Summary
The response of oilseed rape cultivars to infection with Agrobacterium tumefaciens and A. rhizogenes and the possibility of regenerating genetically transformed oilseed rape plants were examined. The frequency at which Agrobacterium induced galls or hairy-roots on in vitro cultured plants ranged from 10% to 70%, depending on the cultivar. From galls induced by the tumorigenic strain T37, known to be strongly shoot inducing on tobacco, roots developed frequently. Occasionally, shoots formed and some of these produced tumour cell specific nopaline. Attempts to grow the transformed shoots into plants have so far been unsuccessful. Whole plants transformed with Ri-T-DNA, however, were regenerated. These had crinkled leaves and abundant, frequently branching roots that showed reduced geotropism, similar to previously isolated Ri T-DNA transformed tobacco and potato plants. The transformed oilseed rape plants flowered, but failed to form seeds.
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References
Ackermann C (1977) Pflanzen aus Agrobacterium rhizogenes tumoren an Nicotiana tabacum. Plant Sci Lett 8:23–30
Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12:8711–8721
Braun AC (1959) A demonstration of the recovery of the crown gall tumor cell with the use of complex tumors of single-cell origin. Proc Natl Acad Sci USA 45:932–938
Chilton M-D, Drummond MH, Merlo KJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11:263–271
Chilton M-D, Saiki RK, Yadav N, Gordon MP, Quetier F (1980) T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells. Proc Natl Acad Sci USA 77:4060–4064
Chilton MD, Tepfer DA, Petit A, David CC, Delbert F, Tempe J (1982) Agrobacterium rhizogenes inserts T-DNA into plant roots. Nature 295:432–434
DeBlock M, Herrera-Estrella L, Van Montagu M, Schell J, Zambryski P (1984) Expression of foreign genes in regenerated plants and in their progeny. EMBO J 3:1681–1689
Collier GF, Tibbits TW (1982) Tipburn of lettuce. Hortic Rev 4:49–65
Garfinkel DJ, Simpson RB, Ream LW, White FF, Gordon MP, Nester EW (1981) Genetic analysis of crown gall: fine structure map of the T-DNA by site directed mutagenesis. Cell 27:143–154
Glimelius K (1984) High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae. Physiol Plant 61:38–44
Horsch R, Haley RT, Rogers SG, Sanders PR, Lloyd A, Hoffmann N (1984) Inheritance of functional genes in plants. Science 223:496–498
Huffman GA, White FF, Gordon MP, Nester EW (1984) Hairy-root inducing plasmid: physical map and homology to tumor inducing plasmids. J Bacteriol 157:269–276
Karp A, Nelson RS, Thomas E, Bright SWJ (1982) Chromosome variation in protoplast-derived potato plants. Theor Appl Genet 63:265–272
Kartha KK, Michayluk MR, Kao KN, Gamborg OL, Constabel F (1974) Callus formation and plant regeneration from mesophyll protoplasts of rape plants (Brassica napus L cv ‘Zephyr’). Plant Sci Lett 3:265–271
Kohlenbach HW, Wenzel G, Hoffmann F (1982) Regeneration of Brassica napus plantlets in cultures from isolated protoplasts of haploid stem embryos as compared with leaf protoplasts. Z Pflanzenphysiol 105:131–142
Leemans J, Deblaere R, Willmitzer L, de Greve H, Hernalsteens JP, Van Montagu M, Schell J (1982) Genetic identification of functions of Ti T-DNA transcripts in octopine grown galls. EMBO J 1:147–152
Lu DY, Pental D, Cocking EC (1982) Plant regeneration from seedling cotyledon protoplasts. Z Pflanzenphysiol 107: 59–63
Marriotti D, Davey MR, Draper J, Freeman JP, Cocking EC (1984) Crown gall tumorigenesis in the forage legume Medicago sativa. Plant Cell Physiol 25:474–482
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Norton RA, Towers GHN (1984) Transmission of nopaline crown gall tumor markers through meiosis in regenerated whole plants of Bidens alba. Can J Bot 62:408–413
Ooms G, Hooykaas PJJ, Moolenaar G, Schilperoort RA (1981) Crown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids: analysis of T-DNA functions. Gene 14:33–50
Ooms G, Karp A, Roberts J (1983) From tumour to tuber: tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv ‘Maris Bard’). Theor Appl Genet 66:169–172
Ooms G, Karp A, Burrell MM, Twell D, Roberts J (1985) Genetic modification of potato development using Ri T-DNA. Theor Appl Genet 70:440–446
Otten LABM, Schilperoort RA (1978) A rapid microscale method for the detection of lysopine and nopaline dehydrogenase activities. Biochim Biophys Acta 527:497–500
Pavingerova D, Ondrej M, Matousek J (1983) Analysis of progeny of Arabidopsis thaliana plants regenerated from crown gall tumors. Z Pflanzenphysiol 112:427–433
Petit A, David C, Dahl GA, Ellis JG, Guyon P, Casse-Delbart F, Tempe J (1983) Further extension of the opine concept: plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol Gen Genet 190:204–214
Riker AJ, Banfield WM, Wright WH, Keitt EW, Sagan HE (1930) Studies on infectious hairy-root of nursery apple trees. J Agric Res 41:507–540
Smith EF, Townsend CO (1907) A plant-tumor of bacterial origin. Science 25:671–673
Tepfer D (1984) Transformation of several species of higher-plants by Agrobacterium rhizogenes — sexual transmission of the transformed genotype and phenotype. Cell 37: 9159–9167
Thomas E, Hoffmann F, Potrykus J, Wenzel G (1976) Protoplast regeneration and stem embryogenesis of haploid androgenetic rape. Mol Gen Genet 145:245–247
Thomashow MF, Nutter R, Montoya AL, Gordon MP, Nester EW (1980) Integration and organisation of Ti plasmid sequences in crown gall tumors. Cell 19:729–739
Turgeon R, Wood HN, Braun AC (1976) Studies on the recovery of crown gall tumor cells. Proc Natl Acad Sci USA 73:3562–3564
White FF, Ghidossi G, Gordon MP, Nester EW (1982) Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome. Proc Natl Acad Sci USA 79:3193–3197
Willmitzer L, De Beuckeleer M, Lemmers M, Van Montagu M, Schell J (1980) The Ti-plasmid derived T-DNA is present in the nucleus and absent from plastids of plant crown gall cells. Nature 287:359–361
Wullems GJ, Molendijk L, Ooms G, Schilperoort RA (1981) Retention of tumor markers in F1 progeny plants from in vitro induced octopine and nopaline tumor tissues. Cell 27: 719–727
Xu ZH, Davey MR, Cocking EC (1982) Plant regeneration from root protoplasts of Brassica. Plant Sci Lett 24:117–121
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Communicated by R. Riley
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Ooms, G., Bains, A., Burrell, M. et al. Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium . Theoret. Appl. Genetics 71, 325–329 (1985). https://doi.org/10.1007/BF00252075
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DOI: https://doi.org/10.1007/BF00252075