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Digitale Medien

Expression of the Saccharomyces cerevisiae CYT2 gene, encoding cytochrome c1 heme lyase (1994)

Zollner, Alfred ; Rödel, Gerhard ; Haid, Albert

Springer

Current genetics 25 (1994), S. 291-298

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ISSN: 1432-0983

Schlagwort(e): Cytochrome c 1 ; Cytochrome c 1 heme lyase ; GRF2p ; Glucose repression ; HAPp ; Saccharomyces cerevisiae

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract In this paper we examine the expression of the Saccharomyces cerevisiae CYT2 gene, which encodes cytochrome c 1 heme lyase. This enzyme is required for covalent attachment of heme to apocytochrome c 1, a subunit of the mitochondrial respiratory chain. Transcription of the 1-kb CYT2 mRNA initiates at four prominent sites at a distance of 52–225 bp in front of the AUG start codon. The level of CYT2 mRNA is not influenced by the presence or absence of oxygen or of heme, but it is subject to carbonsource control. The concentration of the CYT2 mRNA is significantly reduced in glucose-grown cells as compared to cells grown under non-repressing conditions. Neither the HAPp activator proteins nor MIG1p, a repressor protein involved in glucose repression, seem to mediate this effect.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00351480

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Digitale Medien

Expression of yeast cytochrome C1 is controlled at the transcriptional level by glucose, oxygen and haem (1992)

Oechsner, Ulrich ; Hermann, Hannes ; Zollner, Alfred ; [weitere]

Springer

Molecular genetics and genomics 232 (1992), S. 447-459

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ISSN: 1617-4623

Schlagwort(e): Saccharomyces cerevisiae ; Cytochrome c 1 ; Promoter dissection ; HAP1, HAP2 transcription factors ; Centromere and promoter-binding factor (CPF1)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary The nuclear gene for cytochrome c 1 in Saccharomyces cerevisiae (CYT1) was localized on chromosome XV. Its upstream region was identified by functional complementation. Fusion to the lacZ reporter gene on a CEN plasmid allowed study of the effect of carbon sources and of specific deletion mutations on expression of the gene in yeast transformants. Detailed promoter analysis combined with expression studies in recipient strains defective in regulatory genes identified cis-acting sites and transcription factors involved in the regulated expression of the cytochrome c 1 gene. These analyses showed that, in the presence of glucose, transcription of CYT1 is positively controlled by oxygen, presumably through the haem signal, and mediated by the HAP1-encoded transactivator. It is additionally regulated by the HAP2/3/4 complex which mediates gene activation mainly under glucose-free conditions. Basal transcription is, in part, effected by CPF1, a centromere and promoter-binding factor.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00266250

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