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  • Articles  (58)
  • Springer  (58)
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  • Articles  (58)
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  • 1
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mouse spermatogenic cells are known to express HSP70-2, a member of the HSP70 family of heatshock proteins. The purpose of the present study was to characterize further the expression and localization of HSP70-2 in meiotic cells of mice and hamsters. After separating mouse spermatogenic cells into cytoplasmic and nuclear fractions, proteins were separated by two-dimensional gel electrophoresis and detected with HSP-specific antibodies. Of several HSP70 proteins identified in the cytoplasm, only HSC70 and HSP70-2 were also detected in the nucleus. Immunocytological analyses of spermatocyte prophase cells revealed that HSP70-2 was associated with the synaptonemal complex. Surfacespread synaptonemal complexes at pachytene and diplotene stages labeled distinctly with the antiserum to HSP70-2. Synaptonemal complexes from fetal mouse oocytes failed to show any evidence of HSP70-2. Reverse-transcriptase-polymerase chain reaction (RT-PCR) analyses of gene expression confirmed this sex specificity; Hsp70-2 mRNA was detected in mouse testes, but not ovaries. These findings are suggestive of a previously unsuspected sexual dimorphism in structure and/or function of the synaptonemal complex.
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  • 2
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Mouse spermatogenic cells are known to express HSP70-2, a member of the HSP70 family of heat-shock proteins. The purpose of the present study was to characterize further the expression and localization of HSP70-2 in meiotic cells of mice and hamsters. After separating mouse spermatogenic cells into cytoplasmic and nuclear fractions, proteins were separated by two-dimensional gel electrophoresis and detected with HSP-specific antibodies. Of several HSP70 proteins identified in the cytoplasm, only HSC70 and HSP70-2 were also detected in the nucleus. Immunocytological analyses of spermatocyte prophase cells revealed that HSP70-2 was associated with the synaptonemal complex. Surface-spread synaptonemal complexes at pachytene and diplotene stages labeled distinctly with the antiserum to HSP70-2. Synaptonemal complexes from fetal mouse oocytes failed to show any evidence of HSP70-2. Reverse-transcriptase-polymerase chain reaction (RT-PCR) analyses of gene expression confirmed this sex specificity; Hsp70-2 mRNA was detected in mouse testes, but not ovaries. These findings are suggestive of a previously unsuspected sexual dimorphism in structure and/or function of the synaptonemal complex.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 65 (2000), S. 514-521 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Numerische Mathematik 76 (1997), S. 463-477 
    ISSN: 0945-3245
    Keywords: Mathematics Subject Classification (1991):65F35
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Summary. According to the methodology of [6], many measures of distance arising in problems in numerical linear algebra and control can be bounded by a factor times the reciprocal of an appropriate condition number, where the distance is thought of as the distance between a given problem to the nearest ill-posed problem. In this paper, four major problems in numerical linear algebra and control are further considered: the computation of system Hessenberg form, the solution of the algebraic Riccati equation, the pole assignment problem and the matrix exponential. The distances considered here are the distance to uncontrollability and the distance to instability.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Numerische Mathematik 69 (1994), S. 17-23 
    ISSN: 0945-3245
    Keywords: Mathematics Subject Classification (1991):65F35, 15A12
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Summary. We consider the problem of minimizing the spectral condition number of a positive definite matrix by completion: $$\min\left\{ {\rm cond}\left(\mat{cc} A & B^{\rm H} \\ B & X \rix\right): \mat{cc} A & B^{\rm H} \\ B & X \rix \mbox {\rm positive definite} \right\},$$ \noindent where $A$ is an $n\times n$ Hermitian positive definite matrix, $B$ a $p\times n$ matrix and $X$ is a free $p\times p$ Hermitian matrix. We reduce this problem to an optimization problem for a convex function in one variable. Using the minimal solution of this problem we characterize the complete set of matrices that give the minimum condition number.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 97 (1998), S. 267-274 
    ISSN: 1432-2242
    Keywords: Key words Tiller number ; Developmental behavior ; Quantitative trait loci (QTL) ; Rice (Oryza sativa L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A doubled-haploid rice population of 123 lines from Azucena/IR64 was used for analyzing the developmental behavior of tiller number by conditional and unconditional QTL mapping methods. It was indicated that the number of QTLs significantly affecting tiller number was different at different measuring stages. Many QTLs controlling tiller growth identified at the early stages were undetectable at the final stage. Only one QTL could be detected across the whole growth period. By conditional QTL mapping, more QTLs for tiller number could be detected than that by unconditional mapping. The temporal patterns of gene expression for tiller number could be different at different stages. Even an individual gene or genes at the same genomic region might have opposite genetic effects at various growth stages.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 100 (2000), S. 1295-1303 
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; AFLP markers ; RFLP markers ; Aluminum tolerance ; QTLs ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To investigate the genetic background for aluminum (Al) tolerance in rice, a recombinant inbred (RI) population, derived from a cross between an Al-sensitive lowland indica rice variety IR1552 and an Al-tolerant upland japonica rice variety Azucena, was used in culture solution. A molecular linkage map, together with 104 amplified fragment length polymorphism (AFLP) markers and 103 restriction fragment length polymorphism (RFLP) markers, was constructed to map quantitative trait loci (QTLs) and epistatic loci for Al tolerance based on the segregation for relative root length (RRL) in the population. RRL was measured after stress for 2 and 4 weeks at a concentration of 1mM of Al3+ and a control with a pH 4.0, respectively. Two QTLs were detected at both the 2nd and the 4th weeks on chromosomes 1 and 12 from unconditional mapping, while the QTL on chromosome 1 was only detected at the 2nd stress week from conditional mapping. The effect of the QTL on chromosome 12 was increased with an increase of the stress period from 2 to 4 weeks. The QTL on chromosome 1 was expressed only at the earlier stress, but its contribution to tolerance was prolonged during growth. At least one different QTL was detected at the different stress periods. Mean comparisons between marker genotypic classes indicated that the positive alleles at the QTLs were from the Al-tolerant upland rice Azucena. An important heterozygous non-allelic interaction on Al tolerance was found. The results indicated that tolerance in the younger seedlings was predominantly controlled by an additive effect, while an epistatic effect was more important to the tolerance in older seedlings; additionally the detected QTLs may be multiple allelic loci for Al tolerance and phosphorus-uptake efficiency, or for Al and Fe2+ tolerance.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Rock mechanics and rock engineering 23 (1990), S. 261-273 
    ISSN: 1434-453X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: Summary First, Class II behaviour of rock is discussed with a spring model. The model is characterized by non-uniform failure, which agrees qualitatively with common experimental observation, and shows not only class I but also class II behaviour depending on strength variation of springs. Focusing on the difference between class I and class II behaviour in actual rocks, unloading-reloading tests were conducted in the post-failure region. From the test results, it was found that for both class I and class II rocks, the elastic strain tends to decrease in the post-failure region as the loadbearing capacity deteriorates. The results also show that a remarkable difference between class I and class II categories is the magnitude of non-elastic strain. That is, if non-elastic strain increases faster that elastic strain decreases, then rock shows class I behaviour, and in the opposite case class II behaviour. In general, the non-elastic strain increases with confining pressure and in some cases, rock behaviour changes from class II to class 1 at higher confining pressure.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 205 (1996), S. 121-133 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Systematically complicated technique used for preparing high-intensity (more than 8.0 GBq/cm2)241Am γ-source by a new enamel technique is presented. High intensity241Am γ-sources with activities ranging from 3.7 to 37 GBq have been made by this new technique. The activity and photon output have been measured. The results were compared with the data reported by the Radiochemical Centre Amersham in their specification. The photon output of241Am γ-source produced by us meets the technical specification of241Am γ-sources produced by Amersham. Moreover, the highest intensity can reach 1789 mCi/cm2. The overall utilization ratio of241Am activity (59.5 keV) is 31.2%.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 32 (1997), S. 5661-5667 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The morphology of rubber toughened poly(methyl methacrylate) (PMMA) films was studied during deformation using environmental scanning electron microscopy (ESEM) combined with real time small angle X-ray scattering (SAXS). These two methods provide two different approaches to an in situ study of the morphology of the deforming polymer film. The ESEM study shows that the polymer film exhibits multiple craze-like streaks under tensile strain. Every streak runs through several apparently cavitated rubber particles. Further study by SAXS reveals that these streaks are not crazes. Instead they appear to be lines of cavitated rubber particles which form a particular type of dilatation band known as a “croid”. The ESEM study also shows that with increasing strain the rubber particles gradually increase in size, and that both the number and size of the croids increase. Finally the croids evolve into cracks just before the breakdown of the sample.
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