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  • Articles  (62)
  • Articles: DFG German National Licenses  (62)
  • Analytical Chemistry and Spectroscopy
  • Biochemistry and Biotechnology
  • Brassica napus
  • General Chemistry
  • Inorganic Chemistry
  • Life and Medical Sciences
  • Springer  (62)
  • 1985-1989  (58)
  • 1955-1959  (4)
Collection
  • Articles  (62)
Source
  • Articles: DFG German National Licenses  (62)
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  • 1
    Electronic Resource
    Electronic Resource
    Evidence for spontaneous diploid androgenesis in Brassica napus L. (1989)
    Springer
    Sexual plant reproduction 2 (1989), S. 15-17 
    Details
    ISSN: 1432-2145
    Keywords: Brassica napus ; Spontaneous androgenesis ; Erucic acid content ; Flower colour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seeds of androgenetic origin were obtained among the F1 progenies of two crosses between resynthesized and cultivated forms of Brassica napus. The high-erucic, white-flowered, resynthesized line No7076 acted as the female, and the zero-erucic, yellow-flowered, cultivars ‘Topas’ and ‘Puma’, as males. No androgenetic seeds were obtained in the reciprocal crosses. Resynthesized rape could thus be of potential use for the production of androgenetic plants. Of special interest is the high frequency (21%) of spontaneous androgenesis observed in one of the two crosses. One plant, determined from erucic acid content and flower colour analysis as androgenetic, had a diploid chromosome number. Further knowledge about the genetic control of spontaneous androgenesis in the present material and the origin of the cytoplasm in androgenetic plants are required in order to exploit this phenomenon in practical plant breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00190114
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative analysis of stamen polypeptides of a rapeseed cultivar, Regent, a CMS polima line, and temperature-restored male-fertile polima line (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 114-118 
    Details
    ISSN: 1432-2145
    Keywords: Brassica napus ; Cytoplasmic male sterility ; Polypeptides ; Temperature ; Restoration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Polypeptides were extracted from stamens of a rapeseed (Brassica napus) cultivar, Regent, a near isogenic male-sterile line, Polima-R7 (Pol-R7), and a high-temperature-restored malefertile Pol-R7 (TR) and subsequently separated by two-dimensional isoelectric focusing-polyacrylamide gel electrophoresis under denaturing conditions. Four variable polypeptides with a pI around 6 were observed. Two stamen polypeptides (40000 Da, 38000 Da) were unique to Regent, and the other two (32000 Da, 30000 Da) were unique to the male-sterile Pol-R7. When the male-sterile Pol-R7 was treated with day/night temperatures of 30°/24° C for 7–10 days prior to flowering, both polypeptides unique to Regent reappeared, while the smaller polypeptides disappeared. Temperature-restored male-fertile Pol-R7 (TR) produced fertile pollen, while its short stamen filaments resembled those of the male-sterile Pol-R7. These changes in protein expression may be causally related to the CMS phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189270
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  • 3
    Electronic Resource
    Electronic Resource
    Virulence of Agrobacterium tumefaciens strains with Brassica napus and Brassica juncea (1989)
    Springer
    Plant cell reports 8 (1989), S. 303-306 
    Details
    ISSN: 1432-203X
    Keywords: Ti plasmid ; Virulence ; Brassica napus ; Brassica juncea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Brassica napus and Brassica juncea were infected with a number of Agrobacterium tumefaciens strains. Tumourigenesis was very rapid and extremely efficient on B. juncea with all but one of the strains. Tumourigenesis on B. napus varied widely. It was very efficient with the nopaline strains, was reduced with the succinamopine strain A281 and was very weak with the octopine strains. The latter observation was confirmed with six different B. napus rapeseed cultivars. The selectivity was due to differences in the virulence of Ti plasmids with B. napus, rather than the tumourigenicity of the T-DNA or virulence of the chromosomal genes associated with the strains. An exception was strain LBA4404. The virulence of the octopine strains was increased by coinfection with more virulent disarmed strains and by induction with acetosyringone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274136
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 325-329 
    Details
    ISSN: 1432-2242
    Keywords: A. tumefaciens ; A. rhizogenes ; Brassica napus ; Plant development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The response of oilseed rape cultivars to infection with Agrobacterium tumefaciens and A. rhizogenes and the possibility of regenerating genetically transformed oilseed rape plants were examined. The frequency at which Agrobacterium induced galls or hairy-roots on in vitro cultured plants ranged from 10% to 70%, depending on the cultivar. From galls induced by the tumorigenic strain T37, known to be strongly shoot inducing on tobacco, roots developed frequently. Occasionally, shoots formed and some of these produced tumour cell specific nopaline. Attempts to grow the transformed shoots into plants have so far been unsuccessful. Whole plants transformed with Ri-T-DNA, however, were regenerated. These had crinkled leaves and abundant, frequently branching roots that showed reduced geotropism, similar to previously isolated Ri T-DNA transformed tobacco and potato plants. The transformed oilseed rape plants flowered, but failed to form seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252075
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  • 5
    Electronic Resource
    Electronic Resource
    Cytoplasmic male sterility in rapeseed (Brassica napus L.) (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 145-150 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Cytoplasmic male sterility ; Chloroplast DNA ; Mitochondrial DNA ; Restriction patterns
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction patterns of chloroplast (cp) and mitochondrial (mt) DNA in Brassica napus rapeseed reveal the alloplasmic nature of cytoplasmic male sterility in this crop. Both the Shiga and Bronowski systems probably exploit cytoplasmic diversity in B. napus cultivars arising from introgression of cytoplasm from the other rapeseed species, B. campestris. Nuclear genes specific to these systems do not cause sterility in maintainers (Bronowski and Isuzu-natane) because they have a campestris cytoplasm, but give rise to sterility in napus cytoplasms. In the course of hybridization to napus cultivars a line with the triazine resistant cytoplasm (a campestris cytoplasm) has undergone an alteration in the mt genome rendering its restriction pattern more similar than previously to that of napus. The alteration may be an inversion between 7.2 and 3.4 kb in length.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266985
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  • 6
    Electronic Resource
    Electronic Resource
    Artificial Brassica napus flowering in Bangladesh (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 465-468 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Resynthesis ; Adaptation to short day ; Earliness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Natural rapeseed (Brassica napus L.; AACC 2n=38), originated in the temperate climate of the Southwest European Mediterranean region, fails to complete its generative phase in the subtropics and is thus not cultivated in countries like Bangladesh. Adapted agroecotypes are available from the diploid representatives of its genome A (B. campestris/pekinensis, 2n=20) and C (B. oleracea/alboglabra, 2n=18). An artificial resynthesis based on carefully selected progenitor lines was expected to give a photoperiodically better adapted rapeseed. ♀ B. pekinensis x ♂ B. oleracea/alboglabra gave 2 hybrids and 87 matromorphous plants from 1,448 crossed flowers and the reciprocal combination gave no hybrid but 11 matromorphous plants from 2,228 pollinated flowers. The two true hybrids were vegetatively propagated and chromosome doubled. Part of the F2 was grown in Sweden (all plants flowered and the most early ones were selected), part in Bangladesh (13 out of 706 plants flowered). The selected F3 material flowered in Bangladesh and transgressions in earliness could be recorded, some lines were of definite agronomic potential. A correlation in earliness between reaction in Sweden (long day) and Bangladesh (short day) was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262517
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  • 7
    Electronic Resource
    Electronic Resource
    Inheritance patterns of erucic acid content in populations of Brassica napus microspore-derived spontaneous diploids (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 489-494 
    Details
    ISSN: 1432-2242
    Keywords: Microspore culture ; Spontaneous diploids ; Erucic acid ; Inheritance ; Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The levels of erucic acid and other fatty acids in seeds of microspore-derived spontaneous diploid plants from crosses between low and high erucic acid parents were examined. The analysis confirmed that erucic acid is simply inherited and is determined by two genes that act in an additive manner. The effects of the genes for erucic acid on the levels of the other fatty acids was also determined and many significant correlations were found. In particular, erucic acid levels were negatively correlated with oleic acid and linoleic acid levels. The study also illustrates several advantages of using haploidy to analyze the inheritance of agronomically important traits. In particular, the number of phenotypic classes is smaller in androgenic populations and differences between classes are greater than in an F2 population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274268
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  • 8
    Electronic Resource
    Electronic Resource
    Brassica naponigra, a somatic hybrid resistant to Phoma lingam (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 651-656 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Brassica nigra ; Somatic hybrids ; Resistance to Phoma lingam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Brassica napus and B. nigra were combined via protoplast fusion into the novel hybrid Brassica naponigra. The heterokaryons were identified by fluorescent markers and selected by flow sorting. Thirty hybrid plants were confirmed by isozyme analysis to contain both B. nigra and B. napus chromosomes; of these, 20 plants had the sum of the parental chromosome numbers. A non-random segregation of the chloroplasts was found in the hybrids. Of 14 hybrid plants investigated, all had the B. napus type of chloroplast. The resistance to Phoma lingam found in the B. nigra cultivar used in the fusion experiments was expressed in 26 of the hybrid plants. The hybrids obtained in this study contain all of the three Brassica genomes (A, B and C) and have thus created unique possibilities for genetic exchanges between the genomes. Since most of the plants were fertile as well as resistant to P. lingam, they have been incorporated into conventional rapeseed breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261238
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  • 9
    Electronic Resource
    Electronic Resource
    Introgression in Brassica napus for adaptation to the growing conditions in Bangladesh (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 721-728 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Introgression ; Adaptation to short day ; Photo-insensitivity ; Oligogenic control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Among the oleiferous Brassicas, B. napus has the highest seed and oil productivity. As it is a species adapted to the temperate regions, its spring type is either unable to flower or flowers too late in the short — day winter (rabi) season of the subtropics. B. napus (genome AACC) is an amphidiploid between B. campestris (AA) and B. oleracea (CC), and shares one genome with the other allotetraploids B. juncea (AABB) and B. carinata (BBCC). While B. napus lacks ecotypes adapted to the subtropics, the other four species are well represented in this climatic zone. Reciprocal crosses with or without one direct backcross to B. napus have been carried out with the intention of transfering short-day adaptability. The aim was to introgress the A genome of carefully selected early representatives of B. campestris and B. juncea with the corresponding genome in B. napus, and similary the C genome from B. oleracea and B. carinata with the analogous genome in B. napus. B. campestris, B. juncea and the clearly later species, B. oleracea var ‘alboglabra’ and B. carinata, seem to be almost equally effective in introgressing the appropriate earliness necessary for growth in Bangladesh. One backcross sligthly delayed segregation of early types. Convergent crosses did not result in the transgression of earliness, which was unexpected since the inheritance of flowering and maturity indicated a polygenic regulation. This result is partly explained by assuming dominant oligogenic control of the photoperiodic response. Introgression of earliness with the C genome doesn't seem to be necessarily related with the earliness of the donor species. Intergenomic interactions may be important. Interesting new lines were selected with high yield. Thus there is a good probability that Bangladesh will have a new oil crop. As these lines were observed to be early in Sweden as well, they could potentially push rapseed cultivation further north in temperate regions where the growing period is limited by short summers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261250
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  • 10
    Electronic Resource
    Electronic Resource
    Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 194-200 
    Details
    ISSN: 1432-2242
    Keywords: Agrobacterium-transformation ; Asymmetric protoplast fusion ; Brassica napus ; Brassica nigra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary With the idea to develop a selection system for asymmetric somatic hybrids between oilseed rape (Brassica napus) and black mustard (B. nigra), the marker gene hygromycin resistance was introduced in this last species by protoplast transformation with the disarmed Agrobacterium tumefaciens strain C58 pGV 3850 HPT. The B. nigra lines used for transformation had been previously selected for resistance to two important rape pathogens (Phoma lingam, Plasmodiophora brassicae). Asymmetric somatic hybrids were obtained through fusion of X-ray irradiated (mitotically inactivated) B. nigra protoplasts from transformed lines as donor with intact protoplasts of B. napus, using the hygromycin resistance as selection marker for fusion products. The somatic hybrids hitherto obtained expressed both hygromycin phosphotransferase and nopaline synthase genes. Previous experience with other plant species had demonstrated that besides the T-DNA, other genes of the donor genome can be co-transferred. In this way, the produced hybrids constitute a valuable material for studying the possibility to transfer agronomically relevant characters — in our case, diseases resistances — through asymmetric protoplast fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288799
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