ISSN:
1573-5028
Keywords:
development
;
glucuronidase reporter
;
heat shock genes
;
in situ hybridization
;
tissue specificity
;
transgenic plants
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The heat shock (hs) response during plant growth and development was analyzed in tobacco and Arabidopsis using chimaeric β-glucuronidase reporter genes (hs-Gus) driven by a soybean hs promoter. Fluorimetric measurements and histochemical staining revealed high Gus activities in leaves, roots, and flowers exclusively after heat stress. The highest levels of heat-inducible expression were found in the vascular tissues. Without heat stress, a developmental induction of hs-Gus was indicated by the accumulation of high levels of Gus in transgenic tobacco seeds. There was no developmental induction of hs-Gus in Arabidopsis seeds. In situ hybridization to the RNA of the small heat shock protein gene Athsp17.6 in tissue sections revealed an expression in heat-shocked leaves but no expression in control leaves of Arabidopsis. However, a high level of constitutive expression of hs gene was detected in meristematic and provascular tissues of the Arabidopsis embryo. The developmental and tissue-specific regulation of the hs response is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00042039
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