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  • 1
    Electronic Resource
    Electronic Resource
    Comparative transcription analysis of higher plant plastome mutants (1988)
    Springer
    Current genetics 14 (1988), S. 171-176 
    Details
    ISSN: 1432-0983
    Keywords: Pelargonium ; Plastome mutants ; Plastid RNA ; Northern analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Several plastome mutants ofPelargonium zonale hort. are characterized by either plastid ribosome deficiencies of specific defects in their photosystems. Southern analyses of plastid DNA fragments containing both the rDNA region and therbcL gene revealed no differences between wild-type and mutant leaves. Northern analyses of mutants with plastid ribosome deficiencies indicate lower levels of plastid ribosomal RNA and mRNA coding for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in mutant leaves relative to green leaves. The data presented in this paper indicate a defect at the transcriptional level in the plastid ribosome deficient plants. We suggest that a RNA polymerase defect affects transcriptional efficiency in these mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569341
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  • 2
    Electronic Resource
    Electronic Resource
    Hybrid variegation in the genus Pelargonium (1982)
    Springer
    Current genetics 5 (1982), S. 245-249 
    Details
    ISSN: 1432-0983
    Keywords: Hybrid variegation ; Pelargonium ; Extranuclear inheritance ; Plastid DNA ; Restriction patterns
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary. 1) The genetical and cytological analysis of crosses between the wild-type form ‘Roseum’ of Pelargonium zonale (L.) L 'Herit. ex Ait. and the hybrid species Pelargonium zonale hort. ‘Stadt Bern’' revealed the occurrence of hybrid variegation in the genus Pelargonium 2) The plastids in the green-white variegated plants were sorted out in the quick apical way characteristic for Pelargonium chimera. Six types of periclinal chimeras could be observed. 3) The DNAs of the two different plastomes can be distinguished by restriction endonuclease analysis with EcoRl and BamHI. 4) The differences in the cleavage patterns allowed a detailed description of the process of sorting out of the two parental plastids types within the variegated hybrids on the level of their plastid DNA. In all cases observed the cleavage pattern of ‘Roseum’ plastid DNA was found in the green tissue and the pattern of ‘Stadt Bern’ plastid DNA in the white tissue. 5) From these results we draw the conclusion that there is a disharmony between the F1-hybrid nucleus and the plastome of Stadt Bern', which blocks the normal differentiation of ‘Stadt Bern’ plastids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391813
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  • 3
    Electronic Resource
    Electronic Resource
    Variations of chloroplast DNAs in the genus Pelargonium and their biparental inheritance (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 37-41 
    Details
    ISSN: 1432-2242
    Keywords: Pelargonium ; Inter- and intra-specific chloroplast DNA variation ; Restriction patterns ; Biparental plastid inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The comparison of EcoRI patterns of chloroplast DNAs (ctDNAs) from five species of the genus Pelargonium and from 16 cultivars and varieties of Pelargonium zonale hort. demonstrates a remarkable inter- and intraspecific ctDNA (plastome) variation. The plastome of the P. zonale varieties could be differentiated into groups I, II and III. Reasons for this variation seem to be: occurrence of numerous spontaneous plastome mutations, intense hybridisation by gardeners and breeders, and biparental plastid inheritance. Crosses of P. zonale varieties with different ctDNA types lead to the direct evidence on the molecular level of biparental plastid inheritance and plastid sorting-out in F1-hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275175
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  • 4
    Electronic Resource
    Electronic Resource
    Sequence analysis and Escherichia coli minicell transcription test of Pelargonium plastid 5S rDNA (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 514-516 
    Details
    ISSN: 1617-4623
    Keywords: Pelargonium ; Plastid 5S rDNA ; Sequence ; Minicells ; Not transcribed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sequence of the plastid 5S rDNA of Pelargonium zonale hort. “Roseum” is presented. In the 5′ region of this gene there is almost no homology to the Escherichia coli promoter consensus sequences. In an E. coli minicell system we have demonstrated that the plastid promoter of the 16S rDNA is active in E. coli but no separate transcript of the cloned 5S rDNA can be detected. These results provide new evidence that the plastid 5S rDNA of P. zonale and probably also of other higher plants is not transcribed as a separate unit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331624
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