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  • 1
    Electronic Resource
    Electronic Resource
    Inheritance of two endosperm protein loci in rice (Oryza sativa L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 788-792 
    Details
    ISSN: 1432-2242
    Keywords: Rice (Oryza sativa L.) ; Seed protein loci ; Codominance ; Inheritance ; SDS-PAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Previous studies indicated two types of phenotypic protein markers as two minor bands of SDS-PAGE for rice storage protein. A variant derived from a Pakistani variety, Dular, was found to show a mobility variant with Band 11, a relatively faster-moving band as compared to Band 10, while most of the other cultivated rices exhibited Band 10 at a molecular weight of around 100–110 K. Band 11 was also observed in several wild rice species. How this variant occurred is not known. Another marker is characterized by the presence of either Band 56 (slower-migrating band) or Band 57 (faster-migrating band) in most cultivars at a molecular weight of about 28–27 K. Most indica varieties developed in Taiwan have Band 57 and japonica varieties have Band 56. Genetic analysis of F1, F2 and F3 seeds from interstrain crosses indicated that Band 10 versus Band 11 and Band 56 versus Band 57 are due to codominant alleles at two loci. Tests of independent inheritance between these two loci (Band 10/11 versus Band 56/57) indicated that there is no linkage between them. Both of these two protein loci encode for endosperm proteins and mostly belong to the minor polypeptide subunits of the glutelin fraction of rice seed proteins. Studies on reciprocal crosses indicate dosage effects as exhibited in band patterns. Variations in band intensity were frequently observed when the maternal genotype was different.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266659
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  • 2
    Electronic Resource
    Electronic Resource
    Observed regional distribution of sulfur dioxide in Asia (1995)
    Springer
    Water, air & soil pollution 85 (1995), S. 2289-2294 
    Details
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract SO2 concentrations have been measured for one year at forty-five locations throughout Asia using passive samplers. Duplicate samples were exposed at each site for one month intervals. The sites were selected to provide background information on the distribution of SO2 over wide geographical regions, with emphasis on the regional characteristics around areas estimated to be sensitive to sulfur deposition. The annual mean values ranged from less than 0.3 μg/m3 at Tana Rata, located at 1545 m on the Malaysia Peninsula, Lawa Mandau, (Borneo) Malaysia, and Dhankuta, Nepal, to values greater than 20 μg/m3 at Luchongguan (Guiyang) China, Babar Mahal, Nepal, and Hanoi, Vietnam. In general high concentrations were measured throughout China, with the highest concentrations in the heavy industrial areas in Guiyang. The concentrations in east Asia around the Korea peninsula were ∼5 μg/m3. The concentrations in the southeast Asia tropics were low, with no station in Malaysia and Indonesia having average concentrations exceeding 1.7 μg/m3. The observed SO2 concentrations were found to display a distinct seasonal cycle which is strongly influenced by the seasonality of winds and precipitation patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01186175
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  • 3
    Electronic Resource
    Electronic Resource
    Mössbauer study of melt-spun Sm20Fe70Ti10 alloy (1992)
    Springer
    Hyperfine interactions 69 (1992), S. 585-588 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The rapidly queoching samples of Sm20Fe70Ti10 were prepared by melt spinning. The coercivities of crystallized ribbons were found to vary with the spinning velocity. The value of coercivity obtained was 3.2kOe, which is higher than that reached in Sm−Fe−Ti alloys with the ThMn17 structure. The results of Mössbauer effect and X ray diffraction analyses indicate that the alloy consists of SmFe2, SmFe3, an undetermined paramagnetic phase and the minor phase of Sm2Fe17.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02401895
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  • 4
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    Observed regional distribution of sulfur dioxide in Asia (1995)
    Springer
    Details
    Publication Date: 1995-12-01
    Print ISSN: 0049-6979
    Electronic ISSN: 1573-2932
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Springer
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    PAPER CURRENT
  • 5
    Electronic Resource
    Electronic Resource
    Compound Poisson approximation for unbounded functions on a group, with application to large deviations (1995)
    Springer
    Probability theory and related fields 103 (1995), S. 515-528 
    Details
    ISSN: 1432-2064
    Keywords: 60B10 ; 60F05 ; 60F10 ; 60B15
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Summary A second order error bound is obtained for approximating ∫h d $$\tilde Q$$ by ∫h d $$\tilde Q$$ , where $$\tilde Q$$ is a convolution of measures andQ a compound Poisson measure on a measurable abelian group, and the functionh is not necessarily bounded. This error bound is more refined than the usual total variation bound in the sense that it contains the functionh. The method used is inspired by Stein's method and hinges on bounding Radon-Nikodym derivatives related to $${{d\tilde Q} \mathord{\left/ {\vphantom {{d\tilde Q} {dQ}}} \right. \kern-\nulldelimiterspace} {dQ}}$$ . The approximation theorem is then applied to obtain a large deviation result on groups, which in turn is applied to multivariate Poisson approximation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01246337
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  • 6
    Electronic Resource
    Electronic Resource
    Electrophysiological Study of Chimeric Sodium Channels from Heart and Skeletal Muscle (1998)
    Springer
    The journal of membrane biology 164 (1998), S. 25-34 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Rat skeletal muscle — Human heart — Chimeric channel — Expression — Cell line — Patch-clamp — Sodium channels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. The α-subunit cDNAs encoding voltage-sensitive sodium channels of human heart (hH1) and rat skeletal muscle (rSkM1) have been expressed in the tsA201 mammalian cell line, in which inactivation properties appear to be normal in contrast to Xenopus oocytes. A series of rSkM1/hH1 chimeric sodium channels has been evaluated to identify the domains of the α-subunits that are responsible for a set of electrophysiological differences between hH1 and rSkM1, namely, midpoints and slope factors of steady-state activation and inactivation, inactivation kinetics and recovery from inactivation kinetics and their voltage-dependence. The phenotype of chimeric channels in which each hH1 domain was successively introduced into a rSkM1 α-subunit framework confirmed the following conclusions. (i) The D4 and or/C-ter. are responsible for the slow inactivation of hH1 sodium channels. (ii) Concerning the other differences between rSkM1 and hH1: steady-state activation and inactivation, kinetics of recovery from inactivation, the phenotypes are determined probably by more than one domain of the α-subunit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002329900390
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  • 7
    Electronic Resource
    Electronic Resource
    A protocol for consistent, large-scale production of fertile transgenic rice plants (1998)
    Springer
    Plant cell reports 18 (1998), S. 25-31 
    Details
    ISSN: 1432-203X
    Keywords: Key words Rice ; Microparticle bombardment ; Genetic transformation ; Embryogenic tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol for consistent production of fertile transgenic rice plants was established utilizing microparticle bombardment of embryogenic tissues (Oryza sativa L. japonica cv. Taipei 309). This system has been employed to produce several thousand independently transformed plant lines carrying the hygromycin phosphotransferase (hph) gene and various genes of interest. The most efficient target tissue was highly embryogenic callus or suspension cell aggregates, when they were given an osmotic pre- and post-transformation treatment of 0.6 m carbohydrate. By optimizing the age of the tissue at the time of gene transfer and applying an improved selection procedure, transgenic plants were recovered in 8 weeks from the time of gene transfer, at an average of 22.3±9.7 per 100 calli and 22.4±8.0 plant lines per dish of suspension cell aggregates. This system has facilitated a number of studies using rice as a model for genetic transformation and will enable the large-scale production of transgenic rice plants for genomic studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050526
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  • 8
    Electronic Resource
    Electronic Resource
    Protoplast fusion between Lycopersicon esculentum and L. peruvianum-complex: somatic embryogenesis, plant regeneration and morphology (1998)
    Springer
    Plant cell reports 17 (1998), S. 508-514 
    Details
    ISSN: 1432-203X
    Keywords: Key words Protoplast fusion ; Iodoacetamide ; Somatic embryogenesis ; Morphology ; Chromosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic hybrids were obtained by polyethylene glycol fusion of cotyledon protoplasts of Lycopersicon esculentum Mill. cv. Kyoryokutoko treated with iodoacetamide (IOA) and suspension-culture-derived protoplasts of L. peruvianum (PI270435) or L. chilense (PI128652). The hybrids were selected by a multiple-step selection procedure relying on the different colors of the fusion partners, IOA treatment of cotyledon protoplasts, and the use of a culture medium which only allowed cotyledon protoplasts to regenerate. The somatic embryos were derived from greenish calli that formed from the fusion mixtures, developed progressively through the globular, heart, and torpedo stages, and finally formed complete plantlets. The excised torpedo-stage embryos could be propagated on a modified medium. The morphology of the somatic hybrids were intermediate to their donor partners, and chromosome observations indicated that the hybrids were tetraploid, hexaploid, and aneuploid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050433
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  • 9
    Electronic Resource
    Electronic Resource
    Identification and purification of a spinach chloroplast DNA-binding protein that interacts specifically with the plastid psaA-psaB-rps14 promoter region (1997)
    Springer
    Planta 203 (1997), S. 373-380 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Chloroplast ; DNA-binding protein (puri fication) ; Operon ( psaA) ; Spinacia (chloroplast) ; Trans-acting factor ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. We have previously shown the presence in chloroplasts of sequence-specific DNA-binding proteins that interact specifically with two regions located downstream and upstream from the 5′-transcription start site of the plastid psaA-psaB-rps14 operon. As part of an effort to elucidate the regulatory mechanism of plastid transcription during plant development, we report here the purification and characterization of the chloroplast DNA-binding protein from spinach (Spinacia oleracea L. var. spinosa Ashers et Graeden) leaves that specifically recognizes sequences between positions +64 to +83 relative to the transcription start site. This DNA-binding protein has been highly purified from chloroplasts by using a combination of high-salt extraction, ammonium sulfate precipitation, heparin-agarose chromatography, and sequence-specific DNA-affinity chromatography. The protein exhibited an apparent molecular weight of 59–60 kDa on the basis of gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Southwestern blot analysis further indicated that this DNA-binding protein is dimeric and composed of two ≈31-kDa subunits. We discuss the properties of this protein in relation to the known chloroplast DNA-binding factors for plastid gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050203
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  • 10
    Electronic Resource
    Electronic Resource
    Discharge patterns of chicken cochlear ganglion neurons following kanamycin-induced hair cell loss and regeneration (1994)
    Springer
    Journal of comparative physiology 174 (1994), S. 351-369 
    Details
    ISSN: 1432-1351
    Keywords: Cochlear ganglion neurons ; Hair cell regeneration ; Tuning curves ; Spontaneous activity ; Threshold
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Hair cells in the basal, high frequency region (〉1100 Hz) of the chicken cochlea were destroyed with kanamycin (400 mg/kg/d × 10 d) and allowed to regenerate. Afterwards, single unit recordings were made from cochlear ganglion neurons at various times post-treatment. During the first few weeks post-treatment, only neurons with low characteristic frequencies (〈1100 Hz) responded to sound. Despite the fact that the low frequency region of the cochlea was not destroyed, neurons with low characteristic frequencies had elevated thresholds, abnormally broad U-shaped or W-shaped tuning curves and low spontaneous discharge rates. At 2 days post-treatment, the spontaneous discharge rates of some acoustically unresponsive units fluctuated in a rhythmical manner. As recovery time increased, thresholds decreased, tuning curves narrowed and developed a symmetrical V-shape, spontaneous rate increased and neurons with higher characteristic frequencies began to respond to sound. In addition, the proportion of interspike interval histograms with regularly spaced peaks increased. These improvements progressed along a low-to-high characteristic frequency gradient. By 10–20 weeks post-treatment, the thresholds and tuning curves of neurons with characteristic frequencies below 2000 Hz were within normal limits; however, the spontaneous discharge rates of the neurons were still significantly lower than those from normal animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240217
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