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  • Articles  (33)
  • 42.55  (25)
  • Chemical Engineering
  • Electronic structure and strongly correlated systems
  • Saccharomyces cerevisiae
  • Springer  (33)
  • 1980-1984  (33)
  • 1980  (33)
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  • Articles  (33)
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  • 1980-1984  (33)
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  • 1
    ISSN: 1432-0983
    Keywords: Gene cloning ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have carried out experiments aimed at explaining the observed variations in transformation frequencies when Saccharomyces cerevisiae or Saccharomyces carlbergensis are transformed with chimeric plasmids that contain one of 4 possible EcoRI fragments of the yeast 2-μm circle. These plasmids fall into 2 classes when used to transform 2 different yeast his3 auxotrophs, one (strain LL20) harbours indigenous 2-μm circle, and the other (strain YF233) is devoid of this plasmid. Hybrid plasmids containing either the 2.4 mega-dalton (mD) R-form EcoRI fragment (pYF88) or the l.4 mD L-form EcoRI fragment (pYF177) of 2-μm circle transform either of the two hosts at a high frequency (50,000 colonies per Mg in LL20 and 10,000 colonies per μg in YF233). Hybrid plasmids containing the 1.5 mD R-form EcoRI fragment (pYF87) or the 2.5 mD L-form EcoRI fragment (pYF178) of the 2-μm circle transform LL20 at a reduced frequency (6,000–16,000 colonies per μg) and YF233 at extremely low frequencies (1–5 colonies per μg). All plasmids retrieved from strain YF233 that had been transformed with pYF88 or pYF177 were identical to the original transforming plasmid. Of the plasmids retrieved from strain LL20 that had been transformed with pYF87 and pYF178, approximately half had acquired an extra copy of the 2-μm circle. Of the plasmids retrieved from strain LL20 that had been transformed with pYF88 and pYF177, an average of only approximately 13% had acquired an extra copy of 2-μm circle. Taken together, these observations indicate that the transformation of yeast by a plasmid lacking the ability to replicate (pYF87 and pYF1780) occurs by the recombinational acquisition of 1 copy of the host 2-μm circle, which serves to supply the incoming plasmid with missing essential sequences. A comparison of 2-μm circle DNA fragments carried by pYF88 and pYF177 indicates that the region of 2-μm circle required for high frequency transformation is a 1.2 mD segment that is common to the 2.4 mD R-form and 1.4 ml) L-form EcoRI fragments. This region extends from the EcoRI cut site adjacent to the PstI site, through to the end of the inverted repeat. However, the inverted repeat sequence alone is not sufficient to bestow high frequency transformation of yeast.
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  • 2
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    Current genetics 2 (1980), S. 115-120 
    ISSN: 1432-0983
    Keywords: Galactose fermentation ; Saccharomyces cerevisiae ; Regulatory mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A novel type of regulatory mutation for galactose metabolism in Saccharomyces cerevisiae is described. The mutation named gal11 was recessive, non-allelic to GAL4, GAL80, GAL2, or GAL3, and unlinked to the gene cluster of GAL1, GAL10, and GAL7. It caused a ‘coordinate’ reduction of galactokinase, galactose-1-P uridylyl transferase, and UDP-glucose 4-epimerase by a factor of more than 5, rendering the mutant cells galactose-nonfermenting. The effect of the mutation was manifested not only in cells grown on galactose but also in cells constitutively synthesizing the galactose-metabolizing enzymes.
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  • 3
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    Current genetics 2 (1980), S. 223-228 
    ISSN: 1432-0983
    Keywords: Transcriptional Units ; GAL Genes ; Saccharomyces cerevisiae ; UV mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The size of the transcriptional unit of the structural genes for three galactose-metabolizing enzymes which form a cluster on chromosome II in Saccharomyces cerevisiae was studied by the ultraviolet light (UV)-mapping technique. Thus the size of the primary transcripts of GAL7 for galactose-1-phosphate uridylyl transferase, GAL10 for uridine diphosphoglucose 4-epimerase, or GAL1 for galactokinase were estimated to be 0.81 x 106, 1.1 x 106, or 1.3 x 106 respectively. In the light of these data together with the known directions of transcription of the genes, we concluded that each of three genes was transcribed from its own promoter.
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  • 4
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    Applied physics 22 (1980), S. 175-184 
    ISSN: 1432-0630
    Keywords: 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The problem of output-coupling optimization of a transversely or longitudinally pumped dye laser is treated in terms of a rate equation approach under steady state considerations. The theoretical study considers the spatial variation of the gain distribution and such typical features of a dye laser as reabsorption, triplet effects and excited state absorption. Numerical results obtained with realistic values of typical laser parameters (cross-sections for stimulated emission and reabsorption, number density of active molecules etc.) indicate that a higher degree of output coupling and consequently higher output powers are feasible upon a complete consideration of the spatial gain distribution
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  • 5
    ISSN: 1432-0630
    Keywords: 42.55 ; 42.80 ; 33
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract As an outcome of a systematic study of excitation kinetics and fluorescence spectra concerning theD′(3π2u )→A′(3π2g ) transitions in homonuclear diatomic halogens and corresponding transitions in interhalogen molecules [1], this paper describes, for the first time, TEA laser action in the three halogen monofluorides ClF, BrF, and IF. Output energies in the mJ range were demonstrated in a fast TEA discharge arrangement at the wavelengths of 284.4, 354.5, and 490.8 nm for ClF, BrF, and IF, respectively. The results suggest parameter scalability and wavelength tunability.
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  • 6
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    Applied physics 21 (1980), S. 25-28 
    ISSN: 1432-0630
    Keywords: 35 ; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract He−Zn and He−ZnCl2 hollow cathode discharges were compared. The metal vapour concentration necessary for laser operation can be achieved at a lower temperature for the molecular substance. On the other hand, the efficiency decreases because part of the He ions necessary for the selective excitation of the upper laser level of the metal ion are lost in the dissociation process.
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  • 7
    ISSN: 1432-0630
    Keywords: 07.65 ; 42.55 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The application of the high-pressure chamber with a pressure transmitting medium (liquid at room temperature) to diode-laser tuning was found to be a useful method for high-resolution spectroscopy. Ammonia absorbtion spectra in the 10 μm region were recorded with a resolution approaching the Doppler limit.
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  • 8
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    Applied physics 21 (1980), S. 237-240 
    ISSN: 1432-0630
    Keywords: 42.55 ; 42.60 ; 34.50
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Selective single-line oscillation has been obtained from an NH3 laser in the range from 11 to 13 μm. Pumping of high pressure NH3−He mixture with theR(30) line of a TEA-CO2 laser made it possible to tune on more than 30 lines with the aid of a grating. However, such line tunable oscillation was not observed when theR(16) line was used for pumping. Reasons for this difference are discussed.
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  • 9
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    Applied physics 21 (1980), S. 319-321 
    ISSN: 1432-0630
    Keywords: 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We report the observation that the helical TE N2 laser can effectively be simplified by giving up the use of decoupling elements as well as by abolishing the segmentation of the electrode structure. Although, as a consequence of this simplification, the operating pressure range was slightly decreased, the output power could be improved by roughly 30%, a result which is attributed to the new electrode geometry exhibiting lower inductance and lower damping losses.
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  • 10
    ISSN: 1432-0630
    Keywords: 87 ; 42.55 ; 82.50
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We report on first attempts for selective action on nucleic acid components, namely uracil and adenine, using picosecond light pulses. Selective action was achieved both in the case of irradiation of mixtures of bases and in the case of irradiation of mixtures of different nucleic acids components, which contain uracil and adenine.
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  • 11
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    Applied physics 22 (1980), S. 213-218 
    ISSN: 1432-0630
    Keywords: 42.55 ; 42.60 ; 42.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We present periodic solutions, which are described in terms of Jacobian elliptic functions, for the steady state pulses from a laser system consisting of a pair of amplifiers of unequal relaxation time mode locked by a “fast” saturable absorber. The pulsewidth and the average power in the pulse are expressed interms of the system parameters through two different approaches, where detailed plots are given for one of the approaches. Summary of the characters to be possessed by the amplifier pair to ensure the reliable production of shortest possible pulses is given.
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  • 12
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    Applied physics 22 (1980), S. 277-281 
    ISSN: 1432-0630
    Keywords: 42.55 ; 52
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract An analytical model is applied to the concept of a solar-pumped MHD laser utilizing the CsXe excimer and Cs2 dimerA-X laser transitions. The model predicts practical gain coefficients on the excimer band for supersonic flow of a 2000 K, 20 atmosphere cesium-xenon plasma across a ∼10 kG magnetic field.
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  • 13
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    Applied physics 22 (1980), S. 421-427 
    ISSN: 1432-0630
    Keywords: 42.55 ; 47 ; 52
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The operation of an electrically excited gasdynamic CO laser with closed-cycle gas flow is described. Details of the gasdynamics, the recompression, and the cooling of the flowing gas are given. The electrical discharge produces contaminating gas compounds—in particular CO2—which accumulate in the recirculating gas. The resulting transient behavior of the laser gas composition, the discharge characteristics, and the laser performance are studied and compared with the results obtained with the same CO laser in an open-flow system. It is found that the closed-cycle system may be operated for considerable time if the CO2 contamination is removed from the laser gas.
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  • 14
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    Applied physics 23 (1980), S. 15-19 
    ISSN: 1432-0630
    Keywords: 64.75+g ; 61.70T ; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The solubility of several dopants (Sb, Ga, Bi, In) in laser treated silicon has been investigated. The dopants were introduced by vacuum deposition followed by ruby laser irradiation. Their solubility was determined by Rutherford backscattering spectroscopy measurements in channelling and random conditions. In all cases, a maximum solubilityC S * , much higher than the equilibrium solubility limitC S 0 and independent of the pulsed laser energy density, was found. The values obtained are in good agreement with those calculated from a simple model based on phase diagram considerations, using the relationship: $$C_S^* = \frac{{C_S^0 }}{{k_0 }}k^* ,$$ wherek 0 andk * are the equilibrium and effective distribution coefficients. Finally, the existence of a new solubility limit for a laser treatment is discussed.
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  • 15
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    Applied physics 23 (1980), S. 127-130 
    ISSN: 1432-0630
    Keywords: 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Two new cw submillimetre lines optically pumped by the 9 μR(30) CO2 laser have been observed for14NH3 and assigned to thea(6,0)→s(5,0) transition and to its cascade transitions(5,0)→a(4,0) in thev 2=1 state. In addition, six new emission lines have been observed for NH2D, and one tentatively assigned to the (11,1)→(10,1) transition of ND3. Methylamine has also been investigated and twenty-eight new emission lines have been discovered.
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  • 16
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    Applied physics 23 (1980), S. 341-344 
    ISSN: 1432-0630
    Keywords: 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract An N2-laser of 19 ns pulse-duration and an energy of 30 mJ has been developed and examined. A dc preionization, a grooved electrode and a partial electrical screening of the discharge tube permit reproducible single-shot operation and yield a homogeneous beam.
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  • 17
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    Applied physics 21 (1980), S. 407-409 
    ISSN: 1432-0630
    Keywords: 42.55 ; 52 ; 85.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A method is described for detection of CO2-laser power variations which requires no optical detector. It is based on plasma-tube impedance changes induced by the laser power flow within the cavity. A constant-current high-voltage power supply and a special equalizer are necessary to obtain a voltage proportional to the laser power. Details for the design of the equalizer are given. The detection scheme works for frequencies up to about 5 kHz and can be used for laser frequency stabilization or signature analysis.
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  • 18
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    Applied physics 22 (1980), S. 149-154 
    ISSN: 1432-0630
    Keywords: 42.55 ; 52
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The stability of parallel pin electrode CO2-laser discharges, coupled by carrier diffusion only, against concentration of current in one column and subsequent transition into an arc, is investigated theoretically. A small signal description of the time dependent behaviour of a column with falling characteristic caused by a wall sheath of considerable width is used. It is shown that an inductance in series to each discharge is not able to avoid glow to arc transition, though enlarges the duration of the glow.
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  • 19
    ISSN: 1432-0630
    Keywords: 33 ; 82.50 ; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Absorption and fluorescence spectra of rarefied vapors ofp-terphenyl, 1,4-bis-[2(5-phenyloxazolyl)]-benzene, 2,5-diphenyl-1,3,4-oxadiazole, 2,5-diphenyl-1,3-oxazole, 2,5-diphenylfuran are measured. Extinction coefficients, quantum yields of fluorescence and one-photon decomposition of molecules are determined for vapors excited by pulsed laser radiation at 266 and 355 nm in the temperature range from 200 to 400°C. It is shown that for all cases studied the one-photon decomposition is only a small part of the total quantum yield of the radiationless deactivation of the fluorescent state. Laser action has, for the first time, been obtained in 2,5-diphenylfuran vapor at 356 nm.
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  • 20
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    Applied physics 22 (1980), S. 171-174 
    ISSN: 1432-0630
    Keywords: 42.55 ; 82.50
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A previous analysis that described loss of gain in a dye solution as a result of photochemical decomposition by pump photons is refined and is extended to the situation where impurities absorb at the pump wavelength. A limitation in the first paper is that it described only a single absorption depth in the medium at a constant pump fluxI p. The present examination includes the fall-off ofI p and integrates the total signal output with depth for a single-pass amplifier operated in the fully saturated gain condition. The equations are simpler, but as before loss of output, expressed in terms of a quantum yield of laser decayQ L, is minimized for a photochemically bleachable dye, and for one with high gain. Pumping in the vicinity of the absorption maximum is also advantageous.
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  • 21
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    Applied physics 22 (1980), S. 355-359 
    ISSN: 1432-0630
    Keywords: 32 ; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Fluorescence spectra in the near infrared are observed of the dye Eastman 9860 and of new, faster mode-locking dyes. The quantum efficiencies are determined to be small with η≃10−3. The short fluorescence lifetimes are deduced from optical gain measurements. The fluorescence time-constants are found to have the same value as the corresponding absorption recovery times. Even laser action is observed in these fast saturable absorbers previously belieyed to be non-fluorescent.
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  • 22
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    Applied physics 23 (1980), S. 135-140 
    ISSN: 1432-0630
    Keywords: 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A laser system capable of generating picosecond pulses of 2 ps duration and peak powers of up to 50 MW, tunable throughout the emission spectrum of the lasing medium is described. The system consists of a cw dye laser which is tuned and is actively mode-locked by means of a novel interferometer. Single-pulse amplification is achieved via a two-stage N2-laser-pumped amplifier arrangement. One of the most attractive features of the system here described is its inherent versatile broad tunability, which should allow, with different lasing media and pump sources, ps pulse generation from 400 to 700 nm using the same interferometer device.
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  • 23
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    Applied physics 23 (1980), S. 295-298 
    ISSN: 1432-0630
    Keywords: 41 ; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract An analysis of stimulated emission of radiation by an electron beam passing through a periodic longitudinal electrostatic field is presented, and its use in a free-electron laser structure is discussed. The analysis is based on a coupled-mode calculation of the interaction between a waveguided electromagnetic mode and an electron-beam plasma with finite cross section. The dispersion equation derived applies to the collective as well as the single electron regime.
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  • 24
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    Applied physics 21 (1980), S. 95-114 
    ISSN: 1432-0630
    Keywords: 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The development of excimers and excimer lasers are reviewed. The excimers of the noble gases (Xe 2 * , Kr 2 * , Ar 2 * ) and of the noble gas halides (e.g. KrF, XeCl) which, respectively, radiate in the vacuum ultra-violet and ultra-violet regions of the spectrum are described in terms of their structure, spectroscopy and formation kinetics and the methods of pumping, operational characteristics and applications of the lasers are discussed.
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  • 25
    ISSN: 1432-0630
    Keywords: 35 ; 42.55 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A saturated absorption technique, using an actively stabilized cw dye laser has been developed to measure the saturation intensities of hyperfine transitions in a gaseous medium. Hyperfine lines belonging to theB-X system of molecular iodine have been investigated. The experimental method proposed, provides a means for measurement of the absolute value of the saturation parameter of the transition with an accuracy better than 10%. The behavior of the saturation intensity of some transitions with iodine pressure has been investigated in the range 30 m Torr→90mTorr.
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  • 26
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    Applied physics 22 (1980), S. 219-225 
    ISSN: 1432-0630
    Keywords: 42.55
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A simple single particle model of a free-electron laser (FEL) amplifier has been used in a computer simulation to determine the maximum fractional conversion of electron kinetic energy to laser energy. The simulation results can be represented by a single universal curve. A simple scaling relationship for the length of the optimized constant period helix together with the universal curve permit one to predict maximum fractional energy conversion for any set of values of initial electron energy, initial laser intensity, magnetic field amplitude, and magnet period.
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  • 27
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    Applied physics 23 (1980), S. 299-302 
    ISSN: 1432-0630
    Keywords: 42.30 ; 42.55 ; 42.65
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The spatial coherence function |Γ(s)| of ruby laser pulses has been measured by self-diffraction at a laser-induced grating depending on the distances between two points in the beam profile. The spatial coherence of the laser beam was changed by operating the laser in the fundamental transverse mode, in a transverse multimode and by applying different pump powers. The degree of spatial coherence was experimentally found to be a Gaussian, as described by the Collett-Wolf model. The new method for measuring the spatial coherence function |Γ(s)| is described and possible improvements are discussed.
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  • 28
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    Applied physics 23 (1980), S. 345-348 
    ISSN: 1432-0630
    Keywords: 42.55
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract New FIR emissions lines have been observed for HCOOH, D2CO and CD3Br. Assignments have been made of the HCOOH lines and for most of those of CD3Br. For D2CO, most of the new emissions showed a corresponding cascade transition.
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  • 29
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    Archives of microbiology 128 (1980), S. 157-161 
    ISSN: 1432-072X
    Keywords: Lactobacillus brevis ; Streptococcus lactis ; Saccharomyces cerevisiae ; Concanavalin A ; Symbiosis ; Tibi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tibi grains consist of a unique and very stable symbiotic association of Lactobacillus brevis, Streptococcus lactis and Saccharomyces cerevisiae embedded in a dextran matrix. The structural organization of the grain was examined by light, scanning and transmission electron microscopy. The grain was constituted of an outer compact layer and an inner spongy structure. The outer layer was more densely populated by the microorganisms than the inner layer but dextran, stained on frozen thin sections with fluorescein-conjugated concanavalin A, was more abundant in the inner layer.
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  • 30
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    Archives of microbiology 125 (1980), S. 89-95 
    ISSN: 1432-072X
    Keywords: Sulfur dioxide ; Sulfite ; Air polluting substances ; Saccharomyces cerevisiae ; Active agent of irreversible cell inactivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract After a short period of tolerance, living cells of Saccharomyces cerevisiae were irreversibly damaged by low concentrations of sulfite. The length of the period of tolerance and the rate of the damaging effect depended on the concentration on sulfite, pH-value, temperature, the physiological state of the cells, and incubation time. Inhibitors of protein synthesis and mitochondrial ATP synthesis did not alter the deleterious effect of sulfite on living cells. Furthermore, cell damage leading to inhibition of colony formation occured under aerobic as well as under anaerobic conditions. Prior to cell inactivation sulfite induced the formation of respiratory deficient cells. The active agent was shown to be SO2.
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  • 31
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Catabolite repression and inactivation ; Inhibition of protease B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Catabolite inactivation of fructose-1,6-bisphosphatase, isocitrate lyase, phosphoenolpruvate carboxykinase and malate dehydrogenase in intact cells could be prevented by phenylmethylsulfonyl fluoride added 40 min prior to the addition of glucose. Protein synthesis, fermentative and respiratory activity and catabolite repression were not affected. Elimination of catabolite inactivation by the addition of PMSF revealed that catabolite repression started at different times for different enzyme.
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  • 32
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    Archives of microbiology 124 (1980), S. 285-287 
    ISSN: 1432-072X
    Keywords: Yeast ; Saccharomyces cerevisiae ; Heat killing ; Membrane damage ; Genetic damage ; Growth temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The resistance of exponentially growing yeast cells to killing by exposure to 52°C increased markedly as the growth temperature was increased. Identical killing curves were obtained for cells suspended in growth medium or in 0.9% saline. Cells resistant to killing at 52°C were quite sensitive to killing at slightly higher temperatures. These results suggest a primary role for membrane damage in the mechanism of heat killing.
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  • 33
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    Plant and soil 57 (1980), S. 41-47 
    ISSN: 1573-5036
    Keywords: Amino acids ; Nucleic acid bases ; Saccharomyces cerevisiae ; Vitamins ; Yeast autolyzate ; Yield stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effect of yeast autolyzate on the growth of vineless pea plant was investigated. The yield was increased remarkably when yeast autolyzate was added, as compared with the control area in which the plants received only mineral fertilizer solution. The yield stimulation was the highest where 0.1 ppm of the autolyzate was added, the yield increasing by more than 80%. The reason why the yields were lower in the areas where the amount of yeast autolyzate added was incrased to 1 ppm and to 10 ppm, was presumed to be due to the fact that the substances may have been absorbed, decomposed, and utilized by soil microorganisms.
    Type of Medium: Electronic Resource
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