ISSN:
1573-5028
Schlagwort(e):
glucuronidase reporter
;
heat shock element
;
heat shock promoter
;
seed desiccation
;
transgenic tobacco
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The soybean Gmhsp17.3-B heat shock promoter is developmentally regulated in transgenic tobacco, as indicated by the constitutive expression of a β-glucuronidase reporter in seeds [16]. In this paper, we show that both the heat shock promoter-driven β-glucuronidase activity and the mRNA of the endogenous Nthsp18P gene accumulate coincident with the onset of seed desiccation. Deletions of the soybean Gmhsp17.3-B promoter, encompassing the heat shock element (HSE)-containing regions, revealed a co-localization of sequences responsible for heat induction and developmental expression. Moreover, synthetic HSEs fused to a TATA box sequence had the potential to stimulate the developmental expression of a GUS reporter gene in seeds of transgenic plants.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00020615
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