hemes.
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  • 52
    ISSN: 1573-5079
    Keywords: Chlamydomonas ; convexity ; chlorophyll a fluorescence ; Photosystem II heterogeneity ; photoinhibition ; photosynthesis ; PS II repair cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of a 60 min exposure to photosynthetic photon flux densities ranging from 300 to 2200 μmol m−2s−1 on the photosynthetic light response curve and on PS II heterogeneity as reflected in chlorophyll a fluorescence were investigated using the unicellular green alga Chlamydomonas reinhardtii. It was established that exposure to high light acts at three different regulatory or inhibitory levels; 1) regulation occurs from 300 to 780 μmol m−2s−1 where total amount of PS II centers and the shape of the light response curve is not significantly changed, 2) a first photoinhibitory range above 780 up to 1600 μmol m−2s−1 where a progressive inhibition of the quantum yield and the rate of bending (convexity) of the light response curve can be related to the loss of QB-reducing centers and 3) a second photoinhibitory range above 1600 μmol m−2s−1 where the rate of light saturated photosynthesis also decreases and convexity reaches zero. This was related to a particularly large decrease in PS IIα centers and a large increase in spill-over in energy to PS I.
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  • 53
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    Photosynthesis research 34 (1992), S. 249-262 
    ISSN: 1573-5079
    Keywords: photosynthesis ; light-harvesting chlorophyll-protein complex ; spinach ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The monomeric chlorophyll-protein complexes, CP 29 and CP 26 seen in the Camm and Green (1980) and Dunahay and Staehelin (1986) green gels do not always migrate in the order of the apparent molecular weight of their apoproteins as determined by denaturing gel electrophoresis. In barley and corn they do, but in spinach they do not. In addition, in some higher plant species these chlorophyll-protein complexes comigrate on green gels causing confusion in the literature. To remedy this situation and circumvent future confusion, we propose that the CP 29 and CP 26 complexes be named according to the relative molecular weight of their apoproteins on denaturing gels. Our proposal is supported by the results obtained from four antibodies used on Western blot samples of whole thylakoids, grana membranes, and PS II preparations from different plants. The higher molecular weight proteins (proposed CP 29's) react strongly to one set of antibodies, and the lower molecular weight proteins (proposed CP 26's) react strongly to a different set. In spinach, CP 26 antibodies react also with CP 29, but the extent of the cross-reactivity depends critically on the gel electrophoresis system used. Accordingly, a lack of antibody reactivity under certain conditions may not indicate two proteins are unrelated, just simply that a particular epitope is no longer accessible following gel electrophoresis with a particular buffer system.
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  • 54
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    Photosynthesis research 34 (1992), S. 387-395 
    ISSN: 1573-5079
    Keywords: oscillations ; photosynthesis ; regulation ; phosphate ; down-stream
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  • 55
    ISSN: 1573-5079
    Keywords: chlorophyll fluorescence ; cyclic electron transport ; photorespiration ; photosynthesis ; Photosystem II ; proton gradient
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlorophyll fluorescence, light scattering, the electrochromic shift P515 and levels of some photosynthetic intermediates were measured in illuminated leaves. Oxygen and CO2 concentrations in the gas phase were varied in order to obtain information on control of Photosystem II activity under conditions such as produced by water stress, when stomatal closure restricts access of CO2 to the photosynthetic apparatus. Light scattering and energy-dependent fluorescence quenching indicated a high level of chloroplast energization under high intensity illumination even when linear electron transport was curtailed in CO2-free air or in 1% oxygen with 35 μll-1 CO2. Calculations of the phosphorylation potential based on measurements of phosphoglycerate, dihydroxyacetone phosphate and NADP revealed ratios of intrathylakoid to extrathylakoid proton concentrations, which were only somewhat higher in air containing 35 μl l-1 CO2 than in CO2-free air or 1% oxygen/35 μl l-1 CO2. Anaerobic conditions prevented appreciable chloroplast energization. Acceptor-limitation of electron flow resulted in a high reduction level of the electron transport chain, which is characterized by decreased oxidation of P700, not only under anaerobic conditions, but also in air, when CO2 was absent, and in 1% oxygen, when the CO2 concentration was reduced to 35 μll-1. Efficient control of electron transport was indicated by the photoaccumulation of P700 + at or close to the CO2 compensation point in air. It is proposed to require the interplay between photorespiratory and photosynthetic electron flows, electron flow to oxygen and cyclic electron flow. The field-indicating electrochromic shift (P515) measured as a rapid absorption decrease on switching the light off followed closely the extent of photoaccumulation of P700 + in the light.
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  • 56
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    Hydrobiologia 238 (1992), S. 189-196 
    ISSN: 1573-5117
    Keywords: photosynthesis ; phytoplankton ; time scales ; environmental variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Static P(I) curves relating photosynthesis to the instantaneous light might not be adequate to describe the activity of algal cells in lakes or oceans where mixing can cause a complex pattern of light variation. In recent years experimental results have provided evidence that, subsequent to changes in light, the rate of photosynthesis may be delayed or exhibit complex temporal dynamics. The model DYPHORA (DYnamic model for the PHOtosynthetic Rate of Algae) takes these dynamics into account by introducing two characteristic response times for the biological processes: (1) the effect of light inhibition having a time scale of minutes to a few hours and (2) the time lag of the rate of photosynthesis for increasing light intensities having a time scale of seconds to minutes. The importance of the dynamic relative to the static description of photosynthesis depends on the time scales of the changes in the environment and the biological response, becoming significant when the time scales are comparable.
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  • 57
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    Hydrobiologia 248 (1992), S. 215-234 
    ISSN: 1573-5117
    Keywords: suspended solids ; turbidity ; algae ; photosynthesis ; light ; mining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Placer gold-mining on the West Coast of the South Island of New Zealand provided a field test-bed for investigating the impacts of fine inorganic suspensoids (clays) on streams not subjected to other abuses. The suspensions of clays (40% between 0.55 and 1 µm in diameter) seeping into the streams from gold mines were colloidally stable. The clay particles attenuated light in the streamwater with near maximum efficiency leading to severe degradation of stream optical quality. Turbidity increased from a median of 2.4 NTU upstream often to 〉 100 NTU (median 15 NTU) downstream. The stream waters, which were strongly-coloured by humic substances, were changed from a dark organge colour to a bright ‘muddy’ appearance downstream of mining, and visual clarity was reduced from a few metres to as low as 0.03 m (median 0.33 m). The clay discharges decreased light penetration into the stream water such that irradiance averaged over a 12 hr photoperiod at the bed (typically about 0.3 m depth in runs at baseflow) fell from about 340 µE m−2 s−1 upstream to as low as 80 µE m−2 s−1 (median 190 µE m−2 s−1) at matched downstream sites. This reduction in light proportionally reduced benthic primary productivity downstream of the mining activity. In turn this reduced benthic algal biomass and lowered the phototrophic content of the epilithon. In spite of their extremely low settling velocities (〈 1 µm s−1) some clay particles were deposited on the stream bed owing to entrapment in the epilithon matrix. This decreased the organic content of the epilithon (from an average of 19% upstream to 8.5% downstream) so reducing its quality as food for invertebrate animals.
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  • 58
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    Hydrobiologia 231 (1992), S. 93-98 
    ISSN: 1573-5117
    Keywords: photosynthesis ; macroalgae ; deep-water ; Bermuda ; Dictyotales
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetic performance and dark respiration rates were determined in situ for abundant macroalgae occurring between 27–49 m depths off Bermuda. Brown algae, particularly members of the order Dictyotales, predominated at all deep-water sites, and Stypopodium zonale was the most abundant species. Species showed net photosynthesis at very low ambient irradiances (〈0.01 maximum Io). Lobophora variegata, a species with a highly decumbent growth form, had low productivity across all irradiances. In contrast, Dictyota spp. (D. bartayresii, D. dichotoma, D. divaricata) and S. zonale had high photosynthetic capacity (ca. 400 µmol O2 gdw−1 h−1), and light saturation was not evidenced at the highest ambient irradiance level (300 µE m−1 s−1) for species with thin, flat thalli. Light-harvesting pigment concentrations reflected tissue-nitrogen levels. C:N atom ratios, except for L. variegata and D. divaricata, were within the ratio for balanced growth. The repeated occurrence and photosynthetic efficiency of these Dictyotalean species in subtropical and tropical deep-water habitats emphasize their successful adaptation to low-light, nutrient-poor environments.
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  • 59
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    Hydrobiologia 238 (1992), S. 1-35 
    ISSN: 1573-5117
    Keywords: periodicity ; diel ; circadian ; clocks ; photosynthesis ; phytoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Daily variation in phytoplankton productivity influences the dynamics and linkages between several large scale processes in aquatic ecosystems. As part of an opening address to the 5th International workshop for the Group for Aquatic Productivity (GAP), the daily patterns of variability in photosynthesis for different algal classes was introduced and accompanied by a discussion of the sources of environmental and endogenous regulation of repeating biological oscillations that occur in phytoplankton on timescales of one day. It is suggested that one way to develop a database that serves to sort and predict phytoplankton variability over the day may be to encourage the creation of a ‘temporal library’. Such a library would be comprised of temporally fixed maps of circadian clock-controlled rhythms for individual species, as well as temporally variable maps of diel periodicities that only can be defined for a selected set of environmental conditions.
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  • 60
    ISSN: 1573-5117
    Keywords: picoplankton ; photosynthesis ; primary production ; cyprinids ; fish farm pond
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    Topics: Biology
    Notes: Abstract The phytoplankton dynamics of a Chinese integrated fish culture pond in the suburbs of Shanghai were studied in September and October 1989. The chlorophyll a concentration was high with a range of 62.5–127.3 µg l−1; however, daily net production of phytoplankton was relatively low, with a range of 0.53–1.94 gC m −2 d−1. Of the total phytoplankton biomass, 70–87% was composed of nanoplankton (〈10 µm) and picoplankton, probably because of the selective feeding by phytoplanktivorous carp. In particular, the chlorophyll a concentration of picoplankton was 2.1 – 14.1 mg m −3, and its contribution to total phytoplankton production rate was high (18–68%).
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  • 61
    ISSN: 1573-5117
    Keywords: photosynthesis ; alpine lake ; phytoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetic activity by phytoplankton was measured during the ice-free seasons of 1984, 1985 and 1987 using the 14C radioassay in high altitude Emerald Lake (California). Relative quantum yield (αB) and light-saturated chlorophyll-specific carbon uptake (Pm B) were calculated from the relationship of light and photosynthesis fitted to a hyperbolic tangent function. Temporal changes in Pm B showed no regular pattern. Seasonal patterns of αB generally had peaks in the summer and autumn. Phytoplankton biomass (as measured by chlorophyll a) and light-saturated carbon uptake (Pm) had peaks in the summer and autumn which were associated with vertical mixing. Estimates of mean daily carbon production were similar among the three years: 57 mg C m−2 2 d−1 in 1984, 70 mg C m−2 2 d−1 in 1985 and 60 mg C m−2 d−1 in 1987. Primary productivity in Emerald Lake is low compared to other montane lakes of California and similar to high-altitude or high-latitude lakes in other regions.
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  • 62
    ISSN: 1573-5117
    Keywords: light/dark cycles ; photosynthesis ; Chlorella ; diurnal variations ; respiration
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    Topics: Biology
    Notes: Abstract Chlorella pyrenoidosa was grown in three continuous cultures each receiving a different light regime during the light period of a diurnal cycle. Hourly samples taken during the light period were subjected to medium frequency light/dark oscillations of equal duration, ranging from 3 to 240 seconds. The oxygen consumption and production of each sample were measured with an oxygen electrode in a small oxygen chamber. Although the light/dark cycles had little overall influence on photosynthetic activity, the microalgae appeared to adapt to the light regime to which they were subjected. Large differences were found between the maximum chlorophyll-specific production rates (P infmax supB ), the chlorophyll-specific production rates (PB) and the respiration rates between the cultures and treated subsamples. Respiration rates increased during the light period, whilst PB either increased, or had a mid light period minimum or maximum. The culture which received an hourly light oscillation during the light period had the highest P infmax supB and lowest respiration rates, and it is suggested that these algae react as in nature, whereas either a sinusoidal or a block light pattern is ‘unnatural’. The latter light regime is commonly used in laboratory studies.
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  • 63
    ISSN: 1573-5117
    Keywords: dynamic light regime ; photosynthesis ; respiration ; quantum requirement
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    Notes: Abstract Comparisons were made of photosynthesis in three light limited cyclostat cultures (LD = 8:16, dilution rate 0.7 d−1) of Chlorella pyrenoidosa, differing only in the dynamics of irradiance supply: as a constant rate, i.e. a block culture; as a sine function of the light period, i.e. a sinusoidal culture; as an 8 h sine function superimposed by an 1 h sine function, i.e. an oscillating culture. The sinusoidal culture had a constant minimum quantum requirement for oxygen evolution (QR) of 10.8 over the photoperiod. The OR of the oscillating culture increased from 24 to 37 during the photoperiod. From changes in α and P max we suggest that: (1) photosynthetic units (PSU) of the block and sinusoidal sulture increased in number; (2) increasingly fewer chlorophyll molecules participated in oxygenic photosynthesis with a decreasing turnover time of the PSU's during an oscillating photoperiod. Values of I k decreased slightly in the block culture, increased slightly in the sinusoidal culture and showed a twofold increase in the oscillating culture. From the ratio of in situ oxygen production (qO2) and P max we infer a balanced equilibrium between photosystem activity and electron transport capacity for the block and sinusoidal culture. We hypothesize that the qO2 values of the oscillating culture underestimated true oxygen production rates due to a nonlinear response at peak light intensities. The results show that a dynamical photoperiod provoked significantly different photosynthetic responses, even though the overall growth rate was unaffected.
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  • 64
    ISSN: 1573-5117
    Keywords: streams ; arctic ; tundra ; epilithon ; photosynthesis ; respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthesis and respiration by the epilithic community on cobble in an arctic tundra stream, were estimated from oxygen production and consumption in short-term (4–12 h), light and dark, chamber incubations. Chlorophyll a was estimated at the end of each incubation by quantitatively removing the epilithon from the cobble. Fertilization of the river with phosphate alone moderately increased epilithic chlorophyll a, photosynthesis, and respiration. Fertilization with ammonium sulfate and phosphate, together, greatly increased each of these variables. Generally, under both control and fertilized conditions, epilithic chlorophyll a concentrations (mg m−2), photosynthesis, and respiration (mg O2 m−2, h−1) were higher in pools than in riffles. Under all conditions, the P/R ratio was consistent at ∼ 1.8 to 2.0. The vigor of epilithic algae in riffles, estimated from assimilation coefficients (mg O2 [mg Chl a]−1 h−1) was greater than the vigor of epilithic algae in pools. However, due to the greater accumulation of epilithic chlorophyll a in pools, total production (and respiration) in pools exceeded that in riffles. The epilithic community removed both ammonium and nitrate from water in chambers. Epilithic material, scoured by high discharge in response to storm events and suspended in the water column, removed ammonium and may have increased nitrate concentrations in bulk river water. However, these changes were small compared to the changes exerted by attached epilithon.
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  • 65
    ISSN: 1573-6881
    Keywords: Na-ATPase ; ouabain-insensitive ; lipid peroxidation ; basolateral plasma membranes ; apparent energy of activation ; lysophospholipids ; rat kidney cortex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The present work evaluates the effect of lipid peroxidation on the ouabain-insensitive Na-ATPase of basolateral plasma membranes from rat kidney proximal tubular cells as an indirect way to study the lipid dependence of this enzyme. An inverse relationship between lipid peroxidation and Na-ATPase activity was found. This effect was due neither to a change in the optimalK m of the system for Na+ nor for the substrate Mg : ATP, nor the optimal pH value of the medium. The optimal temperature value, however, was shifted toward a higher value. There was also an increase of the apparent energy of activation in the region of temperatures above the transition point (20°C) with increase in lipid peroxidation. Peroxidized membranes incubated with phosphatidylcholine from soybean restored their Na-ATPase activity. On the other hand, the Na-ATPase activity was sensitive to oleoly lysophosphatidylcholine. These results suggest that lipid peroxidation might be affecting the Na-ATPase activity through either an increase of peroxidized phospholipids, which might change the membrane fluidity of the lipid microenvironment of the ATPase molecules, or through a direct effect of lysophospholipids released during the lipid peroxidation.
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  • 66
    ISSN: 1440-1703
    Keywords: carbon isotope ratio ; photosynthesis ; phytoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Primary production by phytoplankton in the eutrophic Mikawa Bay, Japan, was studied by simultaneous measurements of natural carbon isotope ratio (δ 13C) and short-term carbon uptake rates (13C tracer study) of size-fractionated nannoplankton (〈10 μm) and net plankton (〉10 μm) samples. Short-term photosynthetic rates, which represent the physiological state of algae, were variable regardless of standing stock sizes. Theδ 13C values of particulate organic carbon (POC) in June and July displayed horizontal variations for both the net plankton fraction (−19.8 to −12.7‰) and the nannoplankton fraction (−22.0 to −12.8‰). For both fractions, low concentrations of POC had more negativeδ 13C values (−22 to −18‰). Highδ 13C values for the net plankton were found when POC concentrations were much higher, due to red tide. This suggests that the increase in algal standing crop for the net plankton fraction resulted from accelerated photosynthetic activity. However the nannoplankton fractions with higher POC values have relatively lowδ 13C values.
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  • 67
    ISSN: 1573-1561
    Keywords: Polistes exclamans ; Hymenoptera ; Vespidae ; cuticular hydrocarbons ; discriminant analysis ; nest pedicel ; nestmate recognition ; social wasps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The articular lipids were analyzed from individualPolistes exclamans workers collected from 10 nests. Surface lipids were also recovered from the paper and pedicels of these nests. Twenty-two hydrocarbons were identified in these lipid fractions. The major components of the wasp cuticular lipids weren-heptacosane,n-nonacosane, methylhentriacontane, and methyltritriacontane. Discriminant analysis of the hydrocarbon profiles of the adult wasps showed that the wasps group together according to their respective colonies. Several colonies from the same geographical location clustered more closely together than colonies from diverse locations. The nest papers and pedicels did not group with the wasps from their respective colonies, but the nest papers clustered together separately, as did the nest pedicels.
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  • 68
    ISSN: 1573-1561
    Keywords: Argentine ants ; Iridomyrmex humilis ; Hymenoptera ; Formicidae ; semiochemicals ; ant repellents ; farnesol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Foraging in trees by the Argentine ant,Iridomyrmex humilis (Mayr), was disrupted by a variety of synthetic chemicals, with the most effective chemical being farnesol. Testing of substrates for presentation of the disruptant chemicals gave some success with rubber or Tygon tubing, although best results were obtained through incorporation of the material into Stikem, which was then banded around tree trunks. Amounts of farnesol used for effective, long residual ant control were between 0.8 and 2 g per tree.
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  • 69
    ISSN: 1573-1561
    Keywords: Ants ; Hymenoptera ; Formicidae ; poison gland ; Dufour gland ; alkylpyrazines ; tetramorine ; trail following
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The poison gland, which is the source of the trail pheromone inDaceton armigerum, contains a mixture of dimethylpyrazine, trimethylpyrazine and 3-ethyl-2,5-dimethylpyrazine in approximately equal proportions, similar to that of the antManica rubida. The cross-activity in trail-following tests with several other species can thus be explained. The Dufour gland ofD. armigerum contains a mixture of 26 identified hydrocarbons, chiefly 9-tricosene, tricosane, and 9-pentacosene. It also contains very small amounts of two sesquiterpene aldehydes, tetramorine-2 and tetramorine-3, already known in other myrmicine Dufour glands. No volatile substances were detected in the pygidial gland.
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  • 70
    ISSN: 1573-1561
    Keywords: Alfalfa seed chalcid ; Bruchophagus roddi ; Hymenoptera ; Eurytomidae ; alfalfa volatiles ; plant odors ; electroantennogram ; chemoreception ; olfaction ; kairomones ; host plant selection ; orientation ; oviposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The alfalfa seed chalcid (ASC),Bruchophagus roddi, is a monophagous pest of alfalfa that parasitizes developing seeds. To further understand the olfactory basis of host-plant recognition by ASCs, we recorded electroantennograms (EAGs) from females and males to 39 volatiles from both alfalfa and red clover. The chemoreceptive sensitivity of ASCs was selective for certain general classes of compounds, defined by their carbon-chain length (C6 and C8), structure (aliphatics and phenolics), isomerism, and/or functional group (acetates, ketones, and alcohols). The compounds that elicited the largest EAGs were ranked as follows: (Z)-3-hexenyl acetate 〉 hexyl acetate ≥ acetophenone ≥ octan-3-one ≥ methyl salicylate 〉 octan-3-ol 〉 oct-1-en-3-one 〉 oct-1-en-3-ol ≥ (E)-β-ocimene ≥ (Z)- and (E)-3-hexen-1-ols. Over half the test compounds elicited significantly different responses between the sexes, and female antennal responses exceeded those of males for twice the number of these volatiles. Relationships of the tested volatiles to host-plant composition, EAG responses, and ASC behaviors showed no consistent correlations. However, nearly all of the host-plant volatiles known to stimulate behavioral activity also elicited moderate to potent EAG responses.
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  • 71
    ISSN: 1573-1561
    Keywords: Pheromone ; hydrocarbon ; diene ; (Z)-4-tridecenal ; oxidation ; Hymenoptera ; Braconidae ; Macrocentrus grandii ; parasitoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z)-4-Tridecenal was identified as a sex pheromone component ofMacrocentrus grandii, a larval parasitoid of the European corn borer. The aldehyde was found to be a common air oxidation product from a series of (Z,Z)-9,13-dienes of 27–33, 35, 37, 39, and 41 carbon atoms in female wasps. Synthetic (Z)-4-tridecenal and (Z,Z)-9,13-heptacosdiene each elicited flight initiation, upwind anemotaxis, casting, landing on the source, walking, wing fanning, and copulatory attempts by male wasps in a wind-tunnel. Field studies demonstrated that both compounds are synergized by a more polar component. Synthetic (E)-4-tridecenal was not attractive.
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  • 72
    ISSN: 1573-1561
    Keywords: Trichogramma brassicae ; Hymenoptera ; Trichogrammatidae ; kairomone ; pheromone ; Ostrinia nubilalis ; Lepidoptera ; Pyralidae biological control ; European corn borer ; parasitoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Volatile chemicals emanating from the different developmental stages ofOstrinia nubilalis Hübner (Lepidoptera, Pyralidae) increase the mobility ofTrichogramma brassicae Bezdenko (Hymenoptera, Trichogrammatidae) in a linear airflow olfactometer. In this paper, we have demonstrated that airborne chemicals from egg masses and virgin females during calling activity stimulate an intensive search behavior byTrichogramma females. On the other hand, emanations from mated females with extruded abdominal tips do not incite the parasitoid's movement. For the moment we cannot elucidate, with these bioassays, the real role of these kairomones as attractants, guides, stimulants, or retainers.
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  • 73
    ISSN: 1573-5117
    Keywords: mixing regime ; Laboratory Scale Enclosures ; phytoplankton ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photosynthesis of plankton sampled from the eutrophic Lake Loosdrecht was studied in Laboratory Scale Enclosures (LSEs) with regard to the rate of mixing. First, two LSEs were operated at different mixing rates. No significant differences in photosynthetic performance were found, with the exception of a depressed photosynthesis in the afternoon in the LSE which had a low mixing rate. Secondly, when mixing was stopped, the phytoplankton which stayed in the dark due to the steep light gradient in the LSE responded by changing its maximal photosynthetic capacity. The results show that the filamentous cyanobacteria in the lake can respond rapidly to changes in the depth of the mixed layer by altering their photosynthetic performance.
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  • 74
    ISSN: 1573-5079
    Keywords: Fe ; photosynthesis ; Photosystem II ; variable fluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It was found that DCMU had a differential effect at two concentration ranges on variable fluorescence kinetics in isolated chloroplasts. The increase in fluorescence rate at low concentrations of DCMU was abolished by preincubation of chloroplasts with ferricyanide or formate, treatments which were shown to convert Fe in the PS II reaction center (i.e., the FeQA complex) into a non-oxidizable form, but it was not affected by Tris treatment. Increase in fluorescence kinetics (at the initial linear rate) at high concentrations of DCMU was found to be abolished by Tris treatment but it was only marginally affected by ferricyanide or formate treatments. The effect of Tris could be abolished by addition of hydroquinone-ascorbate, which restored electron flow to the pool of secondary acceptors. Contrary to the effect of DCMU, no such differential concentration dependence of the variable fluorescence kinetics was found for atrazine. The increase in fluorescence kinetics (at the initial linear rate) at a low concentration rate of DCMU is presumably restricted to units which contain an oxidizable Fe in the FeQA complex. Increase in fluorescence kinetics (at the initial linear rate) at high DCMU concentration is probably related to the effect of DCMU at the QB site.
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  • 75
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    Photosynthesis research 32 (1992), S. 1-10 
    ISSN: 1573-5079
    Keywords: chlorophyll a/b ratio ; chlorophyll-proteins ; electron transport ; herbicide ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study was conducted to more clearly define the physiological effects of PS II herbicides on chloroplast thylakoid membrane activity and composition. Barley (Hordeum vulgare L. cv Boone) was grown in hydroponic culture at 20°C in a growth chamber with a light intensity of 500 μmole photons m-2 s-1. Atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine), a Photosystem II herbicide, was supplied continuously via the roots to 7-day-old plants. Atrazine concentrations greater than 0.07 ppm (0.32 μM) were associated with decreased leaf chlorophyll (chl), lowered chl a/b ratio, inhibition of chloroplast electron transport, and plant death within 1 to 2 weeks. Atrazine at 0.07 ppm was defined as sublethal because no toxic effects were observed. Sublethal atrazine induced a decrease in chl a/b ratio with no effect on leaf chl content. Photosynthetic electron transport was either unaffected in fully expanded leaves or slightly stimulated in expanding leaves by treatment of intact plants with 0.07 ppm atrazine. The major effect of sublethal atrazine was on the chl-protein complex composition. Sublethal atrazine increased the level of the Photosystem II light-harvesting complex (LHC-II) and lowered the level of the CP1a Photosystem I complex relative to controls. The numbers of Photosystem II and Photosystem I reaction centers and cytochrome b 6/f complexes per unit chl were not affected by sublethal atrazine. The overall result was an atrazine-induced redistribution of light-harvesting chl from Photosystem I to Photosystem II with no effect on the number of thylakoid membrane-protein complexes associated with electron transport.
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  • 76
    ISSN: 1573-5079
    Keywords: Cytochrome b-559 ; ADRY agent ; electron transport ; photosynthesis ; QA ; QB ; reaction center
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The possibility of a Photosystem II (PS II) cyclic electron flow via Cyt b-559 catalyzed by carbonylcyanide m-chlorophenylhydrazone (CCCP) was further examined by studying the effects of the PS II electron acceptor 2,6-dichloro-p-benzoquinone (DCBQ) on the light-induced changes of the redox states of Cyt b-559. Addition to barley thylakoids of micromolar concentrations of DCBQ completely inhibited the changes of the absorbance difference corresponding to the photoreduction of Cyt b-559 observed either in the presence of 10 μM ferricyanide or after Cyt b-559 photooxidation in the presence of 2 μM CCCP. In CCCP-treated thylakoids, the concentration of photooxidized Cyt b-559 decreased as the irradiance of actinic light increased from 2 to 80 W m-2 but remained close to the maximal concentration (0.53 photooxidized Cyt b-559 per photoactive Photosystem II) in the presence of 50 μM DCBQ. The stimulation of Cyt b-559 photooxidation in parallel with the inhibition of its photoreduction caused by DCBQ demonstrate that the extent of the light-induced changes of the redox state of Cyt b-559 in the presence of CCCP is determined by the difference between the rates of photooxidation and photoreduction of Cyt b-559 occuring simultaneously in a cyclic electron flow around PS II. We also observed that the Photosystem I electron acceptor methyl viologen (MV) at a concentration of 1 mM barely affected the rate and extent of the light-induced redox changes of Cyt b-559 in the presence of either FeCN or CCCP. Under similar experimental conditions, MV strongly quenched Chl-a fluorescence, suggesting that Cyt b-559 is reduced directly on the reducing side of Photosystem II.
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  • 77
    ISSN: 1573-5079
    Keywords: acetate ; bicarbonate effect ; CO2-depletion ; electron transport ; formate ; photosynthesis ; thermoluminescence ; Chlamydobotrys stellata ; Chlamydomonas reinhardtii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated the influence of CO2/HCO3 −-depletion and of the presence of acetate and formate on the in vivo photosynthetic electron transport in the two green algae Chlamydobotrys stellata and Chlamydomonas reinhardtii by means of thermoluminescence technique and mathematical glow curve analysis. The main effects of the removal of CO2 from the algal cultures was: (1) A shift of the glow curve peak position to lower temperatures resulting from a decrease of the B band and an increase of the Q band. (2) Treatment of CO2-deficient Chl. stellata with DCMU yielded two thermoluminescence bands in the Q band region peaking at around +12°C and +5°C; in case of Chl. reinhardtii DCMU treatment induced only one band with an emission maximum at +5°C. The presence of acetate or formate in CO2-depleted algal cultures lowered the intensities of all of the individual TL bands but that of a HT band (TL+37). The effects of CO2-depletion and of the presence of anions were fully reversible.
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  • 78
    ISSN: 1573-5079
    Keywords: electron transfer ; proton transfer ; photosynthesis ; quinone ; reaction center ; site-specific mutagenesis ; suppressor mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two acidic residues, L212Glu and L213Asp, in the QB binding sites of the photosynthetic reaction centers of Rhodobacter capsulatus and Rhodobacter sphaeroides are thought to play central roles in the transfer of protons to the quinone anion(s) generated by photoinduced electron transfer. We constructed the site-specific double mutant L212Ala-L213Ala in R. capsulatus, that is incapable of growth under photosynthetic conditions. A photocompetent derivative of that strain has been isolated that carries the original L212Ala-L213Ala double mutation and a second-site suppressor mutation at residue M43 (Asn→Asp), outside of the QB binding site, that is solely responsible for restoring the photosynthetic phenotype. The Asp,Asn combination of residues at the L213 and M43 positions is conserved in the five species of photosynthetic bacteria whose reaction center sequences are known. In R. capsulatus and R. sphaeroides, the pair is L213Asp-M43Asn. But, the reaction centers of Rhodopseudomonas viridis, Rhodospirillum rubrum and Chloroflexus aurantiacus reverse the combination to L213Asn-M43Asp. In this respect, the QB site of the suppressor strain resembles that of the latter three species in that it couples an uncharged residue at L213 with an acidic residue at M43. These reaction centers, in which L213 is an amide, must employ an alternative proton transfer pathway. The observation that the M43Asn→Asp mutation in R. capsulatus compensates for the loss of both acidic residues at L212 and L213 suggests that M43Asp is involved in a new proton transfer route in this species that resembles the one normally used in reaction centers of Rps. virddis, Rsp. rubrum and C. aurantiacus.
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  • 79
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    Plant cell, tissue and organ culture 31 (1992), S. 239-244 
    ISSN: 1573-5044
    Keywords: Vitis ; grapevine ; in vitro culture ; photomixotropy ; photosynthesis ; respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two Vitis species were cultured in vitro under photoautrophic (sucrose-free culture medium) and photomixotrophic (sucrose 15 g l-1) conditions during the period following microcutting rooting (day 34 to day 120). Several parameters were measured at the end of the culture: growth, plant dry weight, carbohydrate uptake from the medium and rates of photosynthesis and dark respiration. The two species behaved very differently. Under photoautotrophic conditions, dark respiration, net photosynthesis and daily CO2 fixation were higher in Vitis vinifera than in Vitis rupestris. Culture under mixotrophic conditions caused increase in growth, respiration and photosynthesis in Vitis rupestris. In contrast, photosynthesis decreased in Vitis vinifera under the same conditions.
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  • 80
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    Photosynthesis research 31 (1992), S. 157-164 
    ISSN: 1573-5079
    Keywords: Chlamydomonas reinhardtii ; chloroplast mutations ; enzyme catalysis ; photorespiration ; photosynthesis ; protein engineering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Temperature, activating metal ions, and amino-acid substitutions are known to influence the CO2/O2 specificity of the chloroplast enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase. However, an understanding of the physical basis for enzyme specificity has been elusive. We have shown that the temperature dependence of CO2/O2 specificity can be attributed to a difference between the free energies of activation for the carboxylation and oxygenation partial reactions. The reaction between the 2,3-enediolate of ribulose 1,5-bisphosphate and O2 has a higher free energy of activation than the corresponding reaction of this substrate with CO2. Thus, oxygenation is more responsive to temperature than carboxylation. We have proposed possible transition-state structures for the carboxylation and oxygenation partial reactions based upon the chemical natures of these two reactions within the active site. Electrostatic forces that stabilize the transition state of the carboxylation reaction will also inevitably stabilize the transition state of the oxygenation reaction, indicating that oxygenase activity may be unavoidable. Furthermore, the reduction in CO2/O2 specificity that is observed when activator Mg2+ is replaced by Mn2+ may be due to Mg2+ being more effective in neutralizing the negative charge of the carboxylation transition state, whereas Mn2+ is a transition-metal ion that can overcome the triplet character of O2 to promote the oxygenation reaction.
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  • 81
    ISSN: 1573-5079
    Keywords: photosynthesis ; 2-carboxyarabinitol 1-phosphate ; Fabaceae ; genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 2-carboxyarabinitol 1-phosphate (CA 1-P) is a naturally occurring inhibitor of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Members of the Fabaceae exhibit a particularly wide range in the extent of CA 1-P accumulation during darkness and include Phaseolus vulgaris, whose dark/light regulation of Rubisco activity is principally achieved by synthesis/degradation of CA 1-P. An extensive survey of the degree of dark inhibition of Rubisco was undertaken for the subfamily Papilionoideae to elucidate evolutionary patterns in the occurrence of this regulatory mechanism. Seventy-five species from 21 tribes were examined. Dark inhibition of Rubisco was found in ancestral tribes such as the Sophoreae, but was substantially reduced or absent in representative species of three more recently evolved tribes, Cicereae, Hedysareae and Vicieae. We conclude that regulation of Rubisco by CA 1-P is neither of recent origin nor of restricted distribution among the Papilionoideae. On the contrary, it becomes lost or less pronounced only in a minority of the more evolutionarily advanced species in this important subfamily.
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  • 82
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    Photosynthesis research 34 (1992), S. 409-418 
    ISSN: 1573-5079
    Keywords: PS I cyclic electron transport ; ferredoxin-plastoquinone reductase ; antimycin ; ferredoxin-NADP reductase ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Absorbance changes of ferredoxin measured at 463 nm in isolated thylakoids were shown to arise from the activity of the enzyme ferredoxin-plastoquinone reductase (FQR) in cyclic electron transport. Under anaerobic conditions in the presence of DCMU and an appropriate concentration of reduced ferredoxin, a light-induced absorbance decrease due to further reduction of Fd was assigned to the oxidation of the other components in the cyclic pathway, primarily plastoquinone. When the light was turned off, Fd was reoxidised and this gave a direct quantitative measurement of the rate of cyclic electron transport due to the activity of FQR. This activity was sensitive to the classical inhibitor of cyclic electron transport, antimycin, and also to J820 and DBMIB. Antimycin had no effect on Fd reduction although this was inhibited by stigmatellin. This provides further evidence that there is a quinone reduction site outside the cytochrome bf complex. The effect of inhibitors of ferredoxin-NADP+ reductase and experiments involving the modification of ferredoxin suggest that there may be some role for the reductase as a component of FQR. Contrary to expectations, NADPH2 inhibited FQR activity; ATP and ADP had no effect.
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  • 83
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    Photosynthesis research 33 (1992), S. 37-50 
    ISSN: 1573-5079
    Keywords: energy storage ; photosynthesis ; induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photoacoustic measurements of photosynthetic energy storage were conducted on water infiltrated pea and sugar maple leaves. The samples were vacuum infiltrated with pure water or with a suitable buffer. The use of such methodology permitted an accurate determination of the energy storage parameter at low modulation frequencies, where in non-infiltrated leaves oxygen evolution dominates the photoacoustic signal and does not allow energy storage measurements. Differences between infiltration media were not essential, however the use of pure water as infiltration medium sometimes caused instability of the measured energy storage, particularly at longer experimental time. Values of energy storage in individual samples ranged mostly between 0.2 to 0.35. Measured as a function of the modulation frequency, energy storage was found to be constant from about 10 to 200 Hz for pea leaves. In sugar maple leaves, the energy storage slightly increased between 100 and 500 Hz. Obtaining an accurate value for energy storage also allowed an accurate estimation of the O2 evolution contribution to the photoacoustic signal of an unfiltrated leaf. In a maple leaf its frequency dependence showed only the effect of diffusion in the entire frequency range (10–500 Hz). Energy storage transients were observed after long periods (ca. 1/4-2 hrs) of dark adaptation upon the transition to light. In this case the initial energy storage was roughly about 1/2 that of the steady state value indicating strong PS I activity, while PS II was transiently incompetent. Energy-storage increased during illumination in a way to correspond to photosynthetic induction events as previously measured by fluorescence and O2 evolution. Transients in energy storage were also found following high light to low light transitions (i.e., switch off of the saturating background light), that paralleled similar transients in oxygen evolution, showing initial transient inactivation followed by progressive reactivation of PS II.
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  • 84
    ISSN: 1573-5079
    Keywords: chlorophyll fluorescence ; quantum yield ; quenching ; photosynthesis ; Photosystem II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We tested the two empirical models of the relationship between chlorophyll fluorescence and photosynthesis, previously published by Weis E and Berry JA 1987 (Biochim Biophys Acta 894: 198–208) and Genty B et al. 1989 (Biochim Biophys Acta 990: 87–92). These were applied to data from different species representing different states of light acclimation, to species with C3 or C4 photosynthesis, and to wild-type and a chlorophyll b-less chlorina mutant of barley. Photosynthesis measured as CO2-saturated O2 evolution and modulated fluorescence were simultaneously monitored over a range of photon flux densities. The quantum yields of O2 evolution (ØO2) were based on absorbed photons, and the fluorescence parameters for photochemical (qp) and non-photochemical (qN) quenching, as well as the ratio of variable fluorescence to maximum fluorescence during steady-state illumination (F'v/F'm), were determined. In accordance with the Weis and Berry model, most plants studied exhibited an approximately linear relationship between ØO2/qp (i.e., the yield of O2 evolution by open Photosystem II reaction centres) and qN, except for wild-type barley that showed a non-linear relationship. In contrast to the linear relationship reported by Genty et al. for qp×F'v/F'm (i.e., the quantum yield of Photosystem II electron transport) and ØCO2, we found a non-linear relationship between qp×F'v/F'm and ØO2 for all plants, except for the chlorina mutant of barley, which showed a largely linear relationship. The curvilinearity of wild-type barley deviated somewhat from that of other species tested. The non-linear part of the relationship was confined to low, limiting photon flux densities, whereas at higher light levels the relationship was linear. Photoinhibition did not change the overall shape of the relationship between qp×F'v/F'm and ØO2 except that the maximum values of the quantum yields of Photosystem II electron transport and photosynthetic O2 evolution decreased in proportion to the degree of photoinhibition. This implies that the quantum yield of Photosystem II electron transport under high light conditions may be similar for photoinhibited and non-inhibited plants. Based on our experimental results and theoretical analyses of photochemical and non-photochemical fluoresce quenching processes, we conclude that both models, although not universal for all plants, provide useful means for the prediction of photosynthesis from fluorescence parameters. However, we also discuss that conditions which alter one or more of the rate constants that determine the various fluorescence parameters, as well as differential light penetration in assays for oxygen evolution and fluorescence emission, may have direct effect on the relationships of the two models.
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  • 85
    ISSN: 1572-9834
    Keywords: marsh deterioration ; photosynthesis ; productivity ; salt marsh ; sediment addition ; Spartina alterniflora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transpiration, leaf conductance, net photosynthesis, leaf growth, above-ground biomass and regeneration of new culms were studied in a rapidly subsiding Spartina alterniflora Lois. salt marsh following the addition at 47 and 94 Kg m−2 of new sediment. Plant growth was enhanced in response to sediment addition as was evident by a significant increase in leaf area, above-ground biomass production and regeneration of new culms (p ≤ 0.05). Leaf conductance and transpiration rates were significantly greater in sediment treated plants than in control plants (p ≤ 0.05). Enhanced production of culms per unit area of marsh resulted in increased leaf area which allowed a greater capacity for net photosynthesis and contributed to increases in above-ground biomass of sediment treated plots.
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  • 86
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    Journal of chemical ecology 18 (1992), S. 1991-2002 
    ISSN: 1573-1561
    Keywords: Ants ; defense ; phytophage ; predation ; sulfur allelochemicals ; Allium ; Formica fusca ; Formica selysi ; Hymenoptera ; Formicidae ; Acrole-piopsis assectella ; Lepidoptera ; Acrolepiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract It has been shown previously that sulfur volatiles produced byAllium plants affect the behavior of their specialist phytophages and of their specialist entomophages. The action of these compounds in protecting the leek mothAcrolepiopsis assectella against generalist entomophages was studied in comparison to the proposed original defensive role of these compounds against generalist herbivorous insects. Two ants species,Formica selysi andF. fusca, were used as generalist predators. Six behavioral criteria of the predatory behavior of the ants were studied in presence of the last-instar caterpillars (C5). C5 reared on artificial diets with or without leek components were tested, as well as C5 soaked in frass of leek-reared caterpillars or disulfide solutions. In addition, the response of the ants to pure chemicals found in leek was studied using honey solutions with or without sulfur compounds. The sulfur allelochemicals ofAllium plants have a negative action on predatory ants. Interestingly, the nonvolatile precursors of sulfur volatiles ofAllium plants seem to have a protective role for their phytophagous insects against generalist entomophages.
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  • 87
    ISSN: 1573-1561
    Keywords: Diaprepes abbreviatus ; Coleoptera ; Curculionidae ; defensive secretion ; Solenopsis geminata ; Hymenoptera ; Formicidae ; sesquiterpenes ; 5-methyl-8-isopropenyl-3 ; 4,4a,5,6,7,8,8a- octahydro-2-naphthalene carboxy aldehyde
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Since several species of predatory ants show some kind of repulsion towards the first-instar larvae (FIL) ofDiaprepes abbreviatus L., the predatory behavior ofSolenopsis geminata (F.), a common ant in the citrus groves in Guadeloupe, was studied. Different extracts of larvae were disposed on egg masses ofD. abbreviatus and presented as prey to the ants, both in the field and in the laboratory. The ants are repelled by the FIL extracts. The allelochemicals involved are produced in large amounts, from 5 to 20 ng per larva. Physiochemical analyses have led to the identification of two sesquiterpenes of molecular weight 218 and 234, secreted in the respective proportions of 65 and 35%.
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  • 88
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    Journal of chemical ecology 18 (1992), S. 1633-1640 
    ISSN: 1573-1561
    Keywords: Apis mellifera ; honeybee ; Hymenoptera ; Apidae ; queen ; fecal ; pheromones ; esters ; kin recognition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Previous work has shown that queen honeybees,Apis mellifera, produce waxy esters composed of 8–14 carbon acids and 6–14 carbon alcohols in their feces. We tested these esters for effects on nestmate recognition; 11 of the 12 esters tested significantly modified the recognition characteristics of worker honeybees. Pairwise tests showed that workers can discriminate between at least some pairs of queen esters and that workers can discriminate between a queen ester and hexadecane (another known nestmate recognition cue). These results suggest that a queen may use the esters to enable workers to recognize her or to scent-mark her colony.
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  • 89
    ISSN: 1573-8221
    Keywords: histamine ; lipid peroxidation ; sensitization ; antioxidant system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 90
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    Bulletin of experimental biology and medicine 114 (1992), S. 1239-1241 
    ISSN: 1573-8221
    Keywords: experimental diabetes ; glycosylated hemoglobin ; lipid peroxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 91
    ISSN: 1573-8221
    Keywords: opioid neuropeptides ; lipid peroxidation ; prostaglandins ; heart
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 92
    ISSN: 1573-8221
    Keywords: emotional stress ; open field ; lipid peroxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 93
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    Bulletin of experimental biology and medicine 113 (1992), S. 170-172 
    ISSN: 1573-8221
    Keywords: liposomes ; lipid peroxidation ; thymocytes ; radiosensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 94
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    Bulletin of experimental biology and medicine 113 (1992), S. 181-183 
    ISSN: 1573-8221
    Keywords: lipid peroxidation ; atherosclerosis ; lipoproteins ; liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 95
    Electronic Resource
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    Bulletin of experimental biology and medicine 113 (1992), S. 153-155 
    ISSN: 1573-8221
    Keywords: iron ; circadian rhythms ; excretion ; lipid peroxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 96
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    Bulletin of experimental biology and medicine 113 (1992), S. 489-492 
    ISSN: 1573-8221
    Keywords: myocardial ischemia ; pentothenic acid derivatives ; lipid peroxidation ; lactate ; ATP ; creatine phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 97
    ISSN: 1573-8221
    Keywords: hyperbaric environment ; lipid peroxidation ; membrane microviscosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 98
    Electronic Resource
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    Bulletin of experimental biology and medicine 113 (1992), S. 641-644 
    ISSN: 1573-8221
    Keywords: chemiluminescence ; lipid peroxidation ; antioxidants ; copper(II) complexes ; coumarin ligands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 99
    ISSN: 1573-8221
    Keywords: conditioned reflex ; adrenergic cardionecrosogenic stress ; verapamil ; superoxide dismutase ; lipid peroxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 100
    Electronic Resource
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    Bulletin of experimental biology and medicine 113 (1992), S. 653-655 
    ISSN: 1573-8221
    Keywords: free radicals ; lipid peroxidation ; emotional stress ; rat hypothalamus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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