ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (54)
  • Articles: DFG German National Licenses  (54)
  • Rat  (39)
  • Hordeum  (15)
  • Springer  (54)
  • American Chemical Society
  • Blackwell Publishing
  • 1980-1984  (54)
  • 1925-1929
  • 1980  (54)
  • 1925
  • Biology  (54)
Collection
  • Articles  (54)
Source
  • Articles: DFG German National Licenses  (54)
Keywords
Publisher
  • Springer  (54)
  • American Chemical Society
  • Blackwell Publishing
Years
  • 1980-1984  (54)
  • 1925-1929
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    Fine structure of fetal rat calvarium; provisional identification of preosteoclasts (1980)
    Springer
    Calcified tissue international 31 (1980), S. 21-28 
    Details
    ISSN: 1432-0827
    Keywords: Rat ; Calvarium ; Electron microscopy ; Preosteoclasts ; Osteoclasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary This is a study of the fine structure of cells of the 20-day fetal rat calvarium. Special attention is given to identifying and characterizing preosteoclasts. These cells are relatively common and located largely, but not exclusively, at the endocranial bone surface. The preosteoclasts are characterized by abundant mitochondria, an incomplete perinuclear Golgi apparatus, and variable-shaped dense granules. The dense granules are unique in appearance in that they contain an internal dense matrix surrounded by a clear halo. Most granules are circular in shape but some are elongate or tubular in form. Granules with identical appearance are observed in osteoclasts. The preosteoclasts are mononucleate, or occasionally binucleate. It is suggested that because preosteoclasts are morphologically distinctive and relatively abundant, it should be feasible to separate these cells from a heterogeneous cell isolate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02407163
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    An automatic microspectrophotometric scanning method for the measurement of bone formation rates in vivo (1980)
    Springer
    Calcified tissue international 32 (1980), S. 63-68 
    Details
    ISSN: 1432-0827
    Keywords: Rat ; Bone formation ; Fluorochrome ; Microphotometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary A method for quantitative studies of the formation rate of bone has been developed. After vital staining with calcein, the fluorescence of a bone section was measured with a microphotometer controlled by a mini computer. After staining the bone structure with alizarin red S in a second step, the section was measured in transmitted light. The two data sets were combined and the shortest distances between the bone surface and the fluorescence lines were computed. With this information the distance distribution and the bone area between the label and the surface could be calculated in two different ways: with the single labeling and the continuous labeling techniques. The advantages and disadvantages of the two methods are discussed and compared with those of other techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408522
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A quantitative histologic analysis of the growing long bone metaphysis (1980)
    Springer
    Calcified tissue international 32 (1980), S. 113-122 
    Details
    ISSN: 1432-0827
    Keywords: Rat ; Bone ; Metaphysis ; Quantitative ; Aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The purpose of this work was to analyze the proximal tibial metaphysis of the 170 g rat in a quantitative histologic fashion which would allow some relation to tissue age to be established. Stained 3 µm thick tissue sections were analyzed with the aid of a Merz grid on an eyepiece reticule and a light microscope. Tissue mass and cell distribution were studied in all areas. The rate of change in tissue mass during aging of the metaphysis was calculated. Two regions of the metaphysis were identified. One, corresponding to the primary spongiosa, less than 4.45 days of age, is a region of high turnover of hard tissue and high numbers of osteoblasts and osteoprogenitor cells. The other, corresponding to the secondary spongiosa, is a region of relatively low net tissue turnover and low numbers of osteoblasts and osteoprogenitor cells. Osteoclasts were found relatively more uniformly distributed through the metaphysis than were osteoblasts and osteoprogenitor cells. The rate of bone formation in the primary spongiosa is 50 times that found in the Haversian bone of the rib of 5-year-old humans and about 500 times that found at the cortical-endosteal surface of ribs of 5-year-old humans. It is argued that both cell distribution and tissue distribution in the metaphysis support the concept that osteoblasts and osteoclasts, rather than osteocytes, are responsible for the maturation of the metaphysis. The inhomogeneous distribution of both cells and tissue in the metaphysis has definite meaning for the interpretation of findings concerning the incorporation of radionuclides into the skeleton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408530
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Role of growth hormone in experimental phosphorus deprivation in the rat (1980)
    Springer
    Calcified tissue international 32 (1980), S. 105-112 
    Details
    ISSN: 1432-0827
    Keywords: Rat ; Hypophysectomy ; Dietary phosphorus deprivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The demands of growth are known to exacerbate the effect of phosphorus deprivation (PD). We examined whether changes associated with PD could be prevented in young rats in which growth and growth hormone (GH) were eliminated by hypophysectomy (HPX) and whether PD in normal intact rats (INT) was associated with increased secretion of GH. INT or thyroxine- and ACTH-replaced HPX rats were fed one of the three diets: 0.31% P (NP); 0.027% P (LP), and 0.31% P, pair-fed with LP-mates (NP-PF). The results indicate that HPX did not qualitatively alter several physiologic responses to PD: (a) serum and urinary phosphorus (P) decreased and urinary calcium (Ca) increased; (b) net intestinal Ca retention fell and duodenal sac uptake of45Ca rose; and (c) external P balance was restored and duodenal sac uptake of32P-phosphate increased. Only the hypercalcemia seen in INT, LP rats was prevented by HPX. In INT rats serum immunoassayable GH levels, measured in single samples, were not different between different dietary groups while pituitary bioassayable GH was reduced in both LP and NP-PF rats when compared to the NP rats. Thus, except for hypercalcemia, the physiologic responses associated with PD are not prevented by the elimination of growth and GH, and the development of these responses in INT rats was not associated with a consistent or specific alteration in GH secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408529
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Kinetics of the appearance of mRNA for light-harvesting chlorophyll a/b protein in polysomes of barley (1980)
    Springer
    Planta 148 (1980), S. 448-452 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyll protein ; Hordeum ; Photosynthesis (light harvesting) ; Poly(A)RNA ; Polysomes ; Thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polysomes from dark-grown and illuminated barley seedlings were translated in cell-free systems. The translation products reacting with the antibody against the light-harvesting chlorophyll a/b protein (LHCP) were analyzed by polyacrylamide gel electrophoresis. It was found that, in addition to the precursor protein of LHCP, a product was obtained that co-migrated with the mature protein. Furthermore, the results show that the light-induced proly(A)RNA for LHCP is integrated into the polysomal complex without delay, indicating that the integration of LHCP into the membrane is controlled at a higher level of gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395313
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    The effect of differential root and shoot temperature on the nitrate reductase activity, assayed in vivo and in vitro in roots ofHordeum vulgare (barley) (1980)
    Springer
    Planta 148 (1980), S. 455-461 
    Details
    ISSN: 1432-2048
    Keywords: Carbohydrates (in roots) ; Hordeum ; Malate ; Nitrate reductase activity ; Temperature (and nitrate reductase activity)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There was a large increase in nitrate reductase activity (NAR) assayed both in vivo and in vitro in roots of barley plants (cv. Midas_ grown with roots at 10°C and shoots at 20°C, compared with whole plants grown at 20°C. There were diurnal fluctuations in NRA in roots from both treatments, but they were much greater in roots grown at 20°C, where NRA fell to a very low value in the dark period. The diurnal fluctuations in the malate content of the roots were also related to the root growth temperature. Plants with roots grown at the lower temperature had a higher malate content, especially in the dark period where it was 20 times greater than in plants with roots at 20°C. At all times there was a three-fold increase in soluble carbohydrate in cooled roots and diurnal fluctuations were much less pronounced than those of malate. Growth at low temperatures increased the total flux of amino N into the xylem sap and increased the proportion of reduced N in the total N flux. At certain times of day both 10°C- and 20°C-grown roots responded to exogeneous malate by increasing the flux of amino acid into the xylem sap, although this effect was always more pronounced in 20°C-grown roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395315
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Mobilization of proline in the starchy endosperm of germinating barley grain (1980)
    Springer
    Planta 149 (1980), S. 149-154 
    Details
    ISSN: 1432-2048
    Keywords: Endosperm ; Germination (seeds) ; Hordeum ; Proline ; Reserve mobilization (seeds) ; Seed germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In germinating grains of barley, Hordeum vulgare L. cv. Himalaya, free proline accumulated in the starchy endosperm during the period of rapid mobilization of reserve proteins. When starchy endosperms were separated from germinating grains and homogenized in a dilute buffer of pH 5 (the pH of the starchy endosperm), the liberation of proline continued in these suspensions. The process was completely inhibited by diisopropylfluorophosphate, indicating that it was totally dependent on serine carboxy-peptidases. The carboxypeptidases present in the starchy endosperms of germinating grains were fractionated by chromatography on DEAE-cellulose. Four peaks were obtained, all with different activity spectra on the seven carbobenzoxydipeptides (Z-dipeptides) tested. Two of the peaks corresponded to previously known barley carboxypeptidases; these as well as a third peak hydrolyzed substrates of the types Z-X-Y and Z-X-Pro (X and Y denote any amino acid residue except proline). The fourth peak corresponded to a proline carboxypeptidase specific for substrates of the Z-Pro-X type. Apparently, in the hydrolysis of longer proline-containing peptides there must be sequential cooperation between the two carboxypeptidase types. The carboxypeptidases in extracts of starchy endosperms also liberated proline from the peptides Ala-Ala-Ala-Pro and Ala-Ala-Pro while Ala-Pro and Pro-Ala were not attacked. The dipeptides, however, were rapidly hydrolyzed around pH 7 by extracts prepared from the scutella of germinating grains. It is concluded that one part of the proline residues of the reserve proteins is liberated in situ in the starchy endosperm through the combined action of acid proteinases and carboxypeptidases, while another part is taken up in the form of small peptides by the scutellum, where proline is liberated by amino- and/or dipeptidases in some “neutral compartment”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380876
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Nature of the phytochrome effect on the binding of glycolate oxidase to peroxisomes in vitro (1980)
    Springer
    Planta 149 (1980), S. 252-256 
    Details
    ISSN: 1432-2048
    Keywords: Glycolate oxidase ; Hordeum ; Phytochrome ; Peroxisomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The attachment of glycolate oxidase to the peroxisomal fraction derived from etiolated barley leaves (Hordeum vulgare L. cr. Dvir) is affected by light. The effect of red irradiation is reversed by subsequent far-red irradiation, indicating the involvement of phytochrome. This phytochrome effect is assumed to be related to phytochrome binding. Indeed, prevention by filipin (1.2·10-6 mol g-1 f wt) or cholesterol of phytochrome binding to membranes abolishes the effect of light on the interaction between glycolate oxidase and the peroxisomal fraction. Glycolate oxidase binding is affected by addition of quasi-ionophores such as gramicidin and filipin at a concentration of 0.6·10-3 mol g-1 f wt. This fact indicates that peroxisome-glycolate oxidase interaction may be affected by membrane potential. Since both ion transport and membrane potential are known to be affected by phytochrome, it is proposed that phytochrome acts in the light-induced modulation of glycolate oxidase attachment as a quasi-ionophore.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384561
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    In vitro synthesis of barley storage proteins (1980)
    Springer
    Planta 149 (1980), S. 262-268 
    Details
    ISSN: 1432-2048
    Keywords: Hordeum ; Polyribosomes ; Protein synthesis ; RNA ; Seeds ; Storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Membrane-bound polysomes were isolated from developing endosperms of barley (Hordeum vulgare L.) and shown to support the synthesis of trichloroacetic acid-insoluble material by an in vitro wheat germ protein synthesis system. The mRNA associated with the polysomes was separated from the ribosomes by affinity chromatography on oligo-dT cellulose and was also shown to support in vitro protein synthesis. The poly-A+ RNA isolated contained material of between 0.55 and 2.55 kilobases in length with about 6% poly A. The products of in vitro protein synthesis resembled hordeins (the prolamin storage proteins of the barley endosperm) in that they were predominantly soluble in 55% propan-2-ol, contained a low proportion of lysine as compared with leucine and had similar, but not identical, electrophoretic properties. The differences in the electrophoretic behaviour between the products of poly-A+ RNA translation and authentic hordeins is suggested to be due to the presence of an extra (leader?) sequence on the former.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384563
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Differential expression of the genes for ribulose-1,5-bisphosphate carboxylase and light-harvesting chlorophyll a/b protein in the developing barley leaf (1980)
    Springer
    Planta 150 (1980), S. 41-45 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyll-protein ; Hordeum ; Leaf development ; Light-harvesting chlorophyll a/b protein ; mRNA ; Ribulose-1,5-bisphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression of genes in particular for light-harvesting chlorophyll a/b protein (LHCP) and ribulose-1,5-bisphosphate carboxylase (RuBPCase) has been studied in the developing barley leaf. This has been done by analysis of the occurrence of both proteins within the different regions (1 to 6, beginning from the base) of the primary 7-day-old leaf. It has been found that LHCP already appears in the base of the leaf, whereas RuBPCase is primarily expressed in the apical expanding part of the leaf. The distribution of the mRNAs for both proteins within this gradient is in accordance with that of the proteins themselves, indicating that gene expression is not regulated at the level of translation in both cases. The poly(A) mRNA for LHCP occurs mainly in the basic sections 2 and 3, whereas that for RuBPCase is found throughout the leaf but primarily in the apical sections of the leaf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385613
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    The effect of differential root and shoot temperature on the nitrate reductase activity, assayed in vivo and in vitro in roots ofHordeum vulgare (barley) (1980)
    Springer
    Planta 148 (1980), S. 455-461 
    Details
    ISSN: 1432-2048
    Keywords: Carbohydrates (in roots) ; Hordeum ; Malate ; Nitrate reductase activity ; Temperature (and nitrate reductase activity)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There was a large increase in nitrate reductase activity (NAR) assayed both in vivo and in vitro in roots of barley plants (cv. Midas_ grown with roots at 10°C and shoots at 20°C, compared with whole plants grown at 20°C. There were diurnal fluctuations in NRA in roots from both treatments, but they were much greater in roots grown at 20°C, where NRA fell to a very low value in the dark period. The diurnal fluctuations in the malate content of the roots were also related to the root growth temperature. Plants with roots grown at the lower temperature had a higher malate content, especially in the dark period where it was 20 times greater than in plants with roots at 20°C. At all times there was a three-fold increase in soluble carbohydrate in cooled roots and diurnal fluctuations were much less pronounced than those of malate. Growth at low temperatures increased the total flux of amino N into the xylem sap and increased the proportion of reduced N in the total N flux. At certain times of day both 10°C- and 20°C-grown roots responded to exogeneous malate by increasing the flux of amino acid into the xylem sap, although this effect was always more pronounced in 20°C-grown roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552660
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    The isolation of endoplasmic reticulum from barley aleurone layers (1980)
    Springer
    Planta 150 (1980), S. 58-69 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone ; α-Amylase ; Endoplasmic reticulum ; Hordeum ; Organelle isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Techniques for the isolation and purification of endoplasmic reticulum (ER) from aleurone layers of barley (Hordeum vulgare L.) were assessed. Neither differential centrifugation nor density gradient centrifugation of a homogenate separate the ER or other organelles of this tissue from the lipidcontaining spherosomes. Isopycnic sucrose gradient centrifugation of organelles first purified by molecular sieve chromatography on Sepharose 4B, however, results in separation of the organelles based on their differing buoyant densities. Manipulation of the magnesium concentration of the isolation media and density-gradient solutions affords isolation of ER at a density of 1.13–1.14 g cc-1 and 1.17–1.18 g cc-1. Electron microscopy shows that the membranes sedimenting at 1.13–1.14 g cc-1 are devoid of ribosomes and are characteristic of smooth ER, while those sedimenting at 1.17–1.18 g cc-1 are studded with ribosomes and have the features of rough ER. Endoplasmic reticulum isolated by isopycnic density gradient centrifugation can be further purified by rate-zonal centrifugation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385616
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Quantitative and qualitative changes in the endoplasmic reticulum of barley aleurone layers (1980)
    Springer
    Planta 150 (1980), S. 70-81 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone ; α-Amylase ; Endoplasmic reticulum ; Gibberellin ; Hordeum ; Organelle isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in the level of the endoplasmicreticulum (ER) marker enzyme cytochrome-c reductase (EC 1.6.2.1) were followed with time of imbibition of de-embryonated half-seeds of barley (Hordeum vulgare L.) and the subsequent incubation of their aleurone layers in gibberellic acid (GA3) and H2O. During imbibition there is an increase in the level of cytochrome-c-reductase activity and in the amount of 280-nm absorbance associated with this enzyme. When aleurone layers are incubated for a further 42 h in water, there is a doubling of the cytochrome-c-reductase activity. In GA3, the activity of cytochrome-c reductase reaches a maximum at 24 h of incubation and thereafter falls to below 70% of its level at the beginning of the incubation period. Changes in the cytochrome-c-reductase activity correlate with changes in the fine structure of the aleurone cell. The ER isolated in low Mg2+ from aleurone layers incubated in buffer for up to 18 h has buoyant density of 1.13–1.14 g cc-1 while that from layers incubated in GA3 for 7.5–18 h has a density of 1.11–1.12 g cc-1. The α-amylase (EC3.2.1.1) isolated with the organelle fraction by Sepharose gel filtration is associated with the ER on isopycnic and rate-zonal density gradients, and its activity can be enhanced by Triton X-100. The soluble α-amylase fraction from Separose-4B columns, on the other hand, is not Triton-activated but is acid-labile. Acid phosphatase (EC3.1.3.2) is distributed in at least three peaks on isopycnic gradients. In low Mg2+ the second peak of activity has a density of 1.12 g cc-1 in GA3-treated tissue and 1.13–1.14 g cc-1 in H2O-treated tissue. With high-Mg2+ buffers, this peak of phosphatase activity disappears. Acid-phosphatase activity is not enhanced by Triton X-100 nor is it acid-labile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385617
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    The presence of phytochrome in purified barley etioplasts and its in vitro regulation of biologically-active gibberellin levels in etioplasts (1980)
    Springer
    Planta 148 (1980), S. 312-318 
    Details
    ISSN: 1432-2048
    Keywords: Etioplast ; Gibberellin ; Hordeum ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Data are presented confirming that purified barley etioplasts contain, or have associated with them, consistently detectable amounts of photoreversible phytochrome. Etioplasts, separated from mitochondrial contamination by sucrose gradient centrifugation, respond in vitro to red light treatment by an increase in the level of extractable gibberellin-like substances. It is concluded that earlier reports of the substances. It is concluded that earlier reports of the phytochrome regulation of biologically-active gibberellin levels in crude plastid fractions represent responses of the etioplast alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388117
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Primer dependent and independent forms of soluble starch synthetase from developing barley endosperms (1980)
    Springer
    Planta 148 (1980), S. 412-416 
    Details
    ISSN: 1432-2048
    Keywords: α-Glucan ; Hordeum ; Starch synthetase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The activity of soluble starch synthetase (ADP-glucose: α-1,4-glucan α-4-glucosyltransferase) in the non-purified extract from 16 day-old Bomi barley endosperms (Hordeum vulgare L.) was low and the reaction was non-linear when plotted against protein concentration. Starch synthetase was purified by ammonium sulfate precipitation and DEAE-cellulose chromatography and separated into four fractions. In the absence of an added carbohydrate primer two of the four fractions catalized the synthesis of a methanol-precipitable α-glucan when high concentrations of sodium citrate and bovine serum albumim were added. The rate of α-glucan synthesis by the unprimed reaction was higher than for the primed reaction. The four enzyme fractions were active with ADP-Glc, but not with UDP-Glc, both in the primed and in the unprimed reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388131
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    Kinetics of the appearance of mRNA for light-harvesting chlorophyll a/b protein in polysomes of barley (1980)
    Springer
    Planta 148 (1980), S. 448-452 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyll protein ; Hordeum ; Photosynthesis (light harvesting) ; Poly(A)RNA ; Polysomes ; Thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polysomes from dark-grown and illuminated barley seedlings were translated in cell-free systems. The translation products reacting with the antibody against the light-harvesting chlorophyll a/b protein (LHCP) were analyzed by polyacrylamide gel electrophoresis. It was found that, in addition to the precursor protein of LHCP, a product was obtained that co-migrated with the mature protein. Furthermore, the results show that the light-induced proly(A)RNA for LHCP is integrated into the polysomal complex without delay, indicating that the integration of LHCP into the membrane is controlled at a higher level of gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552658
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Symplasmic and apoplasmic radial ion transport in plant roots (1980)
    Springer
    Planta 148 (1980), S. 130-137 
    Details
    ISSN: 1432-2048
    Keywords: Hordeum ; Ion localization ; Ion uptake ; Plasmolysis ; Symplast uptake (ions)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The kinetics and localization of Rb+ uptake by barley roots were studied as function of the water potential of the medium. At potentials causing plasmolysis, uptake is reduced and becomes restricted to the outer surface of the root. The conclusion is that, at least for Rb+ in the range of low external concentrations (〈1 mM), the mature root cortex has little if any primary absorption1 capacity. Therefore, the apoplasmic pathway is of little or no significance for radial transport, which occurs by primary absorption at the epidermis and — perhaps — hypodermis followed by symplasmis transport to and through the cortex. Young cortex cells possess a primary absorption capacity comparable to that of the epidermis, but this feature is (largely) lost during differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386413
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Translocation and metabolism of glycine betaine by barley plants in relation to water stress (1980)
    Springer
    Planta 150 (1980), S. 191-196 
    Details
    ISSN: 1432-2048
    Keywords: Betaine ; Hordeum ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The glycine betaine which accumulated in shoots of young barley plants (Hordeum vulgare L.) during an episode of water stress did not undergo net destruction upon relief of stress, but its distribution among plant organs changed. During stress, betaine accumulated primarily in mature leaves, whereas it was found mainly in young leaves after rewatering. Well-watered, stressed, and stressed-rewatered plants were supplied with [methyl-14C]betaine (8.5 nmol) via an abraded spot on the second leaf blade, and incubated for 3 d. In all three treatments the added 14C migrated more or less extensively from the second leaf blade, but was recovered quantitatively from various plant organs in the form of betaine; no labeled degradation products were found in any organ. When 0.5 μmol of [methyl-14C]betaine was applied via an abraded spot to the second leaf blades of well-watered, mildly-stressed, and stressed-rewatered plants, 14C was translocated out of the blades at velocities of about 0.2–0.3 cm/min which were similar to velocities found for applied [14C]sucrose. Heat-girdling of the sheath prevented export of [14C]betaine from the blade. When 0.5 μmol [3H]sucrose and 0.5 μmol [14C]betaine were suppled simultaneously to second leaf blades, the 3H/14C ratio in the sheath tissue was the same as that of the supplied mixture. After supplying tracer [14C]betaine aldehyde (the immediate precursor of betaine) to the second leaf blade, the 14C which was translocated into the sheath was in the form of betaine. Thus, betaine synthesized by mature leaves during stress behaves as an inert end product and upon rewatering is translocated to the expanding leaves, most probably via the phloem. Accordingly, it is suggested that the level of betaine in a barley plant might serve as a useful cumulative index of the water stress experienced during growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390825
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    The effect of light on the biosynthesis of the light-harvesting chlorophyll a/b protein (1980)
    Springer
    Planta 150 (1980), S. 426-430 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyll a/b protein, synthesis ; Hordeum ; Light-harvesting chlorophyll protein ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of light on the biosynthesis of the light-harvesting chlorophyll a/b protein (LHCP) is investigated in wild-type barley (Hordeum vulgare L.) and in the chlorophyll b-less mutant chlorina f2. In dark-grown plants a short red light pulse triggers the appearance of mRNA activity for the LHCP. While the accumulation of this mRNA is controlled by phytochrome (Apel (1979) Eur. J. Biochem. 97, 183–188), the red light treatment is not sufficient to induce the appearance of the LHCP within the membrane. Thus, at least one of the subsequent steps in the biosynthetic pathway leading to the assembly of the LHCP is controlled by light. The red light-induced mRNA is taken up into the polysomes during the subsequent dark period and is translated in vitro in a cell-free protein synthesizing system. However, an accumulation of the freshly synthesized polypeptide within the plant is not observed. The apparent instability of the polypeptide might be explained by the deficiency of chlorophyll in the red light-treated plants. In the chlorophyll b-less barley mutant chlorina f2 an accumulation of the freshly synthesized apoprotein of the LHCP can be observed in the light. Thus, chlorophyll a formation seems to be a light-dependent step which is required for the stabilization of the LHCP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390180
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    Vasopressin- and oxytocin-containing fibres in the pineal gland and subcommissural organ of the rat (1980)
    Springer
    Cell & tissue research 205 (1980), S. 11-17 
    Details
    ISSN: 1432-0878
    Keywords: Pineal organ ; Subcommissural organ ; Vasopressin ; Oxytocin ; Vasotocin ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vasopressin and oxytocin were specifically demonstrated in the rat brain using the unlabelled antibody-enzyme method and purification of the first antiserum. Vasopressin and oxytocin fibres extend via the subcommissural organ or habenular commissure into the pineal stalk and terminate in the anterior part of the pineal organ. In addition, immediately adjacent to the subsommissural organ many vasopressin-containing fibres run caudally toward the central grey. These results are discussed in relation to the proposed presence of vasotocin in the pineal gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234438
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    VIP (Vasoactive Intestinal Polypeptide)-immunoreactive neurons in the retina of the rat (1980)
    Springer
    Cell & tissue research 210 (1980), S. 167-170 
    Details
    ISSN: 1432-0878
    Keywords: Neuropeptides ; VIP-immunoreactive neurons ; Retina ; Amacrine cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunoreactive vasoactive intestinal polypeptide (VIP) was detected in a population of amacrine cells in the retina of the rat. Processes of these cells reach both the inner and outer half of the inner plexiform layer where they form sublayers. The VIP neurons are different from previously known amacrine cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232152
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    Simultaneous monoamine histofluorescence and neuropeptide immunocytochemistry (1980)
    Springer
    Cell & tissue research 210 (1980), S. 181-189 
    Details
    ISSN: 1432-0878
    Keywords: Catecholamines ; Neurophysin ; Simultaneous demonstration ; Functional interaction ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A method was developed that allows the analysis of neuropeptides and monoamines in a single tissue section by the application of the unlabeled antibody method for peptide staining to tissue sections freeze-dried for formaldehyde-induced monoamine histofluorescence. The hypothalamic magnocellular system of male albino rats served as a model for this study; neurons were stained with anti-neurophysin sera, which mark the vasopressin- and oxytocin-associated proteins. Neurophysin-containing perikarya appeared to be surrounded by catecholamine-containing varicosities. This phenomenon was seen to varying degrees within the supraoptic and paraventricular nuclei. The juxtaposition of varicosities and peptidergic neurons suggests an afferent fiber-target neuron relationship that might favor a functional interaction between monoamines and neuropeptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237608
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations associated with the initiation of follicular atresia (1980)
    Springer
    Cell & tissue research 211 (1980), S. 105-115 
    Details
    ISSN: 1432-0878
    Keywords: Rat ; Preovulatory follicle ; Ultrastructure ; Degeneration ; Atresia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To identify and describe ovarian follicles committed to undergo follicular degeneration (atresia), immature rats were primed with pregnant mare serum gonadotropin (PMSG). After PMSG treatment, preovulatory follicles develop but subsequently degenerate. Prior to the appearance of pyknotic nuclei (Stage I of atresia), degenerative changes were observed in focal areas of the granulosa cell layer. These changes include “blebbing” of the cytoplasm and alterations in the shape of the granulosa cells. The appearance of these degenerative changes coincides with a decrease in ovarian concentrations of estradiol and testosterone. Since estrogens and androgens maintain the follicle, the decline in estradiol and testosterone could be responsible for the further degenerative alterations that lead to complete deterioration of the preovulatory follicle. In Stage I atretic follicles, lysosome-derived autophagic vacuoles develop and macrophages invade both the thecal and granulosa cell layers. The combined actions of the autophagic vacuoles and macrophages could destroy both the granulosa-cell and thecal layers and thereby transform the preovulatory follicle into an ovarian cyst.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233727
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    Depletion of macrophages and disappearance of postcapillary high endothelial venules in lymph nodes deprived of afferent lymphatic vessels (1980)
    Springer
    Cell & tissue research 211 (1980), S. 375-389 
    Details
    ISSN: 1432-0878
    Keywords: Lymph node ; Macrophages ; Postcapillary high endothelial venules ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The afferent lymphatic vessels of rat popliteal lymph nodes were interrupted, and the histological alterations in the lymph nodes occurring 1 to 14 weeks after operation were studied. One week after operation the number of macrophages was considerably reduced and continued to decrease during the subsequent time periods studied. A 6 weeks most macrophages had disappeared. Simultaneously the immunological activity diminished and had completely disappeared 8 weeks after operation. Three weeks after operation the endothelial cells of the postcapillary high endothelial venules had flattened, and the number of immigrating lymphocytes was greatly reduced. Subsequently the lymph nodes became depleted of both macrophages and lymphocytes, leaving only the reticuloendothelial framework.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234394
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    A scanning electron microscope study of myoepithelial cells in exocrine glands (1980)
    Springer
    Cell & tissue research 209 (1980), S. 1-10 
    Details
    ISSN: 1432-0878
    Keywords: Myoepithelial cell ; Exocrine gland ; Scanning electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By removing connective tissue components with enzymatic digestion followed by HCl-hydrolysis, myoepithelial cells (MECs) of the terminal portion in a variety of exocrine glands of the rat were examined with the scanning electron microscope. The profile of MECs varied considerably from gland to gland; MECs in the lactating mammary gland have a few long cytoplasmic processes in close contact with those of adjacent cells forming a continuous network around the terminal portion. Those of the exorbital lacrimal gland are stellate with many thin radiating processes with tapered ends that terminate freely. MECs in the sublingual gland are characterized by a number of broad and extensive cellular processes. MECs in the submandibular gland are similar in appearance to those of the exorbital lacrimal gland, but with more extensive cellular processes that form a more or less continuous network with those of the adjacent cells. No MECs were observed on the terminal portion of the parotid gland where the cells appear to be lodged on the intercalated duct. The relative surface area covered by MECs per terminal portion was also found to vary significantly, being 24% in the lactating mammary, 17% in the exorbital lacrimal, 48% in the sublingual, and 25% in the submandibular glands. The findings are discussed in relation to the physical properties of secretions in different glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219918
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    Nongranular vasopressin synthesis and transport in early stages of rehydration (1980)
    Springer
    Cell & tissue research 207 (1980), S. 89-107 
    Details
    ISSN: 1432-0878
    Keywords: Rehydration ; Nongranular vasopressin ; Intercellular clefts ; Axoplasmic reticulum ; Ultrastructural immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The vasopressin system of the rat was examined in the course of the first 12 h of rehydration after prolonged thirst at light and electron microscopic levels and by use of the peroxidase anti-peroxidase (PAP) method. Light microscopically, the median eminence was the only part of the system that not only displayed distinct differences between animals of different rehydration times but also showed a characteristic pattern of immunohistochemical reactivity in its rostro-caudal distribution. Ultrastructurally, in the perikarya a maximal labeling of the rough endoplasmic reticulum was observed after 2 h of rehydration, whereas an extensive labeling of the enlarged Golgi zones was attained after 4 h of resupplying water. A labeling of the intercellular clefts in the basal glial labyrinth of the supraoptic nucleus (and to a lesser degree in the subependymal neuropil adjacent to the paraventricular nucleus) was increased 30 min after the onset of drinking, as compared with water-deprived animals; it decreased slightly after 12 h of rehydration. The filling of the swollen fibers by increasing amounts of labeled axoplasmic reticulum, evident in the nuclear areas already after 30 min of water supply, begins in the median eminence after 2 h of rehydration and is fully developed after 4 and 8 h. Corresponding results hold true for the neural lobe but are somewhat delayed in comparison to the findings in the median eminence. The discussion considers (i) synthesis and transport of nongranular vasopressin within the axoplasmic reticulum, and (ii) release not only from the neural lobe but also from the nuclear areas and from the fibers of the median eminence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239332
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    Functional reinnervation of the neostriatum in the adult rat by use of intraparenchymal grafting of dissociated cell suspensions from the substantia nigra (1980)
    Springer
    Cell & tissue research 212 (1980), S. 39-45 
    Details
    ISSN: 1432-0878
    Keywords: Neuronal grafting ; Dopamine ; Nigrostriatal system ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dissociated cell suspensions were prepared from the substantia nigra of 15–17 day-old rat embryos and grafted via an intraparenchymal injection into the depth of the neostriatum of adult recipient rats. The survival and fibre outgrowth of the dopamine-containing neurones in the implants were studied by fluorescence histochemistry, and the functional capacity of the grafts was monitored by repeated testing of the amphetamine-induced turning behaviour of the implanted rats. Before transplantation the target neostriatum of the recipient rats was denervated of its normal dopaminergic innervation by an injection of 6-hydroxydopamine into the ipsilateral nigrostriatal dopamine pathway. The completeness of the denervation was ascertained by measurement of the intensity of the amphetamine-induced turning response. After injection of the dissociated cells large numbers of dopamine-containing neurones were found in clusters at the site of injection as well as scattered in the apparently intact neostriatal tissue up to a distance of about 0.5 mm from the site of injection. Extensive dopamine-containing fibre networks had developed around the implant. These newly formed fibres, which were most abundant around the cell clusters at the injection site, extended in a loose network into large areas of the initially denervated caudate-putamen. In all animals with surviving dopamine neurones the amphetamine-induced turning response was reduced, and in the most extensively reinnervated cases even reversed, within 3–5 weeks after transplantation. This strongly suggests that the implanted dopamine neurones are capable of restoring dopaminergic neurotransmission in the denervated neostriatum, probably via reinnervation of the denervated neostriatal tissue. The use of dissociated brain tissue preparations thus permits reliable intraparenchymal grafting of neurones to plausibly any desired site within the central nervous system, and should open entirely new possibilities for investigation of neuronal growth dynamics and functional reconstruction of damaged brain circuits, perhaps even in brains of larger mammals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234031
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    A peculiar fibrillar pattern in the myocardial cells of trabeculae carneae in the right ventricle of the rat, mouse, and rabbit (1980)
    Springer
    Cell & tissue research 208 (1980), S. 165-168 
    Details
    ISSN: 1432-0878
    Keywords: Cardiac muscle cells ; Trabeculae carneae ; Fibrillar pattern ; Mouse ; Rat ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cardiac muscle fibers in the trabeculae carneae of mice, rats, and rabbits show a special arrangement of densely interwoven myofibrils. They cross at various angles; however, a preferred orientation of the fibrils cannot be discerned. It is suggested that due to this arrangement the myocytes of trabeculae are not able to contract to the same extent as ventricular myocytes, but thereby gain a high rigidity during contraction. Hence, they may play a principal role as “guiding ridges” for the flow of blood, thereby improving hemodynamics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234181
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    The influence of high pressure freezing on mammalian nerve tissue (1980)
    Springer
    Cell & tissue research 209 (1980), S. 201-216 
    Details
    ISSN: 1432-0878
    Keywords: High pressure (2,100 bars) freezing ; Freeze-etching ; Nerve tissue ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vitrification of biological specimens in liquid nitrogen can be achieved under high pressure (2,100 bars). This procedure obviates the use of aldehyde fixation and cryoprotection (glycerol). The present work demonstrates its applicability to the freeze-etching of mammalian brain tissue. Freeze-fracture replicas from rat cerebellar cortex and subfornical organ prepared by this method are compared to conventionally processed material using aldehyde fixation, glycerination and freezing with Freon. The formation of large ice crystals is prevented in tissue blocks up to 0.5 mm thick; deep etching is markedly enhanced. Cytoplasmic microstructures such as mitochondrial cristae, microtubules and microfilaments, are readily observable against a finely granulated cytosol matrix. An additional advantage is the combined application with freeze-substitution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237626
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    The structure of cleft material in spine synapses of rat cerebral and cerebellar cortices (1980)
    Springer
    Cell & tissue research 206 (1980), S. 477-486 
    Details
    ISSN: 1432-0878
    Keywords: Synapse ; Synaptic cleft ; Aldehyde ; PTA technique ; Densitometry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptic clefts of rat cerebral and cerebellar axodendritic spine synapses were studied after aldehyde-perfusion and subsequent immersion into osmic acid or after processing by the aldehyde-PTA technique. The threedimensional examination of aldehyde-perfused, osmic acid postfixed synapses revealed a double-layered intracleft lamina comparable in dimensions and position to the cleft density of non-osmicated, PTA-stained synapses. The relationship of this lamina to perisynaptic astroglial processes was pointed out. Densitometric analysis of the cleft area suggested the identity of the intracleft lamina of osmicated synapses with the cleft density of non-osmicated, PTA-stained synapses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237976
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    Studies on the juxtaglomerular apparatus VI. Sympathetic innervation, catecholamines and the renin-angiotensin-system in rats and tree-shrews (Tupaia belangeri) (1980)
    Springer
    Cell & tissue research 212 (1980), S. 375-382 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Sympathetic innervation ; Renin-angiotensin system ; Electron microscopy ; Fluorescence microscopy ; Tupaia belangeri ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary It has previously been reported that the primitive primate Tupaia belangeri develops a renal failure when exposed to psychosocial stress. In order to learn if this high susceptibility to stress of the Tupaia kidney can be correlated with morphological and functional parameters of the Juxtaglomerular apparatus (JGA) and the renin-angiotensin system, comparative experiments were performed on Tupaia and rat. Our results reveal an outstandingly high potency of the JGA and the renin-angiotensin system in Tupaia as evident from the following findings: The Tupaia JGA contains a great number of epithelioid cells abounding in renin granules (electron microscopy). The renin content of the Tupaia kidney is considerably higher than in the rat (radio-immunoassay). The sympathetic innervation of the kidney and especially of the JGA is abundant in Tupaia (fluorescence and electron microscopy). Catecholamine contents of the kidney and other organs are significantly higher in Tupaia than in rats (spectrophotofluorometry). Our results support the previously developed concept of a potent intrarenal neuroendocrine interaction at the JGA level favouring, under certain conditions of social stress, the development of acute renal failure in Tupaia belangeri.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236504
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations in the initial segments and in the recurrent collateral terminals of Purkinje cells following axotomy (1980)
    Springer
    Cell & tissue research 212 (1980), S. 415-427 
    Details
    ISSN: 1432-0878
    Keywords: Cerebellum ; Purkinje axon ; Ultrastructure ; Axotomy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Transection of Purkinje cell axons in adult male rats made 1.5 mm or further from the cell body does not lead to the death of the neuron and results in compensatory structural alterations of the surviving axonal portions of the nerve cell. Near to, and at the emergence of recurrent collaterals of Purkinje cell axons, huge varicosities filled with filaments, granular material, lysosomes and mitochondria develop. Terminals of recurrent axon collaterals also exhibit different degrees of structural changes. Most striking of the morphological alterations is the regular presence of nematosomes in the hypertrophic axonal branches, especially in synaptic terminals. Since nematosomes were shown to contain RNA in other types of neurons, their presence in recurrent collaterals may indicate an enhanced synthetic activity in Purkinje axonal processes and endings after axotomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236507
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Interlamellar tight junctions of central myelin (1980)
    Springer
    Cell & tissue research 213 (1980), S. 81-94 
    Details
    ISSN: 1432-0878
    Keywords: Interlamellar tight junctions ; Central myelin ; Myelogenesis ; Optic nerve ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The process of myelination in the central nervous system (CNS) of the rat (optic nerve) was studied with the freeze-fracturing technique and ultrathin sectioning to obtain information on the developmental mechanisms of interlamellar tight junctions. Using a tilting cartridge for analysis of thin sections, it could be demonstrated that during the initial phase of wrapping a tight junction formation develops between the joining tips of the oligodendrocytic process. In tannic acid-stained samples these junctions appear as typical quintuple-layered membrane fusions, while in potassium permanganate-stained material membrane thickenings between the apposing glial tips are prevalent. The latter configuration represents the characteristic feature of the so-called radial component of central myelin. Using the freeze-fracturing technique, a biphasic mode of the myelinic tight junction assembly was detected. It is suggested that tight junctions represent a prerequisite of the myelination process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236922
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    An intermittent somatostatin-immunoreactivity in the cortex and basal ganglia of the rat (1980)
    Springer
    Cell & tissue research 205 (1980), S. 327-331 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin ; Cortical cells and fibers ; Immunohistochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using light microscopic immunohistochemistry, somatostatinpositive structures were observed in the cortex of the rat. These structures, including cells and fibers, are widely distributed in all cortical laminae and are also found in the basal ganglia. The positive results were obtained exclusively in two groups of animals sacrificed during two different months of two subsequent years. The reason for this variability in the immunocytochemical stainability of cortical structures remains enigmatic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234691
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 35
    Electronic Resource
    Electronic Resource
    Architecture of normal pancreas as revealed by retrograde injection (1980)
    Springer
    Cell & tissue research 205 (1980), S. 445-451 
    Details
    ISSN: 1432-0878
    Keywords: Exocrine pancreas ; Rat ; Architecture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The architecture of the pancreas was revealed by retrograde injection of the pancreatic ductal system of normal rats with a silicone rubber compound, and subsequent study of the preparation by light microscopy and scanning electron microscopy. The injected material became associated with both ducts and “acinar” areas. Examination of these specimens suggests that the arrangement of the exocrine pancreas is that of a complexly curving and branching system of tubules which anastomose and end blindly. This architecture, which is not that of a true acinar gland, provides a rational basis for the understanding of the simple dedifferentiative changes that accompany pancreatic carcinogenesis, and which have been generally interpreted as representing ductular proliferation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232285
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 36
    Electronic Resource
    Electronic Resource
    Electron microscopic immunocytochemical investigation on the postnatal development of the vasopressin system in the rat (1980)
    Springer
    Cell & tissue research 205 (1980), S. 453-471 
    Details
    ISSN: 1432-0878
    Keywords: Vasopressin system ; Postnatal development ; Ultrastructural immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present ultrastructural results indicate that, in the rat, the vasopressin-synthesizing perikarya of the supraoptic nucleus (NSO) attain a certain degree of maturity earlier than those of the paraventricular nucleus (NPV). In the neonate rat, the stainability of the nuclear areas is very weak; in the perikarya of the NSO a few labeled granules can be found, whereas the perikarya of the NPV often display only a labeled Golgi area, the cytoplasm being devoid of granules. At the end of the first (NSO) and the second (NPV) postnatal weeks, the filling of the neurosecretory granules with vasopressin is inhomogeneous with irregular spots of reaction product distributed on the granules. This feature is less obvious during the following week and has nearly disappeared after the third and fourth postnatal weeks. Already in the neonate two types of vasopressin-positive fibers are observed in the median eminence, characterized by the different diameters of their granules and by their typical location in the internal and the external pericapillary contact zone. Especially in one and two week-old animals, in the internal zone of the median eminence and, to a lesser degree in the neural lobe, the immunocytochemical reaction product is deposited on an axonal tubular network. Judging from the presence of very few vasopressin-negative fibers in the neural lobe of the neonate, the development of the oxytocin system appears to be delayed. A characteristic relationship between pituicytes and the neurosecretory fibers can be observed during the first two postnatal weeks. After the third postnatal week the immunocytochemical features of the vasopressin system correspond approximately to that in adult rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232286
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 37
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of the cleft of the rat pituitary (1980)
    Springer
    Cell & tissue research 206 (1980), S. 139-143 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary cleft ; Scanning electron microscopy ; Cell surface changes ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Scanning electron microscopy of the lining of the pituitary cleft was carried out in normal, lactating, castrated, adrenalectomized, and cyproterone-treated adult rats. Four cell types could be differentiated in the posterior wall in control and experimental animals: (1) cells with a smooth surface, (2) cells with microvilli located at the cellular borders, (3) ciliated cells, and (4) cells with evenly distributed microvilli. The anterior wall showed mainly cells with few microvilli located at their margins, and clusters of ciliated cells. In normal, and more frequently in experimental animals, the anterior wall showed shriveled cells, and variously sized cavities. Colloid appeared either as a network of finely granular material or as compact bodies adhering to the epithelial surface. These observations suggest that a compact component of the colloid is derived at least in part from degraded cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233614
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 38
    Electronic Resource
    Electronic Resource
    Presence of AVT-, α-MSH-, LHRH- and somatostatin-like compounds in the rat pineal gland and their relationship with the UMO5R pineal fraction (1980)
    Springer
    Cell & tissue research 206 (1980), S. 341-353 
    Details
    ISSN: 1432-0878
    Keywords: AVT ; LHRH ; α-MSH ; Somatostatin ; Pineal Gland ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Resumé En utilisant des anticorps contre l'AVT, l'α-MSH, le LHRH et la somatostatine, des cellules épiphysaires du Rat ont été immunocytochimiquement colorées. Tous ces anticorps colorent les mêmes cellules. Ces cellules réagissent également quand un anticorps est utilisé contre la fraction épiphysaire UMO5R, fraction qui est douée de propriétés antigonadotropiques in vivo. Il a également été montré que le nombre des cellules immunoréactives était plus important dans la pinéale du jeune rat que dans celle de l'adulte. La comparaison des résultats obtenus avec différents anticorps et l'étude des propriétés de ces anticorps aprés absorption sur différents peptides ou sur différentes fractions épiphysaires, a permis de conclure que les réactions obtenues dans la pinéale du rat n'étaient que la conséquence d'une réaction croisée de ces anticorps avec une/des substance(s) inconnue(s) synthétisée(s) par la pinéale elle-même. La nature endocrine possible de cette substance qui serait chimiquement apparenté aux fractions épiphysaires Mouton UMO5R et Prot. 4, est discutée. Drs. B.L. Baker (Ann Arbor, Mich., USA), M.P. Dubois (Nouzilly, France), J. De Mey (Beerse, Belgium), J.D. Fernstrom (Cambridge, Mass., USA.), H. Goos (Utrecht, The Netherlands), B. Kerdelhué (Gif-sur-Yvette, France) and A.G.E. Pearse (London, U.K.) are also acknowledged for their gifts of various antibodies
    Notes: Summary Using antibodies against AVT, α-MSH, LHRH and somatostatin, immunoreactive cells were detected in the rat pineal gland. All of these antibodies stain the same cells, which also react immunocytochemically when an antibody against the UMO5R sheep pineal fraction, a fraction that presents antigonadotropic properties in vivo, is used. Relatively more immunoreactive cells are present in the pineals of young rats than in the pineals of adult animals. Comparison of the results obtained with different potent antibodies against each of the peptides, and a study of the staining properties of the antibodies in the pineal after solid phase adsorption to different peptides or to different sheep pineal fractions, led to the proposal that the immunoreactivity found in the rat pineal is not due to the presence of AVT, α-MSH, LHRH or somatostatin, but to a cross-reaction of each of these antibodies with (an) unidentified compound(s). This compound is synthetized in the pineal gland, as was demonstrated using cultured pineals. The UMO5R and the Prot. 4 fractions of the sheep pineal seem to be chemically related to this unknown compound, the possible endocrine nature of which is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237964
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 39
    Electronic Resource
    Electronic Resource
    Stimulation-depletion of serotonin and noradrenaline from vesicles of sympathetic nerves in the pineal gland of the rat (1980)
    Springer
    Cell & tissue research 207 (1980), S. 13-20 
    Details
    ISSN: 1432-0878
    Keywords: Sympathetic nerves ; Synaptic vesicles ; Noradrenaline ; Serotonin ; Pineal gland ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pineal gland of the rat receives a rich nervous supply originating from the superior cervical ganglia. These fibers contain serotonin in addition to their neurotransmitter, noradrenaline. Cytochemical studies at the ultrastructural level have shown that both amines are present in the cores of the granular vesicles that are characteristic of these nerves. It is presently shown that the bilateral electrical stimulation of the preganglionic fibers innervating the ganglia markedly reduces the number of small sites reacting cytochemically for both noradrenaline and serotonin, these sites corresponding to the cores of small granular vesicles, while the larger reactive sites (cores of large vesicles) remain unaltered. The vesicles are retained in nerve terminals after stimulation, as observed in conventionally processed tissues, although with altered sizes and shapes. Apart from these cytochemical and structural changes, nerve stimulation also reduces the endogenous noradrenaline content of the pineal gland. Thus, both noradrenaline and serotonin are released from their storage sites in pineal sympathetic nerves after electrical stimulation in vivo. This suggests the possibility that several substances with presumed transmitter or modulatory functions might be simultaneously released by nerve impulses from a given nerve terminal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239325
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 40
    Electronic Resource
    Electronic Resource
    Organ culture of the islets of Langerhans from young and senescent rats (1980)
    Springer
    Cell & tissue research 207 (1980), S. 429-448 
    Details
    ISSN: 1432-0878
    Keywords: Islet of Langerhans ; Aging ; Insulin-secreting cells ; Organ culture ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The B-cells of the endocrine pancreas constitute an adequate model for in vitro study of the aging process in highly differentiated cells. In the present study, collagenase-isolated islets of Langerhans from young and senescent rats were cultured up to 28 days. The response of the B-cells to the stimulatory conditions of the culture medium involved the nucleus, ribosomes, endoplasmic reticulum, Golgi apparatus, and secretory granules. Correlated data from light microscopy, electron microscopy, and insulin radioimmunoassay show that the differentiation and function of senescent B-cells are maintained in culture, as it has been proven for the B-cells of younger animals. On the other hand, signs of cytological deficiency not directly concerned with the specific function of B-cells were observed: abnormal mitochondria and lysosomes are more numerous in the senescent B-cells. The proliferative capacity of the B-cells of aged rats is reduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224618
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 41
    Electronic Resource
    Electronic Resource
    Dynamics of the transganglionic movement of horseradish peroxidase in primary sensory neurons (1980)
    Springer
    Cell & tissue research 207 (1980), S. 479-489 
    Details
    ISSN: 1432-0878
    Keywords: Horseradish peroxidase ; Primary afferent neurons ; Axonal transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dynamics of horseradish peroxidase (HRP) transport in primary sensory neurons were studied in rats by demonstration of the reaction product in spinal nerves, spinal ganglia, dorsal roots and in the spinal cord at different survival times after application of the enzyme to the transected sciatic nerve and to the spinal cord. Using tetramethylbenzidine as the chromogen according to Mesulam (1978), transganglionic transport of HRP was shown in both the disto-proximal direction after peripheral application, and proximo-distal direction after central application. Significant differences in staining intensity between the central and peripheral processes of primary sensory neurons were found after all survival times used in this study. After peripheral application the number of labeled axons and the staining intensity were higher in spinal nerves than in dorsal roots; an inverse situation occurred after central application. These differences as well as the time sequences in staining of different parts of primary sensory neurons suggest that HRP applied to a peripheral nerve and to the spinal cord, respectively, enters the perikarya of spinal ganglion cells in any case before continuing its movement in a cellulifugal direction. Lysosomal degradation of the major portion of the applied HRP is supposed. However, in the post-perikaryal portion of a considerable number of neurons HRP-transport still occurs to a varying extent, thus resulting in labeling of nerve endings. In some neurons a post-perikaryal transport could not be detected light microscopically. The transport rates differ: the calculated transport rate of disto-proximal, cellulipetal movement in the fastest transporting neurons was 7.5 mm/h, that of the disto-proximal cellulifugal movement 2.5 to 3 mm/h.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224621
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 42
    Electronic Resource
    Electronic Resource
    Changes of the caecal villi during postnatal development in rats (1980)
    Springer
    Cell & tissue research 208 (1980), S. 253-259 
    Details
    ISSN: 1432-0878
    Keywords: Villus ; Caecum ; Postnatal development ; Rat ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The villi of the caecal mucosa in postnatal rats were studied using both scanning electron and light microscopy. On the day of birth, numerous villi of various sizes and shapes were present on the caecal mucosa. After the 5th day, the villi decreased very rapidly in length and in number. A strong constriction was observed at the basal region of the caecal villi. During postnatal days 5 ∼ 9 the villi probably separated and disappeared from the caecal mucosa. No villi were observed in rats that were over 10 days of age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234875
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 43
    Electronic Resource
    Electronic Resource
    Hypothalamo-hypophysial function following the lesion of tanycytes in the median eminence of the rat (1980)
    Springer
    Cell & tissue research 209 (1980), S. 225-238 
    Details
    ISSN: 1432-0878
    Keywords: Tanycytes ; Median eminence ; Electron microscopy ; Rat ; Serum LH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The transport of hormones from the cerebrospinal fluid to the adenohypophysis by the tanycytes of the median eminence was examined in male rats. Electron microscopy revealed that all ependymal cells including the tanycytes disappear or degenerate in rats subjected to electric cauterization of the ependymal layer lining the third ventricle. However, the granular axons in the palisade layer of the median eminence remain intact. In rats subjected to electric lesion, no significant change was found in either the serum-LH level or in the weight of the adenohypophysis, testes, adrenal and thyroid glands. It is concluded that the tanycytes do not participate in the hypothalamic regulation of hypophysial function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237628
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 44
    Electronic Resource
    Electronic Resource
    Immunohistochemical study of the LHRH-synthesizing neuron system of aged female rats (1980)
    Springer
    Cell & tissue research 209 (1980), S. 499-503 
    Details
    ISSN: 1432-0878
    Keywords: LHRH perikarya ; Aging ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The LHRH-synthesizing neuron system was studied in young proestrous and old female rats, and in aged ovariectomized or reserpine-treated females. The medial preoptic area and septal region of old animals contains more LHRH positive perikarya compared to that of young proestrous rats. Reserpine treatment moderately increases the number of immunostainable LHRH cells, while ovariectomy is ineffective in this respect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234761
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 45
    Electronic Resource
    Electronic Resource
    Luliberin and somatostatin fiber-terminals in the subfornical organ of the rat (1980)
    Springer
    Cell & tissue research 210 (1980), S. 33-45 
    Details
    ISSN: 1432-0878
    Keywords: Luliberin (LRF)-terminals ; Somatostatin-terminals ; Subfornical organ ; Neurohemal regions ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the aid of light- and electron- microscopic immunocytochemistry, somatostatin- and luliberin (LRF)-positive fibers can be demonstrated in the rat subfornical organ (SFO). Each of the neurohormones has a specific location: LRF in the lateral parts of the organ, and somatostatin in the center of the posterior zone. Common to both neurohormone-containing fibers is the pattern in which they reach the organ as well as the fact that their terminals are located in the perivascular spaces of fenestrated vessels, i.e., within the limited neurohemal regions of the organ. Since injection of India ink of different colors demonstrates that the capillary bed of the SFO is connected with the central capillaries of the choroid plexus, the question arises as to whether the neurohormones released in the area of the SFO influence the choroid plexus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232139
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 46
    Electronic Resource
    Electronic Resource
    Morphological investigation of the deep pineal of the rat (1980)
    Springer
    Cell & tissue research 210 (1980), S. 283-294 
    Details
    ISSN: 1432-0878
    Keywords: Deep pineal ; Pineal complex ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The results presented here reveal that in adult Sprague-Dawley and Wistar rats the pineal gland represents a complex rather than a single organ. Regularly one can distinguish (i) pineal tissue in the intercommissural region as a deep pineal, (ii) a superficial pineal, which represents the major part of the pineal complex, and (iii) nearly always a parenchymal stalk of variable length. The volume of the deep pineal with the adjacent parenchymal stalk exhibits great interindividual variation. It amounts to 127±39×105 μm3 (mean ±standard deviation). The histological appearance of the deep and superficial pineal tissue is fairly similar. The intrinsic cells of the deep and superficial pineal differ in nuances only. Karyometry reveals that the nuclear volumes of the intrinsic cells of the deep pineal are very variable ranging from 90–450 μm3, with a mean value of 207 μm3. The changes over a period of 24 h reach statistical significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237616
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 47
    Electronic Resource
    Electronic Resource
    Investigations on the turnover of adrenocortical mitochondria (1980)
    Springer
    Cell & tissue research 210 (1980), S. 333-337 
    Details
    ISSN: 1432-0878
    Keywords: Zona glomerulosa ; Angiotensin II ; Mitochondria ; Stereology ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of a chronic treatment with angiotensin II (up to 15 consecutive days) on the mitochondria of the rat zona glomerulosa cells were investigated by electron microscopic and stereological methods. Angiotensin induced a significant increase in the volume of the mitochondrial compartment. Up to the 3rd day of treatment this was due only to the hypertrophy of the organelles, and from the 3rd to the 15th day exclusively to mitochondrial proliferation. The hypothesis that angiotensin controls the growth and proliferation of rat zona glomerulosa mitochondria is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237620
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 48
    Electronic Resource
    Electronic Resource
    Circadian morphometric variation in gastric parietal cell populations of the rat (1980)
    Springer
    Cell & tissue research 211 (1980), S. 175-177 
    Details
    ISSN: 1432-0878
    Keywords: Parietal cell ; Stomach ; Circadian rhythm ; Morphometry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gastric parietal cells of rats maintained under standardized conditions and fed ad libitum were examined by electron microscopy at 6 time points of the 24-h day. Morphometric determinations were made on 4 cell characteristics. The volume density of secretory canaliculi was maximal at the mid-dark sampling point and decreased during the light phase; a secondary peak was seen 1 h before the onset of darkness. The surface density of microvesicles and RER fluctuated inversely with the pattern displayed by secretory canaliculi. The number of multivesicular bodies per cytoplasmic area exhibited a single peak, 1 h after the onset of darkness. It was further noted that parietal cells in the necks and bases of glands differed morphologically and that their organelle populations varied at individual circadian rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233733
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 49
    Electronic Resource
    Electronic Resource
    Neurohormones in the intercellular clefts and in glia-like cells of the rat brain (1980)
    Springer
    Cell & tissue research 211 (1980), S. 251-268 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin ; Luliberin (LRF) ; Intercellular clefts (brain) ; Immunoreactive glia-like cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the aid of electron microscopic immunocytochemistry following the application of antisera against somatostatin and luliberin (LRF), a labeling of the intercellular clefts in different areas of the brain was observed. This labeling is especially conspicuous near the basal pole of the cuboidal ependymal cells, but is also generally present in all regions containing neurohormone-producing perikarya or their processes (for example, the preoptic area, the basal ganglia and the cortex). Furthermore, in all these regions displaying labeled intercellular clefts, glialike cells and sparsely ciliated ependymal cells are found, the secondary lysosomes of which exhibit an immunoreactivity resembling that observed in the intercellular clefts. As sources of the immunoreactive material the following possibilities are discussed: (i) perikarya producing somatostatin or LRF, situated in the wall of the third ventricle and sending fibers between the cuboidal ependymal cells, (ii) hypothalamic and extrahypothalamic projections of both peptidergic systems, and (iii) in the case of somatostatin, immunoreactive perikarya in the cortex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236448
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 50
    Electronic Resource
    Electronic Resource
    The adsorptive and transport capacity of tanycytes during the perinatal period of the rat (1980)
    Springer
    Cell & tissue research 211 (1980), S. 493-501 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamo-hypophysial system ; Rat ; Tanycytes ; Transport ; Ontogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Transport of ferritin and horseradish peroxidase from the 3rd ventricle to the median eminence was examined in rats during the perinatal life, the time when functional interrelations between hypothalamus and hypophysis are established. Protein tracers injected into the lateral ventricle are adsorbed on the apical surface of the tanycyte, mainly on its protrusions or in indentations. On the 18th day of prenatal life a few small bleblike protrusions are observed. After birth microvilli appear. In time their concentration increases to result in an increase of adsorbed substances. They are taken up by smooth and coated pinocytotic vesicles and transported to the basal portion of the cell or to the intercellular space bypassing junctional complexes. In addition to pinocytotic vesicles protein tracers fill channels of smooth ER or Golgi complex and multivesicular bodies illustrating a process probably involved in metabolic or secretory processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234403
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 51
    Electronic Resource
    Electronic Resource
    Localization and neurophysiological properties of motoneurones of the M. triceps surae of the rat after retrograde labelling with evans blue (1980)
    Springer
    Cell & tissue research 212 (1980), S. 73-81 
    Details
    ISSN: 1432-0878
    Keywords: Motoneurones ; Triceps surae muscle ; Retrograde labelling (Evans blue) ; Excitability ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Evans blue was injected into either the medial and lateral gastrocnemius or the soleus muscle of adult albino rats to mark retrogradely the corresponding motoneurones in the spinal cord. The labelled motoneurones were identified by the red fluorescence of their perikarya in the ventral horn of segments L4–L6. In addition, a monosynaptic reflex action potential was recorded only in the ventral roots L4–L6 after stimulation of the nerves to the medial and lateral gastrocnemius and the soleus muscle. Excitability and reflex latencies of labelled and unlabelled motoneurones of segments L4–L6 as well as the conduction velocities of their axons were measured. Apart from a small but statistically nonsignificant increase in excitability, no functional differences were found between labelled and control neurones. Thus, retrograde labelling of motoneurones with Evans blue prior to performing electrophysiological experiments has the advantage that the marked motoneurones can be identified under the fluorescence microscope without the need of additional staining or fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234034
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 52
    Electronic Resource
    Electronic Resource
    Functional difference between “classical” neurosecretory material and vasopressin-like substances of the outer layer of the median eminence (1980)
    Springer
    Cell & tissue research 212 (1980), S. 257-277 
    Details
    ISSN: 1432-0878
    Keywords: CRF ; Vasopressin ; Adrenalectomy ; Rat ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In female Wistar rats the influence of adrenalectomy and NaCl administration on the amount of “classical” neurosecretory material (cNSM) in the supraoptico-hypophysial system and on vasopressin-like substance-containing granules (vlG) in the outer layer of the median eminence has been studied. In conjunction with appropriate sodium replacement, adrenalectomy induces an increase in the amount of vlG but does not alter the amount of cNSM. Administration of hypertonic saline diminishes cNSM but has no or only little influence on the amount of vlG. From the findings it is concluded that cNSM and vlG, in spite of their identical histochemical and immunohistochemical properties, have different functions. The functional significance of the vlG is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233960
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 53
    Electronic Resource
    Electronic Resource
    Two types of luliberin-immunoreactive perikarya in the preoptic area of the rat (1980)
    Springer
    Cell & tissue research 212 (1980), S. 443-455 
    Details
    ISSN: 1432-0878
    Keywords: LRF-immunoreactive perikarya ; Preoptic area ; Light and electron microscopic immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary At the light microscopic level, following immunostaining with a single antiserum against luliberin (LRF), two types of hormone-producing perikarya in the preoptic area are demonstrated. The two cell types differ in their morphological features: a bipolar, smooth-contoured cell type can be differentiated from an irregularly contoured unipolar type. Intermediate forms between both cell types occurring in the same area are not observed. Electron microscopically, both cell types contain labeled granules of similar size and immunoreactivity. It is dicussed whether the uneven surface of the one cell type is due to areas of synaptic contacts, and whether both cell types are integrated in different neuronal and functional circuits. Moreover, at the ultrastructural level, from the irregularly contoured LRF-producing perikarya a further positively stained cell type, probably a glial cell, can be differentiated. The specific labeling of the latter is caused by its content of immunoreactive lysosomal bodies. Differentiation between the labeled glial cells and the irregularly contoured LRF-producing perikarya is not possible at the light microscopic level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236509
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 54
    Electronic Resource
    Electronic Resource
    Differing immunoreactivities of somatostatin in the cortex and the hypothalamus of the rat (1980)
    Springer
    Cell & tissue research 212 (1980), S. 457-464 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin immunoreactivity ; Cortex ; Hypothalamus ; Light and electron microscopic immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using an antibody against somatostatin (antiserum “F”), two somatostatin-immunoreactive systems, (i) a hypothalamic and (ii) an extrahypothalamic cortical system, are demonstrated in the rat. Another antiserum raised against somatostatin (antiserum “BS 102”) stains only the axons but not the perikarya of the hypothalamic system; the cortical somatostatin system does not react with this antiserum. The electron microscopic findings do not allow decision whether the above-mentioned hypothalamic and cortical neurons possess a common prohormonal form of somatostatin, immunoreactive only with antiserum “F”. They show, however, that the granules in both neuronal systems differ considerably; in the cortical neurons they measure approximately 65 nm in diameter, in the hypothalamic neurons 90–120 nm in diameter. Thus, both somatostatin systems are different and independent from one another.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236510
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...