ISSN:
1432-2048
Keywords:
Petrocoptis
;
Petroglaucin
;
Petrograndin
;
Ribosome-inactivating protein
;
rRNA N-glycosidase
;
Translation (inhibition)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two new N-glycosidase type-1 ribosome-inactivating proteins (RIPs), denoted petroglaucin 1 and petrograndin, respectively, were isolated from the plantsPetrocoptis glaucifolia (Lag.) Boiss sp.viscosa (Rothm.) Laínz andPetrocoptis grandiflora Rothm. These new RIPs do not share H2N-terminal amino-acid sequence homology with petroglaucin (now denoted as petroglaucin 2), the only other type-1 RIP to be isolated fromP. glaucifolia (Arias et al. (1992) Planta186, 532–540). Petroglaucin 1 shares amino-acid sequence homology with RIPs from Cucurbitaceae while petroglaucin 2 and petrograndin do so with saporins and dianthin 30 (Caryophyllaceae). The new RIPs strongly inhibited protein synthesis at subnanomolar concentrations in rabbit reticulocyte lysates and other eukaryotic cell-free systems, but they were inactive on bacterial ribosomes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00714460
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