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  • 1
    Electronic Resource
    Electronic Resource
    Large-scale cultivation of Solanum chrysotrichum cells: Production of the antifungal saponin SC-1 in 10-l airlift bioreactors (1997)
    Springer
    Plant cell reports 16 (1997), S. 653-656 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsSolanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of two different cell lines: ccvx (cotyledon derived) and ccvz (hypocotyl derived) of Solanum chrysotrichum were cultivated in 10-l airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l–1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with the ccvx cell line (14.6 g l–1) than with ccvz (7.7 g l–1), resulting in 23 and 12 mg g–1 of SC-1 after 17 days in culture for ccvx and ccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l–1 day–1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l–1 day–1, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite of S. chrysotrichum on a large scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050296
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molybdenum and iron as functional constituents of the enzymes of the nitrate-reducing system of Azotobacter chroococcum (1975)
    Springer
    Archives of microbiology 102 (1975), S. 91-94 
    Details
    ISSN: 1432-072X
    Keywords: Azotobacter ; Iron ; Molybdenum ; Nitrate ; Nitrite ; Tungsten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The roles of molybdenum and iron in the enzymes of the assimilatory nitrate-reducing system from Azotobacter chroococcum have been investigated. 1. By adding 99Mo-molybdate to a cell culture of A. chroococcum with nitrate as the nitrogen source, it has been possible to inccrporate the radioactive metal into a purified preparation of the enzyme nitrare reductase. 2. When 185W-tungstate was supplied to a culture medium lacking added molybdate, a 185W-labelled nitrate reductase preparation with negligible activity could be obtained. This in vivo incorporation of tungsten was competitively hindered by molybdenum. 3. The cellular level of nitrite reductase activity gradually increased in response to the addition of increasing amounts of iron to the culture medium. Under the same conditions, the level of nitrate reductase activity was not affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428351
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  • 3
    Electronic Resource
    Electronic Resource
    Large-scale cultivation ofSolanum chrysotrichum cells: Production of the antifungal saponin SC-1 in 10-I airlift bioreactors (1997)
    Springer
    Plant cell reports 16 (1997), S. 653-656 
    Details
    ISSN: 1432-203X
    Keywords: Solanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of two different cell lines:ccvx (cotyledon derived) andccvz (hypocotyl derived) ofSolanum chrysotrichum were cultivated in 10-1 airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l-1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with theccvx cell line (14.6 g l-1) than withccvz (7.7 g l-1), resulting in 23 and 12 mg g-1 of SC-1 after 17 days in culture forccvx andccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l-1 day-1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l-1 day, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite ofS. chrysotrichum on a large scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275509
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    Paper (German National Licenses)
    Fulltext
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