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  • General Chemistry  (865)
  • Organic Chemistry  (695)
  • Cell & Developmental Biology  (192)
  • Physical Chemistry
  • Wiley-Blackwell  (1,752)
  • 1980-1984
  • 1970-1974  (1,752)
  • 1970  (1,752)
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  • Wiley-Blackwell  (1,752)
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  • 1980-1984
  • 1970-1974  (1,752)
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  • 101
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    Enzyme kinetics in mammalian cells. II. Simultaneous determination of rate constants for the first three steps of galactose metabolism in red cellsFrom the Eleanor Roosevelt Institute for Cancer Research and the Department of Biophysics (contribution 384), University of Colorado Medical Center, Denver, Colorado. This investigation was supported by U.S. Public Health Service grant 5 PO1 HD02080 from the National Institute of Child Health and Human Development and by the American Cancer Society grant T-482. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 49-56 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The method for simultaneous measurement of the first two sequential enzymes of galactose metabolism of red cells has been extended to include the third step of the chain, epimerization of uridine diphosphogalactose to uridine diphosphoglucose. The method is rapid and precise and is applicable to a variety of genetic-biochemical problems.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/jcp.1040750106
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  • 102
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    An improved chemically defined culture medium for strain L mouse cells based on growth responses to graded levels of nutrients including iron and zinc ions (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 65-72 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Nutritional factors were evaluated for effects on growth of mouse fibroblast cells in suspension in a chemically defined medium. Quantitative requirements for each of the essential amino acids, choline, inorganic phosphate, iron, and zinc were established. An improved chemically defined medium was formulated on the basis of the findings yielding populations of L cells in excess of 5 × 106 per ml without nutrient replenishment. When spent medium was replaced periodically, yields approaching 30 × 106 cells per ml were attained. The efficiency of utilization of most amino acids in the new medium appears to be 2- to 3-fold better than results reported by others.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/jcp.1040750108
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  • 103
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    Nitrogen metabolism during synchronous growth of chlorella. II. Free-, peptide-, and protein-amino acid distributionThis paper represents part of the dissertation submitted by the senior author in partial fulfillment of the requirements of Doctor of Philosophy. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 73-82 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The levels of free-, peptide-, and protein-amino acids were measured during the synchronous growth and division cycle of a thermophilic strain of Chlorella pyrenoidosa. Most of the protein amino acids exhibited little periodism (as % of total cellular-N); however, the free- and peptide-amino acids showed a variety of dramatic changes in level during the cell cycle.Fractionation of the acid-soluble peptides by Sephadex gel-filtration showed that an average of only 2.8% of the peptide amino acids were associated with peptides of high molecular weight (〉 5000), while approximately 75% of the peptide amino acids were components of low molecular weight peptides (〈 700). The low molecular weight peptides were predominately made up of relatively few amino acids (i.e., alanine, glutamate, lysine, glycine and arginine accounted for approximately 92% of the low molecular weight peptide amino acids).Several experiments revealed that nucleotide-peptides do not contribute significantly to the pool of acid-soluble peptides during the cell cycle of this organism.
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    URL: http://dx.doi.org/10.1002/jcp.1040750109
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  • 104
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    Nuclear alterations in mammalian cells induced by L-canavanineSupported by: PHS grants CA-07239 and CA-05206 and Research Career Development Award No. CA-5K3-CA-13, 959. I wish to thank Miss Shirley Anderson for the excellent electronmicroscopy and cell culture work. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 129-131 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Mammalian cells treated four hours with the L-arginine analogue, L-canavanine (Cana), develop alterations in nuclear architecture characterized by the formation of irregular aggregates of nucleoplasmic contents, frequently attached to the nuclear membrane. On the basis of previous studies showing that Cana is incorporated into nuclear proteins and inhibits DNA synthesis over similar periods of exposure, it is postulated that the formation of canavanyl protein-DNA aggregates interfere with DNA replication.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/jcp.1040750115
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  • 105
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    Colony-forming cells in organ cultures of embryonal liver (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 163-165 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Livers from 17- to 20-day CBA mouse embryos were maintained for three weeks in organ culture. During this period, hematopoiesis continued; morphologically recognizable cells were identified until day 24 and hematopoietic cells with colony forming ability were present until day 23. The method appears to hold promise for studies of hematopoietic differentiation in vitro.
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    URL: http://dx.doi.org/10.1002/jcp.1040750205
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  • 106
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    Density distribution analysis of In vivo and In vitro colony forming cells in bone marrowPublication 1350 from The Walter and Eliza Hall Institute.This work was supported by grants from the National Health and Medical Research Council, Canberra, and the Australian Research Grants Committee. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 167-179 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The technique of buoyant density separation in gradients of Bovine Serum Albumin has been used to separate hemopoietic cell populations in mouse bone marrow that form in vivo spleen colonies and in vitro colonies of granulocytes and macrophages in an agar culture system. The density distribution profiles showed a number of reproducible density subpopulations of both in vivo and in vitro colony forming cells (C.F.C.'s). The mean density of in vitro C.F.C.'s exceeded that of the in vivo but overlap of the density profiles of the two populations was evident. Density-related differences in seeding efficiency of in vivo C.F.C.'s were observed.Freund's adjuvant treatment increased marrow and spleen in vitro C.F.C. populations. Marrow density profiles obtained three and seven days after adjuvant showed a progressive increase in in vitro C.F.C.'s in a restricted density region with no associated elevation of in vivo activity.The antimitotic agent, vinblastine, revealed differences in mitotic activity between the two cell populations, reducing the in vitro C.F.C. population to .07% and the in vivo to 5% of normal in 24 hours. Density separation of vinblastine-treated marrow produced density regions devoid of in vitro activity but containing in vivo in vivo C.F.C.'s which, upon transfer to irradiated recipients, regenerated both in vivo and in vitro density distribution profiles.
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/jcp.1040750206
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  • 107
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    Differential proliferation of erythroid and granuloid spleen colonies following sublethal irradiation of the bone marrow donorThis investigation was supported by United States Public Health Service grants CA-03367 and K6 CA 14, 219. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 225-229 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Spleen colonies produced by sublethally irradiated mouse bone marrow cells were compared to those produced by unirradiated marrow cells in lethally irradiated mice. Sublethally irradiated marrow cells gave rise to many fewer spleen colonies. At seven days of colony age, the ratio of erythroid colonies to granuloid colonies was lower (〈 1) than for colonies formed by unirradiated marrow (2 to 3 or more). Delay of harvest of colonies to day 10 or 12 resulted in 6 to 11 fold increase in the ratio of erythroid to granuloid colonies due largely to the belated appearance of erythroid colonies.
    Additional Material: 6 Tab.
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    URL: http://dx.doi.org/10.1002/jcp.1040750211
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  • 108
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    Membranes of animal cells. V. Biosynthesis of the surface membrane during the cell cycle (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 275-279 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: KB cells grown in suspension culture were synchronized by using a double thymidine block. At various times throughout the life cycle aliquots of cells were pulsed with 14C-L-leucine, 14C-D-glucosamine and 14C-choline for one hour periods. Surface membranes, cell particulates and soluble proteins were isolated and their 14C specific activities were determined. It was found that there was a marked increase in the rate of incorporation into surface membrane just after division. The pattern of incorporation was the same for all three isotopic precursors. The rate of incorporation of isotopic precursors into soluble proteins was constant throughout the cycle. Some increase in rate of incorporation of isotope into the particulate fraction was observed during division.
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    URL: http://dx.doi.org/10.1002/jcp.1040750303
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  • 109
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    Synthesis by a clonal line of rat glial cells of a protein unique to the nervous system (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 329-339 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The synthesis of a protein unique to the nervous system, the “S100-protein,” has been studied in a clonal line of rat glial cells. It has been shown that these cells do not begin to accumulate “S100-protein” until the cultures enter a phase of density-dependent inhibition of cell proliferation. Further experiments indicate that the regulation of “S100-protein” accumulation resides at least in part in an interaction involving the cell surface.
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    URL: http://dx.doi.org/10.1002/jcp.1040750309
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  • 110
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    Thymidine as synchronizing agent. III. Persistence of cell cycle patterns of phosphatase activities and elevation of nuclease activity during inhibition of dna synthesis (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 297-303 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Synchronous cultures of HeLa cells were obtained by selective detachment of cells in mitosis and fluctuations in enzyme activity were followed during the subsequent cell cycle. The enzymes measured were alkaline and acid phosphatases and a nuclease active on denatured DNA at alkaline pH (alkaline DNase). Each of these enzymes showed a different pattern of activity in the cell cycle, but a temporal relationship to the DNA synthetic phase was apparent in each case. Treatment of the cultures at the beginning of the cell cycle with 15 mM thymidine did not alter the subsequent pattern of fluctuations in activity of alkaline phosphatase or of acid phosphatase, although DNA synthesis was fully inhibited by this treatment. This indicates that the pattern of activity of some enzymes is not linked to DNA replication. On the other hand, the pattern of fluctuations in the activity of alkaline DNase was abolished by thymidine treatment, and elevation of the activity of this enzyme was observed. These results suggest complex and variable relationships between phases of the cell cycle and enzyme activity, and show that inhibition of DNA synthesis is not a suitable procedure for induction of culture synchrony if enzyme activities are to be studied.
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    URL: http://dx.doi.org/10.1002/jcp.1040750306
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  • 111
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    Beneficial effects of embryo extract on cultured rabbit cartilage cellsThis research has been supported by grant GB 7007 from the National Science Foundation and grant E-505 from the American Cancer Society. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 353-360 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Growth and the expression of cartilage-like differentiation by adult rabbit chondrocytes is stimulated greatly by the Sephadex G-25 macromolecular fraction of chicken embryo extract (CEEM). A small amount of fetal bovine serum or the macromolecular fraction of fetal bovine serum (FBSM) is also needed for growth. Medium F12 supplemented with CEEM plus FBSM supports moderate clonal growth of the rabbit chondrocytes with excellent expression of differentiated properties. The effects of CEEM on growth and the expression of differentiation have been quantitated by measurement of radioactive sulfate incorporation and total protein synthesis.
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    URL: http://dx.doi.org/10.1002/jcp.1040750311
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  • 112
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    In vitro immune response of primed mouse spleen cells as affected by the time of antigenic stimulationSupported by CNEN-Euratom Association Contract. Publication 571 of the Euratom Biology Division. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 3-6 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Normal and primed spleen cells stimulated in vitro with sheep RBC showed a different behaviour in the 19 S PFC response. The immunologic capacity of primed cells, unlike that of unprimed cells, was impaired if the antigen addition to culture had been delayed for more than six hours. The requirement of primed cells for immediate reexposure to antigen varied with the time of preimmunization and was maximum when spleen cells had acquired in the donor the ability to display the highest response in vitro upon optimal restimulation. This phenomenon of antigen requirement of primed cells in vitro is at variance with the known immunologic behaviour of primed cells grown in vivo, and can be attributed to cellular changes unmasked by in vitro cultivation.
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    URL: http://dx.doi.org/10.1002/jcp.1040760104
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  • 113
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    Histidase function in human epidermal cells (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 7-15 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The activity of histidase was studied in (1) epidermal tissue scraped from human infant foreskin, (2) fibroblast-like cells in monolayer serial culture from human foreskin, and (3) epithelial-like (epidermal) outgrowth from foreskin primary explants. Foreskin epidermal tissue without in vitro culture and epidermal outgrowth in primary culture from explants of foreskin showed equivalent mean levels of histidase activity, 5.22 × 10-3 and 5.01 × 10-3 μMoles urocanic acid produced per milligram protein per minute. Under the same assay conditions, there was no measurable histidase activity in cultured fibroblast-like cells from foreskin at various times after subculture. The Km for enzyme from human foreskin epidermal tissue ranged between 2 and 5 × 10-3 M histidine. Ability to demonstrate the presence or absence of this tissue-specific enzyme function in cultured cells suggests a useful means for studying differentiation, as well as a more precise way to identify epidermal origin of cultured cell types than morphological characteristics alone would permit.
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    URL: http://dx.doi.org/10.1002/jcp.1040760105
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  • 114
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    A new method of cloning mammalian tissue culture cells in suspensionThis work was performed under the auspices of the U. S. Atomic Energy Commission. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 85-88 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Chinese hamster cells (line CHO) were cloned in suspension with a carboxymethylcellulose-containing medium, and the colonies were counted with an electronic particle counter after enzymatically degrading the carboxymethylcellulose with cellulase. Survival of x-irradiated cells was determined by both the standard plating technique and the suspension technique, and comparable results were obtained.
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    URL: http://dx.doi.org/10.1002/jcp.1040760112
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  • 115
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    Masthead (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970) 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040760201
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  • 116
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    Density distribution analysis of In vivo and In vitro colony forming cells in developing fetal liverPublication 1323 from The Walter and Eliza Hall Institute.This work was supported by grants from The National Health and Medical Research Council, Canberra, and the Australian Research Grants Committee. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 181-192 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The technique of buoyant density separation in gradients of Bovine Serum Albumin has been used to separate in vivo and in vitro colony forming cells (C.F.C.'s) in hemopoietic tissue of mouse fetal liver. Differences in the density distribution profiles showed that the in vivo and in vitro C.F.C.'s were different cell populations but the existence of an “out-of-phase” density association suggested that the two cell types were closely related.Complex density heterogeneity of both cell populations was observed at later stages of liver development and was similar to that seen in adult marrow. A homogeneous population of in vivo and in vitro C.F.C.'s occupied a very light density position in 10.5 day fetal liver. The subsequent development of density heterogeneity was associated with progressive acquisition of higher density subpopulations. Transfer experiments showed the capacity of the lightest density cells from the earliest stage of liver hemopoiesis, to generate higher density colony forming cells in the environment of the adult marrow. Density determined differences in seeding efficiency of in vivo C.F.C.'s were observed but no evidence was obtained for differences in either in vivo or in vitro colony morphology in different density subpopulations.
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    URL: http://dx.doi.org/10.1002/jcp.1040750207
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  • 117
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    Calcium transport and distribution in ehrlich mouse ascites tumor cells (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 231-240 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Data from isotopic uptake experiments were used to measure calcium fluxes and compartment sizes in ascites tumor cells. The data were analyzed with two kinetic models, A and B. In 80% of the experiments model A, which is based on one exchangeable calcium compartment, was rejected in favor of Model B, which predicts two exchangeable compartments. A statistical evaluation of the model's performance, when fit to the experimental data was used to select between the two models.The results show that calcium was distributed between three cellular compartments in the ratio, non-exchangeable (88%): rapidly exchanging (7%): slowly exchanging (5%). The undirectional fluxes suggested that calcium transport could be described as a series system with the temporal sequence: environment ⇄ rapidly exchanging ⇄ slowly exchanging.
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    URL: http://dx.doi.org/10.1002/jcp.1040750212
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    The interaction of mengo virus ribonucleic acid with L, HeLa and human diploid cells: A comparative studyThese investigations were supported by a research grant from the National Cancer Institute of Canada. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 253-262 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A study, designed to define the optimal conditions for infecting L, HeLa and human diploid cells with Mengo RNA, showed that the three cell types differ with respect to (a) the concentration of sucrose (in PBS) that produces maximum stimulation of infectious center (I.C.) formation (0.6 M, 1.2 M and 1.0 M for L, HeLa and human diploid cells respectively) and (b) the optimal combination of sucrose and dimethyl-sulfoxide (DMSO). With all three cell types, diethylaminoethyldextran (DEAE-D) was found to produce maximum stimulation of I.C. formation when present, in PBS, at a concentration of 100 μg/ml. Direct comparisons of I.C. formation in the three cell types using the sucrose, sucrose-DMSO and PBS-DEAE-D media optimal for each revealed that (a) the best medium for titration in L and HeLa cells is PBS containing 100 μg DEAE-D/ml, (b) DMSO produces a 4-8 fold increase in I. C. formation in L cells, but is only marginally effective in HeLa cells, and (c) the optimal medium for titration in human diploid cells is sucrose-DMSO. The results of studies of the “uptake” of 3H-Mengo RNA showed that there is no correlation between the amount of RNA that becomes cell associated and the number of I.C.'s that are produced in the cell population.
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    URL: http://dx.doi.org/10.1002/jcp.1040750214
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    Comparative aspects of poliovirus replicationSupported by USPHS Research grants AI 06862 and AI 08864 and Training grant AI 00299. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 265-271 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: To the extent that parallel experiments with picornaviruses and RNA bacteriophages can be compared, the results suggest that their mechanisms of RNA replication are basically similar, despite fundamental differences between the two groups of viruses with respect to the control of the expression of genetic information.The significance of the extracted multi-stranded replicative forms of viral RNA is discussed and it is concluded that, if not identical to the corresponding, intracellular forms, the extracted structures are meaningful derivatives of specific native forms.
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    URL: http://dx.doi.org/10.1002/jcp.1040760306
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    The action of mendelian genes in human diploid cell strainsThe original investigations reported in this paper were supported by NIH Research grant 1-P01-15419-03. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 311-330 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Some of the cells of every human being will grow outside the body as microorganisms. It is possible to show, in a variety of ways, that these cells resemble genetically the individual from whom they were obtained. Over 35 inherited human diseases and anomalies can now be studied in such cell lines.Human diploid cell strains, biochemically marked by one or more mutant Mendelian genes, have proven particularly useful for the study of gene action in man and for the detection of genetic changes such as mutation and somatic cell hybridization. In addition, the strains have a number of clinical applications, including the antenatal diagnosis of inherited disease.The failure of cultured human cells to display their phenotype at most loci continues to restrict their use in both genetics and medicine. There are reasons for hoping that this difficulty will eventually be solved, and some experiments bearing on the problem are already feasible.
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    URL: http://dx.doi.org/10.1002/jcp.1040760310
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    Flattening, movement and control of division of epithelial-like cellsThis work was begun at the Biochemical Research Foundation, Newark, Delaware prior to its merger with The Institute for Cancer Research. Supported by U.S.P.H.S. Research Grants CA-07846, CA-10367, CA-06927 and FR-05539 from the National Cancer Institute, and by an appropriation from the Commonwealth of Pennsylvania. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 57-64 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The kinetics of cell division and movement in four epithelial-like cell lines, grown in continuously perfused culture medium, were studied by time-lapse cinemicrography. One line exhibited “contact regulation of cell division,” so that the rate of mitosis per cell decreased steadily as population density increased. In the other three lines mitosis was not controlled as a function of population density until the cells became very crowded. An explanation for this difference was sought in terms of the hypothesis that the rate of division depends on the area of the cell membrane.Cells of the contact-regulated line flattened uniformly on the substrate. Their motility was restrained by adhesion between their borders. As they crowded together, contact inhibition of cell overlap caused a steady decrease in average surface area per cell. All three of the non-controlled lines also had contact inhibition of overlap. Cells of two of them flattened on the substrate; but these cells had little mutual adhesion and were highly motile, so that they continually changed their shapes. The areas of their cell membranes were therefore not subject to a restraint that could control the rate of division. Cells of the fourth line remained rounded or only slightly flattened during culture growth, so that no change in cell membrane area occurred that could change the rate of division.
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    Characterization of RNA molecules restricted to the nucleus in mouse L-cellsThis investigation was supported by U. S. Public Health Service Training grant 00182 and Research grant GM 12449 from the Institute of General Medical Sciences. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 97-106 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: RNA molecules which are restricted to the nucleus in mouse L-cells were characterized by the technique of RNA/DNA hybridization. Competition of cytoplasmic RNA with labeled nuclear RNA of various sizes revealed that the RNA restricted to the cell nucleus is heterogeneous in size. Competition for sites on fractions of mouse DNA of various base compositions indicated that this unstable RNA is also heterogeneous in base composition. Fractionation of nuclei into three subfractions failed to separate the uniquely nuclear RNA from the precursors of cytoplasmic RNA. The significance of the selective transport of RNA from the nucleus to the cytoplasm and its importance in the control of gene activity in eucaryotic cells is discussed.
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    A melanin-dispersing effect of cyclic adenosine monophosphate on Fundulus melanophoresSupported by NSF grant GB-4956X. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 133-135 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Treatment of Fundulus melanophores with adenosine 3′,5′-monophosphate (cyclic AMP) is followed by reversible melanin dispersion in these cells. Adenosine 3′-monophosphate and adenosine 5′-monophosphate both have a similar, but weaker dispersing action. In addition, adenosine 5′-monophosphate also has a melanin aggregating effect. These results are interpreted to mean that nerve transmitters may act by controlling the level of cyclic AMP within the Fundulus melanophore.
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    Masthead (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970) 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040750201
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    DNA-mediated transformation of mammalian cells in culture. Increased transforming efficiency following sonicationThis work was supported, in part, by USPHS Research grants RO1-CA04534 from the National Cancer Institute and RO1-GM14514 from the National Institute of General Medical Sciences. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 137-150 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A series of 100 experiments was completed to determine if DNA is capable of transforming the genotype of a murine lymphoma (P388) in cell culture. The test system was concerned with the transformation of cells from 8-azaguanine (AZG) sensitivity to resistance. By the use of this marker, it was determined that transformation by DNA did occur, and that the efficiency of transformation was greatly increased by sonication of the DNA. A statistical analysis of 100 experiments demonstrated that the increase in the number of resistant cells after treatment with sonicated resistant DNA (R-DNA) was statistically significant (χ2 〉 4.25, 0.05 〉 p 〉 0.02) in 66% of the experiments. DNA from sensitive parental cells and DNA from other sources produced no effect while DNase and UV treatment abrogated effective transformation by either sonicated or nonsonicated R-DNA. RNase was without effect.Sucrose gradient analysis of sonicated and nonsonicated R-DNA demonstrated that the peaks which correspond to the highest specific transforming activity are not altered by sonication and do not coincide with the OD260 peaks, in spite of the fact that sonication shifted the peak of maximum OD260 to a slower sedimenting region of the gradient. The major portion, however, of the transforming material did shift after sonication to the slower sedimenting region of the gradient and did coincide with the OD260 peak.The hereditary stability of the transformed cells was established by cloning a representative number of transformants, growing them in the absence of AZG for an extended period and then testing their ability to grow in graded concentrations of AZG. In addition, DNA extracted from transformants successfully transformed sensitive cells.
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    Interaction of calcium and local anesthetics with skeletal muscle microsomesThis work was submitted (by A.B.) in partial fulfillment of the Ph.D. degree in pharmacology, University of Utah College of Medicine. It was supported by USPHS research grant 5PO1 NB04533 and by USPHS research career development award 5KO3 NB08641 (R.K.). (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 199-211 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The influence of Ca++, several drugs, and pH on the binding of Ca++ by skeletal muscle microsomes was studied in vitro. A mass-law graphic analysis revealed the presence of three distinct species of Ca++-binding sites in the microsomes, and the binding at only one of these sites was antagonized by local anesthetics and quinidine. These drugs also decreased the maximum Ca++-binding capacity of the microsomes. Caffeine and ouabain exerted no effect on the binding at any of the sites. Procaine was also bound by microsomes, and this binding was antagonized by Ca++, which also decreased the maximum procaine-binding capacity of microsomes. The sites that bind procaine and Ca++ are not identical because the maximum-binding capacities of the interacting sites are distinctly different. The influence of pH on the ability of drugs to antagonize Ca++ binding indicates that the displacing activity increases as the percentage of the drug in the nonionized form increases. All of the data obtained in the above studies are consistent with the interpretation that quinidine and local anesthetics of the procaine type noncompetitively antagonize the binding of Ca++ by microsomes. The pharmacological significance of a noncompetitive interaction may be related to the property of local anesthetics and quinidine to increase contractile tension in skeletal muscle rather than to their ability to stabilize the cell membrane.
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    Errata (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 263-263 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040750215
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    Stimulation of DNA synthesis in cultures of mouse embryo fibroblast-like cells (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 305-313 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Stimulation of the DNA synthesis and mitoses in stationary cultures of mouse embryo fibroblast-like cells was induced by various agents such as ribonuclease, digitonin, fresh medium and commercial preparations of hyaluronidases. Time sequence of stimulation was similar in experiments with all these agents. Cells were activated to enter S phase from GI phase. The rise of the number of DNA-synthesizing cells was preceded by a latent period of about 8-12 hours with the maximal number of DNA-synthesizing cells being observed at 16-24 hours. Mitotic wave was observed after the wave of DNA synthesis. Stimulation of DNA synthesis and mitosis was not preceded by any significant decrease of an average cell density in the culture. The progeny of activated cells had no greater chance than other cells to be activated again when stimulation was repeated.It is concluded that similar proliferative reactions can be induced in stationary cultures by a variety of diverse agents. Possible role of cell surface changes in the induction of these reactions is discussed.
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    Masthead (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970) 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040760101
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    Control of multiplication of uninfected rat cells and rat cells converted by murine sarcoma virusThis paper is number XIII in a series “Carcinogenesis by RNA sarcoma viruses.” The previous paper in this series is Altaner and Temin ('70). The previous paper on control of cell multiplication is Temin (1969a). The previous paper on multiplication of rat cells is Temin ('68b).This investigation was supported by Public Health Service Research grant CA 07175 from the National Cancer Institute. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 107-119 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The rate of multiplication of rat embryo fibroblasts in monolayer culture depends upon the amount of multiplication-stimulating activity in the culture medium, as well as the efficiency of stimulation by and utilization of this activity. Multiplication-stimulating activity is defined by its capacity to stimulate DNA synthesis and cell division in stationary populations of cells. Usually, multiplication stimulating activity is supplied as serum in cell culture media, but rat cells also produce it.A comparison of multiplication of uninfected and Murine Sarcoma virus-converted rat cells showed that converted cells multiplied at a greater rate than did uninfected cells, with the use of less or the same amount of multiplication-stimulating activity; the converted cells produced cells produced an inhibitor of multiplication-stimulating activity, and the efficiency of stimulation of DNA synthesis was similar for uninfected and converted cells. It appears that in the presence of serum the efficiency of utilization of multiplication-stimulating activity is greater for converted cells than for uninfected rat cells.
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    The use of pronase in tissue culture: A comparison with trypsin (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 159-161 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Pronase is a proteolytic enzyme recently introduced as an effective dispersing agent of tissue culture cells. We have found it to be a most rapid and complete dispersing agent for primary fibroblastic cell lines and clearly superior to trypsin. On the other hand, with certain continuous epithelial cell lines its completeness of dispersion is inferior to that of trypsin necessitating study of each cell line individually with both enzymes to select the most advantageous one.
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    Strain-specific mitotic inhibition in large mononucleate amoebae (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 193-197 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A strain-specific antimitotic substance is present in large mononucleate amoebae. It inhibits mitosis when injected into amoebae of heterologous strains. The antimitotic substance has been isolated from homogenized cells, and some of its properties have been studied. The results obtained show that the active factor is a protein(s) of molecular weight between 200,000 and one million. The activity of this substance differs from other known antimitotic agents in that its action is strain-specific.
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    The possible mediation by cyclic AMP of parathyroid hormone-induced stimulation of mitotic activity and deoxyribonucleic acid synthesis in rat thymic lymphocytesIssued as NRCC no. 11237. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 213-224 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Purified parathyroid hormone (PTH) strongly stimulates the initiation of deoxyribonucleic acid (DNA) synthesis and thereby raises the flow of cells into mitosis in rat thymic lymphocyte populations maintained in vitro. These actions of PTH are potentiated by caffeine and inhibited by imidazole which indicates that the hormonal action is mediated by cyclic adenosine 3′,5′,-monophosphate (cyclic AMP). The feasibility of cyclic AMP being the mediator of PTH action is established by the observation that a low concentration (10-7 M) of dibutyryl cyclic AMP precisely mimics the stimulatory action of the hormone on DNA synthesis and cell proliferation.
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    The possible mediation by cyclic AMP of the stimulation of thymocyte proliferation by vasopressin and the inhibition of this mitogenic action by thyrocalcitoninIssued as NRCC 11451. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 65-76 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Lysine vasopressin (antidiuretic hormone), like cyclic adenosine 3',5'-monophosphate (cyclic AMP), rapidly (in less than 1 hour) stimulates the initiation of deoxyribonucleic acid synthesis and thereby increases the flow of cells into mitosis in rat thymic lymphocyte populations in vitro. This mitogenic action of vasopressin, again like that of cyclic AMP, is potentiated by caffeine, an inhibitor of the intracellular phosphodiesterase which catalyzes the degradation of cyclic AMP. On the other hand, vasopressin's mitogenic action (also like that of cyclic AMP) is blocked by imidazole, an activator of cyclic nucleotide phosphodiesterase activity. The hormone, thyrocalcitonin (calcitonin) which is known to block the cyclic AMP-mediated mitogenic effect of parathyroid hormone by interfering with cyclic AMP action, also blocks the mitogenic action of vasopressin. The inhibitory effects of imidazole and thyrocalcitonin on vasopressin's mitogenic action are both overcome by the phosphodiesterase inhibitor, caffeine. It is concluded from these observations that the mitogenic action of vasopressin is mediated by cyclic AMP.
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    Lipid composition of heteroploid cells in culture: Effects of prednisoloneSupported, in part, by a Public Health Service research grant (CA-08315) from the National Cancer Institute. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 101-106 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Prednisolone-induced alterations in the content and composition of the total lipids, cholesterol, phospholipids and fatty acids were studied in HeLa S3 cells. After relatively long exposure of the cell cultures to the hormone analog (24 to 72 hours), total cell lipid content was decreased. Partial inhibition of cholesterol and phospholipid metabolism resulted in a shift in the molar ratio of these two lipid constituents. Total fatty acid content was unaffected by prednisolone but there were minor changes in the relative distribution of the individual fatty acids.The observed decrease in cholesterol turnover after addition of prednisolone was primarily due to the reduced uptake of intact cholesterol from the culture medium. This was compensated, in part, by an increased de novo synthesis of cholesterol from acetate and glucose.
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    Parathyroidectomy and the induction of thymic atrophy in normal, adrenalectomized and orchidectomized ratsIssued as NRCC no. 11591. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 141-149 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Parathyroidectomy or thyroparathyroidectomy in the male rat results in the severe atrophy of the thymus gland. This effect is not mediated by a secondary release of the cytolytic adrenal or gonadal steroids since it also occurs in adrenalectomized and orchidectomized animals. The loss of the parathyroid gland decreases the flow of thymic lymphocytes into mitosis; this inhibition of cell production leads to the marked decline in thymic weight. The effect can be partially prevented by administration of parathyroid hormone or calcium gluconate.
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    The influence of diffusion in the external medium upon the determination of cytoplasmic diffusion coefficients (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 167-173 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The exchange of isotopic water has been studied in ovarian eggs from three Anuran species (R. esculenta, R. pipiens and R. temporaria) by the automatic diver balance technique. The oocytes were treated with chemicals (digitonin, ethanol and formaldehyde) to remove the plasma membrane. The cytoplasmic diffusion coefficient for water was calculated with and without allowance for the influence of diffusion in the external medium. The former expedient was found necessary in order to obtain correct values. The chemical treatments gave different values, but there are sound reasons to believe that those obtained with digitonin (8.9, 15.3 and 10.8.10-6 cm2sec-1 for R. esculenta, R. pipiens and R. temporaria, respectively) are the correct ones. These results indicate that the rate of diffusion in the oocyte cytoplasm amounts to 50-75% of that of free diffusion.
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    Hemopoietic environment necessary for an early stage in differentiation of antibody-forming cells: Effect of cortisolSupported in part by grant T-476 and Institutional Research grant IN-54 from the American Cancer Society, and grant GR68RP1 from the United Health Foundation of Western New York. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 197-205 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Treatment of adult mice with cortisol decreased the number and frequency of bone marrow precursors of anti-sheep erythrocyte hemolytic plaque-forming cells (P-PFC) and marrow “lymphocytes.” However, the more primitive progenitors of P-PFC in marrow were not affected. Treatment of prospective recipients of marrow grafts with cortisol impaired the generation of P-PFC by transplanted primitive progenitor cells. Differentiation with proliferation of P-PFC into mature PFC was less dramatically affected by cortisol. P-PFC were not apparently suppressed directly, since the fall-off of P-PFC did not occur during the first day after administration of cortisol. Thus, cortisol impairs the generation of P-PFC by altering a hemopoietic inductive environment necessary for this step in differentiation of immunocompetent cells.
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    The effect of drugs on the rate of proteolysis in Vitro within secondary liver lysosomes (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 225-229 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The in vitro hydrolysis rate of intravenously injected 131 I-albumin inside mouse liver phagolysosomes was studied for this type of secondary lysosome, when incubated at pH 5.0 and 37°C in the presence of different drugs. While vitamin A alcohol and etiocholanone showed inhibitory effects, suitable concentrations of chloroquine activated that intraparticulate hydrolysis. On the contrary cortisone, in various concentrations, did not alter the hydrolysis rate of the labeled albumin. Neither cortisone nor chloroquine modified the inhibitory effect of vitamin A. The hypothesis that the drugs act on the hydrolysis rate because they modify the stability of this type of secondary lysosome is forwarded.
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    Grand slam or of the life and achievements of harry eagle, scientist, Recorded 65 years after his birth by his friend and admirer (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 237-239 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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    URL: http://dx.doi.org/10.1002/jcp.1040760303
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    Variations in the synthesis and assembly of immunoglobulins by mouse myeloma cells: A genetic and biochemical analysis (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 331-348 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The synthesis, assembly, and secretion of gamma globulin has been examined using cells from a mouse plasma cell tumor. The cells were then adapted to continuous culture and individual clones were obtained which differed from each other in the relative amounts of heavy and light chains synthesized, in the absolute amounts of gamma globulin being produced, and in the details of the assembly process. Finally, the kinetics, pathway, and completeness of assembly were examined in a variety of tumors and variants of these tumors.
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    Inhibition of RNA synthesis by actinomycin D: Characteristic dose-response of different RNA species (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 127-139 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A quantitative dose-response study was made of the inhibition by actinomycin D of the synthesis of various RNA species in cultured L cells. After a suitable preincubation with various concentrations of actinomycin, synthesis was measured by a brief incorporation of radioactive uridine. The 45S precursor of ribosomal RNA, 5S ribosomal RNA, transfer RNA and various sized fractions of heterogeneous nuclear RNA were extracted from the appropriate subcellular fractions and resolved by acrylamide gel electrophoresis. Polyribosomal messenger RNA was also studied. Over the range of actinomycin concentrations employed cellular uptake of the drug is proportional to external concentration, and maximal inhibition of RNA synthesis is generally achieved within one hour after exposure.For most of the RNA species studied the dose-response data appear to follow an exponential inactivation function with some “tailing” evident at the higher doses. Sensitivities, defined as the reciprocal of the dose necessary to reduce synthesis to 1/e of the control level, were compared and examined with respect to the molecular weight of the RNA species under consideration. Among the heterogeneous nuclear RNA's there is a good correlation between RNA size and sensitivity, such that the sensitivity per unit molecular weight is relatively constant. A similar relationship is roughly applicable to the ribosomal and transfer RNA's when taken as a group, although in this case the sensitivity per unit molecular weight is 50 to 100 fold greater.A model is proposed in which this marked difference in actinomycin sensitivity arises as a consequence of the transcription of stretches of contiguous repetitive genes by RNA polymerase molecules which attach to the DNA only at a limited number of entry points, the highest probability of attachment being at the beginning of a stretch.
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    Further studies on the biological properties of friend virus-induced leukemic cells differentiating along the erythrocytic pathwayResearch supported by grants CA-10,000 from NCI-NIH, USPHS, U-1840 from the New York Health Research Council, and the Leukemia Society of America, Inc. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 159-166 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: When Friend virus-induced leukemic cell lines were injected into irradiated hosts after the second radiation dose, the colony-forming unit (CFU) in the recipient spleens per 104 cells was found to be 7-fold higher than the CFU obtained when the second radiation dose had been given shortly after the inoculation of the cells. Serial passage of the cells from the spleen colonies to irradiated hosts resulted in a marked increase of the CFU value, indicating that this cell population was capable of both self-replication and erythroid differentiation. The “f” fraction, which indicates the percentage of the inoculated cells that reach the spleen in the irradiated recipients, was found to be approximately 15%. If the highest CFU value obtained from serial colony-to-colony passages is corrected by this factor, a final cloning efficiency of about 18% is demonstrated.Neither induced plethora nor the administration of erythropoietin (1 u/mouse/for 2 days) appeared to affect the spleen colony-forming ability of the leukemic cells.Erythroid differentiation is not detectable in the transplantable subcutaneous tumors which were used to initiate the tissue culture lines and which also are capable of inducing erythroid spleen clones in irradiated recipients. This lends support to the theory of the influence of “microenvironmental factors” on the fate of stem cells with potential for differentiation.
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    Argon laser micro-irradiation of mitochondria in rat myocardial cells in tissue cultureThis research was supported by the US Army Medical and Development Command, Office of the Surgeon General, under Contract DA-49-193-MD-2564. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 207-213 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Three types of lesions produced by argon laser micro-irradiation of single mitochondria are described. A correlation between lesion severity and optical phase density and/or laser output power was observed. Cell survival was generally not affected by mitochondrial irradiation. As many as ten mitochondria were irradiated without cell death resulting. Absorption of the laser radiation was attributed to the natural chromophores, cytochromes c and c1.
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    Selection of synchronous Chlorella cells by centrifugation to equilibrium in aqueous ficollThis research was supported in part by National Science Foundation grant GB-17305 and National Aeronautics and Space Administration grant NGR 47-004-006. Dr. H. A. Hopkins was supported as a research associate by Public Health Service grant 5-SO5-FR07095-02. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 231-233 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Synchronous daughter cells of Chlorella pyrenoidosa can be selected, on the basis of their unique density, from cultures growing in continuous light by centrifugation to equilibrium in aqueous Ficoll. The degree of synchrony, as judged from cell release data, is equivalent to or better than that obtained by intermittent-illumination procedures.
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    Defective bacteriophages (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 253-263 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Naturally occurring defective phage particles, which do not form plaques on any known host, but have a restricted host killing range, appear to be widely distributed. The defective phages are produced spontaneously but can be induced, at much higher levels, by chemical and physical agents which interfere with metabolism or structure of DNA. The defective phages discussed in this article have been divided into various categories on the basis of their structural complexity, which ranges from what appears to be phage tail components through to intact phage particles, and the source of the DNA packaged into the heads of the phage-like particles. The evolution of the defective phages is discussed and the possibility is entertained that they may have originated from temperate phages.
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    From cell biology to immunology - A short tripSupported by N.I.H. grants CA 04681, AI 08917 and 2 ROI HD 01287. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 303-310 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Immunologic memory and immunoglobulin allotype suppression are discussed as problems in Cell Biology. Memory, the ability of an animal after a first antigenic exposure to give a heightened and faster immune response upon a second exposure to the same antigen, is shown to be a property of bone marrow-derived cell lines. Expression of this memory depends on interaction with thymus-derived cells from either non-immunized or immunized mice.Chronic allotype suppression is described for the first time. It is initiated by in utero or neonatal exposure of (SJL × BALB/c)F1 mice, allotype a/b, to anti b antibody. Suppression lasts for long periods and continues in irradiated recipients which have received lymphoid cells from suppressed donors. Selection against b allotype producing cell precursors can explain suppression.
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    Responsiveness of normal and transformed rat embryo cell cultures to poly I · poly C and interferon (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 365-371 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A number of normal rat cell cultures as well as cultures transformed spontaneously, by chemicals, and/or by oncogenic viruses were tested for responsiveness to the interferon inducer polyinosinic·polycytidylic acid, or to exogenous interferon. Responsiveness, or lack thereof, had no correlation with subculture passage number, infection with RNA leukemia virus, morphological transformation by oncogenic RNA or DNA viruses, chemical treatment, or the ability of these cells to produce tumors in isologous host animals. The data indicate that lack of response to interferon or to the inducer is neither a necessary prerequisite nor an absolute result of cellular transformation of rat cells.
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    The dependence of pacemaker discharge of Aplysia neurons upon Na+ and Ca++ (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 121-127 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In total absence of Na+ some identified neurons of Aplysia, after a period of silence, resume pacemaker discharge in the normal pattern with normal action potentials, while other identified neurons remain silent. In absence of Ca++ all pacemaker neurons increase spontaneous discharge and develop abnormal bursting patterns. Those neurons which discharge spontaneously in Na+ free solutions show much less dependence on Na+ and much greater dependence on Ca++ for action potentials initiated by electrical stimulation than do those neurons which do not fire spontaneously in absence of Na+. In absence of both Na+ and Ca++ all neurons become inexcitable, but much more rapidly at higher temperatures.
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    Evidence for the existence of multipotential lympho-hematopoietic stem cells in the adult ratThis investigation was supported in part by Research grants CA-10320 and AI-07001, training grant HD-00030, and career development award CA-09873 from the Public Health Service; and by an American Cancer Society Institutional grant IN-38-J. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 151-158 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Rats were given near-lethal doses of x-ray to produce clones of hemic cells marked by radiation-induced chromosome abnormalities. Subsequently, bone marrow from these rats was injected into lethally irradiated mice to form erythropoietic spleen colonies; and peripheral blood lymphocytes from the same rats were stimulated to proliferate in a mixed lymphocyte interaction (MLI), an immunological response to histocompatibility isoantigens.Chromosome markers indicated that in several instances the cells of an erythroid spleen colony and a proportion of the lymphocytes reacting in the MLI were progeny of the same stem cell in the donor rat. In addition, lymphocytes of the same radiation-marked clone were shown to proliferate in response to several different histocompatibility isoantigens. The data indicate the presence in the adult rat of a primitive lymphohematopoietic stem cell capable of yielding both erythroid and lymphoid progeny. The findings also suggest that immunological specificity is determined during lymphoid differentiation, subsequent to the stem cell stage.
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    The diversity of sulfhydryl groups in the human erythrocyte membraneThis investigation was supported by Public Health Service Research grant HE-08221-06, National Institutes of Health. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 281-291 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Human bank blood erythrocytes were exposed to the mercurials p-chloromercuribenzoate (PCMB), chlormerodrin (CM), p-chloromercuribenzenesulfonate (PCMBS), and 1-bromomercuri-2-hydroxypropane (BMHP) for different time intervals, at different concentrations and in combination with n-ethylmaleimide (NEM) added before, and 2-mercaptoethylguanidine (MEG) and reduced glutathione (GSH) added after the mercurial. Binding patterns of the mercurials to the cells and effects on permeability of the cells were measured. The results indicate that the erythrocyte membrane contains multiple classes of sulfhydryl groups, alteration of which has a variety of effects on cell permeability. PCMB, chlormerodrin and PCMBS react with at least three classes of sulfhydryls, two of which are associated with the sodium-potassium barrier and, when altered, result in potassium loss, sodium accumulation and hemolysis. BMHP reacts with at least two classes of sulfhydryls, one of which is associated with permeability, and, when altered, results in hemolysis in isotonic solutions of choline chloride or lactose. The results provide additional insight into the structure and function of the erythrocyte membrane.
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    Announcement of editorial change (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. i 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040760102
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    Human bone marrow colony growth in agar-gelSupported by grants from the American Cancer society (E-498) and the National Cancer Institute, National Institutes of Health (I ROI CA 11305-DI). (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 77-84 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A technique for growing human bone marrow cell colonies in agar-gel medium is described. “Feeder layers” containing 1 × 106 normal human peripheral white blood cells are used as the stimulus for colony growth. Human bone marrow aspirates are collected in heparinized syringes and plated as 2 × 105 cells on “feeder layers.” Normal human bone marrow yields 32-102 colonies per 2 × 105 cells plated. Colonies are almost exclusively granulocytic. Growth rate of colonies is slower than with mouse bone marrow but colonies reach a comparable size (500-1500 cells) at days 12-16.
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    Mammalian cell fusion III. A hela cell inducer of premature chromosome condensation active in cells from a variety of animal species (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 151-157 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The phenomenon of premature chromosome condensation (PCC) is induced in unstimulated horse lymphocytes, bovine spermatozoa, Chinese hamster ovary cells, embryonic chick fibroblasts and erythrocytes, Xenopus kidney and mosquito cells by fusing each of these cell types with HeLa cells blocked in mitosis. Thus it becomes possible to visualize chromosomes even from non-multiplying cells of heterologous species, such as, chick erythrocytes and bovine spermatozoa.
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    Extraction of an actomyosin-like protein from amoebae of Dictyostelium discoideum (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 185-190 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An actomyosin-like protein has been extracted from amoebae of Dictyostelium discoideum, V-12. The purified protein exhibited a reversible change in viscosity upon addition of ATP, indicating an ATP sensitivity of 75-85% and a specific viscosity of 0.1. At low ionic strength in the presence of Mg++ and ATP the amoeba protein displayed the phenomenon of superprecipitation. The protein extract was found to be an adenosinetriphosphatase (ATP'ase) hydrolyzing ATP to ADP and inorganic phosphate. Both Mg++ and Ca++ at low ionic strength accelerated the ATP ase activity whereas at high ionic strength only Ca++ stimulated ATP hydrolysis. The ATP'ase activity was inhibited by ethylene-diamine-tetraacetic-acid, Mersayl and p-chloromercuribenzoate. The physico-chemical and enzymatic properties of the extracted amoeba protein are qualitatively comparable to those of muscle actomyosin, and very similar in quantitative properties to smooth muscle actomyosin and the actomyosin-like proteins of blood platelets, leucocytes and slime mold plasmodia. The significance of the presence of this actomyosin-like protein in Dictyostelium amoebae is discussed in relation to amoeboid form and movement.
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    An apparent discrepancy between the number of colony forming units transplanted and survival of busulphan lethally treated rats (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 215-223 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Conditions for keeping busulphan lethally treated rats alive by transplantation of bone marrow cells from syngeneic donors are described. After busulphan treatment of the donor rats with a dose which only reduces the colony forming units (CFU's) in the marrow (assayed by the spleen colony technique) to half the normal numbers, at least 100 times as many cells from these treated donors, compared to untreated rats, are required to produce an equivalent increase in survival of busulphan lethally treated recipients. In contrast, aminochlorambucil, despite producing a marked fall in bone marrow cellularity, has no effect on the number of CFU/femur, yet the marrow from these aminochlorambucil treated donors is no more effective in increasing the survival of busulphan lethally treated recipients than untreated marrow. Theories which may explain this apparent discrepancy and evidence which it affords on the mode of action of busulphan are discussed.
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    A dedication by Vittorio Defendi, Editor (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 235-236 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040760302
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    A kinetic study of the genetic control of hemopoietic progenitor cells assayed in culture and In vivoThis research was supported by the Medical Research Council grant MBT-1420, the Defence Research Board grant 9350-14 G and C and the National Cancer Institute of Canada. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 267-274 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The kinetics of growth of bone marrow cells from normal or genetically anemic mice (Sl/Sld and W/Wv) were studied in irradiated normal and genetically anemic hosts. The parameters followed included total cellularity, the number of peroxidase positive cells, and the number of cells capable of forming colonies in vivo (CFU-S) or in culture (CFU-C). The results of these experiments demonstrate that W and Sl defects alter the growth of CFU-C and peroxidase-positive cells to a modest degree; that the defects are more obvious when studied in spleen rather than in bone marrow; and that there is no additivity of W and Sl defects.Nineteen irradiated recipients of marrow from W/Wv mice were studied after three to six months. Of these, 18 showed host-type erythrocytes, while in one mouse the erythrocytes had the size distribution of W/Wv cells. This finding indicated that occasionally genetically defective stem cells may repopulate irradiated hosts.
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    Induction of fatty acid synthesis in cultured mammalian cells: Effects of cycloheximide and x-rays (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 341-351 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: It was found that L cells deprived of exogenous fatty acids responded adaptively by increased synthesis of fatty acids. This adaptive increase was found to be sensitive to cycloheximide presented at the time of removal of exogenous lipids, but was not sensitive to cycloheximide added six hours after removal of lipids. It was concluded that the adaptive increase in fatty acid synthesis represents a case of enzyme induction. The effect of x-rays on this process was studied. It was found that induction was inhibited to a greater extent than total protein synthesis. Factors of potential importance to such an effect are discussed.
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    Teratology. Journal of Abnormal Development. The Official Publication of the Teratology Society (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 1-2 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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    Variation in the latency of acid phosphatase and ribonuclease throughout the growth cycle of Tetrahymena pyriformis (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 17-22 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The latency of both acid phosphatase and ribonuclease was studied throughout the growth cycle of the ciliate protozoan Tetrahymena pyriformis and was found to be remarkably low in cells in the logarithmic phase of growth. The latency increased progressively throughout the log phase until it reached a maximum just after the cells had entered the stationary phase.The specific activity of acid phosphatase remained constant throughout the whole of the growth cycle while that of ribonuclease decreased as the cells began to leave log phase.The possibility is discussed that all rapidly dividing cells have a high proportion of their acid hydrolases outside the lysosomes and that these free hydrolases may have a function in cell division.
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    Studies on colony formation in vitro by mouse bone marrow cells. II. Action of colony stimulating factorThis work was supported by the Carden Fellowship Fund of the Anti-Cancer Council of Victoria. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 89-99 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An analysis was made of some of the processes involved in the stimulation by colony stimulating factor (CSF) of cluster and colony formation by mouse bone marrow cells in agar cultures in vitro. Colony formation was shown to be related to the concentration and not the total amount of CSF. The concentration of CSF determined the rate of new cluster initiation in cultures and the rate of growth of individual clusters. Colony growth depleted the medium of CSF suggesting that colony cells may utilise CSF during proliferation. Bone marrow cells incubated in agar in the absence of CSF rapidly died or lost their capacity to proliferate and form clusters or colonies. CSF appears (a) to be necessary for survival of cluster-and colony-forming cells or for survival of their proliferative potential, (b) to shorten the lag period before individual cells commence proliferation and (c) to increase the growth rate of individual clusters and colonies.
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    Hemopoietic stem cell distribution in tissues of fetal and newborn miceThis work is supported in part by NIH grant AM 13148, the Fondation pour la Recherche Médicale Française, and the Ligue Nationale Française Contre le Cancer. We thank the staff on the Department of Nuclear Medicine (UCLA) for the permission to use their Co60 radiation facilities. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 107-115 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The variations in the distribution of hemopoietic stem cells in fetal tissues are concomitant with changes in the electrophoretic pattern of hemoglobin. The data presented here are consistent with the hypothesis that the embryonic stem cells can be transformed into adult stem cells in the liver of 15 to 16 days old fetuses.
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    Further studies on the differentiation of a cell line of myeloid leukemia (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 175-184 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A limited time of contact with a conditioned medium from embryo cells induced phagocytotic activity in a cell line of myeloid leukemia followed by the loss of colony forming and leukemogenic capacity. After two days in a high concentration of the conditioned medium, the colonies showed morphological changes which indicated the differentiation of this line of cells. The differentiation-stimulating factor present in the conditioned medium was relatively thermolabile, while the growth-stimulating factor was highly thermostable. Both factors could pass through a dialysis membrane.
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    Correlation of adenosine phosphate levels with cellular morphology in Dictyostelium discoideum amoebae (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 191-196 
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    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Prior to completion of aggregation and the beginning of multicellular differentiation, the amoebae of Dictyostelium discoideum assume two distinct phases with characteristic changes in cellular movement, shape and adhesiveness. These two phases of amoeboid behaviour have been studied with respect to the quantitative analysis of the intracellular adenosine phosphates, using both enzymatic and chromatographic techniques. A higher intracellular ATP level and energy-charge has been found for the actively moving, non-adhesive amoebae as compared to the flattened, mutually adhesive cells. The importance and possible role of ATP in regulating amoeboid form, movement and cell adhesion is discussed.
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    Masthead (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970) 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040760301
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    A brief chronicle on Harry Eagle (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 241-252 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040760304
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    SDS-acrylamide gel electrophoresis and its application to the proteins of poliovirus-and adenovirus-infected human cells (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 273-287 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Sensitive techniques for acrylamide gel electrophoretic analysis have been applied to animal virus systems and have proven generally useful. Estimates of the number of kinds, molecular weights and number of molecules of proteins in almost any biological sample have been made with ease. As applied to the poliovirus-HeLa cell system they reveal four major proteins in the virion and at least ten additional proteins in the infected cell. Some of the intracellular and particulate proteins undergo cleavage reactions following a unique translation in which the genome is apparently translated in toto as one large polypeptide of molecular weight greater than 200,000 daltons. The splits occur at three levels: (a) during synthesis; (b) at intermediate stages; and (c) co-incident with maturation. In vitro studies on protein synthesis, RNA synthesis and virus assembly have substantiated and extended the in vivo observations. The structure of the adenovirion has been established in detail. Hexon, penton base, fiber and core polypeptides and certain relevant subviral structures have been identified. Nearly all of the proteins synthesized in the infected cells after 20 hours are viral. The major structural antigens (hexon and penton) predominate and are made in 10 to 50 fold excess but the internal core polypeptides are not produced in great excess. Studies on the synthesis of polypeptides and their assembly into morphological subunits and virions show that hexon and penton polypeptides are made in about four and two minutes respectively on cytoplasmic polyribosomes, that morphological subunits are formed within five minutes of synthesis of protein, and that there is a delay of greater than one half hour for entry of hexons into virions.
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    The molecular biology of reovirusDedicated to Dr. Harry Eagle on the occasion of his 65th birthday. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 289-301 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The genome of reoviruses consists of ten discrete segments of double-stranded RNA. The molecular weight of three of these, the L segments, is about 2.7 × 106; of another three, the M segments, about 1.4 × 106; and of the remaining four, the S segments, 0.6-0.8 × 106. In addition, reovirions contain about 2,000 molecules of oligonucleotide from 2 to 20 nucleotides long, most of which are very rich in ademine.The protein capsid of reovirions, which consists of an inner core and 92 outer shell capsomeres, is composed of seven species of polypeptides which also fall into three size classes. The size of six of these polypeptides is such that each accounts for the entire information content of one of the genome RNA segments.Reovirus messenger RNA molecules are transcripts of all ten genome RNA segments. Transcription proceeds not only in vivo in the infected cell, but can also take place in vitro. It is then catalyzed by reovirus cores, the polypeptide composition of which is strikingly similar to that of E. coli DNA-dependent RNA polymerase. Transcription both in vitro and in vivo is entirely asymmetric and is conservative with respect to template.The patterns of transcription of the several reovirus genome RNA segments have been determined both in vitro and in vivo and the pattern of translation has been analyzed under in vivo conditions. The results suggest that transcription and especially translation are regulated within infected cells.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/jcp.1040760308
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    The distribution of rapidly hybridizing RNA sequences in heterogeneous nuclear RNA and mRNA from hela cellsThis work was supported by grants from the National Institutes of Health (R01 CA 11159-02), the National Science Foundation (GB 8497) and the American Cancer Society. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 349-356 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Both HnRNA and mRNA contain sequences derived from DNA which is reiterated many times in the cell genome. The degree of reiteration for sites binding HnRNA appears to be greater than for those DNA sites which bind mRNA.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/jcp.1040760312
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    Differential temperature sensitivity of normal and cancer cells in culture (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 373-379 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Serially-propagated growing heteroploid and growing diploid cell cultures do not survive incubation at 42° for 24 hours, whereas contact-inhibited diploid monolayers are still viable after at least nine days at this elevated temperature. Heat-treated heteroploid HeLa cells and growing diploid cells exhibit a variety of morphologic abnormalities, but contact-inhibited cells are only minimally affected. A similar differential temperature sensitivity exists in the synthesis of cellular macromolecular components such as DNA, RNA, and protein: incorporation of radioactive precursors is drastically reduced in growing diploid and heteroploid cells after 24 hours at 42°, but not in contact-inhibited cells. Incorporation of labelled glucose, choline, or linolenic acid is actually enhanced in heat-treated contact-inhibited cells.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/jcp.1040760315
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    Chromium(III) Complexes with Benzoylacetanilide and N-benzoylphenylhydroxylamine (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 954-957 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Tab.
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    URL: http://dx.doi.org/10.1002/prac.19703120528
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    Substituted Piperazino-Ureas as possible central nervous system depressants (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 964-967 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Tab.
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    URL: http://dx.doi.org/10.1002/prac.19703120530
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    Studies in mixed aqueous media. I. Acidity Constants of Isomeric Chloro-o-toluidinium Ions in the system ethanol-water (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 977-988 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Acidity constants of 3, 4, 5 and 6-chloro-o-toluidinium ions are measured at 25°C in aqueous-ethanol solvents containing 0, 20, 35, 50, 65 and 80 wt% of ethanol by pH metry. The correction factor giving stoichiometric significance to pH meter readings have been evaluated for three different acid concentrations in each of the above mentioned solvent systems. Attempt has been made to correlate the strength of each of the anilinium ions with various solvent properties. HAMMETT-type relations have been derived for correlating acid strengths with structural properties.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/prac.19703120602
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    Über Benzofuro-benzthiazole. II. Die Struktur des bei der Oxydation von 3-Thiobenzoylamino-dibenzofurans isolierten Benzofurobenzthiazols (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 1007-1010 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Vom 2-Brom-3-thiobenzoylamino-dibenzofuran 1 d ausgehend, wurden 2-Phenyl-4-brom-benzofuro[2,3-g]benzthiazol 3a und 2-Phenyl-benzofuro[2,3-f]benzthiazol 2 erhalten. Durch Vergleichen der Eigenschaften (IR-, UV-Spektren u.a.) der Substanzen 2 und 3a mit denjenigen des Benzofuro-benzthiazols, das durch Oxydation des 3-Thiobenzoylamino-dibenzofurans 1a erhalten wurde, wird die angulare Struktur 3 dieser Verbindung bewiesen.
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    URL: http://dx.doi.org/10.1002/prac.19703120605
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    Struktur und Reaktivität aliphatischer Diazoverbindungen. XIV.Auszugsweise vorgetragen am 7. 5. 1969 anläßlich der Chemiedozenten-Tagung in Rostock. Elektrochemische Oxydation von α-Phenyl-α-Diazocarbonyl-Verbindungen zu Diphenyl-Derivaten (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 105-116 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die elektrochemische Oxydation der α-Aryl-α-diazocarbonyl-Verbindungen 4a-n an der rotierenden Platin-Scheibenelektrode in LiClO4/Acetonitril führt im Primärschritt zu Diazonium-Radikalionen 5. Die anodischen Halbstufenpotentiale dieser Elektrodenreaktion liegen in Abhängigkeit von der Struktur der Diazoverbindung bei E1/2 = + 1,36 V bis +1,76 V (GKE). Die unter Verwendung der gleichen Elektrode aufgenommenen KALOUSEK-Umschaltkurven und Oszillopolarogramme der Diazoverbindungen vom Strukturtyp C6H5—CN2—CO—R2 geben charakteristische Hinweise für eine rasche Folgereaktion von 5 unter Bildung reversibel oxydierbarer Produkte (E1/2 = +1,23V bis +1,29V (GKE)). Durch Isolierung von Diphenyl-4,4′-dicarbonsäure 15 aus dem Produktgemisch der Elektrolyse von Phenyl-benzoyl-diazomethan 4c und Phenyl-diazoessigsäure-äthylester 4k wird nachgewiesen, daß die Diazonium-Radikalionen 5 zur Dimerisierung unter Bildung von Diphenyl-Derivaten befähigt sind. Substitution des Phenylrestes in p-Stellung zum Diazo-Kohlenstoffatom verhindert diese oxydative C—C-Verknüpfung. Der Einfluß dieser Folgereaktion auf das polarographische Verhalten der α-Diazocarbonyl-Verbindungen wird diskutiert und der Mechanismus der Bildung weiterer Elektrolyseprodukte erläutert.
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    URL: http://dx.doi.org/10.1002/prac.19703120114
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    Darstellung und reaktives Verhalten von Lithiumsulfinamiden (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 129-134 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: p-Toluolsulfinamid 1 und N-Isopropyl-p-toluolsulfinamid 2 reagieren in ätherischer Lösung mit Lithiumbutyl zu Lithiumsulfinamiden 1a, 2a, die zur Darstellung N-substituierter bzw. N,N-disubstituierter Sulfinamide geeignet sind.
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    URL: http://dx.doi.org/10.1002/prac.19703120116
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    Struktur und Reaktivität aliphatischer Diazoverbindungen. XV. Über die Strukturabhängigkeit der anodischen Halbstufenpotentiale aliphatischer Diazoverbindungen und ihre Korrelation mit den Geschwindigkeitskonstanten der säurekatalysierten Hydrolyse und Thermolyse (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 117-128 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Der Substituenteneinfluß auf die Verschiebung der polarographischen Halbstufenpotentiale der reversiblen Oxydation von Aryl-aroyl-diazomethanen 3 und 4, Aryl-diazo-essigsäureäthylester 5, Diaryl-diazomethanen 6 und p-Nitrophenyl-diazomethan-Derivaten 7 zu Diazonium-Radikalionen 2 in 0,1 M LiClO4/Acetonitril an der rotierenden Platin-Scheibenelektrode läßt sich durch modifizierte HAMMETT- bzw. TAFT-Gleichungen beschreiben. Der Zusammenhang zwischen der Struktur der Diazoverbindungen, der Stabilität der Diazonium-Radikalionen, der Lage der E1/2-Werte und der Größe der Reaktions-konstanten ϱ wird diskutiert. Die anodischen Halbstufenpotentiale stehen in linearer Beziehung zu den Geschwindigkeitskonstanten homogener chemischer Reaktionen von Diazoverbindungen an der polarographisch aktiven Gruppe, unabhängig vom Reaktionsmechanismus und der Natur der Reaktionsprodukte. Bei der säurekatalysierten Hydrolyse der p-Nitrobenzoyl-diazoalkane 9 hat dieser lineare Zusammenhang auch dann Gültigkeit, wenn weder die unter dem Einfluß von Substituenten auftretenden Änderungen ΔE1/2 noch Δ1g k durch ß-, ß*- bzw. Es-Konstanten beschreibbar sind. Es werden die Gründe diskutiert, warum für aliphatische Diazoverbindungen keine universell anwendbare lineare Freie-Energie-Beziehung Δ1g k = f(ΔE1/2) für den Zusammenhang zwischen elektrochemischen und kinetischen Parametern existiert.
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    Beiträge zur Chemie der Phosphinalkylene. VI. Dimethylamino-methylen-phosphorane (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 135-144 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Dimethylamino-methylen-phosphorane des Typs 1. 2 und 3 reagieren mit CIPR2 zu phosphinosubstituierten Alkylidenphosphoranen 4 - 8 (R=Me, Et, OEt, NMe2), 9, 10 (R=Et, OEt) und 11, 12 (R=Et, OEt). Unter Verwendung von CH3PCl2 und PCl3 liefert 1 das „Bis-Ylen“ 13 und das „Tris-Ylen“ 14. 4, 13 und 14 werden durch Methyljodid in die mesomeriestabilisierten Ylid-Quartärsalze 15-17 überführt. Aus 15 und 16 lassen sich mit Hilfe von NaNH2 in fl. NH3 die Ylide 18 und 19 gewinnen. Ihre Struktur ist durch die Wittig-Reaktion mit Acetaldehyd gesichert. Zur näheren Charakterisierung werden die phosphinosubstituierten Alkylidenphosphorane mit ätherischem Chlorwasserstoff in die entsprechenden Phosphoniumsalze 20-27 überführt.
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    URL: http://dx.doi.org/10.1002/prac.19703120117
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    Über ein ungewöhnliches O-Nucleosid-O2,5′-(α-D-Methyl-2-desoxyribofuranosido)-thymin (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 145-149 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Es wird über die Strukturaufklärung von zwei Reaktionsprodukten berichtet, die neben anderen beim Umsatz von 5′-O-Tosyl-thymidin 1 mit KF · 2H2O in Methanol entstehen. Danach handelt es sich um 5′-O-Methyl-thymidin und um O2 · 5′-(α-D-Methyl-2-desoxy-ribofuranosido)-thymin 3. Die letztgenannte Verbindung entsteht durch eine ungewöhnliche Reaktion aus O2,5′-Cyclo-thymidin 2 und stellt das erste Beispiel eines „umgekehrten“ Pyrimidin-O-Nucleosids dar. 3 reagiert mit Diazomethan zu drei im Heterocyclus mono-methylierten Produkten.
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    URL: http://dx.doi.org/10.1002/prac.19703120118
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    Synthesis and Anticonvulsant Properties of some 4-ThiazolidonesThis investigation was supported by a research grant from the Indian Council of Medical Research, New Delhi, India. (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 201-204 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Several 3-furfuryl-4-oxo-thiazolin-2-yl-substituted hydrazones have been synthesized and tested for their anticonvulsant activity.
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    Masthead (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970) 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120201
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    Beitrag zur Thermodynamik des Mischungssystems Cyclohexanon - Cyclohexanol und zur Kenntnis azeotroper Verunreinigungen des Cyclohexanons (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 214-220 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Aus Dampf  -  Flüssigkeit-Gleichgewichten und aus den Mischungsenthalpien H0,5E = 387,5 cal/Mol bei 80,30° und 312,0 cal/Mol bei 26,94° wurden im Temperaturbereich 110° bis 0° die Exzeßgrößen des Systems angenähert bestimmt. GE, HE, TSE und das bei 30° und 50° ebenfalls gemessene VE sind positiv und nahezu symmetrisch zum Molenbruch 0,5. Die von etwa 20 cal/Mol bei 110° auf 116 cal/Mol bei 0° ansteigende freie Exzeßenthalpie ist zu klein, um ein Azeotrop zu bewirken, was auch direkt durch Rayleigh-Destillation nachgewiesen wurde.Reines Cyclohexanon in Glasampullen erleidet eine Kondensation, die zu niedrigen Temperaturen hin nur langsam abklingt. Sie wurde kryoskopisch nachgewiesen und dabei die kryoskopische Konstante des Cyclohexanons zu -46,7° bestimmt. Als Kondensationsprodukt wurde auf Grund des I.R.-Spektrogramms eindeutig 1-[Cyclohexen(1)-yl]cyclohexanon(2) gefunden.
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    Synthesis of heterocyclic-substituted Furochromones and Allied Compounds (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 228-239 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The preparation of a number of new 7-heterocyclic-substituted furochromones and related chalcones is reported. Oxidation of the chalcones, by alkaline hydrogen peroxide, leads to the formation of derivatives of aurone.Chemical, analytical and spectroscopic results are in accord with the assigned structures.
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    Darstellung von Acetondicarbonsäure-di-tert.-butyl-sowie-dibenzylester (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 240-244 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Der Acetondicarbonsäure-di-tert.-butylester 3 wird über das Di-silbersalz 4, bzw. das Di-säurechlorid 2 der Acetondicarbonsäure 1 erhalten, während der Dibenzylester 6 durch Umesterung aus ihrem Dimethylester zugänglich ist.
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    Versuche zur Synthese von Derivaten der O-Methylmandelsäure (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 284-291 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Chlormethoxyessigsäure-methylester 1 reagiert wie mit Aromaten auch mit aromatischen Heterocyclen, DVB-vernetztem Polystyrol bzw. geeignet substituierten Phenolen unter Bildung der entsprechenden analogen Derivate von O-Methylmandelsäure- oder Diarylessigsäureester.  -  Phenolester von O-Methylmandelsäuren sind nur aus den entsprechenden Säurechloriden darstellbar. Bei Versuchen zur Umesterung der Methylester mit Phenolen erhält man unter Kondensation Lactone der o-Hydroxyphenylessigsäure. - Aus 1,2,2,2-Tetrachloräthyl-methyläther 11 und Aromaten sind u.a. 2′,2′,2′-Trichlor-1′-methoxyäthylbenzole 12 zugänglich, die entgegen Angaben der Literatur nicht zu den erwarteten O-Methylmandelsäurederivaten hydrolysierbar sind. Mit K-tert.-Butylat erhält man aus 12a 2′,2′-Dichlor-1′-methoxystyrol, dessen Hydrolyse zu 2′,2′-Dichloracetophenon führt.
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    NMR-Untersuchungen zur Konformation der 2-Methylen-cycloalkanone-(1) (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 292-299 
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    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die 2-Methylen-cycloalkanone-(1) der Ringgröße C5—C8, C10—C13 und C16 wurden NMR-spektroskopisch unter Verwendung der Methode der Lösungsmittelverschiebung untersucht. Dabei konnten im Gegensatz zu früheren Untersuchungen genauere Aussagen zur Vorzugskonformation der exo-Methylen-Ketogruppierung gemacht werden. Für den Fünfring ergab sich eine fixierte planare s-cis-Konformation; für die Ringe der Größe C6—C8 wurden tordierte s-cis-Formen festgestellt. Im Zehnring liegt eine fixierte nichtplanare s-trans-Konformation vor, und bei den höheren Ringen der Größe C11—C13 und C16 ist ein gewisser Prozentsatz von s-cis-Form für die beobachteten Lösungsmittelverschiebungen verantwortlich. Weiterhin wird ein Vergleich der experimentell gemessenen chemischen Verschiebungen der exo-Methylenprotonen obengenannter Verbindungen mit denen aus einer semiempirischen Beziehung berechneten vorgenommen.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/prac.19703120214
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    Zur kationischen Polymerisation von Stilbenen mit SbCl5 (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 300-306 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Für die kationische Polymerisation von trans-Stilben und p-substituierten trans-Stilbenen wird die Kinetik der Reaktion mit Hilfe eines anisothermen Kalorimeters bestimmt und das Geschwindigkeitsgesetz ermittelt. Die Struktur der erhaltenen Polystilbene wird auf Grund der DTG-Kurven, der Pyrolysegaschromatographie, der Molekulargewichte und spektroskopischer Vergleiche diskutiert.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120215
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  • 189
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    Acylhalogenosen der 3-Desoxy-D-galaktose (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 314-317 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Durch Acylieren der 3-Desoxy-D-galaktose mit Benzoylchlorid oder mit Toluylchlorid in Pyridin erhält man die Tetra-O-benzoyl- (1a, 3a) bzw. die Tetra-O-p-toluyl-3-desoxy-α,β-D-galaktopyranose (1b, 3b). Daraus lassen sich durch Behandeln mit Chlorwasserstoff oder mit Bromwasserstoff die Benzochlor-(2a) bzw. die Toluobrom-3-desoxy-α-D-galaktopyranose (2b) darstellen. Ihre Pyranose-Strukturen gehen aus der Überführung in das bekannte Methyl-3-desoxy-β-D-galaktopyranosid hervor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120217
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  • 190
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    7- und 8-Ringverbindungen. XII. Konfigurations- und Konformationszuordnung der Cis/trans-isomeren tert.-Butylcycloheptanole (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 318-325 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An den 6 cis/trans-isomeren 2-, 3- und 4-tert.-Butylcycloheptanolen wird der Zusammenhang zwischen Konfiguration und Konformation auf theoretischem Wege bestimmt. Danach und in Übereinstimmung mit Konfigurations- und Konformationsuntersuchungen durch IR-Spektroskopie, mit Hilfe der BARTONschen Regeln und der Äquilibrierungsgleichgewichte zeigt sich, daß bei den 2- und 4-tert.-Butylcycloheptanolen der Anteil der Konformationen mit quasi-axialer Hydroxylanordnung im cis- größer als im trans-Isomeren ist. In den 3-tert.-Butylcycloheptanolen ist dagegen in der trans-Konfiguration die quasi-axiale Hydroxylanordnung stärker als in der cis-Konfiguration vertreten. Die AUWERS-SKITA-Regel erweist sich als nicht geeignet zur Konfigurationszuordnung.
    Additional Material: 5 Tab.
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    URL: http://dx.doi.org/10.1002/prac.19703120218
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  • 191
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    Reaktionen mit Diazoketonen. X. Synthese von 1,2,3-Triaroyl-cyclopropanen und 3,4,5-Triaroyl-Δ2-pyrazolinenHerrn Prof. Dr. L. Reichel zum 70. Geburtstag zugeeignet (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 394-396 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120228
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  • 192
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    Über α-substituierte Phosphonsäureester. X. Synthese von α-Chlor-α-methoxy-methanphosphonsäureestern und α-Chlor-α-methylthio-methanphosphonsäureestern (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 475-482 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Durch Chlorierung von Alkoxy- bzw. Alkylthio-methanphosphonsäureestern oder durch Umsetzung von Dichlormethylmethyläther bzw. -sulfid mit Phosphorigsäuretriäthylestern bzw. mit Phosphorigsäurediäthylester-Na wurden α-Chlor-α-methoxy- bzw. α-Chlor-α-methylthio-methanphosphonsäureester 4 dargestellt.Durch Umsetzung des Thioderivates 4 d mit Phosphorigsäureestern konnte der Methyl-thiomethan-bis-phosphonsäuretetraäthylester gewonnen werden, der u. a. auch aus Dichlormethylmethylsulfid analog in einer Synthesestufe zugänglich war. - Nach den beschriebenen Verfahren wurde schließlich α,α-Dichlor-α-methylthio-methanphosphonsäurediäthylester 7, bzw. das analoge Phenoxyderivat 9 synthetisiert.
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    URL: http://dx.doi.org/10.1002/prac.19703120312
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  • 193
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    Synthese von kristallin-flüssigen Verbindungen. III. n-Alkyl- und n-Alkoxyderivate des 2,5-Diphenyl-pyrimidins (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 494-506 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Es werden die Synthesen des 2,5-Diphenyl-pyrimidins und der p,p′-disubstituierten 2,5-Diphenyl-pyrimidine mit n-Alkyl- und n-Alkoxyresten als Flügelgruppen beschrieben. Das kristallin-flüssige Schmelzverhalten der Glieder der beiden homologen Reihen wird diskutiert.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120314
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  • 194
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    Steroide und steroidähnliche Verbindungen. I. Synthese von 9β-Hydroxy-1β.5β-dimethyl-4β-(p-methoxyphenyl)-trans-bicyclo[4.3.0]nonanAlle in dieser Arbeit beschriebenen Bicyclo[4,3,0]nonan-Derivate sind Racemate. In den Bezeichnungen und Formeln wurde jeweils nur das Enantiomere erfaßt, das an C - 1 die gleiche absolute Konfiguration hat wie C - 13 des Östrons. Die Buchstaben α und β wurden zur Konfigurationsbezeichnung in gleicher Weise verwendet wie in der Steroidnomenklatur. (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 483-493 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Ausgehend von p-Methoxypropiophenon, Vinylmagnesiumbromid und 2-Methylcyclopentandion-(1,3) wird 9β-Hydroxy-1β,5-dimethyl-4-(p-methoxyphenyl)-bicyclo[4,3,0]nonadien-(4,6) 7 a synthetisiert. Durch NMR-Spektroskopie wird bewiesen, daß die Hydrierung dieser Verbindung zum trans-Bicyclo[4,3,0]nonan-System führt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120313
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    Über die Koordinationstendenz saurer Aminogruppen. VIIVI. Mitteilung: H. Hennig, R. Daute, E. Hoyer, Vortragsberichte zum Symposium über Koordinationschemie der Übergangselemente, Jena 1969, Sektion C, S. 70.. 5,7-Dinitro-8-amino-chinolin-ein ampholytisches Ligandensystem (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 554-560 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die Komplexbildungstendenz von 5,7-Dinitro-8-amino-chinolin (DNAC—H) gegenüber verschiedenen 3d-Metallionen (Cu2+, Pd2+, Zn2+, Ni2+, Co3+ und Cr3+) wird beschrieben und das komplexchemische Verhalten von DNAC—H im Vergleich zu 7-Acetyl-8-amino-chinolin (AAC—H) und zu 8-Amino-chinolin (AC—H) diskutiert. DNAC—H liegt  -  bedingt durch die -I-Substitution  -  als ampholytisches Ligandensystem vor, so daß mit den genannten Metallionen ausschließlich Neutralchelate gebildet werden. Die spektralen und magnetischen Eigenschaften der dargestellten Metallchelate, sowie der Einfluß der koordinierten Metallionen auf die Lage der Halbstufenpotentiale der Ligand-Nitrogruppen werden diskutiert.
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    URL: http://dx.doi.org/10.1002/prac.19703120403
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  • 196
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    Methyläther der 3-Desoxy-D-galaktose (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 635-640 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Aus Methyl-3-desoxy-D-galaktopyranosid werden 2,4,6-Tri-O-methyl-, 2,4-Di-O-methyl- und 4,6-Di-O-methyl-3-desoxy-D-galaktose synthetisiert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120413
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  • 197
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    Relaissynthesen von Heterocyclen. VIII. Synthese und Ringspaltung von s-Triazolo[3,4-f]1,2,4-triazinen (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 669-682 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die Acylierung von s-Triazolyl-amidinen 1 führt zu N-[1,2,4-Triazolyl-(4)]-N′-carbalkoxy-amidinen 2, die durch Kochen in Äthylglykol oder Phenol zu s-Triazolo[3,4-f]as-triazinonen-(8) 3 cyclisieren. Die analoge Umsetzung der Verbindungen 1 mit Orthocarbonsäureestern ergibt unter direktem Ringschluß ohne Bildung isolierbarer offenkettiger Acylierungs-produkte die s-Triazolo[3,4-f]as-triazine 5. 6,8-Disubstituierte s-Triazolo[3,4-f]as-triazine sind auch durch Umsetzung von 4-Arylidenamino-1,2,4-triazolen mit Natriumamid zugänglich. Durch Hydrogenolyse dieser Verbindungen entstehen Imidazole. Die Quaternierung und anschließende Hydrolyse liefert bisher unbekannte 6-substituierte 1,2,4-Triazine.
    Additional Material: 5 Tab.
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    URL: http://dx.doi.org/10.1002/prac.19703120417
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    Chemie des Dimethylsulfoxids. V [1]. Chlorierung und Bromierung von Dimethylsulfoxid (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 683-689 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Methyl-chlormethylsulfoxid ist in 60proz. Ausbeute durch Direktchlorierung von DMSO in Gegenwart von Triäthylamin erhältlich. Die Weiterchlorierung von Methyl-chlormethylsulfoxid liefert Methyl-trichlormethylsulfoxid. Bei der Einwirkung von elementarem Brom auf DMSO entsteht Trimethylsulfonium-bromid als Hauptprodukt neben Dimethylsulfid, Methansulfonsäure und Polyoxymethylen. Der Mechanismus der Bromierungsreaktion wird beschrieben.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120418
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  • 199
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    Über Inhaltsstoffe der Torffulvosäurefraktion (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 690-700 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Durch Heißwasserextraktion isolierte Torffulvosäuren lassen sich an aktiviertem Wofatit E mit verschiedenen Lösungsmitteln fraktionieren. Im wäßrigen Perkolat sind papier- und dünnschichtchromatographisch verschiedene Monosaccharide und Aminosäuren nachweisbar.Als Ligninspaltstücke wurden im Acetoneluat p-Hydroxybenzaldehyd, Vanillin und 3,4-Dihydroxypropiophenon gefunden. Daneben konnten auch andere Pflanzeninhaltsstoffe oder -abbauprodukte wie Glucoacetovanillon, 2,4,6-Trihydroxyacetophenon, 4-Hydroxy-2-methoxyacetophenon und 7,8-Dihydroxycumarin identifiziert werden. Ihre Beteiligung an der Huminsäuregenese ist über verschiedene Reaktionswege möglich.
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    URL: http://dx.doi.org/10.1002/prac.19703120419
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    Spektroskopisches Verhalten von organischen Schwefelverbindungen. IV [1]. Lage, Form und integrale Intensität der SO2-Valenzschwingungsbanden einfacher Sulfone (1970)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 312 (1970), S. 701-711 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An 40 Dialkyl-, Benzylalkyl- und Phenylalkylsulfonen werden die Einflüsse der Molekülreste und verschiedener Lösungsmittel auf die SO2-Valenzschwingungen untersucht. Induktive und mesomere Effekte wirken vorwiegend auf die symmetrische Schwingung, sterische Effekte und Lösungsmittel auf die antisymmetrische. Die integrale Absorptionsintensität der symmetrischen Schwingung alterniert mit zunehmender Länge der Alkylreste, die Intensität der antisymmetrischen Schwingung nimmt erst stark ab und nähert sich dann langsam einem für jede homologe Reihe verschiedenen Grenzwert. Lösungsmittel beeinflussen die integrale Intensität beider Schwingungen nur wenig.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19703120420
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