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Expression of the Saccharomyces cerevisiae CYT2 gene, encoding cytochrome c1 heme lyase

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Abstract

In this paper we examine the expression of the Saccharomyces cerevisiae CYT2 gene, which encodes cytochrome c 1 heme lyase. This enzyme is required for covalent attachment of heme to apocytochrome c 1, a subunit of the mitochondrial respiratory chain. Transcription of the 1-kb CYT2 mRNA initiates at four prominent sites at a distance of 52–225 bp in front of the AUG start codon. The level of CYT2 mRNA is not influenced by the presence or absence of oxygen or of heme, but it is subject to carbonsource control. The concentration of the CYT2 mRNA is significantly reduced in glucose-grown cells as compared to cells grown under non-repressing conditions. Neither the HAPp activator proteins nor MIG1p, a repressor protein involved in glucose repression, seem to mediate this effect.

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Authors and Affiliations

  1. Institut für Genetik und Mikrobiologie, Universität München, Maria-Ward-Strasse 1a, D-80638, München, Germany

    Alfred Zollner & Albert Haid

  2. Labor für Molekulare Biologie und Allgemeine Pathologie, Institut für Pathologie, Universität Ulm, Albert-Einstein-Allee 11, D-89081, Ulm, Germany

    Gerhard Rödel

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  1. Alfred Zollner
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  2. Gerhard Rödel
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  3. Albert Haid
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Communicated by K.Wolf

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Zollner, A., Rödel, G. & Haid, A. Expression of the Saccharomyces cerevisiae CYT2 gene, encoding cytochrome c1 heme lyase. Curr Genet 25, 291–298 (1994). https://doi.org/10.1007/BF00351480

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  • DOI: https://doi.org/10.1007/BF00351480

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