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  • Articles  (44,380)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cytotechnology 34 (2000), S. 131-139 
    ISSN: 1573-0778
    Keywords: apoptosis ; bcl-xL ; cell growth ; cell viability ; hybridoma ; myeloma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract While the ectopic expression of the anti-apoptoticprotein Bcl-2 has been shown to significantly increaseboth cell viability and antibody production in batchculture, some cell lines are refractory to thesemanipulations. For example, the NS/O and theP3x63Ag8.653 murine myelomas, which express highendogenous levels of the Bcl-2 homologue Bcl-xL, areboth resistant to the anti-apoptotic effect of Bcl-2.This indicates that, in these cells, Bcl-2 and Bcl-xLmay be functionally redundant. In order to define therole which Bcl-xL plays in hybridoma cultures, we usedthe Sp2/0-Ag14 cell line. This murine hybridomaexpresses low levels of Bcl-xL and is highly sensitiveto apoptosis induction by cycloheximide (CHX) and byamino acid depletion. Bcl-xL-transfected Sp2/0-Ag14cells were more resistant than the wild type and theplasmid-containing cells to apoptosis induced by CHXand by glutamine depletion. Moreover, when compared tothe vector-transfected control, Bcl-xL-Sp2/0 cellsexhibited a substantial increase in viability instationary batch culture. Interestingly, Sp2/0-Ag14cells overexpressing Bcl-xL showed a growth behaviourthat was similar to the parent myeloma cell lineP3x63Ag8.653. Our results suggest that Bcl-xLexpression levels are sufficient to account for therelative robustness of some hybridoma cell lines instationary batch cultures.
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  • 2
    ISSN: 1573-0778
    Keywords: glucose ; glutamine ; hybridoma ; nucleotides oxygen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0–25 mM) and glutamine (0–9 mM). The intracellularconcentrations of ATP, GTP, UTP and CTP, and theadenylate energy charge increased concomitantly withthe medium glucose concentration. The total adenylatenucleotide concentration did not change over a glucose concentration range of 1–25 mM but therelative levels of AMP, ADP and ATP changed as theenergy charge increased from 0.36 to 0.96. Themaximum oxygen uptake rate (OUR) was obtained in thepresence of 0.1–1 mM glucose. However at glucoseconcentrations 〉1 mM the OUR decreased suggestinga lower level of aerobic metabolism as a result of theCrabtree effect.A low concentration of glutamine (0.5 mM) caused asignificant increase (45–128%) in the ATP, GTP,CTP, UTP, UDP-GNac, and NAD pools and a doubling ofthe OUR compared to glutamine-free cultures. Theminimal concentration of glutamine also caused anincrease in the total adenylate pool indicating thatthe amino acid may stimulate thede novosynthesis of nucleotides. However, all nucleotidepools and the OUR remained unchanged within the rangeof 0.5–9 mM glutamine.Glucose was shown to be the major substrate forenergy metabolism. It was estimated that in thepresence of high concentrations of glucose (10–25 mM),glutamine provided the energy for the maintenance ofup to 28% of the intracellular ATP pool, whereas theremainder was provided by glucose metabolism.
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  • 3
    ISSN: 1573-0778
    Keywords: antibody production ; human monoclonal antibody ; hybridoma ; retinoic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The enhancement of human monoclonal antibody production by retinoic acid (RA) was evaluated usingthe human-human hybridoma cell line BD9 underserum-free culture condition. The amount of humanIgG secreted by BD9 hybriodmas was enhanced abouteight-fold by treatment with 10-7 M of RA for 4days. Northern blot analysis showed that both mRNAlevels of the IgG light and heavy chains were markedlyincreased by RA when compared with control without RAtreatment. On the other hand, it was found thatcontinuous treatment of cells with RA was not alwaysrequired to exhibit the enhancing effect, suggestingthat RA may act as a trigger for IgG gene expression. The comparison between extra- and intracellular IgGamounts by immunoblot analysis suggests that thesecretion rate of IgG may be accelerated by RAtreatment. These results suggest that RA may be aneffective culture additive for efficient production ofhuman monoclonal antibody using human-humanhybridomas.
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  • 4
    ISSN: 1573-0778
    Keywords: hybridoma ; glutamate ; cystine ; transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Glutamic acid was found to be growth inhibitory to a murinelymphocyte hybridoma in a concentration-dependent manner from 3to 12 mM glutamate. At 12 mM glutamate there was a 70% decreasein the specific growth rate of the cells. Attempts to alleviateinhibition or adapt cells to growth in glutamate-based mediawere unsuccessful. It is proposed that elevated glutamate levelsimpair adequate uptake of cystine, a critical amino acid for thesynthesis of glutathione. Glutathione is required by cells toprevent intracellular oxidative stress. The measured rate ofuptake of U-14C L-cystine into the cells was found to havethe following parameters: Km = 0.87 mM, Vmax = 0.9nmole/mg cell protein per min. The uptake was sodiumindependent and resembled the previously described x- ctransport system, with elevated glutamate levels causingextensive inhibition. Glutamate at a concentration of 1.4 mMcaused a 50% decrease in cystine uptake from the serum-freegrowth medium. Glutamate was taken up from the external medium(Km = 20 mM and Vmax = 12.5 nmole/mg cell protein permin) by the same transport system in a stereo specific, sodiumindependent manner. Of the amino acids examined, it was foundthat cystine and homocysteic acid were the most extensiveinhibitors of glutamate uptake and that inhibition was competitive. Metabolic profiles of the cells grown in culturescontaining enhanced glutamate levels revealed an overallincrease in net production of alanine, serine, asparagine andaspartate. A substantially increased specific consumption ofglutamate was accompanied by a decreased consumption of cystine,valine and phenylalanine.The combined kinetic and metabolic results indicate thatglutamate and cystine are taken up by the anionic transportsystem x- c. The increasing levels of glutamate in themedium result in a decreased transport of cystine by this systemdue to competitive inhibition by glutamate.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Neuroscience and behavioral physiology 30 (2000), S. 357-363 
    ISSN: 1573-899X
    Keywords: Anoxia ; brain slices ; calcium ; phosphoinositides ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The involvement of the calcium and phosphoinositide intracellular regulatory systems in the molecular-cellular mechanisms of adaptation of the brain to hypoxia induced by transient anoxia were studied in slices of rat olfactory cortex. Anoxia lasting 2 min initiated the development of moderate but stable activation of intracellular regulatory systems during the reoxygenation period, with increases in binding of Ca2+ to intracellular hydrophobic domains and increases in the level of polyphosphoiositide metabolism. During this period, cells in the slices released neuromediator factors into the perfusion fluid; transfer of these to recipient slices induced similar changes in the activities of intracellular regulatory system components in the recipient slices. After anoxia lasting 10 min, NMDA-mediated pathogenic hyperactivity of the calcium and phosphoinositide systems developed. Preliminary moderate activation of these systems by transient anoxia or neuromodulator factors released by cells in response to transient anoxia prevented disruption of intracellular regulatory system activity induced by subsequent longer-lasting anoxia.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Neuroscience and behavioral physiology 30 (2000), S. 243-254 
    ISSN: 1573-899X
    Keywords: Volume transmission ; adaptation ; peptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract This review presents published data and results from our own studies providing evidence for the important role of volume, non-synaptic transmission of adaptogenic signals in the mechanisms forming the long-term adaptive reactions of the brain. The importance of chemical factors involved in volume transmission and secreted by cells in this process is discussed. Special attention is paid to peptides—possible mediators of volume transmission of adaptive-type signals. Evidence has been obtained for the presence of peptides and their role in the mechanism of development of adaptive brain reactions of different origins, especially those arising in response to tetanic stimulation of neurons and transient hypoxic stress. An original method for testing for the effects of neuromodulator factors released by cells in donor slices subjected to these treatments on recipient slices was used to show that these factors had pronounced effects on synaptic transmission and could induce long-term potentiation of synaptic transmission, protecting against functional derangements due to prolonged anoxia. Blockade of protein synthesis in donor slices subjected to adaptogenic treatments suppressed the appearance of these effects. The review concludes with a discussion of the mechanisms of interaction of the synaptic and volume transmission of signals involved in forming long-term adaptive brain reactions.
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  • 7
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    Springer
    Annals of biomedical engineering 28 (2000), S. 1194-1199 
    ISSN: 1573-9686
    Keywords: Cancellous bone ; stress ; strain ; strength ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The statistical distribution of von Mises stress in the trabeculae of human vertebral cancellous bone was estimated using large-scale finite element models. The goal was to test the hypothesis that average trabecular von Mises stress is correlated to the maximum trabecular level von Mises stress. The hypothesis was proposed to explain the close experimental correlation between apparent strength and stiffness of human cancellous bone tissue. A three-parameter Weibull function described the probability distribution of the estimated von Mises stress (r2) 〉 0.99 for each of 23 cases). The mean von Mises stress was linearly related to the standard deviation (r2=0.63) supporting the hypothesis that average and maximum magnitude stress would be correlated. The coefficient of variation (COV) of the von Mises stress was nonlinearly related to apparent compressive strength, apparent stiffness, and bone volume fraction (adjusted r2=0.66, 0.56, 0.54, respectively) by a saturating exponential function [COV=A+B exp(−x/C)]. The COV of the stress was higher for low volume fraction tissue (〈0.12) consistent with the weakness of low volume fraction tissue and suggesting that stress variation is better controlled in higher volume fraction tissue. We propose that the average stress and standard deviation of the stress are both controlled by bone remodeling in response to applied loading. © 2000 Biomedical Engineering Society. PAC00: 8719Rr
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 341-344 
    ISSN: 1573-0972
    Keywords: Composite ; fermentation ; finger millet ; skim milk
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Fermented composite beverages of finger millet and milk are popular, nutritious, traditional foods in many parts of Zimbabwe. With the aim of commercial production, we determined what type of microbial cultures can be used to ferment a composite finger millet and skimmed milk powder gruel and the optimum conditions for its production. Composites containing between 0 and 100% finger millet gruel by volume were inoculated and incubated at various temperatures. The desired pH of 4.5 or less was obtained with incubation at 30 to 45 °C (but not at lower temperatures) with lower pH values being obtained as the temperature increased. YC380 (a yoghurt type bacterial starter culture) produced a pH of 4.5 or less only when skim milk was also present; V2 (another yoghurt type bacterial starter culture) did so at all levels of finger millet gruel and JC (a mixed strain culture developed to ferment cereals) only when finger millet gruel was present. A clear relationship between incubation temperature and syneresis could not be established but syneresis decreased significantly (p 〈 0.05) with increasing proportions of finger millet gruel. A thick product with a set consistency was obtained with YC380 at an incubation temperature of 45 °C and a storage temperature of 7 °C regardless of proportion of finger millet gruel. V2 produced a thick product with a set consistency at an incubation temperature of 45 °C, and storage temperature of 7 °C and when the proportions of finger millet gruel were between 0 and 50%. It appears that yoghurt type bacterial cultures can be successfully used to produce a composite fermented beverage from finger millet and skim milk, but cultures developed for fermentation of cereals are not suitable.
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  • 9
    ISSN: 1573-0778
    Keywords: fed batch ; hybridoma ; macromolecular composition ; monoclonal antibody ; substrate limitation ; target specific growth rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Batch and fed-batch cultures of a murine hybridomacell line (AFP-27) were performed in a stirred tankreactor to estimate the effect of feed rate on growthrate, macromolecular metabolism and antibodyproduction. Macromolecular composition was foundto change dynamically during batch culture ofhybridoma cells possibly due to active production ofDNA, RNA and protein during the exponential phase.Antibody synthesis is expected to compete with theproduction of cellular proteins from the amino acidpool. Therefore, it is necessary to examine therelationship between cell growth in terms of cellularmacromolecules and antibody production. In this study,we searched for an optimum feeding strategy bychanging the target specific growth rate in fed-batchculture to give higher antibody productivity whileexamining the macromolecular composition. Concentratedglucose (60 mM) and glutamine (20 mM) in DR medium(1:1 mixture of DMEM and RPMI) with additional aminoacids were fed continuously to the culture and thefeed rate was updated after every sampling to ensureexponential feeding (or approximately constantspecific growth rate). Specific antibody productionrate was found to be significantly increased in thefed-batch cultures at the near-zero specific growthrate in which the productions of cellular DNA, RNA,protein and polysaccharide were strictly limited byslow feeding of glucose, glutamine and other nutrients. Possible implications of these results are discussed.
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  • 10
    ISSN: 1573-0778
    Keywords: cell culture ; hollow fiber bioreactor ; hybridoma ; micro bioreactor ; optimization ; T-flask
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In this article, cell growth in a novel micro hollow fiberbioreactor was compared to that in a T-flask and theAcuSyst-Maximizer®, a large scale industrial hollowfiber bioreactor system. In T-flasks, there was relativelylittle difference in the growth rates of one murine hybridomacultured in three different media and for three other murinehybridomas cultured in one medium. However, substantialdifferences were seen in the growth rates of cells in themicro bioreactor under these same conditions. These differencecorrelated well with the corresponding rates of initial cellexpansion in the Maximizer. Quantitative prediction of thesteady-state antibody production rate in the Maximizer was moreproblematic. However, conditions which lead to faster initialcell growth and higher viable cell densities in the microbioreactor correlated with better performance of a cell line inthe Maximizer. These results demonstrate that the microbioreactor is more useful than a T-flask for determining optimalconditions for cell growth in a large scale hollow fiberbioreactor system.
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  • 11
    Electronic Resource
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    Springer
    Molecular and cellular biochemistry 196 (1999), S. 31-42 
    ISSN: 1573-4919
    Keywords: thiols ; antioxidant ; redox ; adaptation ; oxidative stress ; skeletal muscle ; dietary supplement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Glutathione plays a central role in the maintenance of tissue antioxidant defenses and in the regulation of redox sensitive signal transduction. In muscle cells, the level and redox status of GSH regulates activity of the redox sensitive transcription factor NF-κB. Physical exercise may cause oxidation of GSH in tissues such as the blood, skeletal muscle and liver. Endurance training strengthened GSH dependent tissue antioxidant defenses in most studies. Although studies investigating the effect of sprint training are few, current results show that sprint training may also have a beneficial effect on tissue GSH homeostasis. Skeletal muscle GSH level appears to be tightly regulated by the state of physical activity. Regular exercise enhances and chronic inactivity decreases the level of GSH in this tissue. N-acetyl-L-cysteine (NAC) and α-lipoic acid (LA) are two antioxidant dietary supplements that are able to enhance cellular GSH levels. Because LA can be recycled to its potent dithiol form, dihydrolipoate, by enzymes present in the human cell it has a clear advantage over NAC. Recently an improved form of LA, a positively charged analogue (LA-Plus), has been discovered. LA-Plus has more potent immuno-modulatory activity compared to LA. Both LA and NAC have been shown to have beneficial effects in protecting tissue GSH homeostasis against exercise induced oxidative stress.
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  • 12
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    Springer
    Biology and philosophy 14 (1999), S. 65-82 
    ISSN: 1572-8404
    Keywords: adaptation ; explanation ; evolution ; preadaptation ; specialization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Philosophy
    Notes: Abstract The concept of preadaptation, though useful, continues to trouble evolutionary scientists. Usually, it is treated as if it were really adaptation, prompting such diverse theorists as Gould and Vrba, and Dennett to suggest its removal from evolutionary theory altogether. In this paper, I argue that the as-if sense is ill-founded, and that the sense of preadaptation as a process may be defended as unequivocal and generally useful in evolutionary explanations, even in such problem areas as human evolution.
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  • 13
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    Biology and philosophy 14 (1999), S. 211-233 
    ISSN: 1572-8404
    Keywords: adaptation ; evolutionary psychology ; reasoning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Philosophy
    Notes: Abstract I discuss two types of evidential problems with the most widely touted experiments in evolutionary psychology, those performed by Leda Cosmides and interpreted by Cosmides and John Tooby. First, and despite Cosmides and Tooby's claims to the contrary, these experiments don't fulfil the standards of evidence of evolutionary biology. Second Cosmides and Tooby claim to have performed a crucial experiment, and to have eliminated rival approaches. Though they claim that their results are consistent with their theory but contradictory to the leading non-evolutionary alternative, Pragmatic Reasoning Schemas theory, I argue that this claim is unsupported. In addition, some of Cosmides and Tooby's interpretations arise from misguided and simplistic understandings of evolutionary biology. While I endorse the incorporation of evolutionary approaches into psychology, I reject the claims of Cosmides and Tooby that a modular approach is the only one supported by evolutionary biology. Lewontin's critical examinations of the applications of adaptationist thinking provide a background of evidentiary standards against which to view the currently fashionable claims of evolutionary psychology.
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  • 14
    ISSN: 1573-0778
    Keywords: apoptosis ; Bcl-2 ; fixed-bed ; hollow fibre ; hybridoma ; perfusion ; protein-free medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Apoptosis is an active, genetically determined death mechanism which can be induced by a wide range of physiological factors and by mild stress. It is the predominant form of cell death during the production of antibodies from murine hybridoma cell lines. A number of studies have now demonstrated that the suppression of this death pathway, by means of over-expression of survival genes such as bcl-2, results in improved cellular robustness and antibody productivity during batch culture. In the present study, the influence of bcl-2 expression on hybridoma productivity in two high density perfusion bioreactor systems was investigated. In the first system, a fixed-bed reactor, the DNA content in the spent medium was 25% higher in the control (TB/C3-pEF) culture than that found in the bcl-2 transfected (TB/C3-bcl2) cultures at all perfusion rates. This is indicative of a higher level of cell death in the control cell line. The average antibody concentration for the TB/C3-pEF cell line was 14.9 mg L-1 at perfusion rates of 2.6 and 5.2 d-1. However, for the TB/C3-bcl2 cell line it was 33 mg L-1 at dilution rates of 2 and 4 d-1. A substantial increase in antibody concentration was also found in the Integra Tecnomouse hollow fibre reactor. The antibody titre in the TB/C3-bcl2 cassette was nearly 100% higher than that in the TB/C3-pEF cassette during the cultivation period which lasted 6 weeks. Clearly, these results demonstrate the positive impact of bcl-2 over-expression on production of antibody in hybridoma perfusion cultures.
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  • 15
    ISSN: 1573-0778
    Keywords: batch culture ; conditioned medium ; growth ; hybridoma ; inoculum ; protein productivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Apart from gas concentrations, temperature, and pH, generally only the initial conditions can be manipulated in batch culture. Inoculum size and initial conditioned medium concentration represent two important considerations for optimal batch production. Two hybridoma cell lines were used to assess the impact of these initial conditions on population growth and monoclonal antibody productivity in suspension batch culture. Varying initial cell concentration over the range of 1.0 × 105 cells mL-1 to 3.0 × 105 cells mL-1 did not affect maximum product titre or maximum volumetric cell-hours attained. Initial percent of conditioned medium up to 40 percent strongly impacted on population growth and productivity, with initial levels of 30 to 40% conditioned medium reducing or eliminating lag phase and increasing average viable cell density. However, specific productivity and product titre declined with increasing initial percent conditioned medium, even on a per volume of fresh medium basis. Glutamine and glucose depletion or ammonia toxicity could cause depressed product titres when conditioned medium is used. Glutamine and glucose levels can easily be replenished in conditioned medium at minimal cost, and ammonia can be removed. Specific productivity was higher during cyclic batch operating mode than during batch operating mode. This may be because cyclic batch operating mode results in an incidental volume of conditioned medium at the beginning of each cycle. A two stage, cyclic-batch/batch operating mode can be employed to fully utilize medium and maximize product titre.
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  • 16
    ISSN: 1573-0778
    Keywords: antibody productivity ; apoptosis ; BAG-1 ; Bcl-2 ; cell survival ; hybridoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Human bcl-2 and bag-1 DNA were introduced into mouse hybridoma 2E3- O cells and expressed. The expression of bcl-2 in BCMGneo-bcl2 transfectants was confirmed by ELISA and that of bag-1 in pZeo-bag1 was confirmed by western blotting. In batch cultures, the over-expression of bcl-2 prolonged the culture period by 2 days and co-expression of bcl-2 and bag-1 prolonged the culture period by 3 days. The delayed increase in the dead cell number in culture of the bcl-2 and bag-1 cotransfectant indicated the additional antiapoptosis effect of bcl-2 and bag-1 cotransfection in comparison with the bcl-2 only transfection. The bcl-2 transfectants (2E3O-Bcl2) produced antibody twofold per batch culture in comparison with 2E3-O cells transfected with BCMGSneo (2E3O-Mock). Enhancement of this MoAb production was due to the improved survival of the cells and was not due to stimulation of antibody production rate per cell by Bcl-2 expression. And the bcl-2 and bag-1 co-transfectant (2E3O-Bcl2-BAG1) produced antibody approximately fourfold of 2E3O-Mock per batch culture. Enhancement of this MoAb production was due to the improved survival of the cells and was partly due to stimulation of MoAb production rate per cell in the non-growing phase by the cotransfection. The method to engineer hybridoma cells genetically with bcl-2 and bag-1 for increasing viability and productivity would be widely applied for improving antibody productivity of hybridoma cultures.
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  • 17
    ISSN: 1573-675X
    Keywords: Apoptosis ; bcl-2 ; Ca2+ ; flow cytometry ; hybridoma ; K+ ; Mg2+ ; necrosis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have addressed the possibility that Ca2+, Mg2+ and K+ ions play a central role in governing the morphological and biochemical changes attributed to apoptotic cell death. By removing Ca2+, Mg2+ or K+ ions from the cell culture medium we were able to assess the contribution of each ion to hybridoma cell growth and viability. The differences were explained in terms of a possible reduction in their respective intracellular levels. From several lines of evidence, the deprivation of K+ ions was the most detrimental to cellular growth and viability and induced significant levels of early apoptotic cells. Another effect of this deprivation was to weaken the plasma membranes without causing membrane breakdown; exposure to high agitation rates confirmed fragility of the cell membranes. Removal of Mg2+ caused a reduction in the levels of early apoptotic cells and predisposed cells to high levels of primary necrotic death. The lower levels of apoptotic cells failed to demonstrate the classic nuclear morphology associated with apoptosis, while retaining other apoptotic features. These results highlighted the importance of utilizing several assays for the determination of apoptosis. The absence of Ca2+ appeared to be the mildest insult, but its deprivation did accelerate a significant decline in culture by increasing apoptotic death. Hybridoma cells overexpressing the apoptotic suppresser gene bcl-2 were protected from the predominantly necrosis inducing effects of Mg2+ ion deprivation and apoptosis inducing effects of Ca2+ ion deprivation. However, apoptosis was not as effectively suppressed in bcl-2 cells responding to incubation in K+ free medium. The inclusion of bcl-2 activity in the mechanisms of Ca2+ Mg2+ or K+ deprivation induced cell death emphasizes a close relationship between ionic dissipation and the apoptotic process.
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  • 18
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    Journal of computational neuroscience 6 (1999), S. 99-120 
    ISSN: 1573-6873
    Keywords: adaptation ; auditory cortex ; change detection ; lateral inhibition ; mismatch negativity ; MMN
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Medicine , Physics
    Notes: Abstract We offer a model of how human cortex detects changes in the auditory environment. Auditory change detection has recently been the object of intense investigation via the mismatch negativity (MMN). MMN is a preattentive response to sudden changes in stimulation, measured noninvasively in the electroencephalogram (EEG) and the magnetoencephalogram (MEG). It is elicited in the oddball paradigm, where infrequent deviant tones intersperse a series of repetitive standard tones. However, little apart from the participation of tonotopically organized auditory cortex is known about the neural mechanisms underlying change detection and the MMN. In the present study, we investigate how poststimulus inhibition might account for MMN and compare the effects of adaptation with those of lateral inhibition in a model describing tonotopically organized cortex. To test the predictions of our model, we performed MEG and EEG measurements on human subjects and used both small- (〈1/3 octave) and large- (〉5 octaves) frequency differences between the standard and deviant tones. The experimental results bear out the prediction that MMN is due to both adaptation and lateral inhibition. Finally, we suggest that MMN might serve as a probe of what stimulus features are mapped by human auditory cortex.
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  • 19
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    Journal of computational neuroscience 7 (1999), S. 55-70 
    ISSN: 1573-6873
    Keywords: habituation ; adaptation ; vestibulo-ocular reflex ; goldfish ; linear systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Medicine , Physics
    Notes: Abstract Modification of the vestibulo-ocular reflex (VOR) by vestibular habituation is an important paradigm in the study of neural plasticity. The VOR is responsible for rotating the eyes to maintain the direction of gaze during head rotation. The response of the VOR to sinusoidal rotation is quantified by its gain (eye rotational velocity/head rotational velocity) and phase difference (eye velocity phase—inverted head velocity phase). The frequency response of the VOR in naïve animals has been previously modeled as a high-pass filter (HPF). A HPF passes signals above its corner frequency with gain 1 and phase 0 but decreases gain and increases phase lead (positive phase difference) as signal frequency decreases below its corner frequency. Modification of the VOR by habituation occurs after prolonged low-frequency rotation in the dark. Habituation causes a reduction in low-frequency VOR gain and has been simulated by increasing the corner frequency of the HPF model. This decreases gain not only at the habituating frequency but further decreases gain at all frequencies below the new corner frequency. It also causes phase lead to increase at all frequencies below the new corner frequency (up to some asymptotic value). We show that habituation of the goldfish VOR is not a broad frequency phenomena but is frequency specific. A decrease in VOR gain is produced primarily at the habituating frequency, and there is an increase in phase lead at nearby higher frequencies and a decrease in phase lead at nearby lower frequencies (phase crossover). Both the phase crossover and the frequency specific gain decrease make it impossible to simulate habituation of the VOR simply by increasing the corner frequency of the HPF model. The simplest way to simulate our data is to subtract the output of a band-pass filter (BPF) from the output of the HPF model of the naïve VOR. A BPF passes signals over a limited frequency range only. A BPF decreases gain and imparts a phase lag and lead, respectively, as frequency increases and decreases outside this range. Our model produces both the specific decrease in gain at the habituating frequency, and the phase crossover centered on the frequency of habituation. Our results suggest that VOR habituation may be similar to VOR adaptation (in which VOR modification is produced by visual-vestibular mismatch) in that both are frequency-specific phenomena.
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  • 20
    ISSN: 1573-0972
    Keywords: Conidia ; cultures ; fermentation ; G. fujikuroi ; morphological ; strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Strain H-984 of G. fujikuroi grown for 38 h in a shake flask with medium containing 20 g glucose l−1, 3 g yeast extract l−1, 2.5 g NH4NO3 l−1, 0.5 g KH2PO4 l−1, 0.1 g MgSO4 l−1, 1 g CaCO3 l−1, and inoculated into a bioreactor with medium containing 60 g glucose l−1; 1 g NH4Cl l−1; 3 g KH2PO4 l−1 and 1.5 g MgSO4 l−1 produced 1100 mg gibberellic acid l−1.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 25-41 
    ISSN: 1573-1596
    Keywords: adaptation ; agriculture ; climate change ; Kazakhstan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Agriculture in Kazakhstan is sensitive to climate, and wheat yields could be reduced up to 70% under climate change. With the transition from a socialist economy to a free market economy, decisions are being made now that will affect Kazakhstan's ability to cope with climate change. A team of Kazakh and American researchers examined the cost-effectiveness and barriers to implementations of adaptation options for climate change. Twelve adaptation options that increase flexibility to respond to climate change were identified using a screening matrix. Four options, forecasting pest outbreaks, developing regional centers for preserving genetic diversity of seeds, supporting a transition to a free market, and reducing soil erosion through the use of changed farming practices, were examined. The Adaptation Decision Matrix (ADM) was then applied to estimate benefits using expert judgment (using an arbitrary numerical scale, not monetary values) and benefits estimates were compared to costs to determine cost-effectiveness. The ADM uses subjective measures of how well adaptation options meet policy objectives. Controlling soil erosion was estimated to have the highest benefits, but the high costs of implementation appears to make it relatively cost-ineffective. Supporting a transition to a free market was ranked as the most cost-effective measure, with regional centers second. However, use of different scales to quantify benefits or different weights can result in regional centers being more cost-effective than the transition to a free market. Regional centers was also judged to have fewer barriers to implementation than a transition to a free market. These results will be incorporated in Kazakhstan's National Action Plan. The ADM and other tools are relatively easy to apply, but are quite subjective and difficult to evaluate. The tools can be quite useful by decision makers to analyze advantages and disadvantages between different adaptation options, but should be supplemented with additional, particularly quantitative analysis.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 137-165 
    ISSN: 1573-1596
    Keywords: adaptation ; agriculture ; climate change ; decision-making ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This work presents a framework for viewing agricultural adaptation, emphasizing the multiple spatial and temporal scales on which individuals and institutions process information on changes in their environment. The framework is offered as a means to gain perspective on the role of climate variability and change in agricultural adaptation, and developed for a case study of Australian agriculture. To study adaptation issues at the scale of individual farms we developed a simple modelling framework. The model highlights the decision making element of adaptation in light of uncertainty, and underscores the importance of decision information related to climate variability. Model results show that the assumption of perfect information for farmers systematically overpredicts adaptive performance. The results also suggest that farmers who make tactical planting decisions on the basis of historical climate information are outperformed by those who use even moderately successful seasonal forecast information. Analysis at continental scales highlights the prominent role of the decline in economic operating conditions on Australian agriculture. Examples from segments of the agricultural industry in Australia are given to illustrate the importance of appropriate scale attribution in adapting to environmental changes. In particular, adaptations oriented toward short time scale changes in the farming environment (droughts, market fluctuations) can be limited in their efficacy by constraints imposed by broad changes in the soil/water base and economic environment occuring over longer time scales. The case study also makes the point that adaptation must be defined in reference to some goal, which is ultimately a social and political exercise. Overall, this study highlights the importance of allowing more complexity (limited information, risk aversion, cross-scale interactions, mis-attribution of cause and effect, background context, identification of goals) in representing adaptation processes in climate change studies.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 199-213 
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; impact assessment ; response options ; vulnerability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper outlines what is meant by "adaptation" to climate change, and how it might be addressed in the IPCC Assessments. Two roles of adaptation in the climate change field are identified: adaptation as part of impact assessment (where the key question is: what adaptations are likely?), and adaptation as part of the policy response (where the central question is: what adaptations are recommended?). The concept of adaptation has been adopted in several fields including climate impact assessment and policy development, risk management, and natural hazards research. A framework for systematically defining adaptations is based on three questions: (i) adaptation to what? (ii) who or what adapts? and (iii) how does adaptation occur? The paper demonstrates that, for adaptation purposes, climate extremes and variability are integral parts of climate change, along with shifts in mean conditions. Attributes for differentiating adaptations include purposefulness, timing, temporal and spatial scope, effects, form and performance. The framework provides a guide for the treatment of adaptation in the IPCC assessments, both in the assessment of impacts and in the evaluation of adaptive policy options.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 215-225 
    ISSN: 1573-1596
    Keywords: climate ; adaptation ; impacts
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    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract There is a critical need to collectively understand, to develop adaptation options to enhance the benefits, and to reduce the social and economic vulnerabilities induced by climate variability and change. This paper uses key questions to help build a framework for adaptation by first organizing the questions into adaptation science, management and option components, including their respective sub-categories. The process of adaptation depends on many factors, including who or what adapts, what they adapt to, how they adapt and what and how resources are used. This conceptual model is designed to organize concepts regarding adaptation, to help stimulate ideas, and to explore the linkages among parts of the adaptation cycle. Predictive models need to be developed to determine the outcomes of planned adaptation strategies. For the best and most realistic evaluation of climate problems, adaptation and impacts should be considered together. This joint approach improves the assessment of the significance and dangers of the current and future climate, as well as the determination of solutions (e.g., how to prepare for a changing climate) and their priorities. Challenges of adaptive management are discussed in terms of a framework with linkages to adaptation science and options. Adaptation research and applications work continue to build on the foundation of science and management frameworks to address the risks and uncertainties in the decision-making process and to identify adaptation options.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 227-237 
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; climate variability ; data ; climate applications ; El Niño ; UNFCCC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract An extensive foundation of high quality data and information on the climate and on the biological, environmental and social systems affected by climate is required in order to understand the climate impact processes involved, to develop new adaptation practices, and to subsequently implement these practices. Experience of the impacts of current and past variability of climate and sea level is a prime source of information. Many practices are in use to reduce climate impacts, for example in engineering design, agricultural risk management and climate prediction services, though their roles as adaptations to climate change are not widely appreciated. While there are good data sets on some factors and in some regions, in many cases the databases are inadequate and there are few data sets on adaptation-specific quantities such as vulnerability, resilience and adaptation effectiveness. Current international action under the United Nations Framework Convention on Climate Change (UNFCCC) pays little attention to adaptation and its information requirements. Furthermore there are trends toward reduced data gathering and to restrictions on access to data sets, especially arising from cost and commercialisation pressures. To effectively respond to the changes in climate that are now inevitable, governments will need to more clearly identify adaptation as a central feature of climate change policy and make a renewed shared commitment to collecting and freely exchanging the necessary data.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 239-252 
    ISSN: 1573-1596
    Keywords: climate change ; coastal zones ; adaptation ; vulnerability ; IPCC Technical Guidelines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper evaluates the IPCC Technical Guidelines for Assessing Climate Change Impacts and Adaptations with respect to the guidance offered for coastal-adaptation assessment. It appears that the IPCC Technical Guidelines focus strongly on implementation. This paper uses both conceptual and empirical information is used in this paper to show that coastal adaptation embraces more than selecting one of the "technical" options to respond to sea-level rise (retreat, accommodate or protect). Coastal adaptation is a more complex and iterative process with a series of policy cycles. To be effective, an expanded adapta-tion framework involving four steps is suggested, including (i) information collection and awareness raising; (ii) planning and design; (iii) implementation; and (iv) monitoring and evaluation. The incom-plete coverage of these four steps in existing coastal-adaptation assessments constrains the development of adaptation strategies that are supported by the relevant actors and integrated into existing management. Researchers and policy-makers are recommended to work together to establish a framework for adaptation that is integrated within current coastal management processes and practices and takes a broader view on the subject.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 319-329 
    ISSN: 1573-1596
    Keywords: uncertainty ; risk ; adaptation ; extreme events ; (credible) information ; integrated assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper draws ten lessons from analyses of adaptation to climate change under conditions of risk and uncertainty: (1) Socio-economic systems will likely respond most to extreme realizations of climate change. (2) Systems have been responding to variations in climate for centuries. (3) Future change will effect future citizens and their institutions. (4) Human systems can be the sources of surprise. (5) Perceptions of risk depend upon welfare valuations that depend upon expectations. (6) Adaptive decisions will be made in response to climate change and climate change policy. (7) Analysis of adaptive decisions should recognize the second-best context of those decisions. (8) Climate change offers opportunity as well as risk. (9) All plausible futures should be explored. (10) Multiple methodological approaches should be accommodated. These lessons support two pieces of advice for the Third Assessment Report: (1) Work toward consensus, but not at the expense of thorough examination and reporting of the "tails" of the distributions of the future. (2) Integrated assessment is only one unifying methodology; others that can better accommodate those tails should be encouraged and embraced.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 307-318 
    ISSN: 1573-1596
    Keywords: adaptation ; climatic change ; economic welfare ; costs and benefits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential damages of climate change and climate variability are dependent upon the responses or adaptations that people make to their changing environment. By adapting the management of resources, the mix and methods of producing goods and services, choices of leisure activities, and other behavior, people can lessen the damages that would otherwise result. A framework for assessing the benefits and costs of adaptation to both climate change and climate variability is described in the paper. The framework is also suitable for evaluating the economic welfare effects of climate change, allowing for autonomous adaptation by private agents. The paper also briefly addresses complications introduced by uncertainty regarding the benefits of adaptation and irreversibility of investments in adaptation. When investment costs are irreversible and there is uncertainty about benefits, the usual net present value criterion for evaluating the investment gives the wrong decision. If delaying an adaptation project is possible, and if delay will permit learning about future benefits of adaptation, it may be preferable to delay the project even if the expected net present value is positive. Implications of this result for adaptation policy are discussed in the paper.
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    Mitigation and adaptation strategies for global change 4 (1999), S. 343-361 
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; socioeconomic impacts ; Egypt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Assessment of the vulnerability and expected socioeconomic losses over the Nile delta coast due to the impact of sea level rise is carried out in details. Impacts of sea level rise over the Governorates of Alexandria and Port Said in particular, are evaluated quantitatively. Analysis of the results at Alexandria Governorate indicate that, if no action is taken, an area of about 30% of the city will be lost due to inundation. Almost 2 million people will have to abandon their homeland; 195,000 jobs will be lost and an economic loss of over $3.5 Billion is expected over the next century. At Port Said Governorate results indicate that beach areas are most severely affected (hence tourism), followed by urban areas. The agriculture sector is the least affected sector. It is estimated that the economic loss is over $ 2.0 Billion for 0.50 m SLR and may exceed $ 4.4 Billion for 1.25 m SLR. Options and costs of adaptation are analyzed and presented. Multi-criteria and decision matrix approaches, based on questionnaire surveys are carried out to identify priorities for the two cases. Analysis of these techniques of two options; the current policy (hard protection measures on some vulnerable areas) and no action (stopping these activities) have the lowest scores. Beach nourishment and integrated coastal zone management (ICZM) have the highest scores, however ICZM has high cost measures. The most cost-effective option is the land-use change, however with relatively very high cost measure. It is recommended that an ICZM approach be adopted since it provides a reasonable trade off between costs and cost effectiveness.
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    Cytotechnology 30 (1999), S. 27-36 
    ISSN: 1573-0778
    Keywords: agitation ; fatty acids ; hybridoma ; linoleic acid ; lipid
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    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The murine hybridoma (CC9C10) was subjected to high shear rates in a spinner flask to determine the effect of various culture additives on cell survival. At 500 rpm, the half-life of the viable cell concentration in a low protein serum-free medium was 50 min. Both bovine serum albumin and Pluronic F-68 had a significant effect in protecting cells under these conditions. The effects of the two supplements were additive, so that in the presence of both supplements there was minimal cell damage at 500 rpm. The survival rate of cells grown in media supplemented with linoleic acid improved significantly under high stirring rates. Cells grown for one passage in 50 μM linoleic acid and stirred at 500 rpm had a significantly higher survival rate than control cells. For cells grown over 5 passages in 25 μM linoleic acid, the survival rate at 470 rpm was ×3 greater than that determined for control cells. This difference gradually decreased at higher stirring rates up to 610 rpm when the half-life of the viable cell population was reduced to ∼10 min. Supplementation of cultures with linoleic acid has previously been shown to result in incorporation into all three cellular lipid fractions - polar, non-polar and free fatty acid (Butler et al., 1997). Our explanation for the increased survivability of the cells at high agitation rates in the presence of linoleic acid is that the structural lipid components of the cell including the outer membrane attained a higher unsaturated/saturated ratio which was more robust than that of control cells.
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    ISSN: 1573-0778
    Keywords: antibody production ; human monoclonalantibody ; hybridoma ; lung cancer ; vitamin A acetate
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    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The antibody productivity of the human–human hybridoma cell line AE6, which produces the lung cancer specific human monoclonal antibody AE6F4, was enhanced fourfold upon stimulation with 1 μg/ml of vitamin A acetate for one day. The enhancement lasted for about two weeks, and could be repeated by another stimulation with vitamin A acetate. The enhancing effect of vitamin A acetate was influenced by the cell density. Enhancement was clearly observed when the cell density was under 106 cells/ml. However, when the cell density was over 107 cells/ml, enhancement was observed weakly or not at all. Although the enhancing effect of vitamin A acetate is not unique to AE6 cells, not all human–human hybridoma cell lines show increased productivity upon VA acetate stimulation. This study suggests that the response to vitamin A acetate may be related to the properties of a particular fusion partner which the hybridoma cell inherits. The efficacy of vitamin A acetate for production of human monoclonal antibodies using human–human hybridomas is discussed.
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  • 32
    ISSN: 1573-0778
    Keywords: adaptation ; antibody production rate ; hybridoma ; intracellular amino acids ; osmotic pressure
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    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The time length required for the adaptation of AFP-27 hybridoma cells to high osmotic pressure and the effect of a gradual increase of osmotic pressure on monoclonal antibody production were investigated. When the cells were subjected to an increase of osmotic pressure from 300 mOsmol kg-1 to 366 mOsmol kg- 1, the intracellular content of osmoprotective free amino acids reached a maximum level 6 h after the osmotic pressure was increased to 366 mOsmol kg-1. The same time period of 6 h incubation at 366 mOsmol kg-1 was required to obtain a high growth rate of AFP-27 cells at 440 mOsmol kg-1 when the cells were subjected to a two-step increase of osmotic pressure from 300 mOsmol kg-1 to 366 mOsmol kg-1 and then to 440 mOsmol kg-1. The time length for the physiological adaptation of the cells to 366 mOsmol kg-1 was consequently estimated to be 6 h. Osmotic pressure during batch cultivation was gradually increased from 300 mOsmol kg-1 to 400 mOsmol kg-1 with an adaptation time of at least 6 h. The specific growth rates following a gradual increase of osmotic pressure were higher than those at a constant osmotic pressure of 400 mOsmol kg-1, while the specific monoclonal antibody production rate increased with the increase in the mean osmotic pressure. As a result, the cells grown under a gradual increase of osmotic pressure produced higher amounts of monoclonal antibodies than did those grown under constant osmotic pressure.
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    ISSN: 1573-0972
    Keywords: Beauveria bassiana ; fermentation ; L-glutaminase
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Beauveria sp. BTMF S10 isolated from marine sediment produced extracellular L-glutaminase. Maximal L-glutaminase yield (46.9 U/ml) was obtained in a medium supplemented with 1% (w/v) yeast extract and sorbitol, 9% (w/v) sodium chloride and 0.2% (w/v) methionine, initial pH 9.0 and at 27  °C after 108 h. This enzyme was inducible and growth-associated.
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    World journal of microbiology and biotechnology 15 (1999), S. 231-234 
    ISSN: 1573-0972
    Keywords: starter-cultures ; garri ; cassava ; fermentation ; carriers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Garri is a popular food in Nigeria derived from the fermentation of the mash obtained from the enlarged root of the cassava plant, Manihot esculenta Crantz. As currently produced, the mash used for garri production is spontaneously fermented; on account of this, there is great variability in the organoleptic properties and the quantities of residual cyanide in the garri from Nigeria. The use of starter cultures can help ensure uniformity in these properties if dry carriers can be found on which the fermentative organisms can survive for extended periods so as to facilitate the transportation of their carriers to the many small and scattered garri producers. We therefore studied the survival, singly or mixed, on dry starchy substrates derived from locally available crops, of Lactobacillus coryneformis, Lact. delbruckii, and Saccharomyces sp., which are associated with garri production, as carriers for these organisms. After 16 weeks of storage, between 75% and 85% of the organisms survived on yam, coco-yam, cassava in that order, whereas between 40 and 65% survived on rice and garri. Refrigeration at 4 °C did not improve the survival of the organisms, when compared to room temperature (30 °C) for the organisms stores on yam, coco-yam, and cassava. However where the organisms were stored on rice and garri, refrigeration improved the survivability of the organisms by between 10 and 20%.
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    World journal of microbiology and biotechnology 15 (1999), S. 425-430 
    ISSN: 1573-0972
    Keywords: Activity ; fermentation ; HA-1-92 ; isolation ; oxohexaene ; Streptomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A new antifungal antibiotic, HA-1-92, was isolated from the biomass of Streptomyces CDRIL-312, by extracting in butanol and further purified by silica gel column chromatography followed by preparative TLC. The antibiotic is presumed to be an oxohexaene macrolide and showed promising antifungal activity against yeasts and filamentous fungi including human and plant pathogens. It was found to be less toxic in mice than known oxohexaenes.
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    ISSN: 1573-8221
    Keywords: nitric oxide ; heat-shock proteins ; hypotension ; adaptation
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    Topics: Biology , Medicine
    Notes: Abstract The role of HSP70 and nitric oxide in antihypotensive effects of thermal adaptation was studied. Western blot analysis and electron paramagnetic resonance were used to determine the contents of HSP70 and nitric oxide. Protective effect of adaptation was evaluated by the limitation of blood pressure drop after heat shock. The formation of protective effects, accumulation of HSP70, and development of the ability to decrease nitric oxide overproduction had similar dynamic patterns and appeared at the same period. Quercetin, an inhibitor of HSP70 synthesis, prevented the development of protective effects. The data suggest that HSP70 accumulated during adaptation prevents heat shock-induced hypotension by restricting NO over-production and interfering with its cytotoxic effects.
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    ISSN: 1573-8221
    Keywords: Ca2+ transport ; α-crystallin ; adaptation ; stress
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    Topics: Biology , Medicine
    Notes: Abstract α-Crystallin, an endogenous low-molecular-weight protein with chaperone activity, exerted protective effects on membrane systems of Ca2+ transport into the sarcoplasmic reticulum of skeletal muscles. Protective action of α-crystallin depended on the body state. This effect was not observed in the control and after adaptation to stress, while after stress, especially against the background of adaptation, α-crystallin increased the rate of Ca2+ transport into the sarcoplasmic reticulum and thermal resistance of Ca2+ pump. The mechanisms of α-crystallin activation during stress are discussed.
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    Bulletin of experimental biology and medicine 128 (1999), S. 794-796 
    ISSN: 1573-8221
    Keywords: stress ; adaptation ; behavioral and somatic indices ; rats
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    Topics: Biology , Medicine
    Notes: Abstract We developed a model of stress (free swimming in a cage) which allows to assess the immediate and long-term effects of emotional stress. This stress induced typical changes in the open field test and ulceration of gastric mucosa. Unlike standard immobilization stress the proposed technique excludes a traumatic factor, it is well reproducible and simple.
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    ISSN: 1573-8221
    Keywords: August and Wistar Rats ; stress ; ulcer formation ; adaptation ; behavior
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    Topics: Biology , Medicine
    Notes: Abstract The incidence of gastric ulceration induced by acute emotional stress in Wistar rats is 3 times higher than in August rats, and the mean number of gastric ulcers in Wistar rats 6.3-fold surpassed that in August rats. Wistar rats predisposed to stress-induced ulceration displayed suppressed locomotor and exploratory activities in the open field test, while August rats had more stable behavioral patterns and enhanced exploratory activity after stress. Short-term preadaptation to hypobaric hypoxia for 6 days attenuated stress-induced gastric ulceration, whereas long-term adaptation (40 days) aggravated the severity of gastric ulcers in August and Wistar rats. The interstrain differences in stress-induced ulceration persisted after adaptation. The data suggest that these differences are related to genetically determined peculiarities of production and metabolism of NO and glucocorticoids in August and Wistar rats.
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    Bulletin of experimental biology and medicine 127 (1999), S. 477-479 
    ISSN: 1573-8221
    Keywords: physical exercise ; stress ; kinin system ; elastase ; adaptation
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    Topics: Biology , Medicine
    Notes: Abstract In rats, adaptation to strenuous exercise was accompanied by phasic changes in the activities of the kallikrein-kinin system, elastase-like proteinases, and proteinase inhibitors, and total antioxidant activity in the serum, myocardium, liver, and cerebral cortex. After 30-min physical exercises, activity of the kallikrein-kinin system decreased in the serum and increased in tissue with parallel activation of elastase-like proteinases in the myocardium and cerebral cortex. After 3-h exercises the activity of the kallikrein-kinin system showed some indications of exhaustion, especially in the myocardium and cerebral cortex. Activities of elastase-like proteinases tended to normal due to activation of α1-proteinase inhibitor and normalization of total antioxidant activity.
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    ISSN: 1573-8221
    Keywords: Ca transport ; cytoplasm ; stress ; adaptation
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    Topics: Biology , Medicine
    Notes: Abstract Acute stress reduces and adaptation to stress enhances thermal resistance of Ca2+ pump of the sarcoplasmic reticulum fraction. Soluble cytoplasmic factors increase the rate of Ca2+ transport into myocardial sarcoplasmic reticulum and its thermal resistance in the stressed, stress-adapted, and control rats, the activating effect being most pronounced during acute stress. Structural and functional mechanisms underlying the protective effect of soluble cytoplasmic factors on membrane-bound enzymes are discussed.
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    Bulletin of experimental biology and medicine 127 (1999), S. 151-154 
    ISSN: 1573-8221
    Keywords: adaptation ; opiatergic system ; isoproterenol-induced damage ; myocardium
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    Topics: Biology , Medicine
    Notes: Abstract Adaptation to hypoxia and short-term immobilization stress, as well as preconditioning with Rhodiolae rosea extract produces pronounced antiarrhythmic and cardioprotective effects in the model of adrenergic damage to the heart. Preliminary blockade of opioid receptor significantly decreases the protective effect of adaptation. Using selective opiate receptor antagonists (naltrindole, ICI 174,864, and norbinaltorphimine) we show that the antiarrhythmic effect of adaptation is mediated predominantly via activation ofk-receptors, and to a lesser extent μ-and σ-receptors.
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    Chemie Ingenieur Technik - CIT 71 (1999) 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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    Chemie Ingenieur Technik - CIT 71 (1999), S. 199-206 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Nanofiltration Membranes for Separation Problems in Organic Solutions.Nanofiltration based on rejection and flux features intermediate between those ultrafiltration and reverse osmosis is one of the technically and scientifically interesting membrane processes with a great future. Moreover, most commercial available nanofiltration membranes are only suitable for separation processes in aqueous solutions. A small number of composite membranes with a highly cross-linked selective layer show a technically interesting resistance towards organic solvents, such as ketones, esters, ethers or alcohols. The present article describes such nanofiltration membranes used for separation of low molecular weight chemical compounds from polar or nonpolar solvents, but also for the removal of organic compound from aqueous solutions. Methods of manufacturing and modifying such solvent stable composite membranes are shown, as are the conditioning of membranes and examples of industrial application.
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    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
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    Notes: CAPE in Practice - Opportunities and Limits from the User's Point of View.CAPE tools comprise computer based tools and methods for the design of chemical processes - the use of which is indispensable in modern chemical engineering. This paper discusses the degree to which CAPE is already applied and whether limitations exist. Within BASF, the CAPE framework is provided by the “ProcessNet”, which integrates numerous simulation and design tools. The engineer relies alternatively on inhouse or commercial software. The advantage of CAPE in terms of efficiency depends not only on complex programs, but also to a large extent on the multiple use of intermediate data and program modules. The ProcessNet also features a hierarchical file structure to support the engineer's desktop management. The CAPE environment is being continuously improved. The main constraints on CAPE today are, for example, the missing link between fluid and solid applications, a deficit in data management and the poor state of development of advanced or special programs. The progress of research in this area is followed with great interest.
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    Notes: Die Sitzung des GVC-Fachausschusses „Prozeß- und Anlagentechnik“ fand am 25. und 26. Oktober 1998 in Kleve statt. Gemeinsam mit dem DECHEMA-Ausschuß „Computeranwendungen in der chemischen Industrie“ und dem GVC-Arbeitskreis „Produktionslogistik“ wurden aktuelle Problemstellungen aus der Industrie und neue Problemlösungsansätze aus der Forschung präsentiert und diskutiert.In seiner kurzen Begrüßung erläuterte der Ausschußvorsitzende H. SCHMIDT-TRAUB einerseits die Zielsetzung der Tagung und verdeutlichte andererseits den Teilnehmern die Ursachen und Konsequenzen des drastischen Rückgangs der Anfängerzahlen in den verfahrenstechnischen Studiengängen, was für Gesprächsstoff in den Pausen sorgte. Die anschließende Tagung umfaßte insgesamt 35 Vorträge in zehn Schwerpunktthemen.
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    Notes: Systematic Comparative Cost Analysis of Separation Processes Based on Distillation and Extraction.Rectification is the most commonly used but not always the most economical method of thermal separation. Above all in the case of closely boiling mixtures it becomes very demanding with regard to both equipment and energy. It is often possible to replace rectification by another energetically more favourable separation process such as liquid-liquid distillation, decantation, or absorption. Owing to the resulting wealth of possibilities for the separation of a mixture it is desirable to gain further knowledge about the economics of individual processes and about the selection of suitable entrainers under given conditions.
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    Notes: Possible Approaches to the Prediction of Residence Time Distributions Although the experimental determination of residence time distributions is based on a black-box method, the signal shape also permits certain conclusions to be drawn about the internal conditions. However, if it is to provide a sole basis for elucidating process steps in a plant this method is soon overtaxed. A deeper theoretical penetration of the pertinent transport processes opens up the way ahead. However, since residence time distributions are based on mass-oriented considerations, substantial advances in fluid mechanics, where location-based considerations predominate, have not led to improved predictive capability. Yet the computer power now available offers a variety of ways of calculating residence-time distributions for a known flow profile, thus permitting a step in the direction of better predictability of residence time distributions. This article presents utilisable methods and evaluates them with regard to their demands on time and effort and their predictive power. Problems arising in connection with the models are discussed.
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    Notes: Die diesjährige interne Sitzung des GVC-Fachausschusses „Gasreinigung“ wurde zusammen mit der Arbeitssitzung des Fachausschusses „Energieverfahrenstechnik“ am 19. und 20. April im Tagungszentrum „Festung Marienburg“ in Würzburg abgehalten.
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