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  • Wiley-Blackwell  (19)
  • 1990-1994  (19)
  • 1
    Electronic Resource
    Electronic Resource
    Transmembrane assemblage of the photoreceptor connecting cilium and motile cilium transition zone contain a common immunologic epitope (1990)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 17 (1990), S. 329-344 
    Details
    ISSN: 0886-1544
    Keywords: cytoskeleton ; glycoconjugates ; axoneme ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The photoreceptor connecting cilium bears a unique transmembrane assemblage which stably links cell surface glycoconjugates with the underlying axonemal cytoskeleton. Structural similarities between the photoreceptor connecting cilium and the transition zone of motile cilia suggests that this assemblage may also be present in motile cilia. Using a subcellular fraction enriched in detergent-extracted photoreceptor axonemes, three high molecular mass glycoconjugates (425, 600, and 700 kD) were previously identified as potential components of the assemblage. Through oligosaccharide characterization and binding of a specific monoclonal antibody, we have verified the localization of the 425 kD glycoconjugate to the transmembrane assemblage. Binding of the lectin peanut agglutinin (PNA) to the 425 kD glycoconjugate on nitrocellulose blots, and to isolated detergentextracted axonemes, was assessed following treatment with the enzymes neuraminidase and O-glycanase. Changes in binding to the 425 kD glycoconjugate precisely paralleled changes in binding to intact axonemes, supporting the hypothesis that the 425 kD glycoconjugate is a component of the transmembrane assemblage. Furthermore, the results suggest that the 425 kD glycoconjugate contains sialated galactose-N-acetylgalactosamine oligosaccharides which are Olinked to the protein backbone. To directly assess the distribution of the 425 kD glycoconjugate, we produced a monoclonal antibody directed against this glycoconjugate. The antibody, K26, recognizes only the 425 kD on transblots of the axoneme fraction. K26 immunoreactivity of intact axonemes is identical to that seen by PNA staining. K26 staining of isolated photoreceptors and whole retina is uniquely localized to the region of the connecting cilium. Thus, in the photoreceptor, the 425 kD is not only a component of the transmembrane assemblage but is also completely restricted to the connecting cilium.Based on morphological similarities, the photoreceptor connecting cilium is thought to be homologous to the transition zone of the motile cilium. As such, we have stained oviduct epithelium with the K26 monoclonal antibody. Immunoreactivity is restricted to the region of the transition zone at the base of motile cilia. This demonstrates that the photoreceptor connecting cilium and motile cilium transition zone are immunologically related.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970170408
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  • 2
    Electronic Resource
    Electronic Resource
    Cytoskeletal alterations in cultured cardiomyocytes following exposure to the lipid peroxidation product, 4-hydroxynonenal (1994)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 28 (1994), S. 119-134 
    Details
    ISSN: 0886-1544
    Keywords: microtubules ; vinculin ; desmin ; sarcolemmal damage ; free radicals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Damage to the cardiac myocyte sarcolemma following any of several pathological insults such as ischemia (anoxia) alone or followed by reperfusion (reoxygenation), is most apparent as progressive sarcolemmal blebbing, an event attributed by many investigators to a disruption in the underlying cytoskeletal scaffolding. Scanning electron microscopic observation of tissue cultured rat neonatal cardiomyocytes indicates that exposure of these cells to the toxic aldehyde 4-hydroxynonenal (4-HNE), a free radical--induced, lipid peroxidation product, results in the appearance of sarcolemmal blebs, whose ultimate rupture leads to cell death. Indirect immunofluorescent localization of a number of cytoskeletal components following exposure to 4-HNE reveals damage to several, but not all, key cytoskeletal elements, most notably microtubules, vinculin-containing costameres, and intermediate filaments. The exact mechanism underlying the selective disruption of these proteins cannot be ascertained at this time. Colocalization of actin indicated that whereas elements of the cytoskeleton were disrupted by increasing length of exposure to 4-HNE, neither the striated appearance of the myofibrils nor the lateral register of neighboring myofibrils was altered. Monitoring systolic and diastolic levels of intracellular calcium ([Ca2+]i) indicated that increases in [Ca2+]i occurred after considerable cytoskeletal changes had already taken place, suggesting that damage to the cytoskeleton, at least in early phases of exposure to 4-HNE, does not involve Ca2+ -dependent proteases. However, 4-HNE-induced cytoskeletal alterations coincide with the appearance of, and therefore suggest linkage to, sarcolemmal blebs in cardiac myocytes.Although free radicals produced by reperfusion or reoxygenation of ischemic tissue have been implicated in cellular damage, these studies represent the first evidence linking cardiomyocyte sarcolemmal damage to cytoskeletal disruption produced by a free radical product. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970280204
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  • 3
    Electronic Resource
    Electronic Resource
    Complex intermolecular interactions maintain a stable linkage between the photoreceptor connecting cilium axoneme and plasma membrane (1994)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 28 (1994), S. 213-230 
    Details
    ISSN: 0886-1544
    Keywords: axoneme-plasmalemma cross-linkers ; cytoskeleton-linked glycoconjugates ; cytoskeleton-membrane interactions ; hydrophobic interactions ; lectin cytochemistry ; SDS-resistance ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Microtubule-membrane cross-linkers in motile and nonmotile cilia are supramolecular structures, held together by strong interactions between the constituent molecules. We have characterized these interactions in the photoreceptor connecting cilium, where cross-linkers co-fractionate and maintain their in situ location after Triton X-100 extraction of axonemes. In bovine photoreceptor cells, the transmembrane assemblage that is cross-linked to the connecting cilium axoneme contains three high molecular mass glycoconjugates of 425, 600, and 700 kDa (Horst et al., 1987). The relative amounts of the three glycoconjugates, as judged from band intensity in electrophoretograms, depend strongly on sample treatment prior to electrophoresis. The electrophoretic pattern was reproducible after several weeks of storage of the axoneme fraction in extraction buffer containing 50% sucrose. Removal of sucrose from the buffer by dialysis eliminated the 600 kDa and 700 kDa, and decreased the detected amount of the 425 kDa glycoconjugate. When samples were incubated in Laemmli sample buffer at increasing temperatures (23°, 60°, 95°C), a gradual reduction in the intensity of the three bands was observed. The quantitative reduction of high molecular mass glycoconjugates was accompanied by the appearance of novel protein species of lower molecular mass, as detected by lectin and antibody overlays of axonemal transblots. These results suggest that the previously characterized cross-linker glycoconjugates are complex, SDS-resistant multi-molecular conglomerates. We have further used fluorescent lectins to monitor the presence of glycoconjugates on whole-mounted axonemes, in conditions aimed to selectively solubilize the cross-linkers. The cross-linker complexes could not be dissociated from the axoneme by incubation with buffers containing 1 M of either Na2SO4 or NaI. The results indicate that the connecting cilium-specific cross-linker complexes are bound via high-affinity interactions to both axoneme and overlying plasma membrane. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970280305
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  • 4
    Electronic Resource
    Electronic Resource
    Similarity between flashing and nonflashing two-phase flows (1990)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 36 (1990), S. 797-800 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690360520
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  • 5
    Electronic Resource
    Electronic Resource
    Venting of runaway reactions with gas generation (1992)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 38 (1992), S. 723-732 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pressure relief venting of a runway reaction with gas generation is examined in terms of the overall system response and the associated venting requirement. This article presents an exact formulation as well as an approximate analytical approach. The latter is shown to be particularly useful in vent-sizing applications. Using an aqueous hydrogen peroxide decomposition example, the analytical results are demonstrated to agree well with the exact numerical results over a wide range of overpressure. In addition, the analytical result is shown to reduce to the correct limit for a pure vapor system and offers a useful vent-sizing equation for a pure-gassy system.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690380509
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  • 6
    Electronic Resource
    Electronic Resource
    Removal of VOCs from groundwater using membrane-assisted solvent extraction (1994)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 40 (1994), S. 166-177 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A membrane-assisted solvent extraction (MASX) system coupled to a membrane-assisted distillation stripping (MADS) system for use in decontaminating ground-water is evaluated. Volatile organic compounds (VOCs) in the groundwater are extracted in the MASX unit using a sunflower oil solvent. In the MADS unit, VOCs are stripped from the sunflower oil, which is recycled to the MASX unit. Thermodynamic data for the sunflower oil-water-VOCs system were measured. The results were used along with published membrane mass-transfer data to design MASX and MADS modules.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690400119
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  • 7
    Electronic Resource
    Electronic Resource
    meso-(Tetraphenylporphinato)manganese(III)-tetracyanoethenide, [MnIIITPP]::⊕[TCNE].⊖. A New Structure-Type Linear-Chain Magnet with a Tc of 18KWe gratefully acknowledge support from the U.S. Department of Energy Division of Materials Science Grant No. DE- FG02-86ER45271.A000. We appreciate the synthetic assistance supplied by C. Vazquez, SQUID magnetic susceptibility by D. M. Groski, and R. B. Flippen, insightful discussions with G. T. Yee and P. Zhou, as well as the X-ray diffraction assistance by W. Marshall.Dedicated to Fred Basolo (1992)
    Weinheim : Wiley-Blackwell
    Advanced Materials 4 (1992), S. 498-501 
    Details
    ISSN: 0935-9648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/adma.19920040710
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  • 8
    Electronic Resource
    Electronic Resource
    Cooperative magnetic behavior of α- and β-manganese(III) phthalocyanine tetracyanoethenide (1:1), [MnIIIPc]·⊕[TCNE]·⊖Science and Engineering Laboratories contribution No. 6639. We gratefully acknowledge support from the U. S. Department of Energy Division of Materials Science Grant Nos. DE-FG02-86ER45271. A000 and DE-FG03-93ER45504. We appreciate the SQUID magnetic susceptibility measurements by D. M. Groski and R. B. Flippen, as well as the X-ray diffraction assistance by W. Marshall and N. L. Jones. (1994)
    Weinheim : Wiley-Blackwell
    Advanced Materials 6 (1994), S. 217-221 
    Details
    ISSN: 0935-9648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/adma.19940060306
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  • 9
    Electronic Resource
    Electronic Resource
    ARCO chemical's HAZOP experience (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 12 (1993), S. 83-91 
    Details
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Of all the analytical tools available to the process safety professional, perhaps the most critical are those used to identify and analyze process hazards. Such tools have been collectively referred to as “Hazard Evaluation Procedures” by the Center for Chemical Process Safety (CCPS) and as techniques for “Process Hazard Analysis” (PHA) by the Occupational, Safety and Health Administration (OSHA).One of the best known PHA tools, the Hazard and Operability or HAZOP study, emerged over two decades ago in ICI, U.K., and its use has since spread over six continents. In 1979, ARCO Chemical piloted its first HAZOP and since then, HAZOPs have become the backbone of the company's Process Hazards Review procedure.Repeated use of the HAZOP technique since 1979 has resulted in an affirmation that to be “successful”, much more was needed than simply executing the HAZOP technique. Success is dependent upon the preparation and planning effort that precedes the HAZOP, and the follow-up activity that ensures implementation of study findings. Unlike the study technique, little information was available in 1979 on how to design the framework, or management system, that was needed to support the use of this tool.The purpose of this paper is shift focus from executing the HAZOP technique to that framework, by presenting insights that have accumulated from using the technique in ARCO Chemical, especially during the period 1979 through 1986. These were the formative years, during which the company's advancement on the learning curve was most noticeable. These were the years that convinced the company that successful HAZOP studies do not just happen; success comes from building the right management system. Success must be defined and assured at each step in a Process Hazards Review procedure, of which executing the HAZOP technique is only one.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prs.680120205
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  • 10
    Electronic Resource
    Electronic Resource
    Complexes of tricoordinate hypervalent pnictogens with pentamethylcyclopentadienylrutheniumContribution No. 4422 (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Heteroatom Chemistry 1 (1990), S. 407-417 
    Details
    ISSN: 1042-7163
    Keywords: Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The complexes of a pentamethylcyclopentadienylruthenium moiety with hypervalent tricoordinate pnictogens are reported. A unique mode of complexation is observed for each of the different pnictogens (P, As, Sb). The phosphorus derived complex exhibits an 8-electron tetrahedral bonding environment at phosphorus. The antimony derived complex maintains a 10-electron bonding system at antimony with a pseudo-trigonalbipyramidal geometry at antimony. The arsenic-containing complex is formed with destruction of the original arsenic heterocycle and formation of a trinuclear Ru-Ru-As ring. Remarkably, the formation of the arsenic ruthenium complex can be reversed to reconstruct the original arsenic heterocycle.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hc.520010509
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