Publication Date:
2020
Description:
〈p〉Publication date: Available online 21 January 2020〈/p〉
〈p〉〈b〉Source:〈/b〉 Gene〈/p〉
〈p〉Author(s): Roghayeh Pourbagher, Haleh Akhavan-Niaki, Seyed Gholam Ali Jorsaraei, Sadegh Fattahi, Davood Sabour, Ebrahim Zabihi, Zeinab Abedian, Masoumeh Ghasemi, Monireh Golpour, Amrollah Mostafazadeh〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Down-regulation of stemness genes expression is important in differentiation therapy against cancer stem cells (CSCs). The aim of this study was to evaluate the 〈em〉Oct4〈/em〉 , 〈em〉Sox2〈/em〉, 〈em〉Nanog〈/em〉, and 〈em〉C-myc〈/em〉 expression in rat breast cancer stem cells (LA7) which treated with human ovarian follicular fluid (FF), replicative senescent fibroblast culture supernatant (P14), and 16 h serum starved fibroblast supernatant (16h-SFS). The cells were exposed to these biological fluids for 24 h, 72 h, and 7 days. Stem-loop RT-qPCR assay was used to quantify the expression of above mentioned genes. Results showed that FF had the least cytotoxic effect on the LA7 cells. Except for 〈em〉Nanog〈/em〉 gene, exposure of LA7 cell line to 16h-SFS and P14 decreased significantly expression of the three other genes after 24 h (P〈0.05). 〈em〉Nanog〈/em〉 and 〈em〉Sox2〈/em〉 genes expression was also decreased in LA7 cells which have been already treated with FF for 24 h. Moreover, compared to the control solution, the expression of 〈em〉Oct4〈/em〉 increased significantly after 7 days exposure to FF (P〈0.05). Annexin V-PE /7-AAD-, acridine orange/ethidium bromide staining and doubling time assays revealed apoptosis and necrosis induction by these biological fluids in LA7 cells. Moreover, in an 〈em〉in vitro〈/em〉 model of metastasis assay, i.e., scratch test, these fluids exhibited anti-LA7 migration activity which culminated in 16 h-SFS treated cells. Generally, this study showed that FF, 16h-SFS, and P14 have positive effects on down-regulation of 〈em〉Nanog〈/em〉, 〈em〉Oct4, Sox2〈/em〉 and 〈em〉C-myc〈/em〉 expression, and consequently can increase the differentiation of breast cancer stem cells. For the first time, this study provided some evidence indicating that some biological fluids have potential to differentiate the CSCs, show anti- survival, growth-, and cell migration activity.〈/p〉〈/div〉
〈/div〉
Print ISSN:
0378-1119
Electronic ISSN:
1879-0038
Topics:
Biology
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