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Isolation and characterization of two new N-glycosidase type-1 ribosome-inactivating proteins, unrelated in amino-acid sequence, from Petrocoptis species

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Abstract

Two new N-glycosidase type-1 ribosome-inactivating proteins (RIPs), denoted petroglaucin 1 and petrograndin, respectively, were isolated from the plantsPetrocoptis glaucifolia (Lag.) Boiss sp.viscosa (Rothm.) Laínz andPetrocoptis grandiflora Rothm. These new RIPs do not share H2N-terminal amino-acid sequence homology with petroglaucin (now denoted as petroglaucin 2), the only other type-1 RIP to be isolated fromP. glaucifolia (Arias et al. (1992) Planta186, 532–540). Petroglaucin 1 shares amino-acid sequence homology with RIPs from Cucurbitaceae while petroglaucin 2 and petrograndin do so with saporins and dianthin 30 (Caryophyllaceae). The new RIPs strongly inhibited protein synthesis at subnanomolar concentrations in rabbit reticulocyte lysates and other eukaryotic cell-free systems, but they were inactive on bacterial ribosomes.

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Abbreviations

RIP:

ribosome-inactivating protein

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Authors and Affiliations

  1. Departamento de Bioquímica, Biología Molecular y Fisiología, Facultad de Ciencias, Universidad de Valladolid, E-47005, Valladolid, Spain

    F. Javier Arias, M. Angeles Rojo, J. Miguel Ferreras, Rosario Iglesias, Raquel Muñoz, Fernando Soriano & Tomás Girbés

  2. Servicio de Endocrinología, Centro Ramón y.Cajal, Carretera de Colmenar Viejo, E-28034, Madrid, Spain

    Enrique Méndez

  3. Dipartimento di Patologia Sperimentale, Università di Bologna, I-40126, Bologna, Italy

    Luigi Barbieri

Authors
  1. F. Javier Arias
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  2. M. Angeles Rojo
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  3. J. Miguel Ferreras
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  4. Rosario Iglesias
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  5. Raquel Muñoz
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  6. Fernando Soriano
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  7. Enrique Méndez
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  8. Luigi Barbieri
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  9. Tomás Girbés
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Additional information

This work was supported by grants from CICYT (BIO89-0705 and BIO92-0231) and the Junta de Castilla y León (Spain) to T. Girbés and FIS (92-0858) to E. Méndez. We wish to thank N. Skinner (University of Salamanca, Salamanca, Spain) for correcting the English version of the manuscript. The technical assistance of J. E. Basterrechea is also greatly appreciated.

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Arias, F.J., Rojo, M.A., Ferreras, J.M. et al. Isolation and characterization of two new N-glycosidase type-1 ribosome-inactivating proteins, unrelated in amino-acid sequence, from Petrocoptis species. Planta 194, 487–491 (1994). https://doi.org/10.1007/BF00714460

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