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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Risk analysis 8 (1988), S. 0 
    ISSN: 1539-6924
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Previous applications of carcinogenic risk assessment using mathematical models of carcinogenesis have focused largely on the case where the level of exposure remains constant over time. In many situations, however, the dose of the carcinogen varies with time. In this paper, we discuss both the classical Armitage–Doll multistage model and the Moolgavkar–Venzon–Knudson two–stage birth–death–mutation model with time-dependent dosing regimens. Bounds on the degree of underestimation of risk that can occur through the use of a simple time-weighted average dose are derived by means of comparison with an equivalent constant dose corresponding to the actual risk under the time-dependent dosing regimen.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1439-0329
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions.
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  • 3
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 730 (1994), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 653 (1992), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 38 (1989), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Red raspberry plants were subjected to waterlogging after inoculation with different species of Phytophthora isolated from field outbreaks of root rot. The commonest species, a form of P. megasperma, was highly pathogenic to raspberry whether the plants had been waterlogged or not, but waterlogging increased the severity of disease caused by other species. P. cambivora and P. drechsleri were moderately pathogenic and non-pathogenic respectively in non-waterlogged conditions but killed plants that had been waterlogged for 4 days. P. megasperma var. megasperma required longer periods of waterlogging to cause severe symptoms and did not kill plants. Root rotting of waterlogged and non-waterlogged plants was controlled with a metalaxyl and copper mixture. The results indicated that several species of Phytophthora may contribute to poor growth and death of raspberries grown in poorly drained sites.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 40 (1991), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Zoospores and agar discs of the fungus causing raspberry root rot (Phytophthora fragariae) were used as inoculum to assess the resistance of raspberry genotypes in pot experiments. High zoospore concentrations resulted in more severe symptoms than low concentrations. There was a strong correlation between the results obtained with zoospores and with colonized agar discs as inocula. although disease was generally more severe with the former. The severity of root rot was correlated with a number of other symptoms, in particular growth reductions, stem lesions and leaf wilting, and some of these symptoms could possibly be used as measures of resistance. There was no evidence of physiological specialization with the range of isolates and raspberry genotypes used. Pot tests in general gave results which matched reported resistances in the field, and could be used to assess the resistance of breeding lines and selections.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 40 (1991), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Hypersensitivity and susceptibility of potato leaflets of different genotypes to Phytophthora infestans were distinguished using ELISA. Estimates of pathogen growth indicated that an intermediate reaction sometimes occurred. Visual symptoms of disease were not always closely related to the pathogen content (mycelium, μg fresh weight) of leaflets estimated by ELISA. Leaflets inoculated with 1000 zoospores in a droplet had to be incubated for at least 6-8 days at 15 C before reliable estimates of the reaction by ELISA could be determined. Increasing the inoculum load improved the differentiation between hypersensitivity and susceptibility.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 37 (1988), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The virulences of 102 single-zoospore cultures of Phytophthora fragariae from one field site were determined on a range of strawberry differentials, and used to assign the cultures to four clusters (I, II A, IIB, IIC) using cluster analysis. On cv. Favourite, which is susceptible to all known races of the pathogen, isolates in cluster I were recovered most frequently and had the narrowest spectrum of virulence. On cv. Saladin isolates in cluster IIB were more common and were pathogenic to this cultivar. However, c. 30% of the single zoospores from field isolates from Saladin were avirulent on this cultivar and belonged to cluster I. Hyphal-tip and single-zoospore cultures from selected field isolates in cluster IIB did not always have the same virulence phenotype as the parent isolates. One hyphal-tip culture from a field isolate in cluster IIB had a virulence phenotype (IID) which had not been recorded before in Europe, and it attacked some cultivars not previously affected by red core. When cultivars such as Saladin were inoculated with mixtures of zoospores from two isolates from different clusters, with and without the corresponding virulence factors, the isolate with the corresponding virulence factor was selected. However, on the universally susceptible cv. Favourite the results depended on the relative competitiveness of the isolates and not on virulence factors.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 36 (1987), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Phytophthora syringae, P. drechsleri, P. cactarutn, P. cambiuora and P. megasperma were isolated from the roots of red raspberry plants affected by severe root and crown rot with associated cane death. Phytophthora megasperma occurred most frequently and consisted of two types of isolates which differed in colony morphology, growth rates, and oogonial, oospore, sporangial and zoospore size, and pathogenicity to a range of plants. One type with large oospores was typical of P. megasperma var. megasperma, and was non-pathogenic to red raspberries, while the other with smaller oospores and which grew more slowly in culture than the first, was highly pathogenic, producing symptoms similar to those observed in the field. Highly pathogenic isolates from Germany and the USA were of this type.All red and black raspberry cultivars tested were susceptible to the pathogenic type, although North American cultivars were generally less affected than British ones. Inoculated plants had reduced shoot and root weights, stem lesions and wilted and yellowed leaves. The blackberry × raspberry hybrid Tayberry and its blackberry parent were immune.Phytophthora drechsleri, P. cactorum and P. cambivora produced small to moderate amounts of root rot on red raspberry, and P. cambivora also caused slight symptoms on shoots.
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